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Hybrid culture method for two-color lily magnolia

A culture method and double-color technology, applied in the field of plant tissue culture, can solve the problems of difficult survival in breeding, easy browning of explants, and low survival rate, and achieve the effects of improving the survival rate of culture, high rooting rate, and high survival rate

Inactive Publication Date: 2015-11-25
罗光珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These cultivation methods take a long time, and the breeding is not easy to survive
Some of the existing two-color Vermilion flower culture methods are carried out under closed aseptic conditions, and the required cost is relatively large, and there is also the problem that the explants are easy to brown in tissue culture and the survival rate is not high, so they cannot be used for the cultivation of Vermilion flower. Multiply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Use bacteriostatic agents to sterilize and bacteriostabilize the site where the two-color Zhu Jinhua is cultivated for one or more of bactericidal fruit, mancozeb and hypochlorous acid;

[0017] The selection of explants: choose the robust growth with axillary buds with a diameter of 1.5cm, and the branches of Zhu Jinhua without disease and insect poisoning are explants, cut off the leaves of the branches, and cut them into 1.5cm stems with axillary buds part;

[0018] Disinfection and sterilization of explants: first rinse the explants with tap water for 6 minutes, then immerse the explants in a 0.1% mercury solution, add 2ml Tween solution, soak the explants in the mixed solution for 7 minutes, and finally rinse the explants 4 times with sterile water;

[0019] Initial induction culture: Soak the sterilized explants in 2% PVP for 2 minutes, then insert the stem section upwards into the WPM basic medium, and carry out initial culture at 22°C and light intensity 2000Lx...

Embodiment 2

[0024] Use bacteriostatic agents to sterilize and bacteriostabilize the site where the two-color Zhu Jinhua is cultivated for one or more of bactericidal fruit, mancozeb and hypochlorous acid;

[0025] The selection of explants: choose the robust growth with axillary buds with a diameter of 1.5cm, and the branches of Zhu Jinhua without disease and insect poisoning are explants, cut off the leaves of the branches, and cut them into 1.5cm stems with axillary buds part;

[0026] Disinfection and sterilization of explants: first rinse the explants with tap water for 8 minutes, then immerse the explants in a mercuric chloride solution with a concentration of 0.1%, add 2.5ml Tween solution, and soak the explants in the in the mixed solution for 10 minutes, and finally rinse the explants 5 times with sterile water;

[0027] Initial induction culture: Soak the sterilized explants in 2% PVP for 4 minutes, then put the stem segment upwards in WPM basic medium, and carry out initial cul...

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PUM

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Abstract

The invention provides a hybrid culture method for two-color lily magnolia. The culture method for the two-color lily magnolia is performed in an open condition. The method comprises the steps of sterilization and bacterial inhibition treatment of a two-color lily magnolia culture place, explant selection, explant sterilization and disinfection, explant primary culture, multiplication culture, rooting culture and the like. As the culture method for the two-color lily magnolia can be performed in the open condition, the cost is reduced; and for sterilization of the lily magnolia, no bacterial pollution of explants is ensured and the survival rate of culture is increased.

Description

technical field [0001] The invention relates to the field of plant tissue culture, and mainly relates to a tissue culture method of Zhu Jinhua. Background technique [0002] Zhujinhua, also known as magnolia and magnolia, is a unique plant in China. It is distributed in Yunnan, Fujian, Hubei, Sichuan and other places in China. It grows in areas with an altitude of 300 to 1600 meters, and generally grows on the edge of hillsides. The flowers of Zhu Jinhua are gorgeous and pleasant, with elegant fragrance, and they are beautiful alone or in clusters. They are excellent garden and street greening plants, and they are also traditional Chinese flowers and traditional Chinese medicines. However, Zhu Jinhua is not easy to transplant and maintain. It has been included in the "World Nature Conservation" Union" plant red list, is a very precious flowers and trees. [0003] Now with the development of technology, many areas are carrying out the method of cultivating Zhu Jinhua, and wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 罗光珍
Owner 罗光珍
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