Magnolia liliiflora tissue culture method

A tissue culture and purple magnolia technology, applied in the field of plant tissue culture, can solve the problems of easy browning of explants, difficulty in breeding, low survival rate, etc., and achieve the effect of improving the survival rate of culture, short time and high survival rate

Active Publication Date: 2014-04-16
哈威光电科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These cultivation methods take a long time, and the breeding is not easy to survive
Some existing purple magnolia tissue culture methods are carried out under closed aseptic conditions, the required cost is relatively large, and there is also the problem that the explants are easy to brown in tissue culture and the survival rate is not high, so they cannot be used for purple magnolia. Mass reproduction of magnolia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Use bacteriostatic agents to sterilize and bacteriostatically treat the site where the magnolia tissue culture is carried out for one or more of the sterilizing fruit, mancozeb and hypochlorous acid;

[0017] The selection of explant: choose the vigorous growth that has the axillary bud of 1.5cm in diameter, the Purple Magnolia branch without disease and insect poisoning is explant, cut off the blade of branch, and it is cut into the stem section of the band axillary bud of 1.5cm ;

[0018] Disinfection and sterilization of explants: first rinse the explants with tap water for 6 minutes, then immerse the explants in a 0.1% mercury solution, add 2ml Tween solution, soak the explants in the mixed solution for 7 minutes, and finally rinse the explants 4 times with sterile water;

[0019] Initial induction culture: Soak the sterilized explants in 2% PVP for 2 minutes, then insert the stem section upwards into the WPM basic medium, and carry out initial culture at 22°C and ...

Embodiment 2

[0024] Use bacteriostatic agents to sterilize and bacteriostatically treat the site where the magnolia tissue culture is carried out for one or more of the sterilizing fruit, mancozeb and hypochlorous acid;

[0025] The selection of explant: choose the vigorous growth that has the axillary bud of 1.5cm in diameter, the Purple Magnolia branch without disease and insect poisoning is explant, cut off the blade of branch, and it is cut into the stem section of the band axillary bud of 1.5cm ;

[0026] Disinfection and sterilization of explants: first rinse the explants with tap water for 8 minutes, then immerse the explants in a mercuric chloride solution with a concentration of 0.1%, add 2.5ml Tween solution, and soak the explants in the in the mixed solution for 10 minutes, and finally rinse the explants 5 times with sterile water;

[0027] Initial induction culture: Soak the sterilized explants in 2% PVP for 4 minutes, then put the stem segment upwards in WPM basic medium, and...

Embodiment 3

[0032] Use bacteriostatic agents to sterilize and bacteriostatically treat the site where the magnolia tissue culture is carried out for one or more of the sterilizing fruit, mancozeb and hypochlorous acid;

[0033] The selection of explant: choose the vigorous growth that has the axillary bud of 1.5cm in diameter, the Purple Magnolia branch without disease and insect poisoning is explant, cut off the blade of branch, and it is cut into the stem section of the band axillary bud of 1.5cm ;

[0034] Disinfection and sterilization of explants: first rinse the explants with tap water for 10 minutes, then immerse the explants in a 0.1% mercury solution, add 3ml Tween solution, soak the explants in the mixed solution for 13 minutes, and finally rinse the explants 6 times with sterile water;

[0035] Initial induction culture: Soak the sterilized explants in 2% PVP for 5 minutes, then insert the stem segment upwards into the WPM basic medium, and carry out initial culture at 26°C an...

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PUM

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Abstract

The invention provides a magnolia liliiflora tissue culture method. The magnolia liliiflora tissue culture method is performed under open conditions, and the method comprises the following steps: performing sterilization and bacteriostasis on a magnolia liliiflora tissue culture field, selecting an explant, sterilizing and disinfecting the explant, performing primary culture on the explant, performing multiplication culture and performing rooting culture. The magnolia liliiflora tissue culture method is performed under open conditions and can be performed under the open conditions, and the cost is saved; according to sterilization of the magnolia liliiflora, the explant is not contaminated by bacteria, and the culture survival rate is improved; the explant is soaked by 2 percent of PVP, and the 2 percent of PVP is added into a culture medium, so that browning of the explant is lightened, and the survival rate is improved. The magnolia liliiflora tissue culture method is simple in operation, short in time, low in cost, high in survival rate and high in rooting rate, bottle transplanting in a short time can be realized, desirable traits of the original variety can be kept, and a foundation is laid for seed stock breeding and industrialized production.

Description

technical field [0001] The invention relates to the field of plant tissue culture, and mainly relates to a method for tissue culture of Magnolia magnolia. Background technique [0002] Purple magnolia, also known as magnolia and magnolia, is a unique plant in China. It is distributed in Yunnan, Fujian, Hubei, Sichuan and other places in China. It grows in areas with an altitude of 300 to 1600 meters, and generally grows on the edge of hillsides. Purple magnolia flowers are gorgeous and pleasant, fragrant and elegant, and they are beautiful alone or in clusters. They are excellent gardens and street greening plants, and they are also traditional Chinese flowers and traditional Chinese medicines. However, purple magnolias are not easy to transplant and maintain, and have been included in the "World It is a very precious flower and tree in the Red List of Plants of the IUCN. [0003] Now with the development of technology, many areas are carrying out the method of cultivating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈凤花
Owner 哈威光电科技(苏州)有限公司
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