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218results about How to "Easy to freeze" patented technology

Semiconductor structure

A desired property for a metal gate electrode layer is that it can cover a three-dimensional semiconductor structure having a microstructure with high step coverage. Another desired property for the metal gate electrode layer is that the surface of a deposited electrode layer is flat on a nanometer scale, enables a dielectric layer for electrical insulation to be coated without performing special planization after deposition of the electrode layer. Furthermore, another desired property for the metal gate electrode layer is that it has the similar etching workability to materials used in an ordinary semiconductor manufacturing process. Furthermore, another desired property for the metal gate electrode layer is that it has a structure in which diffusion of impurity is suppressed due to homogeneity thereof and the absence of grain boundaries. It was found that an amorphous metal electrode is most suitable for realizing the metal gate electrode layer satisfying the above-mentioned properties and thereby the present invention was achieved.
Owner:NAT INST OF ADVANCED IND SCI & TECH

Systems and Methods for Cryopreservation of Cells

An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. In another embodiment, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening.
Owner:SEXTON BIOTECHNOLOGIES INC

Semiconductor structure

A desired property for a metal gate electrode layer is that it can cover a three-dimensional semiconductor structure having a microstructure with high step coverage. Another desired property for the metal gate electrode layer is that the surface of a deposited electrode layer is flat on a nanometer scale, enables a dielectric layer for electrical insulation to be coated without performing special planization after deposition of the electrode layer. Furthermore, another desired property for the metal gate electrode layer is that it has the similar etching workability to materials used in an ordinary semiconductor manufacturing process. Furthermore, another desired property for the metal gate electrode layer is that it has a structure in which diffusion of impurity is suppressed due to homogeneity thereof and the absence of grain boundaries. It was found that an amorphous metal electrode is most suitable for realizing the metal gate electrode layer satisfying the above-mentioned properties and thereby the present invention was achieved.
Owner:NAT INST OF ADVANCED IND SCI & TECH

Refrigeration method for preserving embryo and oocyte by vitrification of glasscapillary

This invention discloses a method for preserving embryo and oocyte through vitrification cryopreservation by utilizing capillary glass tube. The method utilizes high borosilicate capillary glass tube, and comprises: (1) preparing freezing liquid; (2) freezing; (3) preparing thawing liquid; (4) thawing. The method utilizes high borosilicate capillary glass tube to preserve embryo and oocyte, and has such advantages as no need for expensive devices, low cost, easy operation, good vitrification cryopreservation effect, and high survival rate after thawing.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI

Medical Systems, Methods, and Devices for Hypopigmentation Cooling Treatments

Embodiments of the present invention generally relate to methods, devices, and systems for reducing a pigmentation of a skin of a patient. In some embodiments, freezing of the skin may be desirable to effect the hypopigmentation of the skin of the patient. Generally, embodiments may limit supercooling (or promote freezing) of the skin of the patient during a cooling treatment. In some embodiments, coupling fluids are provided to reduce a thermal contact resistance between a cooling treatment probe and the skin of the patient to improve cooling treatment. Optionally, a fluid carrier may be provided to help retain the coupling fluid at the treatment site. In some embodiments, the coupling fluid may include ice nucleating agents to promote ice crystal formation in the coupling fluid during cooling treatment. The ice crystal formation in the coupling fluid may progress into the skin to limit supercooling of the skin during treatment.
Owner:R2 TECHNOLOGY INC

Micro-electro-mechanical system (MEMS) strain type icing sensor and detection method

The invention discloses a micro-electro-mechanical system (MEMS) strain type icing sensor and a detection method, and relates to icing thickness detection technologies. The MEMS strain type icing sensor comprises a dual-rectangle-shaped frame, a peripherally clamped square flat film, detection resistors, reference resistors, an ice melting resistor and electrode leads. A detection system is formed by the MEMS strain type icing sensor and an interface circuit. When an icing phenomenon is detected, a Wheatstone bridge is formed by the detection resistors and the reference resistors, and the detection resistors are used to sense stress changes of the surface of the flat film, which are caused by the icing, thereby achieving the aim of icing detection. On the one hand, the interface circuit is used for providing constant current drive for the Wheatstone bridge; and on the other hand, the interface circuit is used for amplifying voltage signals output by the bridge and conducting temperature compensation on the voltage signals.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI

Solidification method for preparing para-aramid by adopting dry jet-wet spinning

The invention provides a solidification method for preparing para-aramid by adopting dry jet-wet spinning. The method is characterized by comprising the following specific steps: extruding a PPTA-H2SO4 spinning solution from a spinneret plate; enabling liquid flow extruded from the spinneret plate to vertically pass through an air layer and feeding to a solidification bath; leading out through a godet wheel, so as to obtain a nascent fiber; and washing, winding and drying, so as to obtain a para-aramid fiber precursor, wherein the solidification bath is sulfuric acid solution and is formed by sulfuric acid and water; the mass fraction of the solidification bath is 2.0%-15.0%; and the temperature of the solidification bath is 1-12 DEG C. The fineness of the fiber can be significantly reduced under the condition of the solidification bath; the strength of the para-aramid fiber is improved; the apparent defects of the fiber can be reduced; and the fiber quality is improved, thus the stability of a spinning process and the quality of a fiber product can be effectively improved.
Owner:DONGHUA UNIV

Copious cooling pulverizer and working method thereof

InactiveCN106111289AGuarantee that the mesh meets the requirementsEasy to collectGrain treatmentsLiquid nitrogen containerNitrogen gas
The invention relates to a cryogenic pulverizer and its working method, comprising a motor, a hopper, a material pre-cooling room, a material freezing room, a low-temperature mill, an induced draft fan, a cyclone separator, a bag filter, a liquid nitrogen tank and a compressor. Machine, the cryogenic pulverizer of the present invention and its working method, use liquid nitrogen to cool and freeze the material to be pulverized so as to achieve the brittleness required for the pulverization of the material, rich in resources and easy to separate, colorless, odorless and non-toxic gas, to avoid In addition, nitrogen is also a good anti-oxidant, which can prevent the finished powder material from being oxidized and deteriorated, and realize the recycling of nitrogen. In addition, the cooling capacity of the finished powder material is fully utilized to reduce energy consumption. save resources.
Owner:菏泽市花王高压容器有限公司

Freezing apparatus

The present invention relates to a freezer unit. It includes a heat-insulating box body, feeding mechanism and discharge mechanism which are respectively placed in material inlet and material outlet of said heat-insulating box body, at least two circulation conveying mesh-belts mounted in the heat-insulating box body interior, evaporator positioned in heat-insulating box body interior and cold-air machine formed from fan group. The cold-air outlet of cold-air machine is communicated with air-collecting cavity positioned under the circulation conveying mesh-belt, the liquor supply tube of the evaporator and return tube are respectively connected with refrigerating machine group, first circulation conveying mesh-belt is driven by mechanical combined reciprocating vibration feeding device, and other circulation conveying mesh-belt is driven by electric speed-reducing machine. Said invention is high in freezing efficiency.
Owner:SIFANG TECH GRP CO LTD

Ovarian large cortex piece vitrified cryopreservation protection liquid and cryopreservation method thereof

InactiveCN102771471APromote perfusion timeImprove biological activityDead animal preservationOvarian transplantationIn vivo
The invention relates to an ovarian large cortex piece vitrified cryopreservation protection liquid and a cryopreservation method thereof. Although the vitrified cryopreservation method is widely applied to experimental animals, a universal cryopreservation method and a standard cryopreservation liquid preparation scheme are absent at present, so that the sized transplantation subjected to vitrified cryopreservation is at a small-size stage, an ovarian small cortex piece has limited follicles, and the service life of the transplanted follicles is influenced. The invention provides vitrified cryopreservation protection liquid suitable for an ovarian large cortex piece, and gonadotropin is added on the basis of the common vitrified liquid. According to the vitrified cryopreservation protection liquid suitable for the ovarian large cortex piece, especially the ovaries of experimental animals, improved variety livestock and wild endangered species in sudden death are conveniently cryopreserved at the most proper osmotic equilibrium time of performing vitrified cryopreservation on the ovarian large cortex pieces of different sizes; and moreover, according to the method, the survival rate of the follicles can be improved, the blood reperfusion time of the transplant is shortened, and the survival rate of the cryopreserved ovarian transplantation in vivo is improved.
Owner:NINGXIA MEDICAL UNIV

Water-saving tailing discharge method

ActiveCN105350498AEmission buildup is easy to controlImprove stabilityVortex flow apparatusSoil drainageTerrainWater saving
The invention belongs to the field of metallurgical mines, and particularly relates to a water-saving tailing discharge method. A place is selected according to the tailing pond address selection principle. A tailing pond, a primary dam, a floodwater drainage system, a seepage drainage system, a tailing conveying system, a tailing classification and condensation system, a water return system and other matched facilities are reasonably arranged according to terrain and geological conditions. The tailing conveying system comprises a section from a dressing plant to a hydrocyclone, a section from the hydrocyclone to a thickener, and a section form the underflow of the thickener to the tailing pond. Pumping or self-flowing conveying can be adopted for the conveying process of each section. The tailing classification and concentration involves the steps that ore pulp coming from the dressing plant is classified through the hydrocyclone; and overflow tailing ore pulp of the hydrocyclone is concentrated through the thickener. The water return system comprises a thickener overflow water return system and a tailing pond water return system. Return water can be conveyed to the mineral processing process to be recycled in a pumping or self-flowing conveying manner. The tailing pond formed through the method is good in anti-seismic property, high in return water utilization rate, low in dam body seepage line, large in anti-slide buckling safety factor, wide in application range and low in operation and management expense.
Owner:金建工程设计有限公司

Android process freezing and unfreezing device and method

The invention discloses an Android process freezing and unfreezing device and method, and relates to the technical field of mobile terminals. The device comprises a freezing request module, a freezing processing module and an unfreezing module, wherein the freezing request module is used for sending a freezing request to an application process by a mobile terminal through communication; the application process comprises a work thread and a communication thread; the freezing processing module is used for judging whether the connection between the application process and another application process is completely processed or not through the communication thread, and freezing the work thread and keeping the activity state of the communication thread if the judging result is positive; and the unfreezing module is used for unfreezing the work thread when the communication thread receives a connection request from another application process, and carrying out freezing and unfreezing on the process through the communication thread. During the freezing, only the work thread is frozen and the communication thread is not frozen, so that the communication thread can be timely unfrozen when required, thereby improving the process freezing and unfreezing efficiencies, and greatly reducing the system risks caused by the freezing and unfreezing behaviors.
Owner:NUBIA TECHNOLOGY CO LTD

Ultra-low temperature preserving method for nutrient breeding flower and short-tube lycoris

InactiveCN1934941AThe cryopreservation method is simple and easyStable and reliable cryopreservation methodDead plant preservationVitrificationShoot
The present invention relates to an ultra-low temperature storage method of nutrient propagative flower plant lycoris radiate. Said method includes the following steps: bulb induction, pre-culture of shoot tip with root rudiment and bud rudiment, placing the above-mentioned pre-cultured shoot tip in pretreatment solution to make pretreatment at room temperature for 10-30 min, then placing it into vitrified protective agent to make ice bath treatment for 70-90 min, then transplanting said shoot tip into a freeze tube in which the precooled fresh vitrified protective agent is held and placing said freeze tube into liquid nitrogen so as to make storage.
Owner:SHANGHAI AGROBIOLOGICAL GENE CENT

Anti-freezing device for air cooling island in power station

The invention relates to exhaust-steam cooling equipment for a steam turbine in the power industry, in particular to an anti-freezing device for an air cooling island in a direct air cooling unit of a power station. The device includes an air cooling island condensation water header (1), a steam calandria radiator (2) and a steam distributing pipe device (3). The device is characterized in that fixed wind shields (5) and (6) are mounted on the periphery at an air outlet side of the air cooling island steam calandria radiator, and a louver-type shield device (4) is mounted above the steam calandria radiator. The device is characterized in that the structure is simple, the adjustment is flexible and convenient, and the response speed is high, solves the defects that the present air cooling island is incapable of adjusting heat preservation manually and the switch response speed of an electric rolling door is lower, further can prevent the problem that the windward side of the air cooling island is frozen easily in freeze-up, and completely solves the difficulties that the air cooling island is frozen to damage and the heat impact causes equipment damage when the standby equipment in freeze-up is put into operation.
Owner:万逵芳

Pavement ground tile

The invention belongs to the field of road construction, and particularly relates to a pavement ground tile. The pavement ground tile comprises a drainage groove, a treading mechanism and buffering mechanisms. The drainage groove is formed in the upper end face of the pavement ground tile, and a drainage pipe which communicates with the exterior is fixedly connected with the bottom of the drainage groove. The treading mechanism is arranged in the drainage groove and comprises a treading plate, an annular limiting groove, protrusions and sliding rods. A magnetic material is adopted by the treading plate, and the treading plate is arranged in a groove opening of the drainage groove. The annular limiting groove is formed in the side wall, close to the groove opening, of the drainage groove, and the edge of the treading plate extends to the annular limiting groove. The protrusions are fixedly connected to the inner top surface of the annular limiting groove, and the lower ends of the protrusions make contact with the edge of the upper end of the treading plate in an abutting mode. The sliding rods are fixedly connected to the interior of the annular limiting groove and arranged on the treading plate in a penetrating and sliding mode. The buffering mechanisms are arranged in the space between the treading plate and the drainage groove. The pavement ground tile is good in buffering effect, high in drainage capacity and outstanding in antiskid effect.
Owner:阳泉市鑫盛瓷业有限公司

Method for preparing instant dried corn steep liquor powder by using corn steep liquor

The invention discloses a method for preparing instant dried corn steep liquor powder by using corn steep liquor. The method comprises the following steps of: separating the corn steep liquor into a permeate and a trapped fluid by an ultrafiltration membrane separation technology, treating a separation liquid with lower molecular weight by a lime milk neutralization method to prepare inositol and produce a supernatant, mixing the supernatant and a separation liquid with high molecular weight, concentrating the mixed liquor by using a 'four-effect evaporation' system, adding a certain amount of maltodextrin into the concentrated mixed liquor, and preparing microencapsulated instant dried corn steep liquor powder through spray drying. By the method, the retention rate of protein is high in the ultrafiltration membrane separation process; during concentration by using the 'four-effect evaporation' system, steam is saved, and energy consumption is reduced; and the corn steep liquor is wrapped by a microcapsulation technology, so that the corn steep liquor is easy to dry, preserve and transport.
Owner:HENAN XINYUAN BIOTECH CO LTD

Gene knockout targeting vector and application thereof

The invention relates to a gene knockout targeting vector, which comprises a positive selective gene. Two ends of the positive selective gene are provided with a recombinant enzyme recognition sequence; the other end of the recombinant enzyme recognition sequence is respectively connected with a 5' homologous arm and a 3' homologous arm of a knockout gene; promoters for promoting the expression of the positive selective gene are not arranged in the two homologous arms; and the outer sides of the homologous arms are provided with negative selective gene. The targeting vector transfects cells, positive clones are screened through a culture medium containing a medicament corresponding to positive selective gene, and the obtained positive clones have high enrichment efficiency; and the cell screening method is simple without massive manpower and material resources, the subsequent cell freezing and identification are greatly facilitated, and the cost of the gene targeting is greatly reduced.
Owner:BEIJING GEFUCURE BIOTECHNOLOGY LIMITED COMPANY

New method for cryopreserving hematopoietic stem cells

The invention discloses a new method for cryopreserving hematopoietic stem cells. The new method comprises the following steps: step 1, taking a hematopoietic stem cell protection agent, a hematopoietic stem cell suspension solution or bone marrow fluid at the volume ratio of (1-2) to 6; step 2, uniformly mixing the hematopoietic stem cell suspension solution or the bone marrow fluid with the hematopoietic stem cell protection agent; emptying air and sealing, and storing the mixed solution in a refrigerator in a sealed manner at -80 DEG C for 12 hours; and step 3, taking out the mixed solution of the hematopoietic stem cell suspension solution or the bone marrow fluid and the hematopoietic stem cell protection agent; and then adding the hematopoietic stem cell protection agent, wherein the volume ratio of the hematopoietic stem cell protection agent to the mixed solution is 1 to 3; and emptying air and sealing again, and storing the mixed solution in the refrigerator in a sealed manner at -80 DEG C. The cryopreserving method provided by the invention is simple and convenient, is easy to operate and has a relatively good cryopreserving effect.
Owner:北京军科联合干细胞生物科技有限公司

Systems and methods for cryopreservation of cells

An auto-nucleating device includes a hollow tube containing a crystalline cholesterol matrix therein. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device is disposed within the vessel and provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within. The opposite end of the vial is initially open to receive the liquid. The end is then sealed to form a closed system for cryopreservation. Another cryopreservation vessel includes an adaptor mounted to a port of a liquid container. The adaptor includes one tubular branch that is closed by a needle septum while another tubular branch includes a barbed fitting for engaging a flexible tube. The flexible term terminates in a needle septum. The container is initially at below-atmospheric pressure. In use, the liquid sample is injected by needle through the septum in the second branch. Once the container is filled with the sample, the flexible tube is heat sealed and severed just above the barbed fitting. The sealed container may then be subject to a cryopreservation protocol. After thawing, the sample liquid may be withdrawn by a needle puncturing the septum in the first branch.
Owner:SEXTON BIOTECHNOLOGIES INC

Complete device used for frozen-breaking and metal sorting of waste lithium batteries and method

The invention discloses a complete device used for frozen-breaking and metal sorting of waste lithium batteries and a method. The complete device used for frozen-breaking and metal sorting of the waste lithium batteries comprises a first automatic feeding device, a liquid nitrogen quick freezing device, a first breaking device, a second automatic feeding device, a second breaking device, a cycloneseparator and a screening device which are arranged in sequence. By means of the complete device and method provided by the invention, frozen-breaking and metal sorting recovery of the waste lithiumbatteries can be achieved, pollution of the waste lithium batteries to the environment is avoided, meanwhile, resource recycling prevents resource wasting, in addition, the hidden trouble that a largeamount of heat is released after short circuit of the lithium batteries and causes combustion is avoided when the waste lithium batteries are broken, and meanwhile, it can be guaranteed that breakingand metal sorting treatment can be efficiently performed on the waste lithium batteries in bulk.
Owner:绵阳鼎天机电科技有限公司

Puffed potato whole meal and preparation method of puffed potato whole meal

The invention discloses puffed potato whole meal and preparation method of the puffed potato whole meal. The puffed potato whole meal is prepared by the following steps: cleaning potatoes; stewing; feeding the stewed potatoes at a cold outdoor air temperature, and freezing; transferring the thoroughly frozen potatoes inside, unfreezing, peeling and dewatering; and airing the dewatered potatoes on a heated brick bed, and preparing powder. The process disclosed by the invention is simplified; the production cost is low; the method is easy to popularize; the prepared puffed potato whole meal product is soft in texture, easy to dry, easy to crush, free of caking when being kneaded, and easy to knead, not easy to go bad and long in retention period and has the relatively white color.
Owner:全洪范

Cell vitrification freezing liquid and freezing method

The invention belongs to the field of freezing and provides a cell vitrification freezing liquid and a freezing method. The freezing liquid comprises a washing liquid, an equilibrium liquid and a vitrifying liquid. With the freezing fluid, the action time in the equilibrium liquid and the vitrifying liquid is shortened, and chemical toxicity of an osmosis protective agent is reduced. In addition,the operation is simple, and the cell survival rate is high.
Owner:陈子江 +1

Device and method for ultrasonic strengthening frozen dough

InactiveCN101455217AShorten the total freezing timeImprove freezing efficiencyDough treatmentCooling cycleEngineering
The invention provides an ultrasonic reinforced freezing dough preparing apparatus and a method thereof.The apparatus of the invention comprises an ultrasonic wave device, a device for detecting temp. and a cooling cycle device; the method of the invention employs ethylene glycol solution as the freezing medium and the ultrasonic propagation medium, and in the process of immersing and freezing of dough, ultrasonic wave is applied.The apparatus has a simple structure and a simple and convenient operation and control. By adopting the apparatus and method of the invention, heat transferring in the freezing course and ice crystal core formation are both reinforced, comparing with the common freezing course, the freezing time of dough is reduced, ice crystals of big amount and small coarseness are formed in the frozen dough; furthermore, springiness of the frozen dough remains excellent and the quality of dough is improved.
Owner:SOUTH CHINA UNIV OF TECH

High-temperature-resistant paint for hydraulic cylinder and preparation method thereof

The invention discloses a high-temperature-resistant paint for a hydraulic cylinder and a preparation method thereof. The high-temperature-resistant paint for the hydraulic cylinder is prepared from the following constituents in parts by weight: 58-60 parts of modified epoxy organic silicon resin, 22-24 parts of hydrogenated acrylonitrile butadiene rubber, 27-29 parts of bisphenol A epoxy resin, 12-14 parts of mica powder, 10-13 parts of butyl rubber, 5-7 parts of polydimethylsiloxane, 12-14 parts of phenylmethylsilane resin, 3-5 parts of dioctyl phthalate, 2-4 parts of copper powder, 2-4 parts of rutile type titanium dioxide, 1-3 parts of ceramic powder, 20-25 parts of deionized water, 2-4 parts of triethylene tetramine, 5-7 parts of triphenyl phosphite, and 1-3 parts of an auxiliary.
Owner:SUZHOU TONGMING MACHINERY

Preparation method of meal replacement powder

The invention discloses a preparation method of meal replacement powder. The preparation method comprises the steps of S1, cereal crushing; S2, cereal sterilization; S3, dry-wet separation; S4, water milling and screening; S5, product collection; S6, quick freezing of the product. The cereals are beaten and crushed, bran remaining on the surfaces of the cereals is removed, and large cereals are rapidly crushed, so that the surfaces of the cereals are clean. Meanwhile, the situation that a discharging port is blocked due to accumulation of large particles is avoided, the bacteria-free condition of subsequent production of the product is guaranteed through continuous sterilization, dry-wet separation is conducted on the sterilized product, a large amount of water is prevented from rapidly entering the grinding position, and therefore the concentration of a powder solution is guaranteed, and the situation that the drying efficiency is affected due to too low concentration is avoided. Dust raising and wet powder accumulation caused by too dry powder are avoided through water milling, normal production of the product is guaranteed, and in addition the production quality of the product is guaranteed.
Owner:东莞市霖鑫牛食品科技有限公司

Air bathing type gasifier easy to deice

The invention discloses an air bathing type gasifier easy to deice. The inlet ends of a plurality of heat exchange pipes are connected with a liquid inlet pipe after being connected in series, the outlet ends are connected with an air discharge pipe after being connected in series, a cross-shaped insert piece is arranged in each heat exchange pipe, fins are vertically and uniformly connected with the outer walls of the heat exchange pipes, wherein the heat exchange pipes are snake-shaped, the longitudinal gaps between the parallel sections shrink gradually along the liquid medium flow direction, and the fin number is increased gradually section by section; each cross-shaped insert piece is spiral so as to enable the liquid low-temperature medium to generate an obvious dispersion flow effect in the heat exchange pipe. According to the air bathing type gasifier easy to deice, the environment temperature at four sides of the air bathing type gasifier easy to deice descends slowly through expanding the longitudinal gaps between parallel sections of the heat exchange pipes near the inlet end and reducing the fin number at the inlet end, so that the air bathing type gasifier is not easy to deice; the air bathing type gasifier easy to deice has beneficial effects of simple structure, convenience in use, high heat exchange efficiency, low cost and the like, and the air bathing type gasifier easy to deice can be broadly used for different large, medium and small enterprises and public institutions.
Owner:WUXI IMPRINT NANO TECH

Multi-enzyme system for separating different tissues-derived primary culture cells of normal human and mammal, application and related kit thereof

The invention relates to a multi-enzyme system for separating different tissues-derived primary culture cells of a normal human and a mammal. The system comprises a plurality of 0.0125 to 0.125 weight percent of trypsin, 0.02 to 0.2 weight percent of collagenase I-IV, 0.0015 to 0.015 weight percent of hyaluronidase, 0.02 to 0.2 weight percent of neutral protease, 0.0015 to 0.015 weight percent of deoxyribonuclease I, 1 to 10 U / ml papain, 0.015 to 0.15 weight percent of pronase, 5 to 50 U / ml elastase and 0.01 to 0.1 weight percent of separase. The invention also relates to the application of the multi-enzyme system and a kit formed by the multi-enzyme system. The multi-enzyme system of the invention has the characteristics of reasonable design and capacity of separating the different tissues-derived primary culture cells of the normal human and the mammal highly specifically and highly sensitively; and the separated primary culture cells have the characteristics of high survival rate, high purity, easy cryopreservation and recovery, can be used for medicament experimental evaluation, medicament toxicity test and medicament therapeutic judgment, and are suitable for large-scale popularization and application.
Owner:刘东旭

Vitrification freezing and storing method of a little somatic cells for cloning domestic animal

The invention discloses a vitrification freezing preservation method of a small aqueous cell used for cloning domestic animals and belongs to the field of vitrification freezing preservation. The operation steps of the method are as follows: (I) liquid preparation; (II) vitrification freezing; (III) thawing. Compared with the prior art, the vitrification freezing preservation method of a small aqueous cell used for cloning domestic animals of the invention has the characteristics of low cost expense, simple method, needing no expensive instruments, good vitrification freezing preservation effect and high survival rate after thawing.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI

Quick freezer

ActiveCN107014139AImprove quick freezing effectNot a single structureLighting and heating apparatusStationary refrigeration devicesCold airEngineering
The invention discloses a quick freezer. The quick freezer comprises a quick freezer shell, an evaporator is installed at the top inside the quick freezer shell, a circulating draught fan is installed on the lower side of the evaporator, a discharging conveyer belt and a feeding conveyer belt are installed inside the quick freezer shell, the discharging conveyer belt is installed at the bottom inside the quick freezer shell and penetrates through a discharging outlet in the left side wall of the quick freezer shell, the feeding conveyer belt is installed below the circulating draught fan and penetrates through a feeding inlet in the right side wall of the quick freezer shell, and a first central conveyer belt, a second central conveyer belt and a third central conveyer belt are sequentially installed between the discharging conveyer belt and the feeding conveyer belt from bottom to top. A condenser installed externally and independently is adopted for heat exchange, the situation that integrated forming affects the refrigerating environment is avoided, a product can be sufficiently refrigerated by installing pressurized cold air delivery pipes and matched air jet heads between the conveyer belts, every two pressurized cold air delivery pipes are located on the two sides of a fixed plate, the quick freezing effect is further enhanced, and two refrigerating modes of the top evaporator and the pressurized cold air delivery pipes are adopted.
Owner:江苏神工智能科技有限公司
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