40 results about "Bone marrow fluid" patented technology

An automatic method for analyzing nucleated bone marrow cells comprising partitioning one sample of bone marrow fluid with two samples, one sample being treated with a first lysing agent and a first staining solution and the other sample being treated with a second lysing agent and a second staining solution; and measuring each samples in a flow cytometer using a scattered light and fluoresence which enables to classify and count leukocytes, erythroid cells and lipid particles, as well as mature myeloid cells, lymphoid cells and immature myeloid cells, and to calculate the number of myeloid cells as well as the ratio of myeloid cells and erythroid cells.

The invention discloses a marrow fluid cell partition method based on depth study, and relates to the field of biological medical image treatment and computer application. Compared with the prior art, the marrow fluid cell partition method provided by the embodiment is realized by a clustering algorithm and a depth study method; the method is direct and easy to practice; through an automatic evaluation system of the partitioning quality, the partitioning result is more exact; the component characteristic of the HSV image is fully used, and the unique regional growth method is designed, and the calculation process is greatly simplified; the efficiency is improved.

The bone marrow harvesting drill of the invention includes an inner needle having a cutting edge at the tip thereof; a tubular mantle that receives the inner needle thereinto so that the inner needle and the tubular mantle are detachably attached; and a lock mechanism that prevents the axial rotation of the tubular mantle and the inner needle relative to each other; the inner needle having a groove formed at the tip thereof projecting from the tubular mantle for discharging bone scraps produced by the cutting edge at the tip of the inner needle; and the tubular mantle having a cutting edge formed at the tip edge thereof and a helical groove extending from the tip edge to at least part of the peripheral surface of the tubular mantle so as to be flush with the groove of the inner needle.

The invention discloses an injectable articular cartilage tissue repair material and is characterized in that starting materials of the composite material contain the following components of: by weight, 100 parts of I-type collagen and 10-50 parts of hyaluronic acid, wherein oxidisability of hyaluronic acid is 20-60%. A monocyte layer in bone marrow fluid in vivo is embedded in the repair material. The thickness of the composite material is 1-5mm. Hyaluronic acid is oxidized into oxidized hyaluronic acid by the use of sodium periodate. The collagen solution, 5*PBS and a buffer are mixed at the volume ratio of 7:2:1 and the pH is adjusted to neutral. After dissolving oxidized hyaluronic acid by the use of ultrapure water, the dissolved oxidized hyaluronic acid is added into the neutral collagen solution and the mixed solution is uniformly stirred and stands in a refrigerator of 4 DEG C. The separated and extracted monocyte layer in the bone marrow fluid by a density gradient centrifugation method is uniformly mixed with the above collagen-oxidized hyaluronic acid solution. The mixed liquor is putted into a constant temperature incubator of 37 DEG C for standing or injection to the organism articular cartilage defects so as to form the composite material.

The invention discloses a new method for cryopreserving hematopoietic stem cells. The new method comprises the following steps: step 1, taking a hematopoietic stem cell protection agent, a hematopoietic stem cell suspension solution or bone marrow fluid at the volume ratio of (1-2) to 6; step 2, uniformly mixing the hematopoietic stem cell suspension solution or the bone marrow fluid with the hematopoietic stem cell protection agent; emptying air and sealing, and storing the mixed solution in a refrigerator in a sealed manner at -80 DEG C for 12 hours; and step 3, taking out the mixed solution of the hematopoietic stem cell suspension solution or the bone marrow fluid and the hematopoietic stem cell protection agent; and then adding the hematopoietic stem cell protection agent, wherein the volume ratio of the hematopoietic stem cell protection agent to the mixed solution is 1 to 3; and emptying air and sealing again, and storing the mixed solution in the refrigerator in a sealed manner at -80 DEG C. The cryopreserving method provided by the invention is simple and convenient, is easy to operate and has a relatively good cryopreserving effect.

The invention relates to a bone marrow aspiration extraction device with a positioning frame. The device comprises a bone marrow aspiration extraction needle and an aspiration positioning frame for auxiliary positioning in an aspiration process, A bone marrow aspiration needle includes a cylindrical housing, a aspiration needle, needle core assembly, Anesthesia needle and pulp withdrawal needle, alancet core assembly include a lancet core body and a lancet control handle, an anesthetic needle comprise an anesthetic syringe, a piston and a push-pull rod arranged in the anesthetic syringe. Thepulp extracting needle comprises a pulp extracting needle barrel, a piston arranged in the pulp extracting needle barrel and a push-pull rod; the anesthetic needle barrel and the pulp extracting needle barrel are respectively passed through the other two channels of the rotary cover; and the aspiration positioning frame comprises a rectangular frame, a positioning block, an aspiration block, a longitudinal positioning strip, a transverse positioning strip and four legs. The bone marrow aspiration needle not only can solve the problem of reducing the aspiration times, but also is provided witha positioning frame, which is convenient for medical personnel to carry out aspiration and bone marrow fluid extraction operation.

The invention relates to a bone marrow aspiration device for the hematology department. The device comprises a support mechanism detachably arranged on an examination bed and a puncture mechanism arranged on the support mechanism, the puncture mechanism comprises a needle body which comprises a movably arranged outer catheter, the outer catheter is communicated with a bone marrow output tube, an inner catheter is movably arranged in the outer catheter, the outer diameter of the inner catheter is smaller than the inner diameter of the outer catheter, one end of the inner catheter is communicated with an anesthetic input tube, the other end of the inner catheter stretches out of the outer catheter and then is fixedly connected with a conical needle tip, the diameter of the large end of the needle tip is equal to the outer diameter of the outer catheter, the side wall of the needle tip is provided with an outlet communicated with the inner catheter, a soft blocking piece is arranged on the outer side of the outlet, and the lower portion of the soft blocking piece is fixedly connected with the needle tip. According to the bone marrow aspiration device for the hematology department, thepositioning accuracy is higher, and the phenomenon that anesthetic contaminates a bone marrow fluid cannot occur.

The invention provides a dried bone floss comprising the constituents (by weight proportion) of the following raw materials, 40-60 portions of fresh bones and boiled bone marrow, 40-60 portions of meat and conventional auxiliary materials. The invention also provides a process for preparing the dried bone floss which comprises, (1) cleaning the fresh bones, boiling, separating, (2) grinding, filtering, drying, (3) heating and mixing into fibrous mud form, (4) drying, baking, and disintegrating.

The invention relates to the technical field of medical equipment, and discloses a puncture device for bone marrow treatment in the hematology department. The device comprises a pipe body, a needle head and a bone marrow fluid box; three groups of inclined telescopic brackets are fixedly mounted on the lower surface of a mounting ring at equal intervals, a group of piston cavities are formed in the inner top of the pipe body, the bottom of each piston cavity communicates with a telescopic pipe arranged on the upper side of the middle in the pipe body, the bottom of the telescopic pipe communicates with a needle pipe, the bottom end of the needle pipe penetrates through the lower side wall of the pipe body and outwards communicates with the needle head, the middle of the needle pipe is fixedly sleeved with a set of sleeve blocks, sliding blocks are fixedly installed on the right sides of the sleeve blocks through threaded rods, and the lower portion of the right side wall of the telescopic pipe rightwards communicates with an infusion pipe extending out of the pipe body; and a group of disinfection sponge with a circular ring-shaped structure is arranged in the pipe body positionedat the middle lower part of the needle pipe, and a vertical sliding opening is formed in the right side of the disinfection sponge. The device has the advantages of simple operation, high stability, low use cost, and convenient cleaning and disinfection.

The invention discloses a preparation method of bone marrow mesenchymal stem cells, and relates to the field of stem cell preparation. The method comprises the following steps: mixing marrow fluid with 0.9% normal saline, carrying out density gradient centrifugation, and collecting mesenchymal stem cells from the lower layer of plasma; culturing leucoderma cells by using a cell culture plate coated with a CD73 antibody; when the cells grow to 70-80%, digesting the cells with stem cell mild digestive enzymes; continuously culturing the bone marrow mesenchymal stem cells by using a cell cultureplate coated with a CD90 antibody; and when the cells grow to 70-80%, digesting the cells by using the stem cell mild digestive enzymes, and collecting high-purity bone marrow mesenchymal stem cells.According to the invention, the high-purity mesenchymal stem cells are separated from the marrow fluid by combining gradient centrifugation adherence with two rounds of antibody forward screening, a large number of miscellaneous cells do not exist in the culture process, the cells combined with the antibody cannot be eluted into the cells, and the cell function is not affected. The preparation method disclosed by the invention is safe, and the capability of purifying and amplifying the bone marrow mesenchymal stem cells is strong.

Disclosed is a computer-implemented method of analyzing bone marrow fluid, including counting the number of nucleated cells and the number of lipid particles in a bone marrow fluid; and obtaining an index related to bone marrow nucleated cell density, on the basis of the number of nucleated cells and the number of lipid particles.

The invention belongs to the technical field of bone marrow fluid extraction devices, and particularly relates to a rapid quantitative bone marrow fluid extraction device for the hematology department. The device comprises a bone marrow puncture needle main body; the upper end of the bone marrow puncture needle main body is clamped and connected with a bidirectional jacket, and the upper end of the bidirectional jacket is clamped and connected with a fixed clamping sleeve for rapidly replacing a needle core; a suction needle used for extracting marrow fluid of a patient is arranged in the fixing clamping sleeve, and a rubber sleeve used for preventing the suction needle from sliding is arranged on the outer side of the suction needle in a sleeving mode. Through the bidirectional jacket arranged at the upper end of the bone marrow puncture needle main body, the bone marrow puncture needle main body is more stable during puncturing, and the working efficiency is improved; the replacement speed of the needle core is increased through the fixed clamping sleeve connected to the upper end of the bone marrow puncture needle body in a clamped mode; through the rubber sleeve arranged at the upper end of the suction needle in a sleeving mode, the needle core is stable and not prone to sliding, and suction work is smoother; and through the screw arranged in the transparent graduated needle cylinder, extraction is quantitative, and waste is not prone to occurring.

The invention discloses a new method for cryopreserving hematopoietic stem cells. The new method comprises the following steps: step 1, taking a hematopoietic stem cell protection agent, a hematopoietic stem cell suspension solution or bone marrow fluid at the volume ratio of (1-2) to 6; step 2, uniformly mixing the hematopoietic stem cell suspension solution or the bone marrow fluid with the hematopoietic stem cell protection agent; emptying air and sealing, and storing the mixed solution in a refrigerator in a sealed manner at -80 DEG C for 12 hours; and step 3, taking out the mixed solution of the hematopoietic stem cell suspension solution or the bone marrow fluid and the hematopoietic stem cell protection agent; and then adding the hematopoietic stem cell protection agent, wherein the volume ratio of the hematopoietic stem cell protection agent to the mixed solution is 1 to 3; and emptying air and sealing again, and storing the mixed solution in the refrigerator in a sealed manner at -80 DEG C. The cryopreserving method provided by the invention is simple and convenient, is easy to operate and has a relatively good cryopreserving effect.

The invention provides a Wright-Giemsa staining reagent and an application method thereof. According to a technical scheme in the invention, the staining reagent is characterized in the aspect of a preparation method; the preparation method is simple and convenient and can be implemented in a short time; and the obtained staining reagent has long service life in a specific preservation environment. On such a basis, a method for staining a biomaterial smear with the staining reagent is novelly designed; and the method comprises the following steps: staining the smear with 0.25 mL of the staining reagent for 30 s, then dropwise adding 1 mL of a phosphate buffer, maintaining the staining reagent in the phosphate buffer for 20 min, carrying out washing with water and then carrying out drying in the air so as to complete staining. The staining reagent of the invention is simple to prepare, short in staining time, excellent in effect, capable of realizing easy identification of various cells, and mainly applicable to staining detection of specimen smears of bone marrow fluid cells, peripheral blood cells, hydrothorax and ascite cells, cerebrospinal fluid cells and the like.

The invention belongs to the technical field of medical equipment, and discloses a bone marrow collecting filtering device and method. The device is provided with an injector with a hole, a connectinghole and a filtering tube; the bone marrow collecting function can be achieved, the injector with the hole is connected with a puncture needle, and the bone marrow is extracted; compared with a traditional injector with a hole, the connecting hole connected with the outside is additionally formed in the injector with the hole and can be connected with the filtering tube for filtering and conveying the bone marrow in the sealing state; a first filtering screen with the bore diameter of 300 microns can filter out small bone marrow particles composed of tissue cells and bone and skin chippings to avoid the blockage of a catheter; a second filtering screen with the bore diameter of 100 microns can filter out fat droplets, hematopoiesis islet cell clusters and the like to avoid that after thebone marrow is injected in the human body, embolism and other complications can be easily caused; a flowmeter can monitor the flow speed; a single chip microcomputer can analyze changes of the flow speed to determine whether or not impurities are contained in the bone marrow liquid; a blood pump provides power to make the collected bone marrow flow in the device, and the efficiency of the filtering process is improved.

The present invention discloses a combined push type bone marrow extractor. The combined push type bone marrow extractor comprises a cylindrical outer shell, an anesthetic needle, a marrow extractingneedle and a puncture needle, one end of the cylindrical outer shell is provided with a sealing bottom plate, the other end of the cylindrical outer shell is movably provided with a rotating cover, the anesthetic needle, the marrow extracting needle and the puncture needle are all movably inserted in the rotating cover, a needle hole is arranged in the sealing bottom plate, besides, an inner sideof the needle hole is provided with a fixed hole, the puncture needle comprises a needle cylinder and a push-pull rod, a sliding hole is arranged in a front end of the needle cylinder, the sliding hole is internally movably provided with a fixed sleeve in a clamping manner, one end of the fixed sleeve is fixedly connected with a puncture needle head, one end of the push-pull rod is movably inserted in the sliding hole, and the other end of the push-pull rod is fixedly connected with a push-pull plate. Only one-time puncture is needed to finish operations of anesthetic injection and marrow extracting, puncture times are reduced and pains of patients caused by puncture are reduced; and a positioning structure is arranged on the surface of the device, can fix the device during the puncture and marrow extracting, conveniently completes the whole puncture and marrow fluid extraction work, at the same time can prevent needle head vibration during the puncture and marrow extracting, and the pains of the patients are avoided.

The invention belongs to the field of tumor molecular diagnosis, and particularly relates to application of fluorescence in-situ hybridization on bone marrow smears in multiple myeloma. The application specifically comprises the following steps: making a bone marrow fluid into bone marrow smears for Wright's staining, and selecting an area with more myeloma cells and good dispersion as an FISH (fluorescence in-situ hybridization) area; covering the FISH area with a fresh stationary liquid to fade the FISH area and fix the cells, washing the bone marrow smears in a 2*SSC solution of 56 DEG C for 10 min, rapidly rinsing the bone marrow smears in deionized water once, and then putting the bone marrow smears into gradient ethanol for dehydration and air-drying; adding a FISH probe into the FISH area for hybridization, dyeing by using a DAPI counterstaining agent, and carrying out microscopic examination on a fluorescence hybridization signal. The bone marrow smear FISH detection method isespecially suitable for the condition that the proportion of monoclonal abnormal plasma cells in flow immunotyping is lower than 20%, and the proportion of myeloma cells in bone marrow smear Wright'sstaining morphology is higher than 20%.

Differentiation of mesodermal stem cells into nervous system cells can be induced by immortalizing the mesodermal stem cells by overexpressing or activating an immortalization gene therein and then culturing these cells under appropriate conditions. This method is highly useful in nerve regeneration therapy.

Provided are a method for assisting the determination of hematological stage of childhood acute lymphoblastic leukemia (childhood ALL) and an extracorporeal diagnostic agent usable in the method. Themethod for assisting the determination of hematological stage of childhood ALL comprises: (1) a step for acquiring the mRNA quantity of Wilms' tumor gene 1 (WT1) in biological sample(s) comprising theperipheral blood and / or the bone marrow fluid of a subject; (2) a step for acquiring the mRNA quantity of GAPDH in the above sample(s); and (3) a step for calculating an index value necessary for assisting the determination on the basis of the ratio between the WT1 mRNA quantity and the GAPDH mRNA quantity that are acquired respectively in the above steps (1) and (2).

The invention discloses a bone marrow fluid rapid and quantitative extraction device for the hematology department. The device comprises a sleeve rod, wherein the left side of the sleeve rod communicates with a liquid outlet pipe, and the left side of the liquid outlet pipe is in threaded connection with a threaded connector. The left side of the threaded connector communicates with a connecting hose, and the left side of the connecting hose communicates with a puncture needle. A driving frame is fixedly connected to the right side of the sleeve rod, and an electric telescopic rod is fixedly connected to the right side of an inner cavity of the driving frame. Through the sleeve rod, the puncture needle, the driving frame, screw rods, a driving disc, a transparent observation window, a rubber stop block, a moving rod, a piston, a screw block, a moving plate and the electric telescopic rod of the invention, the quantitative extraction function of the device can be achieved. The problemsthat an existing marrow fluid extraction device on the market does not have the quantitative extraction function, quantitative extraction cannot be achieved in the bone marrow extraction process in the using process, and the extraction amount is difficult to control for staffs, thereby not being beneficial to disease analysis and treatment are solved.

The invention relates to a collection device and collection method for mesenchymal stem cells. The collection device for mesenchymal stem cells includes a liquid storage apparatus and a material storage apparatus that are communicated with each other, the liquid storage apparatus is used for accommodating blood and / or bone marrow fluid, the material storage apparatus is used for accommodating porous bioceramics. The blood and / or bone marrow fluid flows between the liquid storage apparatus and the material storage apparatus to and fro, and are / is filtered by the porous bioceramics in the material storage apparatus, so that mesenchymal stem cells in blood and / or bone marrow fluid remain on the porous bioceramics. The collection device and collection method provided by the invention are carried out in an aseptic environment, can avoid the probability of chemical and biological pollution, and the compounded product can be used in clinical practice immediately. The method and device provided by the invention can be used in the fields of biology, pharmacy, medicine and veterinary medicine, etc.

The invention discloses a centralized collection device for the marrow fluid sample of a blood disease, and belongs to the technical field of medical instruments. The invention discloses a centralized collection device for the marrow fluid sample of a blood disease. The centralized collection device comprises a positioning disc, wherein a plurality of through holes are arranged on the surface of the positioning disc; a cylinder is rotationally connected to the middle of a limiting block; a limiting sliding groove rod is arranged at the lower end of the inner side of the limiting block; a recoverer body is inserted into the middle of a moving block; and an iodine cotton block is arranged on the lower side of the moving block. Due to tight cooperation among structures, when the centralized collection device is used, after the positioning disc is fixed, a medical worker observes a camera for carrying out an operation, so that the moving block can rotate and move inwards and outwards, coordinate positioning is conducted on a to-be-punctured part, after disinfection is conducted through the iodine cotton block, the recoverer body is inserted into the moving block for carrying out puncture sampling, accurate positioning can be achieved, meanwhile, the situation that the medical worker holds with the hand and shakes during puncture and extraction through holding with the hand is avoided, the comfort level of a patient is improved, and puncture and extraction effects and efficiency are improved.

The invention belongs to the technical field of medical equipment, and discloses a bone marrow collecting and filtering device and method; it is provided with: a syringe with a hole, a connecting hole, and a filter tube; it can realize the function of collecting bone marrow, and the head of the syringe with a hole is connected to a puncture needle, Bone marrow is extracted; compared with the traditional syringe, the perforated syringe has one more connection hole to connect to the outside, which can be connected to a filter tube for the bone marrow to be filtered and transported in a closed state; the first filter screen with a pore size of 300 microns can filter out The bone marrow granules composed of fibroblasts, as well as bone and skin debris, can prevent the catheter from being blocked; the second filter with a pore size of 100 microns can filter out fat droplets, hematopoietic island cell clusters, etc., and prevent embolism after transfusion into the patient's body. Complications; the flow rate meter can monitor the flow rate; the single-chip microcomputer can analyze the change of the flow rate and determine whether there are still impurities in the bone marrow fluid; the blood pump provides power to make the collected bone marrow flow in the device to speed up the efficiency of the filtration process.

The invention relates to a bone marrow aspiration extraction device with a positioning frame. The device comprises a bone marrow aspiration extraction needle and an aspiration positioning frame for auxiliary positioning in an aspiration process, A bone marrow aspiration needle includes a cylindrical housing, a aspiration needle, needle core assembly, Anesthesia needle and pulp withdrawal needle, alancet core assembly include a lancet core body and a lancet control handle, an anesthetic needle comprise an anesthetic syringe, a piston and a push-pull rod arranged in the anesthetic syringe. Thepulp extracting needle comprises a pulp extracting needle barrel, a piston arranged in the pulp extracting needle barrel and a push-pull rod; the anesthetic needle barrel and the pulp extracting needle barrel are respectively passed through the other two channels of the rotary cover; and the aspiration positioning frame comprises a rectangular frame, a positioning block, an aspiration block, a longitudinal positioning strip, a transverse positioning strip and four legs. The bone marrow aspiration needle not only can solve the problem of reducing the aspiration times, but also is provided witha positioning frame, which is convenient for medical personnel to carry out aspiration and bone marrow fluid extraction operation.

The invention discloses a gelatin sponge material containing autologous bone marrow stem cells for repairing intervertebral disc injury and an application method thereof. The gelatin sponge material containing autologous bone marrow stem cells is prepared from autologous bone marrow stem cells and gelatin sponge particles, wherein the autologous bone marrow stem cells are adsorbed onto or into thegelatin sponge particles through the physical mesh structure and surface chemisorption thereof. The application method of the gelatin sponge material containing autologous bone marrow stem cells comprises the following steps: a, extracting bone marrow blood, putting the gelatin sponge particles into a negative pressure enricher for bone growth, injecting extracted bone marrow fluid, starting a negative pressure enrichment process, and repeating an enrichment cycle; b, implanting the gelatin sponge particles on which the bone marrow stem cells are enriched obtained in the step a into nucleus pulposus defects, and meanwhile suturing fibrous ring breaches. The gelatin sponge material can be applied to animal experiments of sheep, pigs, monkeys and the like. Meanwhile, the gelatin sponge material can also be used for repairing simple nucleus pulposus, simple fibrous rings and fibrous ring-nucleus pulposus simultaneously.

The invention discloses an isolated culture method of leukemia variant cells, and belongs to the technical field of cell culture. The method comprises the following steps of: (1) collecting marrow fluid; (2) separating; (3) preparing a cell suspension; and (4) culturing. The invention provides an isolated culture method of leukemia variant cells. The cells isolated and cultured by the method have high cell survival rate. The cell state is the best in 24 hours. Although part of cells die in 48-72 hours, the difference has no statistical significance. The isolated cells are similar to in-vivo cells in biological characters and stable in genetic character. A good cell source is provided for leukemia biology, cytogenetics, in-vitro induced differentiation and the like.

The invention relates to the technical field of bone remodeling, in particular to a method for researching alcoholic bone remodeling based on marrow fluid metabonomics. The bone remodeling steps comprise: Step 1, performing CT (Computed Tomography) on a leg of a patient suffering from a malignant tumor so as to determine the range of the tumor attached to the bone; Step 2, enabling the patient to lie flat on a sickbed, then splitting the tumor part of the leg of the patient, and next cutting off the bone with the tumor part through cutting-off equipment; and Step 3, drilling positioning holes in outer sides of two sections of the cut bone through hole drilling equipment. By using the method, the cut-off bone is subjected to soaking tumor removing treatment, and is then reset again; through cooperation with autonomous metabolism of marrow fluid and auxiliary supporting extrusion of a positioning mechanism, the bone remodeling effect is guaranteed, and the bone remodeling period is also shortened; an additional supporting mechanism does not need to be additionally added to the remodeling part, so that a repulsion phenomenon is avoided; the self repair of bone remodeling is well realized; and the reliability of bone remodeling is greatly improved.

The invention discloses a marrow fluid cell partition method based on depth study, and relates to the field of biological medical image treatment and computer application. Compared with the prior art, the marrow fluid cell partition method provided by the embodiment is realized by a clustering algorithm and a depth study method; the method is direct and easy to practice; through an automatic evaluation system of the partitioning quality, the partitioning result is more exact; the component characteristic of the HSV image is fully used, and the unique regional growth method is designed, and the calculation process is greatly simplified; the efficiency is improved.

The present invention relates to a synapse formation accelerant and a brain plasticity accelerant that contain CD24-negative mesenchymal stem cells prepared from a patient's own bone marrow fluid, aswell as to treatment of dementia, chronic cerebral infarctions, chronic spinal cord injuries, mental disorders, or the like using the aforementioned synapse formation accelerant and brain plasticity accelerant.

Provided are a method for assisting in determining the hematological stage of childhood acute lymphoblastic leukemia (child ALL), and an in-vitro diagnostic pharmaceutical product usable in the method. The method for assisting in determining the hematological stage of child ALL comprises the steps of (1) obtaining the mRNA level of Wilms' tumor-1 gene (WT1) in at least one of the biological samples of peripheral blood and bone marrow fluid from a test subject; (2) obtaining the GAPDH mRNA level in the biological sample; and (3) calculating an index value necessary for assisting in the determination based on the ratio of the WT1 mRNA level obtained in step (1) to the GAPDH mRNA level obtained in step (2).