8 results about "Bone marrow fluid" patented technology

The invention belongs to the field of immunotoxicology, and particularly relates to a method for immunotyping bone marrow cells by flow cytometry. The flow cytometry immune typing method for bone marrow cells comprises the following steps: collecting macaca fascicularis bone marrow fluid, and preparing the macaca fascicularis bone marrow fluid into single-cell suspension; dyeing the obtained cells by using an antibody combination containing a plurality of fluorescent labels; then, a flow cytometer is used for collecting cell fluorescence signals, a standardized gate setting strategy is executed, and based on collected data, the percentage of each cell subset in CD45 + living white blood cells is calculated to complete immune typing. According to the flow cytometry immune typing method for the bone marrow cells, the problems of low efficiency, low repeatability, low sensitivity and poor specificity of related technologies are solved, and more accurate and reliable data support is provided for early discovery, risk assessment and mechanism research of drug immunotoxicity.

The utility model relates to the technical field of metacarpal bone medical treatment, and discloses a metacarpal bone absorbable intramedullary needle which comprises a main nail and a matching nail, the interior of the main nail is designed to be of a hollow threaded structure, the main nail and the matching nail are used in a paired and matched mode, and the main nail and the matching nail are both made of PLGA materials. The matching nails comprise the first matching nail, the second matching nail and the third matching nail, and the first matching nail, the second matching nail and the third matching nail can be sequentially and rotationally placed into the main nail. According to the utility model, the main nail and the matching nail are both made of the PLGA material, and the PLGA material has good biocompatibility, no toxicity and good capsulation and film forming performance, so that good stability can be provided, marrow fluid in metacarpal bone can be absorbed, a secondary extraction operation is avoided, the previous two operations are changed into one operation, and the operation efficiency is improved. And meanwhile, the treatment cost can be reduced, and the defects that the metal intramedullary needle needs to be taken out for the second time and the treatment cost is increased in the current domestic and international treatment schemes are overcome.

The invention discloses a blood disease marrow fluid sample collection device, and belongs to the technical field of medical instruments, the device comprises a collection main body, a first piston assembly, a second piston assembly and a filter assembly, the collection main body is internally provided with a first chamber; the first piston assembly is arranged in the first cavity, the second cavity is formed in a piston rod body of the first piston assembly, the second piston assembly is arranged in the second cavity, and the second cavity and the first cavity are connected through a second cavity communicating channel; the filtering assembly is arranged at the front end of the communicating channel of the second cavity, after the first piston assembly is driven to extract marrow fluid into the first cavity, the collecting body is removed from the puncture needle, and the second piston assembly is driven to suck the marrow fluid in the first cavity into the second cavity; the bone marrow particle tissue in the bone marrow fluid in the first cavity is collected through the filter screen, separated collection of the bone marrow fluid and the bone marrow particles is completed, the complete structure of the bone marrow particles can be guaranteed, and the collection efficiency is higher.

The closed type hydraulic piston marrow fluid collector comprises a collecting pipe, a fluid pumping mechanism, a second one-way valve and a pumping-out connecting thin pipe, and the collecting pipe comprises a pipe body, a pipe opening wing, a first one-way valve and a pumping-in connecting thin pipe; a pipe opening wing is arranged at the top of the pipe body, a first one-way valve is arranged at the inner bottom of the pipe body, and the bottom of the first one-way valve is connected with a pumping-in connecting thin pipe. Closed one-way hydraulic piston pump type bone marrow fluid collection is achieved through the design of the two one-way valves, the problems of non-pressure reciprocating low-efficiency bone marrow fluid suction and non-closed type bone marrow fluid collection of a traditional injector are solved, it is avoided that medical staff repeatedly pull out and connect the injector, time and labor are saved, and the working efficiency is improved. The risk that the bone marrow fluid is polluted by pathogenic microorganisms is reduced; the connecting slim tube which is provided with the plastic needle head and used for pumping out marrow fluid is designed, the connecting slim tube can be directly punctured into a marrow fluid collecting tube, single-person sterile operation of medical staff is facilitated, and the defect that multiple persons need to operate when a traditional syringe injects marrow fluid is overcome.

The bone marrow puncture extraction device comprises an extraction assembly, the bottom end of the extraction assembly is connected with a limiting sleeve, rectangular grooves are symmetrically formed in the two sides of the limiting sleeve, positioning plates are arranged in the rectangular grooves of the limiting sleeve, the positioning plates are in sliding connection with a finger plate, and the finger plate is connected with the limiting sleeve; and the bottom end of the fingerboard is provided with a groove which is connected with the supporting rod in a clamping manner. According to the bone marrow puncture extraction device for the leukemia patient in the hematology department, the butt joint pipe at one end of the extraction pipe is directly in threaded connection with the connecting pipe to form a bone marrow puncture extraction structure, and the limiting ring arranged in the butt joint pipe is in linkage with the anti-overflow block to form a corresponding non-return structure; the limiting ring and the spill-proof block are separated and opened, the puncture needle is inserted into the body of a patient, marrow fluid penetrates through the limiting ring and the spill-proof block and flows into the extraction tube, and after extraction is completed, the limiting ring and the spill-proof block are closed to form a sampling tube.

The application discloses a bone marrow cavity targeted drug release microsphere and belongs to the technical field of medical devices. The drug release microsphere is spherical in structure and contains drugs, and can be implanted into the inside of a bone marrow cavity. The spherical structure is suitable for the microenvironment of the bone marrow cavity, and after implantation, the drug release microsphere is freely distributed, does not press and does not damage tissues, fully contacts with bone marrow fluid, guarantees the global uniform dispersion of drugs, locally releases drugs for a long time, maintains the effective drug concentration in the bone marrow cavity, has remarkable treatment effect, and rarely enters the whole body circulation, greatly reduces the side effects of drugs on the whole body, is minimally invasively implanted, is simple to operate, has small trauma, is fast in postoperative recovery, reduces the treatment cost and physical burden of patients, is preferably made of a degradable medical biocompatible material, has no immune rejection, can be synchronously degraded and has no residue, does not need secondary surgery, and is high in clinical safety. The drug release microsphere can be flexibly designed in specification, drug type and loading mode, is wide in clinical adaptability, can be used alone or in combination with other devices, and is rich in application scenarios.

A tissue engineering cartilage constructed based on a decalcified bone scaffold, a preparation method therefor, and use thereof in the repairing of joint defects are provided. A marrow fluid of a patient is extracted, bone marrow mesenchymal stem cells of the patient are isolated and amplified in vitro, and the bone marrow mesenchymal stem cells isolated and cultured in vitro are inoculated into a decalcified bone scaffold, thus constructing a tissue engineering cartilage in vitro. The tissue engineering cartilage can be transplanted to a joint defect part, so that the effective repair of joint defects and function reconstruction are realized.

The application belongs to the technical field of bone marrow puncture, and relates to a bone marrow puncture post-aspiration device, which comprises a mounting shell, a transmission box fixedly arranged on the mounting shell and a limiting arc plate rotatably arranged on the mounting shell. The limiting block in the limiting unit is used for accurately limiting the liquid aspiration stroke, and the rack and pinion transmission quantized volume is used for ensuring the accurate aspiration amount and avoiding the excessive risk. Through the damping effect of the hydraulic damper, the moving speed of the liquid aspiration slider is limited, the aspiration speed is controllable, and the pain and tissue damage of the patient are reduced. With the bidirectional locking structure of the ratchet and the pawl, the reverse movement of the liquid aspiration slider is prevented, the bone marrow liquid backflow is prevented, and the infection and hematoma risk are reduced. The disposable syringe and the hydraulic damper are detachably designed through detachable clamping, disinfection is facilitated, and cross infection is reduced. The hydraulic transmission unlocking and the liquid outlet knob are one-key operated, the operation safety and convenience are improved, and the clinical precise and safe aspiration requirement is met.