22 results about "Bone marrow fluid" patented technology

The invention belongs to the technical field of biological genetic engineering, and relates to a high-risk myelodysplastic syndrome screening gene and a diagnostic kit thereof. The invention provides an application of detecting YTHDF2, METTL3 and NAT10 gene expression of bone marrow cells of high-risk MDS patients as a specific molecular marker for screening the high-risk myelodysplastic syndrome, and by detecting mRNA expression levels of the YTHDF2, METTL3 and NAT10 genes, the screening of the patients in the high-risk myelodysplastic syndrome is facilitated. Meanwhile, treatment and prognosis evaluation of patients with high-risk myelodysplastic syndromes can be better guided. Only the bone marrow fluid of the patient needs to be extracted and collected during bone marrow cytology examination of the patient, the patient compliance is good, the bone marrow fluid is selected from human bone marrow fluid mononuclear cells, the detection result is more accurate, and the method is suitable for clinical large-scale application.

The invention provides a biomarker, a model and a system for prognosis evaluation of acute myelogenous leukemia and application of the biomarker, the model and the system. According to the invention, a prognosis model for evaluating the risk of acute myelogenous leukemia is constructed by using a biomarker consisting of GZMB, OPTN, DNM1, MPO and GZMH genes. The method comprises the following steps: extracting marrow fluid nucleated cell RNA of a patient with acute myelogenous leukemia, detecting the relative expression quantity of the five genes in a quantitative detection manner, substituting into a constructed risk prediction model, and performing risk prognosis evaluation according to scores. The method is low in detection cost, low in technical threshold and better in prediction performance, and has independent prognosis evaluation value.

The present invention belongs to the field of biological genetic engineering technology and relates to high-risk myelodysplastic syndrome screening genes and diagnostic kits. The present invention provides an application of detecting the expression of YTHDF2, METTL3, and NAT10 genes in bone marrow cells of high-risk MDS patients as a specific molecular marker for screening high-risk myelodysplastic syndrome. By detecting the expression levels of YTHDF2, METTL3, and NAT10 genes mRNA, it helps to screen patients with high-risk myelodysplastic syndrome and better guide the treatment and prognosis evaluation of patients with high-risk myelodysplastic syndrome. The present invention only needs to extract the patient's bone marrow fluid and collect it together when the patient undergoes bone marrow cytology examination. The patient compliance is good, and the material is taken from human bone marrow fluid mononuclear cells. The test results are more accurate and suitable for large-scale clinical application.

The invention discloses a high-precision detection method of abnormal plasma cells, which comprises the following steps: preparing a film, immediately and uniformly mixing the extracted marrow fluid with an anticoagulant, sequentially diluting and centrifuging the marrow, then collecting a leukocyte layer, washing with a fluid medium RPMI 1640 / 5% FBS for at least one time, then counting the cells, adjusting the cell concentration to 1 * 10 < 6 > / ml, adding 100 [mu] l of the cell suspension into a funnel of a cell centrifugal smear device, centrifuging at room temperature for 1000 revolutions, continuing for 5 minutes, and preserving a slide filled with the cell suspension; the preparation of FISH cells comprises the following steps: firstly, preparing and pretreating a 90% formamide / 2 * SSC solution; according to the method, the accuracy and the efficiency of abnormal plasma cell detection are improved by combining an intelligent AI recognition model and a fluorescence in situ hybridization (FISH) technology. The application of the intelligent AI recognition model can reduce personal errors and improve the recognition capability of fluorescence signals, thereby improving the accuracy of detection results.

The invention belongs to the field of immunotoxicology, and particularly relates to a method for immunotyping bone marrow cells by flow cytometry. The flow cytometry immune typing method for bone marrow cells comprises the following steps: collecting macaca fascicularis bone marrow fluid, and preparing the macaca fascicularis bone marrow fluid into single-cell suspension; dyeing the obtained cells by using an antibody combination containing a plurality of fluorescent labels; then, a flow cytometer is used for collecting cell fluorescence signals, a standardized gate setting strategy is executed, and based on collected data, the percentage of each cell subset in CD45 + living white blood cells is calculated to complete immune typing. According to the flow cytometry immune typing method for the bone marrow cells, the problems of low efficiency, low repeatability, low sensitivity and poor specificity of related technologies are solved, and more accurate and reliable data support is provided for early discovery, risk assessment and mechanism research of drug immunotoxicity.

The utility model relates to the technical field of bone sawing machines, discloses a meat bone sawing machine, and solves the problems that liquid such as bone residues, blood and marrow liquid after bone sawing accumulates at the top end of a working table, the table top of the working table is polluted, and the risk of cross contamination is increased. A meat bone sawing machine comprises a workbench, supporting legs, a supporting plate, a top plate, a cutting and sawing assembly, a protection box and a waste collecting assembly, impurities such as blood and bone residues generated during cutting fall into a filtering drawer along through holes, particle impurities such as the bone residues are blocked by filtering holes under the action of the filtering holes, and liquid such as the blood flows into the waste collecting drawer along the filtering holes. The filtering drawer and the waste collecting drawer can be easily taken out through the first sliding groove and the second sliding groove, impurities such as blood and bone residues are directly collected into the waste collecting box, residues of the impurities at the top end of the workbench are reduced, and the risk of cross contamination is reduced.

The utility model relates to the technical field of bone marrow fluid extraction, and discloses a special injector for bone marrow fluid extraction, which is characterized in that an annular bulge is arranged at a proper position in a cylinder body, so that when a rubber plug moves to be propped against the annular bulge, the amount of extracted bone marrow fluid in the cylinder body behind the rubber plug reaches a preset value; the situation that the bone marrow fluid is diluted due to too much suction amount is avoided; meanwhile, through the cooperation of a first one-way conduction piece and a second one-way conduction piece, after the preset bone marrow fluid extraction amount is reached, the extracted bone marrow fluid can be discharged through a discharge pipe only by pushing the extraction part in the reverse direction, and the bone marrow fluid is used for smearing the bone marrow fluid; then, the extraction part is controlled to continue to move, subsequent bone marrow fluid extraction is carried out, in the whole process, the syringe body does not need to be detached, the operation time is shortened, and the probability of operation failure caused by unmatched struggling of children patients can be reduced; in the bone marrow fluid extraction process, the injector and the puncture needle do not need to be separated, and the subsequently extracted bone marrow fluid sample is prevented from being polluted.

The utility model relates to the technical field of metacarpal bone medical treatment, and discloses a metacarpal bone absorbable intramedullary needle which comprises a main nail and a matching nail, the interior of the main nail is designed to be of a hollow threaded structure, the main nail and the matching nail are used in a paired and matched mode, and the main nail and the matching nail are both made of PLGA materials. The matching nails comprise the first matching nail, the second matching nail and the third matching nail, and the first matching nail, the second matching nail and the third matching nail can be sequentially and rotationally placed into the main nail. According to the utility model, the main nail and the matching nail are both made of the PLGA material, and the PLGA material has good biocompatibility, no toxicity and good capsulation and film forming performance, so that good stability can be provided, marrow fluid in metacarpal bone can be absorbed, a secondary extraction operation is avoided, the previous two operations are changed into one operation, and the operation efficiency is improved. And meanwhile, the treatment cost can be reduced, and the defects that the metal intramedullary needle needs to be taken out for the second time and the treatment cost is increased in the current domestic and international treatment schemes are overcome.

The present invention relates to a bone marrow fluid analysis method, a sample analysis device, and a recording medium containing a program. The present invention provides a means for obtaining an index related to bone marrow nucleated cell density by measuring nucleated cells and lipid particles in bone marrow fluid. By counting the number of nucleated cells and lipid particles in bone marrow fluid and obtaining an index related to bone marrow nucleated cell density based on the nucleated cell and lipid particle counts, the aforementioned technical problems are solved.

The invention discloses a blood disease marrow fluid sample collection device, and belongs to the technical field of medical instruments, the device comprises a collection main body, a first piston assembly, a second piston assembly and a filter assembly, the collection main body is internally provided with a first chamber; the first piston assembly is arranged in the first cavity, the second cavity is formed in a piston rod body of the first piston assembly, the second piston assembly is arranged in the second cavity, and the second cavity and the first cavity are connected through a second cavity communicating channel; the filtering assembly is arranged at the front end of the communicating channel of the second cavity, after the first piston assembly is driven to extract marrow fluid into the first cavity, the collecting body is removed from the puncture needle, and the second piston assembly is driven to suck the marrow fluid in the first cavity into the second cavity; the bone marrow particle tissue in the bone marrow fluid in the first cavity is collected through the filter screen, separated collection of the bone marrow fluid and the bone marrow particles is completed, the complete structure of the bone marrow particles can be guaranteed, and the collection efficiency is higher.

Disclosed is a computer-implemented method of analyzing bone marrow fluid, including counting the number of nucleated cells and the number of lipid particles in a bone marrow fluid; and obtaining an index related to bone marrow nucleated cell density, on the basis of the number of nucleated cells and the number of lipid particles.

The utility model relates to the technical field of medical instruments, and discloses a bone marrow puncture extraction device, including a connecting sleeve fixedly connected to the side of a pressure plate, the side of the connecting sleeve is clamped with a positioning block for connection, the side of the positioning block is fixedly connected with a puncture needle, the outer surface of the puncture needle is clamped with a clamping ring for positioning, and the side of the clamping ring is fixedly connected with a positioning sleeve for fixing. The bone marrow puncture extraction device, through the coordinated arrangement of the puncture needle, the clamping ring and the positioning sleeve, can clamp the positioning sleeve on the outer surface of the puncture needle through the clamping ring before the medical staff performs puncture, and after the medical staff inserts the puncture needle into the patient's body and reaches a suitable position, the positioning sleeve is fitted with the patient's skin, which can limit the puncture needle, thereby ensuring that the puncture needle will not twist when the medical staff extracts bone marrow fluid, reducing the tingling sensation.

The closed type hydraulic piston marrow fluid collector comprises a collecting pipe, a fluid pumping mechanism, a second one-way valve and a pumping-out connecting thin pipe, and the collecting pipe comprises a pipe body, a pipe opening wing, a first one-way valve and a pumping-in connecting thin pipe; a pipe opening wing is arranged at the top of the pipe body, a first one-way valve is arranged at the inner bottom of the pipe body, and the bottom of the first one-way valve is connected with a pumping-in connecting thin pipe. Closed one-way hydraulic piston pump type bone marrow fluid collection is achieved through the design of the two one-way valves, the problems of non-pressure reciprocating low-efficiency bone marrow fluid suction and non-closed type bone marrow fluid collection of a traditional injector are solved, it is avoided that medical staff repeatedly pull out and connect the injector, time and labor are saved, and the working efficiency is improved. The risk that the bone marrow fluid is polluted by pathogenic microorganisms is reduced; the connecting slim tube which is provided with the plastic needle head and used for pumping out marrow fluid is designed, the connecting slim tube can be directly punctured into a marrow fluid collecting tube, single-person sterile operation of medical staff is facilitated, and the defect that multiple persons need to operate when a traditional syringe injects marrow fluid is overcome.

The bone marrow puncture extraction device comprises an extraction assembly, the bottom end of the extraction assembly is connected with a limiting sleeve, rectangular grooves are symmetrically formed in the two sides of the limiting sleeve, positioning plates are arranged in the rectangular grooves of the limiting sleeve, the positioning plates are in sliding connection with a finger plate, and the finger plate is connected with the limiting sleeve; and the bottom end of the fingerboard is provided with a groove which is connected with the supporting rod in a clamping manner. According to the bone marrow puncture extraction device for the leukemia patient in the hematology department, the butt joint pipe at one end of the extraction pipe is directly in threaded connection with the connecting pipe to form a bone marrow puncture extraction structure, and the limiting ring arranged in the butt joint pipe is in linkage with the anti-overflow block to form a corresponding non-return structure; the limiting ring and the spill-proof block are separated and opened, the puncture needle is inserted into the body of a patient, marrow fluid penetrates through the limiting ring and the spill-proof block and flows into the extraction tube, and after extraction is completed, the limiting ring and the spill-proof block are closed to form a sampling tube.

The utility model relates to an angle-adjustable bone marrow pushing piece clamping assembly which comprises a bottom plate, and a positioning groove is formed in the bottom plate. A transverse moving support is arranged on the bottom plate in a sliding mode, a height-adjustable adjusting seat is arranged at the upper end of the transverse moving support, a material carrying plate for carrying a bone marrow pushing piece is arranged on the adjusting seat in a rotating mode, and a locking screw and a clamp are arranged on the material carrying plate. An identification plate is fixedly arranged on one side of the adjusting seat; during use, after the angle of the material carrying plate is adjusted according to the identification plate, the bone marrow pushing piece is fixed to the material carrying plate, then a glass slide is placed in the positioning groove, the height position of the material carrying plate is adjusted, then bone marrow liquid is dropped on the glass slide, the whole support is transversely moved, the bone marrow pushing piece is driven to move, and the bone marrow liquid is spread on the glass slide; the inclined angle of the bone marrow pushing piece can be accurately adjusted, the distance between the bone marrow pushing piece and the glass slide is a constant value when the bone marrow pushing piece moves, therefore, a bone marrow membrane sample with the uniform thickness can be obtained, the overall structure is simple, operation is convenient, and cost is low.

The invention provides a chimeric antigen receptor modified CD34 + stem cell as well as a preparation method and application thereof, and belongs to the technical field of stem cell treatment. The preparation method comprises the following steps: step 1, flushing marrow fluid from femoral marrow of a C57BL / 6J mouse, and performing centrifugal separation and purification to obtain purified femoral marrow mononuclear cells of the C57BL / 6J mouse; step 2, separating and purifying the purified C57BL / 6J mouse femoral bone marrow mononuclear cells by a magnetic bead separation method to obtain CD34 + cells, and culturing and proliferating for passage to obtain primary CD34 + stem cells of the C57BL / 6J mouse; step 3, constructing a CAR overexpression plasmid; 4, packaging the CAR overexpression plasmid and the lentivirus to obtain a CAR overexpression lentivirus vector; 5, the CAR overexpression lentiviral vector and C57BL / 6J mouse primary CD34 + stem cells are subjected to mixed culture, and the chimeric antigen receptor modified CD34 + stem cells are obtained.

The application discloses a bone marrow cavity targeted drug release microsphere and belongs to the technical field of medical devices. The drug release microsphere is spherical in structure and contains drugs, and can be implanted into the inside of a bone marrow cavity. The spherical structure is suitable for the microenvironment of the bone marrow cavity, and after implantation, the drug release microsphere is freely distributed, does not press and does not damage tissues, fully contacts with bone marrow fluid, guarantees the global uniform dispersion of drugs, locally releases drugs for a long time, maintains the effective drug concentration in the bone marrow cavity, has remarkable treatment effect, and rarely enters the whole body circulation, greatly reduces the side effects of drugs on the whole body, is minimally invasively implanted, is simple to operate, has small trauma, is fast in postoperative recovery, reduces the treatment cost and physical burden of patients, is preferably made of a degradable medical biocompatible material, has no immune rejection, can be synchronously degraded and has no residue, does not need secondary surgery, and is high in clinical safety. The drug release microsphere can be flexibly designed in specification, drug type and loading mode, is wide in clinical adaptability, can be used alone or in combination with other devices, and is rich in application scenarios.

The invention belongs to the technical field of medical examination, and particularly relates to application of CD45 + CD8 + HLA-DRB5 + T cells in preparation of a kit for auxiliary diagnosis of aplastic anemia (AA) and graft versus host disease (GVHD). The inventor finds that compared with healthy volunteers and other blood disease patients, the expression of CD45 + CD8 + HLA-DRB5 + T cells in peripheral blood or marrow fluid of AA and GVHD patients is remarkably improved for the first time. The method can be used as one of auxiliary diagnosis detection indexes of AA and GVHD. The invention provides a marker CD45 + CD8 + HLA-DRB5 + T cell for AA and GVHD diagnosis, AA and GVHD can be simply and efficiently diagnosed, and the marker has the advantages of being easy to popularize and rapid.

A tissue engineering cartilage constructed based on a decalcified bone scaffold, a preparation method therefor, and use thereof in the repairing of joint defects are provided. A marrow fluid of a patient is extracted, bone marrow mesenchymal stem cells of the patient are isolated and amplified in vitro, and the bone marrow mesenchymal stem cells isolated and cultured in vitro are inoculated into a decalcified bone scaffold, thus constructing a tissue engineering cartilage in vitro. The tissue engineering cartilage can be transplanted to a joint defect part, so that the effective repair of joint defects and function reconstruction are realized.

The invention belongs to the technical field of biology, and particularly relates to a skull bone marrow artificial buffer solution and a preparation method and application thereof. The artificial buffer solution is mainly prepared from the following raw materials: LiCl, NaHCO3, MgCl2, CaCl2, NaNO2, Na2SO4, Na3PO4. 12H2O, NaCl and glucose, and the pH (Potential of Hydrogen) of the artificial buffer solution is 4 to 5. Components of the skull bone marrow artificial buffer solution are consistent with natural components of skull bone marrow fluid, are close to the pH value of normal skull bone marrow fluid, are more suitable for skull bone marrow cell and molecule related experiments, can be used as a solvent of an injection reagent in a skull, contributes to maintaining an internal environment of the skull bone marrow, and can be used for separating bone marrow cells from the skull bone marrow. The artificial buffer solution prepared by the invention is more suitable for being added into a cell culture medium as a basic buffer solution, so that a cell culture environment is close to an in-vivo environment, and the growth of skull bone marrow cells separated in vivo is facilitated. In addition, in-vitro culture of skull stem cells and stem cells in skull bone marrow injection related research is facilitated.

The application belongs to the technical field of bone marrow puncture, and relates to a bone marrow puncture post-aspiration device, which comprises a mounting shell, a transmission box fixedly arranged on the mounting shell and a limiting arc plate rotatably arranged on the mounting shell. The limiting block in the limiting unit is used for accurately limiting the liquid aspiration stroke, and the rack and pinion transmission quantized volume is used for ensuring the accurate aspiration amount and avoiding the excessive risk. Through the damping effect of the hydraulic damper, the moving speed of the liquid aspiration slider is limited, the aspiration speed is controllable, and the pain and tissue damage of the patient are reduced. With the bidirectional locking structure of the ratchet and the pawl, the reverse movement of the liquid aspiration slider is prevented, the bone marrow liquid backflow is prevented, and the infection and hematoma risk are reduced. The disposable syringe and the hydraulic damper are detachably designed through detachable clamping, disinfection is facilitated, and cross infection is reduced. The hydraulic transmission unlocking and the liquid outlet knob are one-key operated, the operation safety and convenience are improved, and the clinical precise and safe aspiration requirement is met.

The invention belongs to the technical field of bone marrow puncture, and relates to a post-bone marrow puncture suction device which comprises a mounting shell, a transmission box fixedly arranged on the mounting shell and a limiting arc plate rotationally arranged on the mounting shell. A limiting block in the limiting unit is used for precisely limiting the liquid pumping stroke, the volume is quantified in cooperation with rack and gear transmission, it is ensured that the pumping amount is precise, and the excessive risk is avoided; through the damping effect of the hydraulic damper, the moving speed of the liquid extraction sliding block is limited, the extraction speed is controllable, and pain and tissue damage of a patient are reduced; by means of a two-way locking structure of the ratchet wheel and the pawl, the liquid extraction sliding block is prevented from moving reversely, marrow fluid is prevented from flowing back, and infection and hematoma risks are reduced; the detachable design of the disposable syringe and the hydraulic damper which are detachably connected in a clamping mode is adopted, disinfection is convenient, and cross infection is reduced; hydraulic transmission unlocking and one-key operation of a liquid outlet button are achieved, the operation safety and convenience are improved, and the clinical precise and safe suction requirements are met.