105results about How to "Promote growth and proliferation" patented technology

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg/L of 6-BA, and 0.5 mg/L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg/L of 6-BA, 0.5 mg/L of NAA, and 60 g/L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg/L of 6-BA, 0.5 mg/L of 2,4-D, and 60 g/L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0 to 50 mg/L of paclobutrazol, and 60 g/L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg/L of 6-BA, 0.5 mg/L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

The present invention relates to the process of promoting haemtococcus pluvialis growth, raising its population density and controlling the synthesis and accumulation of astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, bacteria-free ventilating and oxygen unit, salty and fresh water nutritious liquid compounding and temperature controlling unit, and cleaning and sterilizing unit; altering environment conditions to induce haemtococcus pulvialis to multiply, raise population density and regulating synthesis, accumulation and collecting haemtococcus pluvialis with rich astaxanthin.

The invention discloses a polypeptide composition for facial care. The polypeptide composition comprises the following components in percentage by mass: 0.0001-0.0005% of acetyl hexapeptide-8, 0.01-0.1% of acetyl tetrapeptide-5, 0.02-0.1% of tripeptide-1 copper, 0.0005-0.1% of glutathione, 0.0001-0.0005% of oligopeptide-1, 0.0001-0.0005% of oligopeptide-2, 0.0001-0.0005% of oligopeptide-3, 0.0001-0.0005% of oligopeptide-4, 0.0001-0.0005% of oligopeptide-5, 1.0-5.0% of polyethylene glycol, 84.697-97.9689% of mannitol and 1.0-10.0% of deionized water. The polypeptide composition disclosed by the invention comprises 9 polypeptide active matters, has the advantages of remedying and whitening skin and preventing aging, and is capable of permeating into skin for improving blood circulation of the face, supplying sufficient nutrients and energy to cells and promoting metabolism of the skin, and thus the purposes of remedying and caring skin can be achieved; and a freeze-drying technique is adopted in a production process, so that various active substances can be retained to the maximum extent, original properties of various active substances can be maintained, conversion of active matters can be prevented, and the stability of a product can be improved.

The invention discloses a method for preparing essence of stem cell factor with an anti-aging effect. The method comprises the steps that step 1, human umbilical cord mesenchyma stem cells are prepared and cultured, stem cell culture supernatant is collected, concentrate is obtained after concentration is conducted, and the concentrate is further frozen and dried to prepare the lyophilized powderof the stem cell factor; step 2, the components of, by volume, 0.1-1% of hyaluronic acid, 1-10% of glycerol, 0.1-1% of collagen protein, 0.1-1% of tea polyphenols and the balance water are mixed to obtain solvent; step 3, the solvent is added into the lyophilized powder of the stem cell factor to be thoroughly dissolved to obtain the essence of the stem cell factor. The prepared essence of the stem cell factor comprises multiple kinds of cell active factor components such as epidermal growth factor, vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor and the like,the essence can nourish skin, promote skin repairing and regenerating, resist wrinkles, remove colored patches and the like, and the essence has better application value in the field of medical beauty.

The invention relates to an antibacterial healthcare spunlace fabric, in particular to a preparation method and application of a mint fiber spunlace non-woven mask substrate. The mint fiber spunlace non-woven mask substrate is prepared from, by weight, 10-100% of mint fiber, 0-90% of copper ammonia fiber, 0-90% of pearl fiber, 0-90% of viscose and 0-90% of polyester fiber. The mint fiber spunlacenon-woven mask substrate has the advantages that the developed mint fiber spunlace non-woven mask substrate is soft in touch and good in adsorption, has the water wetting and lightsome skin feeling and has the efficient anti-aging, anti-radiation, anti-bacterial and anti-oxidative effects, and the mask substrate has the functions of preserving moisture, activating cells, supplying oxygen and the like, and achieves the nourishing, nutritional and healthcare effects on the skin.

The invention discloses jujube-flavored white spirit and a brewing process thereof. A preparation method of the liquor comprises the following steps: grinding, steaming and spread-cooling jujube, wheat, sorghum, glutinous rice, corn, rice, pea and rice hull according to certain proportions, uniformly mixing with the addition of yeast powder, sealing and fermenting in pit mud for more than 4 months; beginning to distill after 4 months in a mode of distilling twice every month; ultrasonically processing liquor obtained from distillation firstly, and then storing the liquor together with tourmaline, limonite and medicinal stone in a porcelain jar for more than 6 months, thus completing aging. The liquor overcomes shortcomings in the prior art, and is simple and convenient in operation, short in production cycle, high in liquor yield and good in quality. The jujube-flavored white spirit disclosed by the invention is stable in quality, free from restoring and long in aftertaste, people drinking the white spirit are not easy to become drunk and quick to be sobered up, and the white spirit, after drunk, is free from a problem of headache, free from such harmful material components as methanol, and is capable of relieving pressure on liver and kidney after drinking and effectively relieving damage to body.

The invention discloses a novel mask, comprising the following components in parts by weight: 5-20 parts of ovoplasm, 0-5 parts of hide gelatin, 5-20 parts of honey, 0-10 parts of egg white, 30-40 parts of plant powder, 0-2 parts of pearl powder, 0-5 parts of fruit and vegetable juice and 0-2 parts of additive. The ovoplasm is serous fluid prepared from eggs and/or pupa of animals. Due to use of the mask disclosed by the invention, blemishes and fine wrinkles on the face can be removed, the defects that the skin is dark and the like can be improved, so that the facial skin is white and red, natural luster can be recovered, the softness and elasticity of the skin are protected, the whiteness of the skin is increased; the mask has no any side effect, thus safety and reliability are achieved, the effects of nourishing the healthy skin and promoting metabolism are achieved, whitening, beautifying, muscle growing, skin moisturizing and wrinkle removing can be achieved, and the pores are astringed. The novel mask can be used as a skin care product for long time.

Disclosed is a construction method of sclerotomal cell idiosyncratic transcription factor - 2 gene decorated tissue engineered bone through employing a natural biologically derived material (acellular bone matrix) and seed cells (osteoblast specific transcription factor-2 gene modified mesen chymal stem cells), carrying out extracorporal dynamic culture and restoration for large segment long bone defection. The method can realize the clinic cicatrisation of bone defections.

The invention relates to an injectable implant and a preparation method thereof. The implant is amnion microparticles mixed in a sodium chloride phosphate physiological solution with a pH of 7.0-7.5. The preparation method comprises the following steps of disinfection processing, decellularization processing, modification processing, and amnion microparticle preparation, and in particular, comprises: taking amnion microparticles as a component A and a sodium chloride phosphate physiological solution as a component B, performing vibration and well mixing according to a mass volume ratio of 20:1-100:1 to prepare an amnion microparticle suspension with a concentration of 20-100 mg/mL by high-speed microjet equipment, wherein the particle size of the amnion microparticles is 50-200 microns. The injectable implant of the invention maintains the natural three dimensional stereo structure and a lot of natural active components of human amnion, reduces immunological rejection reaction caused by raw material source after clinical application of cosmetic injection products, is suitable for injection into deep subcutaneous dermal layer to subcutaneous superficial layer to repair moderate to severe wrinkles or folds, and has good cosmetic repair effect.

The invention discloses a double-chamber three-dimensional pouring bioreactor system which comprises a bioreactor, wherein the bioreactor is internally provided with a three-dimensional bracket and is connected with a pouring device. The bioreactor comprises a first chamber filled with common nutritional nutrient liquor and a second chamber filled with inductive culture factors. A dialysis membrane assembly through which components having molecular weight being less than the molecular weight of inductive culture factors in the inductive culture factors can pass is arranged between the first chamber and the second chamber. The three-dimensional bracket is located in the second chamber. According to the double-chamber structural design of the bioreactor disclosed by the invention, the common nutritional nutrient liquor and the inductive culture factors in the two chambers are independently exchanged, and the inductive culture factors can be preserved while the common nutritional nutrient liquor is changed, so that the use level of the inductive culture factor is greatly reduced, the effective utilization ratio of the inductive culture factors is improved, and the cost is lowered.

The invention discloses a culture medium capable of promoting the division growth of mesenchymal stem cells, which comprises a serum-free basic culture medium and an additive added on the basis of theserum-free basic culture medium, wherein the additive comprises a hibiscus mutabilis extract, seaweed polysaccharide, astragaloside IV, human serum albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and vitamin A; the hibiscus mutabilis extract has antioxidant and cell activating activity, and can effectively promote the growth of cell metabolic by compounding seaweed polysaccharide and astragaloside IV; human serum albumin, transferrin, glutamine and vitamin A provide essential nutrient substances for the growth of stem cells; platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and other cytokines together promote the rapid growth and proliferation ofstem cells; the culture medium not only can improve the growth activity of mesenchymal stem cells, shorten the culture time, promote the expression of cell growth factors, but also can maintain the stem cell activity of the differentiation potential of mesenchymal stem cells; stem cells are not differentiated in daily culture, thereby providing convenience for scientific research.

The invention relates to a compound biological patch and a preparation method thereof. The compound biological patch comprises a macromolecular material layer and a lower matrix material layer of a mucous membrane of small intestines, wherein the macromolecular material layer comprises a macromolecular material with mesh structures, and the lower matrix material layer of the mucous membrane of the small intestines comprises collagenous fibers and growth factors. By fusing the macromolecular material with animal tissues, the compound biological patch has relatively high mechanical properties, and the animal source portion retains a natural ECM three-dimensional structure, so that the compound biological patch is low in immunogenicity and strong in anti-infection ability.

The invention discloses a method for separating purified bdellovibrio from active sludge. By virtue of key techniques of host bacteria screening and active sludge pretreatment and technical steps such as preparation of a culture medium and a culture solution and optimization of culture conditions and the like, efficient bdellovibrio is separated and purified from a homologous sludge sample by optimization of a series of key steps by virtue of gram negative bacteria which are obtained in active sludge as host bacteria for separating and purifying bdellovibrio. The invention not only puts forwards a method for successfully separating the efficient bdellovibrio from the active sludge of a municipal wastewater treatment plant for the first time, but also the culture period of the method is remarkably shortened, so that the defects that the existing conventional method is unstable in bdellovibrio separating effect, long in culture period, tedious in operation and the like.

The invention discloses a biomedical mouse tail collagen extraction method. A process combining an acid method with an enzymic method is adopted, a constant low-temperature aseptic environment is kept, a salt solution at the same concentration is used for twice salting out and centrifugation, so that purification steps are simplified. Adopted acid is a low-concentration acid solution, and citric acid is used for purification instead of acetic acid. From raw material extraction to sample generation, vacuum freeze drying is performed for many times, collagen micro powder with water content of lower than 3 percent is obtained by further treatment, and is finally sterilized and aseptically packaged, and the purity of a collagen powder finished product is over 98 percent. According to the method, the immunogenicity of the collagen is reduced, yield and biocompatibility are improved, the cost is reduced, the method is applied to a biomedical material, and a peculiar triple helix structure of the obtained collagen can be completely preserved.

The invention discloses a multistage through-hole porous tooth implantation body. The multistage through-hole porous tooth implantation body comprises a gingiva passing through segment and a screw thread segment; the gingiva passing through segment is of a smooth solid structure, and is arranged on the upper part of the screw thread segment; the multistage through-hole porous tooth implantation body also comprises a porous structure segment; the porous structure segment is arranged on the lower part of the screw thread segment; the hole diameter of transverse through holes on the porous structure segment from the lower part to the upper part is increased successively; the transverse through holes with the same hole diameter are arranged in a radiation manner along the periphery of the porous structure segment. The multistage through-hole porous tooth implantation body is capable of increasing combination strength, increasing combination speed, and is provided with the porous structure conveniently.

The invention discloses an inducing method for danio-rerio inductivity multi-potential stem cells. The induction method includes the following steps that danio-rerio tissues or danio-rerio embryos are gotten, and danio-rerio fibroblasts are obtained through in-vitro isolated culturing; the danio-rerio fibroblasts are infected through viruses, the infected danio-rerio fibroblasts are induced, and the danio-rerio inductivity multi-potential stem cells are obtained through manual selecting cloning and subculturing. Based on the same technical scheme, the invention further discloses an inductive culture medium and an iPS culture medium used for the inducing method. RT-PCR analysis indicates that iPS cells induced through the embryo fibroblasts have the potency of being differentiated into various triploblastic-source cells. By means of the method, the inductive culture medium and the iPS culture medium, the technical difficulty that the infection efficiency of slow viruses in fish cells is low is overcome, and new tool cell sources and new concepts are provided for researching a danio-rerio somatic-cell reprogramming mechanism to select cell materials.

The invention belongs to the technical field of food, and particularly relates to a probiotics powder and a preparation method thereof. The probiotics powder of the invention comprises the following components in parts by weight: 8-16 parts of Pu'er tea enzyme powder, 10-18 parts of multiple fungus powder, 6-8 parts of soybean protein isolate powder, 6-12 parts of spirulina powder, 12-20 parts ofmodified yeast glucan, 2-6 parts of black bamboo salt, 4-8 parts of honey and 32-46 parts of fructo-oligosaccharide. The probiotics powder provided by the invention is reasonable and scientific in compatibility, and the components interact with each other. The probiotics powder not only has a good taste and good stability, but also has the effects of moistening intestines, relaxing bowels, expelling toxins, lowering fat and reducing weight.

The invention relates to nucleated pearl lamella conditioning fluid in the technical field of pearl culture and application of the nucleated pearl lamella conditioning fluid. The conditioning fluid comprises the following constituents, by mass-to-volume concentration percentage, 1-5% of acid red, 0.01-5% of chondroitin, 0.1-5% of mafenide, 0.1-5% of taurine, 0.75% of sodium chloride, 0.1-5% of hyriopsis cumingii polysaccharide, and 0.05-3% of vitronectin, and allowance being water. The invention further relates to the application of the nucleated pearl lamella conditioning fluid in the pearl culture. The nucleated pearl lamella conditioning fluid can strengthen cell viability, prevent cell infection, and accelerate cell division and wound healing. When the nucleated pearl lamella conditioning fluid is applied to culture pearls, the death rate of the pearls is obviously reduced, and the rate of remaining nucleuses is obviously improved. Meanwhile, lamellas can be rapidly attached to lamella insertion shells, and the lamella insertion shells can rapidly proliferate to grow into pearl sacs. The nucleated pearl lamella conditioning fluid is simple in method, easy to achieve, wide in application range and remarkable in effect, is used for cultivating nucleated pearls by fresh water pearl mussels, and has wide application prospects.

The invention relates to a special-purpose culture medium for culturing neural stem cells and a culture method thereof. The neural stem cell special-purpose culture medium is based on a DMEM/F12 culture medium and comprises the following concentration components: 300-450[mu]g/ml of 2',3'-dideoxyadenosine-5-triphosphate, 5-15[mu]g/ml of a N2 additive, 15-30ng/ml of stem cell growth factors, 5-7mg/ml of thioglycerol, 50-70ng/ml of nerve growth factors, 0.05-0.07mmol/ml of acetylcholine, 10-25mg/ml of sodium carboxymethylcellulose, 0.005-0.01mmol/ml of retinoic acid and 0.110-0.20mmol/ml of folic acid. The culture medium formula is reasonable and effective. The undifferentiated state of neural stem cells can be maintained and the cell expansion rate is effectively improved. The differentiation performance is not influenced and the passage number is prolonged.

The invention relates to the field of medicines, and in particular relates to a cooling blood callus prescription extractive and a compounded resveratrol cooling blood callus oil preparation for treating skin burn. The callus prescription extractive comprises the following raw materials by weight: 30-50g of goldthread, 30-50g of golden cypress, 30-50g of radix scutellariae, 20-40g of rheum officinale, 20-40g of radix sophorae flavescentis, 20-40g of the root bark of shaggy-fruited dittany, 10-30g of periostracum cicada, 5-15g of cuttlebone, 50-80g of lithospermum, 20-40g of garden burnet, 10-30g of red flower and 10-20g of earthworm. The compounded resveratrol cooling blood callus oil preparation is prepared by combining the callus prescription extractive and resveratrol. The preparation has the anti-inflammatory and debridement effects, can not only cover the wound surface, reduce infection, but also can actively participate in the burn wound healing, thus greatly improving the treating effect of wound surfaces of burn, trauma and the like.

The invention relates to a biochemical water purifying agent, in particular to a composite enzyme biochemical water purifying agent and a preparation method thereof, and belongs to the field of polluted water. The composite enzyme biochemical water purifying agent is prepared from the following components in percentage by weight: 20 to 30% of a multi-enzyme compound, 1 to 6% of a diatomite extract, 3 to 5% of chitin, 2 to 10% of a complexing agent, 50 to 70% of deionized water, 2 to 10% of a carbon source, 1 to 5% of dodecyl dimethyl benzyl ammonium chloride, and 0.05 to 0.1% of polyacrylamide, wherein the sum of weight percent of all components is 100%. The composite enzyme biochemical water purifying agent has the advantages that the water purifying agent can be used for effectively catalyzing and degrading according to the features of different pollutants; the formula of the product is poisonless and harmless, and the environment-friendly effect is realized; the production technology is concise, scientific and reasonable, and the environment-friendly effect is realized; the water purifying agent is suitable for the fields of eutrophic water body, domestic sewage, industrial wastewater, cleaning and deodorizing of communities, cleaning and deodorizing of garbage, composting of garbage, treatment of garbage percolate, treatment of wastewater in culture industry, soil improvement, daily cleaning of road vehicles, and the like.

The invention relates to a method of simulating bone marrow microenvironment to culture hematopoietic stem cells. The method includes following steps: acquiring hematopoietic stem cells, and inoculating the hematopoietic stem cells onto bio-derived bone containing osteoblasts for in-vitro amplification culture. The bio-derived bone containing the osteoblasts is adopted as a three-dimensional culture scaffold, and the hematopoietic stem cells and the osteoblasts are co-cultured in the bio-derived bone, so that contact area between the hematopoietic stem cells and a culture medium as well as osteoblast juxtacrine is increased, and growing and proliferating of the hematopoietic stem cells are facilitated; the osteoblasts can secrete factors conducive to the hematopoietic stem cells to maintaining characteristics, and the hematopoietic stem cells after amplification can still have potential of multidirectional differentiation, so that the problems of low cell activity and low proliferation rate when a two-dimensional culture system is adopted to culture the hematopoietic stem cells and the problem that the hematopoietic stem cells are prone to losing the stem cell characteristics in the prior art are solved.

The invention provides a vaginal expansion suppository containing probiotics and a traditional Chinese medicine composition, and a preparation method thereof. The vaginal expansion suppository comprises an expansion carrier and a drug-containing matrix coating the surface of the expansion carrier; the drug-containing matrix comprises probiotics, the traditional Chinese medicine composition and a matrix; the probiotics comprise lactic acid bacteria and bifidobacteria; the traditional Chinese medicine composition comprise cortex phellodendri, radix sophorae flavescentis, fructus cnidii, folium artemisiae argyi, radix scutellariae, radix astragali, rheum officinale and ginseng. According to the vaginal expansion suppository disclosed by the invention, through an administration form of combining medicinal components with the expansion carrier, full contact between the suppository and a lesion part and absorption of the lesion part on medicines are promoted, so that the vaginal expansion suppository is high in stability and good in curative effect; and the synergistic compounding of the effective medicinal components probiotics and the traditional Chinese medicine composition promotes the growth and proliferation of effective microbial floras in the vagina, and inhibits and eliminates pathogenic bacteria in a broad-spectrum manner, so that the vagina microbial community environmentis thoroughly improved, and the micro-ecological health in the vagina is maintained.