Method of promoting haematococcus pluvialis growing multiplication and regulating astaxanthin synthesis and accumulation in photobiological reactor

A technology of photobioreactor and Haematococcus pluvialis, applied in biochemical equipment and methods, microorganisms, fermentation, etc., can solve the problems of light transmittance decline, biological pollution, low population density, etc., and achieve the regulation of synthesis and accumulation , high utilization rate of light energy, and the effect of promoting growth and proliferation

Inactive Publication Date: 2003-01-22
SUN YAT SEN UNIV
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main factors hindering the large-scale production of Haematococcus pluvialis are the high cost caused by the low population density of Haematococcus pluvialis culture process and the low yield per unit area.
Haematococcus pluvialis is cultivated in small-scale bioreactors or airlift bioreactors composed of pipe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example one:

[0020] Established an all-weather closed large-scale operation system photobioreactor, the main reactor (tank) is 500×150×65cm in length, width and depth, the bottom and surroundings are made of 2cm thick colorless and transparent tempered glass, and the cover part adopts a thickness of 0.2mm The transparent film is sealed with tape. The main reactor is installed around and up and down with 20 100W solar lamps and 20 40W energy-saving spotlights. The speed of the speed-adjustable impeller stirrer in the reactor is 30r / m; The speed of the water scroll wheel is 50r / m, and the salt and fresh water nutrient solution circulates at a flow rate of 0.04m / s; the salt and fresh water nutrient solution blending and temperature control system is equipped with 3 liquid storage tanks with a capacity of 4000L, and the agitator speed is 80r / m. The culture conditions for the growth and proliferation of algae cells and the increase in population density regulation stage are as...

Example Embodiment

[0021] Embodiment two:

[0022] Establish an all-weather closed large-scale operation system photobioreactor, the main reactor (tank) is 220×110×55cm in length, width and depth, 1.5cm thick colorless and transparent special glass is used at the bottom and around, and the cover part uses a thickness of 0.1 mm transparent film and sealed with tape. The main reactor is installed around and up and down with 20 adjustable brightness power of 40W fluorescent lamps; the speed of the speed-adjustable impeller agitator in the reactor is 20r / m; the rotating speed of the water wheel is 30r / m, the brackish water nutrient solution circulates at a flow rate of 0.02m / s; the brackish water nutrient solution preparation and temperature control system is equipped with two storage tanks with a capacity of 1200L, and the agitator speed is 60r / m. The culture conditions for the growth and proliferation of algae cells and increase the population density are as follows: the composition of brackish water...

Example Embodiment

[0023] Embodiment three:

[0024] Establish an all-weather closed large-scale operation system photobioreactor, the main reactor (tank) is 200×100×65cm in length, width and depth, and 1cm thick colorless and transparent tempered glass is used at the bottom and surroundings, and the cover part is 0.2mm thick. The transparent film is sealed with adhesive, the main reactor is installed around and up and down. The power of adjustable brightness is 7 40W fluorescent lamps and 5 100W solar lamps; the speed of the speed-adjustable impeller in the reactor is 10r / m; the water wheel The speed is 10r / m, and the salt and fresh water nutrient solution circulates at a flow rate of 0.03m / s; the salt and fresh water nutrient solution blending and temperature control system is equipped with two liquid storage tanks with a capacity of 800L, and the agitator speed is 20r / m. The culture conditions for the growth and proliferation of algae cells and increase the population density are as follows: the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to view more

Abstract

The present invention relates to the process of promoting haemtococcus pluvialis growth, raising its population density and controlling the synthesis and accumulation of astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, bacteria-free ventilating and oxygen unit, salty and fresh water nutritious liquid compounding and temperature controlling unit, and cleaning and sterilizing unit; altering environment conditions to induce haemtococcus pulvialis to multiply, raise population density and regulating synthesis, accumulation and collecting haemtococcus pluvialis with rich astaxanthin.

Description

technical field [0001] The invention relates to a method for cultivating and promoting the growth and proliferation of Haematococcus pluvialis, increasing the population density, and regulating the synthesis and accumulation of intracellular secondary metabolite astaxanthin in an adjustable all-weather closed large-scale operation system photobioreactor. Background technique [0002] Haematococcus pluvialis is a single-celled green alga with oval or oval cells ranging in size from about 5 microns to 20-30 microns, sometimes even larger; it has two flagella of equal length , the swing of the flagella pushes the cells to swim freely; there is a gap between the cell wall and the protoplast, which is filled with gelatinous substances, and there is a cytoplasmic filament connection between the cell wall and the protoplast, and the vegetative reproduction is cell division, forming 2, 4, 8, 16 or even more Multiple daughter cells, asexual reprodu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/02C12P23/00
Inventor 刘昕王江海袁建平黄晓霓
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap