124results about How to "Optimize culture conditions" patented technology

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time: quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

The present invention relates to the process of promoting haemtococcus pluvialis growth, raising its population density and controlling the synthesis and accumulation of astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, bacteria-free ventilating and oxygen unit, salty and fresh water nutritious liquid compounding and temperature controlling unit, and cleaning and sterilizing unit; altering environment conditions to induce haemtococcus pulvialis to multiply, raise population density and regulating synthesis, accumulation and collecting haemtococcus pluvialis with rich astaxanthin.

The invention belongs to the technical field of biology engineering and particularly discloses an actinomycete strain KN37 and an application thereof. Through microscopic observation and 16S rDNA sequence analysis, the condition that the separated actinomycete strain KN37 belongs to Streptomyces is identified, and the preservation number of the actinomycete strain KN37 is CGMCC No.13160. A fermentation broth of the strain KN37 has a better inhibition effect on multiple phytopathogens such as Botrytis cinerea, Alternaria solani, Fusarium oxysporum, Fulvia fulva and the like and can be used for preparing microbial agents for controlling crop diseases.

The invention relates to a preparation method and application of a marine fungi aspergillus terreus butyrolactone compound butyrolactone-I. The invention discloses application of the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I as shown in a formula (I) to preparation of medicaments for resisting to peripheral and neurogenic inflammation and resisting to neurodegenerative diseases. The formula is shown in the description. The invention finds that besides a DPPH free radical, butyrolactone-I can also well remove an ABTS free radical and an OH free radical; the marinefungi aspergillus terreus butyrolactone compound butyrolactone-I has better oxidation resistance and inflammation resistance and has better neuroprotection activity; therefore, the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I has a wide application prospect in the aspect of preparing the medicaments for resisting to peripheral and neurogenic inflammation and resisting toneurodegenerative diseases. Meanwhile, by the preparation method of the butyrolactone-I, which is provided by the invention, the butyrolactone-I can be successfully prepared; the method is simple andeasy to realize large-scale production; culture conditions can also be optimized by addition of an inducer, so that yield of the butyrolactone-I is greatly improved.

The invention discloses a preparation method and applications of freeze-dried powder of acetic acid bacteria. The preparation method comprises following steps of: 1) inoculating the acetic acid bacteria into a liquid culture medium and culturing at 28-30 DEG C for 10-30 h to obtain a primary strain; 2) inoculating the primary strain into a fermentation culture medium, culturing at 28-33 DEG C for 10-30 h, and adding an aqueous sodium hydroxide solution having a concentration of 5-25 wt% during the fermentation process to obtain a fermentation liquid mixture; 3) filtering the fermentation liquid mixture or centrifuging and separating to obtain bacterial sludge; 4) uniformly mixing the bacterial sludge and a bacterial sludge protective agent to obtain a bacterial suspension; and 5) freeze-drying the bacterial suspension to obtain the freeze-dried powder of the acetic acid bacteria. After the freeze-dried powder of the acetic acid bacteria is mixed with various additives, alcoholism-relieving tablets and alcoholism-relieving beverages, which have good alcoholism-relieving effects, can be obtained through different processes. The preparation method is simple and the freeze-dried powder of the acetic acid bacteria is high in bacterium content and long in storage time.

The invention relates to an asparagus extract bifidobacterium fermentation activity beverage and a preparation method thereof. An asparagus extract, brown sugar and stachyose serve as a culture medium; the following strains as shown in the specification are subjected to anaerobic fermentation to culture a bifidobacterium; after the anaerobic fermentation, a fermented probiotic liquid is subjected to sterile filling; and the bifidobacterium, lactobacillus plantarum, lactobacillus acidophilus and saccharomyces cerevisiae are freeze-dried bacteria bought from a culture collection center. The beverage supplements a specific bifidus factor and an exogenous bifidus factor in a plant in order to culture high-density bifidobacterium longum, increase the unit bacterial count of other lactic acid bacteria, conduct enzymolysis of small protein converting peptide in the culture medium, increase the content of amino acid essential for a human body, and improve the activity of asparagus juice, and a health care function of the whole fermentation liquid. The technology has the advantages that a strict anaerobic condition of the bifidobacterium is overcome, an expensive bifidobacterium fermentation device is removed, and the health care function of metabolic products of an active substance and a microorganism containing asparagus, as well as the fermentation liquid of the viable bacteria is enhanced.

The invention discloses a method for preparing a heat-resisting attenuated virus live vaccine for goatpox by using a BHK21-C13 passage cell. Based on the existing attenuated virus AV41 for goatpox, which has excellent immunogenicity, the method comprises the following steps: culturing 25-28th generations of virus solution with a heterologous BHK21 cell, and then adding an appropriate heat-resisting freeze-drying protective additive to obtain the heat-resisting attenuated virus live vaccine for goatpox. The passage cell vaccine used by the method is superior to the primary cell vaccine in the aspects of virus yield, homogeneity, purity and the like, not only guarantees the effective level of the vaccine and has a better heat-resisting protection effect, but also can ensure the safety of a purebred pregnant goat. As the existing BHK21 cell adopts a mature suspension culture process, the exploration of various parameters of adherent culture lays a solid foundation for the next promotion of suspension process.

The invention relates to a liquid strain culturing method and culturing method of boletus aereus. The method comprises the steps that liquid strains of boletus aereus are inoculated to a fermentationtank, and after six stages of culturing of strain mycelium breaking, mycelium restoring, culturing adapting germinating, mycelium growing and mycelium exponential growing period are conducted, fermentation is completed. According to the method, different culturing conditions are made aiming at the growing characteristics of germ in different stages, and the boletus aereus is adapted to growth in the fermentation tank to obtain higher growing rate; culturing conditions of the boletus aereus liquid strains in the fermentation tank are studied, mechanical inoculation feasibility is provided, andthe method has higher production efficiency compared with an existing triangular flask culturing method and has a lower pollution rate compared with a solid grain culturing method.

The invention relates to a CYP101 enzyme recombinant vector, a construction method thereof and a CYP101 enzyme high-efficiency expression and purification method, and belongs to the field of biotechnology. A PET28a-CYP101 high-efficiency expression vector is constructed and transformed into BL21 plysS strain, and high-purity enzyme with purity higher than 95% is obtained by a simple two-step purification method including affinity chromatography and ion exchange chromatography after inducible expression. Compared with the prior art, CYP101 is simply and efficiently produced, the cost is low, purification is facilitated, and 23mg of target protein with the purity higher than 95% can be obtained by per liter of culture media. The CYP101 enzyme high-efficiency expression and purification method can be used for expressing and purifying CYP101 enzyme, the production efficiency of a laboratory is greatly improved, and experimental cost is greatly reduced. The CYP101 enzyme high-efficiency expression and purification method has an excellent application prospect for large-scale CYP101 enzyme production and purification.

The invention relates to the fields of gene engineering and biosynthesis, and concretely relates to a construction body for efficiently synthesizing ethylene, and a construction method and an application thereof. The construction body comprises an active promoter and an ethylene synthetase gene (efe) controlled by the promoter. Carbon dioxide can be immobilized by above engineering bacterium through using solar energy in order to synthesize ethylene. An ethylene synthesis approach is constructed in cyanobacterial cells in order to realize immobilization of carbon dioxide in photosynthetic microbal cyanobacterial cells through using solar energy and synthesis of ethylene, wherein energy for ethylene synthesis is from the solar energy, and a carbon source is carbon dioxide. The method can be used to prepare bioethylene without restriction of petroleum shortage, and the bioethylene does not increase carbon emission.

The invention relates to a method used for co-culturing of ethanol high yield Saccharomyces cerevisiae YF1914 and an ethyl acetate high yield Wickerhamomyces anomalus YF1503 bacterial strain, and applications thereof. The Saccharomyces cerevisiae YF1914 is preserved in China General Microbiological Culture Collection Center on Oct. 20th 2017, and the preservation number is CGMCC No.14827; the sequence similarity of 26S rDNA D1/D2 sequence of the Saccharomyces cerevisiae YF1914 and 26S rDNA D1/D2 sequence of a plurality of other Saccharomyces cerevisiae bacterial strains is relatively high; ina sorghum enzymatic hydrolysate culture medium, co-culturing of the two yeast bacterial strains is benefical for increasing of ethyl acetate content. The double bacterial strain co-culturing method can be applied in brewage industry such as baijiu, yellow rice wine, and soy sauce with requirements on ethyl acetate.

The asymmetrical resolution method for (R)-mandelic acid comprises: screening the (Brevibacterium flavum) AS 1.818 for culture and full cell preparation; with racemic acid as substrate, catalytic converting to obtain the product with optical purity up to 90%e.e. This invention improves product optical purity and has important meaning for enzyme development and resolution.

The invention discloses a piriformospora indica fermentation method. The method comprises the following steps: by taking a PDB culture medium as a culture solution, regulating the pH of the culture solution to be 5.0-5.1 by using a HCl solution with the concentration of 1mol/L, and performing culture fermentation on piriformospora indica at the temperature of 27.5-28.5 DEG C for 14-15 days, so as to obtain the fermentation liquor. The invention also discloses a method for performing response surface optimization in the piriformospora indica fermentation process. The method comprises the following steps: setting the three variables including the pH, the concentration of the PDB culture medium and the culture time; optimizing according to the Box-Behnken design principle by utilizing design expert software. According to the method, the yield of secondary metabolites can be increased, and the consumption of nutritional ingredients and culture media is reduced.

The asymmetrical reduction bio-method for (R)-mandelic acid comprises: screening the Saccharomyces cerevisiae AS 2.150 for culture and full cell preparation; with benzoylformic aicd as substrate, adding assist substrate, catalytic converting to obtain the product with optical purity up to 90%e.e. This invention improves product optical purity and yield, and has important meaning for enzyme development and resolution.

The invention relates to aeribacillus pallidus Jh-7 antagonizing xanthomonas campestris pv. oryzae. The invention aims at providing the aeribacillus pallidus Jh-7 antagonizing the xanthomonas campestris pv. oryzae firstly. The aeribacillus pallidus contains an ingredient for inhibiting the xanthomonas campestris pv. oryzae, and a good approach is provided for biological control of rice diseases caused by the xanthomonas campestris pv. oryzae. The invention aims at providing a metabolism crude extract of the aeribacillus pallidus Jh-7 secondly. The invention aims at providing an application ofthe metabolism crude extract of the aeribacillus pallidus Jh-7 thirdly. The invention aims at providing a biological pesticide containing the aeribacillus pallidus Jh-7 fourthly.

The invention relates to the technical field of fermentation industry, in particular to a preparation method of selenium-rich yeast. A soil sample from Bama County, Guangxi is selected, the content ofselenium in Bama soil and grains is 10 times or more higher than the national average, a selenium-rich yeast strain is obtained through screening and breeding of yeast strains in the soil sample, theselenium ion concentration and the culture condition are optimized, and the selenium content of the yeast is increased. The selenium-rich yeast obtained through separation and screening of the selenium-containing soil is subjected to domestication with sodium selenite with gradient concentrations, yeast colonies with high selenium resistance are screened, then, ultraviolet-induced mutation is performed, the selenium enrichment capability of the yeast is enhanced, and the yeast with high biomass and selenium enrichment capability is obtained.

The invention belongs to the technical field of microorganisms, and relates to marine-derived Aspergillus terreus M7 with an antibacterial effect, and separation and application of a secondary metabolite of the Aspergillus terreus M7. The strain is classified and named as Aspergillus terreus M7, and is preserved in China Center for Type Culture Collection (CCTCC) on June 7, 2021, with the preservation number of CCTCC NO: M 2021679. The strain is fermented by a soybean solid culture medium, and is subjected to methanol extraction, silica gel G chromatography, Sephadex LH-20 gel column chromatography, silica gel H chromatography and preparative liquid phase separation and purification to obtain a compound terramide A. The terramide A has obvious antibacterial activity on acinetobacter baumannii ATCC 19606, and has the potential of being developed into an antibacterial agent.

The invention relates to a one-step seedling and efficient in-vitro propagation method for Begoniaceae gynura bicolor, which is an endangered plant with homology of medicine and food. The method provided by the invention comprises the following steps of: preparing a culture medium, and adjusting the culture medium through preparing, subpackaging and sterilizing; carrying out sterile inoculating directly on gynura bicolor aseptic seedling leaves which are taken as explants; culturing the inoculated culture medium in a culture chamber for 70-80 days under appropriate conditions, wherein the number of a single explant induced seedlings is 20 above; and decapping, transplanting regenerating plants to appropriate conditions, and culturing without hardening-seedling, wherein the commodity seedling survival rate achieves 100%. According to the method provided by the invention, the gynura bicolor tissue culture seedling regeneration period is short; the original seed property is kept effectively; the vegetative propagation coefficient is improved obviously; the operation is simple and convenient; the culture program is simple; the seedling cost is lowered obviously; and the method can act as an effective technology of industrialized production of high-quality seedlings of Begoniaceae gynura bicolor.

The invention discloses a camellia changii variety breeding method which comprises the following steps of selecting budding flowers by taking fragrant flowers with rich flower colors and flower types as breeding aims in a camellia changii male parent blooming season, poking petals, quickly removing all stamens, smearing pollens to be pollinated on stigmas, folding an adhesive plaster in two to completely cover the stigmas for controlled pollination to realize the controlled pollination of theaceae plants, and performing seed breeding of hybrids under an indoor control condition. The variety of camellia changii is small, the number of female parents is small, and the breeding potential is low. By a tissue culture technology disclosed by the invention, the problems can be well solved; rare and precious resources, namely the camellia changii, can be protectively used; the problem that the camellia changii seedlings are scarce can be alleviated.