Liquid strain culturing method and culturing method of boletus aereus

A technology of black boletus and liquid strains, which is applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of bacterial culture pollution, long cultivation period, inability to carry out mechanical inoculation, etc., to improve growth rate and efficiency. Effect

Active Publication Date: 2018-12-07
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Black boletus Phlebopus portentosus (Berk&Broome) Boedijn, also known as dark reticulate boletus, belongs to Boletinellaceae (Boletinellaceae), delicious taste, rich nutrition, its water extract has anticancer and antiviral properties Function, and rich in mineral elements and a variety of essential amino acids for the human body, it is deeply loved by consumers because of its edible and medicinal value. The number of natural picking is very small, which cannot meet the market demand. The existing technology has been completed Black liver fungus factory cultivation technology model, and factory cultivation is an inevitable trend
However, the cultivation period of the solid strain of Boletus nigra is long, and it is difficult to control a large amount of pollution during the cultivation period due to the invisible pollution of the solid strain.
At present, the liquid strains of boletus are mainly cultivated in Erlenmeyer flask shake flasks. The efficiency of obtaining liquid strains of bacteria in Erlenmeyer flask shake flasks is low, and mechanical inoculation cannot be performed. At present, manual inoculation is relied on, which is not suitable for industrial scale cultivation.
[0003] Fermentation tank culture technology has the advantages of short production cycle, consistent bacterial age, low strain cost, etc., and can be mechanically inoculated. However, there are few reports on the fermentation tank culture technology of black boletus

Method used

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  • Liquid strain culturing method and culturing method of boletus aereus

Examples

Experimental program
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Effect test

Embodiment 1

[0057] 1. Mother species activation

[0058] Mother species activation medium formula (referred to as M1):

[0059]

[0060] M1 preparation: Accurately weigh the above-mentioned medicines, wash and peel the potatoes, cut them into thin slices, weigh the potatoes according to the required amount, take a clean iron basin and add 200mL more water to the required nutrient solution, put the weighed potatoes, heat to boil until When the potatoes become soft, filter the potato boiled juice with 4 layers of gauze, dissolve the drug in the filtered potato boiled juice, divide it into test tubes at a fixed volume, sterilize at 115°C for 15 minutes, put it on a slope, and cool it for later use.

[0061] parent species activation

[0062] Inoculate the preserved mother species in the above-mentioned (2) test tube slant medium, culture at 28°C for 30 days, the hyphae will cover the slant, and the activation of the mother species will be completed.

[0063] Second, the first-level liqu...

Embodiment 2-3

[0089] Bacterial strain activation, one-level liquid seed cultivation, liquid strain fermentation culture method and fruiting verification are identical with embodiment 1, difference is: the fermentor volume 750L / tank of embodiment 2, the fermenter volume 1000L / jar of embodiment 3 Can. After the culture of the strain in the fermenter is completed, the machine is used for inoculation.

Embodiment 4

[0091] Strain activation, first-level liquid seed cultivation, liquid strain fermentation culture method and fruiting verification are the same as in Example 1, except that the liquid strain fermentation conditions are as follows:

[0092] (1) The hyphae of the strain (ball) are broken to increase the germination point: adjust the culture temperature to 16°C, the ventilation pressure to 0.025Mpa, stop the ventilation after 0.75h of ventilation, adjust the motor speed to 5000ramp / min, and cultivate for 2.5h.

[0093] (2) Mycelia recovery: After the mycelium is broken, adjust the appropriate medium temperature (23°C), medium speed (550ramp / min) and ventilation pressure of 0.04Mpa, and cultivate for 10h to allow the mycelia to recover.

[0094] (3) Cultivate to adapt to germination: adjust the temperature to 29.5°C, the ventilation pressure to 0.06Mpa, the rotation speed to 475 / min, and cultivate for 23 hours to allow the hyphae to germinate;

[0095] (4) Mycelia growth: adjust t...

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PUM

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Abstract

The invention relates to a liquid strain culturing method and culturing method of boletus aereus. The method comprises the steps that liquid strains of boletus aereus are inoculated to a fermentationtank, and after six stages of culturing of strain mycelium breaking, mycelium restoring, culturing adapting germinating, mycelium growing and mycelium exponential growing period are conducted, fermentation is completed. According to the method, different culturing conditions are made aiming at the growing characteristics of germ in different stages, and the boletus aereus is adapted to growth in the fermentation tank to obtain higher growing rate; culturing conditions of the boletus aereus liquid strains in the fermentation tank are studied, mechanical inoculation feasibility is provided, andthe method has higher production efficiency compared with an existing triangular flask culturing method and has a lower pollution rate compared with a solid grain culturing method.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a liquid strain cultivation method and cultivation method of black boletus. Background technique [0002] Black boletus Phlebopus portentosus (Berk&Broome) Boedijn, also known as dark reticulate boletus, belongs to Boletinellaceae (Boletinellaceae), delicious taste, rich nutrition, its water extract has anticancer and antiviral properties Function, and rich in mineral elements and a variety of essential amino acids for the human body, it is deeply loved by consumers because of its edible and medicinal value. The number of natural picking is very small, which cannot meet the market demand. The existing technology has been completed Black liver fungus factory cultivation technology model, and factory cultivation is an inevitable trend. However, the cultivation period of the solid strain of Boletus nigra is long, and it is difficult to control the large amount of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 魏学林张倩李春会高丽霞罗顺珍纪光燕纪光玉玉应香
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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