The invention relates to a black truffle kidney tonifying formula which comprises the following components in parts by weight: 20-30 parts of black truffle, 10-20 parts of cordyceps militaris, 1-5 parts of cinnamon, 5-10 parts of gordon euryale seeds, 1-5 parts of tricholoma matsutake, 5-10 parts of oysters, 10-15 parts of rhizoma polygonati, 1-5 parts of sarcodon aspratus, 1-5 parts of schizophyllumcommuneh, 1-5 parts of boletus aereus and 5-10 parts of poria cocos. According to the formula, the black truffle is used as the monarch to warm and tonify kidney yang, nourish liver and kidney, tonify spleen and qi and tonify yin and yang, the tricholoma matsutake, the sarcodon aspratus, the cordyceps militaris, the schizophyllumcommuneh and the boletus aereus are used as the minister to assist the black truffle to relax muscles and stimulate blood circulation, strengthen the body, balance yin and yang and reduce internal heat and soothe the nerves, and the oyster, the rhizoma polygonati, the gordon euryale seed, the cinnamon, the poria cocos and the like are used as the assistant to seek yang from yin, harmonize five internal organs, tonify kidney and warm yang. The traditional Chinese medicine is suitable for people with kidney-yang deficiency, easy fatigue, sexual function reduction and need of supplementing physical strength and energy.