Method for preparing water-soluble boletus aereus melanin

A technology of black boletus and melanin, applied in chemical instruments and methods, functions of food ingredients, azo dyes, etc., can solve problems such as limited application

Inactive Publication Date: 2019-02-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As one of the active components of black boletus, melanin has the functions of anti-venom, inhibiting the replication of HIV, and treating Parkinson's disease. However, melanin is usually only soluble in alkaline solution, which limits its application in food deep processing. Therefore, the research on black boletus melanin has very important practical value and theoretical significance for the high value-added comprehensive utilization and deep processing development of black boletus

Method used

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  • Method for preparing water-soluble boletus aereus melanin
  • Method for preparing water-soluble boletus aereus melanin

Examples

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Effect test

Embodiment 1

[0013] The black boletus melanin was dissolved in 0.10M NaOH in the ratio of 1g melanin:50-100mL NaOH solution, then quickly adjusted to pH 8.0 with 0.10M HCL, centrifuged at 10000 r / min, and the supernatant was filtered through 0.22μm Membrane, the filtrate is collected, ultrapure water is dialyzed in running water for 48 hours to remove the salt, and then freeze-dried to obtain the water-soluble Boletus edulis melanin.

Embodiment 2

[0015] The melanin of Boletus edulis was dissolved in 0.14 M NaOH in the ratio of 1g melanin:50-100mL NaOH solution, and then quickly adjusted to pH 8.0 with 0.14 M HCL, centrifuged at 10000 r / min, and the supernatant was filtered through 0.22μm Membrane, collecting the filtrate, dialysis with ultrapure water for 48 hours to remove salt, and drying at room temperature to obtain water-soluble boletus black melanin.

Embodiment 3

[0017] The black boletus melanin was dissolved in 0.20M NaOH in the ratio of 1g melanin:50-100mL NaOH solution, then quickly adjusted to pH 8.0 with 0.20M HCL, centrifuged at 10000 r / min, and the supernatant was filtered through 0.22μm Membrane, collecting the filtrate, dialysis with ultrapure water for 48 hours to remove salt, and vacuum drying to obtain water-soluble Boletus edulis melanin.

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Abstract

The present invention provides a simple method for preparing water-soluble boletus aereus melanin. The method is as follows: a certain amount of boletus aereus melanin is dissolved with 0.1-0.2 M NaOH; pH is rapidly adjusted to 8.0 by 0.1-0.2 M HCL; centrifugation is conducted at 10,000 r/min for 3 min; a supernatant passes through a 0.22 [mu]m filter membrane; a filtrate is collected; and the collected filtrate is freeze-dried to obtain the water-soluble melanin. The obtained boletus aereus melanin has very good water solubility, solves a problem that the melanin extracted by the conventionalmethod cannot be melted into water, and has very important practical value for the deep processing development of the boletus aereus melanin.

Description

Technical field [0001] The invention belongs to the technical field of melanin solubility modification methods, and specifically relates to a method for modifying Boletus edulis melanin into water-soluble melanin. Background technique [0002] Black boletus (Boletus aereus, BA) is a kind of boletus and one of the most fragrant boletus. Boletus edulis is delicious, rich in nutritional value, high in protein, rich in essential amino acids and rich in mineral elements necessary for the human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. It is of high nutritional value and medicinal use. It has the effects of relieving cough, preventing cancer, tonifying qi, and has good curative effects on high cholesterol, high blood pressure and hyperlipidemia. It is deeply loved by consumers. As one of the active ingredients of Boletus edulis, melanin has anti-snake venom, inhibits HIV replication, and treats Parkinson’s disease, but usually melanin is only soluble in al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/10C09B61/00
CPCA23V2002/00A23L33/10C09B61/00C09B68/20A23V2200/30A23V2250/208
Inventor 傅俊生侯若琳刘鑫郑明峰吴小平
Owner FUJIAN AGRI & FORESTRY UNIV
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