85 results about "Snake venom" patented technology

The method is mainly applied to the technical field of deep learning. The invention discloses an intelligent diagnosis method, device and equipment for snake venom poisoning and a medium, and the method comprises the steps: obtaining multi-modal data which comprises a wound image, a symptom text and a blood parameter; performing feature extraction processing on the wound image, the symptom text and the blood parameters to obtain image features corresponding to the wound image, semantic features corresponding to the symptom text and high-dimensional features corresponding to the blood parameters; performing alignment and fusion processing on the image features, the semantic features and the high-dimensional features through a cross-modal attention mechanism to generate fusion features; after the fusion features are input into a preset multi-task classification model, classification is carried out in a snake species label space and a toxicity category space according to the fusion features, a poisoning grade score is determined, and a diagnosis report is generated based on the poisoning grade score. According to the invention, high-precision, rapid and intelligent diagnosis of snake venom poisoning can be realized.

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to the technical field of detection kits, in particular to a blood coagulation reaction linked lupus anticoagulant fluorescence detection kit and application thereof, the kit contains a screening-confirmation integrated reagent, a calibration product, a quality control product and an anticoagulation interference inhibitor; the integrated reagent is divided into a reagent A and a reagent B, the reagent A contains a buffer solution, recombinant snake venom thrombin, composite phospholipid, a fluorescent substrate and other components, and the reagent B contains prothrombin, calcium ions, high-concentration composite phospholipid and other components; the composite phospholipid system is formed according to a specific proportion and modifies beta2-glycoprotein I, the fluorescence enhancement-stabilization system is formed by matching a Schiff base compound with a stabilizer, the calibrator is a series of lupus anticoagulant standard substances, and the quality control substance comprises simulants with different concentrations and negative control. The anti-interference capability is high, and the influence of anti-coagulant drugs and enzyme source fluctuation is reduced; the signal amplification effect is obvious, the detection sensitivity is high and the reagent stability is good; the operation is simple and convenient, screening and confirmation are integrally completed, and a conventional fluorescence detector is adapted.

The present application relates to the beer brewing technical field, especially a kind of venomous beer brewing process, including the following steps: raw material preparation and pretreatment;Saccharification;Filtering;Boiling;Rotary sedimentation;Fermentation;After mature wine, under low pressure 8.986kPa pressure, reduce the boiling point of water, ensure that the boiling point is lower than 25 degrees Celsius, concentrate wine, concentrate to 15 liters or less per 100 liters, carry out oak barrel barrel;Oak barrel barrel.The product is soft in taste, no spicy residue, alcohol burning sensation controllable;Flavor levels are rich, with fruit, flower, nut and other complex aroma;Process parameters are accurate, without low-temperature freezing equipment, small and medium-sized enterprises can replicate and the quality of stable venomous beer brewing process.

The application belongs to the technical field of biotechnology, and particularly relates to a kind of snake venom polypeptide and application thereof.The snake venom polypeptide is polypeptide DACU-1, polypeptide DACU-2 or polypeptide DACU-3.The application provides a kind of new snake venom polypeptide, including three polypeptides, and the three polypeptides all have anti-platelet aggregation activity, and have the activity of inhibiting platelet aggregation under the stimulation of ADP, arachidonic acid, collagen, thrombin and other various activators, and have the potential to be developed into anti-platelet drugs, functional foods and health products.

The invention relates to the technical field of in-vitro diagnosis, in particular to a lupus anticoagulant detection kit based on recombinant snake venom enzyme and application of the lupus anticoagulant detection kit. The probe comprises a recombinant snake venom thrombin-like enzyme, a phospholipid-quantum dot conjugate, calcium ions, sodium citrate, trehalose, polyethylene glycol, L-histidine, EDTA-2Na, a Tris-HCl buffer solution, a nanogold-carbon nanotube composite probe, a graphene oxide-chitosan composite material, a fluorescence enhancer and a stabilizer. The kit disclosed by the invention is high in detection sensitivity, strong in matrix interference resistance, excellent in recombinant snake venom enzyme stability and good in batch-to-batch consistency, the detection specificity can be accurately verified, and the problem that the performance of a traditional kit is poor is solved.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition and application thereof. The composition is prepared from a white birch bark extract, snake venom peptide and Ectoine. The white birch bark extract promotes abnormal protein removal by activating an intracellular protein ubiquitination system, and improves the skin metabolism capability; the snake venom peptide slows down expression muscle contraction through a nerve regulation mechanism, and dynamic wrinkles are effectively faded; ectoin enhances cell membrane stability, protein protection and skin stress adaptive capacity, and under the synergistic effect of Ectoin, Ectoin and skin stress adaptive capacity, the comprehensive anti-aging effects of resisting wrinkles, tightening, relieving, brightening skin color and the like can be remarkably improved.

The invention discloses a whitening and anti-aging mask formula based on dogwood. The whitening and anti-aging mask formula based on dogwood is prepared from the following raw materials in parts by mass: 5 to 15 parts of dogwood extract, 2 to 8 parts of vitamin C powder, 1 to 3 parts of polyphenol compound, 1 to 5 parts of glycerinum, 2 to 4 parts of hyaluronic acid, 3 to 9 parts of plant essence, 2 to 6 parts of vegetable oil, 4 to 12 parts of seaweed extract and 1 to 3 parts of polyether-201. 1-3 parts of nerve growth factors, 2-6 parts of DNA (deoxyribonucleic acid) repair enzyme, 0.1-0.3 part of snake venom essence, 1-3 parts of plant stem cells and 20-30 parts of deionized water, components in the snake venom essence can stimulate synthesis of collagen and increase skin elasticity, so that wrinkles and fine wrinkles are reduced, the skin looks younger and tighter, certain antioxidant capacity is achieved, and the skin care effect is good. Free radicals can be neutralized, the damage of environmental pollution and ultraviolet radiation to the skin is reduced, and the aging process of the skin is delayed.

1. Name of the product in this design: Snake Venom Detector (VENOMSCAN). 2. Purpose of this design: This design is used for snake venom detection. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition containing DNA sodium and application of the anti-aging composition. The composition is prepared from DNA sodium, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E and sodium hyaluronate. According to the emulsion disclosed by the invention, various active ingredients such as the DNA sodium, the snake venom tripeptide, the elastin peptide, the low-molecular collagen peptide, the nicotinamide, the alpha-arbutin and the purple blue bletilla striata bulb fermentation liquor are organically combined, so that multiple anti-aging and whitening effects of resisting oxidation, inhibiting melanin synthesis, promoting collagen and elastin generation, improving uneven skin color and the like can be synergistically realized. Purple blue bletilla striata bulbs are subjected to lactobacillus-candida compound fermentation, the product does not contain coarse particles after refined filtration treatment, is clarified, is stable for a long time after being added into an emulsion system, and is not easy to separate out and precipitate or cause system layering. The emulsion is light in structure, not sticky and greasy, endows skin with soft and moist touch feeling, and is suitable for various skin types including sensitive skin, and active ingredients are easy to permeate and absorb.

The application discloses a vehicle-mounted snake venom processing device and a method for processing snake venom by using the same. The vehicle-mounted snake venom processing device comprises a vehicle body, a workbench and a power supply device arranged in the vehicle body, and the workbench is provided with a separation device, a drying device, a detection device and a storage device. The drying device comprises a vehicle-mounted freeze dryer and an oil-free vacuum pump, and the crude snake venom separated by the separation device is subjected to freeze drying to obtain freeze-dried snake venom powder. The detection device comprises one or more of an enzyme label instrument, an ultraviolet spectrophotometer and a moisture tester for detecting biochemical indexes of the freeze-dried snake venom powder. The storage device is used for storing the freeze-dried snake venom powder which passes the detection. The vehicle-mounted snake venom processing device has a compact structure and is convenient to move and use, is suitable for collecting and processing snake venom in the scattered snake breeding of present snake breeders, significantly shortens the snake venom processing time, effectively retains active ingredients of the snake venom, improves the medicinal value of the snake venom, and is favorable for classified storage of the snake venom through on-site detection and analysis.

The application relates to the technical field of peptide synthesis, and discloses a preparation method of a snake venom-like tripeptide, which comprises the following steps: condensing Boc-beta-Ala-OH and H-Pro-OMe, hydrolyzing the methyl ester groups to obtain Boc-beta-Ala-Pro-OH; condensing Boc-Gln-OH and benzylamine, and removing Boc to obtain H-Gln-NHBzl; then condensing Boc-beta-Ala-Pro-OH and H-Gln-NHBzl to prepare a polypeptide; and after the Gln in the polypeptide is subjected to Hofmann rearrangement, the Boc protection group in the polypeptide is removed to obtain the snake venom-like tripeptide. The preparation method adopts liquid-phase synthesis of the snake venom-like tripeptide, the synthesis route is short, the reaction condition is mild, special equipment and reagents are not needed, the operation steps are simplified, the process cost is reduced; the reaction reagents used are cheap and easy-to-obtain industrial reagents, the raw material cost is reduced; and the product purity can reach more than 99%.

The invention belongs to the technical field of medicine separation, and particularly relates to magnetic bead immobilized snake venom phospholipase A2 as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out a coupling reaction on ferroferric oxide nanoparticles and (3-aminopropyl)-triethoxysilane in an organic solvent, washing, and drying to obtain aminated magnetic beads; and activating the aminated magnetic beads with glutaraldehyde, incubating with a snake venom phospholipase A2 solution, and reacting to obtain the magnetic bead immobilized snake venom phospholipase A2. The magnetic bead immobilized snake venom phospholipase A2 prepared by the invention can be used for rapidly and accurately fishing the snake venom phospholipase A2 toxin antidote from a mixture system, and has a better application prospect in the field of snake venom antidote research.

The invention relates to the technical field of biological medicines, and relates to specific primers and a method for identifying coral snake, cobra, king cobra and snake venom source species thereof, and application of the specific primers and the method. Based on highly-conservative and specific DNA fragments contained in DNAs extracted from tissues and snake venom of bungarus multicinctus, cobra and king cobra, specific primers are respectively designed for the fragments, and the bungarus multicinctus, cobra and king cobra and snake venom source species can be accurately identified. The method can be used for identifying bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus The method is high in detection sensitivity, high in correct detection rate, free of cross reaction and simple in operation process, and has higher specificity compared with a traditional immunoassay method.

The invention provides a synthetic method of snake venom-like tetrapeptide. Specifically, the invention provides a method for preparing the snake venom-like tetrapeptide H-beta-Ala-Pro-Dab-D-Tyr-NH-Bzl in a reaction mode of combining a liquid phase and a solid phase. According to the preparation method, racemization of Pro and Dab can be avoided, the process route is stable and simpler, and the high-purity snake venom-like tetrapeptide can be conveniently prepared at lower cost.

The invention discloses a saussurea involucrata polypeptide and application thereof, and belongs to the technical field of polypeptides, the saussurea involucrata polypeptide comprises saussurea involucrata tripeptide-1, saussurea involucrata pentapeptide-4, saussurea involucrata tripeptide-5, saussurea involucrata hexapeptide-8, saussurea involucrata hexapeptide-9 or saussurea involucrata acyl snake venom tripeptide, and the saussurea involucrata polypeptide is prepared through an amide condensation method of saussurea involucrata acid. The invention has the following beneficial effects: the anti-wrinkle, firming, soothing and anti-oxidation effects of the thousand-day chrysanthemum acyl tripeptide-1, the thousand-day chrysanthemum acyl pentapeptide-4, the thousand-day chrysanthemum acyl tripeptide-5, the thousand-day chrysanthemum acyl hexapeptide-8, the thousand-day chrysanthemum acyl hexapeptide-9 or the thousand-day chrysanthemum acyl snake venom tripeptide have obvious effects on the anti-wrinkle, firming, soothing and anti-oxidation effects; particularly, the instant wrinkle removal effect is particularly outstanding.

The invention discloses a blood matrix liquid D-dimer, an FDP quality control product and a preparation method, and the preparation method comprises the following steps: adding thrombin into human mixed plasma to obtain a cross-linked fibrin clot, and carrying out washing, dissolving, inactivation and ultrafiltration treatment on the obtained cross-linked fibrin clot to obtain a D-D mother solution; the preparation method comprises the following steps: adding snake venom thrombin into human mixed plasma to obtain a non-crosslinked fibrin polymer, and carrying out washing, precipitation dissolution, inactivation and ultrafiltration treatment on the obtained non-crosslinked fibrin clot to obtain FDP mother liquor; a buffer agent, a stabilizer and a preservative are added into human serum to obtain matrix serum; the D-D mother liquor and the FDP mother liquor are respectively added into matrix serum, and the matrix serum added with the D-D mother liquor and the FDP mother liquor is filtered and frozen to obtain the D-D and FDP composite quality control product. The human matrix serum is adopted, and the difference between different detection systems is small.

The present invention relates to a pharmaceutical composition for preventing or treating rheumatoid arthritis, comprising snake venom derived from Agkistrodon piscivorus piscivorus or Naja melanoleuca, which are types of snakes, an extract thereof, or a fraction thereof; a quasi-drug composition; a food composition; a cosmetic composition; and a method of treating rheumatoid arthritis by using the composition. The snake venom derived from Agkistrodon piscivorus piscivorus or Naja melanoleuca of the present invention can improve, alleviate, or treat symptoms of rheumatoid arthritis, which is a kind of autoimmune diseases, and thus will be able to be widely used in the development of therapeutic agents for various autoimmune diseases including rheumatoid arthritis.

The invention discloses an anti-inflammatory and anti-aging cosmetic capable of being used after medical art, belongs to the field of cosmetics, and particularly relates to a liquid compound which is prepared from a utility agent, an auxiliary agent and water and can be used in the cosmetics, and the auxiliary agent further comprises a thickening agent, a humectant and a preservative. The utility agent at least comprises an active matter, the active matter comprises at least one of acetyl hexapeptide-8, oligopeptide-1, snake venom-like peptide, carnosine, palmitoyl tripeptide-1, ACE lipidosome, a bifidus yeast fermentation product and a lysate, and an active agent can also be added into the utility agent. The liquid compound obtained by the invention has the advantages of no cytotoxicity, good safety, capability of promoting cell proliferation, good anti-inflammatory effect, good anti-aging effect, no irritation to skin and good anti-inflammatory effect after medical treatment.

The invention discloses a sweet protein homotrimer as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. According to the technical scheme, the strategy of self-assembly of interchain disulfide bonds mediated by a PCA trimerization structural domain is adopted, the PCA trimerization structural domain is derived from a C-terminal fragment of snake venom serine protease, a stable homotrimer is spontaneously formed through the interchain disulfide bonds under the oxidation condition, and the stable homotrimer is used as an artificial trimerization'molecular rivet '; the PCA trimerization structural domain fragment is fused with sweet protein, controllable trimer assembly can be achieved in cells and / or outside cells, and no extra cross-linking agent is needed. Based on this, a sweet protein homotrimer is obtained from a sweet protein monomer; the sweet protein homotrimer has good thermal stability and pH tolerance, and a new technical platform is provided for industrial production of high-sweetness and high-stability functional protein.

The application provides an analgesic hemostatic agent and a preparation method thereof, and belongs to the technical field of medicines. A decellularized material is coupled with a snake venom thrombin and ibuprofen, embedded in a pH-responsive polymer to prepare pH-responsive microspheres, and added into a temperature-sensitive hydrogel together with a traditional Chinese medicine fermentation product to prepare an analgesic hemostatic agent water agent. Porous calcium carbonate microspheres with fixed solid acid are mixed with hydroxyapatite to prepare a spray analgesic hemostatic agent powder, which is used in combination with the analgesic hemostatic agent water agent to prepare the analgesic hemostatic agent. The analgesic hemostatic agent prepared by the application adopts a combination of the powder and the water agent, can better maintain the drug activity, has good hemostatic, analgesic, anti-inflammatory and antibacterial effects, and has a slow and controlled release and a long-acting effect, so that the drug application amount is reduced, the patient compliance is improved, and the analgesic hemostatic agent has a wide application prospect.

The invention discloses a rapid positioning method and system for a snake bite treatment medical institution. The method comprises the following steps: S1, acquiring real-time position information of a user through a system applet and providing first-aid information; s2, screening a medical institution containing anti-snake venom serum; s3, sorting the screened medical institutions according to distances; and S4, interactive navigation. According to the invention, the problems of non-uniform treatment resource allocation, untimely information updating, lack of targeted hospital screening function and the like in the prior art are solved, rapid, accurate and efficient emergency treatment can be realized, the fatality rate and disability rate of snake bites are reduced to the greatest extent, and the risk of snake bites is reduced. The system has the advantages of quickly and accurately positioning treatment points, updating treatment capability information of medical institutions in real time, intelligently screening the most suitable treatment institutions and improving self-rescue and mutual-rescue capabilities of the public.

The invention belongs to the technical field of biomedical cosmetology, and particularly relates to a bioactive composition with an anti-aging effect and application. The invention firstly provides a composition capable of promoting exogenous collagen to be integrated onto a collagen network. The composition is prepared from acetyl hexapeptide-8, snake venom-like peptide and PDRN. The composition can promote exogenous collagen to be integrated into an endogenous collagen network so as to achieve long-acting anti-aging and anti-wrinkle effects.

The application discloses an antibody or antigen binding fragment against agkistrodon antivenom and application thereof. The antibody or antigen binding fragment against agkistrodon antivenom comprises three heavy chain complementarity determining regions which can specifically bind to agkistrodon antivenom and the amino acid sequences of which are shown in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8; and three light chain complementarity determining regions which the amino acid sequences of which are shown in SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16. The antibody or antigen binding fragment against agkistrodon antivenom can specifically bind to agkistrodon antivenom and does not bind to five-step snake venom, vipers venom, cobra venom and silver ring snake venom, and can be used for detection of agkistrodon antivenom. The monoclonal antibody of the application has neutralization and protection effects on agkistrodon antivenom, and has important significance for assisting clinical treatment, reducing complications and improving survival rate of patients.

Novel synthetic peptide sequences based on Elapidae snake venom suitable for use as cardiotoxic rodenticides are described.

The invention discloses phospholipase A2 inhibitory proteins PLI1 and PLI2 as well as a gene for coding the phospholipase A2 inhibitory proteins PLI1 and PLI2 and application thereof, and belongs to the technical field of gene engineering. In the implementation scheme, the recombinant phospholipase A2 inhibitory protein obtained through a gene shuffling technology is described, the two phospholipase A2 inhibitory proteins PLI1 and PLI2 are screened out through a phage display technology, compared with the inhibitory protein before shuffling, the two phospholipase A2 inhibitory proteins PLI1 and PLI2 still have the good inhibitory effect and activity, and one of the two phospholipase A2 inhibitory proteins PLI1 and PLI2 is obviously superior to the inhibitory protein before shuffling. The invention also comprises polynucleotide for coding the protein and a recombinant vector containing the polynucleotide, and the protein is suitable for preparing the snake venom inhibitor, has broad spectrum and high activity, is expected to realize large-scale mass production, and has wide application prospects.

According to the present invention, it is found that a snake venom metaloproteinase SVMP (snake venom metalloproteinase) from a snake belonging to the family Viperidae, the family Elapidae or the like decomposes Aβ and cuts the Aβ at an α-position to suppress the formation of Aβ fibrils. When a protein containing a metaloproteinase domain of SVMP is administered or a gene for the protein is introduced and is expressed in the brain, the formation of Aβ fibrils can be suppressed and consequently Alzheimer's disease can be treated.

The present application belongs to the technical field of cosmetic product ingredient identification, and particularly relates to a method for identifying and detecting counterfeit ingredients of counterfeit botulinum toxin cosmetic products. The present application prepares a test sample into a test solution, detects it according to high performance liquid chromatography mass spectrometry, takes telencephalin, acetyl hexapeptide-1 and snake venom-like tripeptide as key indicators for distinguishing counterfeit ingredients of counterfeit botulinum toxin products, and ensures the safety and effectiveness of the botulinum toxin products. The present application only needs to use standard control samples of counterfeit ingredients when the method is established for the first time, and does not need to use control samples in the subsequent routine detection process to accurately determine whether the sample is counterfeit. Moreover, the present application first establishes a method for quantitatively analyzing telencephalin by using liquid chromatography-tandem mass spectrometry. The present application is simple in operation, good in repeatability, high in sensitivity, fast in separation speed, high in detection flux, reliable in results, and effectively ensures the safety and effectiveness of the botulinum toxin products.

The invention discloses a whitening, spot-fading and repairing type anti-aging glass repairing agent with a high-pressure micro-jet nanoscale transdermal absorption function. Comprising the following raw materials in parts by weight: 5-8 parts of vitriol, 3-5 parts of modified shell powder, 1-3 parts of peptides, 1-3 parts of glabridin, 2-3 parts of modified sodium hyaluronate, 2-3 parts of sodium polyglutamate, 4-6 parts of glycerol, 2-3 parts of ceramide NP, 2-3 parts of phosphatidylcholine and 7-10 parts of water. Wherein the peptide substance is prepared from carnosine, snake venom and argireline according to the weight ratio of 1: 1: 1. According to the anti-aging glass repairing agent, ceramide NP and phosphatidylcholine are adopted to be matched with glycerin, meanwhile, the raw materials such as the glass repairing agent, the modified shell powder, the peptides, the glabridin and the modified sodium hyaluronate are matched with the sodium polyglutamate, and the raw materials are blended, coordinated and jointly synergized, so that the obtained product realizes the coordination and optimization of whitening and anti-aging; and the transdermal absorption efficiency of the product is remarkable.