30 results about "Snake venom" patented technology

The invention relates to the technical field of detection kits, in particular to a blood coagulation reaction linked lupus anticoagulant fluorescence detection kit and application thereof, the kit contains a screening-confirmation integrated reagent, a calibration product, a quality control product and an anticoagulation interference inhibitor; the integrated reagent is divided into a reagent A and a reagent B, the reagent A contains a buffer solution, recombinant snake venom thrombin, composite phospholipid, a fluorescent substrate and other components, and the reagent B contains prothrombin, calcium ions, high-concentration composite phospholipid and other components; the composite phospholipid system is formed according to a specific proportion and modifies beta2-glycoprotein I, the fluorescence enhancement-stabilization system is formed by matching a Schiff base compound with a stabilizer, the calibrator is a series of lupus anticoagulant standard substances, and the quality control substance comprises simulants with different concentrations and negative control. The anti-interference capability is high, and the influence of anti-coagulant drugs and enzyme source fluctuation is reduced; the signal amplification effect is obvious, the detection sensitivity is high and the reagent stability is good; the operation is simple and convenient, screening and confirmation are integrally completed, and a conventional fluorescence detector is adapted.

The present application relates to the beer brewing technical field, especially a kind of venomous beer brewing process, including the following steps: raw material preparation and pretreatment;Saccharification;Filtering;Boiling;Rotary sedimentation;Fermentation;After mature wine, under low pressure 8.986kPa pressure, reduce the boiling point of water, ensure that the boiling point is lower than 25 degrees Celsius, concentrate wine, concentrate to 15 liters or less per 100 liters, carry out oak barrel barrel;Oak barrel barrel.The product is soft in taste, no spicy residue, alcohol burning sensation controllable;Flavor levels are rich, with fruit, flower, nut and other complex aroma;Process parameters are accurate, without low-temperature freezing equipment, small and medium-sized enterprises can replicate and the quality of stable venomous beer brewing process.

The application belongs to the technical field of biotechnology, and particularly relates to a kind of snake venom polypeptide and application thereof.The snake venom polypeptide is polypeptide DACU-1, polypeptide DACU-2 or polypeptide DACU-3.The application provides a kind of new snake venom polypeptide, including three polypeptides, and the three polypeptides all have anti-platelet aggregation activity, and have the activity of inhibiting platelet aggregation under the stimulation of ADP, arachidonic acid, collagen, thrombin and other various activators, and have the potential to be developed into anti-platelet drugs, functional foods and health products.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition and application thereof. The composition is prepared from a white birch bark extract, snake venom peptide and Ectoine. The white birch bark extract promotes abnormal protein removal by activating an intracellular protein ubiquitination system, and improves the skin metabolism capability; the snake venom peptide slows down expression muscle contraction through a nerve regulation mechanism, and dynamic wrinkles are effectively faded; ectoin enhances cell membrane stability, protein protection and skin stress adaptive capacity, and under the synergistic effect of Ectoin, Ectoin and skin stress adaptive capacity, the comprehensive anti-aging effects of resisting wrinkles, tightening, relieving, brightening skin color and the like can be remarkably improved.

1. Name of the product in this design: Snake Venom Detector (VENOMSCAN). 2. Purpose of this design: This design is used for snake venom detection. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application discloses a vehicle-mounted snake venom processing device and a method for processing snake venom by using the same. The vehicle-mounted snake venom processing device comprises a vehicle body, a workbench and a power supply device arranged in the vehicle body, and the workbench is provided with a separation device, a drying device, a detection device and a storage device. The drying device comprises a vehicle-mounted freeze dryer and an oil-free vacuum pump, and the crude snake venom separated by the separation device is subjected to freeze drying to obtain freeze-dried snake venom powder. The detection device comprises one or more of an enzyme label instrument, an ultraviolet spectrophotometer and a moisture tester for detecting biochemical indexes of the freeze-dried snake venom powder. The storage device is used for storing the freeze-dried snake venom powder which passes the detection. The vehicle-mounted snake venom processing device has a compact structure and is convenient to move and use, is suitable for collecting and processing snake venom in the scattered snake breeding of present snake breeders, significantly shortens the snake venom processing time, effectively retains active ingredients of the snake venom, improves the medicinal value of the snake venom, and is favorable for classified storage of the snake venom through on-site detection and analysis.

The application relates to the technical field of peptide synthesis, and discloses a preparation method of a snake venom-like tripeptide, which comprises the following steps: condensing Boc-beta-Ala-OH and H-Pro-OMe, hydrolyzing the methyl ester groups to obtain Boc-beta-Ala-Pro-OH; condensing Boc-Gln-OH and benzylamine, and removing Boc to obtain H-Gln-NHBzl; then condensing Boc-beta-Ala-Pro-OH and H-Gln-NHBzl to prepare a polypeptide; and after the Gln in the polypeptide is subjected to Hofmann rearrangement, the Boc protection group in the polypeptide is removed to obtain the snake venom-like tripeptide. The preparation method adopts liquid-phase synthesis of the snake venom-like tripeptide, the synthesis route is short, the reaction condition is mild, special equipment and reagents are not needed, the operation steps are simplified, the process cost is reduced; the reaction reagents used are cheap and easy-to-obtain industrial reagents, the raw material cost is reduced; and the product purity can reach more than 99%.

The invention relates to the technical field of biological medicines, and relates to specific primers and a method for identifying coral snake, cobra, king cobra and snake venom source species thereof, and application of the specific primers and the method. Based on highly-conservative and specific DNA fragments contained in DNAs extracted from tissues and snake venom of bungarus multicinctus, cobra and king cobra, specific primers are respectively designed for the fragments, and the bungarus multicinctus, cobra and king cobra and snake venom source species can be accurately identified. The method can be used for identifying bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus The method is high in detection sensitivity, high in correct detection rate, free of cross reaction and simple in operation process, and has higher specificity compared with a traditional immunoassay method.

The invention discloses a sweet protein homotrimer as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. According to the technical scheme, the strategy of self-assembly of interchain disulfide bonds mediated by a PCA trimerization structural domain is adopted, the PCA trimerization structural domain is derived from a C-terminal fragment of snake venom serine protease, a stable homotrimer is spontaneously formed through the interchain disulfide bonds under the oxidation condition, and the stable homotrimer is used as an artificial trimerization'molecular rivet '; the PCA trimerization structural domain fragment is fused with sweet protein, controllable trimer assembly can be achieved in cells and / or outside cells, and no extra cross-linking agent is needed. Based on this, a sweet protein homotrimer is obtained from a sweet protein monomer; the sweet protein homotrimer has good thermal stability and pH tolerance, and a new technical platform is provided for industrial production of high-sweetness and high-stability functional protein.

The invention belongs to the technical field of biomedical cosmetology, and particularly relates to a bioactive composition with an anti-aging effect and application. The invention firstly provides a composition capable of promoting exogenous collagen to be integrated onto a collagen network. The composition is prepared from acetyl hexapeptide-8, snake venom-like peptide and PDRN. The composition can promote exogenous collagen to be integrated into an endogenous collagen network so as to achieve long-acting anti-aging and anti-wrinkle effects.

The application discloses an antibody or antigen binding fragment against agkistrodon antivenom and application thereof. The antibody or antigen binding fragment against agkistrodon antivenom comprises three heavy chain complementarity determining regions which can specifically bind to agkistrodon antivenom and the amino acid sequences of which are shown in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8; and three light chain complementarity determining regions which the amino acid sequences of which are shown in SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16. The antibody or antigen binding fragment against agkistrodon antivenom can specifically bind to agkistrodon antivenom and does not bind to five-step snake venom, vipers venom, cobra venom and silver ring snake venom, and can be used for detection of agkistrodon antivenom. The monoclonal antibody of the application has neutralization and protection effects on agkistrodon antivenom, and has important significance for assisting clinical treatment, reducing complications and improving survival rate of patients.

The present application belongs to the technical field of cosmetic product ingredient identification, and particularly relates to a method for identifying and detecting counterfeit ingredients of counterfeit botulinum toxin cosmetic products. The present application prepares a test sample into a test solution, detects it according to high performance liquid chromatography mass spectrometry, takes telencephalin, acetyl hexapeptide-1 and snake venom-like tripeptide as key indicators for distinguishing counterfeit ingredients of counterfeit botulinum toxin products, and ensures the safety and effectiveness of the botulinum toxin products. The present application only needs to use standard control samples of counterfeit ingredients when the method is established for the first time, and does not need to use control samples in the subsequent routine detection process to accurately determine whether the sample is counterfeit. Moreover, the present application first establishes a method for quantitatively analyzing telencephalin by using liquid chromatography-tandem mass spectrometry. The present application is simple in operation, good in repeatability, high in sensitivity, fast in separation speed, high in detection flux, reliable in results, and effectively ensures the safety and effectiveness of the botulinum toxin products.

The invention discloses a whitening, spot-fading and repairing type anti-aging glass repairing agent with a high-pressure micro-jet nanoscale transdermal absorption function. Comprising the following raw materials in parts by weight: 5-8 parts of vitriol, 3-5 parts of modified shell powder, 1-3 parts of peptides, 1-3 parts of glabridin, 2-3 parts of modified sodium hyaluronate, 2-3 parts of sodium polyglutamate, 4-6 parts of glycerol, 2-3 parts of ceramide NP, 2-3 parts of phosphatidylcholine and 7-10 parts of water. Wherein the peptide substance is prepared from carnosine, snake venom and argireline according to the weight ratio of 1: 1: 1. According to the anti-aging glass repairing agent, ceramide NP and phosphatidylcholine are adopted to be matched with glycerin, meanwhile, the raw materials such as the glass repairing agent, the modified shell powder, the peptides, the glabridin and the modified sodium hyaluronate are matched with the sodium polyglutamate, and the raw materials are blended, coordinated and jointly synergized, so that the obtained product realizes the coordination and optimization of whitening and anti-aging; and the transdermal absorption efficiency of the product is remarkable.

The invention discloses an insect-proof and high-temperature-resistant photovoltaic cable, which is sequentially provided with a wear-resistant layer, an armor layer, an anti-biting layer, a fireproof layer and a wire from the outer layer to the inner layer, and is characterized in that the thickness of the anti-biting layer is at least one sixth of the overall thickness of the photovoltaic cable; the anti-biting layer is prepared from the following components in parts by weight: 30 to 40 parts of phosphorus-silicon resin, 4 to 8 parts of white carbon black, 20 to 30 parts of acrylonitrile-styrene copolymer, 10 to 18 parts of ethylene propylene diene monomer, 5 to 15 parts of polypropylene, 1 to 2 parts of cross-linking agent and 10 to 20 parts of insect-proof medicinal powder; the insect-proof medicine powder is prepared from 5-8 parts of salvia officinalis, 6-10 parts of verbena, 4-8 parts of pepper, 2-4 parts of radix angelicae, 3-6 parts of cynanchum komarovii and 2-4 parts of mint. According to the invention, insect-proof powder in the anti-bite layer has a certain smell and can prevent rats, termites and the like from biting the cable, a certain amount of heat can be generated when the wires are electrified, the heat can be transmitted to the anti-bite layer through the heat conduction columns, the armor layer can absorb a certain amount of solar heat, and the anti-bite layer can absorb the solar energy under the action of the heat on the two sides of the anti-bite layer. The smell emission of the insect-proof medicine powder is facilitated.

The invention belongs to the technical field of biology, and discloses a composition for improving skin inflammation and aging as well as a preparation method and application thereof. The composition is prepared from an exosome, sodium hyaluronate, poly (deoxyribonucleotide) and a snake venom-like peptide. The composition has an unexpected synergistic effect in the aspects of inhibiting expression of IL-6 and reducing the ROS level, the composition is simple in formula, and the composition is matched with corresponding processes and auxiliary materials to be prepared into a required dosage form, so that the problems of skin inflammation and aging can be effectively solved.

The invention relates to a combination preparation for use in a method for the treatment and / or prophylaxis of snakebites, wherein the combination preparation comprises one or more compositions which - Varespladib, methylvarespladib, hydroxy-varespladib, hydroxy-methylvarespladib, methoxy-varespladib, methoxy-methylvarespladib and / or corresponding salts, esters, amides or solvates and - contains a dimercapto chelating agent. The ingredients of the combination preparation complement each other advantageously for the effective treatment or prophylaxis of snakebites while keeping side effects to a minimum.

Provided is an monoclonal antibody for treating envenomation of snake venom of Russell's viper. Also provided is an application of the monoclonal antibodies thereof, including a method for treating Russell's viper envenomation using the monoclonal antibodies.

The invention discloses a composition containing nonapeptide-1, acetyl hexapeptide-8 and arginine lysine as well as a preparation method and application of the composition. The technical problem that an existing product is poor in synergistic interaction is mainly solved. The composition is composed of a core active component, an auxiliary active component and an auxiliary material, the core active component comprises nonapeptide-1, acetyl hexapeptide-8 and a mixture of arginine and lysine in a specific ratio, and the auxiliary active component is selected from snake venom-like peptide or a chrysanthemum extract. Compared with the prior art, by precisely limiting the ratio range of core active ingredients (8-18 parts of nonapeptide-1, 25-35 parts of acetyl hexapeptide-8, 45-55 parts of an arginine-lysine mixture, and the weight ratio of arginine to lysine is 1.5: 1-2.5: 1), the synergistic interaction of three effects of whitening, wrinkle resistance and repairing is creatively realized.

The invention discloses a vector for expressing snake venom thrombin-like bacteria, yeast engineering bacteria and a preparation method, and belongs to the technical field of gene engineering. Based on pPIC9K, pAO815 alpha, thrombin-like snake venom agkihpin and a pichia pastoris expression system, the co-expression copy number of the agkihpin gene and an auxiliary factor is optimized, the copy number of an auxiliary gene Bmh2 is adjusted, and culture parameters such as the initial pH of a culture medium, the methanol addition amount, the induction time, the induction temperature and an auxiliary agent are improved by the system, so that the expression level of the agkihpin is effectively improved, and the expression level of the agkihpin is improved. And a solid foundation is laid for agkihpin industrial application of the thrombin-like snake venom.

The present application relates to the field of cosmetic technology, in particular to a soothing and repairing composition, a skin care product and a preparation method thereof, wherein the composition comprises DNA sodium, a snake venom-like peptide, hydrolyzed silk fibroin, a living particle factor and an ampelopsis grossedentata extract. By controlling the amount of DNA sodium, a snake venom-like peptide, hydrolyzed silk fibroin, a living particle factor and an ampelopsis grossedentata extract in the skin care product, the prepared essence cream, essence liquid and gel have excellent soothing, repairing and anti-wrinkle effects, and meet the use requirements of sensitive skin groups.

The invention discloses a remodeling active peptide and a preparation method thereof, and belongs to the technical field of active peptides. Comprising the following components in parts by weight: 2-4 parts of acetyl octapeptide-3, 2-3 parts of acetyl hexapeptide-8, 2-3 parts of snake venom-like peptide, 3-5 parts of palmitoyl tripeptide-1, 3-5 parts of palmitoyl tetrapeptide-7, 3-5 parts of palmitoyl hexapeptide-12, 1-2 parts of tripeptide-1, 5-10 parts of a traditional Chinese medicine extract composition, 2-3 parts of white honey, 2-3 parts of walnut kernel oil, 3-5 parts of a cubilose extract and 1-2 parts of vitamin E, 2-3 parts of hyaluronic acid, 1-2 parts of ceramide and the balance of rose water. According to the invention, the designed traditional Chinese medicine extract composition can synergistically enhance skin repair, metabolism and circulation functions based on compatibility, forms a comprehensive action network for tonifying middle-Jiao and Qi, activating blood and nourishing skin, harmonizing and removing turbidity and dredging meridians and collaterals, and complements with a molecular signal mechanism of active peptides, so that the skin is improved in a cell layer, a corium layer and a barrier layer.

The invention relates to the technical field of skin care products, in particular to a ginseng oligopeptide-containing polypeptide composite solution firming and anti-wrinkle composition which comprises ginseng oligopeptide and a polypeptide composite solution, and the polypeptide composite solution comprises acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9 and acetyl dipeptide-1. Through the cooperation of the polypeptide and the ginseng oligopeptide, collagen generation is enhanced, skin aging inducements are reduced by means of the anti-aging, anti-oxidation and anti-inflammatory characteristics of the ginseng oligopeptide, and more comprehensive anti-wrinkle and firming effects are achieved.

The invention relates to a snake venom-like tripeptide derivative as well as a preparation process and application thereof, and belongs to the technical field of polypeptide synthesis. The snake venom-like tripeptide derivative disclosed by the invention is mainly citronellyl snake venom tripeptide or bis-citronellyl snake venom tripeptide. The snake venom-like tripeptide derivative provided by the invention can significantly inhibit release of acetylcholine in cells, and has a significant instant anti-wrinkle effect.

The application provides a method for treating snake venom by adenosine or a pharmaceutical composition containing adenosine, which comprises administering an effective dose of adenosine or a pharmaceutical composition containing adenosine. The adenosine or the pharmaceutical composition containing adenosine of the application can be applied to the first-aid treatment of snake bites in primary hospitals, and is expected to achieve remarkable curative effects.

A system (100) for integrated animal and bird care and information is disclosed. A receiving module (120) receive user information for registering a user. An information provider module (125) provides information about a plurality of veterinary hospitals, doctors, and compounders, provide information about the hospitals that offer rabies vaccinations, antivenom for specific snake bites, and medication for animal bites, provide information about snake catchers and forest department staff, provide information about the medication of the animals and birds. A complaint module (130) enables the user to raise a complaint request about animal issues. A notification module (135) notifies the user about the competition of the animal, type of animal competition, list of participants, and win or loss history of animal and owner. A zoo module (140) provides a plurality of information about the zoo. A sale module (145) provides information regarding the sale of animals and birds to the user.

The invention discloses a nanofiber protective film integrated with a fluorescence sensing function as well as a preparation method and application thereof, and belongs to the technical field of functional materials and biosensing. According to the protective film, thermoplastic polyurethane (TPU) serves as a base material, hydrophobic fluorescent dye is embedded in fibers through an electrostatic spinning technology, hydrophilic fluorescent dye is immobilized on the surfaces of the fibers through covalent cross-linking, and an inner and outer double-layer dye loading structure is formed. The structure can generate specific fluorescence response when the snake venom protein is contacted, and multi-dimensional fluorescent fingerprints are formed. The invention also provides a toxin protective garment comprising the protective film, and the toxin protective garment integrates a signal acquisition module, a signal processing module and an early warning module, so that rapid identification and real-time alarm of toxin types can be realized. The invention has the advantages of excellent protection performance, high environmental adaptability and active sensing capability, and is suitable for special operation and outdoor safety protection.

This invention belongs to the field of biotechnology and discloses a detectable and therapeutic antibody against the venom of the Chinese cobra and its uses. This invention provides an antibody or its antigen-binding fragment that can target and recognize different key toxin components or antigenic epitopes in venom, exhibiting excellent diagnostic specificity and therapeutic and preventative capabilities. Utilizing the high specificity of this antibody or its antigen-binding fragment in recognizing snake venom antigens, it can be developed into a rapid detection kit (such as a lateral flow immunochromatographic test strip) for rapid identification of the biting snake species and detection of the presence of specific snake venom in the environment or sample, providing crucial technical support for precise treatment and on-site diagnosis. It can also be used directly as a neutralizing agent for emergency treatment after snakebite. Through injection, the antibody can specifically neutralize the snake venom toxins entering the body, blocking its toxic effects and providing a novel and specific treatment method for clinical care.

The application relates to the field of biological medicine, in particular to a detection method and application of ophidiotoxin and vivero toad venom. (1) Antibodies obtained by immunizing a mixture of SVSP chimeras and Disintegrin chimeras and two kinds of antigens of 3FT chimeras can effectively identify ophidiotoxin and vivero toad venom; (2) Antibodies obtained by immunizing two kinds of antigens of multicinctus 3FT chimeras and Atra 3FT chimeras can effectively identify ophidiotoxin and silver ring snake venom; (3) The cation exchange chromatography method is used to enrich the snake venom in the sample, and the sensitivity of the to-be-detected snake venom in blood and tissue fluid is improved; (4) Standard samples of cobra and silver ring snake venom are established for the first time. Compared with the prior art, the method has the effects of identifying vivero toad venom and ophidiotoxin by searching for difference proteins, predicting linear antigen epitopes, removing the same and highly similar linear antigen epitopes and serially recombinantly expressing the linear antigen epitopes.

The invention belongs to the field of cosmetic compositions, and particularly relates to a structure-repairing intelligent polypeptide anti-aging composition as well as a preparation method and application thereof. The composition comprises repair polypeptide, efficacy optimization polypeptide and a system synergistic composition, and the mass ratio of the repair polypeptide to the efficacy optimization polypeptide to the system synergistic composition is (1-8): (3-16): (3.5-13). The repairing polypeptide comprises acetyl hexapeptide-8, palmitoyl pentapeptide-5, snake venom peptide and arginine / lysine polypeptide; the efficacy optimization polypeptide is acetyl tetrapeptide-2 and palmitoyl tripeptide-5; the system synergistic composition is prepared from nicotinamide, hydroxypropyl tetrahydropyrantriol and a Thermus thermophilus fermentation product. Through multi-channel synergistic anti-aging, the problems of single efficacy, poor synergism and low bioavailability in the prior art are solved, tests show that the effects of reducing wrinkles, improving elasticity and the like are remarkably superior to those of existing products, and the composition can be used for preparing cosmetics.

This invention discloses an adaptive low-temperature snake venom collection device and method. The adaptive low-temperature snake venom collection device includes an adaptive phase change temperature-controlled venom extraction head, a miniature power-free constant temperature energy storage chamber, a viscosity and negative pressure collaborative closed-loop control system, and an intelligent activity early warning module. The adaptive phase change temperature-controlled venom extraction head is equipped with a multi-layer gradient biomimetic membrane and solid-liquid phase change material layers, utilizing the latent heat of phase change to maintain the internal cavity temperature at 1±1℃. The miniature power-free constant temperature energy storage chamber combines multi-level insulation layers and a phase change energy storage structure to achieve passive temporary storage. The viscosity and negative pressure collaborative closed-loop control system calculates the venom viscosity based on pipeline pressure parameters and drive motor current parameters, and dynamically adjusts the absolute value of the output negative pressure of the negative pressure generator based on the venom viscosity. The intelligent activity early warning module simultaneously detects the pH value and conductivity of the flowing venom to execute abnormal early warning. This invention achieves constant temperature protection without external power supply, relies on closed-loop negative pressure to prevent venom residue, and completes real-time denaturation identification.