57 results about "Snake venom" patented technology

The method is mainly applied to the technical field of deep learning. The invention discloses an intelligent diagnosis method, device and equipment for snake venom poisoning and a medium, and the method comprises the steps: obtaining multi-modal data which comprises a wound image, a symptom text and a blood parameter; performing feature extraction processing on the wound image, the symptom text and the blood parameters to obtain image features corresponding to the wound image, semantic features corresponding to the symptom text and high-dimensional features corresponding to the blood parameters; performing alignment and fusion processing on the image features, the semantic features and the high-dimensional features through a cross-modal attention mechanism to generate fusion features; after the fusion features are input into a preset multi-task classification model, classification is carried out in a snake species label space and a toxicity category space according to the fusion features, a poisoning grade score is determined, and a diagnosis report is generated based on the poisoning grade score. According to the invention, high-precision, rapid and intelligent diagnosis of snake venom poisoning can be realized.

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

The invention discloses bee venom-containing skin essence with small irritation and a preparation method of the bee venom-containing skin essence. The skin essence is prepared from PLGA-PEG / liposome nanoparticles, a temperature-sensitive gel matrix, a protective agent, an antioxidant, a stabilizer and ultrapure water, the preparation method comprises the following steps: preparing the PLGA-PEG / liposome nanoparticles, preparing a poloxamer 407 solution, resuspending the nanoparticles, filling and storing. According to the invention, the melittin and the snake venom-like peptide have a synergistic effect on skin wrinkle resistance, so that the skin care effect is improved; pLGA-PEG / liposome nanoparticles are matched with a poloxamer solution to construct a dual sustained-release drug system, so that the irritation of drugs to skin is reduced, the lasting effect of the drug effect is improved, and the obtained skin essence has excellent moisturizing, whitening, anti-wrinkle and antioxidant capacities and is small in irritation.

The invention relates to the technical field of detection kits, in particular to a blood coagulation reaction linked lupus anticoagulant fluorescence detection kit and application thereof, the kit contains a screening-confirmation integrated reagent, a calibration product, a quality control product and an anticoagulation interference inhibitor; the integrated reagent is divided into a reagent A and a reagent B, the reagent A contains a buffer solution, recombinant snake venom thrombin, composite phospholipid, a fluorescent substrate and other components, and the reagent B contains prothrombin, calcium ions, high-concentration composite phospholipid and other components; the composite phospholipid system is formed according to a specific proportion and modifies beta2-glycoprotein I, the fluorescence enhancement-stabilization system is formed by matching a Schiff base compound with a stabilizer, the calibrator is a series of lupus anticoagulant standard substances, and the quality control substance comprises simulants with different concentrations and negative control. The anti-interference capability is high, and the influence of anti-coagulant drugs and enzyme source fluctuation is reduced; the signal amplification effect is obvious, the detection sensitivity is high and the reagent stability is good; the operation is simple and convenient, screening and confirmation are integrally completed, and a conventional fluorescence detector is adapted.

The present application relates to the beer brewing technical field, especially a kind of venomous beer brewing process, including the following steps: raw material preparation and pretreatment;Saccharification;Filtering;Boiling;Rotary sedimentation;Fermentation;After mature wine, under low pressure 8.986kPa pressure, reduce the boiling point of water, ensure that the boiling point is lower than 25 degrees Celsius, concentrate wine, concentrate to 15 liters or less per 100 liters, carry out oak barrel barrel;Oak barrel barrel.The product is soft in taste, no spicy residue, alcohol burning sensation controllable;Flavor levels are rich, with fruit, flower, nut and other complex aroma;Process parameters are accurate, without low-temperature freezing equipment, small and medium-sized enterprises can replicate and the quality of stable venomous beer brewing process.

The application belongs to the technical field of biotechnology, and particularly relates to a kind of snake venom polypeptide and application thereof.The snake venom polypeptide is polypeptide DACU-1, polypeptide DACU-2 or polypeptide DACU-3.The application provides a kind of new snake venom polypeptide, including three polypeptides, and the three polypeptides all have anti-platelet aggregation activity, and have the activity of inhibiting platelet aggregation under the stimulation of ADP, arachidonic acid, collagen, thrombin and other various activators, and have the potential to be developed into anti-platelet drugs, functional foods and health products.

The invention relates to the technical field of in-vitro diagnosis, in particular to a lupus anticoagulant detection kit based on recombinant snake venom enzyme and application of the lupus anticoagulant detection kit. The probe comprises a recombinant snake venom thrombin-like enzyme, a phospholipid-quantum dot conjugate, calcium ions, sodium citrate, trehalose, polyethylene glycol, L-histidine, EDTA-2Na, a Tris-HCl buffer solution, a nanogold-carbon nanotube composite probe, a graphene oxide-chitosan composite material, a fluorescence enhancer and a stabilizer. The kit disclosed by the invention is high in detection sensitivity, strong in matrix interference resistance, excellent in recombinant snake venom enzyme stability and good in batch-to-batch consistency, the detection specificity can be accurately verified, and the problem that the performance of a traditional kit is poor is solved.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition and application thereof. The composition is prepared from a white birch bark extract, snake venom peptide and Ectoine. The white birch bark extract promotes abnormal protein removal by activating an intracellular protein ubiquitination system, and improves the skin metabolism capability; the snake venom peptide slows down expression muscle contraction through a nerve regulation mechanism, and dynamic wrinkles are effectively faded; ectoin enhances cell membrane stability, protein protection and skin stress adaptive capacity, and under the synergistic effect of Ectoin, Ectoin and skin stress adaptive capacity, the comprehensive anti-aging effects of resisting wrinkles, tightening, relieving, brightening skin color and the like can be remarkably improved.

The invention discloses a whitening and anti-aging mask formula based on dogwood. The whitening and anti-aging mask formula based on dogwood is prepared from the following raw materials in parts by mass: 5 to 15 parts of dogwood extract, 2 to 8 parts of vitamin C powder, 1 to 3 parts of polyphenol compound, 1 to 5 parts of glycerinum, 2 to 4 parts of hyaluronic acid, 3 to 9 parts of plant essence, 2 to 6 parts of vegetable oil, 4 to 12 parts of seaweed extract and 1 to 3 parts of polyether-201. 1-3 parts of nerve growth factors, 2-6 parts of DNA (deoxyribonucleic acid) repair enzyme, 0.1-0.3 part of snake venom essence, 1-3 parts of plant stem cells and 20-30 parts of deionized water, components in the snake venom essence can stimulate synthesis of collagen and increase skin elasticity, so that wrinkles and fine wrinkles are reduced, the skin looks younger and tighter, certain antioxidant capacity is achieved, and the skin care effect is good. Free radicals can be neutralized, the damage of environmental pollution and ultraviolet radiation to the skin is reduced, and the aging process of the skin is delayed.

1. Name of the product in this design: Snake Venom Detector (VENOMSCAN). 2. Purpose of this design: This design is used for snake venom detection. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition containing DNA sodium and application of the anti-aging composition. The composition is prepared from DNA sodium, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E and sodium hyaluronate. According to the emulsion disclosed by the invention, various active ingredients such as the DNA sodium, the snake venom tripeptide, the elastin peptide, the low-molecular collagen peptide, the nicotinamide, the alpha-arbutin and the purple blue bletilla striata bulb fermentation liquor are organically combined, so that multiple anti-aging and whitening effects of resisting oxidation, inhibiting melanin synthesis, promoting collagen and elastin generation, improving uneven skin color and the like can be synergistically realized. Purple blue bletilla striata bulbs are subjected to lactobacillus-candida compound fermentation, the product does not contain coarse particles after refined filtration treatment, is clarified, is stable for a long time after being added into an emulsion system, and is not easy to separate out and precipitate or cause system layering. The emulsion is light in structure, not sticky and greasy, endows skin with soft and moist touch feeling, and is suitable for various skin types including sensitive skin, and active ingredients are easy to permeate and absorb.

The application discloses a vehicle-mounted snake venom processing device and a method for processing snake venom by using the same. The vehicle-mounted snake venom processing device comprises a vehicle body, a workbench and a power supply device arranged in the vehicle body, and the workbench is provided with a separation device, a drying device, a detection device and a storage device. The drying device comprises a vehicle-mounted freeze dryer and an oil-free vacuum pump, and the crude snake venom separated by the separation device is subjected to freeze drying to obtain freeze-dried snake venom powder. The detection device comprises one or more of an enzyme label instrument, an ultraviolet spectrophotometer and a moisture tester for detecting biochemical indexes of the freeze-dried snake venom powder. The storage device is used for storing the freeze-dried snake venom powder which passes the detection. The vehicle-mounted snake venom processing device has a compact structure and is convenient to move and use, is suitable for collecting and processing snake venom in the scattered snake breeding of present snake breeders, significantly shortens the snake venom processing time, effectively retains active ingredients of the snake venom, improves the medicinal value of the snake venom, and is favorable for classified storage of the snake venom through on-site detection and analysis.

The application relates to the technical field of peptide synthesis, and discloses a preparation method of a snake venom-like tripeptide, which comprises the following steps: condensing Boc-beta-Ala-OH and H-Pro-OMe, hydrolyzing the methyl ester groups to obtain Boc-beta-Ala-Pro-OH; condensing Boc-Gln-OH and benzylamine, and removing Boc to obtain H-Gln-NHBzl; then condensing Boc-beta-Ala-Pro-OH and H-Gln-NHBzl to prepare a polypeptide; and after the Gln in the polypeptide is subjected to Hofmann rearrangement, the Boc protection group in the polypeptide is removed to obtain the snake venom-like tripeptide. The preparation method adopts liquid-phase synthesis of the snake venom-like tripeptide, the synthesis route is short, the reaction condition is mild, special equipment and reagents are not needed, the operation steps are simplified, the process cost is reduced; the reaction reagents used are cheap and easy-to-obtain industrial reagents, the raw material cost is reduced; and the product purity can reach more than 99%.

The invention relates to the technical field of biological medicines, and relates to specific primers and a method for identifying coral snake, cobra, king cobra and snake venom source species thereof, and application of the specific primers and the method. Based on highly-conservative and specific DNA fragments contained in DNAs extracted from tissues and snake venom of bungarus multicinctus, cobra and king cobra, specific primers are respectively designed for the fragments, and the bungarus multicinctus, cobra and king cobra and snake venom source species can be accurately identified. The method can be used for identifying bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus, cobra, king cobra and snake venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus and cobra venom source species of bungarus multicinctus The method is high in detection sensitivity, high in correct detection rate, free of cross reaction and simple in operation process, and has higher specificity compared with a traditional immunoassay method.

The invention discloses a sweet protein homotrimer as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. According to the technical scheme, the strategy of self-assembly of interchain disulfide bonds mediated by a PCA trimerization structural domain is adopted, the PCA trimerization structural domain is derived from a C-terminal fragment of snake venom serine protease, a stable homotrimer is spontaneously formed through the interchain disulfide bonds under the oxidation condition, and the stable homotrimer is used as an artificial trimerization'molecular rivet '; the PCA trimerization structural domain fragment is fused with sweet protein, controllable trimer assembly can be achieved in cells and / or outside cells, and no extra cross-linking agent is needed. Based on this, a sweet protein homotrimer is obtained from a sweet protein monomer; the sweet protein homotrimer has good thermal stability and pH tolerance, and a new technical platform is provided for industrial production of high-sweetness and high-stability functional protein.

The application provides an analgesic hemostatic agent and a preparation method thereof, and belongs to the technical field of medicines. A decellularized material is coupled with a snake venom thrombin and ibuprofen, embedded in a pH-responsive polymer to prepare pH-responsive microspheres, and added into a temperature-sensitive hydrogel together with a traditional Chinese medicine fermentation product to prepare an analgesic hemostatic agent water agent. Porous calcium carbonate microspheres with fixed solid acid are mixed with hydroxyapatite to prepare a spray analgesic hemostatic agent powder, which is used in combination with the analgesic hemostatic agent water agent to prepare the analgesic hemostatic agent. The analgesic hemostatic agent prepared by the application adopts a combination of the powder and the water agent, can better maintain the drug activity, has good hemostatic, analgesic, anti-inflammatory and antibacterial effects, and has a slow and controlled release and a long-acting effect, so that the drug application amount is reduced, the patient compliance is improved, and the analgesic hemostatic agent has a wide application prospect.

The invention belongs to the technical field of biomedical cosmetology, and particularly relates to a bioactive composition with an anti-aging effect and application. The invention firstly provides a composition capable of promoting exogenous collagen to be integrated onto a collagen network. The composition is prepared from acetyl hexapeptide-8, snake venom-like peptide and PDRN. The composition can promote exogenous collagen to be integrated into an endogenous collagen network so as to achieve long-acting anti-aging and anti-wrinkle effects.

The application discloses an antibody or antigen binding fragment against agkistrodon antivenom and application thereof. The antibody or antigen binding fragment against agkistrodon antivenom comprises three heavy chain complementarity determining regions which can specifically bind to agkistrodon antivenom and the amino acid sequences of which are shown in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8; and three light chain complementarity determining regions which the amino acid sequences of which are shown in SEQ ID NO:12, SEQ ID NO:14 and SEQ ID NO:16. The antibody or antigen binding fragment against agkistrodon antivenom can specifically bind to agkistrodon antivenom and does not bind to five-step snake venom, vipers venom, cobra venom and silver ring snake venom, and can be used for detection of agkistrodon antivenom. The monoclonal antibody of the application has neutralization and protection effects on agkistrodon antivenom, and has important significance for assisting clinical treatment, reducing complications and improving survival rate of patients.

The invention discloses phospholipase A2 inhibitory proteins PLI1 and PLI2 as well as a gene for coding the phospholipase A2 inhibitory proteins PLI1 and PLI2 and application thereof, and belongs to the technical field of gene engineering. In the implementation scheme, the recombinant phospholipase A2 inhibitory protein obtained through a gene shuffling technology is described, the two phospholipase A2 inhibitory proteins PLI1 and PLI2 are screened out through a phage display technology, compared with the inhibitory protein before shuffling, the two phospholipase A2 inhibitory proteins PLI1 and PLI2 still have the good inhibitory effect and activity, and one of the two phospholipase A2 inhibitory proteins PLI1 and PLI2 is obviously superior to the inhibitory protein before shuffling. The invention also comprises polynucleotide for coding the protein and a recombinant vector containing the polynucleotide, and the protein is suitable for preparing the snake venom inhibitor, has broad spectrum and high activity, is expected to realize large-scale mass production, and has wide application prospects.

The present application belongs to the technical field of cosmetic product ingredient identification, and particularly relates to a method for identifying and detecting counterfeit ingredients of counterfeit botulinum toxin cosmetic products. The present application prepares a test sample into a test solution, detects it according to high performance liquid chromatography mass spectrometry, takes telencephalin, acetyl hexapeptide-1 and snake venom-like tripeptide as key indicators for distinguishing counterfeit ingredients of counterfeit botulinum toxin products, and ensures the safety and effectiveness of the botulinum toxin products. The present application only needs to use standard control samples of counterfeit ingredients when the method is established for the first time, and does not need to use control samples in the subsequent routine detection process to accurately determine whether the sample is counterfeit. Moreover, the present application first establishes a method for quantitatively analyzing telencephalin by using liquid chromatography-tandem mass spectrometry. The present application is simple in operation, good in repeatability, high in sensitivity, fast in separation speed, high in detection flux, reliable in results, and effectively ensures the safety and effectiveness of the botulinum toxin products.

The invention discloses a whitening, spot-fading and repairing type anti-aging glass repairing agent with a high-pressure micro-jet nanoscale transdermal absorption function. Comprising the following raw materials in parts by weight: 5-8 parts of vitriol, 3-5 parts of modified shell powder, 1-3 parts of peptides, 1-3 parts of glabridin, 2-3 parts of modified sodium hyaluronate, 2-3 parts of sodium polyglutamate, 4-6 parts of glycerol, 2-3 parts of ceramide NP, 2-3 parts of phosphatidylcholine and 7-10 parts of water. Wherein the peptide substance is prepared from carnosine, snake venom and argireline according to the weight ratio of 1: 1: 1. According to the anti-aging glass repairing agent, ceramide NP and phosphatidylcholine are adopted to be matched with glycerin, meanwhile, the raw materials such as the glass repairing agent, the modified shell powder, the peptides, the glabridin and the modified sodium hyaluronate are matched with the sodium polyglutamate, and the raw materials are blended, coordinated and jointly synergized, so that the obtained product realizes the coordination and optimization of whitening and anti-aging; and the transdermal absorption efficiency of the product is remarkable.

The invention discloses an insect-proof and high-temperature-resistant photovoltaic cable, which is sequentially provided with a wear-resistant layer, an armor layer, an anti-biting layer, a fireproof layer and a wire from the outer layer to the inner layer, and is characterized in that the thickness of the anti-biting layer is at least one sixth of the overall thickness of the photovoltaic cable; the anti-biting layer is prepared from the following components in parts by weight: 30 to 40 parts of phosphorus-silicon resin, 4 to 8 parts of white carbon black, 20 to 30 parts of acrylonitrile-styrene copolymer, 10 to 18 parts of ethylene propylene diene monomer, 5 to 15 parts of polypropylene, 1 to 2 parts of cross-linking agent and 10 to 20 parts of insect-proof medicinal powder; the insect-proof medicine powder is prepared from 5-8 parts of salvia officinalis, 6-10 parts of verbena, 4-8 parts of pepper, 2-4 parts of radix angelicae, 3-6 parts of cynanchum komarovii and 2-4 parts of mint. According to the invention, insect-proof powder in the anti-bite layer has a certain smell and can prevent rats, termites and the like from biting the cable, a certain amount of heat can be generated when the wires are electrified, the heat can be transmitted to the anti-bite layer through the heat conduction columns, the armor layer can absorb a certain amount of solar heat, and the anti-bite layer can absorb the solar energy under the action of the heat on the two sides of the anti-bite layer. The smell emission of the insect-proof medicine powder is facilitated.

The invention belongs to the technical field of biology, and discloses a composition for improving skin inflammation and aging as well as a preparation method and application thereof. The composition is prepared from an exosome, sodium hyaluronate, poly (deoxyribonucleotide) and a snake venom-like peptide. The composition has an unexpected synergistic effect in the aspects of inhibiting expression of IL-6 and reducing the ROS level, the composition is simple in formula, and the composition is matched with corresponding processes and auxiliary materials to be prepared into a required dosage form, so that the problems of skin inflammation and aging can be effectively solved.

The invention relates to a combination preparation for use in a method for the treatment and / or prophylaxis of snakebites, wherein the combination preparation comprises one or more compositions which - Varespladib, methylvarespladib, hydroxy-varespladib, hydroxy-methylvarespladib, methoxy-varespladib, methoxy-methylvarespladib and / or corresponding salts, esters, amides or solvates and - contains a dimercapto chelating agent. The ingredients of the combination preparation complement each other advantageously for the effective treatment or prophylaxis of snakebites while keeping side effects to a minimum.

Provided is an monoclonal antibody for treating envenomation of snake venom of Russell's viper. Also provided is an application of the monoclonal antibodies thereof, including a method for treating Russell's viper envenomation using the monoclonal antibodies.

The invention discloses a composition containing nonapeptide-1, acetyl hexapeptide-8 and arginine lysine as well as a preparation method and application of the composition. The technical problem that an existing product is poor in synergistic interaction is mainly solved. The composition is composed of a core active component, an auxiliary active component and an auxiliary material, the core active component comprises nonapeptide-1, acetyl hexapeptide-8 and a mixture of arginine and lysine in a specific ratio, and the auxiliary active component is selected from snake venom-like peptide or a chrysanthemum extract. Compared with the prior art, by precisely limiting the ratio range of core active ingredients (8-18 parts of nonapeptide-1, 25-35 parts of acetyl hexapeptide-8, 45-55 parts of an arginine-lysine mixture, and the weight ratio of arginine to lysine is 1.5: 1-2.5: 1), the synergistic interaction of three effects of whitening, wrinkle resistance and repairing is creatively realized.

The invention discloses a vector for expressing snake venom thrombin-like bacteria, yeast engineering bacteria and a preparation method, and belongs to the technical field of gene engineering. Based on pPIC9K, pAO815 alpha, thrombin-like snake venom agkihpin and a pichia pastoris expression system, the co-expression copy number of the agkihpin gene and an auxiliary factor is optimized, the copy number of an auxiliary gene Bmh2 is adjusted, and culture parameters such as the initial pH of a culture medium, the methanol addition amount, the induction time, the induction temperature and an auxiliary agent are improved by the system, so that the expression level of the agkihpin is effectively improved, and the expression level of the agkihpin is improved. And a solid foundation is laid for agkihpin industrial application of the thrombin-like snake venom.

The present invention discloses an anti-snake venom drug and its application. The drug comprises apolipoprotein E, or can inhibit the hemorrhagic toxicity, myotoxicity, and coagulative toxicity of snake venom by increasing the concentration or expression of apolipoprotein E, and inhibit the activities of snake venom metalloproteinase (SVMP), snake venom phospholipase A2 (PLA2), and snake venom C-type lectin (CTL). The drug has the potential to be used in the medical field as a new broad-spectrum anti-snake venom drug, improving the problem that existing antivenom serum has high specificity and strong limitations.

The present application relates to the field of cosmetic technology, in particular to a soothing and repairing composition, a skin care product and a preparation method thereof, wherein the composition comprises DNA sodium, a snake venom-like peptide, hydrolyzed silk fibroin, a living particle factor and an ampelopsis grossedentata extract. By controlling the amount of DNA sodium, a snake venom-like peptide, hydrolyzed silk fibroin, a living particle factor and an ampelopsis grossedentata extract in the skin care product, the prepared essence cream, essence liquid and gel have excellent soothing, repairing and anti-wrinkle effects, and meet the use requirements of sensitive skin groups.

The invention discloses a remodeling active peptide and a preparation method thereof, and belongs to the technical field of active peptides. Comprising the following components in parts by weight: 2-4 parts of acetyl octapeptide-3, 2-3 parts of acetyl hexapeptide-8, 2-3 parts of snake venom-like peptide, 3-5 parts of palmitoyl tripeptide-1, 3-5 parts of palmitoyl tetrapeptide-7, 3-5 parts of palmitoyl hexapeptide-12, 1-2 parts of tripeptide-1, 5-10 parts of a traditional Chinese medicine extract composition, 2-3 parts of white honey, 2-3 parts of walnut kernel oil, 3-5 parts of a cubilose extract and 1-2 parts of vitamin E, 2-3 parts of hyaluronic acid, 1-2 parts of ceramide and the balance of rose water. According to the invention, the designed traditional Chinese medicine extract composition can synergistically enhance skin repair, metabolism and circulation functions based on compatibility, forms a comprehensive action network for tonifying middle-Jiao and Qi, activating blood and nourishing skin, harmonizing and removing turbidity and dredging meridians and collaterals, and complements with a molecular signal mechanism of active peptides, so that the skin is improved in a cell layer, a corium layer and a barrier layer.