75 results about "Genetic transfection" patented technology

The invention belongs to the technical field of immunology and in vitro diagnosis, and particularly relates to a rabbit-derived recombinant monoclonal antibody for specific recognition of type II grass carp reovirus VP4 protein, an eukaryotic expression method and application. According to the invention, a target gene S6 is transfected to an HEK293 cell for expression and purification, a New Zealand white rabbit is immunized in cooperation with a Freund's adjuvant, and the rabbit-derived recombinant monoclonal antibody capable of specifically recognizing the GCRV-II VP4 protein is obtained by using ELISA, single B cell screening and eukaryotic expression technologies. The antibody has strong specificity, provides support for further establishment of a specific II-type grass carp reovirus diagnosis technology, and has great application value in development of GCRV-II related scientific research.

The invention discloses a cartilage regeneration and repair method and system based on an irreversible electroporation technology. The method comprises the following steps: establishing an electroporation model of a cell membrane, simulating the distribution of an electric field and the potential change of the cell membrane, and predicting a membrane perforation effect; analyzing potential change according to an electroporation model, and combining energy consumption change of the cell membrane to obtain a critical condition of cell membrane pore formation; in combination with critical conditions, establishing an adaptive adjustment feedback mechanism, controlling the adjustment of electroporation parameters, and performing cartilage related gene transfection and cartilage repair; setting a comprehensive evaluation function, evaluating the repairing effect of the cartilage tissue, and adjusting electroporation parameters and a gene transfection strategy. The system comprises an electroporation model modeling module, a critical analysis module, an adaptive mechanism establishment module, a transfection repair module and an evaluation adjustment module. According to the method, the transfection efficiency of cartilage repair related genes can be improved and cartilage regeneration can be promoted by accurately regulating and controlling electroporation parameters and combining a self-adaptive feedback mechanism.