35 results about "Embryo transfer" patented technology

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The utility model provides a kind of embryo transfer device with physical locking function belongs to biological sample experimental equipment field.The device includes isolation cabinet, storage box and transmission disc, and storage box and transmission disc are disc-shaped structure, and storage box includes bottom plate and top cover, and transmission disc is rotatably arranged between bottom plate and top cover, and transmission disc is provided with multiple placing cavities along circumference, and top cover is provided with first placement port and second placement port corresponding with placing cavity, and first placement port and second placement port are respectively located at both sides of isolation cabinet, and when first placement port corresponds with one of placing cavities, second placement port is staggered with other placing cavities. The embryo transfer device with physical locking function provided by the utility model embodiment can solve the problem of high risk of medical accidents in the prior art.

The invention belongs to the technical field of medical instruments, and provides an embryo transfer catheter auxiliary device which comprises an inner supporting core and a heating wire. The inner supporting core is formed by coaxially connecting a supporting rod and a flexible pipe with a closed front end, the flexible pipe is filled with a phase change material, and the heating wire penetrates through the flexible pipe and is used for heating and softening the material. A containing cavity is formed in the supporting rod, a slidable piston is arranged in the containing cavity, the multiple supporting pieces are connected in series through a connecting rope and connected to the piston, and the farthest end abuts against the closed end of the flexible pipe. The piston moves to drive the supporting piece to enter and exit from the flexible pipe, and dynamic adjustment of the volume of the phase-change material is achieved. The supporting piece is made of an ultrasonic visual material or structure, and intraoperative positioning is facilitated. Due to the adoption of the structure, the device provides rigid support during catheter placement, can actively reduce the volume and soften after positioning, realizes non-traction and low-resistance withdrawal, supports ultrasonic real-time guidance, and remarkably improves the positioning precision and the operation safety of the outer tube.

The invention discloses a leakproof efficient embryo flushing pipe for horse embryo transplantation, and relates to the technical field of horse breeding instruments.The leakproof efficient embryo flushing pipe comprises a main pipe, the bottom end of the main pipe is communicated with an embryo flushing liquid input pipe and an embryo flushing liquid output pipe, the embryo flushing liquid input pipe is communicated with a pump-out unit, the embryo flushing liquid output pipe is communicated with a recovery unit, and the upper portion of the main pipe is sleeved with an outer pipe; the top end of the main pipe is communicated with two blank flushing pipes respectively; the two blank flushing pipes are folded to form a cylindrical structure and have the same diameter as the outer pipe; driving combining assemblies are arranged on the opposite sides of the two blank flushing pipes and used for driving the two blank flushing pipes to be combined or separated; an air bag plugging assembly and a rubber plugging assembly are sequentially arranged at the end, close to the blank flushing pipe, of the outer pipe from top to bottom. After the two embryo flushing pipes extend into the uterus, the two embryo flushing pipes are separated through the driving combining and separating assembly and face the uterus horns on the two sides respectively, and the flushing and recycling efficiency is greatly improved; the cervix is blocked through the air bag blocking assembly and the rubber blocking assembly, safety and reliability are achieved, and flushing fluid is prevented from leaking out.

The utility model aims to provide a device for safely transferring an embryo transfer tube, which relates to the technical field of medical instruments and is characterized in that an embryo transfer device is designed, so that a bracket main body can be accommodated in a constant-temperature box, and a fixing device on the bracket main body can fix the embryo transfer tube; one end of the fixing device is higher than the other end of the fixing device, so that the orifice of the fixed embryo transfer tube is inclined upwards, and the embryo is effectively prevented from sliding out of the transfer tube; by means of the triangular supporting structure of the handheld part, medical staff can stably transfer an embryo transfer tube to a patient for an operation, shaking of the embryo transfer tube in the transfer process is effectively avoided, in the transfer process, the constant-temperature box can meet the environment condition for embryo storage, the patient can confirm the embryo through an observation opening, a nurse does not need to take out the transfer tube, and operation is convenient. And sliding-out in the taking-out process is avoided, and the problems that the transfer efficiency is low, and transfer operation is not convenient enough are effectively solved.

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention discloses a construction method of a Dcaf17 gene modified golden hamster model and application of the Dcaf17 gene modified golden hamster model. The method comprises the following steps: under a red light source, injecting a Cas9 functional element and sgRNA of a targeted Dcaf17 gene exon 1 through a two-cell embryo, and transplanting the embryo into a receptor body, so as to obtain the Dcaf17 gene modified golden hamster. The Dcaf17 gene modified golden hamster model is constructed by utilizing two-cell stage embryo gene editing, and the Dcaf17 gene modified golden hamster homozygous male rat has the phenotype characteristics of no fertilization ability, normal testis and the like, and is used for replacing ligation male rats to prepare false pregnancy receptor rats. The receptor can efficiently identify and utilize a false pregnancy receptor through microscopic examination of sperms by a microscope. Therefore, the invention solves the technical difficulty of receptor preparation in related experiments of assisted reproduction of the golden hamster, and can be used for phenotype research of the Dcaf17 gene and related applications of assisted reproduction of the golden hamster.

The utility model relates to a device for improving embryo culture dish transfer efficiency and safety, which comprises an incubator arranged on a table top in an IVF (in-vitro fertilization) workstation and a box cover arranged on the incubator and capable of opening and closing the incubator, the incubator is arranged on the right side of a stereoscopic microscope fixing column and is positioned in front of the right of a heating plate below an objective lens of a stereoscopic microscope, and the box cover is arranged on the right side of the stereoscopic microscope fixing column. The bottom of the IVF work station is provided with a pedal auxiliary switch used for controlling opening and closing of the box cover. The box cover comprises an overweight box cover body, a lever fixedly connected with the overweight box cover body, a linkage rod rotationally connected to the tail of the lever, a vertical rod rotationally connected to the tail of the linkage rod and capable of vertically moving, a cam block used for driving the vertical rod to move up and down, a mounting shell used for mounting the cam block and a servo motor used for driving the cam block. The incubator arranged on the table board is arranged in the table board of the IVF work station, and a pedal switch is treaded by feet to control the box cover, so that two hands are liberated, and the speed and the safety of transferring a culture dish between the incubator and the stereoscope heating table are improved.

The utility model relates to the technical field of bottle production lines, in particular to a rotary bottle blowing machine. The rotary bottle blowing machine is provided with a guide assembly, the guide assembly comprises a first guide plate and a second guide plate, the first guide plate is installed above the protective plate, the second guide plate is located above the transfer gear, the first guide plate and the second guide plate are arranged in a spaced mode to form a guide gap, and the part, above the supporting ring, of the bottle preform can be clamped in the guide gap. The upper portion of the bottle preform supporting ring is fixed in the guide gap, so that the single fixing mode of the bottle preform on the lower side of the supporting ring is changed into the double fixing mode of the upper side and the lower side of the supporting ring, the stability of the bottle preform in the transferring process is improved, and the bottle preform is prevented from shaking left and right; and the axial line of the bottle blank and the extending direction of the heating head are parallel to the rotating axial direction of the transfer assembly, so that the accuracy and success rate of inserting the heating head into the bottle blank are improved, and the bottle blank is prevented from being damaged.

The utility model discloses a kind of hair-planting and hair-trimming combination machines, including brush embryo conveying mechanism, manipulator, brush embryo transfer mechanism, hair-planting machine and hair-trimming machine, the brush embryo transfer mechanism carries out horizontal transport to brush embryo, the hair-planting machine and hair-trimming machine are located at the same side of brush embryo transfer mechanism moving route, the brush embryo conveying mechanism and manipulator are located at the side of brush embryo transfer mechanism back to hair-trimming machine, the manipulator is gripped and conveyed to brush embryo transfer mechanism by brush embryo conveying mechanism on brush embryo, the brush embryo transfer mechanism is horizontally conveyed to hair-planting machine and hair-trimming machine, brush embryo conveying mechanism is conveyed to conveying end then under the action of manipulator is transferred to brush embryo transfer mechanism, brush embryo transfer mechanism horizontal conveying realizes the automatic conveying of brush embryo between hair-planting machine and hair-trimming machine, realizes the good automatic conveying of brush embryo, effectively reduces artificial, and improve production efficiency, good realization brush embryo's automatic hair-planting and automatic hair-trimming.

Disclosed in the present invention are a composition and method for increasing the number and proportion of inner cell mass cells of an in vitro embryo and use. According to the present invention, in view of the problems of in vitro fertilized embryos such as low blastocyst rate during early development, low inner cell mass cell number in the blastocyst stage, low proportion of the inner cell mass cells, and poor subsequent embryonic development potential, by adding one or two of regulators folic acid and laxiflorin B into the culture medium for early embryo in vitro culturing, the blastocyst rate, the number and proportion of the inner cell mass cells in the blastocyst, the survival rate of blastocysts after extended culturing, and the diameter of blastocysts are significantly improved, effectively improving the developmental potential of in vitro embryos. In addition, the present invention also effectively improves the ratio of in vitro fertilized embryos that can be subsequently transplanted. The present invention is low in cost, safe, and efficient, provides a new strategy for in vitro embryo production of livestock and development of human-assisted reproductive technology, and exhibits broad application prospects.

The invention includes a method of identifying high genomic value, non-human mammalian embryos that would otherwise be disposed of or frozen based on their morphology. The method generally involves establishing a stem cell culture from the non-human mammalian embryo, which entails generating a population of embryos in vitro or in vivo, assessing the morphology of each of the embryos in the population, selecting a subpopulation of embryos for embryo transfer based on the morphological assessment, thereby establishing a group of selected embryos and a group of unselected embryos in the population, and establishing a stem cell culture from an embryo in the group of unselected embryos.

Methods and formulations for a simplified, single-injection method to induce and control the synchronous growth (superstimulation), and ovulation (superovulation) of multiple ovarian follicles in bovine, ovine, caprine, camelid and other female animals enabling the subsequent collection of (a) multiple oocytes when conducting in-vitro fertilization, or (b) multiple embryos when conducting multiple ovulation embryo transfer.

This invention discloses a method for constructing and applying an anti-obesity animal model based on ISCA1 / 2 gene regulation, belonging to the field of biomedical technology. The method utilizes CRISPR-Cas9 gene editing technology to design specific single-stranded guide RNAs targeting the ISCA1 and ISCA2 genes in mice. Cas9 mRNA and sgRNA are introduced into fertilized eggs of C57BL / 6J strain mice via microinjection. The injected embryos are then transferred into pseudopregnant mice to obtain F0 generation chimeric mice. By mating F0 generation mice with wild-type C57BL / 6J mice, F1 generation heterozygous mice carrying the target mutation in the germline are selected, thus obtaining the anti-obesity animal model. This model is not only a phenotypic model but also a mechanistic research model, suitable for drug target validation, energy metabolism pathway exploration, and personalized treatment strategy evaluation.

The invention discloses a method for rapidly constructing a Foxn1 homozygous nude mouse through RepSox induction based on CRISPR / Cas9, and the method comprises the following steps: synthesizing two sgRNAs for respectively identifying a 5'terminal target site and a 3 'terminal target site, the nucleotide sequence of the sgRNA for identifying the 5' terminal target site is as shown in SEQ ID NO: 1, and the nucleotide sequence of the sgRNA for identifying the 3 'terminal target site is as shown in SEQ ID NO: 2; a mixed solution of the two sgRNAs, the Cas protein and the RepSox is microinjected into the fertilized eggs of the rats; the injected embryos are subjected to transient in-vitro culture, survival embryos are screened and transplanted into fallopian tubes or uteruses of pseudopregnant female rats for development, and F0-generation Foxn1 knockout homozygous rats are obtained. According to the method, the Foxn1 gene can be accurately knocked out, the survival rate of a gene editing embryo is improved, the model construction period is shortened, and the rat knockout efficiency is remarkably improved.

The invention discloses a library building method for detecting G-quadruplex structures in oocytes, early embryos and trace cells, and belongs to the technical field of whole genome immunoprecipitation library building. The DNA G-quadruplex structure provided by the invention is a DNA secondary structure which is different from a classic double-helix structure; g-quadruplex structure abnormity can damage telomere stability, genome stability, transcriptional activity and the like; this is an important reason for poor ovum quality and embryonic development retardation. The method provided by the invention can be used for evaluating the quality of the ova of the infertile women and judging reasons causing immaturity of the ova; abnormal enrichment of in-vivo and in-vitro development arrest embryo whole genome G-quadruplex is detected and can be used as an important index for screening reasons causing embryonic development retardation; the method can be used for guiding whether corresponding clinical patients are suitable for a series of assisted reproductive technologies (ART) such as in vitro fertilization-embryo transfer (IVF-ET) and the like.

This invention relates to a visual embryo transfer catheter, comprising a catheter portion including a catheter, a support tube, a first seat, a sensing assembly, and a display screen. The support tube is disposed on one side of the catheter. The first seat is disposed at one end of the support tube. The catheter lumen communicates with a cavity on the first seat. An annular cavity is formed on the wall of the catheter. The sensing portion of the sensing assembly extends into the annular cavity and can monitor at least a section of the catheter lumen near its free end. The sensing assembly is connected to the display screen to display the monitored condition within the catheter lumen. This invention enables rapid confirmation during embryo transfer of whether the embryo has been adequately pushed into the uterus, thus helping to shorten the time the catheter remains in the uterus and reducing uterine stimulation during embryo transfer.

The utility model discloses a kind of brush embryo transfer mechanism, including main body, the bottom of the main body has the sliding block for horizontal sliding connection, swing arm is rotatably connected on the main body, the end of the swing arm is provided with clamp for clamping brush embryo, the first drive source for driving swing arm rotation is arranged on the main body, clamp on main body can be used to clamp brush embryo, then by the second drive source driving main body horizontal movement realizes the transport of brush embryo, after clamping brush embryo, it can be horizontally transported to the place of hair-planting machine and hair trimming machine, by the first drive source, swing arm can be driven to rotate to realize the steering of before and after, facilitate the grasping and placement of brush embryo, realize the good automatic transport of brush embryo, effectively reduce artificial, improve production efficiency.

A method of erasing an abnormal epigenetic modification in a sheep iCHI embryo and use thereof. A method including the steps of: transferring a recombinant plasmid expressing protamine into a sheep androgenetic haploid embryonic stem cell, then introducing the cell into a mature sheep oocyte, and performing activation and culture to obtain a sheep iCHI embryo without abnormal epigenetic modifications. In the present disclosure, abnormal methylations in iCHI embryos may be erased by transient expression of protamine in o-haSCs. The blastocyst rate of the prepared Pro-iCHI embryos is significantly higher than that of iCHI embryos, and is similar to that of IVF embryos. The resulting embryos can be used for embryo transfer to obtain semi-cloned gene-edited sheep, providing materials for subsequent breeding applications.

The copy number of the ten-gene edited porcine endogenous retroviruses (PERVs) in a whole genome is determined by ddPCR, and the PERV-Pol genotype is determined through second-generation whole genome deep sequencing. The method comprises the following steps: constructing a porcine PERV-Pol gene knockout targeting vector, co-transfecting the vector into a ten-gene edited porcine fetal fibroblast line, screening to obtain a PERV-Pol knockout fibroblast line, carrying out somatic cell nuclear transfer and embryo transfer, taking out a fetus during pregnancy, and identifying PERV-Pol gene knockout and PERVs inactivation conditions of the fetus. Furthermore, the continuous cloning technology is used for batch production of the PERVs completely inactivated eleven-gene edited xenotransplantation donor pigs. The technical problems that the PERV-Pol gene, especially the multi-copy gene, is high in editing difficulty, low in efficiency and long in period are solved, and the method has important significance on overcoming the problems of biosafety and immunological rejection in the xenogeneic organ transplantation process.

The invention relates to the technical field of biology, in particular to a method for constructing a gene editing animal model and application of the gene editing animal model, and the method comprises the following steps: S1, obtaining an oocyte or an embryo before implantation of an animal; s2, preparing a delivery compound, and enabling the oocyte or the pre-implantation embryo to be in contact with the delivery compound, so as to obtain the oocyte or the pre-implantation embryo subjected to gene editing treatment; and S3, transferring the pre-implantation embryo subjected to gene editing treatment into a uterus suitable for being used as an embryo transfer receptor animal to obtain a gene editing animal model. According to the method for constructing the gene editing animal model, a non-invasive delivery mechanism is adopted, and the gene editing efficiency as high as 100% can be achieved through a delivery compound; the method has the advantages of being high in construction success rate, capable of accurately achieving single-gene or multi-gene knockout, high in embryo survival rate, easy to operate, low in cost, short in period, suitable for construction of various animal models and the like, and the application prospect is wide.

The invention belongs to the technical field of animal breeding. More specifically, the invention relates to a method for predicting ewe embryo transplantation pregnancy results. The research finds that luteinizing hormone, cortisol and prostaglandin E2 in the estrus period of the ewe have significant correlation with the pregnancy result after the ewe embryo transplantation in a specific concentration range for the first time, and ROC curve analysis proves that the three can be used as biomarkers for predicting the pregnancy result after the ewe embryo transplantation. On the basis, the invention provides a method for predicting the pregnancy result of ewe embryo transplantation, and the pregnancy result is predicted according to the concentration of the reproductive hormone by detecting the concentration of the reproductive hormone in the peripheral blood of the receptor ewe in the estrus period. According to the method, pregnancy potential evaluation can be realized before transplantation, and the prediction timeliness is remarkably improved. In addition, breeding managers can screen high-pregnancy-potential individuals accordingly, and breeding efficiency is improved. The hormone abuse condition in embryo transplantation is reduced, the culture cost is reduced, and the method has good application prospects and value.

The invention discloses a handheld embryo transfer operation device which comprises a silica gel suction head, the silica gel suction head is communicated with one end of a middle connecting pipe, the other end of the middle connecting pipe is communicated with one end of a filter, and the other end of the filter is communicated with a connecting hard pipe; the embryo transfer device is easy and convenient to operate and low in cost, the air pressure buffer area is prolonged through the filter and the middle connecting pipe, the swing amplitude of the suction end is reduced, the suction force to an embryo and the amount of brought-in liquid are accurately controlled, cross contamination is avoided, and meanwhile embryo transfer operation is more stable.

The invention discloses an sgRNA combination for efficiently knocking out a sheep MSTN gene and application, and belongs to the field of genetic engineering and genetic breeding of livestock. The sgRNA combination comprises sg-M2 and sg-M3, the nucleotide sequence of the sg-M2 is as shown in SEQ ID NO: 1, and the nucleotide sequence of the sg-M3 is as shown in SEQ ID NO: 2. By utilizing the sgRNA combination of the sg-M2 and the sg-M3 provided by the invention, the sgRNA combination and Cas9 mRNA / protein are microinjected into a sheep embryo, and an offspring is obtained through embryo transplantation. Through genotype identification of offspring sheep, the MSTN gene editing efficiency reaches 100%, and the functional structure domain knockout efficiency can reach 80.0%. The preparation cost of the MSTN gene knockout sheep can be effectively reduced, and an effective means is provided for rapidly creating new germplasm of the double-muscle gluteal mutton sheep.

The copy number of the eight-gene edited PERVs in a genome is measured through ddPCR, and the PERV-Pol genotype is determined by utilizing second-generation whole genome deep sequencing. The method comprises the following steps: constructing a porcine PERV-Pol gene knockout targeting vector, jointly transfecting the vector into an eight-gene edited porcine fetal fibroblast line, screening to obtain a PERV-Pol knockout positive fibroblast line, carrying out somatic cell nuclear transfer and embryo transfer, taking out a fetus after pregnancy, and identifying PERV-Pol gene knockout and PERVs inactivation conditions of the fetus. And designing and constructing a targeting sgRNA expression vector again according to the knockout condition, and transfecting the targeting sgRNA expression vector into the PERV-Pol gene knockout fetal fibroblast line until the nine-gene edited pig fibroblast line with completely inactivated PERVs is obtained through screening for somatic cell nuclear transplantation, thereby finally obtaining the nine-gene edited xenotransplantation donor pig with completely inactivated PERVs. According to the invention, the technical problems of high difficulty and low efficiency of editing the endogenous retrovirus, especially the multi-copy gene, are solved, and the problems of biological safety and immunological rejection in the xenogenic organ transplantation process are solved.

The utility model relates to a kind of combined non-surgical method super-deep embryo transfer insemination tube for pig, and the transfer tube includes: ordinary insemination tube, super-deep insemination tube and super-deep embryo transfer tube, which is used for the transport of embryo or semen after combination. The ordinary insemination tube is used for inserting into vagina dilatation cervix, so that the super-deep insemination tube can pass through vagina and cervix. The super-deep insemination tube has a metal connector and a silicone soft tip at its front end. The super-deep embryo transfer tube has a standard luer taper syringe interface at its tail end. Compared with the prior art, the utility model is specially developed according to the existing problems of non-surgical embryo transfer, and is innovative on the basis of retaining the advantages of non-surgical embryo transfer. Under the visual field, the catheter can be accurately inserted into the specified position, which can prevent embryo reflux and effectively solve the problems of blind insertion method, such as uncontrollable insertion depth, bending of embryo transfer tube and embryo reflux, thereby improving the efficiency of non-surgical embryo transfer pregnancy delivery.

This utility model discloses a nutritional support delivery device after embryo transfer, belonging to the technical field of embryo transfer equipment. It includes a timed and quantitative supply mechanism, which delivers internal nutrients at specific time intervals and volumes. This utility model achieves convenient disassembly and precise flow control in one integrated system. Its modular design utilizes a positioning groove on a fixed plate that engages with a built-in spring-loaded round-headed block. During installation, the positioning block of the outer fixed platform of the supply mechanism is pushed into the groove, and the positioning hole aligns with the round-headed block, triggering the spring self-locking to ensure stability and prevent displacement. This also facilitates disassembly, replacement, and cleaning, improving safety. The core flow control relies on a drive motor to rotate a perforated disc. The disc's through-holes periodically align with or offset the leakage holes at the bottom of the storage cylinder, achieving on / off control of the liquid flow. By adjusting the motor speed, the supply interval and single-volume output are precisely adjusted, achieving reliable timed and quantitative release.

This utility model relates to the field of assisted reproductive technology, specifically to an embryo transfer tube, comprising a first tube segment and a second tube segment connected to each other. The inner diameter of the second tube segment is larger than that of the first tube segment. A piece of absorbent cotton is placed inside the second tube segment. An annular limiting seat is provided on the inner wall of the second tube segment at the end away from the first tube segment, and the inner diameter of the annular limiting seat is smaller than that of the second tube segment. By setting an annular limiting seat at the end of the larger-diameter second tube segment, this utility model can reduce the air intake cross-sectional area, increase airflow resistance, and make the negative pressure generated by the suction bulb more gradual. This facilitates the operator's precise control of the embryo aspiration position, thereby reducing the risk of embryos being accidentally aspirated into the second tube segment. Simultaneously, the absorbent cotton placed inside the second tube segment further intercepts any embryos and fluids that may be accidentally aspirated.