94 results about "Embryo transfer" patented technology

The embryo transfer device comprises a clamp, a carrying rod and a sleeve, the clamp is provided with a structure used for installing the carrying rod and the sleeve, the carrying rod and the sleeve are matched with the structure in an embedded mode, the carrying rod and / or the sleeve and components on the structure generate acceleration through relative acting force, and the clamping device is used for clamping the carrying rod and / or the sleeve and the components on the structure. And the embryo on the carrying rod falls into the sleeve. The embryo transfer can be completed without a microscope and a mouth suction tube, and the embryo transfer device is convenient to use in pastures and remote environments. According to the embryo transfer device, embryo transfer can be completed without a microscope and a mouth suction tube, the transferred embryo is almost free of damage, the situation that the embryo is lost in the transfer process is avoided to the maximum extent, and the embryo transfer device further has the advantages of being simple in structure, easy to implement, easy to operate, low in cost and the like.

The present application relates to the technical field of warming machine, disclose a kind of embryo inserting device and warming machine.Warming machine includes embryo inserting device and rack, embryo inserting device includes rotating mechanism and several bottom supporting mechanisms, rotating mechanism can be rotated relative to rack around vertical axis, rotating mechanism is provided with several first matching parts along the circumference, and the first matching part is used to limit the upper end of bottle embryo;Several bottom supporting mechanisms are connected with rotating mechanism and are correspondingly arranged below several first matching parts, and bottom supporting mechanism can move upward along vertical direction at preset time, to lift and guide the lower end of bottle embryo located at corresponding first matching part.The warming machine of the present application can ensure that the bottle embryo clamping mechanism is accurately inserted and grasped by setting the above-mentioned embryo inserting device, and improve the yield of bottle embryo processing.

The construction of a non-human primate Usher syndrome disease model having USH2A gene mutation, wherein a target sequence against the mRNA of the non-human primate USH2A gene is established and a corresponding sgRNA is synthesized, then a knockout vector is constructed, the sgRNA and a Cas9 mRNA are mixed and injected into a non-human primate embryo to obtain a non-human primate embryo having USH2A gene knockout, and then by means of embryo transfer, a non-human primate having USH2A gene knockout is obtained, which exhibits symptoms of Usher syndrome. Upon detection and identification of the knockout effect, the knockout of the USH2A gene can be effectively realized in a non-human primate cynomolgus monkey embryo and cynomolgus monkeys, which provides a new and effective disease model for the diagnosis and treatment of Usher syndrome in non-human primates.

The invention belongs to the technical field of bottle blowing machines, and discloses a blank feeding device of a bottle blowing machine and the bottle blowing machine. The blank feeding device comprises a left blank feeding seat, a right blank feeding seat, a blank feeding star wheel, a blank blocking mechanism and a telescopic mechanism, the left blank feeding seat and the right blank feeding seat are arranged at an interval and define a blank feeding channel, one of the left blank feeding seat and the right blank feeding seat is provided with a bottle blowing air nozzle, the blank feeding channel is provided with a notch, the blank feeding star wheel is provided with a plurality of wheel teeth, and part of the wheel teeth are located in the blank feeding channel; when the blank feeding star wheel rotates, bottle blanks in the blank feeding channel can be pushed to move, the blank blocking mechanism is provided with a blank blocking piece capable of stretching into the blank feeding channel from the notch so as to block the bottle blanks from moving, and the telescopic mechanism is provided with a movable piece corresponding to the notch. According to the blank feeding device, when the tooth tips of the blank feeding star wheel abut against the bottle blanks, the bottle blanks can be discharged out of the blank feeding channel in time, the phenomenon of blank feeding overload is avoided, and the sudden stop probability of equipment is reduced.

A visual embryo transfer catheter system includes a handle and a visualization sheath which is configured to be coupled to the handle and to be inserted into the uterus of a patient. The visualization sheath includes at least three lumens, including a first lumen for a scope assembly including a camera and an illumination source, a second lumen to permit passage of an embryo transfer catheter which is configured to deliver an embryo to the uterus upon imaging of the uterus with the camera when the uterus is illuminated by the illumination source, and a third fluid supply lumen for inflow and outflow of fluid to and from the uterus. Further embodiments include methods for transferring an embryo into a uterus of a patient under visual guidance, and kits for performing a visualized embryo transfer.

The embryo transfer device comprises a clamp, structures and mechanisms, the structures are arranged on the clamp and used for containing embryos, the mechanisms are used for enabling the embryos to fall off from the structures, and the mechanisms apply acceleration to the structures to enable the embryos to fall off from the structures into containers. The invention also comprises an embryo transfer method which comprises the following steps: placing an embryo on a structure; placing the structure in a container; acceleration is applied to the structure manually or through a mechanism, so that the cells fall off from the structure into the container. According to the invention, cells on the structure are transferred into the container by shifting the structure, embryo transfer can be completed without a microscope and a mouth suction tube, and the device is convenient to use in pastures and remote environments. The transferred embryos are almost free of damage, the situation that the embryos are lost in the transferring process is avoided to the maximum extent, and the embryo transferring device further has the advantages of being simple in structure, easy to implement, easy to operate, low in cost and the like.

The present disclosure relates to devices and methods for accessing the reproductive tract of an animal, particularly via the animal's vagina, and deploying reproductive material within the uterus. The device and methods of the disclosure may be particularly suited for embryo transfer processes, such as performed in livestock e.g., cattle.

The utility model discloses a multi-cavity continuous heating bottle blowing machine pitch changing mechanism which comprises a plurality of blank feeding guide rail cushion blocks, a blank moving X air cylinder seat is installed on the tops of the blank feeding guide rail cushion blocks, a first air cylinder is installed on the blank moving X air cylinder seat, a first linear sliding block guide rail is installed on the tops of the blank feeding guide rail cushion blocks, and a second linear sliding block guide rail is installed on the linear sliding block guide rail. A blank moving X driving seat is mounted on an upper slide way of the linear slide block guide rail I, and the output end of the air cylinder I is connected with the blank moving X driving seat; a blank moving Y-shaped material is installed on the top of the blank moving X-shaped driving base, a blank moving Y-shaped air cylinder base is installed on the blank moving Y-shaped material, a front blank moving block and a rear blank moving block are installed on the blank moving Y-shaped material in a sliding way, and the front blank moving block and the rear blank moving block are installed on the blank moving Y-shaped material through a blank moving Y-shaped driving base sliding way. The stroke of the second air cylinder and the stroke of the third air cylinder are different, two bottle blanks are pulled apart by a certain distance (bottle blowing distance) from the initial adjacent distance through the second air cylinder and the third air cylinder, the distance changing function is achieved, the structure is simple, and operation is easy.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The utility model provides a kind of embryo transfer device with physical locking function belongs to biological sample experimental equipment field.The device includes isolation cabinet, storage box and transmission disc, and storage box and transmission disc are disc-shaped structure, and storage box includes bottom plate and top cover, and transmission disc is rotatably arranged between bottom plate and top cover, and transmission disc is provided with multiple placing cavities along circumference, and top cover is provided with first placement port and second placement port corresponding with placing cavity, and first placement port and second placement port are respectively located at both sides of isolation cabinet, and when first placement port corresponds with one of placing cavities, second placement port is staggered with other placing cavities. The embryo transfer device with physical locking function provided by the utility model embodiment can solve the problem of high risk of medical accidents in the prior art.

The purpose of the present invention is to provide an excellent infertility treatment capable of improving the implantation rate of an embryo (fertilized egg) by improving the uterus environment. The present invention is a composition for treating uterus, which contains a mesenchymal stem cell culture supernatant, and which is used as a uterus environment-improving agent, an implantation aid, an embryo transplantation solution, a sperm transplantation solution or an implantation rate-improving agent. The mesenchymal stem cells are preferably derived from adipose tissue, umbilical cord tissue or bone marrow tissue.

The utility model discloses a kind of mechanical hand centering equipment of curing press, belong to tire curing press auxiliary component field, a kind of mechanical hand centering equipment of curing press, including curing press center machine, the upper end of the curing press center machine is fixedly connected with base, the inner wall between the base is equipped with carousel, the upper end of the base is fixedly connected with limit frame, four centering plates are movably connected between the limit frame and carousel, four The centering plate is distributed in cross shape, and laser ranging probe is embedded and installed in outer end, can be transferred to the embryo when curing press center machine is above, the embryo that can be transferred is automatically centered, and offset error value data can also be transmitted to the operating program of mechanical hand, so that mechanical hand secondary embryo transfer, position adjustment can be made in time, reduce subsequent personnel repeatedly loaded embryo position commissioning step, speed up work efficiency.

To provide a composition and a method for administering an oxytocin antagonist to a subject undergoing embryo implantation therapy so as to improve endometrial receptivity and reduce the likelihood of embryo implantation failure in the subject.SOLUTION: The present invention provides a pharmaceutical composition for use in a method that employs a compound represented by formula (II) to treat a subject undergoing embryo implantation therapy, in which one or more embryos are transplanted into the uterus of the subject, wherein the method comprises administering the compound to the subject in an amount of about 1,800 mg.SELECTED DRAWING: Figure 1

This embryo transfer device kit comprises: a connection component (2) which is connected to the base end of a tube the leading end thereof being disposed inside a uterus and the base end thereof disposed outside the uterus; and an embryo transfer device (1), at least a portion of which is housed in a housing unit (71A) that is provided to the connection component (2) so as to extend in a prescribed direction. The embryo transfer device (1) is provided with an embryo storage unit (20) having a recess (21) that opens in a direction intersecting with the prescribed direction. In a state in which the embryo storage unit (20) is disposed in a uterine cavity, the embryo storage unit holds an embryo, which is disposed in the recess (21), in a space surrounded by the recess (21) and the endometrium of the uterine cavity which is present in the opening direction of the recess (21).

The present invention relates to the field of animal husbandry and veterinary technology, and specifically to a combined preparation for improving the conception rate of bovine embryo transplantation, and a preparation method and application thereof. The combined preparation comprises preparation A and preparation B; the active ingredients of preparation A are sodium selenite and vitamin E; the active ingredients of preparation B are chorionic gonadotropin and luteinizing hormone releasing hormone A3. By intramuscularly injecting preparation A with active ingredients of sodium selenite and vitamin E into the recipient bovine for embryo transplantation at 8 and 11 months of age, and intramuscularly injecting preparation B with active ingredients of chorionic gonadotropin and luteinizing hormone releasing hormone A3 into the recipient bovine for embryo transplantation on the day of embryo transplantation, the conception rate of bovine embryo transplantation can be improved. When preparation A and preparation B are used together at the same time, the transplantation conception rate can be significantly improved.

The invention discloses a novel embryo transplant gun for cattle which can bend and extend forward along with the uterine horn, and relates to the field of embryo transplant guns. The invention comprises a gun barrel, a gun core and a culture fluid capillary, wherein the culture fluid capillary is matched and installed in a barrel hole at the inner end of the gun barrel, a piston is arranged in the culture fluid capillary, the gun core is arranged in the gun barrel, and the gun barrel also comprises an inflatable part, a hollow steel needle and an extension catheter, wherein the extension catheter is a hose with a hollow interior. The invention pushes the embryo in the capillary into the extension catheter through the piston, and then pierces the piston through the hollow steel needle to communicate with the inner cavity of the capillary, an operator pushes the piston handle of the syringe to allow the gas in the syringe to enter the extension catheter through the hollow steel needle, and relies on the gas to inject the embryo in the extension catheter into the uterine horn of the cattle, thereby solving the problem that the gun core cannot inject the embryo into the uterus after the capillary is connected to the extension catheter, and the operator does not need to grab the front end of the uterine horn, thereby greatly reducing the operating difficulty, facilitating the use of the operator, and indirectly improving the conception rate.

The invention belongs to the technical field of medical instruments, and provides an embryo transfer catheter auxiliary device which comprises an inner supporting core and a heating wire. The inner supporting core is formed by coaxially connecting a supporting rod and a flexible pipe with a closed front end, the flexible pipe is filled with a phase change material, and the heating wire penetrates through the flexible pipe and is used for heating and softening the material. A containing cavity is formed in the supporting rod, a slidable piston is arranged in the containing cavity, the multiple supporting pieces are connected in series through a connecting rope and connected to the piston, and the farthest end abuts against the closed end of the flexible pipe. The piston moves to drive the supporting piece to enter and exit from the flexible pipe, and dynamic adjustment of the volume of the phase-change material is achieved. The supporting piece is made of an ultrasonic visual material or structure, and intraoperative positioning is facilitated. Due to the adoption of the structure, the device provides rigid support during catheter placement, can actively reduce the volume and soften after positioning, realizes non-traction and low-resistance withdrawal, supports ultrasonic real-time guidance, and remarkably improves the positioning precision and the operation safety of the outer tube.

The present invention relates to dispensing instruments and methods for introducing treatment material and fluid-like material with an embryo into a uterus. More specifically, the present invention relates to apparatuses for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species and associated computer programs for controlling said apparatuses.

The invention discloses a leakproof efficient embryo flushing pipe for horse embryo transplantation, and relates to the technical field of horse breeding instruments.The leakproof efficient embryo flushing pipe comprises a main pipe, the bottom end of the main pipe is communicated with an embryo flushing liquid input pipe and an embryo flushing liquid output pipe, the embryo flushing liquid input pipe is communicated with a pump-out unit, the embryo flushing liquid output pipe is communicated with a recovery unit, and the upper portion of the main pipe is sleeved with an outer pipe; the top end of the main pipe is communicated with two blank flushing pipes respectively; the two blank flushing pipes are folded to form a cylindrical structure and have the same diameter as the outer pipe; driving combining assemblies are arranged on the opposite sides of the two blank flushing pipes and used for driving the two blank flushing pipes to be combined or separated; an air bag plugging assembly and a rubber plugging assembly are sequentially arranged at the end, close to the blank flushing pipe, of the outer pipe from top to bottom. After the two embryo flushing pipes extend into the uterus, the two embryo flushing pipes are separated through the driving combining and separating assembly and face the uterus horns on the two sides respectively, and the flushing and recycling efficiency is greatly improved; the cervix is blocked through the air bag blocking assembly and the rubber blocking assembly, safety and reliability are achieved, and flushing fluid is prevented from leaking out.

The utility model aims to provide a device for safely transferring an embryo transfer tube, which relates to the technical field of medical instruments and is characterized in that an embryo transfer device is designed, so that a bracket main body can be accommodated in a constant-temperature box, and a fixing device on the bracket main body can fix the embryo transfer tube; one end of the fixing device is higher than the other end of the fixing device, so that the orifice of the fixed embryo transfer tube is inclined upwards, and the embryo is effectively prevented from sliding out of the transfer tube; by means of the triangular supporting structure of the handheld part, medical staff can stably transfer an embryo transfer tube to a patient for an operation, shaking of the embryo transfer tube in the transfer process is effectively avoided, in the transfer process, the constant-temperature box can meet the environment condition for embryo storage, the patient can confirm the embryo through an observation opening, a nurse does not need to take out the transfer tube, and operation is convenient. And sliding-out in the taking-out process is avoided, and the problems that the transfer efficiency is low, and transfer operation is not convenient enough are effectively solved.

An apparatus (100) suitable for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species, comprising a body (110) configured to fit within a lumen of the female reproductive system. The body (110) comprises one or more lumens extending from a proximal end (130) of the body (110) to a distal portion of the body (110) and having a distal opening (150) at the distal portion of the body (110), wherein a first lumen (120) of the one or more lumens slidably receives an inner body (160) having a distal end suitable for penetrating the endometrial epithelium. The inner body (160) comprises an inner lumen for receiving a fertilized egg. An actuator (195) is operable to expel the fertilized egg from the inner lumen. A covering layer (170a) at least partially covers the distal opening of at least one lumen of the one or more lumens and at least partially covers the distal opening (150) of the first lumen (120) and the inner body (160) is configured to perforate through the covering layer (170a) when the inner body (160) is advanced out from the distal opening (150) of the body (110).

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The present disclosure relates to a method for constructing a mouse model with short telomere. Specifically, the present disclosure obtains fertilized eggs by fertilizing the sperms and eggs of mice in vitro, and the fertilized eggs are cultured to the blastocyst stage in vitro and then transferred into the surrogate female mouse for development, thereby producing mice with short telomere. The method of the present disclosure does not require gene editing, has a short modeling cycle, and the effect is reliable and stable. By changing only the environment during embryo transfer, the telomere elongation process in the embryo is interfered, so as to successfully construct a progeny model with short telomere. There is no significant effect on the reproductive rate of female mice. Therefore, the present disclosure can provide a method for constructing a mouse model with short telomere for exploring the mechanism of telomere shortening and studying telomere-related phenotypes such as aging.

The present invention relates to dispensing instruments and methods for introducing treatment material and fluid-like material with an embryo into a uterus. More specifically, the present invention relates to apparatuses for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species and associated computer programs for controlling said apparatuses.

The present invention relates to a two-step dual-station integrated forming machine for all-steel engineering tires, and belongs to the technical field of tire processing equipment. It includes a first stage tire embryo transfer device and a second stage tire embryo transfer device, a transfer trolley and a rotating mechanism are arranged between the two tire embryo transfer devices, the first stage tire embryo transfer device includes a first track and a carcass drum case, a carcass drum is installed on the carcass drum case, a plurality of first feed racks for feeding materials to the carcass drum are arranged on one side of the first track, a carcass barrel transfer mechanism is arranged at the end of the first track, a second feed rack is arranged on the side of the carcass barrel transfer mechanism, the two-stage tire embryo transfer device includes a second track and a belt drum case, a belt drum is installed on the belt drum case, a plurality of belt feed racks are arranged on one side of the second track, and a belt transfer ring is arranged near a belt feed rack located at the end. The present invention can reduce labor costs, improve efficiency, and enhance tire quality.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention discloses a construction method of a Dcaf17 gene modified golden hamster model and application of the Dcaf17 gene modified golden hamster model. The method comprises the following steps: under a red light source, injecting a Cas9 functional element and sgRNA of a targeted Dcaf17 gene exon 1 through a two-cell embryo, and transplanting the embryo into a receptor body, so as to obtain the Dcaf17 gene modified golden hamster. The Dcaf17 gene modified golden hamster model is constructed by utilizing two-cell stage embryo gene editing, and the Dcaf17 gene modified golden hamster homozygous male rat has the phenotype characteristics of no fertilization ability, normal testis and the like, and is used for replacing ligation male rats to prepare false pregnancy receptor rats. The receptor can efficiently identify and utilize a false pregnancy receptor through microscopic examination of sperms by a microscope. Therefore, the invention solves the technical difficulty of receptor preparation in related experiments of assisted reproduction of the golden hamster, and can be used for phenotype research of the Dcaf17 gene and related applications of assisted reproduction of the golden hamster.

The invention discloses a gene editing system for developing a Staphylococcus aureus mastitis resistant Holstein cow aiming at an ADAM10 gene and application of the gene editing system. According to the invention, a novel single-base gene editing ABE technology is used to carry out gene editing on dairy cow fibroblasts, amino acids at main action sites of cell surface receptors ADAM10 of alpha-hemolysin are mutated, and the affinity activity between the two is reduced. Experiments prove that compared with a wild type, the inflammatory sensitivity of the mutated cow mammary epithelial cells to alpha-hemolysin is greatly reduced. Then, gene editing individuals are cultivated through cell nucleus transfer and embryo transfer technologies, and the mastitis resistance of the individuals is achieved.

The invention relates to the technical field of reproductive medicine, and discloses an embryo transfer catheter constant-temperature rinsing device for a reproductive center. The second sliding groove is formed in the top of the middle of the operation box, one end of the second sliding groove is fixedly connected with a first electric push rod, the output end of the first electric push rod is connected with a sliding block, the sliding block is slidably connected with the inner wall of the second sliding groove, one end of the sliding block is fixedly connected with a second electric push rod, and the output end of the second electric push rod is connected with a base. According to the device, a second electric push rod is started, the second electric push rod drives a base to enable a rinsing mechanism to move downwards, the bottom end of an inner pipe is inserted into a transplantation vessel, a third electric push rod is started, the output end of the third electric push rod drives a sliding plate to move, transplantation liquid in the transplantation vessel is repeatedly sucked into the inner pipe, and the purpose of sufficient rinsing is achieved; and the problem that the transplanted embryos are retained in the transplantation catheter due to the fact that the embryos are blocked in the transplantation catheter loading process is solved.

The invention relates to the field of medical instruments, in particular to an embryo transplantation device which comprises a transplantation tube, a push rod is slidably connected to the top end of a cavity in a penetrating mode, an injection tube is fixedly connected to the inner bottom wall of the cavity in a penetrating mode, an adjusting assembly is installed in the injection tube, and an auxiliary assembly is installed in the transplantation tube. The auxiliary assembly is arranged, a connecting pipe is connected with a protective ring, a second control valve is opened, then in the exhaust process of moving a push rod, a second connecting plate and a connecting rod drive a sealing plate to move synchronously, and gas in the gas cavity is exhausted through the pressure difference generated by sealed sliding of the sealing plate and the interior of the gas cavity; the injection port of the movable tube is connected with the connecting tube and enters the protection ring along the connecting tube, so that the protection ring begins to expand until the injection port of the movable tube is completely wrapped, and therefore in the moving process, even if accidental injury occurs, protection can be conducted through the expanded protection ring, accidental injury caused by direct contact with uterine muscular tissue is avoided, and the using effect of the device is improved.