54 results about "Embryo transfer" patented technology

The present application relates to the technical field of warming machine, disclose a kind of embryo inserting device and warming machine.Warming machine includes embryo inserting device and rack, embryo inserting device includes rotating mechanism and several bottom supporting mechanisms, rotating mechanism can be rotated relative to rack around vertical axis, rotating mechanism is provided with several first matching parts along the circumference, and the first matching part is used to limit the upper end of bottle embryo;Several bottom supporting mechanisms are connected with rotating mechanism and are correspondingly arranged below several first matching parts, and bottom supporting mechanism can move upward along vertical direction at preset time, to lift and guide the lower end of bottle embryo located at corresponding first matching part.The warming machine of the present application can ensure that the bottle embryo clamping mechanism is accurately inserted and grasped by setting the above-mentioned embryo inserting device, and improve the yield of bottle embryo processing.

The present disclosure relates to devices and methods for accessing the reproductive tract of an animal, particularly via the animal's vagina, and deploying reproductive material within the uterus. The device and methods of the disclosure may be particularly suited for embryo transfer processes, such as performed in livestock e.g., cattle.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The utility model provides a kind of embryo transfer device with physical locking function belongs to biological sample experimental equipment field.The device includes isolation cabinet, storage box and transmission disc, and storage box and transmission disc are disc-shaped structure, and storage box includes bottom plate and top cover, and transmission disc is rotatably arranged between bottom plate and top cover, and transmission disc is provided with multiple placing cavities along circumference, and top cover is provided with first placement port and second placement port corresponding with placing cavity, and first placement port and second placement port are respectively located at both sides of isolation cabinet, and when first placement port corresponds with one of placing cavities, second placement port is staggered with other placing cavities. The embryo transfer device with physical locking function provided by the utility model embodiment can solve the problem of high risk of medical accidents in the prior art.

The utility model discloses a kind of mechanical hand centering equipment of curing press, belong to tire curing press auxiliary component field, a kind of mechanical hand centering equipment of curing press, including curing press center machine, the upper end of the curing press center machine is fixedly connected with base, the inner wall between the base is equipped with carousel, the upper end of the base is fixedly connected with limit frame, four centering plates are movably connected between the limit frame and carousel, four The centering plate is distributed in cross shape, and laser ranging probe is embedded and installed in outer end, can be transferred to the embryo when curing press center machine is above, the embryo that can be transferred is automatically centered, and offset error value data can also be transmitted to the operating program of mechanical hand, so that mechanical hand secondary embryo transfer, position adjustment can be made in time, reduce subsequent personnel repeatedly loaded embryo position commissioning step, speed up work efficiency.

The invention belongs to the technical field of medical instruments, and provides an embryo transfer catheter auxiliary device which comprises an inner supporting core and a heating wire. The inner supporting core is formed by coaxially connecting a supporting rod and a flexible pipe with a closed front end, the flexible pipe is filled with a phase change material, and the heating wire penetrates through the flexible pipe and is used for heating and softening the material. A containing cavity is formed in the supporting rod, a slidable piston is arranged in the containing cavity, the multiple supporting pieces are connected in series through a connecting rope and connected to the piston, and the farthest end abuts against the closed end of the flexible pipe. The piston moves to drive the supporting piece to enter and exit from the flexible pipe, and dynamic adjustment of the volume of the phase-change material is achieved. The supporting piece is made of an ultrasonic visual material or structure, and intraoperative positioning is facilitated. Due to the adoption of the structure, the device provides rigid support during catheter placement, can actively reduce the volume and soften after positioning, realizes non-traction and low-resistance withdrawal, supports ultrasonic real-time guidance, and remarkably improves the positioning precision and the operation safety of the outer tube.

The present invention relates to dispensing instruments and methods for introducing treatment material and fluid-like material with an embryo into a uterus. More specifically, the present invention relates to apparatuses for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species and associated computer programs for controlling said apparatuses.

The invention discloses a leakproof efficient embryo flushing pipe for horse embryo transplantation, and relates to the technical field of horse breeding instruments.The leakproof efficient embryo flushing pipe comprises a main pipe, the bottom end of the main pipe is communicated with an embryo flushing liquid input pipe and an embryo flushing liquid output pipe, the embryo flushing liquid input pipe is communicated with a pump-out unit, the embryo flushing liquid output pipe is communicated with a recovery unit, and the upper portion of the main pipe is sleeved with an outer pipe; the top end of the main pipe is communicated with two blank flushing pipes respectively; the two blank flushing pipes are folded to form a cylindrical structure and have the same diameter as the outer pipe; driving combining assemblies are arranged on the opposite sides of the two blank flushing pipes and used for driving the two blank flushing pipes to be combined or separated; an air bag plugging assembly and a rubber plugging assembly are sequentially arranged at the end, close to the blank flushing pipe, of the outer pipe from top to bottom. After the two embryo flushing pipes extend into the uterus, the two embryo flushing pipes are separated through the driving combining and separating assembly and face the uterus horns on the two sides respectively, and the flushing and recycling efficiency is greatly improved; the cervix is blocked through the air bag blocking assembly and the rubber blocking assembly, safety and reliability are achieved, and flushing fluid is prevented from leaking out.

The utility model aims to provide a device for safely transferring an embryo transfer tube, which relates to the technical field of medical instruments and is characterized in that an embryo transfer device is designed, so that a bracket main body can be accommodated in a constant-temperature box, and a fixing device on the bracket main body can fix the embryo transfer tube; one end of the fixing device is higher than the other end of the fixing device, so that the orifice of the fixed embryo transfer tube is inclined upwards, and the embryo is effectively prevented from sliding out of the transfer tube; by means of the triangular supporting structure of the handheld part, medical staff can stably transfer an embryo transfer tube to a patient for an operation, shaking of the embryo transfer tube in the transfer process is effectively avoided, in the transfer process, the constant-temperature box can meet the environment condition for embryo storage, the patient can confirm the embryo through an observation opening, a nurse does not need to take out the transfer tube, and operation is convenient. And sliding-out in the taking-out process is avoided, and the problems that the transfer efficiency is low, and transfer operation is not convenient enough are effectively solved.

An apparatus (100) suitable for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species, comprising a body (110) configured to fit within a lumen of the female reproductive system. The body (110) comprises one or more lumens extending from a proximal end (130) of the body (110) to a distal portion of the body (110) and having a distal opening (150) at the distal portion of the body (110), wherein a first lumen (120) of the one or more lumens slidably receives an inner body (160) having a distal end suitable for penetrating the endometrial epithelium. The inner body (160) comprises an inner lumen for receiving a fertilized egg. An actuator (195) is operable to expel the fertilized egg from the inner lumen. A covering layer (170a) at least partially covers the distal opening of at least one lumen of the one or more lumens and at least partially covers the distal opening (150) of the first lumen (120) and the inner body (160) is configured to perforate through the covering layer (170a) when the inner body (160) is advanced out from the distal opening (150) of the body (110).

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention discloses a construction method of a Dcaf17 gene modified golden hamster model and application of the Dcaf17 gene modified golden hamster model. The method comprises the following steps: under a red light source, injecting a Cas9 functional element and sgRNA of a targeted Dcaf17 gene exon 1 through a two-cell embryo, and transplanting the embryo into a receptor body, so as to obtain the Dcaf17 gene modified golden hamster. The Dcaf17 gene modified golden hamster model is constructed by utilizing two-cell stage embryo gene editing, and the Dcaf17 gene modified golden hamster homozygous male rat has the phenotype characteristics of no fertilization ability, normal testis and the like, and is used for replacing ligation male rats to prepare false pregnancy receptor rats. The receptor can efficiently identify and utilize a false pregnancy receptor through microscopic examination of sperms by a microscope. Therefore, the invention solves the technical difficulty of receptor preparation in related experiments of assisted reproduction of the golden hamster, and can be used for phenotype research of the Dcaf17 gene and related applications of assisted reproduction of the golden hamster.

The invention discloses a gene editing system for developing a Staphylococcus aureus mastitis resistant Holstein cow aiming at an ADAM10 gene and application of the gene editing system. According to the invention, a novel single-base gene editing ABE technology is used to carry out gene editing on dairy cow fibroblasts, amino acids at main action sites of cell surface receptors ADAM10 of alpha-hemolysin are mutated, and the affinity activity between the two is reduced. Experiments prove that compared with a wild type, the inflammatory sensitivity of the mutated cow mammary epithelial cells to alpha-hemolysin is greatly reduced. Then, gene editing individuals are cultivated through cell nucleus transfer and embryo transfer technologies, and the mastitis resistance of the individuals is achieved.

The utility model relates to a device for improving embryo culture dish transfer efficiency and safety, which comprises an incubator arranged on a table top in an IVF (in-vitro fertilization) workstation and a box cover arranged on the incubator and capable of opening and closing the incubator, the incubator is arranged on the right side of a stereoscopic microscope fixing column and is positioned in front of the right of a heating plate below an objective lens of a stereoscopic microscope, and the box cover is arranged on the right side of the stereoscopic microscope fixing column. The bottom of the IVF work station is provided with a pedal auxiliary switch used for controlling opening and closing of the box cover. The box cover comprises an overweight box cover body, a lever fixedly connected with the overweight box cover body, a linkage rod rotationally connected to the tail of the lever, a vertical rod rotationally connected to the tail of the linkage rod and capable of vertically moving, a cam block used for driving the vertical rod to move up and down, a mounting shell used for mounting the cam block and a servo motor used for driving the cam block. The incubator arranged on the table board is arranged in the table board of the IVF work station, and a pedal switch is treaded by feet to control the box cover, so that two hands are liberated, and the speed and the safety of transferring a culture dish between the incubator and the stereoscope heating table are improved.

The utility model relates to the technical field of bottle production lines, in particular to a rotary bottle blowing machine. The rotary bottle blowing machine is provided with a guide assembly, the guide assembly comprises a first guide plate and a second guide plate, the first guide plate is installed above the protective plate, the second guide plate is located above the transfer gear, the first guide plate and the second guide plate are arranged in a spaced mode to form a guide gap, and the part, above the supporting ring, of the bottle preform can be clamped in the guide gap. The upper portion of the bottle preform supporting ring is fixed in the guide gap, so that the single fixing mode of the bottle preform on the lower side of the supporting ring is changed into the double fixing mode of the upper side and the lower side of the supporting ring, the stability of the bottle preform in the transferring process is improved, and the bottle preform is prevented from shaking left and right; and the axial line of the bottle blank and the extending direction of the heating head are parallel to the rotating axial direction of the transfer assembly, so that the accuracy and success rate of inserting the heating head into the bottle blank are improved, and the bottle blank is prevented from being damaged.

The utility model discloses a kind of hair-planting and hair-trimming combination machines, including brush embryo conveying mechanism, manipulator, brush embryo transfer mechanism, hair-planting machine and hair-trimming machine, the brush embryo transfer mechanism carries out horizontal transport to brush embryo, the hair-planting machine and hair-trimming machine are located at the same side of brush embryo transfer mechanism moving route, the brush embryo conveying mechanism and manipulator are located at the side of brush embryo transfer mechanism back to hair-trimming machine, the manipulator is gripped and conveyed to brush embryo transfer mechanism by brush embryo conveying mechanism on brush embryo, the brush embryo transfer mechanism is horizontally conveyed to hair-planting machine and hair-trimming machine, brush embryo conveying mechanism is conveyed to conveying end then under the action of manipulator is transferred to brush embryo transfer mechanism, brush embryo transfer mechanism horizontal conveying realizes the automatic conveying of brush embryo between hair-planting machine and hair-trimming machine, realizes the good automatic conveying of brush embryo, effectively reduces artificial, and improve production efficiency, good realization brush embryo's automatic hair-planting and automatic hair-trimming.

Disclosed in the present invention are a composition and method for increasing the number and proportion of inner cell mass cells of an in vitro embryo and use. According to the present invention, in view of the problems of in vitro fertilized embryos such as low blastocyst rate during early development, low inner cell mass cell number in the blastocyst stage, low proportion of the inner cell mass cells, and poor subsequent embryonic development potential, by adding one or two of regulators folic acid and laxiflorin B into the culture medium for early embryo in vitro culturing, the blastocyst rate, the number and proportion of the inner cell mass cells in the blastocyst, the survival rate of blastocysts after extended culturing, and the diameter of blastocysts are significantly improved, effectively improving the developmental potential of in vitro embryos. In addition, the present invention also effectively improves the ratio of in vitro fertilized embryos that can be subsequently transplanted. The present invention is low in cost, safe, and efficient, provides a new strategy for in vitro embryo production of livestock and development of human-assisted reproductive technology, and exhibits broad application prospects.

The application discloses a menstrual blood organoid and application thereof, and belongs to the technical field of organoid construction. The menstrual blood organoid is preserved in the China Center for Type Culture Collection, is named as human menstrual blood organoid MFO-Jennifer (Homo sapiens), has a preservation number of CCTCC NO: C2024133, and is preserved on September 25, 2024. The menstrual blood organoid is applied to construction of an embryo implantation model, construction of an individualized drug screening model, and construction of a receptivity test model of in vitro fertilization-embryo transfer. The application establishes a method for inducing the menstrual blood organoid to an implantation window stage, and provides a powerful research tool for studying changes of endometrium during embryo implantation and interaction of a maternal-fetal interface.

The invention discloses an embryo vitrification device and belongs to the field of assisted reproduction, a liquid suction and spitting area of a vitrification vessel is communicated with a balance area, a collection area is of a through groove structure, a liquid suction structure is connected with the bottom of the collection area, and a slide glass is located between the bottom of the collection area and the liquid suction structure. The pressing structure is installed on the support and abuts against the vitrification vessel so that the collecting area and the slide glass can be pressed tightly, the liquid suction structure sucks liquid in the collecting area so that embryos can fall to the slide glass, and the microscopic observation structure collects images of the balance area and the collecting area so as to observe the embryo shapes, through the design, liquid suction is carried out in the liquid suction and spit area, the embryos in the balance area can be prevented from being sucked away, and the embryo quality is improved. The embryo loss is caused; the balance area is separated from the collection area, so that imaging is facilitated, and embryo transfer is also facilitated; the liquid suction structure sucks away liquid in the collection area, embryos fall on the slide glass and are convenient to collect, the microscopic observation structure automatically observes embryo forms, and the automation degree is high.

The invention includes a method of identifying high genomic value, non-human mammalian embryos that would otherwise be disposed of or frozen based on their morphology. The method generally involves establishing a stem cell culture from the non-human mammalian embryo, which entails generating a population of embryos in vitro or in vivo, assessing the morphology of each of the embryos in the population, selecting a subpopulation of embryos for embryo transfer based on the morphological assessment, thereby establishing a group of selected embryos and a group of unselected embryos in the population, and establishing a stem cell culture from an embryo in the group of unselected embryos.

The utility model discloses a fixing frame for embryo transplantation cattle, which comprises a base, a fixing frame is fixedly mounted at the top of the base, fixing plates are symmetrically mounted on the inner side of the fixing frame, threaded rods are in threaded connection with the inner centers of the two fixing plates, movable frames are rotationally connected with the sides, close to each other, of the two threaded rods, and the movable frames are in threaded connection with the fixing plates. Guide rods are symmetrically mounted on the sides, away from each other, of the two movable frames, the guide rods are slidably connected with the fixing plate, a positioning assembly is arranged on one side of the fixing frame and comprises mounting frames symmetrically mounted at the top of the fixing frame, and L-shaped frames are symmetrically mounted on one side of the mounting frame on the left side; a supporting rod is fixedly mounted between the two L-shaped frames; by means of the arrangement, when embryo transplantation cattle are diagnosed or treated, the heads of cattle of different figures can be fixed, the fixing positions of the heads can be conveniently adjusted, the application range is widened, and discomfort of the cattle is avoided.

Methods and formulations for a simplified, single-injection method to induce and control the synchronous growth (superstimulation), and ovulation (superovulation) of multiple ovarian follicles in bovine, ovine, caprine, camelid and other female animals enabling the subsequent collection of (a) multiple oocytes when conducting in-vitro fertilization, or (b) multiple embryos when conducting multiple ovulation embryo transfer.

This invention discloses a method for constructing and applying an anti-obesity animal model based on ISCA1 / 2 gene regulation, belonging to the field of biomedical technology. The method utilizes CRISPR-Cas9 gene editing technology to design specific single-stranded guide RNAs targeting the ISCA1 and ISCA2 genes in mice. Cas9 mRNA and sgRNA are introduced into fertilized eggs of C57BL / 6J strain mice via microinjection. The injected embryos are then transferred into pseudopregnant mice to obtain F0 generation chimeric mice. By mating F0 generation mice with wild-type C57BL / 6J mice, F1 generation heterozygous mice carrying the target mutation in the germline are selected, thus obtaining the anti-obesity animal model. This model is not only a phenotypic model but also a mechanistic research model, suitable for drug target validation, energy metabolism pathway exploration, and personalized treatment strategy evaluation.

The invention discloses a method for rapidly constructing a Foxn1 homozygous nude mouse through RepSox induction based on CRISPR / Cas9, and the method comprises the following steps: synthesizing two sgRNAs for respectively identifying a 5'terminal target site and a 3 'terminal target site, the nucleotide sequence of the sgRNA for identifying the 5' terminal target site is as shown in SEQ ID NO: 1, and the nucleotide sequence of the sgRNA for identifying the 3 'terminal target site is as shown in SEQ ID NO: 2; a mixed solution of the two sgRNAs, the Cas protein and the RepSox is microinjected into the fertilized eggs of the rats; the injected embryos are subjected to transient in-vitro culture, survival embryos are screened and transplanted into fallopian tubes or uteruses of pseudopregnant female rats for development, and F0-generation Foxn1 knockout homozygous rats are obtained. According to the method, the Foxn1 gene can be accurately knocked out, the survival rate of a gene editing embryo is improved, the model construction period is shortened, and the rat knockout efficiency is remarkably improved.

The invention discloses a library building method for detecting G-quadruplex structures in oocytes, early embryos and trace cells, and belongs to the technical field of whole genome immunoprecipitation library building. The DNA G-quadruplex structure provided by the invention is a DNA secondary structure which is different from a classic double-helix structure; g-quadruplex structure abnormity can damage telomere stability, genome stability, transcriptional activity and the like; this is an important reason for poor ovum quality and embryonic development retardation. The method provided by the invention can be used for evaluating the quality of the ova of the infertile women and judging reasons causing immaturity of the ova; abnormal enrichment of in-vivo and in-vitro development arrest embryo whole genome G-quadruplex is detected and can be used as an important index for screening reasons causing embryonic development retardation; the method can be used for guiding whether corresponding clinical patients are suitable for a series of assisted reproductive technologies (ART) such as in vitro fertilization-embryo transfer (IVF-ET) and the like.

This invention relates to a visual embryo transfer catheter, comprising a catheter portion including a catheter, a support tube, a first seat, a sensing assembly, and a display screen. The support tube is disposed on one side of the catheter. The first seat is disposed at one end of the support tube. The catheter lumen communicates with a cavity on the first seat. An annular cavity is formed on the wall of the catheter. The sensing portion of the sensing assembly extends into the annular cavity and can monitor at least a section of the catheter lumen near its free end. The sensing assembly is connected to the display screen to display the monitored condition within the catheter lumen. This invention enables rapid confirmation during embryo transfer of whether the embryo has been adequately pushed into the uterus, thus helping to shorten the time the catheter remains in the uterus and reducing uterine stimulation during embryo transfer.

The utility model discloses a kind of brush embryo transfer mechanism, including main body, the bottom of the main body has the sliding block for horizontal sliding connection, swing arm is rotatably connected on the main body, the end of the swing arm is provided with clamp for clamping brush embryo, the first drive source for driving swing arm rotation is arranged on the main body, clamp on main body can be used to clamp brush embryo, then by the second drive source driving main body horizontal movement realizes the transport of brush embryo, after clamping brush embryo, it can be horizontally transported to the place of hair-planting machine and hair trimming machine, by the first drive source, swing arm can be driven to rotate to realize the steering of before and after, facilitate the grasping and placement of brush embryo, realize the good automatic transport of brush embryo, effectively reduce artificial, improve production efficiency.

The double-station forming equipment comprises a first station and a second station which are arranged in parallel, transfer joint robots are arranged at the ends of the first station and the second station, and an edge cutting device and a piece taking / stacking mechanical arm are arranged on the side face of the second station. Each of the first station and the second station comprises a slurry fishing forming device, a hot press forming device and a corresponding wet blank transfer manipulator which operate independently, the production procedures of the first station and the second station are the same, and the difference is that the starting time of slurry fishing forming is different, so that the finishing time of the hot press forming procedure can be staggered; and the transfer robot can be quickly connected to the dry blank taking and transferring of the station II after completing the hot-pressing dry blank taking of the station I and transferring the hot-pressing dry blank to the edge cutting device. The production method comprises the steps of double-station independent slurry suction forming, wet blank transferring, hot-pressing shaping, edge cutting and taking and stacking. The problem that the production cycle is limited by the pulp fishing forming time when a single fishing pool corresponds to single hot pressing or double hot pressing forming is solved, and the productivity is improved.