73 results about "Embryo transfer" patented technology

The embryo transfer device comprises a clamp, a carrying rod and a sleeve, the clamp is provided with a structure used for installing the carrying rod and the sleeve, the carrying rod and the sleeve are matched with the structure in an embedded mode, the carrying rod and / or the sleeve and components on the structure generate acceleration through relative acting force, and the clamping device is used for clamping the carrying rod and / or the sleeve and the components on the structure. And the embryo on the carrying rod falls into the sleeve. The embryo transfer can be completed without a microscope and a mouth suction tube, and the embryo transfer device is convenient to use in pastures and remote environments. According to the embryo transfer device, embryo transfer can be completed without a microscope and a mouth suction tube, the transferred embryo is almost free of damage, the situation that the embryo is lost in the transfer process is avoided to the maximum extent, and the embryo transfer device further has the advantages of being simple in structure, easy to implement, easy to operate, low in cost and the like.

The present application relates to the technical field of warming machine, disclose a kind of embryo inserting device and warming machine.Warming machine includes embryo inserting device and rack, embryo inserting device includes rotating mechanism and several bottom supporting mechanisms, rotating mechanism can be rotated relative to rack around vertical axis, rotating mechanism is provided with several first matching parts along the circumference, and the first matching part is used to limit the upper end of bottle embryo;Several bottom supporting mechanisms are connected with rotating mechanism and are correspondingly arranged below several first matching parts, and bottom supporting mechanism can move upward along vertical direction at preset time, to lift and guide the lower end of bottle embryo located at corresponding first matching part.The warming machine of the present application can ensure that the bottle embryo clamping mechanism is accurately inserted and grasped by setting the above-mentioned embryo inserting device, and improve the yield of bottle embryo processing.

The construction of a non-human primate Usher syndrome disease model having USH2A gene mutation, wherein a target sequence against the mRNA of the non-human primate USH2A gene is established and a corresponding sgRNA is synthesized, then a knockout vector is constructed, the sgRNA and a Cas9 mRNA are mixed and injected into a non-human primate embryo to obtain a non-human primate embryo having USH2A gene knockout, and then by means of embryo transfer, a non-human primate having USH2A gene knockout is obtained, which exhibits symptoms of Usher syndrome. Upon detection and identification of the knockout effect, the knockout of the USH2A gene can be effectively realized in a non-human primate cynomolgus monkey embryo and cynomolgus monkeys, which provides a new and effective disease model for the diagnosis and treatment of Usher syndrome in non-human primates.

The embryo transfer device comprises a clamp, structures and mechanisms, the structures are arranged on the clamp and used for containing embryos, the mechanisms are used for enabling the embryos to fall off from the structures, and the mechanisms apply acceleration to the structures to enable the embryos to fall off from the structures into containers. The invention also comprises an embryo transfer method which comprises the following steps: placing an embryo on a structure; placing the structure in a container; acceleration is applied to the structure manually or through a mechanism, so that the cells fall off from the structure into the container. According to the invention, cells on the structure are transferred into the container by shifting the structure, embryo transfer can be completed without a microscope and a mouth suction tube, and the device is convenient to use in pastures and remote environments. The transferred embryos are almost free of damage, the situation that the embryos are lost in the transferring process is avoided to the maximum extent, and the embryo transferring device further has the advantages of being simple in structure, easy to implement, easy to operate, low in cost and the like.

The present disclosure relates to devices and methods for accessing the reproductive tract of an animal, particularly via the animal's vagina, and deploying reproductive material within the uterus. The device and methods of the disclosure may be particularly suited for embryo transfer processes, such as performed in livestock e.g., cattle.

The utility model discloses a multi-cavity continuous heating bottle blowing machine pitch changing mechanism which comprises a plurality of blank feeding guide rail cushion blocks, a blank moving X air cylinder seat is installed on the tops of the blank feeding guide rail cushion blocks, a first air cylinder is installed on the blank moving X air cylinder seat, a first linear sliding block guide rail is installed on the tops of the blank feeding guide rail cushion blocks, and a second linear sliding block guide rail is installed on the linear sliding block guide rail. A blank moving X driving seat is mounted on an upper slide way of the linear slide block guide rail I, and the output end of the air cylinder I is connected with the blank moving X driving seat; a blank moving Y-shaped material is installed on the top of the blank moving X-shaped driving base, a blank moving Y-shaped air cylinder base is installed on the blank moving Y-shaped material, a front blank moving block and a rear blank moving block are installed on the blank moving Y-shaped material in a sliding way, and the front blank moving block and the rear blank moving block are installed on the blank moving Y-shaped material through a blank moving Y-shaped driving base sliding way. The stroke of the second air cylinder and the stroke of the third air cylinder are different, two bottle blanks are pulled apart by a certain distance (bottle blowing distance) from the initial adjacent distance through the second air cylinder and the third air cylinder, the distance changing function is achieved, the structure is simple, and operation is easy.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The utility model provides a kind of embryo transfer device with physical locking function belongs to biological sample experimental equipment field.The device includes isolation cabinet, storage box and transmission disc, and storage box and transmission disc are disc-shaped structure, and storage box includes bottom plate and top cover, and transmission disc is rotatably arranged between bottom plate and top cover, and transmission disc is provided with multiple placing cavities along circumference, and top cover is provided with first placement port and second placement port corresponding with placing cavity, and first placement port and second placement port are respectively located at both sides of isolation cabinet, and when first placement port corresponds with one of placing cavities, second placement port is staggered with other placing cavities. The embryo transfer device with physical locking function provided by the utility model embodiment can solve the problem of high risk of medical accidents in the prior art.

The purpose of the present invention is to provide an excellent infertility treatment capable of improving the implantation rate of an embryo (fertilized egg) by improving the uterus environment. The present invention is a composition for treating uterus, which contains a mesenchymal stem cell culture supernatant, and which is used as a uterus environment-improving agent, an implantation aid, an embryo transplantation solution, a sperm transplantation solution or an implantation rate-improving agent. The mesenchymal stem cells are preferably derived from adipose tissue, umbilical cord tissue or bone marrow tissue.

The utility model discloses a kind of mechanical hand centering equipment of curing press, belong to tire curing press auxiliary component field, a kind of mechanical hand centering equipment of curing press, including curing press center machine, the upper end of the curing press center machine is fixedly connected with base, the inner wall between the base is equipped with carousel, the upper end of the base is fixedly connected with limit frame, four centering plates are movably connected between the limit frame and carousel, four The centering plate is distributed in cross shape, and laser ranging probe is embedded and installed in outer end, can be transferred to the embryo when curing press center machine is above, the embryo that can be transferred is automatically centered, and offset error value data can also be transmitted to the operating program of mechanical hand, so that mechanical hand secondary embryo transfer, position adjustment can be made in time, reduce subsequent personnel repeatedly loaded embryo position commissioning step, speed up work efficiency.

This embryo transfer device kit comprises: a connection component (2) which is connected to the base end of a tube the leading end thereof being disposed inside a uterus and the base end thereof disposed outside the uterus; and an embryo transfer device (1), at least a portion of which is housed in a housing unit (71A) that is provided to the connection component (2) so as to extend in a prescribed direction. The embryo transfer device (1) is provided with an embryo storage unit (20) having a recess (21) that opens in a direction intersecting with the prescribed direction. In a state in which the embryo storage unit (20) is disposed in a uterine cavity, the embryo storage unit holds an embryo, which is disposed in the recess (21), in a space surrounded by the recess (21) and the endometrium of the uterine cavity which is present in the opening direction of the recess (21).

The invention belongs to the technical field of medical instruments, and provides an embryo transfer catheter auxiliary device which comprises an inner supporting core and a heating wire. The inner supporting core is formed by coaxially connecting a supporting rod and a flexible pipe with a closed front end, the flexible pipe is filled with a phase change material, and the heating wire penetrates through the flexible pipe and is used for heating and softening the material. A containing cavity is formed in the supporting rod, a slidable piston is arranged in the containing cavity, the multiple supporting pieces are connected in series through a connecting rope and connected to the piston, and the farthest end abuts against the closed end of the flexible pipe. The piston moves to drive the supporting piece to enter and exit from the flexible pipe, and dynamic adjustment of the volume of the phase-change material is achieved. The supporting piece is made of an ultrasonic visual material or structure, and intraoperative positioning is facilitated. Due to the adoption of the structure, the device provides rigid support during catheter placement, can actively reduce the volume and soften after positioning, realizes non-traction and low-resistance withdrawal, supports ultrasonic real-time guidance, and remarkably improves the positioning precision and the operation safety of the outer tube.

The present invention relates to dispensing instruments and methods for introducing treatment material and fluid-like material with an embryo into a uterus. More specifically, the present invention relates to apparatuses for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species and associated computer programs for controlling said apparatuses.

The invention discloses a leakproof efficient embryo flushing pipe for horse embryo transplantation, and relates to the technical field of horse breeding instruments.The leakproof efficient embryo flushing pipe comprises a main pipe, the bottom end of the main pipe is communicated with an embryo flushing liquid input pipe and an embryo flushing liquid output pipe, the embryo flushing liquid input pipe is communicated with a pump-out unit, the embryo flushing liquid output pipe is communicated with a recovery unit, and the upper portion of the main pipe is sleeved with an outer pipe; the top end of the main pipe is communicated with two blank flushing pipes respectively; the two blank flushing pipes are folded to form a cylindrical structure and have the same diameter as the outer pipe; driving combining assemblies are arranged on the opposite sides of the two blank flushing pipes and used for driving the two blank flushing pipes to be combined or separated; an air bag plugging assembly and a rubber plugging assembly are sequentially arranged at the end, close to the blank flushing pipe, of the outer pipe from top to bottom. After the two embryo flushing pipes extend into the uterus, the two embryo flushing pipes are separated through the driving combining and separating assembly and face the uterus horns on the two sides respectively, and the flushing and recycling efficiency is greatly improved; the cervix is blocked through the air bag blocking assembly and the rubber blocking assembly, safety and reliability are achieved, and flushing fluid is prevented from leaking out.

The utility model aims to provide a device for safely transferring an embryo transfer tube, which relates to the technical field of medical instruments and is characterized in that an embryo transfer device is designed, so that a bracket main body can be accommodated in a constant-temperature box, and a fixing device on the bracket main body can fix the embryo transfer tube; one end of the fixing device is higher than the other end of the fixing device, so that the orifice of the fixed embryo transfer tube is inclined upwards, and the embryo is effectively prevented from sliding out of the transfer tube; by means of the triangular supporting structure of the handheld part, medical staff can stably transfer an embryo transfer tube to a patient for an operation, shaking of the embryo transfer tube in the transfer process is effectively avoided, in the transfer process, the constant-temperature box can meet the environment condition for embryo storage, the patient can confirm the embryo through an observation opening, a nurse does not need to take out the transfer tube, and operation is convenient. And sliding-out in the taking-out process is avoided, and the problems that the transfer efficiency is low, and transfer operation is not convenient enough are effectively solved.

An apparatus (100) suitable for delivering a fertilized egg or embryo into a maternal uterine endometrium in humans or any other mammalian species, comprising a body (110) configured to fit within a lumen of the female reproductive system. The body (110) comprises one or more lumens extending from a proximal end (130) of the body (110) to a distal portion of the body (110) and having a distal opening (150) at the distal portion of the body (110), wherein a first lumen (120) of the one or more lumens slidably receives an inner body (160) having a distal end suitable for penetrating the endometrial epithelium. The inner body (160) comprises an inner lumen for receiving a fertilized egg. An actuator (195) is operable to expel the fertilized egg from the inner lumen. A covering layer (170a) at least partially covers the distal opening of at least one lumen of the one or more lumens and at least partially covers the distal opening (150) of the first lumen (120) and the inner body (160) is configured to perforate through the covering layer (170a) when the inner body (160) is advanced out from the distal opening (150) of the body (110).

The present invention relates to means and methods for in vitro fertilisation, particularly to means and methods for frozen embryo transfer which comprise the use of a composition comprising an effective amount of estetrol component. The composition is intended for use prior, during and / or after embryo transfer. The use of the composition in a frozen embryo transfer fertility treatment results in favourable effects when compared to compositions used in the art to support frozen embryo transfer fertility treatments.

The present disclosure relates to a method for constructing a mouse model with short telomere. Specifically, the present disclosure obtains fertilized eggs by fertilizing the sperms and eggs of mice in vitro, and the fertilized eggs are cultured to the blastocyst stage in vitro and then transferred into the surrogate female mouse for development, thereby producing mice with short telomere. The method of the present disclosure does not require gene editing, has a short modeling cycle, and the effect is reliable and stable. By changing only the environment during embryo transfer, the telomere elongation process in the embryo is interfered, so as to successfully construct a progeny model with short telomere. There is no significant effect on the reproductive rate of female mice. Therefore, the present disclosure can provide a method for constructing a mouse model with short telomere for exploring the mechanism of telomere shortening and studying telomere-related phenotypes such as aging.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention discloses a construction method of a Dcaf17 gene modified golden hamster model and application of the Dcaf17 gene modified golden hamster model. The method comprises the following steps: under a red light source, injecting a Cas9 functional element and sgRNA of a targeted Dcaf17 gene exon 1 through a two-cell embryo, and transplanting the embryo into a receptor body, so as to obtain the Dcaf17 gene modified golden hamster. The Dcaf17 gene modified golden hamster model is constructed by utilizing two-cell stage embryo gene editing, and the Dcaf17 gene modified golden hamster homozygous male rat has the phenotype characteristics of no fertilization ability, normal testis and the like, and is used for replacing ligation male rats to prepare false pregnancy receptor rats. The receptor can efficiently identify and utilize a false pregnancy receptor through microscopic examination of sperms by a microscope. Therefore, the invention solves the technical difficulty of receptor preparation in related experiments of assisted reproduction of the golden hamster, and can be used for phenotype research of the Dcaf17 gene and related applications of assisted reproduction of the golden hamster.

The invention discloses a gene editing system for developing a Staphylococcus aureus mastitis resistant Holstein cow aiming at an ADAM10 gene and application of the gene editing system. According to the invention, a novel single-base gene editing ABE technology is used to carry out gene editing on dairy cow fibroblasts, amino acids at main action sites of cell surface receptors ADAM10 of alpha-hemolysin are mutated, and the affinity activity between the two is reduced. Experiments prove that compared with a wild type, the inflammatory sensitivity of the mutated cow mammary epithelial cells to alpha-hemolysin is greatly reduced. Then, gene editing individuals are cultivated through cell nucleus transfer and embryo transfer technologies, and the mastitis resistance of the individuals is achieved.

The invention relates to the field of medical instruments, in particular to an embryo transplantation device which comprises a transplantation tube, a push rod is slidably connected to the top end of a cavity in a penetrating mode, an injection tube is fixedly connected to the inner bottom wall of the cavity in a penetrating mode, an adjusting assembly is installed in the injection tube, and an auxiliary assembly is installed in the transplantation tube. The auxiliary assembly is arranged, a connecting pipe is connected with a protective ring, a second control valve is opened, then in the exhaust process of moving a push rod, a second connecting plate and a connecting rod drive a sealing plate to move synchronously, and gas in the gas cavity is exhausted through the pressure difference generated by sealed sliding of the sealing plate and the interior of the gas cavity; the injection port of the movable tube is connected with the connecting tube and enters the protection ring along the connecting tube, so that the protection ring begins to expand until the injection port of the movable tube is completely wrapped, and therefore in the moving process, even if accidental injury occurs, protection can be conducted through the expanded protection ring, accidental injury caused by direct contact with uterine muscular tissue is avoided, and the using effect of the device is improved.

The utility model relates to a device for improving embryo culture dish transfer efficiency and safety, which comprises an incubator arranged on a table top in an IVF (in-vitro fertilization) workstation and a box cover arranged on the incubator and capable of opening and closing the incubator, the incubator is arranged on the right side of a stereoscopic microscope fixing column and is positioned in front of the right of a heating plate below an objective lens of a stereoscopic microscope, and the box cover is arranged on the right side of the stereoscopic microscope fixing column. The bottom of the IVF work station is provided with a pedal auxiliary switch used for controlling opening and closing of the box cover. The box cover comprises an overweight box cover body, a lever fixedly connected with the overweight box cover body, a linkage rod rotationally connected to the tail of the lever, a vertical rod rotationally connected to the tail of the linkage rod and capable of vertically moving, a cam block used for driving the vertical rod to move up and down, a mounting shell used for mounting the cam block and a servo motor used for driving the cam block. The incubator arranged on the table board is arranged in the table board of the IVF work station, and a pedal switch is treaded by feet to control the box cover, so that two hands are liberated, and the speed and the safety of transferring a culture dish between the incubator and the stereoscope heating table are improved.

The purpose of the present invention is to provide a culture medium for immature eggs that is excellent in quality and safety and that is capable of efficiently maturing immature eggs with respect to immature eggs for vitro maturation-in vitro fertilization-embryo transfer method (IVM-IVF). The present invention is a composition that is for treating immature eggs and / or embryos (fertilized eggs) and that contains a serum-free mesenchymal stem cell culture medium. The treatment composition is suitably used as an immature egg culture agent, an embryo (fertilized egg) culture agent, an implantation adjuvant, an embryo transfer liquid or an implantation rate improver.

An embryo transfer device (1) has a proximal end (1A) and a distal end (1B) and is inserted into the uterine cavity from the distal end (1B) side using a tube (P) which is inserted into the uterine cervix (220) from the tip thereof. The embryo transfer device (1) is provided with: an embryo container (20) capable of accommodating embryos; and an embryo container guide (60) disposed closer to the distal end (1B) than the embryo container (20). Upon exiting from the tube (P), the embryo container guide (60) expands in a direction away from the center axis so that the embryo container (20) upon exiting from the tube (P) does not rotate about the center axis on the tip side of the tube (P).

The utility model relates to the technical field of bottle production lines, in particular to a rotary bottle blowing machine. The rotary bottle blowing machine is provided with a guide assembly, the guide assembly comprises a first guide plate and a second guide plate, the first guide plate is installed above the protective plate, the second guide plate is located above the transfer gear, the first guide plate and the second guide plate are arranged in a spaced mode to form a guide gap, and the part, above the supporting ring, of the bottle preform can be clamped in the guide gap. The upper portion of the bottle preform supporting ring is fixed in the guide gap, so that the single fixing mode of the bottle preform on the lower side of the supporting ring is changed into the double fixing mode of the upper side and the lower side of the supporting ring, the stability of the bottle preform in the transferring process is improved, and the bottle preform is prevented from shaking left and right; and the axial line of the bottle blank and the extending direction of the heating head are parallel to the rotating axial direction of the transfer assembly, so that the accuracy and success rate of inserting the heating head into the bottle blank are improved, and the bottle blank is prevented from being damaged.

The utility model discloses a kind of hair-planting and hair-trimming combination machines, including brush embryo conveying mechanism, manipulator, brush embryo transfer mechanism, hair-planting machine and hair-trimming machine, the brush embryo transfer mechanism carries out horizontal transport to brush embryo, the hair-planting machine and hair-trimming machine are located at the same side of brush embryo transfer mechanism moving route, the brush embryo conveying mechanism and manipulator are located at the side of brush embryo transfer mechanism back to hair-trimming machine, the manipulator is gripped and conveyed to brush embryo transfer mechanism by brush embryo conveying mechanism on brush embryo, the brush embryo transfer mechanism is horizontally conveyed to hair-planting machine and hair-trimming machine, brush embryo conveying mechanism is conveyed to conveying end then under the action of manipulator is transferred to brush embryo transfer mechanism, brush embryo transfer mechanism horizontal conveying realizes the automatic conveying of brush embryo between hair-planting machine and hair-trimming machine, realizes the good automatic conveying of brush embryo, effectively reduces artificial, and improve production efficiency, good realization brush embryo's automatic hair-planting and automatic hair-trimming.

Disclosed in the present application is an embryo transfer device, comprising a clamp, a loading rod and a sheath, wherein the clamp is provided with a structure for mounting the loading rod and the sheath, the loading rod and the sheath are each in embedded-fit with the structure, and acceleration is generated between the loading rod and / or the sheath and a component on the structure by means of a relative acting force, so as to make an embryo on the loading rod fall into the sheath. Embryo transfer can be completed without requiring a microscope and a straw, thus facilitating the use of the embryo transfer device in a ranch and remote areas. In the present application, embryo transfer can be completed without requiring a microscope and a straw, and the transferred embryo is almost damage free, thereby reducing the chance of embryo loss during the transfer process to the maximum extent. The present application also has the characteristics of a simple structure, easy implementation, simple operation, low cost, etc.

The invention discloses an embryo transplantation outcome prediction method, device, equipment, medium and product, and relates to the field of embryo transplantation, the method comprises the following steps: obtaining historical transplantation data of an embryo transplantation patient; screening and dividing the historical transplantation data to obtain a data set; the data set comprises a training set and an external verification set; performing feature screening on the data set to obtain risk factors; modeling by using the risk factors of the training set and the historical embryo transplantation outcome of the training set to obtain a prediction model; adjusting model parameters of the prediction model by using the external verification set to obtain an adjusted prediction model; and performing prediction by using the adjusted prediction model according to clinical characteristic variables of a pre-embryo transplantation patient to obtain an embryo transplantation outcome. The prediction accuracy of the embryo transplantation outcome is improved.

Disclosed in the present invention are a composition and method for increasing the number and proportion of inner cell mass cells of an in vitro embryo and use. According to the present invention, in view of the problems of in vitro fertilized embryos such as low blastocyst rate during early development, low inner cell mass cell number in the blastocyst stage, low proportion of the inner cell mass cells, and poor subsequent embryonic development potential, by adding one or two of regulators folic acid and laxiflorin B into the culture medium for early embryo in vitro culturing, the blastocyst rate, the number and proportion of the inner cell mass cells in the blastocyst, the survival rate of blastocysts after extended culturing, and the diameter of blastocysts are significantly improved, effectively improving the developmental potential of in vitro embryos. In addition, the present invention also effectively improves the ratio of in vitro fertilized embryos that can be subsequently transplanted. The present invention is low in cost, safe, and efficient, provides a new strategy for in vitro embryo production of livestock and development of human-assisted reproductive technology, and exhibits broad application prospects.