Method for pig in-vitro fertilization and embryo transplantation

Abstract

The invention discloses a method for pig in-vitro fertilization and embryo transplantation, which comprises the following steps: oocyte preparation; sperm capacitation; adjustment of sperm concentration through adding sperm receiving fluid and capacitation through placing the adjusted mixture into an incubator; in-vitro fertilization and in-vitro oosperm culture; and embryo transplantation which adopts an embryo transplantation tube for transplantation. The method for pig in-vitro fertilization and embryo transplantation provided by the invention can be operated simply, the in-vitro fertilization and embryo transplantation can be realized without surgery, no damage can be caused to the pig, the success rate is high, the cost is low, and the method has great significance for promotion of pig in-vitro fertilization, pig cloning and transgenic embryo research and production.

Description

technical field [0001] The invention relates to a method for artificial reproduction of animals, in particular to a pig in vitro fertilization method and an embryo transplantation method. Background technique [0002] Animal embryo transplantation is also called fertilized egg transplantation or gestation through the abdomen. It refers to taking out the early embryos after mating of a fine-bred female animal, or transplanting embryos obtained by in vitro fertilization or other methods into the uterus of another female animal with the same physiological state. continue to develop into new individuals. [0003] Embryo transfer in mammals began in the 1930s. Heape of the University of Cambridge in the United Kingdom first completed the embryo transfer test of immunity, and proposed the possibility that animal fertilized eggs can develop in the host mother. Subsequently, large animal embryo transfers were also successfully tested, such as: sheep (1934), goats (1974), pigs (195...

AI Technical Summary

Problems solved by technology

As for the embryo transfer of pigs, on the one hand, because pigs are multiple-birth animals, the embryo transfer has been caught in the misunderstanding of a narrow range of applications; Widely used like cattle and sheep

The method of pig embryo transfer is mainly surgical transplantation, but because the surgical embryo transfer requires strict surgical operations and causes certain damage to the recipient, its application in commercial production is limited to a certain extent

[0004] Moreover, pig genitals have complex structures, such as more and deeper cervical folds and longer uterine horns, etc., and there is no efficient non-surgical transplantation method like cattle. Surgical operations must be used for embryo collection and transplantation, which increases the difficulty and cost. , and adhesions often occur after surgery, resulting in that the same pig cannot be used repeatedly