Method for pig in-vitro fertilization and embryo transplantation

An in vitro fertilization and embryo transfer technology, applied in the field of pig in vitro fertilization and embryo transfer, can solve the problems of uterine horn length, receptor injury, adhesion, etc., and achieve the effect of high success rate, low cost and simple operation.

Inactive Publication Date: 2010-07-21
WENS FOOD GRP CO LTD +1
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the embryo transfer of pigs, on the one hand, because pigs are multiple-birth animals, the embryo transfer has been caught in the misunderstanding of a narrow range of applications; Widely used like cattle and sheep
The method of pig embryo transfer is mainly surgical transplantation, but because the surgical embryo transfer requires strict surgical operations and causes certain damage to the recipient, its application in commercial production is limited to a certain extent
[0004] Moreover, pig genitals have complex structures, such as more and deeper cervical folds and longer uterine horns, etc., and there is no efficient non-surgical transplantation method like cattle. Surgical operations must be used for embryo collection and transplantation, which increases the difficulty and cost. , and adhesions often occur after surgery, resulting in that the same pig cannot be used repeatedly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be described in detail below in conjunction with specific embodiments, where the exemplary embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0020] The method for pig in vitro fertilization and embryo transfer provided by the present embodiment comprises the following steps:

[0021] 1. In vitro embryo preparation

[0022] 1) Preparation of oocytes

[0023] Collect pig ovaries from the slaughterhouse and immediately put them in 39°C physiological saline with added antibiotics. After the pig ovaries are transported back to the laboratory, cut off the connective tissue on the surface of the ovaries and rinse 3 times with 39°C physiological saline (with antibiotics added) Finally, use a syringe to extract follicles with a diameter of 2-6mm, use a self-made ovum picking needle to select cumulus-oocyte complexes (COCs) with more than 3 layers of c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for pig in-vitro fertilization and embryo transplantation, which comprises the following steps: oocyte preparation; sperm capacitation; adjustment of sperm concentration through adding sperm receiving fluid and capacitation through placing the adjusted mixture into an incubator; in-vitro fertilization and in-vitro oosperm culture; and embryo transplantation which adopts an embryo transplantation tube for transplantation. The method for pig in-vitro fertilization and embryo transplantation provided by the invention can be operated simply, the in-vitro fertilization and embryo transplantation can be realized without surgery, no damage can be caused to the pig, the success rate is high, the cost is low, and the method has great significance for promotion of pig in-vitro fertilization, pig cloning and transgenic embryo research and production.

Description

technical field [0001] The invention relates to a method for artificial reproduction of animals, in particular to a pig in vitro fertilization method and an embryo transplantation method. Background technique [0002] Animal embryo transplantation is also called fertilized egg transplantation or gestation through the abdomen. It refers to taking out the early embryos after mating of a fine-bred female animal, or transplanting embryos obtained by in vitro fertilization or other methods into the uterus of another female animal with the same physiological state. continue to develop into new individuals. [0003] Embryo transfer in mammals began in the 1930s. Heape of the University of Cambridge in the United Kingdom first completed the embryo transfer test of immunity, and proposed the possibility that animal fertilized eggs can develop in the host mother. Subsequently, large animal embryo transfers were also successfully tested, such as: sheep (1934), goats (1974), pigs (195...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61D19/04
Inventor 吴珍芳赵云翔石俊松莫啟杯周荣周海斌刘德武
Owner WENS FOOD GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap