18 results about "Embryo cell" patented technology

This invention relates to the field of embryo resuscitation technology and discloses an auxiliary warming device for frozen embryo resuscitation, including a collection plate. A fixing ring is fixedly connected to the side wall of the collection plate, and a forward threaded groove is formed on the side wall of the fixing ring. The cover plate drives the separator ring to rotate clockwise through the contact rod and the fixing plate. Since the reverse threaded groove rotates in the opposite direction to the forward threaded groove, the rotating ring will move upward at this time. The upward rotating ring will drive the separator ring to move upward synchronously, so that the through hole gap coincides with the flow hole. At this time, the new solution on the outer wall of the separator ring flows downward through the flow hole and the through hole gap. Finally, the new solution mixes with the solution inside the collection plate. At this time, the pressure of the dripping will be absorbed by the top of the fixing ring and will not directly contact the solution inside the collection plate. When the operator twists the cover plate to close it, the flow hole and the through hole gap will coincide, and the new solution will mix with the solution inside the collection plate, effectively reducing the damage to the outer wall of the embryo cell membrane when injecting the new solution.

The invention provides genetically modified non-human animals that express chimeric human / non-human MHC I polypeptide and / or human or humanized β2 microglobulin polypeptide, as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified animals and methods of making the same. Methods of using the genetically modified animals to study various aspects of human immune system are provided.

The present application relates to a primer combination for amplifying whole genome DNA of sheep embryo cells and application thereof, and the primer combination is composed of 20 or 30 primers. The present application designs random primers based on sheep reference genome sequence, optimizes the reaction system based on MDA whole genome amplification technology, so that the whole genome DNA amplification product with high coverage and low mismatch rate can be generated by using 10 sheep embryo cells or as low as pg level genomic DNA, which can be directly adapted to downstream applications such as second-generation sequencing and targeted capture sequencing, and the detection cost is reduced.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

The application discloses an electroporation transfection protection fluid suitable for abalone embryo cell gene editing and application thereof, and belongs to the technical field of gene editing. The electroporation transfection protection fluid comprises a basic fluid and a cell protection agent; the basic fluid comprises NaCl, MgSO4, CaCl2, KCl, NaHCO3, NaBr, K2HPO4, glucose and glutathione; and the cell protection agent comprises polyethylene glycol 400. The electroporation transfection protection fluid can significantly reduce the deformity rate and mortality rate of abalone cells after electroporation technology, improve the gene transfection and gene editing efficiency, and reduce the cost of gene editing experiments, thereby laying a foundation for accelerating marine biological research and promoting environmental protection, species conservation and aquaculture.

This invention discloses a kit for amplifying the whole genome of bovine embryonic cells and its applications. The invention provides a primer set for amplifying the whole genome of bovine embryonic cells, consisting of 20 primers, each 6 nt in length. This invention can utilize 4-8 bovine embryonic cells or genomic DNA down to the pg level to amplify and generate whole-genome DNA amplification products with high coverage and low mismatch rate. These products can be directly adapted to downstream applications such as next-generation sequencing and targeted capture sequencing, while reducing detection costs. Furthermore, the three-step operation process of this invention (cell lysis-lysis termination-isothermal amplification) can complete efficient amplification within 3.5 hours.

The utility model relates to the field of assisted reproductive technology equipment discloses a kind of bovine in vitro embryo culture environment simulation device, including device body assembly, the device body assembly includes simulation device body, the outer surface of the simulation device body is rotatably connected with turnover observation window, the bottom of the simulation device body is fixedly connected with buffer rod by bottom mounting seat.The utility model is through the series connection effect of first spring damper, buffer spring and second spring damper, device forms gradient shock-absorbing network in three-dimensional space, horizontal direction impact is preferentially absorbed by first spring damper and converted into heat energy dissipation, vertical load is guided to spiral spring by buffer rod and carried out flexible support, extend the action cycle to reduce peak acceleration, moving block linkage mechanism cooperates second damper to realize the vector decomposition of oblique disturbance, finally reduce the vibration amplitude at culture dish, provide nearly stationary growth interface for embryo cell division.

To provide a method for producing a population of high titer recombinant adeno-associated virus (AAV) lacking prokaryotic sequences.SOLUTION: Culturing a human embryo cell strain in suspension, transfecting the human embryo cell strain with (a) nucleic acids sequences encoding helper proteins sufficient for rAAV replication, (b) nucleic acids sequences encoding AAVrep and AAVcap genes, and (c) a closed ended linear duplex rAAV vector nucleic acids comprising at least one inverted terminal repeats (ITR) sequence and a heterologous transgene operably linked to one or more regulatory elements, incubating the transfected human cell strain for about 40 to 400 hours, and optionally, B. lysing the transfected human cell line to purify the nucleic acid sequence encoding the rAAV, thereby producing the rAAV.SELECTED DRAWING: None

The invention discloses a method and a kit for whole genome sequencing and library building of embryo trace cells, and belongs to the technical field of molecular biology. The method comprises the following steps: (1) splitting 5-16 trace embryonic cells by using a cell splitting reagent, and releasing genome DNA (Deoxyribose Nucleic Acid); (2) carrying out enzyme digestion breaking on the genome DNA to obtain a DNA fragment; (3) connecting an amplification linker sequence to two ends of the DNA fragment; (4) carrying out PCR (Polymerase Chain Reaction) amplification by utilizing a fixed primer complementary with the amplification linker sequence, and constructing a sequencing library; wherein the step (1) to the step (4) are completed in the same reaction tube. Compared with a traditional single cell amplification method, the method is shorter in time consumption, simpler and more convenient to operate and lower in cost, and is helpful for quickly promoting the early screening of human individual genetic diseases and the early selection process in livestock and poultry breeding.

The invention provides a genetically modified non-human animal that comprises in its genome unrearranged T cell receptor variable gene loci, as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified non-human animal and methods of making the same. Various methods of using the genetically modified non-human animal are also provided.

The invention relates to a method for isolating embryonic stem cells (100) from at least one avian embryonic cell, comprising: isolating at least one embryo (101) around the developmental stage of oviposition; suspending embryonic cells (102) obtained from the embryo by dissociation step a) in an animal serum-free base medium supplemented with a combination of growth factors, at least one inhibitor and an animal serum substitute; seeding the embryonic cell suspension (103) on a feeder cell layer; culturing (104) the embryonic cells at least once passaged.

The utility model discloses a cow embryo three-dimensional culture system assisted by a degradable biological scaffold, which is characterized by comprising a culture main body, a micro-fluidic chip, a hollow fiber tube and a gas adjusting device, the culture main body comprises an inlet, an outlet, a culture cavity, a liquid inlet channel and a liquid outlet channel; the micro-fluidic chip, the hollow fiber tube and the culture main body are sequentially connected in the liquid flowing direction, and the gas adjusting device is connected to the hollow fiber tube. According to the culture system provided by the invention, the internal three-dimensional environment can be simulated through the degradable bracket and the gas regulation and control device, so that the normal growth and development of embryonic cells are facilitated, and the blastocyst rate of transforming the cells into blastocysts is increased.

The invention provides a genetically modified non-human animal that comprises in its genome unrearranged T cell receptor variable gene loci, as well as embryos, cells, and tissues comprising the same. Also provided are constructs for making said genetically modified non-human animal and methods of making the same. Various methods of using the genetically modified non-human animal are also provided.

The utility model relates to an embryonic cell operation mouth suction tube which comprises a mouth suction section at the front end, a connecting section at the middle end and a suction / discharge section at the rear end, the mouth suction section comprises a straight round tube, an inclined tube and a filter, one end of the straight round tube is connected with one end of the filter, and the other end of the straight round tube is connected with one end of the inclined tube; the connecting section comprises a hose, one end of the hose is connected with the other end of the filter, the suction / discharge section comprises a hard connecting pipe, one end of the hard connecting pipe is connected with the other end of the hose, and the other end of the hard connecting pipe is connected with a glass needle. According to the embryonic cell operation mouth suction tube provided by the utility model, scientific researchers can carry out various cell operations by using the mouth suction device under the condition that a mask is not taken off, so that the high maintenance of an experimental environment, the efficient achievement of an experimental purpose and the guarantee of the body safety of the scientific researchers are realized, and finally, the safe operation of a laboratory is guaranteed.

The application relates to the technical field of embryo engineering, and discloses a combined activation device for early embryos, which comprises a culture dish used for storing early embryos, a cell holding mechanism used for adjusting the pose of the early embryos and fixing the early embryos, and a combined activation mechanism comprising a capillary micro-needle and a first needle holder, wherein the capillary micro-needle comprises a liquid metal channel and a chemical substance channel; liquid metal and a wire arranged in the liquid metal channel form a liquid metal electrode used for electrically activating the early embryos; and the chemical substance channel is used for releasing chemical substances to chemically activate the early embryos. The combined activation mechanism integrates the functions of electrical activation and chemical activation, improves the convenience of combined activation of the early embryos, the capillary micro-needle can realize precise combined activation of local parts, such as nuclear material regions, of a single early embryo, reduces excessive electrical damage or chemical damage to other parts of the early embryo, and thus improves the activation rate of the early embryos.

A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available, are disclosed. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects. In accordance with one embodiment of the invention, incomplete genetic data is acquired from embryonic cells, fetal cells, or cell-free fetal DNA isolated from the mother's blood, and the incomplete genetic data is reconstructed using the more complete genetic data from a larger sample diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and / or genetic data taken from other related individuals.

This invention discloses a method and kit for constructing a library for whole-genome sequencing of a small number of embryonic cells, belonging to the field of molecular biology technology. The method includes the following steps: (1) lysing 5-16 small embryonic cells with a cell lysis reagent to release genomic DNA; (2) digesting the genomic DNA with enzymes to obtain DNA fragments; (3) ligating amplification adapter sequences to both ends of the DNA fragments; (4) performing PCR amplification using fixed primers complementary to the amplification adapter sequences to construct a sequencing library; wherein steps (1) to (4) are completed in the same reaction tube. Compared with traditional single-cell amplification methods, this method is faster, simpler to operate, and lower in cost, which helps to rapidly advance the early screening of human individual genetic diseases and the early selection process in livestock and poultry breeding.

The present disclosure relates generally to microfluidic systems for isolating embryonic cells from donor embryos, and methods of multiplying donor embryos comprising use of such systems. The present disclosure also relates to the use of such methods in animal breeding.