1 results about "Second polar body" patented technology

This invention provides a method for breeding large yellow croaker that is entirely female and triploid. First, pseudo-male large yellow croaker germplasm is obtained using sex reversal technology. Then, pseudo-males are identified using molecular markers for sex determination. These pseudo-males are mated with normal females to obtain wholly female fertilized eggs. Hydrostatic pressure treatment is then used to inhibit the expulsion of the second polar body from these fertilized eggs, inducing the formation of wholly female triploid large yellow croaker. Simultaneously, the concentration of the sex reversal reagent, the usage cycle, water temperature, accumulated temperature, pressurization time, pressure value, and hydrostatic pressure treatment duration were all optimized to achieve a 100% triploid rate and a 100% female rate, meeting the requirements for large-scale, industrialized breeding.