31 results about "Second polar body" patented technology

The invention discloses a gynogenesis method of grass carp and application thereof. The method comprises the following steps: taking the diploid sperms of a properly inactivated allotetraploid crucian carp as a stimulus to stimulate mature eggs of the grass carp, after activating for 2-3 min or fertilizing for 20-22 min, carrying out the water-cooling shock treatment for 10-12 min at 4-6 DEG C, and hatching and controlling the temperature by to obtain the gynogenesis grass carp restraining the discharge of a second polar body or the first cleavage. The method greatly improves the hatching rate and the survival rate of the gynogenesis diploid grass carp, simplifies the preparation process, and can prepare a gynogenesis grass carp colony with good characters and stable heredity.

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

The invention provides a method for culturing a Crassostrea angulata tetraploid. The method comprises the steps of firstly culturing a pacific oyster tetraploid, hybridizing the pacific oyster tetraploid with Crassostrea angulata so as to culture a hybridized triploid, fertilizing the hybridized Crassostrea angulata triploid with a Crassostrea angulata diploid sperm, carrying out anesthetization by virtue of magnesium chloride mixed liquid to inhibit the release of a second polar body so as to generate the tetraploid, wherein an inductive agent is safe and toxic-free. According to the method,the long induction process of the Crassostrea angulata triploid is solved, the survivable and stable tetraploid Crassostrea angulata can be cultured by virtue of the technical routes of hybridization,high permeation, anesthetization, molecular marking and the like, and the foundation is laid for the large-scale seedling growing of triploid Crassostrea angulata.

The invention discloses a method for inducing the gynogenetic development of Cichlid carp, which comprises the following steps: (1) collecting carp semen; (2) carrying out ultraviolet genetic inactivation treatment on carp semen; Carp semen after ultraviolet genetic inactivation treatment was used for dry insemination; (4) Fertilized eggs were treated with cold shock and placed in hatching containers for hatching. Normal surviving fry were gynogenetic fish. The present invention adopts the ultraviolet genetic inactivation sperm of common carp to stimulate the eggs of the cymbidium, inhibits the release of the second polar body of the eggs by cold shock, and promotes chromosome doubling, and successfully obtains the gynogenetic individuals of the cymbidium. Since the normal surviving individuals are gynogenetic fish, there is no need for subsequent identification and analysis of chromosomes and genetic markers, which significantly simplifies the identification procedure.

The invention relates to a physical method of polyploidy-breeding by introducing allotriploid scallops, put female scallops in filter seawater at the temperature of 16íµ after being dried in shade for 40 minutes to stimulate ovulation; put male scallops in filter seawater at the temperature of 16íµ after being dried in shade for 1 hour to stimulate sperm-drain, and then put said ovums and sperms produced in filter seawater at the temperature of 20íµ for fertilization following the mixing rate of 1í†5. When 30% of ovums have been drained out of second polar bodies after fertilization, force pressure of 20-30 MP for 20-10 minutes, hatch fertilized ovums according to the conventional method and get triploid scallops. The scallops cultured in said invention are characterized on fast,growth, high infertility and fresh texture, could make up for the shortages of low production and induced texture during the multiplication periods caused by diseases and high-density culturing in recent years.

The invention relates to chromosome manipulation technology, in particular to a method for continuous batch induction of Atlantic salmon triploid. Atlantic salmon sperm eggs are collected for artificial insemination, and the fertilized eggs are divided into 2-3 groups, and each group is successively subjected to hydrostatic pressure induction treatment to inhibit the discharge of its second polar body, thereby obtaining Atlantic salmon triploid; Pressure induction is, after fertilization 300-400 degrees (temperature ℃ × time min), the fertilized eggs of the first group are subjected to hydrostatic pressure treatment to obtain triploid Atlantic salmon; after the fertilized eggs of the first group are taken out, immediately The second group of fertilized eggs was induced by hydrostatic pressure according to the above process, so that the same batch of fertilized eggs could be continuously induced and treated. By adopting the method of the invention, 100% Atlantic salmon triploids can be continuously and efficiently induced.

The invention provides a cultivation method for carassius auratus gynogenesis fries. The method comprises the steps of selecting sexually mature diploid carassius auratus parents and collecting sperm and ovums; performing ultraviolet irradiation on Hanks liquid-diluted sperms to inactivate genetic materials thereof; performing artificial insemination on the inactivated sperms and the ovums; restraining the discharge of second polar bodies of meiosis by using a cold shock method to double genomes of the ovums and thus to cultivate viable carassius auratus gynogenesis diploid fries. Microsatellite marker identification proves that the genetic materials of all the gynogenesis fries are from the female parent; morphological measurement indicates part of the gynogenesis fries has obvious early growth dominance. The method has the advantages of simple operation, high efficiency, stability and high practicality.