2 results about "Second polar body" patented technology

The invention discloses a method for efficiently producing triploid oysters, and belongs to the technical field of aquaculture breeding. The method comprises the following steps: S1, obtaining sperms and ova of diploid oysters, and carrying out sperm-ova mixed fertilization; s2, performing induction treatment on the fertilized eggs to inhibit release of a second polar body; s3, collecting fertilized eggs subjected to induction treatment, cleaning, and hatching to a larva stage; s4, 24-72 hours after fertilization, screening based on larva individual size differences, and collecting a target larva population; and S5, culturing the larvae screened in the step S4. According to the method provided by the invention, even if the initial induction rate is only about 70%, the larva population with the triploid rate higher than 90% can be stably obtained finally, the hatching rate and the survival rate are high, the method is easy and convenient to operate, and the requirement for induction conditions is low; the method is suitable for triploid preparation of precious oyster varieties in which a large number of fertilized eggs are difficult to obtain under strong induction conditions or oyster strains with important economic characters.

This invention provides a method for breeding large yellow croaker that is entirely female and triploid. First, pseudo-male large yellow croaker germplasm is obtained using sex reversal technology. Then, pseudo-males are identified using molecular markers for sex determination. These pseudo-males are mated with normal females to obtain wholly female fertilized eggs. Hydrostatic pressure treatment is then used to inhibit the expulsion of the second polar body from these fertilized eggs, inducing the formation of wholly female triploid large yellow croaker. Simultaneously, the concentration of the sex reversal reagent, the usage cycle, water temperature, accumulated temperature, pressurization time, pressure value, and hydrostatic pressure treatment duration were all optimized to achieve a 100% triploid rate and a 100% female rate, meeting the requirements for large-scale, industrialized breeding.