24 results about "Stem cell line" patented technology

The invention provides a human-derived recessive retinal degeneration disease organ-like model based on Prom1 gene knockout. Specifically, the invention provides a specific gRNA targeting a Prom1 gene exon 9, and the specific gRNA can be used for efficiently knocking out the Prom1 gene of the human embryonic stem cell. The invention also provides a Prom1 gene knockout stem cell line derived from the human embryo pluripotent stem cell H9 and a retinal organ disease model. The retina-like organ can provide a research model for clinical treatment drugs.

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.

This invention belongs to the medical field and provides a human trophoblastic stem cell line derived from complete hydatidiform mole and its applications. The human trophoblastic stem cell line, with accession number CGMCC No. C2024411, can be induced to differentiate into syncytiotrophoblast and extravillous trophoblast, mimicking the differentiation and developmental defects of the trophoblastic lineage in hydatidiform mole. It can be used as an in vitro research model for complete hydatidiform mole and has broad application prospects in drug target research and genetic studies.

The application discloses a method for stably producing exosomes of regenerative repair and anti-inflammatory molecules of sheep umbilical cord mesenchymal stem cells and application thereof in skin care products, and comprises the following steps: S1, using healthy sheep umbilical cord as raw material, obtaining primary sheep umbilical cord mesenchymal stem cells through aseptic treatment, Wharton's jelly extraction and tissue block adherent culture; S2, introducing an immortalization gene into the primary sheep umbilical cord mesenchymal stem cells through a lentivirus transduction technology, and obtaining an immortalized sheep umbilical cord mesenchymal stem cell line through resistance screening; S3, collecting supernatant after amplification culture of the immortalized sheep umbilical cord mesenchymal stem cell line, and purifying through multi-stage centrifugal impurity removal and PEG precipitation method to obtain stably produced exosomes containing regenerative repair and anti-inflammatory molecules. The exosomes containing regenerative repair and anti-inflammatory molecules provided by the application exhibit significant advantages in yield and quality, and have outstanding application value in the fields of medical beauty anti-aging and skin care products.

The invention provides an immortalized mesenchymal stem cell line capable of simultaneously expressing IL1-RN and COL17AD protein active segments as well as a preparation method and application of the immortalized mesenchymal stem cell line, and belongs to the technical field of genetic engineering. The method specifically comprises the following steps: (1) constructing a pMSCV-BMI-P2A-TERT virus; (2) infecting the mesenchymal stem cells, and performing passage to obtain immortalized mesenchymal stem cells; (3) constructing a pMSCV-IL1-RN-P2A-COL17AD virus (pMSCV-IL1-RN-P2A-COL17AD And (4) infecting the immortalized mesenchymal stem cells by using the pMSCV-IL1-RN-P2A-COL17AD virus obtained in the step (3), and carrying out passage, so as to obtain the immortalized mesenchymal stem cell line capable of simultaneously expressing IL1-RN and COL17AD protein active segments. The cell supernatant of the cell line provided by the invention has the function of promoting skin injury repair, and can be applied to functional cosmetics and skin repair drugs.

The invention belongs to the field of medicine, and provides a complete grape embryo-derived human trophoblast stem cell line and application thereof. The preservation number of the human trophoblast stem cell line is CGMCC No.C2024411, and the human trophoblast stem cell line can be induced and differentiated into a syncytial trophoblast and an extravillus trophoblast, can simulate differentiation and development defects of a grape embryo trophoblast pedigree, can be applied as an in-vitro research model of a complete grape embryo, and has a wide application prospect in drug target research and genetic research.

The invention belongs to the technical field of cell culture, and particularly relates to a construction method of an immortalized goat umbilical cord mesenchymal stem cell line. The method comprises the following steps: by taking newborn goat lamb umbilical cord tissues as a research object, cleaning goat lamb umbilical cords, stripping blood vessels, separating Wharton's jelly, cutting the Wharton's jelly into 1mm < 3 > tissues, culturing by using a DMEM / F12 complete culture medium containing fetal calf serum, carrying out passage, cryopreservation, repeated resuspension and centrifugation, and inoculating into a culture dish for culture; lentiviruses containing SV40 large T antigens are packaged in vitro and are transduced into goat umbilical cord mesenchymal stem cells, and immortalization is realized after long-term subculture. The result of the embodiment shows that the construction method of the immortalized goat umbilical cord mesenchymal stem cell line provided by the invention is strong in repeatability, simple and efficient in operation steps, good in stem cell growth state and stable in physiological state.

The application discloses a kind of construction methods and applications of motor neuron MNX1 reporter cell lines, belong to gene editing and stem cell technical field.The application develops a kind of expression motor neuron MNX1 reporter gene vector system by screening high-activity sgRNA, and vector system includes the guide vector of single-stranded guide RNA targeted to the vicinity of the termination codon of human motor neuron MNX1 gene, and the donor vector carrying the homologous arm in the vicinity of the termination codon of human motor neuron MNX1 gene.The application also includes the construction method of MNX1 reporter gene human pluripotent stem cell line into the above-mentioned vector system, the application can be applied to identify stem cell differentiation source human motor neuron, and the morphology and function of motor neuron are researched under the condition of living cell, provide important platform for the pathogenesis research of muscle-related disease, treatment method evaluation and new treatment target exploration.

The present invention relates to a ground extracellular matrix (ECM) produced by a human stem cell line with chondrogenic activity, wherein the human stem cell line is genetically modified to overexpress Bone Morphogenetic Protein-2 (BMP-2). The invention further relates to a composition comprising said extracellular matrix, and a gel-like substrate or precursor thereof. The invention further relates to a kit comprising said composition. The invention further relates to a method of manufacturing said composition. The invention further relates to a method for bone and / or bone marrow formation in vivo. The invention further relates to a method for testing a candidate pharmaceutical product. The invention further relates to a method for identifying treatment responders. The invention further relates to said composition for use in medicine.

The invention discloses an establishment method of induced pluripotent stem cells capable of overcoming immunogenicity and derived mesenchymal stem cells of the induced pluripotent stem cells, belongs to the technical field of biology, and solves the problems of limited passage times, large individual difference and immunological rejection of the mesenchymal stem cells in the prior art. The method comprises the following steps: reprogramming starting somatic cells to establish an induced pluripotent stem cell line, and continuously picking monoclonal passage to obtain a monoclonal induced pluripotent stem cell line; and performing beta2-microglobulin knockout on the monoclonal induced pluripotent stem cell strain to obtain beta2-microglobulin knockout induced pluripotent stem cell monoclone, namely the induced pluripotent stem cell capable of overcoming immunogenicity. The invention develops a method for inducing wild type and B2M knockout type hiPSC to differentiate into the iMSC by regulating an embryonic development signal channel to generate the iMSC, and the differentiated iMSC-WT and iMSC-KO are extremely similar to UCMSC in phenotype.

The invention relates to the technical field of biology, and particularly provides a glycyrrhiza glabra stem cell line and application thereof. The glycyrrhiza glabra stem cell line is preserved in the China General Microbiological Culture Collection Center, the preservation number of the glycyrrhiza glabra stem cell line is CGMCC No.46600, the glycyrrhiza glabra stem cell line is named as glycyrrhiza glabra stem cell line gc001, and the glycyrrhiza glabra stem cell line has high secondary metabolite content and can be directly used for high-end skin care products and medical products, and the glycyrrhiza glabra resource value is expanded.

The invention provides a method for promoting induction of human totipotent stem cells, belongs to the technical field of biology, and particularly relates to a method for promoting induction of the human totipotent stem cells to the totipotent stem cells through OTX2 knockout or knockdown, the method comprises the following steps: S1, constructing an OTX2 knockout or knockout plasmid vector; s2, establishing a human pluripotent stem cell line with OTX2 knocked down or knocked out; and S3, inducing the human pluripotent stem cells of which the OTX2 is knocked down or knocked out into totipotent stem cells, which are also called 8-cell-like cells (8CLCs). According to the invention, the efficiency of inducing the human pluripotent stem cells into the totipotent stem cells can be improved, and the application of the totipotent stem cells in in-vitro blastocyst-like induction, in-vivo chimeric embryo formation and the like is provided.

The present invention relates to a novel stem cell line derived from a Paralichthys olivaceus fillet and use thereof and, more specifically, to: a stem cell line (Accession No. KCLRF-BP-00545) derived from a Paralichthys olivaceus fillet; cultured meat comprising the stem cell line; and a food composition comprising the cultured meat. The stem cell line according to the present invention has pluripotency, thus enabling differentiation into various types of cells, exhibits an excellent growth rate even at low FBS concentrations, and is easily stored, and thus can be effectively used as a main raw material for the mass production of economical aquatic cell-cultured foods.

The invention discloses a healthy individual-derived autologous tumor stem cell line establishment method, which is characterized by comprising the following two stages: a first stage, inducing healthy human-derived somatic cells into hiPSC; in the second stage, the hiPSC is induced to be converted into the tumor stem cells, specifically, firstly, a tumor-derived conditioned culture medium is prepared, then under the synergistic effect of the tumor-derived conditioned culture medium and an induction adjuvant, the hiPSC is induced to be converted into the tumor stem cells, and the healthy individual-derived tumor stem cells are obtained after culture. According to the invention, the somatic cell-derived iPSC of healthy people can be induced into tumor stem cells, and the method can be well applied to cancer prevention, drug screening and health management.

The invention provides a preparation method of a low-immunogenicity induced pluripotent stem cell line and a method for preparing low-immunogenicity hematopoietic stem / progenitor cells from the cell line through induced differentiation, efficient differentiation from B2M gene knockout iPSC to CD34 + CD45 + hematopoietic stem / progenitor cells is realized, and a new stem cell source is provided for clinical research and application.

The present application relates to a method of deriving a human embryonic stem cell (hESC) line under feeder-free and heterologous conditions.

Embodiments of the present disclosure include compositions and methods related to engineered monoclonal TCR armed genetically engineered T (TARGET) cells for ready-to-use use for clinical therapy. In particular embodiments, the TARGET cells are produced from a pluripotent stem cell line and thus can be infinitely supplied. The TARGET cell products can be conventional alpha beta T cells, gamma delta T cells and unconventional T cells such as constant natural killer T (iNKT) cells, non-constant natural killer T (NKT) cells, mucosa related constant T (MAIT) cells and the like. The TARGET cell product can also be designed as a CD8-positive or CD4-positive T cell subtype, and the CD4-positive TARGET cell can be further designed as a variety of TH polarized subtypes.

Disclosed herein is a method of isolating a stem cell line derived from a tissue from a primary meristem in a leaf of a plant, including the steps of sterilizing at least a part of the leaf containing the central or other vein; cutting through the central or other vein of the leaf to expose primary meristematic cells from the central or other vein inner vascular tissue; and culturing cells from said exposed cells, which circumvents the difficulties of surface sterilization when other types of explants are used. Also disclosed herein are stem cell lines obtained using the method.

The invention discloses an immortalized bovine muscle-derived stem cell line as well as a construction method and application thereof, and belongs to the technical field of stem cells and biological cell lines. The bovine muscle-derived stem cell line is characterized in that telomerase reverse transcriptase (TERT) and cyclin dependent kinase (CDK) genes are overexpressed in a mode of lentivirus packaging and infection, a cell strain which is single in source and stably expresses a target gene is obtained through monoclonal screening, gene verification and functional verification, and the verification shows that the cell line overexpresses the CDK4 and TERT genes, so that the cell line can be used for preparing the bovine muscle-derived stem cell line with the stable expression of the target gene. The monoclonal cell strain can stably proliferate and maintain good differentiation characteristics. The immortalized bovine muscle-derived stem cell line is prepared, and a good seed cell resource is reserved for commercialized production of cultured meat.

A construction method and use of a gastruloid stem cell line. Gastruloid stem cells that can be passaged stably are established in this research. The cells maintain the pluripotency of stem cells to a certain extent, exhibit gene and protein expression of cells from three germ layers, endoderm, mesoderm, and ectoderm, during gastrulation, and have characteristics consistent with those of cells during gastrulation, so that key characteristics of cells during gastrulation can be well reproduced. A three-dimensional gastruloid model that can mimic gastrulation can be constructed in mice or in vitro by using the stem cell line, and key biological events can be partially reproduced, so that key characteristics of post-implantation embryos during gastrulation can be well reproduced. Through this model, an in vitro platform for screening drugs affecting early embryonic development can be established, providing a reference for clinical medication.

The invention relates to the technical field of biotechnology, and discloses a construction method of a bovine originating embryonic stem cell line, which comprises the following steps: (1) obtaining in vitro fertilized bovine early blastocyst; (2) trophoblast cells of the blastocyst are removed, an inner cell cluster is inoculated into a culture system containing a feeder layer, and the culture system is a basic culture medium added with FGF2 and WNT pathway inhibitors; and (3) culturing until an inner cell cluster forms a cell set drop, and carrying out digestion passage to obtain the stably proliferated bovine originated embryonic stem cell line. According to the construction method and the application of the bovine embryonic stem cell line, a specific initial induction combination of 'FGF2 + WNT inhibitor IWR1' is adopted, and a cell mass in an early blastocyst is used for establishing the line, so that the bovine embryonic stem cell line which is uniform in form, can be stably passaged for more than 26 generations and still keeps typical embryonic characteristics is successfully obtained; the problems of unstable state and easy differentiation of the bovine stem cell line in the prior art are solved.

The invention discloses a method for three-dimensional multiplication culture of a hepatic stem cell line. The method comprises the following steps: inoculating cells of the liver stem cell line into a low-adsorption culture plate containing a HepSC culture medium, and culturing at the rotating speed of 100-180 rpm to enable the cells to form cell spheres. According to the invention, the dependence of the HepSC cell line on mouse matrigel and adherent culture is effectively solved, a new three-dimensional culture way without matrigel is provided for large-scale amplification, and clinical transformation of the liver stem cell line can be promoted.

This invention provides a method for deriving pluripotent stem cell lines from mammals and livestock. [Solution] A method is provided comprising (a) culturing a mammalian livestock embryo that is at least 7 days and within 21 days after fertilization in ex vivo for a culture period of at least 4 days and within 21 days after fertilization to obtain an embryo containing epiblast cells and / or late pluripotent stem cells; (b) isolating epiblast cells and / or late pluripotent stem cells from the embryo; and (c) culturing the epiblast cells and / or late pluripotent stem cells under conditions suitable for the proliferation of undifferentiated mammalian livestock pluripotent stem cells to obtain a population of mammalian livestock pluripotent stem cells and deriving a mammalian livestock pluripotent stem cell line.

The present application relates to the technical field of biology, and particularly provides a licorice stem cell line and application thereof. The licorice stem cell line of the present application has been preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 46600, is named as licorice stem cell strain gc001, has a high content of secondary metabolites, and can be directly used in high-end skin care products and medical products, and expands the value of licorice resources.