The invention relates to the technical field of
organoid culture, in particular to a bone trabecula
organoid with
osteoclast metabolism activity as well as preparation and application of the bone trabecula
organoid. The method comprises the following steps: culturing
bone marrow primary cells, collecting mononuclear cells and a culture medium containing M-CSF, and performing adherence and amplification to obtain
osteoclast precursor cells; uniformly mixing the
osteoclast precursor cells with a bone organ with autonomous mineralization ability, adding the mixture into a low-adhesion plate, and culturing the mixture in a culture medium containing M-CSF to obtain a bone trabecula organ; and finally, carrying out
induction treatment on the bone trabecula-like organ by utilizing a culture medium containing
RANKL and M-CSF to obtain the bone trabecula-like organ with osteoclast
metabolism activity. The bone trabecula-like organ with osteoclast
metabolism activity prepared by the invention can be further applied to osteoclast-osteogenesis interaction research,
drug screening or target
verification.