35 results about "Primary bone" patented technology

Provided are methods for generating immune cells of the desired type and specificity in a host. The methods may be used to treat a disease or disorder, such as a tumor in a patient. Target cells, preferably hematopoietic stem cells such as primary bone marrow cells are transfected with a polynucleotide encoding a T cell receptor with the desired specificity. The transfected cells are then transferred to the host where they develop into mature, functional immune cells. The source of the T cell receptor can determine the stem cell's fate. Thus transfecting stem cells with TCRs from cytotoxic cells will lead to the generation of cytotoxic T cells in the host, while TCRs from helper cells will produce helper cells. Both arms of T cell immunity can be generated simultaneously in a host. Additionally, the immune response to the desired antigen can be further stimulated by immunizing the host with the antigen.

A clip to interconnect primary and secondary bone zones having edges and surfaces, comprising in combination a first tab to extend proximate a surface of the secondary bone zone; a second tab associated with the first tab, and located to extend proximate a surface of the primary bone zone, the second tab having at least one barb oriented to engage the primary bone to resist displacement of the second tab in a longitudinal direction toward the secondary bone zone.

A bone fixation device may include a primary bone plate. The primary bone plate may include an elongate member having a first fastener-receiving passageway therein to receive a first fastener to anchor the primary bone plate to a long bone of a patient, and an enlarged head member extending from the elongate member and having a secondary bone plate-receiving passageway therein. The bone fixation device may also include a secondary bone plate received in the secondary bone plate-receiving passageway and having a second fastener-receiving passageway therein to receive a second fastener to anchor the secondary bone plate to fractured end portions of the long bone of the patient.

A bone fixation device may include a primary bone plate. The primary bone plate may include an elongate member having a first fastener-receiving passageway therein to receive a first fastener to anchor the primary bone plate to a long bone of a patient, and an enlarged head member extending from the elongate member and having a secondary bone plate-receiving passageway therein. The bone fixation device may also include a secondary bone plate received in the secondary bone plate-receiving passageway and having a second fastener-receiving passageway therein to receive a second fastener to anchor the secondary bone plate to fractured end portions of the long bone of the patient.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and / or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

Bone anchor assemblies are disclosed herein that can provide for improved fixation as compared with traditional bone anchor assemblies. An exemplary assembly can include a bracket or wing that extends down from the receiver member and accommodates one or more auxiliary bone anchors that augment the fixation of the assembly's primary bone anchor. Another exemplary assembly can include a plate that is seated between the receiver member and the rod and accommodates one or more auxiliary bone anchors that augment the fixation of the assembly's primary bone anchor. Another exemplary assembly can include a hook that extends out from the receiver member to hook onto an anatomical structure or another implant to augment the fixation of the assembly's primary bone anchor. Surgical methods using the bone anchor assemblies described herein are also disclosed.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and / or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

The invention discloses a method for preparing a bone white soup basal material for hot pot. The preparation method comprises the following: a step of preparing: an animal bone is extracted to obtain primary bone soup, and the primary bone soup is added with ingredients and auxiliary agents, so that the protein content in weight percentage is between 10 and 50 percent, the fat content in weight percentage is between 5 and 35 percent, the salt content in weight percentage is between 12 and 18 percent, and the pH value is between 3.5 and 6.2; a step of sterilization: the primary bone soup is kept at a temperature of between 60 and 85 DEG C for 23 to 42 minutes: a step of homogenizing: the primary bone soup is homogenized under the working conditions the temperature is between 40 and 60 DEG C and the pressure is between 10 and 30MPa; and a step of drying: the air inlet temperature is between 190 and 220 DEG C, the air outlet temperature is between 80 and 110 DEG C, and the rotary speed of a centrifugal disc is between 13,000 and 15,600 rev / min. The bone white soup basal material prepared by the method is of a powder shape or a paste shape according to use requirement, and has the advantages of having flavor and taste of the corresponding animal and convenient use, and is used to prepare hot pot white soup bedding material, and ensures that the bone white soup basal material stably keeps milk white.

The invention discloses a yeast hollow beta-glucan shell wrapped GMP-BSA protein targeting macrophage delivery system. The system allows BSA to be coated in a yeast shell through using a compact layer formed through the electrostatic adsorption effect of chitosan (CS), tripolyphosphate (TPP), sodium alginate and BSA. GMP-BSA particles prepared in the invention have very good protein release behavior in in-vitro experiments, and can avoid protein loss. The particles highly specifically target the macrophages, such as Raw 264.7 cells, primary BMDM cells (primary bone marrow macrophages) and peritoneal macrophages (PEMs), and are not devoured by NIH3T3, AD293, HeLa, Caco-2 and other non-macrophage tumor cells, and neutral granulocytes in blood. The macrophages play a great role in the pathogenesis of various diseases, and are very important potential target spots. The macrophages are key antigen transfer cells, and are very important in vaccine design. The system can highly selectively deliver various proteins to the macrophages in a targeting manner, and also has very wide application prospect in the field of targeting drug administration and the field of macrophage vaccine design.

Bone anchor assemblies are disclosed herein that can provide for improved fixation as compared with traditional bone anchor assemblies. An exemplary assembly can include a bracket or wing that extends down from the receiver member. The distal portion of the wing can define a bone anchor opening through which one or more auxiliary bone anchors can be disposed to augment the fixation of the assembly's primary bone anchor. A distal surface of the distal portion of the wing can be obliquely angled relative to a proximal-distal axis of the spanning portion to face one of a caudal direction or a cephalad direction. A distal surface of the distal portion of the wing can be obliquely angled relative to the proximal-distal axis of the spanning portion to face one of a medial direction or a lateral direction. Surgical methods using the bone anchor assemblies described herein are also disclosed.

Bone anchor assemblies are disclosed herein that can provide for improved fixation as compared with traditional bone anchor assemblies. An exemplary assembly can include a bracket or wing that extends down from the receiver member. The distal portion of the wing can define a bone anchor opening through which one or more auxiliary bone anchors can be disposed to augment the fixation of the assembly's primary bone anchor. A distal surface of the distal portion of the wing can be obliquely angled relative to a proximal-distal axis of the spanning portion to face one of a caudal direction or a cephalad direction. A distal surface of the distal portion of the wing can be obliquely angled relative to the proximal-distal axis of the spanning portion to face one of a medial direction or a lateral direction. Surgical methods using the bone anchor assemblies described herein are also disclosed.

The invention provides a method for separating and culturing poultry bone mesenchymal stem cells by using a bone marrow tissue block, which comprises the following steps of: after obtaining primary bone mesenchymal stem cells by a bone marrow tissue block direct wall-adherence method, carrying out in-vitro subculturing, thereby obtaining the purified poultry bone mesenchymal stem cells. Compared with the traditional method, the method has the advantages of short separation time, large obtainment and higher purity of the cells, stronger in-vitro multiplication capacity of the cells and the like and can provide abundant material sources for research application and tissue engineering of the poultry bone mesenchymal stem cells.

The invention relates to the technical field of orthopedics and discloses an orthopedic implant. The orthopedic implant is spherical in shape and concave in a region of a bottom surface portion of theorthopedic implant to form an inner concave surface for contacting an acetabular liner or a femoral ball head to form a joint movable friction pair; at least a portion of that bottom region of the external spherical surface of the orthopedic implant extend outwardly to form an extension structure for contacting the human primary bone outside the acetabular fossa aft the orthopedic implant is implanted into the acetabular fossa of the human primary pelvis. In the manner described above, the present invention can improve the biological fixation performance of orthopedic implants.

Bone anchor assemblies and related methods are disclosed herein that can provide for improved fixation of a primary bone anchor. A bone anchor assembly can include a wing with a distal portion that can define a plurality of auxiliary bone anchor openings. Each auxiliary bone anchor opening can receive an auxiliary bone anchor that can augment fixation of a primary bone anchor of the bone anchor assembly. The plurality of auxiliary bone anchor openings can be oriented such that, when the wing is coupled to a primary bone anchor assembly of a vertebral level in a first configuration, at least one auxiliary bone anchor can be driven to extend across a facet plane of the vertebral level and, when coupled in a second configuration, each auxiliary bone anchor received within the wing can be driven to conform to the vertebral level.

The invention belongs to the technical field of regenerative medicine, and particularly relates to a sodium alginate / collagen composite bone scaffold as well as a preparation method and application thereof, and the preparation method comprises the following steps: providing hydroxyapatite hollow microspheres loaded with antibacterial drugs; carrying out a pre-crosslinking reaction on water, hydroxyapatite nanoparticles, a collagen crosslinking agent, collagen, D-glucolactone, sodium alginate and the hydroxyapatite hollow microspheres loaded with the antibacterial drugs, so as to obtain a pre-crosslinked mixture; printing the pre-crosslinked mixture to obtain a primary bone scaffold; subjecting the primary bone scaffold and bivalent copper ions to a re-crosslinking reaction so as to prepare the sodium alginate / collagen composite bone scaffold. The sodium alginate / collagen composite bone scaffold obtained by using the preparation method disclosed by the invention has good mechanical properties and a relatively stable structure, and has an antibacterial effect.

The invention discloses a megalobrama amblycephala bone tissue culture method. The method includes steps: 1), selecting megalobrama amblycephala of 3 months old as an experimental material; 2), taking caudal vertebra, costa and its connective tissue around of megalobrama amblycephala; 3), flushing with PBS, wetting a tissue block in fetal calf serum, and using forceps to put the tissue block at the bottom of a culture bottle; 4), putting the culture bottle into a culture box for inverted culture; 5), daily observing cell growth and replacing a culture medium; 6), performing trypsin digestion passage after cell convergence degree reaches 80% to obtain megalobrama amblycephala primary bone tissue cells; 7), using trypsin for digestion passage of the cells when convergence degree of the megalobrama amblycephala primary bone tissue cells reaches 70-80%, centrifuging before re-suspending the cells, and inoculating the cells into two new culture bottles to continue culture to obtain passage cells. The method is easy to implement and simple and convenient to operate, the problems of limitation on sample individual size and limitation on using of a trypsin digestion method are solved, and a base material is provided for related study on fish skeleton growth and development and the like in the future.

The invention discloses a design method and device for a bone cement spacer female mould. The design method for the bone cement spacer female mould includes: establishing a primary three-dimensional CAD model of a joint prosthesis according to acquired point cloud data of the joint prosthesis of a patient; obtaining a three-dimensional CAD model of the joint prosthesis according to the measured actual size of a joint of the patient; defining a joint surface of a relevant die of the three-dimensional CAD model of the joint prosthesis according to analysis on a connection manner and a working process of the three-dimensional CAD model of the joint prosthesis, and establishing a primary bone cement spacer female mould; and editing the primary bone cement spacer female mould to obtain the bone cement spacer female mould according to the characteristics of bone cement. The design method and device can quickly produce a high-precision high-matching-degree bone cement spacer female mould.

The invention belongs to the technical field of artificial bones, in particular to a hip joint prosthesis. The hip joint prosthesis comprises a thighbone handle, a ball head, an inner lining, an acetabular cup and connection screws, wherein the thighbone handle is inserted into bone marrow of a thighbone at a location wherein transplanting needs to be performed; the acetabular cup is connected with a fossa acetabuli where transplanting needs to be performed through the connection screws; the thighbone handle is connected with the acetabular cup through the ball head and the inner lining; and the curvature of the inner side of the thighbone handle is 127-135 degrees. Through the adoption of the technical scheme of the hip joint prosthesis provided by the invention, through cooperation of each component of the hip joint prosthesis, the transplanted hip joint prosthesis is stably connected with the primary bone; besides, the curvature of the inner side of the thighbone handle is set to be127-135 degrees, so that the thighbone handle can be attached to the bone marrow of the thighbone of the location where transplanting needs to be performed, fixing is convenient, and after fixing, loosing is not liable to generate; and the technical defect that in the prior art, the transplanted hip joint prosthesis and primary bone locations are easy to loosen, and periodic replacement is required is solved.

New compounds and pharmaceutical compositions comprising active ingredients having inhibition effects on osteoclast differentiation are provided. The pharmaceutical compositions comprising the new compounds can be used as medicines for treating metabolic bone diseases such as bone metastatic cancer, solid cancer bone metastasis, musculoskeletal complication by solid cancer bone metastasis, hypercalcemia by malignant tumor, multiple myeloma, primary bone tumor, osteoporosis, rheumatoid arthritis, osteoarthritis, periodontal disease, inflammatory resorption of alveolar bone, inflammatory resorption of bone, and Paget's disease.

The invention belongs to the field of biotechnology, and relates to a fluorescent protein labeling method for studying osteoclast fusion, which is characterized in that: primary mouse bone marrow mononuclear cells (bone marrow monocytes, BMMs) containing CTSK-Cre transgene Mix culture with RosamTmG-containing transgenic mouse primary bone marrow mononuclear cells at a ratio of 3:1-1:3, perform osteoclast differentiation induction and fluorescence microscope observation steps, the osteoclast fusion process can be visually and visually observed, thereby Observe the morphology of osteoclasts, the number of nuclei and the number of fused cells that can be quantitatively studied.

The present invention relates to the use of the protein-polymer conjugate described in the specification to treat various diseases, including primary myelofibrosis, polycythemia vera and essential thrombocythemia.

The present invention relates to the synthesis of porous polymer scaffold from polyethyleneglycol-polyurethane having castor oil linkages under controlled conditions and their use as stem cell delivery vehicles thereby accelerating the tissue regeneration process. The present invention further studies the biodegradability, stability and biocompatibility of porous polymer scaffolds in varios cell lines and primary bone marrow stem cells. Particularly the present invention further relates to the physio-chemical characterization of the porous polymer scaffolds.

A retro guidewire reamer includes a cutting member, and a mechanism for moving the cutting member from a closed position to a deployed position in a single manual motion. Once a desired size of a bone tunnel is established, a surgeon uses the reamer to create a primary bone tunnel over a guidewire from the outside in. The surgeon retracts the guidewire, and activates the mechanism to deploy the cutting member within the bone joint to conform to the size of a tendon graft. The surgeon uses the deployed cutting member to create a counter bore through the bone in a retrograde manner. Once the counter bore is drilled, the surgeon activates the mechanism to close the cutting member, allowing the reamer to be withdrawn through the primary tunnel. The retro guidewire reamer can be used to provide more accurate bone tunnel placement during ligament reconstruction surgery.

The invention provides a method and / or composition for the treatment of bone cancer including primary bone cancer and secondary bone cancer, breast cancer, skin cancer, nasopharyngeal carcinoma, oral cancer, vulva cancer, prostate cancer, cervical cancer, melanoma including melanocarcinoma by percutaneous and / or transmucosal administration of the interferon. Further, the invention provides a method and / or composition for the treatment of skin, subcutaneous, mucosal and / or submucosal primary cancer and cancer metastatic lesions by percutaneous and / or transmucosal administration of the interferon, especially a method and / or composition for the treatment of bone cancer pain including pain resulted by secondary bone cancer.

The invention relates to natural biomineralization powder. An adopted technical scheme is that a preparation method of the biomineralization powder comprises the following steps: cleaning animal bones, removing impurities from the surface of the bones and grease and colloid from primary bones, carrying out constant temperature calcination on the processed bones in a muffle furnace at 800DEG C for6-8h, naturally cooling, and crushing. The biomineralization powder which is prepared according to artificially and chemically synthesized hydroxyapatite in the invention has the advantages of simplemethod and wide material source. The biomineralization powder contains trace elements which are not possessed by the artificially and chemically synthesized hydroxyapatite and are possessed by human bones, and the ratio of the trace elements completely accords with organisms, so a wide prospect is opened for the biomineralization powder utilization.

The invention discloses a human primary myelofibrosis cell line and its construction method and application. The primary myelofibrosis cell line is named human primary myelofibrosis cell line ZYXY‑M2, and was deposited in the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on January 20, 2021, with the preservation number CCTCC NO :C202145. The present invention is obtained by extracting and separating mononuclear cells from the peripheral blood of patients with clinical primary myelofibrosis, and culturing them in vitro for continuous natural passage. The leukemia cell line is negative for JAK2, CALR, and MPL mutations, positive for ASXL1 mutations, positive for TP53 mutations, positive for IKZF1 mutations, positive for IDH1 mutations, positive for FLT3 mutations, and positive for TET1 mutations. With good in vitro proliferation ability, it can be used as a cell material for studying the mechanism of human primary myelofibrosis and individualized treatment in vitro. It can also be used for in vivo and in vitro research of drug screening, evaluation, and guidance for human primary myelofibrosis. Clinical medication.

The invention belongs to the technical field of regenerative medicine, and in particular relates to a sodium alginate / collagen composite bone support and a preparation method and application thereof, comprising the following steps: providing hydroxyapatite hollow microspheres loaded with antibacterial drugs; Limestone nanoparticles, collagen cross-linking agent, collagen, D-gluconolactone, sodium alginate and the antibacterial drug-loaded hydroxyapatite hollow microspheres are subjected to a pre-cross-linking reaction to obtain a pre-cross-linked mixture; The pre-crosslinking mixture is printed to obtain a primary bone scaffold; the primary bone scaffold and divalent copper ions are subjected to a re-crosslinking reaction to obtain the sodium alginate / collagen composite bone scaffold. The sodium alginate / collagen composite bone scaffold obtained by the preparation method of the present invention has good mechanical properties, relatively stable structure and antibacterial effect.

Disclosed herein are bone anchor assemblies and related methods that may provide improved fixation of a primary bone anchor. The bone anchor assembly may include a wing having a distal portion that may define a plurality of auxiliary bone anchor openings. Each auxiliary bone anchor opening may receive an auxiliary bone anchor that may enhance fixation of a primary bone anchor of the bone anchor assembly. The plurality of secondary bone anchor openings may be oriented such that when the wing is coupled to a primary bone anchor assembly of a vertebral segment in a first configuration, at least one secondary bone anchor may be driven to extend across a facet plane of the vertebral segment, and when coupled in a second configuration, at least one secondary bone anchor may be driven to extend across a facet plane of the vertebral segment. Each auxiliary bone anchor received within the wing may be driven to conform to the vertebral segment.

The present invention is for method of treating metabolic bone disease, new compounds and pharmaceutical compositions comprising the active ingredients having inhibition effects on osteoclast differentiation. The pharmaceutical composition comprising new compounds according to the present invention can be used as medicines for treating metabolic bone diseases such as bone metastatic cancer, solid cancer bone metastasis, musculoskeletal complication by solid cancer bone metastasis, hypercalcemia by malignant tumor, multiple myeloma, primary bone tumor, osteoporosis, rheumatoid arthritis, osteoarthritis, periodontal disease, inflammatory resorption of alveolar bone, inflammatory resorption of bone, and Paget's disease.