17 results about "Primary cancer" patented technology

Disclosed herein are methods of producing organoids. In some aspects, the methods involve producing organoids by implanting primary cancer cells such as glioblastoma cells into brain organoids, thereby producing brain cancer organoids. The method can comprise an incubation step in which the brain organoid is incubated with primary cancer cells in a hanging liquid droplet to produce the brain cancer organoid. In some aspects, the brain cancer organoids provide tools for understanding and treating cancers of the nervous system, such as glioblastoma.

A construct configured to express a payload that is harmful (e.g., cytotoxic and immunomodulatory) intracellularly, which can be used to treat or prevent a primary cancer / tumor and to stimulate, induce, generate, or promote an effective immune response against recurrent / metastatic cancer / tumor that may arise from this primary cancer / tumor, is disclosed.

The present invention relates to a composition for the prevention or treatment of metastatic cancer, capable of inducing an abscopal effect, which is a systemic anticancer effect, in combination with radiotherapy to treat metastatic cancer that has spread from a primary cancer, wherein, by having confirmed that, upon radiotherapy in which a primary cancer site was irradiated with a low dose of radiation, the abscopal effect, which is a systemic anticancer effect, did not appear, whereas, when type 1 interferon was administered in combination with low-dose radiation to the primary cancer site, an anticancer effect appears even in non-radiated metastatic cancer sites, a composition comprising type 1 interferon is provided as a therapeutic agent or adjuvant for the treatment of metastatic cancer, which induces a systemic anticancer effect upon low-dose radiotherapy.

The present invention relates to a DNA construct comprising a gene encoding a flagellin protein and a gene encoding an adjuvant protein. The present invention is an effective cancer treatment that selectively kills only cancer cells. The attenuated Salmonella strain is designed to produce an immunostimulatory substance in cancer tissue, thereby inducing a strong anti-cancer immune response. This can be useful as a preventive or therapeutic composition that significantly suppresses tumor size in primary cancers as well as metastatic cancers, thereby improving survival rates.

The present invention relates to a method and composition for inducing at least one immune stimulus, for treating primary cancer and cancer of cells dispersed in the human body and its metastases, and in particular for inducing and promoting the patient's own immune system to recognize and kill the cancer cells and establish a memory to prevent recurrence of the cancer disease.

The invention discloses a composition capable of achieving double benefits of treatment and immune memory and application of the composition, and belongs to the technical field of biological medicine, and the pharmaceutical composition is composed of 5-carboxyl-8-hydroxyquinoline or pharmaceutically acceptable salt thereof and an anti-tumor drug nano preparation. Preferably, the doxorubicin liposome is composed of 5-carboxyl-8-hydroxyquinoline or a pharmaceutically acceptable salt thereof and a sialic acid functional group modified doxorubicin liposome. The composition can promote antigen presenting cell functions, enhance T cell activity, strengthen T cell memory formation, induce deep remodeling of an immunosuppression microenvironment and training immunity of a congenital immune system, generate strong immune memory, realize comprehensive killing of homologous or heterologous tumors, and has a good application prospect. The recurrence of primary tumors, the generation of secondary primary cancers and the attack of other newborn cancers are prevented.

The present disclosure relates to an apparatus and a method for providing information for diagnosing micro-residual diseases through quantification of single-nucleotide variants in cfDNA. The apparatus according to the present disclosure comprises: a memory in which at least one process for performing an operation for providing information for diagnosing micro-residual diseases is stored; and a processor for performing an operation according to the process, wherein the processor analyzes WGS of patient-derived cancer tissues using two or more pieces of somatic variant analysis software to obtain data on the positions of consensus single-nucleotide variants commonly identified in primary cancer samples, matches reference sequences at the positions of the single-nucleotide variants with cfDNA WGS of the patient to obtain supporting reads matching in which the variants match in overlapping regions of paired reads, filters the obtained supporting reads on the basis of predetermined conditions, and generates probability data related to micro-residual diseases on the basis of the filtered supporting reads.

The present invention relates to a DNA construct comprising: a gene encoding flagellin and a gene encoding an immunopotentiator (or adjuvant) protein. In order to achieve effective cancer treatment by selectively killing only cancer cells, the attenuated Salmonella strains of the present disclosure are designed to produce immunogenic substances in cancer tissue to induce a strong anti-cancer immune response to significantly inhibit tumor size in primary and metastatic cancers. Therefore, the strain can be advantageously used in a prophylactic or therapeutic composition for increasing survival rate.

As disclosed herein, SIRPα is integral to immuno-evasion by many different cancer types as well as cancer resistance to therapies, and reducing SIRPα levels on can bolster antigen acquisition, processing, and presentation, decrease TME immunosuppression and thereby promote tumor-specific T cell activation to eliminate tumors and generate an adaptive immune response consisting of memory T cells, circulating antibodies, and plasma cells, all of which may be specific for neo-antigens in the original cancer. Therefore, disclosed are activated SIRPαlow macrophages that are useful for treating cancers.

The present disclosure relates to a chimeric adenovirus for detecting circulating tumor cells. An adenovirus type 5 / 3 for detecting circulating tumor cells, according to the present disclosure, comprises a knob domain of adenovirus type 3 and thus targets DSG2, and thus can also detect EMT circulating tumor cells, has excellent viability in the blood, and can accurately detect and isolate circulating tumor cells from more carcinomas, and thus enables early diagnosis of cancer and analysis of the type and stage of primary cancer, and can predict cancer prognosis, monitor cancer progression and analyze drug response and therapeutic effects through the genetic analysis of isolated circulating tumor cells, and therefore can be effectively used in personalized treatment.

The present application is directed to a combination therapy comprising a thyroid hormone receptor beta-1 (TKβ) agonist, and a primary cancer therapeutic. Methods of treating cancer and inducing differentiation in a population of cancer cells are also disclosed.

The application belongs to the technical field of biotechnology, and particularly discloses a method for extracting primary cancer-associated fibroblasts from renal clear cell carcinoma tissue. The method comprises the following steps: treating fresh renal clear cell carcinoma tissue at 0-4 DEG C, and only retaining a region rich in tumor cells and interstitial mixed; low-speed shearing the region rich in tumor cells and interstitial mixed into fragments with a volume of 1-3 mm 3 ; placing the fragments in a collagenase I solution, collecting cell precipitates after digestion is completed; inoculating the cell precipitates into a first culture medium, and replacing the fresh first culture medium every day within the first 3 days after inoculation, and then replacing the first culture medium every 2-3 days. The method overcomes the problems of low cell adhesion rate, serious contamination, low cell activity and low purity of the conventional extraction technology of primary cancer-associated fibroblasts when applied to renal clear cell carcinoma tissue, and can stably obtain primary cancer-associated fibroblasts with high purity and high survival rate within 7-10 days.

A cultivation method for simulated microgravity-induced reprogramming of primary cancer cells and related culture media and reagents are in the field of biotechnology. A multi-directional G-force generator is used to simulate a 10−3 g microgravity environment, inducing reprogramming of cancer / cancer-adjacent tissue cells derived from patients, maintaining stemness of primary cell populations, and achieving rapid, long-term, high-fidelity cultivation of tissue cells from patients. Using this cultivation method, 106 cells can be obtained within 7 days from a 1 mm3 tissue block acquired during surgery for drug screening. Specially formulated culture media, washing solutions, and digestion solutions are also provided, effectively solving problems of contamination susceptibility and difficult digestion of clinical samples. The established cancer cell models maintain highly similar genetic characteristics to the original tissue and can reflect differences in drug sensitivity between different patients, providing a powerful tool for precision cancer treatment.

The present invention relates to a method and a composition for inducing at least one immunostimulation for the treatment of primary cancer and cellular carcinoma dispersed in the human body and its metastases, in particular for inducing and promoting the immune system of the patient's own to identify and kill cancer cells and establish memory to prevent the recurrence of cancer diseases.

A cultivation method for simulated microgravity-induced reprogramming of primary cancer cells and related culture media and reagents are in the field of biotechnology. A multi-directional G-force generator is used to simulate a 10−3 g microgravity environment, inducing reprogramming of cancer / cancer-adjacent tissue cells derived from patients, maintaining stemness of primary cell populations, and achieving rapid, long-term, high-fidelity cultivation of tissue cells from patients. Using this cultivation method, 106 cells can be obtained within 7 days from a 1 mm3 tissue block acquired during surgery for drug screening. Specially formulated culture media, washing solutions, and digestion solutions are also provided, effectively solving problems of contamination susceptibility and difficult digestion of clinical samples. The established cancer cell models maintain highly similar genetic characteristics to the original tissue and can reflect differences in drug sensitivity between different patients, providing a powerful tool for precision cancer treatment.