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Primary Cancer. As noted above, primary cancer refers to the initial cancer a person experiences, whether that is lung cancer, breast cancer, or another type of cancer. It's possible to have more than one primary cancer; sometimes these primary cancers are found at the same time, and sometimes they occur decades apart (see below).
A method for extracting primary cancer-associated fibroblasts from renal clear cell carcinoma tissue
The application belongs to the technical field of biotechnology, and particularly discloses a method for extracting primary cancer-associated fibroblasts from renal clear cell carcinoma tissue. The method comprises the following steps: treating fresh renal clear cell carcinoma tissue at 0-4 DEG C, and only retaining a region rich in tumor cells and interstitial mixed; low-speed shearing the region rich in tumor cells and interstitial mixed into fragments with a volume of 1-3 mm 3 ; placing the fragments in a collagenase I solution, collecting cell precipitates after digestion is completed; inoculating the cell precipitates into a first culture medium, and replacing the fresh first culture medium every day within the first 3 days after inoculation, and then replacing the first culture medium every 2-3 days. The method overcomes the problems of low cell adhesion rate, serious contamination, low cell activity and low purity of the conventional extraction technology of primary cancer-associated fibroblasts when applied to renal clear cell carcinoma tissue, and can stably obtain primary cancer-associated fibroblasts with high purity and high survival rate within 7-10 days.
Owner:XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV