Separation preparation method for human primary cancer cells

Abstract

The invention provides a separation preparation method for human primary cancer cells. According to the separation preparation method, the culture process is divided into two periods, culture is carried out with culture mediums with fetal calf serum of different concentrations respectively, the concentrations of the fetal calf serum in the culture mediums are increased gradually, then the adaptability and the activity of primary cancer cells are improved gradually, relatively rapid cell proliferation and relatively high yield are achieved, and meanwhile good hereditary stability is achieved; due to addition of sodium pyruvate, MaxCitro, Y-27632 and griseofulvin, bacterial and fungal infection can be prevented, cell proliferation can be promoted, and cell differentiation can be inhibited; and due to addition of a plant source recombinant human serum albumin in a cryoprotectant, a part of fetal calf serum can be replaced, the use amount of the fetal calf serum can be reduced, and thus the cost can be lowered on premise that the effect is not affected.

Description

technical field [0001] The invention belongs to the technical field of primary cell culture, in particular to a method for separating and preparing human primary tumor cells. Background technique [0002] Cell culture is one of the most commonly used methods in biological and medical research, and can be divided into two types: primary culture and subculture. Primary culture, also known as primary culture, is a part of a tissue or organ directly obtained from an organism, which is dispersed into individual cells by mechanical methods or various enzymes (usually trypsin) and chelating agents (usually EDTA) treatment, adding The appropriate amount of culture medium is placed in a suitable culture container, under sterile, appropriate temperature and certain conditions, to enable it to survive, grow and reproduce. [0003] Strictly speaking, primary culture refers to the inoculation and culture of tissues taken from the body to the first subculture stage; but in fact, the cult...

AI Technical Summary

Problems solved by technology

However, the cost of serum is high. If the medium containing the same concentration of serum is used throughout the culture process, the cost will be too high and large-scale culture cannot be carried out; if the concentration of serum is reduced, it may lead to insufficient nutrient supply and affect cell proliferation.

Images