62 results about "Synthetic Cells" patented technology

The invention discloses a preparation method of an artificial cell coated with a macrophage membrane, and belongs to the technical field of artificial cell preparation. The preparation method comprises the following steps: dissolving poly (diallyldimethylammonium chloride) and sulfobutyl-beta-cyclodextrin in ultrapure water, and mixing an aqueous solution of the poly (diallyldimethylammonium chloride) and an aqueous solution of the sulfobutyl-beta-cyclodextrin to obtain a PDDA / SBE-beta-CD condensation liquid droplet solution; and mixing the macrophage membrane suspension and the PDDA / SBE-beta-CD condensed fluid drops in an equal mass ratio, and realizing in-situ coating of the macrophage membrane by adopting an ultrasonic coating technology to obtain the artificial cell coated with the macrophage membrane. The PDDA / SBE-beta-CD condensation liquid droplet constructed by the invention has good stability under the conditions of physiological salt concentration and pH and at 37 DEG C; the artificial cell coated with the macrophage membrane is prepared by mixing with the macrophage membrane suspension, and the macrophage membrane serving as a shell not only can prevent aggregate aggregation, but also can improve the structural stability and biocompatibility.

The invention discloses a new enzyme with acylsulfonate transferase activity or a mutant thereof and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, the activity of acylsulfonate transferase is measured, and a corresponding mutant is obtained by using a directed evolution method. Compared with reported mouse acyl sulfonate transferase (ASTIV), the enzyme sequence obtained in the invention has better catalytic activity in a regeneration system of PAPS, and provides a new choice for enzymatic synthesis of PAPS.

The invention discloses acyl sulfonic acid transferase and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means to obtain the acyl sulfonate transferase derived from Podarcismuris, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, and the activity of the acyl sulfonate transferase is measured. Compared with reported mouse acyl sulfonate transferase, the acyl sulfonate transferase provided by the invention has better catalytic activity in a regeneration system of PAPS, and a new choice is provided for enzymatic synthesis of PAPS.

The present invention addresses the problem of providing a composition for lyophilizing extracellular vesicles or artificial cell vesicles, which makes it possible to prevent the extracellular vesicles or the artificial cell vesicles from being deteriorated in shape preserving properties or functions thereof or from being reduced in particle diameters when the extracellular vesicles or the artificial cell vesicles are recovered by hydration after lyophilization. The composition for lyophilizing extracellular vesicles or artificial cell vesicles is prepared, the composition being characterized by being intended to be added to extracellular vesicles or artificial cell vesicles upon use and also being characterized by containing the following hydrophobic substances: (a) phenylalanine or a methyl ester thereof; (b) a dipeptide which is composed of one or two kinds of hydrophobic amino acids selected from the group consisting of phenylalanine, leucine, glycine, valine, isoleucine, tryptophan, and alanine and contains at least one phenylalanine molecule as a constituent amino acid; and the like.

The application discloses a kind of artificial cells for single-cell mass spectrometry and preparation method thereof, wherein artificial cell includes internal water phase, intermediate oil phase and external water phase;Internal water phase includes polyethylene glycol and polyvinyl alcohol aqueous solution, intermediate oil phase includes L-alpha-phosphatidylcholine chloroform and hexane mixture;External water phase includes PVA and F-68 aqueous solution.Compared with natural cell sample, the novel artificial single cell based on microfluidic self-assembly developed in the application has better uniformity, stability and controllability, effectively avoids significant measurement difference between single-cell individual samples, and effectively solves the problem of difficult stable preservation of biological samples.The proposed artificial cell preparation method will largely solve the problem of lack of standard reference material in the field of single-cell mass spectrometry, making the results of single-cell mass spectrometry method more accurate, reliable and mutually recognized.

The present invention relates to a functionalized artificial cell based on a composite coacervate, preparation and application thereof, and relates to the field of biomedicine technology. The preparation method is as follows: a positively charged solution and a negatively charged solution are mixed, the positively charged solution being a polylysine solution or an ε-polylysine hydrochloride solution, and the negatively charged solution being a deoxyribonucleic acid solution; a coacervate droplet suspension containing functional components is obtained by liquid-liquid phase separation; phospholipids and PEGylated phospholipids are dissolved in an organic solvent and then subjected to vacuum rotary evaporation until the organic solvent evaporates to obtain a lipid membrane; the coacervate droplet suspension is added to the lipid membrane for hydration until the lipid membrane is completely dissolved, thereby obtaining a functionalized artificial cell; at least one of the positively charged solution, the negatively charged solution and the phospholipid solution contains a functional component. The functionalized artificial cell based on the composite coacervate constructed by the present invention has a cell membrane-like and cytoplasm-like structure, and has good physiological stability and biocompatibility.

The invention belongs to the technical field of biotechnology, and discloses a peanut peptide for promoting cell development, which is characterized in that the sequence of the peanut peptide is Met Ala Ser Val Thr Gly Pro Ser Ser Ala Leu Ala Asp Ser Ile Gly, through the accurately designed amino acid sequence, the peanut peptide constructs a molecular level adaptation mechanism with a cell surface receptor, triggers a cascade reaction of a key signal channel of phosphatidylinositol-3 kinase PI3K, and can promote cell development. The activation effect not only accelerates the process of a cell cycle and increases the proportion of DNA synthetic cells in an S phase by 40% or more, but also directionally induces the mesenchymal stem cells to differentiate into functional cells such as muscle cells and nerve cells by up-regulating transcription factors MyoD and NeuroD1, in a three-dimensional cell culture model, the cell migration rate of an experimental group added with 200 [mu] g / mL peanut peptide is increased by 58% compared with a control group, and the cell migration rate of the experimental group added with 200 [mu] g / mL peanut peptide is increased by 30% or more compared with that of the control group. The branch density of the formed vascular-like network is increased by 3 times, and meanwhile, the test further proves that the peanut peptide has the function of promoting cell development and shows remarkable optimization capability on a tissue regeneration microenvironment.

The invention discloses a multi-chamber vesicle as well as a preparation method and application thereof. Each multi-chamber vesicle is an anionic liposome, and a cavity of the anionic liposome contains a plurality of cationic liposomes and a buffer solution containing Mg < 2 + >. According to the preparation method of the multi-chamber vesicle, a special oil phase and a water phase with different charges are introduced, so that the multi-chamber vesicle in which a plurality of cationic liposomes are wrapped by a single anionic liposome is successfully prepared. The multi-chamber vesicle is good in biocompatibility, controllable in particle size and stable in mechanical property, and provides technical support for preparation of artificial cells and novel drug carriers in the future.

The invention provides a basic model construction method for pathological image high-resolution generation based on cell topology guidance, which comprises the following steps of: prompting a large language model based on an interactive learning scheme, and generating synthetic cell arrangement according to real cell arrangement by utilizing a cell arrangement generator; integrating the synthetic cell arrangement as a condition into a potential diffusion model; a high resolution pathology image containing cellular level control is generated through a potential diffusion model. The method has excellent performance in the aspects of authenticity of the synthetic image and retention of diagnostic information, and can significantly improve the performance of lesion subtype classification and segmentation tasks. The method not only enhances the authenticity of medical image synthesis, but also provides a high-quality data enhancement tool for downstream tasks, and promotes the development of artificial intelligence in the field of medical care.

The utility model relates to the technical field of protein extraction, and discloses a protein extraction scraper device which comprises a base, a scraper assembly is arranged at the top of the base, the scraper assembly comprises fixing plates, a sliding groove, a rotating shaft, a first connecting rod, a second connecting rod, a connecting plate, a scraper mechanism, a guide rail and a sliding block, and the fixing plates are arranged on the two sides of the top of the base; sliding grooves are formed in the fixing plates, a rotating shaft is arranged between the two fixing plates, and first connecting rods are arranged at the two ends of the rotating shaft. According to the protein extraction scraper device, by arranging the scraper assembly, manual cell scraping can be replaced, traditional cell scraping is replaced, the labor intensity of workers is reduced, a driving motor is started to be matched with a rotating shaft, a first connecting rod, a second connecting rod and a connecting plate, and a scraper mechanism can be driven to move downwards; the stepping motor is started to drive the scraper blade to rotate, and bottom cells of the culture dish are scraped.

A synthetic cytokine signaling system includes an engineered cytokine signaling chain encoding a transmembrane polypeptide that contains a particular variant intracellular domain of IL9R and an extracellular domain of a cytokine receptor such as IL2Rβ, IL4Rα, IL7Rα, IL9R, and IL21R. Depending on whether a functionally compatible cytokine ligand domain is fused on the N-terminus, the engineered cytokine signaling chain may be in a self-activating form, or optionally may be in a non-self-activating form. In the latter case, the synthetic cytokine signaling system may further include an engineered cytokine ligand chain encoding a corresponding cytokine ligand that can be membrane-bound or secreted. Immune cells modified with the synthetic cytokine signaling system displays improved characteristics compared with unmodified immune cells, such as the zero or reduced dependency on IL-2 when cultured in vitro, enhanced activation upon antigen stimulation, increased cytotoxicity against target cells, and improved tumor control capacity when transferred in vivo.

The present invention relates to a device for water-in-air generation of droplets comprising a collection vessel, a droplet generation device positioned above the collection vessel comprising a piezoelectric transducer (21) and at least one capillary (22) with a nozzle (23) pointing downwards to the collection vessel; a laser source (30) generating a visible light laser beam (31) with tophat profile, and an imaging setup for continuously monitoring the droplet generation (40). Said device is particularly useful for preparing monodisperse giant unilamellar vesicles of any composition and three-dimensional microenvironment niches for cells or synthetic cells in high throughput.

The present invention relates to a recombinant expression vector encoding an inducible cell death switch, a pH-stable fluorophore and a pH-sensitive fluorophore. Moreover, the invention relates to cells comprising said recombinant expression vector as well as their use in an in vitro phagocytosis assay.

The present invention includes a method and kit for cDNA synthesis of a 3′UTR / poly(A) tail junction of cellular RNA comprising: obtaining RNA comprising a 3′UTR / poly(A) junction and a poly(a) tail; combining the RNA with three terminating nucleotides of modified-deoxyGTP, modified-deoxyCTP and modified-deoxyATP, dNTPs, and adaptor sequence-oligo-dT; performing reverse transcription of the RNA with a reverse transcriptase primed with the adaptor sequence-oligo-dT to form terminated cDNA fragments that are stochastically terminated upstream of the 3′UTR / poly(A) junction, but not within the poly(A) tail; isolating the terminated cDNA fragments; chemically ligating a functionalized 5′ adaptor to the terminated cDNA; and amplifying the chemically-ligated cDNA into an amplification product, wherein the cDNA is enriched for sequences at the 3′UTR / poly(A) tail junction without fragmentation or enzymatic ligation.

A method is provided for monitoring a cell culture in a culture medium in a container (3) by means of a computer (2) having software (5) wherein the container (3) is connected to at least one sensor (6) for measuring the cell culture (5), the software (5) uses the spectral data measured by the at least one sensor (6) to create and operate a data model (8) to calculate specific process parameters of the cell culture, in which first a synthetic cell-free culture medium sample is measured with the at least one sensor (6); comprising different ranges of culture medium compounds and / or metabolites and / or products and / or process parameter combinations, and then real-time monitoring of target cell culture parameters is performed using the data model (8) trained by the software (5).

Provided herein are compositions and methods comprising a synthetic cell that generates adenosine triphosphate (ATP) and / or nicotinamide adenine dinucleotide (NADH). In particular, the present invention relates to the compositions and methods comprising synthetic cells that generate ATP and / or NADH from the uptake of glucose.

The invention provides an assembling mode of RNA liquid drops deformed through photostimulation and application of the RNA liquid drops, and belongs to the technical field of preparation of nucleic acid for analysis. The method comprises the following steps: mixing an RNA single strand with a buffer solution through structural design to obtain a mixed system with a specific concentration; after the mixed system is subjected to heat shock to complete base pairing and assembling, sealing and packaging are performed, the RNA liquid drops deformed through photostimulation are obtained, and the diameter of the liquid drops is 7.0-13.0 microns. The RNA liquid drop obtained in the embodiment can deform under light stimulation, behavior regulation and control can be achieved through group modification, and feasibility is provided for construction and functional expression of artificial cells.

The invention discloses a preparation method of a bionic system based on a microfluidic technology, and relates to the technical field of functional bionic materials. Chitosan, nano-enzyme, alginate, phytic acid and the like are taken as raw materials, the artificial cell system with adjustable morphology, structure and activity is prepared in a high-throughput manner by utilizing a micro-fluidic chip through an intermolecular polymerization reaction, and the prepared artificial cell system can simulate a glucose metabolism function.

The AI platform is used to develop a combination therapy for patients suffering from cloned tumors. Comprising at least two perturbations is capable of targeting clones, including subclones, that evade therapeutic interventions due to resistance and / or evolution. The AI platform is trained with perturbation data obtained from at least one cell line having similar characteristics to the clone of interest. The trained AI platform predicts how the clone of interest responds to the perturbation and ranks the perturbation responses from highest to lowest. At least one cell line may be an existing cell line from a well established database or a synthetic cell line generated by an AI platform. The AI platform may include one or more of a machine learning platform, a deep learning platform, an artificial neural network (ANN), a convolutional neural network (CNN), and a generative adversarial network (GAN).

The invention relates to a carbon nanotube embedded artificial cell, a preparation method thereof and an application of the carbon nanotube embedded artificial cell in degradation of antibiotic resistance genes, and belongs to the technical field of environmental functional materials. According to the material, an artificial cell membrane formed by lipidosome serves as a shell, layered double-oxide hollow spheres derived from ferrocobalt layered double hydroxides are packaged in the shell, and carbon nanotubes are embedded into the artificial cell membrane to serve as a transmission channel. The preparation method comprises the following steps: preparing carbon spheres by a hydrothermal method, preparing ferrocobalt layered double hydroxides by a coprecipitation method, carrying out composite calcination to obtain layered double oxide hollow spheres, and finally constructing artificial cells by a film hydration method and embedding the artificial cells into the carbon nanotubes. The material can be used for catalytically degrading antibiotic resistance genes in water, singlet oxygen and superoxide free radicals are generated through peroxymonosulfate activation, gene segments are effectively attacked, and efficient degradation is achieved. The method has the advantages of efficient catalytic activity and confinement encapsulation, magnetic recovery is facilitated, and a novel environment-friendly method is provided for treatment of antibiotic resistance genes.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and verified by this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate compound for cancer prevention or treatment.

The invention discloses an artificial cell and a preparation method thereof as well as a bionic tissue and a preparation method thereof, an aqueous dispersion of a negative electricity nanometer material is sprayed into an aqueous phase receiving bath containing a cationic polyelectrolyte and a surfactant through a gas-liquid shearing microfluidic technology, and one-step construction of an artificial cell membrane and a cytoskeleton is realized. According to the method, the internal skeleton enhanced artificial cell is constructed in a water phase by a one-step method by utilizing the electrostatic interaction of the negative electricity nano material and the cationic polyelectrolyte, and the method has the characteristics of convenience in preparation and excellent stability. By introducing different functional materials, multifunctional integration of the artificial cell can be realized. Meanwhile, the adhesion inducer is added, so that a bionic tissue can be further constructed, and the bionic tissue integrates excellent mechanical properties of the artificial cells and can bear disturbance to a certain degree without disintegration.

An AI platform is used to develop a combination therapy for a patient suffering from a tumor that has formed clones. The combination therapy, containing at least two disturbances, is capable of targeting clones (including subclones) that have evaded therapeutic intervention due to resistance and / or evolution. The AI ​​platform is trained on disturbance data obtained from at least one cell line that has similar properties to a relevant clone. The trained AI platform predicts how the relevant clone will respond to disturbances and ranks the disturbance responses from strongest to weakest. At least one cell line can be an existing cell line from an established database or a synthetic cell line generated by the AI ​​platform.The AI ​​platform can include one or more from a machine learning platform, a deep learning platform, an artificial neural network (ANN), a convolutional neural network (CNN), and a generative adversarial network (GAN).

The invention belongs to the technical field of functional nano materials and biomimetic catalysis, and discloses a universal preparation method and application of high-entropy nano enzyme. According to the method, various metal salts, polyvinylpyrrolidone, benzyl alcohol and formaldehyde are used as raw materials, and various high-entropy alloy nanoparticles and nanosheets are prepared under the same reaction condition through a solvothermal method. The prepared high-entropy nano-enzyme has the advantages of controllable composition, uniform morphology, high catalytic activity and the like, can simulate the activity of various natural enzymes, and is suitable for the fields of artificial cell systems, biological catalysis, sensing and the like.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and validated in this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate drug for cancer prevention or treatment.

The disclosure provides synthetic cytotoxic cells, comprising a hydrogel microsphere and a TNF-related apoptosis-inducing ligand (TRAIL), or active fragment thereof. In aspects, the TRAIL or active fragment thereof is attached to the surface of the hydrogel microsphere. The disclosure further provides methods of preparing the synthetic cytotoxic cells and methods of use thereof in the treatment of cancer.

The application of carbon nanotubes in miRNAs detection and cell imaging, the application uses carbon nanotube nanomaterial as an effective artificial transmission channel, promotes the signal and ion transmission of liposome artificial cell to cell membrane. The addition amount of carbon nanotubes will affect the efficiency of membrane fusion, the application studies the addition amount of carbon nanotubes and the ion channel function of carbon nanotubes in the whole system.

Methods of synthesizing annotated cell microscopy images are provided, comprising: inputting a random noise vector into a deep learning model trained using real cell microscopy images and corresponding greyscale segmentation masks, to generate synthetic cell microscopy images and corresponding greyscale segmentation masks. Related methods, products and systems are also described.

Some embodiments provide for a method of using a trained machine learning model to predict a characteristic of a subject, the method comprising: processing cfRNA expression data using the trained machine learning model to obtain an output indicative of the characteristic of the subject, wherein the trained machine learning model was trained using artificial cfRNA expression data, the artificial cfRNA expression data comprising a plurality of artificial cfRNA expression profiles, an artificial cfRNA expression profile having been generated by: generating a healthy expression profile component; generating a tumor expression profile component; and generating the artificial cfRNA expression profile using the healthy expression profile component and the tumor expression profile component.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.