106 results about "Synthetic Cells" patented technology

The present invention relates to chimeric antigen receptors (CAR) containing TCR hinge and transmembrane domains. The present invention also relates to using transmembrane pairing to enhance the stability and specificity of dual antigen CARs. The present invention also relates to new CARs containing transmembrane and signaling domains from cytokine receptors.

The present invention relates to a Synthetic Cellular Advanced Signal Adapter (SCASA) system and method comprising a genetically modified yeast cell capable of modulating a receiver cell, preferably a human immune cell. The invention provides systems to dynamically modulate the phenotype of the receiver cell in response to dynamic stimulation of the genetically modified yeast cell.

Provided herein are synthetic cytokine receptors, wherein each synthetic cytokine receptor comprises an extracellular domain, a transmembrane domain, and an interleukin 9 receptor (IL-9R) intracellular domain. Also provided herein are engineered cells expressing one or more such synthetic cytokine receptors.

The disclosure provides synthetic cytotoxic cells, comprising a hydrogel microsphere and a TNF-related apoptosis-inducing ligand (TRAIL), or active fragment thereof. In aspects, the TRAIL or active fragment thereof is attached to the surface of the hydrogel microsphere. The disclosure further provides methods of preparing the synthetic cytotoxic cells and methods of use thereof in the treatment of cancer.

The invention discloses a preparation method of an artificial cell coated with a macrophage membrane, and belongs to the technical field of artificial cell preparation. The preparation method comprises the following steps: dissolving poly (diallyldimethylammonium chloride) and sulfobutyl-beta-cyclodextrin in ultrapure water, and mixing an aqueous solution of the poly (diallyldimethylammonium chloride) and an aqueous solution of the sulfobutyl-beta-cyclodextrin to obtain a PDDA / SBE-beta-CD condensation liquid droplet solution; and mixing the macrophage membrane suspension and the PDDA / SBE-beta-CD condensed fluid drops in an equal mass ratio, and realizing in-situ coating of the macrophage membrane by adopting an ultrasonic coating technology to obtain the artificial cell coated with the macrophage membrane. The PDDA / SBE-beta-CD condensation liquid droplet constructed by the invention has good stability under the conditions of physiological salt concentration and pH and at 37 DEG C; the artificial cell coated with the macrophage membrane is prepared by mixing with the macrophage membrane suspension, and the macrophage membrane serving as a shell not only can prevent aggregate aggregation, but also can improve the structural stability and biocompatibility.

Methods and apparatus for generating an updated machine learning model for predicting a property of an energy storage system. The method includes receiving sensor data associated with an energy storage system, transforming, using at least one hardware computer processor, the sensor data into cell-level data for a number of representative cells in the energy storage system, generating, using at least one model of cell degradation and the cell-level data, synthetic cell-level data for each of the representative cells, training a machine learning (ML) model based on the cell-level data and the synthetic cell-level data, and outputting the trained ML model, wherein the trained ML model is configured to predict a property of the energy storage system based on cell-level data.

Provided are compositions and methods for a cell population comprising engineered cytotoxic innate lymphoid cells engineered for controlled expansion and / or activity, the engineered cytotoxic innate lymphoid cells comprising a synthetic cytokine receptor for a non-physiological ligand. The cytokine receptor may comprise a synthetic gamma chain polypeptide as a first dimerization domain, a first transmembrane domain, and an interleukin-2 receptor subunit gamma (IL-2RG) intracellular domain and a synthetic beta chain polypeptide as a second dimerization domain, a second transmembrane domain, and an intracellular domain selected from an interleukin-2 receptor subunit beta (IL-2RB) intracellular domain, an interleukin-7 receptor subunit beta (IL-7RB) intracellular domain, and / or an interleukin-21 receptor subunit beta (IL-21RB) intracellular domain. The non-physiological ligand activates the synthetic cytokine receptor in the cytotoxic innate lymphoid cells to induce expansion and / or activation of the engineered cytotoxic innate lymphoid cells.

Provided are methods and compositions for in situ detection of RNA in cells that does not require either the use of reverse transcriptase nor use of droplets. The methods can comprise annealing, in fixed and permeabilized cells, a pair of polynucleotide probes to adjacent sequences in a target RNA, which are subsequently ligated. A cell-specific barcode sequence can be subsequently synthesized in the cell using split-pool rounds to add barcode sequences to the ligated probe pair sequences in the cells, wherein an effect of multiple rounds of the split pooling is that ligated probe pair sequences in different cells have unique barcodes that are cell-specific.

The invention discloses a new enzyme with acylsulfonate transferase activity or a mutant thereof and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, the activity of acylsulfonate transferase is measured, and a corresponding mutant is obtained by using a directed evolution method. Compared with reported mouse acyl sulfonate transferase (ASTIV), the enzyme sequence obtained in the invention has better catalytic activity in a regeneration system of PAPS, and provides a new choice for enzymatic synthesis of PAPS.

The invention discloses acyl sulfonic acid transferase and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means to obtain the acyl sulfonate transferase derived from Podarcismuris, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, and the activity of the acyl sulfonate transferase is measured. Compared with reported mouse acyl sulfonate transferase, the acyl sulfonate transferase provided by the invention has better catalytic activity in a regeneration system of PAPS, and a new choice is provided for enzymatic synthesis of PAPS.

Provided are polynucleotides encoding inactivated cell surface receptors. Also provided are genetically engineered induced pluripotent stem cells (iPSCs) and derivative cells thereof expressing a chimeric antigen receptor (CAR) and methods of using the same. Also provided are compositions, polypeptides, vectors, and methods of manufacturing.

The present invention addresses the problem of providing a composition for lyophilizing extracellular vesicles or artificial cell vesicles, which makes it possible to prevent the extracellular vesicles or the artificial cell vesicles from being deteriorated in shape preserving properties or functions thereof or from being reduced in particle diameters when the extracellular vesicles or the artificial cell vesicles are recovered by hydration after lyophilization. The composition for lyophilizing extracellular vesicles or artificial cell vesicles is prepared, the composition being characterized by being intended to be added to extracellular vesicles or artificial cell vesicles upon use and also being characterized by containing the following hydrophobic substances: (a) phenylalanine or a methyl ester thereof; (b) a dipeptide which is composed of one or two kinds of hydrophobic amino acids selected from the group consisting of phenylalanine, leucine, glycine, valine, isoleucine, tryptophan, and alanine and contains at least one phenylalanine molecule as a constituent amino acid; and the like.

The application discloses a kind of artificial cells for single-cell mass spectrometry and preparation method thereof, wherein artificial cell includes internal water phase, intermediate oil phase and external water phase;Internal water phase includes polyethylene glycol and polyvinyl alcohol aqueous solution, intermediate oil phase includes L-alpha-phosphatidylcholine chloroform and hexane mixture;External water phase includes PVA and F-68 aqueous solution.Compared with natural cell sample, the novel artificial single cell based on microfluidic self-assembly developed in the application has better uniformity, stability and controllability, effectively avoids significant measurement difference between single-cell individual samples, and effectively solves the problem of difficult stable preservation of biological samples.The proposed artificial cell preparation method will largely solve the problem of lack of standard reference material in the field of single-cell mass spectrometry, making the results of single-cell mass spectrometry method more accurate, reliable and mutually recognized.

The present invention relates to a functionalized artificial cell based on a composite coacervate, preparation and application thereof, and relates to the field of biomedicine technology. The preparation method is as follows: a positively charged solution and a negatively charged solution are mixed, the positively charged solution being a polylysine solution or an ε-polylysine hydrochloride solution, and the negatively charged solution being a deoxyribonucleic acid solution; a coacervate droplet suspension containing functional components is obtained by liquid-liquid phase separation; phospholipids and PEGylated phospholipids are dissolved in an organic solvent and then subjected to vacuum rotary evaporation until the organic solvent evaporates to obtain a lipid membrane; the coacervate droplet suspension is added to the lipid membrane for hydration until the lipid membrane is completely dissolved, thereby obtaining a functionalized artificial cell; at least one of the positively charged solution, the negatively charged solution and the phospholipid solution contains a functional component. The functionalized artificial cell based on the composite coacervate constructed by the present invention has a cell membrane-like and cytoplasm-like structure, and has good physiological stability and biocompatibility.

The invention belongs to the technical field of biotechnology, and discloses a peanut peptide for promoting cell development, which is characterized in that the sequence of the peanut peptide is Met Ala Ser Val Thr Gly Pro Ser Ser Ala Leu Ala Asp Ser Ile Gly, through the accurately designed amino acid sequence, the peanut peptide constructs a molecular level adaptation mechanism with a cell surface receptor, triggers a cascade reaction of a key signal channel of phosphatidylinositol-3 kinase PI3K, and can promote cell development. The activation effect not only accelerates the process of a cell cycle and increases the proportion of DNA synthetic cells in an S phase by 40% or more, but also directionally induces the mesenchymal stem cells to differentiate into functional cells such as muscle cells and nerve cells by up-regulating transcription factors MyoD and NeuroD1, in a three-dimensional cell culture model, the cell migration rate of an experimental group added with 200 [mu] g / mL peanut peptide is increased by 58% compared with a control group, and the cell migration rate of the experimental group added with 200 [mu] g / mL peanut peptide is increased by 30% or more compared with that of the control group. The branch density of the formed vascular-like network is increased by 3 times, and meanwhile, the test further proves that the peanut peptide has the function of promoting cell development and shows remarkable optimization capability on a tissue regeneration microenvironment.

The invention discloses a multi-chamber vesicle as well as a preparation method and application thereof. Each multi-chamber vesicle is an anionic liposome, and a cavity of the anionic liposome contains a plurality of cationic liposomes and a buffer solution containing Mg < 2 + >. According to the preparation method of the multi-chamber vesicle, a special oil phase and a water phase with different charges are introduced, so that the multi-chamber vesicle in which a plurality of cationic liposomes are wrapped by a single anionic liposome is successfully prepared. The multi-chamber vesicle is good in biocompatibility, controllable in particle size and stable in mechanical property, and provides technical support for preparation of artificial cells and novel drug carriers in the future.

The invention provides a basic model construction method for pathological image high-resolution generation based on cell topology guidance, which comprises the following steps of: prompting a large language model based on an interactive learning scheme, and generating synthetic cell arrangement according to real cell arrangement by utilizing a cell arrangement generator; integrating the synthetic cell arrangement as a condition into a potential diffusion model; a high resolution pathology image containing cellular level control is generated through a potential diffusion model. The method has excellent performance in the aspects of authenticity of the synthetic image and retention of diagnostic information, and can significantly improve the performance of lesion subtype classification and segmentation tasks. The method not only enhances the authenticity of medical image synthesis, but also provides a high-quality data enhancement tool for downstream tasks, and promotes the development of artificial intelligence in the field of medical care.

The present invention relates to an improved protein-based biosensor suitable for the detection of one or more target molecules in a sample. The biosensor is fully reversible with a dynamic range suitable for analytical and diagnostic applications. The biosensor of the present invention can be used in synthetic biology, for example in the construction of artificial cellular or extracellular signaling networks.

A 1,6-diynes compound, its preparation method, and products obtained from 1,6-diynes as raw material and their applications are disclosed, belonging to the field of organic synthesis. The compound represented by a general formula (I), and its tautomers, polymorphs, solvates, or salts thereof, The 1,6-diyne compounds provided in the present application have variable structures, are easy to be synthesized, and are suitable for industrial production; they can be used for the synthesis of a class of benzoisoindole dimer compounds and their derivatives, cell imaging, and detecting reactive oxygen species (such as sodium hypochlorite, hydrogen peroxide, ozone, etc.). The 3-arylbenzoisoindole compound provided in the present application can be used as an important compound and play an important role in pharmaceuticals or fluorescent materials.

The utility model relates to the technical field of protein extraction, and discloses a protein extraction scraper device which comprises a base, a scraper assembly is arranged at the top of the base, the scraper assembly comprises fixing plates, a sliding groove, a rotating shaft, a first connecting rod, a second connecting rod, a connecting plate, a scraper mechanism, a guide rail and a sliding block, and the fixing plates are arranged on the two sides of the top of the base; sliding grooves are formed in the fixing plates, a rotating shaft is arranged between the two fixing plates, and first connecting rods are arranged at the two ends of the rotating shaft. According to the protein extraction scraper device, by arranging the scraper assembly, manual cell scraping can be replaced, traditional cell scraping is replaced, the labor intensity of workers is reduced, a driving motor is started to be matched with a rotating shaft, a first connecting rod, a second connecting rod and a connecting plate, and a scraper mechanism can be driven to move downwards; the stepping motor is started to drive the scraper blade to rotate, and bottom cells of the culture dish are scraped.

Phosphorus is one of major elements necessary for growth and development of plants, and plays a crucial role in physiological processes and metabolic activities of the plants, such as energy metabolism, nucleic acid synthesis, stability of cell membrane structures, signal transduction and the like. The invention provides a wheat phosphorus absorption and utilization transcription factor TraesCS3D01G144700 and an application of a coding gene of the wheat phosphorus absorption and utilization transcription factor TraesCS3D01G144700 in response to low phosphorus stress. The invention provides new gene resources and technical means for improving the growth and yield of wheat under the condition of low-phosphorus soil and improving the phosphorus utilization efficiency and yield of wheat, and plays an important role in realizing the sustainable development of agriculture.

A synthetic cytokine signaling system includes an engineered cytokine signaling chain encoding a transmembrane polypeptide that contains a particular variant intracellular domain of IL9R and an extracellular domain of a cytokine receptor such as IL2Rβ, IL4Rα, IL7Rα, IL9R, and IL21R. Depending on whether a functionally compatible cytokine ligand domain is fused on the N-terminus, the engineered cytokine signaling chain may be in a self-activating form, or optionally may be in a non-self-activating form. In the latter case, the synthetic cytokine signaling system may further include an engineered cytokine ligand chain encoding a corresponding cytokine ligand that can be membrane-bound or secreted. Immune cells modified with the synthetic cytokine signaling system displays improved characteristics compared with unmodified immune cells, such as the zero or reduced dependency on IL-2 when cultured in vitro, enhanced activation upon antigen stimulation, increased cytotoxicity against target cells, and improved tumor control capacity when transferred in vivo.

The present invention relates to a device for water-in-air generation of droplets comprising a collection vessel, a droplet generation device positioned above the collection vessel comprising a piezoelectric transducer (21) and at least one capillary (22) with a nozzle (23) pointing downwards to the collection vessel; a laser source (30) generating a visible light laser beam (31) with tophat profile, and an imaging setup for continuously monitoring the droplet generation (40). Said device is particularly useful for preparing monodisperse giant unilamellar vesicles of any composition and three-dimensional microenvironment niches for cells or synthetic cells in high throughput.

The invention relates to the technical field of cell segmentation fine tuning, and discloses a cell segmentation fine tuning method, which comprises the following steps: collecting different types of cell image data and preprocessing; a small amount of labeled data and a large amount of unlabeled data are used, a high-quality synthetic cell image is generated through a GAN model, and a training set is expanded; a U-Net model is adopted, the basic model is migrated to a cell segmentation task, and a small amount of annotation data is utilized for fine adjustment; a multi-modal feature extraction method is adopted to extract features from a light microscope image and an electron microscope image respectively, and a feature fusion technology is introduced to fuse the features of different modals. According to the cell segmentation fine tuning method, the image data of different types of cells are collected from various sources and are preprocessed, including operations of noise reduction, smoothing, binarization and the like, so that the quality and consistency of the data are ensured, the subsequent data processing and model training are more efficient and accurate, and noise interference can be effectively reduced by preprocessing the image.

The present invention relates to a recombinant expression vector encoding an inducible cell death switch, a pH-stable fluorophore and a pH-sensitive fluorophore. Moreover, the invention relates to cells comprising said recombinant expression vector as well as their use in an in vitro phagocytosis assay.

The present invention includes a method and kit for cDNA synthesis of a 3′UTR / poly(A) tail junction of cellular RNA comprising: obtaining RNA comprising a 3′UTR / poly(A) junction and a poly(a) tail; combining the RNA with three terminating nucleotides of modified-deoxyGTP, modified-deoxyCTP and modified-deoxyATP, dNTPs, and adaptor sequence-oligo-dT; performing reverse transcription of the RNA with a reverse transcriptase primed with the adaptor sequence-oligo-dT to form terminated cDNA fragments that are stochastically terminated upstream of the 3′UTR / poly(A) junction, but not within the poly(A) tail; isolating the terminated cDNA fragments; chemically ligating a functionalized 5′ adaptor to the terminated cDNA; and amplifying the chemically-ligated cDNA into an amplification product, wherein the cDNA is enriched for sequences at the 3′UTR / poly(A) tail junction without fragmentation or enzymatic ligation.

A method is provided for monitoring a cell culture in a culture medium in a container (3) by means of a computer (2) having software (5) wherein the container (3) is connected to at least one sensor (6) for measuring the cell culture (5), the software (5) uses the spectral data measured by the at least one sensor (6) to create and operate a data model (8) to calculate specific process parameters of the cell culture, in which first a synthetic cell-free culture medium sample is measured with the at least one sensor (6); comprising different ranges of culture medium compounds and / or metabolites and / or products and / or process parameter combinations, and then real-time monitoring of target cell culture parameters is performed using the data model (8) trained by the software (5).

Provided herein are compositions and methods comprising a synthetic cell that generates adenosine triphosphate (ATP) and / or nicotinamide adenine dinucleotide (NADH). In particular, the present invention relates to the compositions and methods comprising synthetic cells that generate ATP and / or NADH from the uptake of glucose.

The invention provides an assembling mode of RNA liquid drops deformed through photostimulation and application of the RNA liquid drops, and belongs to the technical field of preparation of nucleic acid for analysis. The method comprises the following steps: mixing an RNA single strand with a buffer solution through structural design to obtain a mixed system with a specific concentration; after the mixed system is subjected to heat shock to complete base pairing and assembling, sealing and packaging are performed, the RNA liquid drops deformed through photostimulation are obtained, and the diameter of the liquid drops is 7.0-13.0 microns. The RNA liquid drop obtained in the embodiment can deform under light stimulation, behavior regulation and control can be achieved through group modification, and feasibility is provided for construction and functional expression of artificial cells.

The invention discloses a preparation method of a bionic system based on a microfluidic technology, and relates to the technical field of functional bionic materials. Chitosan, nano-enzyme, alginate, phytic acid and the like are taken as raw materials, the artificial cell system with adjustable morphology, structure and activity is prepared in a high-throughput manner by utilizing a micro-fluidic chip through an intermolecular polymerization reaction, and the prepared artificial cell system can simulate a glucose metabolism function.