121 results about "Synthetic Cells" patented technology

Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

Determining the position of a mobile terminal operating in a packet-switched communications system based on timing advance values obtained through network initiated artificial cell hops. The mobile terminal may be instructed to perform a series of artificial cell changes so that timing advance values may be obtained for the mobile terminal with respect to a plurality of base stations. The position of the mobile terminal is then determined based on the timing advance values, optionally supplemented by signal strength measurements. The mobile terminal may contact a network entity via the base station in each cell before being instructed to change to the next base station. Or, the mobile terminal may be supplied with a list of base stations to contact, with the mobile terminal transmitting short access bursts to the base station in a given cell before automatically tuning to the next cell, without waiting for an acknowledgement.

In biology and biotechnology, electroporation is an important technique for introducing entities (DNA, RNAi, peptides, proteins, antibodies, genes, small molecules, nanoparticles, etc.) into cells. Applications range widely from genetic engineering to regenerative medicine to drug delivery. It has been demonstrated that the electrical currents flowing through cells can be used to monitor and control the process of electroporation for biological and artificial cells. In this application, a device and system are disclosed which allow precise monitoring and controlling electroporation of cells and cell layers, with examples shown using adherent cells grown on porous membranes.

An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.

Treatment for Cancer Using A Preparation containing a combination of one or more Natural or Synthetic or a Combination of Natural and Synthetic Boswellic Acids and one or more Natural or Synthetic or a Combination of Natural and Synthetic Cytokinins such as Kinetin, Kinetin Riboside and other Cytokinins and (the Preparation) could also contain one or more Natural or Synthetic or a Combination of Natural and Synthetic plant growth hormones such as Auxins in any percentage Ratio and could Optionally include Pharmaceutically Suitable Radioactive Agent (Radio Therapy) in any percentage ratio as a “Stand Alone” Treatment for Cancer or to Be used in Conjunction with other Pharmaceutically Suitable Therapy and Treatment(s) in any percentage ratio as a Treatment for Treating Cancer.

The invention discloses an amphiphilic porous hollow carbon microsphere, a preparation method thereof, applications of the amphiphilic porous hollow carbon microsphere as a carrier in enriching and loading of active molecules or a catalyst, and a method for separating and recovering the porous hollow carbon microsphere from a dispersion system by utilizing the amphipathy. The method comprises thefollowing steps of: dispersing microzyme in an aqueous solution of a cytoderm surface protectant; and then, carrying out carbonation treatment to obtain the amphiphilic porous hollow carbon microsphere. The prepared hollow carbon microsphere has a pore size capable of being controlled in the mesoporous and macroporous range as required, and can be suitable for the loading, the storage and the transmission of guest molecules in different sizes; by utilizing the characteristic of the amphiphilic porous hollow carbon microsphere capable of spontaneously transferring to a two-phase interface froma polar or non-polar dispersion system, the microsphere can be conveniently separated and recovered from a reaction mixing system. The easy and low-cost synthesis method and the special properties ofthe hollow carbon microsphere are favorable to the applications of the hollow carbon microsphere to the fields, such as energy storage, medicament transmission, artificial cells, catalytic carriers, and the like.

A composition and a method of producing a composition which simulates hydration, cleansing and other properties of native vernix. The composition contains hydrated synthetic cells in a lipid matrix to provide rheological properties which are substantially similar to those of native vernix, and may also contain proteins. In one embodiment, the composition contains cubosomes/water with up to 30% protein and about 5% lipid to about 30% lipid. The composition may be used to cleanse newborn skin, compromised skin surfaces, as well as normal skin, to provide hydration/barrier function, and other applications.

A method for the treatment of spider silk filament for use as a thread or composition in the manufacture of cosmetic, medical, textile, and industrial applications, wherein the spider silk filament, derived from genetically modified organisms, is treated with at least one component selected from the group consisting of vitamins, hormones, antioxidants, chelating agents, antibiotics, preserving agents, fragrances, dyes, pigments, magnetic nanoparticles, nanocrystals, cell adhesion enhancers, thermal insulators, shrinkage agents and cosmetic, medical or dermatological active substances. Textile fabrics obtained by this method are stronger, bio-compatible, bio-degradable and have a higher thermal conductivity. Treated spider silk filament can also be applied in an oil-in-water or water-in-oil protective cream that is hypoallergenic and ensures a firmer skin.

The invention discloses artificial cell membrane materials applied to photoinduction stem grafting and a synthesis method of the artificial cell membrane materials. The synthesis method of the artificial cell membrane materials comprises the following steps that 2-(dodecyl trithiocarbonate)-2- methyl propionic acid, dicyclohexyl carbodiimide, 4- dimethylamino pyridine and dichloromethane are added into a flask, all the components are stirred and dissolved under the protection of nitrogen, azide ethanol is dropwise added and stirred, washing is carried out through diluted hydrochloric acid and distilled water, solvents are removed through a reduced pressure distillation method, and a yellow oily liquid a is obtained; MPC, a midbody a, azo different nitrile and absolute ethyl alcohol are added into a boiling tube and are evenly dissolved, then after the nitrogen is removed through three times of 'vacuumizing-induction of highly pure nitorgen' operations on the boiling tube, and reaction away from light is carried out on the boiling tube in a sealed mode under the protection of highly pure nitrogen; the reaction liquid is precipitated through trichloromethane/diethyl ether mixed solvents, and solid products are collected. By means of the technical scheme, the artificial cell membrane materials have good biocompatibility, achieve the various effects of restraining of protein adsorption, anticoagulation, surface lubrication and the like, and have good medical prospects.

The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.

The present invention features a freestanding microfluidic pipette with both solution exchange capability and fluid re-circulation, enabling highly localized and contamination- free fluid delivery within a confined volume in the vicinity of the pipette exit. Preferably, the device features direct positioning, so the pipette can be directed at a point or object of interest, such as a biological or artificial cell, a defined surface area or a sensor element within an open volume, using micro- or nanopositioning techniques. One aspect of the invention provides a free-standing pipette. The free-standing pipette includes a microfluidic device comprising one or more channels with exits leading into an open-volume and a positioning device programmed to hold the microfluidic device at an angle from a horizontal axis.

The invention provides a preparation method of a liposome vesicle based on a microfluidic device. The preparation method comprises the steps of: preparing a microfluidic chip by a soft lithography method; preparing a precursor solution for preparing the liposome vesicle; and using a constant-pressure injection pump to inject the precursor solution into a ''Y''-shaped chip through different injection ports, setting a total flow rate, adjusting the flow ratio of a buffer solution to a phospholipid molecular alcohol solution, and collecting a product at an outlet, wherein the product is the prepared liposome vesicle. The microfluidic chip selected by the invention is a ''Y''-shaped microfluidic chip with a serpentine mixing channel of a square structure, and the chip is prepared by the soft lithography method. By using the chip, based on the principle of self-assembly on an interface, by adjusting the total flow rate and the flow rate ratio of the aqueous buffer solution to the phospholipid molecule ethanol solution, regulation of the size of the liposome vesicle and control on the dimensional uniformity are achieved, and an efficient low-cost method is provided for preparation of artificial cells with uniform properties as well as drug carriers.

Modified fusion enhanced erythrocytes (or other cell types and synthetic cells) including human viral receptor proteins, human viral coreceptor proteins and viral derived proteins capable of mediating entry of respective viruses into the modified erythrocytes, cells or pseudo-cells and the method of using the fusion enhanced modified erythrocytes, cells or pseudo-cells for the treatment or prevention of viral infections. The fusion enhanced modified erythrocytes comprises CD4 and at least one HIV coreceptor, such as CXCR4 or CCR5 and as well, at least one of cholesterol rafts, fusin, actin, a viral derived protein such as fusion peptide derived from HIV GP120 or HIV GP41 or a shorter protein derived from a long viral protein, such as a portion of HIV derived GP120, or HIV GP41 such as the 23 N-terminal peptide of the HIV-1 gp 41 protein (AVGIGALFLGFLGAAGSTMGARS) called FP23 (Fusion Peptide). These viral-fusion enhanced cells may also be electrostatic charge enhanced through further additions named in this invention. The modified erythrocytes, when administered to an HIV patient, bind to the plasma virus and induce the injection of the HIV ribonucleoprotein complex into the cells. The entrapped viral content is sequestered within said cell for at least the period of time that the cell maintains its outer membrane integrity. The virus is thereafter either degraded or deactivated within the erythrocytes, cells or pseudo-cells, or destroyed by erythrophagocytosis.

Intended for the use in neurosurgery and organ tissue engineering to replace defects of nervous tissue of a mammal brain and spinal cord (B&SC) in reconstructive neurosurgical operations, a tissue-replaceable artificial cell-biopolymer neuroendoprosthetic system (ACBP NEPS) for surgical plasty of defects of nervous tissue of B&SC, stimulating regeneration and growth of damaged axons of neural cells, comprises an elastic cell-biopolymer biologically active mass, produced from a heterogeneous collagen-containing matrix for implantation, and a biocomposition of cell preparations of various types of autologous cells of a patient. Also disclosed are a method of producing the ACBP NEPS, which provides for perfusion of the biocomposition of the cell preparations of various types of autologous cells of a patient into a heterogeneous collagen-containing matrix for implantation, and a method of reconstructive neurosurgical operation comprising implantation of the ACBP NEPS into a defect of neural tissue.

The invention provides a polypeptide nano-material containing double-pyrene groups as well as a preparation method and an application of polypeptide nano-material. The polypeptide nano-material containing the double-pyrene groups has a structure represented as a formula I; the polypeptide nano-material containing the double-pyrene groups can be deformed for self-assembly and can be self-assembled to form a nanofiber network structure in a tumor area and construct an artificial cell extracellular matrix in situ, and the artificial cell extracellular matrix selectively adheres to the tumor area to form a migration barrier, so that tumor invasion and metastasis can be effectively inhibited, and the polypeptide nano-material has board medical application prospect.

The invention relates to a preparation method of thylakoid-containing artificial cells and a photosynthesis simulating method, and belongs to the fields of biotechnology, biochemistry and synthetic biology. The method comprises the following steps of extracting thylakoid particles from fresh spinach leaves, and storing the thylakoid particles in a buffer solution for use; smearing a phospholipid solution onto an ITO glass electrode; after the solvent volatilization, forming a layer of phospholipid membrane; fixing a polytetrafluoroethylene framework by vacuum grease; adding liquid phases intothe polytetrafluoroethylene framework; applying alternating current onto the ITO glass electrode for preparing a GUV solution; mixing the GUV solution and a thylakoid solution; preparing the thylakoid-containing artificial cells. The thylakoid-containing artificial cells are prepared through the enlightenment of the natural plant cell structure and function; the model can realize the optical driveelectron transfer process under the artificial sunlight irradiation. Through the model building, the attributes of chloroplasts in nature are doubly simulated from the structure and the function. Thecomplicated cells realize simplified research, so that the development and the progress of the biomimetic chemistry are promoted.

The invention discloses a saccharomyces cerevisiae recombinant bacterium capable of producing alpha-humulene, 8-hydroxy-alpha-humulene and zerumbone and a construction method thereof. The method comprises the following steps: introducing an optimized alpha-humulene synthase encoding gene ZSS1, an optimized cytochrome P450 enzyme encoding gene CYP71BA1, an optimized cytochrome P450 reductase encoding gene AtCPR1 and an optimized dehydrogenase encoding gene ZSD1S114A into saccharomyces cerevisiae by utilizing homologous recombination; and obtaining a saccharomyces cerevisiae recombinant bacterium 1 capable of producing alpha-humulene, 8-hydroxy-alpha-humulene and zerumbone. Experiments prove that the saccharomyces cerevisiae recombinant bacterium capable of producing alpha-humulene, 8-hydroxy-alpha-humulene and zerumbone, constructed by the invention, can produce alpha-humulene, 8-hydroxy-alpha-humulene and zerumbone, and lays a foundation for synthesizing alpha-humulene, 8-hydroxy-alpha-humulene and zerumbone by artificial cells.

The invention discloses a method for constructing artificial cells based on Janus magnetic nanoparticles and a DNA directional immobilization technology, and belongs to the field of artificial cell preparation. The preparation method comprises the following steps: firstly, preparing single-stranded DNA modified Janus magnetic nanoparticles and complementary single-stranded DNA functionalized glucose oxidase and horseradish peroxidase; mixing the Janus particles with an enzyme, and realizing immobilization of the enzyme through DNA complementary hybridization; jointly incubating immobilized double enzymes and tannic acid, realizing encapsulation of the immobilized enzymes through self-polymerization of the tannic acid, and constructing the artificial cell with a compartment structure. The artificial cell prepared by the method is simple in preparation process, mild in condition, high in internal enzyme activity, easy to separate from a reaction system and excellent in stability, and canbe used for simulating cascade reaction in natural cells.