43 results about "Synthetic Cells" patented technology

The invention discloses a preparation method of an artificial cell coated with a macrophage membrane, and belongs to the technical field of artificial cell preparation. The preparation method comprises the following steps: dissolving poly (diallyldimethylammonium chloride) and sulfobutyl-beta-cyclodextrin in ultrapure water, and mixing an aqueous solution of the poly (diallyldimethylammonium chloride) and an aqueous solution of the sulfobutyl-beta-cyclodextrin to obtain a PDDA / SBE-beta-CD condensation liquid droplet solution; and mixing the macrophage membrane suspension and the PDDA / SBE-beta-CD condensed fluid drops in an equal mass ratio, and realizing in-situ coating of the macrophage membrane by adopting an ultrasonic coating technology to obtain the artificial cell coated with the macrophage membrane. The PDDA / SBE-beta-CD condensation liquid droplet constructed by the invention has good stability under the conditions of physiological salt concentration and pH and at 37 DEG C; the artificial cell coated with the macrophage membrane is prepared by mixing with the macrophage membrane suspension, and the macrophage membrane serving as a shell not only can prevent aggregate aggregation, but also can improve the structural stability and biocompatibility.

The application discloses a kind of artificial cells for single-cell mass spectrometry and preparation method thereof, wherein artificial cell includes internal water phase, intermediate oil phase and external water phase;Internal water phase includes polyethylene glycol and polyvinyl alcohol aqueous solution, intermediate oil phase includes L-alpha-phosphatidylcholine chloroform and hexane mixture;External water phase includes PVA and F-68 aqueous solution.Compared with natural cell sample, the novel artificial single cell based on microfluidic self-assembly developed in the application has better uniformity, stability and controllability, effectively avoids significant measurement difference between single-cell individual samples, and effectively solves the problem of difficult stable preservation of biological samples.The proposed artificial cell preparation method will largely solve the problem of lack of standard reference material in the field of single-cell mass spectrometry, making the results of single-cell mass spectrometry method more accurate, reliable and mutually recognized.

The invention discloses a multi-chamber vesicle as well as a preparation method and application thereof. Each multi-chamber vesicle is an anionic liposome, and a cavity of the anionic liposome contains a plurality of cationic liposomes and a buffer solution containing Mg < 2 + >. According to the preparation method of the multi-chamber vesicle, a special oil phase and a water phase with different charges are introduced, so that the multi-chamber vesicle in which a plurality of cationic liposomes are wrapped by a single anionic liposome is successfully prepared. The multi-chamber vesicle is good in biocompatibility, controllable in particle size and stable in mechanical property, and provides technical support for preparation of artificial cells and novel drug carriers in the future.

The utility model relates to the technical field of protein extraction, and discloses a protein extraction scraper device which comprises a base, a scraper assembly is arranged at the top of the base, the scraper assembly comprises fixing plates, a sliding groove, a rotating shaft, a first connecting rod, a second connecting rod, a connecting plate, a scraper mechanism, a guide rail and a sliding block, and the fixing plates are arranged on the two sides of the top of the base; sliding grooves are formed in the fixing plates, a rotating shaft is arranged between the two fixing plates, and first connecting rods are arranged at the two ends of the rotating shaft. According to the protein extraction scraper device, by arranging the scraper assembly, manual cell scraping can be replaced, traditional cell scraping is replaced, the labor intensity of workers is reduced, a driving motor is started to be matched with a rotating shaft, a first connecting rod, a second connecting rod and a connecting plate, and a scraper mechanism can be driven to move downwards; the stepping motor is started to drive the scraper blade to rotate, and bottom cells of the culture dish are scraped.

A synthetic cytokine signaling system includes an engineered cytokine signaling chain encoding a transmembrane polypeptide that contains a particular variant intracellular domain of IL9R and an extracellular domain of a cytokine receptor such as IL2Rβ, IL4Rα, IL7Rα, IL9R, and IL21R. Depending on whether a functionally compatible cytokine ligand domain is fused on the N-terminus, the engineered cytokine signaling chain may be in a self-activating form, or optionally may be in a non-self-activating form. In the latter case, the synthetic cytokine signaling system may further include an engineered cytokine ligand chain encoding a corresponding cytokine ligand that can be membrane-bound or secreted. Immune cells modified with the synthetic cytokine signaling system displays improved characteristics compared with unmodified immune cells, such as the zero or reduced dependency on IL-2 when cultured in vitro, enhanced activation upon antigen stimulation, increased cytotoxicity against target cells, and improved tumor control capacity when transferred in vivo.

The present invention relates to a device for water-in-air generation of droplets comprising a collection vessel, a droplet generation device positioned above the collection vessel comprising a piezoelectric transducer (21) and at least one capillary (22) with a nozzle (23) pointing downwards to the collection vessel; a laser source (30) generating a visible light laser beam (31) with tophat profile, and an imaging setup for continuously monitoring the droplet generation (40). Said device is particularly useful for preparing monodisperse giant unilamellar vesicles of any composition and three-dimensional microenvironment niches for cells or synthetic cells in high throughput.

A method is provided for monitoring a cell culture in a culture medium in a container (3) by means of a computer (2) having software (5) wherein the container (3) is connected to at least one sensor (6) for measuring the cell culture (5), the software (5) uses the spectral data measured by the at least one sensor (6) to create and operate a data model (8) to calculate specific process parameters of the cell culture, in which first a synthetic cell-free culture medium sample is measured with the at least one sensor (6); comprising different ranges of culture medium compounds and / or metabolites and / or products and / or process parameter combinations, and then real-time monitoring of target cell culture parameters is performed using the data model (8) trained by the software (5).

The invention provides an assembling mode of RNA liquid drops deformed through photostimulation and application of the RNA liquid drops, and belongs to the technical field of preparation of nucleic acid for analysis. The method comprises the following steps: mixing an RNA single strand with a buffer solution through structural design to obtain a mixed system with a specific concentration; after the mixed system is subjected to heat shock to complete base pairing and assembling, sealing and packaging are performed, the RNA liquid drops deformed through photostimulation are obtained, and the diameter of the liquid drops is 7.0-13.0 microns. The RNA liquid drop obtained in the embodiment can deform under light stimulation, behavior regulation and control can be achieved through group modification, and feasibility is provided for construction and functional expression of artificial cells.

The invention discloses a preparation method of a bionic system based on a microfluidic technology, and relates to the technical field of functional bionic materials. Chitosan, nano-enzyme, alginate, phytic acid and the like are taken as raw materials, the artificial cell system with adjustable morphology, structure and activity is prepared in a high-throughput manner by utilizing a micro-fluidic chip through an intermolecular polymerization reaction, and the prepared artificial cell system can simulate a glucose metabolism function.

The invention relates to a carbon nanotube embedded artificial cell, a preparation method thereof and an application of the carbon nanotube embedded artificial cell in degradation of antibiotic resistance genes, and belongs to the technical field of environmental functional materials. According to the material, an artificial cell membrane formed by lipidosome serves as a shell, layered double-oxide hollow spheres derived from ferrocobalt layered double hydroxides are packaged in the shell, and carbon nanotubes are embedded into the artificial cell membrane to serve as a transmission channel. The preparation method comprises the following steps: preparing carbon spheres by a hydrothermal method, preparing ferrocobalt layered double hydroxides by a coprecipitation method, carrying out composite calcination to obtain layered double oxide hollow spheres, and finally constructing artificial cells by a film hydration method and embedding the artificial cells into the carbon nanotubes. The material can be used for catalytically degrading antibiotic resistance genes in water, singlet oxygen and superoxide free radicals are generated through peroxymonosulfate activation, gene segments are effectively attacked, and efficient degradation is achieved. The method has the advantages of efficient catalytic activity and confinement encapsulation, magnetic recovery is facilitated, and a novel environment-friendly method is provided for treatment of antibiotic resistance genes.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and verified by this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate compound for cancer prevention or treatment.

The invention discloses an artificial cell and a preparation method thereof as well as a bionic tissue and a preparation method thereof, an aqueous dispersion of a negative electricity nanometer material is sprayed into an aqueous phase receiving bath containing a cationic polyelectrolyte and a surfactant through a gas-liquid shearing microfluidic technology, and one-step construction of an artificial cell membrane and a cytoskeleton is realized. According to the method, the internal skeleton enhanced artificial cell is constructed in a water phase by a one-step method by utilizing the electrostatic interaction of the negative electricity nano material and the cationic polyelectrolyte, and the method has the characteristics of convenience in preparation and excellent stability. By introducing different functional materials, multifunctional integration of the artificial cell can be realized. Meanwhile, the adhesion inducer is added, so that a bionic tissue can be further constructed, and the bionic tissue integrates excellent mechanical properties of the artificial cells and can bear disturbance to a certain degree without disintegration.

An AI platform is used to develop a combination therapy for a patient suffering from a tumor that has formed clones. The combination therapy, containing at least two disturbances, is capable of targeting clones (including subclones) that have evaded therapeutic intervention due to resistance and / or evolution. The AI ​​platform is trained on disturbance data obtained from at least one cell line that has similar properties to a relevant clone. The trained AI platform predicts how the relevant clone will respond to disturbances and ranks the disturbance responses from strongest to weakest. At least one cell line can be an existing cell line from an established database or a synthetic cell line generated by the AI ​​platform.The AI ​​platform can include one or more from a machine learning platform, a deep learning platform, an artificial neural network (ANN), a convolutional neural network (CNN), and a generative adversarial network (GAN).

The invention belongs to the technical field of functional nano materials and biomimetic catalysis, and discloses a universal preparation method and application of high-entropy nano enzyme. According to the method, various metal salts, polyvinylpyrrolidone, benzyl alcohol and formaldehyde are used as raw materials, and various high-entropy alloy nanoparticles and nanosheets are prepared under the same reaction condition through a solvothermal method. The prepared high-entropy nano-enzyme has the advantages of controllable composition, uniform morphology, high catalytic activity and the like, can simulate the activity of various natural enzymes, and is suitable for the fields of artificial cell systems, biological catalysis, sensing and the like.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and validated in this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate drug for cancer prevention or treatment.

The disclosure provides synthetic cytotoxic cells, comprising a hydrogel microsphere and a TNF-related apoptosis-inducing ligand (TRAIL), or active fragment thereof. In aspects, the TRAIL or active fragment thereof is attached to the surface of the hydrogel microsphere. The disclosure further provides methods of preparing the synthetic cytotoxic cells and methods of use thereof in the treatment of cancer.

Methods of synthesizing annotated cell microscopy images are provided, comprising: inputting a random noise vector into a deep learning model trained using real cell microscopy images and corresponding greyscale segmentation masks, to generate synthetic cell microscopy images and corresponding greyscale segmentation masks. Related methods, products and systems are also described.

Some embodiments provide for a method of using a trained machine learning model to predict a characteristic of a subject, the method comprising: processing cfRNA expression data using the trained machine learning model to obtain an output indicative of the characteristic of the subject, wherein the trained machine learning model was trained using artificial cfRNA expression data, the artificial cfRNA expression data comprising a plurality of artificial cfRNA expression profiles, an artificial cfRNA expression profile having been generated by: generating a healthy expression profile component; generating a tumor expression profile component; and generating the artificial cfRNA expression profile using the healthy expression profile component and the tumor expression profile component.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

Synthetic ionophores for the active carry and transport of free ions across biological membranes in vivo, comprising coordination complexes of molecules with polarized hexa-aqua and / or tetra-aqua syst

The present disclosure relates generally to new synthetic cellular receptors that bind engineered ligands and have selectable specificities and activities. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and / or for the treatment of various health conditions or diseases, such as cancers.

The invention discloses a device suitable for aerosol phase virus-receptor affinity and activity evaluation, an evaluation method and a data analysis method. The device comprises an aerosol generation unit, a bionic artificial cell array unit, an environment control and fixing assembly and an activity evaluation and collection unit. The method is characterized in that through a micro-nano processing and molecular self-assembly technology, a supporting lipid double-layer membrane is constructed on an array substrate, receptor protein is directionally fixed, and a bionic cell interface is formed. When in use, a virus sample is atomized into aerosol and flows through the interface, so that the virus is specifically combined with the receptor; affinity parameters in an aerosol environment can be obtained by combining quantitative detection with viruses and utilizing algorithm model fitting. Meanwhile, the system can directly collect an aerosol sample after action and inoculate cells for culture, so that synchronous verification of virus biological infection activity is realized. The device is easy and convenient to operate and high in universality, and a brand new tool is provided for studying the recognition and infection mechanism of viruses under the real aerosol transmission condition.

The present invention relates to a method for manufacturing a three-dimensional structure based on a block copolymer. The method comprises the steps of injecting a block copolymer (BCP) solution into each micro-well formed on the substrate and drying it to form a block copolymer layer, and applying a buffer to the block copolymer layer to hydrate the micro-well in three dimensions Forming the structure, after the three-dimensional structure is formed, injecting and curing a hydrogel solution around the three-dimensional structure may include the step of enhancing stability. In particular, the process of hydration by applying a buffer to the micro-well is performed while an electric field is applied. By controlling the concentration of the block copolymer (BCP) and the amplitude and frequency of the electric field, a three-dimensional artificial cell membrane having a desired size and shape, such as a spherical or ciliary shape and high stability (100% survival for 50 days) is manufactured can do. The present invention can be efficiently applied to various biological fields such as artificial cells, cell-mimicking biosensors, and bioreactors.

Methods and compositions for embedding structures which are channels, hemichannels, pores, or have other structurally defined openings inside of a synthetic cell are discussed. These synthetic cells can be a vesicle. These synthetic cells with embedded structures can be used for a variety of detection and screening methods.

An AI platform is used for developing a combination therapy for a patient afflicted with a tumor that has produced clones. The combination therapy, which includes at least two perturbations, is capable of targeting clones (including subclones) that have escaped therapeutic intervention due to resistance and / or evolution. The AI platform is trained with perturbation data obtained from at least one cell line that has similar characteristics to a clone of interest. The trained AI platform predicts how the clone of interest will respond to perturbations and ranks the perturbation responses from highest to lowest. At least one cell line may be an existing cell line from a well-established database or synthetic cell line generated by the AI platform. The AI platform may include one or more of a machine learning platform, deep learning platform, artificial neural network (ANN), convolution neural network (CNN), and generative adversarial network (GAN).

The invention discloses an artificial cell capable of transferring nano-scale particles and a preparation method and application thereof, the artificial cell comprises self-expanding oil drops and a pore-forming agent, and the artificial cell is formed through hydrolytic condensation, heterogeneous nucleation and ultraviolet polymerization, the self-expanding oil drops are inorganic precursors with rich siloxane bonds and polymerizable methacrylate bonds, and the pore-forming agent is a high-molecular compound capable of being selectively removed. The surface of the artificial cell is provided with a plurality of nano-scale micropores, so that a transfer channel can be provided for nano-scale particles loaded in the artificial cell, and a new thought is provided for constructing a more bionic artificial cell.

The invention discloses an artificial cell wall system based on a DNA logic gate and application of the artificial cell wall system, the artificial cell wall system comprises a probe 1 (Apt-S-T1) and a probe 2 (T2-S-Apt), Apt is a nucleic acid aptamer targeting a to-be-treated cell membrane receptor, S is a spacer region sequence, and T1 and T2 are a first toe end sequence and a second toe end sequence respectively; a linker; the hairpin comprises a hairpin body 1, a hairpin body 2 and a cross-linking agent, and the tail end of the hairpin body 1 or the tail end of the hairpin body 2 is modified with a hydrogel framework material. Artificial cell walls are constructed based on a DNA logic gate, the integrity of cell membranes is protected in the freezing process, and DMSO-free cryopreservation is achieved; and the resuscitated cell has a biological activation function, so that the resuscitated cell not only can survive, but also has a proliferation capacity superior to that of a fresh cell. Besides, the cell cryopreservation method based on the artificial cell wall system has universality, corresponding artificial cell wall systems can be customized according to different cell types, and cryopreservation requirements of various cell types are met.

The invention relates to the technical field of biological medicines, in particular to the field of cell therapy, and in particular relates to a method for synthesizing a T cell receptor antigen receptor (STAR). The STAR starts from a human source TCR constant region, the risk of reducing mismatch and receptor rejection is obtained after specifically combined sites are mutated, STAR molecules capable of being highly expressed on a membrane are obtained, and particularly, superior membrane expression better than that of a mouse source is shown after the constant region is mutated. And the combination of several modifications has higher upper membrane expression than single or two modifications, and shows a synergistic effect. The mismatch between the STAR molecule and an endogenous TCR chain is reduced, and the STAR molecule has high stability, high upper membrane expression level, can play a role for a long time, and has good clinical application prospects.

Synthetic cellular support systems in the form of engineered extracellular matrices are provided. The cellular support system may include a three-dimensional scaffold structure comprising at least one void. At least one suspended fibril spans across the at least one void in the three-dimensional scaffold structure. The suspended fibril comprises at least one extracellular matrix protein, such as fibronectin, and at least one glycan, such as a hyaluronic acid. The suspended fibril is capable of supporting cells and promoting three-dimensional cellular growth. In various aspects, a plurality of suspended fibrils may span the void to form a three-dimensional suspended fibrillar network.

The application discloses a preparation method of macrophage membrane coated artificial cells and belongs to the technical field of artificial cell preparation. Polydiallyldimethylammonium chloride and sulfobutyl-beta-cyclodextrin are dissolved in ultrapure water, and the aqueous solutions of the two are mixed to obtain a PDDA / SBE-beta-CD condensate droplet solution; equal-mass-ratio macrophage membrane suspension liquid and the PDDA / SBE-beta-CD condensate droplet solution are mixed in equal ratio, in-situ coating of the macrophage membrane is realized by using ultrasonic coating technology, and macrophage membrane coated artificial cells are obtained. The PDDA / SBE-beta-CD condensate droplet constructed by the application has good stability under physiological salt concentration, pH conditions and 37 DEG C; the macrophage membrane coated artificial cells are prepared by mixing the macrophage membrane suspension liquid, and the macrophage membrane as the shell can not only prevent the condensate from gathering, but also increase the structural stability and biocompatibility.