72 results about "Synthetic nucleic acid" patented technology

The present disclosure relates to methods for the production of a cell-based product based on using synthetic promoters or a cellular input-output unit thereof, configured for controlling the expression of at least one nucleic acid sequence of interest, upon sensing dynamic cellular- and / or environmental- state and / or condition, that leads to a desired phenotype of the cells. The present disclosure further provides cell-based products, in particular, animal cell-based meat products (ACBM).

The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.

The present application relates to the application of Klenow mutant fragments in the synthesis of nucleic acids, and belongs to the technical field of biological modification and synthesis.The present application provides the application of Klenow mutant fragments with an amino acid sequence as shown in SEQ ID NO.8 or SEQ ID NO.14 in the synthesis of nucleic acids.The mutant protein F762A expressed by the Klenow mutant fragments of the present application can efficiently synthesize deoxyribonucleic acid, ribonucleic acid, deoxyribonucleic acid analogs and ribonucleic acid analogs with different lengths of DNA templates and DNA / RNA primers at 37 DEG C, and has a wide synthesis range and universality.

The present invention relates to a synthetic nucleic acid molecule comprising 3'-UTR polynucleotide with improved translation efficiency and a vaccine composition comprising the same, and more particularly to a synthetic nucleic acid molecule comprising 3'-UTR with improved translation efficiency manufactured by inclusion of specific motif and a codon-optimized signal sequence and an antigen encoding sequence, and a vaccine composition comprising the same. The synthetic nucleic acid molecule according to the present invention comprises a 3'-UTR polynucleotide with improved translation efficiency, which can effectively induce the expression of an antigenic polypeptide, which is useful for vaccine development because it can be expected to increase immunogenicity as a vaccine.

Synthetic nucleic acids are described that can be used for astrocyte-directed expression of heterologous nucleotide sequences, as well as methods of using same for astrocyte-directed expression of such nucleotide sequences for the treatment of neurodegenerative diseases.

Synthetic nucleic acids encoding mini and microdystrophin genes comprising the membrane binding motifs or domains of the R10-R11-R12 region are provided. Also provided are vectors, host cells, and related methods of using the same to treat a subject suffering from Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD) or X-linked dilated cardiomyopathy (XLDC), or for ameliorating one or more adverse effects of DMD, BMD, or XLDC. Also provided are a fusion protein comprising a nNOS binding domain of dystrophin R16-R17 that is operably linked to a syntrophin PDZ domain and synthetic nucleic acids comprising the same that can be used to treat subjects with diseases characterized by loss of sarcolemmal neuronal nitric oxide synthase (nNOS) activity.

The invention provides methods for synthesizing a product DNA molecule of any DNA sequence from a universal library of overlapping oligonucleotides. The method involves combining a plurality of the overlapping oligonucleotides in a reaction pool, where the sequences of the plurality of oligonucleotides comprise at least a sub-sequence of the product DNA molecule. The method also involves annealing the plurality of oligonucleotides, performing a ligation step, and performing an amplification step to thereby synthesize a sub-sequence of the product DNA molecule. The invention can be used to synthesize a DNA molecule of any possible sequence from the universal library, which can be accomplished through a hierarchal assembly scheme. In one embodiment the universal library comprises fewer than 10,000 pre-manufactured oligonucleotides that can be synthesized into the any possible DNA sequence.

The present disclosure concerns phosphoramidite compounds useful for nucleic acid hybridization probes, the probes containing them, and related methods of use and preparation.

An object of the present invention is to provide a solid-phase carrier for nucleic acid synthesis, in which a nucleic acid and an adduct can be easily separated by HPLC in a case where the nucleic acid is synthesized using the solid-phase carrier for nucleic acid synthesis and the nucleic acid is cut out, a compound which serves as a linker of the solid-phase carrier, a compound which serves as a precursor of the linker, and a method for producing a nucleic acid using the solid-phase carrier. The compound according to the embodiment of the present invention is a compound represented by the following general formula (1).In the general formula (1), Ar represents a tri- to tetracyclic aromatic ring which may have a substituent, Z represents a hydrogen atom or a protective group eliminable by an acid, and R1 to R4 each independently represent a hydrogen atom, an alkyl group, or an alkoxy group.

Synthetic nucleic acids that can be used for astrocyte directed expression of heterologous nucleotide sequences are described. Also described are methods of using the same for the targeted expression of astrocytes of such nucleotide sequences for the treatment of neurodegenerative diseases.

The present invention relates to methods and compositions for preparing a sample enriched for single stranded nucleic acids. The single stranded nucleic acids find use as templates for synthesizing Xpandomer copies of the nucleic acids. The Xpandomer copies may be passed through a nanopore sensor to determine the nucleic acid sequence of the nucleic acids. The methods include asymmetric PCR, amplicon immobilization melt, exonuclease-mediated partial or complete digestion of one or both strand of an amplicon and linear amplification.

To provide a porous resin bead for suppressing a rise in back pressure in a reaction vessel and stably producing a nucleic acid without liquid feeding failure in a process of synthesizing a nucleic acid. A porous resin bead characterized by consisting essentially of a styrene-hydroxystyrene-divinylbenzene-phenyl methylene malononitrile copolymer.

This disclosure relates to synthetic nucleic acid molecules, such as synthetic mRNA molecules, containing chemical modifications that improve nucleic acid stability while simultaneously preserving the ability of the nucleic acid to be processed by endogenous translational machinery and to furnish protein product.

Introduced here is an approach to developing molecules and molecule groups via a simulated mutagenesis process that is performed as part of in silico experimentation. Due to its initiation of the simulation based on a known binding interface or predicted binding interface between two structures—whether biological or synthetic—with known sequences, the approach introduced here can accomplish linear iteration of sequences. This can be accomplished whether these sequences relate to proteins, biological amino acids, synthetic amino acids, biological nucleic acids, synthetic nucleic acids, unnatural variants thereof, or any other molecules with a three-dimensional (“3D”) structure to evaluate the thermodynamic binding and affinity of the interaction as individual nucleic acids, amino acids or individual units of a polymer are mutated one at a time.

Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.

The present invention relates to methods and compositions for preparing a sample enriched for single stranded nucleic acids. The single stranded nucleic acids find use as templates for synthesizing Xpandomer copies of the nucleic acids. The Xpandomer copies may be passed through a nanopore sensor to determine the nucleic acid sequence of the nucleic acids. The methods include asymmetric PCR, amplicon immobilization melt, exonuclease-mediated partial or complete digestion of one or both strand of an amplicon and linear amplification.

A novel ubiquitous chromatin opening element (UCOE) named SRF-UCOE and methods for its use are provided. Compositions including recombinant and synthetic SRF-UCOE nucleic acid molecules, DNA constructs and vectors comprising the SRF-UCOE nucleic acid molecules, host cells comprising the DNA constructs or vectors, and cell culture systems comprising such host cells. SRF-UCOE polynucleotide sequences can be used in DNA constructs or expression cassettes for transformation and expression in cells or organisms of interest. The compositions and methods provided are useful for increasing and / or maintaining expression of a gene of interest. Transgenic cells, tissues, and animals comprising a SRF-UCOE nucleotide sequence are also provided. Methods are provided for increasing and / or maintaining expression of a gene of interest and for treating a subject via gene therapy.

A synthetic nucleic acid expression system for production of a transcript of interest in a predefined cell-state is provided, the system comprising (a) a first nucleic acid sequence comprising a first promoter operably linked to a nucleic acid sequence encoding a first trans-spliceable pre-mRNA sequence comprising at least one exon encoding a 5′ fragment of said transcript of interest and a first RNA sequence required for spliceosome-dependent trans-splicing; and (b) a second nucleic acid sequence comprising a second promoter operably linked to a nucleic acid sequence encoding a second trans-spliceable pre-mRNA sequence comprising at least one exon encoding a 3′ fragment of said transcript of interest and a second RNA sequence required for spliceosome-dependent trans-splicing; wherein said first promoter and said second promoter are different and each one is specifically regulated by said predefined cell-state.

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

Disclosed herein are methods of diagnosing severity, aggressiveness, or prognosis of a solid cancer by determining if miR-155 is overexpressed. Also disclosed herein are methods of treating, preventing, decreasing, eliminating, and / or ameliorating a cancer by synthetic nucleic acids encoding administering inducible T-cell costimulatory ligand (ICOSL) and / or RELA, thereby upregulating ICOSL and MHC-I on cancerous cells to improve the efficacy of immune cell therapies and checkpoint inhibitors. Any of the disclosed methods can further be paired with administration of an miR-155 inhibitor (e.g., cobomarsen).

Provided are a T7 RNA polymerase mutant capable of not generating an immunogenic by-product and having high transcriptional activity, and the use thereof, belonging to the technical field of biology. The amino acid sequence of the T7 RNA polymerase mutant is as shown in SEQ ID NO: 2. The T7 RNA polymerase mutant which is constructed by transformation of an original T7 RNA polymerase generates much less immunogenic RNAs than wild-type T7 RNA polymerases during a transcription phase, thus simplifying nucleic acid production processes, reducing the content of immunogenic by-products generated during the nucleic acid production processes, and shortening the time for synthesizing nucleic acids. The T7 RNA polymerase mutant has wide application prospects in the fields of RNA vaccines and drugs, in-vitro transcription, gene editing, virus RNA probe detection, etc.

The application belongs to the technical field of inorganic materials and analysis, and particularly relates to a kind of nucleic acid aptamer modified polydopamine coated magnetic composite material MagG@PDA@Au@Aptamer and its preparation and application in selectively separating recombinant histidine tag protein.The material of the application is synthesized by solvothermal method one-step magnetic graphene oxide composite material MagG, then coated with polydopamine to obtain MagG@PDA, then Au is bonded to the surface of MagG@PDA material, and then 5 end modified thiol nucleic acid aptamer is connected to the surface of the material to synthesize nucleic acid aptamer modified polydopamine coated magnetic graphene oxide composite material MagG@PDA@Au@Aptamer.The preparation method of the material of the application is simple, the nucleic acid aptamer solid loading is high, and the recombinant histidine tag protein in bacterial lysate can be separated and enriched efficiently and selectively.

The present invention provides methods for synthesizing a product DNA molecule of any possible DNA sequence from a universal library of overlapping oligonucleotides. The method involves combining a plurality of the overlapping oligonucleotides in a reaction cell, wherein the sequence of the plurality of oligonucleotides comprises at least a sub-sequence of the product DNA molecule. The method also involves annealing the plurality of oligonucleotides, performing a ligation step, and performing an amplification step, thereby synthesizing a subsequence of the product DNA molecule. The invention can be used to synthesize DNA molecules of any possible sequence from the universal library, which can be accomplished by a hierarchical assembly scheme. In one embodiment, the universal library comprises less than 10,000 prefabricated oligonucleotides that can be synthesized into any possible DNA sequence.

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

The present invention relates to and provides polypeptide libraries, such as synthetic antibody libraries which are suitable for selection of fully feline antibodies. The invention also relates to synthetic nucleic acid sequences which encode individual or collections of feline antibodies, i.e. nucleic acid sequences encoding feline antibody libraries. Methods for generating and using such libraries are provided. In particular, the invention relates to the preparation of a library of fully feline antibodies by the use of pre-evaluated synthetic sequences to generate a library of fully feline antibodies having favorable biophysical properties.

The present disclosure provides a method of measuring the quantity of analyte molecules. In some aspects, the method comprises compartmentalizing a sample with binding molecules conjugated to synthetic nucleic acid molecules such that the interaction of the binding molecules with the analyte molecules brings the nucleic acid molecules into proximity. Proximity triggers reactions that result in an optical signal, such as fluorescence, in analyte-containing compartments which can be counted to determine the quantity of analyte present.

The invention relates to a tumor antigen composition and application thereof. The invention particularly relates to a tumor antigen combination. The tumor antigen combination consists of tumor antigens with amino acid sequences as shown in SEQ ID NO.1 to SEQ ID NO.40. The invention also relates to a preparation method of the tumor antigen combination. The invention also relates to a corresponding separated or synthesized nucleic acid molecule combination, an expression vector, a host cell pharmaceutical composition, and application of a tumor antigen combination or pharmaceutical composition. The tumor antigen composition has remarkable affinity and immunogenicity, and can remarkably promote the killing effect on tumor cells.

The invention provides methods for synthesizing a product DNA molecule of any possible DNA sequence from a universal library of overlapping oligonucleotides. The method involves combining a plurality of the overlapping oligonucleotides in a reaction pool, where the sequences of the plurality of oligonucleotides comprise at least a sub-sequence of the product DNA molecule. The method also involves annealing the plurality of oligonucleotides, performing a ligation step, a selective digestion step, and an amplification step to thereby synthesize a sub-sequence of the product DNA molecule, and a produce DNA molecule using hierarchical assembly. The invention can be used to synthesize a DNA molecule of any possible sequence from the universal library, which can be accomplished through a hierarchal assembly scheme.

Provided herein are methods, compositions and systems comprising synthetic nucleic acid molecules that enable inducible or conditional pri-miRNA processing, preferably in mammalian cells in vivo. Provided herein are synthetic nucleic acid molecules referred to as Orthogonal RNA Interference induced by Trigger RNA (ORIENTR) that switches between an inactive form and an active form upon interaction with one or more specific RNA-trigger molecules, which can be e.g., a synthetic RNA-trigger, or a disease-specific RNA signals, such as disease-specific mRNA, miRNA, or other cellular RNA products with sequences that characterize a disease state of a cell. The interaction between the RNA-trigger molecules and the ORIENTR is preferably mediated by hybridization, which exposes, facilitates the formation, and / or allows the formation of a correctly folded pri-miRNA scaffold substrate that can be processed by proteins of the RNAi pathway (such as Dicer), leading to RNAi-mediated repression of a target gene. Also provided herein are methods of using such ORIENTR molecules for the treatment or prevention of a disease in a subject, as well as detecting the presence or absence of a target RNA in a biological sample or in vivo.

The invention relates to an azobenzene quenching unit and a preparation method thereof, a solid-phase synthesis carrier and a preparation method thereof, and a nucleic acid probe. The azobenzene quenching unit has a structural general formula as follows: in the formula, R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R12 and R13 are independently hydrogen, methyl, ethyl, propyl, isopropyl, phenyl, biphenyl, 2-furyl, 2-thienyl, methoxyl, ethyoxyl, isopropoxy, nitro, nitroso or cyano, n is equal to 0-6, and m is equal to 2-12. The azobenzene quenching unit contains a polyfluorocarboxylic acid connecting structure, the azobenzene quenching unit and amino on the surface of a solid-phase synthesis carrier form an amido bond through carboxylic acid of the polyfluorocarboxylic acid connecting structure, and the azobenzene quenching unit is connected to the solid-phase synthesis carrier and used for synthesizing nucleic acid. The synthesis yield of the 3 '-azobenzene quenching agent labeled nucleic acid can be effectively improved.