142 results about "Amino acid synthesis" patented technology

The invention relates to a method for detecting free amino acids in tea by using the reversed-phase high-performance liquid chromatography. The method comprises the following steps: drying and grinding fresh tea, and adding boiling water for digestion; after supernatant liquor is collected, repeatedly extracting and merging the supernatant liquor; after the supernatant liquor is sucked and filtered, carrying out precolumn derivatization for a tea soup sample with an AccQ.Tag derivatization reagent; optimizing chromatographic separation and detection conditions with HPLC and AccQ.Tag C18 special amino acid analysis columns, and finally separating and detecting 19 kinds of free amino acids such as glutamine. The method can simultaneously determine the contents of 19 kinds of free amino acids such as glutamine in the tea, makes up the vacancy that glutamine in the tea cannot be quickly and accurately analyzed based on the high-performance liquid chromatography qualitatively and quantitatively at present, and provides a new idea for researching the synthesis path of amino acids in the tea.

The invention discloses a method for synthesizing laurel acyl amino acid sodium. The method comprises the steps that lauric acid and phosgene react by being catalyzed by organic aryl amide, and lauroyl chloride is prepared; lauroyl chloride and amino acid react in an alkaline solution, and lauroyl amino acid salt is prepared; extraction separation is carried out, a catalyst is recycled from an organic phase, a water phase is sequentially subjected to acidification, filter separation, sodium hydroxide neutralizing and cooling crystallization, and laurel acyl amino acid sodium is obtained. According to the method, organic aryl amide is mainly adopted as the catalyst for the reaction of lauric acid and phosgene, the lauric acid acylation reaction is thorough, no residue exists, acyl chloride products do not need layering, distilling and other purifying processes can be directly used for synthesizing laurel acyl amino acid sodium, and especially the catalyst can be recycled and cyclically utilized through a simple method. Compared with use of existing DMF and other fatty amine catalysts, the process steps are greatly simplified, the yield and quality of the product are improved, production cost is reduced, environmental protection is facilitated, and the requirements of industrial production are met.

The invention discloses a preparation method of a lithocarpus polystachyus rehd fermented health drink, which comprises the following steps that: (1) adding lithocarpus polystachyus rehd sweet tea leaves into water, heating, boiling, maintaining boiling for 10 to 20min, staying still so that the lithocarpus polystachyus rehd sweet tea leaves are precipitated, and taking the supernatant as sweet tea solution; (2) selecting yeast fermentation fluid, acetic acid bacteria fermentation fluid and lactobacillus fermentation fluid as fermentation broth; and (3) switching the fermentation fluid into the sweet tea solution, adding sugar, staying still and culturing for 48 to 72h at 30DEG C, and taking filtrate as the lithocarpus polystachyus rehd fermented health drink. The method takes the lithocarpus polystachyus rehd sweet tea as a culture medium to be fermented with lactobacillus, acetic acid bacteria and yeast, and the prepared lithocarpus polystachyus rehd fermented health drink has a plurality of health care functions and natural flavor. The lactobacillus, acetic acid bacteria and yeast that the method adopts can promote human development, improve the immunity and delay the body aging; and the lactobacillus has the effect of antibacterium, intestinal peristalsis promotion, digestion assistance, pathogenicbacteria killing, vitamin and amino acid synthesis in the intestinal tract and the like.

The invention provides ginkgo leaf freckle removing whitening cream. Raw materials of the ginkgo leaf freckle removing whitening cream comprise, by weight, 10-30 parts of ginkgo leaf, 10-20 parts of leonurus, 10-20 parts of radix angelicae, 10-20 parts of angelica sinensis, 10-20 parts of rhizoma bletillae, 10-20 parts of salvia miltiorrhiza, 10-20 parts of peach blossom, 10-20 parts of rhizome atractylodis, 10-20 parts of myrrh, 10-20 parts of calendula, 10-20 parts of Chinese trumpet creeper, 10-20 parts of scutellaria baicalensis, 10-20 parts of radix paeoniae alba, 10-20 parts of honey suckle, 10-20 parts of albizia flower, 10-20 parts of propollis, 10-20 parts of caulis spatholobi, 10-20 parts of mint and 10-20 parts of pearl. Ginkgol biloba extract has a good effect of removing radical and an antioxidation effect. Yellow glycoside, amino acid and amino acid synthesis collagen in ginkgo leaves have a large effect on beautifying, restraining melanin and maintaining skin gloss and elasticity, and the cream is suitable for long-term use and suitable for use at night.

The invention relates to a new antibiosis peptide and a preparation method and application thereof, which belongs to the field of antibiosis peptide and preparation. According to the invention, the temporin-1CEa gene of the new antibiosis peptide is cloned from the total RNA of the skin of rana temporaria chensinensis; and all the DNA sequences are shown in a sequence list. The No.144 to No.192 nucleotides in the gene are encoded as temporin-1CEa; the total sequence is Phe Val Asp Leu Lys Lys Ile Ala Asn Ile Ile Asn Ser Ile Phe-AMIDATION; and the molecular weight is 1733 Daltons. The antibiosis peptide temporin-1CEa which can be prepared through an amino acid synthesizer or a genetic engineering method has remarkable antibiosis and anticancer activity and no haematolysis on the erythrocytes of a mammal, and can be used for preparing antibiosis and anticancer medicines.

The invention relates to a solid-phase synthesis method of an antibacterial peptide Iseganan containing two pairs of disulfide bonds, which comprises the following steps: (1) according to a solid-phase synthesis method, sequentially coupling an amino resin used as a raw material with amino acids to synthesize a protected 17 peptide resin; (2) removing weak acid sensitive protecting groups, and then oxidizing to generate a first pair of disulfide bonds; (3) using iodine to remove Acm protecting groups, and cyclizing to generate a second pair of disulfide bonds; and (4) finally cracking from the resin, removing the protecting groups, and purifying to obtain the Iseganan antibacterial peptide. The route provided by the invention has the advantages of short operation steps, simple post treatment, fewer byproducts, high yield and low pollution, and is suitable for large-scale industrial production.

A hair treatment composition containing at least one peptide identical to human hair, where preferably the peptide is synthesized from naturally-derived amino acids and can serve as a natural alternative to the commonly used human or animal derived (wool) keratin peptides.

The invention relates to an alkyl tetramine synthesis method, an alkyl tetramine product and a shale inhibitor thereof. The synthesis method comprises the following steps of S1, amino acid synthesis; S2, esterification reaction; S3, ammonia transesterification; S4, Hofmann degradation reaction; S5, BOC removal protection. The alkyl tetramine product prepared by the method has the molecular structural formula shown as the accompanying drawing; the alkyl tetramine product and clear water are prepared into the shale inhibitor according to the proportion of 1 to 3 percent. The synthesis method provided by the invention is reliable; the synthesis yield is high; the raw material price is low; the production cost is low; the product is safe, achieves the environment-friendly effects, and is suitable for large-scale industrial production. The alkyl tetramine product is nontoxic and harmless and has good water solubility; the inhibition performance is obviously improved through being compared with that of similar products; meanwhile, the temperature-resistant capability reaches 240 DEG C; the product is particularly suitable for ultra-high-temperature deep well drilling.

The invention relates to a composition with a function of relieving phytotoxicity of a glyphosate herbicide and application of the composition, belongs to the field of treatment and prevention of crop diseases, and aims at providing a composition with a function of relieving phytotoxicity of a glyphosate herbicide. The active components of the composition comprise algal polysaccharide, brassin and gibberellic acid according to a weight ratio of 1 to (0.02-0.2) to (0.02-20). The composition is capable of relieving phytotoxicity of the glyphosate herbicide; the amino acid synthesis in plant bodies can be accelerated; the amino acids damaged by the phytotoxicity can be supplemented; the adsorption amount of the herbicide in the plant bodies can be reduced; meanwhile, the content of chlorophyll in the plant bodies can be increased; the growth of the crops can be quickly recovered; the influence of on the final yield of the crops caused by the phytotoxicity is reduced.

The present invention provides a method for the synthesis of β²-amino acids. The method also provides methods yielding α-substituted β-amino aldehydes and β-substituted γ-amino alcohols. The present method according to this invention allows for increased yield and easier purification using minimal chromatography or crystallization. The methods described herein are based on an aldehyde aminomethylation which involves a Mannich reaction between an aldehyde and a formaldehyde-derived N,O-acetal (iminium precursor) and a catalyst, such as, for example, L-proline or a pyrrolidine. The invention allows for large scale, commercial preparation of β²-amino acids.

The invention relates to a lipoid cation function molecule synthesized from natural cholesterol and amino acid and a preparation method and application thereof. The lipoid cation organic function molecule has hydrophobicity and hydrophilicity; a hydrophobic building block comes from rich natural lipoid compound cholesterol; a hydrophilic cation building block comes from natural amino acids having high biocompatibility and derivatives thereof; and a synthesis method is simple and efficient, and is easy for realizing large-scale preparation. Compared with a commercially available gene transfection reagent Bpe1-25k, the invention has the advantages that: the lipoid cation function molecule has remarkably low cell toxicity, can be taken as a gene vector for realizing efficient gene transfection in various cells, can be taken as a novel synthesis lipoid biological vector functional material for applying to loading and in-vivo transmission of genes and medicaments, and can be taken as a low-toxicity efficient cationic surfactant for applying to preparation and production of water aqua or emulsion cosmetics and personal nursing materials.

The invention discloses a synthetic method for teduglutide and belongs to the field of polypeptide drug synthesis. The method comprises the following steps of by taking amino acids at 2-3 positions ofa teduglutide sequence as pseudo-proline-dipeptide, or amino acids at 5-6 positions of the teduglutide sequence as pseudo-proline-dipeptide, or amino acids at 22-23 positions of the teduglutide sequence as pseudo-proline-dipeptide, or amino acids at 27-28 positions of the teduglutide sequence as pseudo-proline-dipeptide or amino acids at 29-30 positions of the teduglutide sequence as pseudo-proline-dipeptide, sequentially performing solid-phase synthesis on amino acids at other positions of the teduglutide sequence according to a sequence, synthesizing full-protection teduglutide, and performing cracking precipitation to obtain the teduglutide. The method provided by the invention improves the purity of crude peptide, greatly lowers the material cost and the purification cost and is beneficial to industrial amplification production.

The invention discloses a 1'-thio-aza indirubin compound, application and a preparation method thereof, belonging to the field of medical technology. The preparation method of the compound comprises the steps of: respectively condensing 5-substituted, 6-substituted, 7-substituted isatin derivatives or 1-alkyl-7-azaindole-2,3-dione with thieno-(2,3-b)-pyridine-3-ketone or benzo-3(2H)-ketone to obtain a 1'-thio-aza indirubin compound in which R=O, wherein sodium carbonate is used as a catalyst; and reacting the 1'-thio-aza indirubin compound in which R=O with hydroxylamine to obtain a 1'-thio-aza indirubin compound in which R=NOH. The invention also relates to the application of the compound in the preparation of antineoplastic agents, acceptable salts in pharmacy synthesized by the compound with inorganic acid, organic acid or amino acid. The compound of the invention can suppress or kill tumor cells, have the character of anti-tumor activity and can be used for preparing medicines for treating tumors.

The invention relates to an amino acid bound with polyethylene glycol with the following general structure: R-PEG-CO-X-amino acid or R-PEG-X-amino acid; wherein, PEG represents for polyethylene glycol chain; R represents for a hydroxyl group or an oxyl; the oxyl can be chosen from the C1 to C12 alkoxy, cycloalkoxy or aralkoxy; wherein, X represents for the heteroatom with active hydrogen removed except Alpha-carboxyl and Alpha-amino group in amino acid. Utilizing the amino acid bound with polyethylene glycol to synthesis polypeptide, the polypeptide is made from polypeptide positioned on specific position, not only improving the pharmacokinetics, but also having no effect of original treatment. The invention discloses the production method for the amino acid bound with polyethylene glycol.

Disclosed is a method for the combined synthesis of at least two vinylic monomers, at least one of which being an acrylic compound, comprising subjecting a monoconjugated alkene-1-carboxylic compound to reaction with a C₂-C₄ alkene under conditions of olefin cross-metathesis. The invention is particularly useful for extracting value from protein side streams. Upon protein hydrolysis, suitable amino acids (preferably phenylalanine or tyrosine) are subjected to reductive amination so as to form the corresponding alkene-1-carboxylic acid. Preferably after esterification and separation, this is used in cross-metathesis for the concomitant production of styrene resp. hydroxy styrene, and acrylates. The invention is applicable more widely, to the synthesis of olefins on the basis of carbohydrates, naturally occurring phenolic components, natural protein resources, or amino acids obtained from fermentations.

Amino acid connected with polyethylene glycol is provided with the following structural formula of R-PEG-X-amino acid, wherein the PEG is a polyethylene glycol chain, the R is hydroxyl or alkoxy of the PEG, the alkoxy can be selected from C1-C12 alkoxy, cyclo alkoxy or aralkyl alkoxy, and the X is a heteroatom without activated hydrogen except for alpha-carboxyl and alpha-amino in the amino acid. When the amino acid connected with the polyethylene glycol is used for synthesizing polypeptides, the polypeptides of the polyethylene glycol chain in a specific position can be obtained, and the polypeptides improve pharmacokinetics and do not influence original therapeutic effects of the polypeptides. The invention further discloses a preparation method for the amino acid.

The embodiment of the invention discloses an amino acid gold nanoparticle and a preparation method and application thereof. The amino acid gold nanoparticle is synthesized by a gold nanoparticle and amino acid. The embodiment of the invention further discloses a corresponding preparation method and application of the amino acid gold nanoparticle. The amino acid gold nanoparticle is beneficial to achievement of detection of the concentration of heavy metal ions in a living body.

The invention relates to SpyTag/SpyCatcher-cyclized L-beta-hydroxy-alpha-amino acid synthetase and an application thereof.

The invention relates to the technical field of chemical synthesis equipment, in particular to compound amino acid synthesis equipment which comprises a tank body and a sealing cover detachably fixed to an opening in the upper end of the tank body, the lower end of the outer surface of the tank body is fixedly connected with a supporting frame, and the center of the lower end of the tank body is fixedly connected with a discharging opening. A feeding port is fixedly connected to the upper end of the rear side of the outer surface of the tank body, a motor is fixedly connected to the center of the upper end face of a sealing cover, an output shaft of the motor penetrates through the sealing cover, extends into the tank body and is rotationally connected with the sealing cover, a connecting piece is fixedly connected to the lower end of the output shaft of the motor, and a stirring rod is arranged at the lower end of the connecting piece. The connecting piece is fixedly connected with the stirring rod through a screw. By designing a cleaning assembly, the outer surface of the stirring rod can be scraped and cleaned through the cleaning assembly after amino acid liquid in the tank body is discharged, and then attached residual corrosion of amino acid residual liquid is reduced, so that the service life of the stirring rod is prolonged, and the quality safety of amino acid processed products is improved.

The invention relates to a group of bemisia tabaci prevention and control target genes and combined application thereof. The group of bemisia tabaci prevention and control target genes comprise a BtGlu gene as shown in SEQ ID NO.1, a BtArgH gene as shown in SEQ ID NO.2 and a BtTY gene as shown in SEQ ID NO.3. The research thought of taking a sugar transporter gene, a basic group and a amino acid synthetic gene as the bemisia tabaci prevention and control target genes is provided for the first time; and experiments of the survival rate of the adult bemisia tabaci, the egg laying rate of the female bemisia tabaci, the influence on symbiotic bacteria of the female bemisia tabaci, the influence on the virus acquiring capacity of the female bemisia tabaci TYLCV and the like verify that the silent expression can obtain a short-term prevention and control effect-reduction of the survival rate of the bemisia tabaci and a long-term prevention and control effect-reduction of the reproductive capacity of the bemisia tabaci.