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Microbe additive for improving beach saline alkali soil structure and its preparation method and use

A technology of microbial addition and saline-alkali soil, applied in the field of microorganisms, can solve the problems of toxic degradation products of refractory degradation and soil compaction, and achieve the effect of sustainable development

Active Publication Date: 2015-07-08
江苏农丰宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemically synthesize macromolecular substances, some substances are difficult to degrade or the degradation products are toxic, and some macromolecular substances are easy to cause soil compaction

Method used

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  • Microbe additive for improving beach saline alkali soil structure and its preparation method and use
  • Microbe additive for improving beach saline alkali soil structure and its preparation method and use
  • Microbe additive for improving beach saline alkali soil structure and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of microbial matrices.

[0031] (1) Bacteria production

[0032] 1) Inoculate B. flavum IAE into liquid seed medium for cultivation, the cultivation conditions are: cultivation temperature 37°C, shaker rotation speed 150rpm, cultivation time 24 hours, seed bacteria content greater than 1×10 9 cfu ml -1 , the seeds are inoculated into the fermenter according to the proportion of 5% for liquid fermentation production. The conditions for the fermentation production are: the culture temperature is 30°C, the dissolved oxygen ventilation range is 30-100%, 150-220rpm, and the colony of the strain in the fermentation liquid in the late stage of fermentation Forming unit ≥ 1×10 9 cfu ml -1 . The formula of the liquid seed medium used is prepared according to 1L medium: 3g of beef extract, 10g of peptone, 3g of NaCl, 1000ml of tap water, pH range of 7.2, sterilized at 121°C for 20min; the formula of the liquid fermentation medium used is, according to 1...

Embodiment 2

[0037] Example 2 Preparation of microbial matrices.

[0038] (1) Bacteria production

[0039] 1) Inoculate B. flavum IAE into liquid seed medium for cultivation, the cultivation conditions are: cultivation temperature 37°C, shaker rotation speed 150rpm, cultivation time 24 hours, seed bacteria content greater than 1×10 9 cfu ml -1 , the seeds are inoculated into the fermenter according to the proportion of 5% for liquid fermentation production. The conditions for the fermentation production are: the culture temperature is 30°C, the dissolved oxygen ventilation range is 30-100%, 150-220rpm, and the colony of the strain in the fermentation liquid in the late stage of fermentation Forming unit ≥ 1×10 9 cfu ml -1 . The formula of the liquid seed medium used is prepared according to 1L medium: 3g of beef extract, 10g of peptone, 3g of NaCl, 1000ml of tap water, pH range of 7.2, sterilized at 121°C for 20min; the formula of the liquid fermentation medium used is, according to 1...

Embodiment 3

[0044] Example 3 Pot experiment of improving saline-alkali land in tidal flats with microbial matrix.

[0045] Pot experiments were used to determine the improvement effect of microbial matrix on tidal flat saline-alkali soil. The cotton variety is "Zhongmiansuo 35", which is a salt-tolerant variety. The tidal flat soil has been reclamated for 5 years. There are basically no green plants on the soil. The soil salt content is 13‰, pH 8.5, conductivity 5.33μS cm -1 .

[0046] Three treatments were set up in the experiment, namely:

[0047] Control group: saline-alkali soil without adding any substances;

[0048] Vinegar grains compost group: add 20% vinegar grains compost to saline-alkali soil;

[0049] Biological matrix group (the microbial matrix prepared in Example 1): the saline-alkali soil is added with a mass ratio of 20% microbial matrix;

[0050] The experimental pot (diameter 35cm, height 18cm) was put into 5kg saline-alkali soil or saline-alkali soil mixed with vi...

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Abstract

The invention discloses a microbe additive for improving a beach saline alkali soil structure and its preparation method and use. The microbe additive is prepared by combined fermentation of bacillus amyloliquefaciens IAE with an extracellular polypeptide gamma-polyglutamic acid secretion function and brevibacterium flavum IAE with an amino acid synthesis function. A fermentation substrate used for fermentation comprises, by weight, 900-930 parts of vinegar residue, 50-60 parts of (NH4)2SO4, 5-8 parts of K2HPO4, 5-8 parts of MnSO4 and 5-8 parts of MgSO4. The invention discloses a microbe matrix containing the microbe additive. The microbe matrix has an active bacterial strain amount of more than 1.0*10<8> cfu.g<-1>, effective improved substance gamma-polyglutamic acid content of greater than or equal to 20g.kg<-1> by dry weight, water content of less than or equal to 30% and organic matter content less than or equal to 45%. The microbe matrix can effectively improve beach saline alkali soil, has high efficiency and environmental friendliness and is conducive to sustainable development of beach soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial additive for improving saline-alkali soil structure of tidal flats, a preparation method and application thereof. Background technique [0002] my country has a continental coastline of 18,000 kilometers. A large amount of tidal flat land is formed due to sediment deposition. The comprehensive development of high-quality tidal flat soil can bring huge economic and ecological benefits to the society. Among them, the development of tidal flat soil agricultural land can provide The society provides a large amount of arable land resources. [0003] The tidal flat soil is mostly tidal salt sandy soil, and the soil salinity is mainly chloride sodium salt, with high salinity and alkalization degree. The soil basically does not contain coarse sand larger than 0.25mm, and the silt content is generally >50%. no structure. Soil silt particles are hardened ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/00C05G1/00
Inventor 陈立华邵孝侯金秋温扶辉缪其松沈珊珊
Owner 江苏农丰宝生物科技有限公司
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