57 results about "Rana" patented technology

The present invention relates to immunotoxins, that effectively kill malignant cells having a given surface marker and nucleic acid constructs encoding them. These reagents comprise a toxic moiety that is derived from a Rana pipiens protein having ribonucleolytic activity linked to an antibody capable of specific binding with a chosen tumor cell.

The invention discloses a method for extracting RNA of sweet potato root tuber and application thereof. Beta-mercaptoethanol and polyvinylpyrrolidone are used to prevent polyphenols from interfering the separation of RNA; and in addition, the NaCl with high concentration, absolute ethyl alcohol and potassium acetate are used, so the polysaccharose can be rapidly precipitated, and the influence of the polysaccharose on the extraction of RNA can be prevented. When preventing the interference effect of the polyphenols and the polysaccharose on the separation of RNA, RANA can still be remained in the solution, so the RNA with good quality, high purity and good completeness can be obtained, and the attainment rate and the yield of the RNA are large. The method has high practical application value, and can satisfy the research of molecular biology such as reverse transcription, Northern hybridization, cDNA library construction, transcriptome analysis and the like.

A defatted frog ovum powder as female health-care food for preventing osteoporosis in female menopausal phase and treating climacteric syndrome is prepared from defatted frog ovum powder, soybean extract, calcium, VC and additive.

The invention relates to a zymolysis method for extracting an oligopeptide mixture with high F value by Rana temporaria eggs. The method has the steps as follows: (1) after are compressing dried Rana temporaria eggs, and egg oil is filtered to obtain egg residue for standby; (2) the egg residue is placed in a reaction kettle and purified water is added, stirred and heated to 48 DEG C to 52 DEG C; and then after pH value is regulated to 8.0 to 8.5, peptide compound enzyme with the weight to be 1 percent to 3 percent of that of the egg residue is added for hydrolysis for 4h to 6h; the temperature is heated up to 80 DEG C to 85 DEG C, and insulated for 10min to 20min; and filtering is carried out to obtain filtrate which is frozen, dried and shattered to obtain the oligopeptide mixture with high F value. Or step (2) can be as follows: pH value is regulated to 1.5 to 2.5, pepsin with the weight to be 1 percent to 3 percent of the egg residue is added for hydrolysis for 4h to 6h; and after the pH value is regulated to 6 to 7, actin enzyme with the weight to be 0.5 percent to 1.5 percent of that of the egg residue is added for hydrolysis for 5h to 7h; and then the temperature is raised to 85 DEG C to 95 DEG C till finally the oligopeptide mixture with high F value is obtained. The method is characterized by reasonable technique, high yield and recyclable egg residue waste.

The present invention provides a lygodium japonicum (thunb.) sw lead-removing health beverage and a preparation method thereof, wherein the raw material components comprise, by weight, 5-8 parts of ginseng, 3-4 parts of milkvetch root, 4-5 parts of lotus seeds, 4-5 parts of longan, 4-5 parts of lily, 6-8 parts of lygodium japonicum (thunb.) sw, 1-2 parts of silene conoidea l, 10-15 parts of beta vulgaris leaf, 3-4 parts of celery leaf, 3-4 parts of white radish leaf, 3-4 parts of lettuce leaf, 0.1-0.2 part of rana temporaria oil, 4-6 parts of a nutritional additive, 0.1-0.3 part of citric acid, 5-8 parts of honey, 2-3 parts of xylitol, 0.1-0.3 part of a lotus leaf extract, and 800-1000 parts of water. The lygodium japonicum (thunb.) sw lead-removing health beverage has a lead-removing effect, has a sweet and sour taste, is particularly suitable for long-term use of high blood lead people, and has promotion application values.

The invention discloses an environment-friendly type feed additive capable of preventing skeletal muscle edema of Rana catesbeiana Shaw. The feed additive comprises sodium selenite, vitamin E, vitamin C, folium isatidis and astragalus membranaceus. The environment-friendly type feed additive has a useful effect of promoting growth of the Rana catesbeiana Shaw, has a remarkable effect of preventing the skeletal muscle edema of legs of the Rana catesbeiana Shaw, has a useful effect of treating the skeletal muscle edema of the Rana catesbeiana Shaw fed with changed feeds and is capable of lowering the probability of inducing the death of the Rana catesbeiana Shaw by the skeletal muscle edema of legs.

The invention relates to an antibacterial lotion containing rana antibacterial peptides and a preparation process thereof. The rana antibacterial peptides are dissolved in a phosphate buffer with the pH of between 5 and 7 and are added with a mannitol protecting agent to prepare a light yellow transparent liquid, namely the antibacterial lotion. The lotion has the functions of sterilization, disinfection, itching relief, astringency and hemostasis, fever removal and insecticide, and the like, is non-toxic and harmless to a human body, does not irritate skin and mucous membrane, can be widely applied to sanitation and cleanness of human skin and mucous membrane, antibacterial and sterilization and other fields with sterilizing effect reaching 100 percent. Furthermore, due to the addition of the mannitol protecting agent, the duration of effective antibiotic activity of the antibacterial lotion is greatly prolonged.

Disclosed is an pharmacy application of both a serine protease inhibitor of Rana grahami Graham's frog and a gene thereof, pertaining to biomedicine field. Serine protease inhibitor of Rana grahami Graham`s frog is a cyclic polypeptide encoded by a gene of Rana grahami Graham's frog, which is a Chinese amphibian. Said serine protease inhibitor has a molecular weight of 1951.38 dolton, an isoelectric point of 9.51, and no enzymatic activity. The complete sequence thereof is NH2-AVNIPFKVHFRCKAAFC-COOH,in which Cys at 12th site and Cys at 17th site form an intramolecularly disulfide bond. The coding gene is composed of 301 nucleotides, and the mature coding part is nucleotides from 160th site to 213th site. Artificial serine protease inhibitor of Rana grahami Graham's frog has strong suppression activity for serine protease, and can be used in pharmacy for preparing medicine for tumor, gastritis, and pancreatitis. Said serine protease inhibitor has a simple sequence and can be synthesized with ease.

In order to solve the identification problem of China rana rugulosa and Thailand rana rugulosa which have exactly-like appearance during actual protection of China rana rugulosa, the invention provides a molecular identification method for China rana rugulosa and Thailand rana rugulosa. The identification method is based on PCR technology, helps to realize rapid identification on China rana rugulosa and Thailand rana rugulosa by designing a specific primer for amplification, and specifically comprises the steps: 1) extracting DNA of a sample; 2) detecting sample DNA; 3) performing PCR amplification by employing four pairs of specific primers; and 4) performing identification on the specific amplified product. According to the method, by designing the four pairs of primers related to different gene positions, China rana rugulosa and Thailand rana rugulosa can be identified by PCR amplification, the primers are strong in specificity, the agarose gel electrophoresis characteristic spectral band is high in stability and has no regional difference, and the method can help to obtain reliable beneficial effect during practical application.

The invention discloses a PCR (Polymerase Chain Reaction) rapid assay kit for rana boulenger guenther-infecting staphylococcus aureus and an assay method. The PCR rapid assay kit comprises a PCR assay kit for assaying the staphylococcus aureus of ana boulenger guenthers, which includes 2X mixed reaction buffer, sense primer S-F:5minute -AGGTAACGGCGTAAATAG-3minute, antisense primer S-R: 5minute-ACTTGCTTCAGGACCATA-3minute, Taq enzyme and sterilized ddH2O. The PCR rapid assay kit disclosed by the invention has the advantages of rapidness, accuracy and specificity in the assay of the rana boulenger guenther-infecting staphylococcus aureus, meets the requirement of clinical diagnosis and provides convenient conditions for the assay of the staphylococcus aureus infecting the rana boulenger guenthers.

The invention discloses a non-finger frog skin peptide and gene and application in the drug in the biological medical domain, which codes a single-chained peptide with molecular weight at 3355.95 and isoelectric point at 10.14, wherein the primary structure of peptide is Gly Leu Gly Gly Ala Lys Lys Asn Phe Ile Ile Ala Ala Asn Lys Thr Ala Pro Gln Ser Val Lys Lys Thr Phe Ser Cys Lys Leu Tyr Asn Gly; the gene of secretory peptide is composed of 731 nucleic acids, which codes 520-616th nucleic acid; the peptide possesses obvious bacteria and fungi inhibiting action, which can be applied to make microbe infection disease drug.

The invention relates to the field of agricultural breeding, and discloses an integrated overwintering breeding method for hatching and breeding rana chinensis in the same pond. The method comprises the steps of (1) greenhouse construction, (2) hatching pond construction, (3) construction of an anti-escaping fence area and a rana chinensis feeding activity area, (4) spawn hatching and froglet breeding, (5) overwintering management, (6) management in early spring and (7) management of a second breeding period. According to the method, the management is convenient, the labor is saved, and each person can manage ten greenhouses; the survival rate of froglets reaches 90% or above in the overwintering process; the rana chinensis can grow quickly, the breeding period is short, from spawn hatching to a fattening period, the rana chinensis experiences overwintering once, in autumn of the next year, adult rana chinensis can be sold, and the economic benefit is high.

The invention discloses a non-finger frog peptide and gene and application in the drug in the biological medical domain, which codes a single-chained peptide with molecular weight at 1814.11 and isoelectric point at 8.08, wherein the primary structure of peptide is Gly Cys Ser Arg Trp Ile Ile Gly Ile His Gly Gln Ile Cys Arg Asp (GCSRWIIGIHGQICRD); the gene of secretory peptide is composed of 312 nucleic acids, which codes 141-189th nucleic acid; the peptide possesses obvious bacteria and fungi inhibiting action, which can be applied to make microbe infection disease drug.

The invention relates to a staged feeding and female mutagenesis multifunctional feed of rana tadpole for reducing water body pollution. The feed is characterized in that: according to an adopted technical formula, the feed consists of a main material and auxiliary materials; and formulas of per kilogram of feed at different stages are different. The feed has the advantages that: a produced tadpole bait is in a frozen state at normal temperature, and is difficult to dissolve in water when fed; bait waste is reduced; the pollution of bait residues to a water body of a culture pond is avoided; the feed is similar to rana egg jelly and has prominent palatability; nutrients are proportioned according to different growth stages, and the feed has comprehensive and rich nutrition; the feed has obvious effects of preventing, controlling and treating the common diseases of the tadpole when a Chinese herbal medicine compound preparation for preventing the diseases of the tadpole is added, does not have toxic or side effect or form residues in the body of the tadpole after being used, and prevents germs from medicament resistance and medicament tolerance; when a rana tadpole gender inducer is added into the feed according to a scientific proportion, malformed frogs are avoided while the proportion of female frogs is greatly improved; the bait waste caused by dissolution and sedimentation of the feed is reduced; accurate control over the feeding amount of the feed is realized; and the culturing cost is greatly reduced.

The invention relates to rana tiglina feed. The feed comprises components in parts by weight as follows: 6-10 parts of imported fish meal, 10-12.5 parts of chicken meal, 7.7-20.5 parts of soybean meal, 13-23 parts of fermented soybean meal, 20-21 parts of flour, 5-6 parts of wheat, 2-6 parts of corn protein powder, 5-7 parts of soy protein concentrate, 1-1.5 parts of squid paste, 1-2 parts of soybean phospholipid oil, 1-2 parts of soybean oil, 1.5-2 parts of fish oil, 2-3 parts of monocalcium phosphate, 0.2-0.3 part of choline chloride, 1-1.5 parts of vitamin premixed feed, 1-1.5 parts of mineral premixed feed, 0.1-0.3 part of lysine, 0.2-0.3 part of methionine and 0.01-0.02 part of an antioxidant. By matching of various nutrient substances, the use amount of the fish meal is substantiallyreduced and the nutrient requirement of rana tiglina is met, so that the rana tiglina feed is efficient and environmentally friendly.

A domestication method of a full-feed black-spotted frog species. In the first year, the eggs of the black-spotted frog are cultivated in a simulated wild environment and fed with live bait; in the second year, after the young frogs of the black-spotted frog complete metamorphosis and land , feed with live bait for one month, after that, gradually reduce the amount of live bait, add feed to the live bait for feeding, and gradually increase the proportion of feed until all feed is used for feeding; in the third year, in the black After the young frogs of spotted frogs complete their metamorphosis and come ashore, they are directly fed with feed, and the young frogs will have adverse reactions. After feeding with feed for one month, some young frogs are eliminated, and some young frogs are used to feeding with feed; in the fourth year, Black-spotted frogs are raised in the frog pond and fed with feed. The invention subverts the tradition, reduces the production cost, liberates a large amount of labor force, is suitable for large-scale industrial production, greatly shortens the growth cycle, greatly improves the output, the yield per mu reaches more than 3,000 jin, and the economic benefit is significantly increased.

The invention discloses a non-finger frog secretory peptide and gene and application in the drug in the biological medical domain, which codes a cyclic peptide with molecular weight at 1587.98 and isoelectric point at 9.7, wherein the primary structure of peptide is Phe Met Pro Ile Leu Ser Cys Ser Arg Phe Lys Arg Cys with disulfide bond in the seventh cysteine and thirteenth cysteine; the gene of secretory peptide is composed of 300 nucleic acids, which codes 138-177th nucleic acid; the peptide possesses obvious bacteria and fungi inhibiting action, which can be applied to make microbe infection disease drug.

The invention discloses a method for breeding rana catesbiana shaw. The method specifically comprises the following steps that step 1, a place close to a water resource is selected to carry out digging of a culture pond, the cross section of the culture pond is inverted trapezoidal, iron gauze is arranged on the edge of the culture pond, the height of the iron gauze is 1-1.5 meters; step 2, beforeyoung rana catesbiana shaw is put, firstly, glacial acetic acid is poured into the culture pond to disinfect the culture pond; step 3, after disinfection is completed, water containing the glacial acetic acid in the culture pond is discharged, then new water is introduced and then discharged after soaking is carried out for three days, new water is introduced again, and the step is repeated threetimes. The method for breeding rana catesbiana shaw has the advantages that feed made by mixing tenebrio molitor living bodies, maggot living bodies and animal giblets is fed, 60-70% of feed is evenly spread on a dry edge zone in the culture pond, thereby increasing the exercise amount of rana catesbiana shaw, so that the meat of rana catesbiana shaw becomes more compact, and the taste of rana catesbiana shaw is ensured.