The invention discloses a preparation method for lactic acid-producing bacillus subtilis microbial preparation. After undergoing primary fermentation and secondary fermentation, a seed culture solution is then transferred into a bran solid medium, and is fermented under appropriate conditions, and thereby the lactic acid-producing bacillus subtilis microbial preparation is prepared. The preparation method optimizes a fermentation medium, a thallus as the seed culture solution, more than 80 percent of which are formed into spores, is inoculated into the fermentation medium, and is subjected tothe primary fermentation and the secondary fermentation under the appropriate conditions, a secondary seed solution is then inoculated into the bran solid medium, and is controlled to ferment at highdensity under appropriate conditions, so that a large quantity of spores can be formed, and meanwhile, the affection of foreign bacteria is prevented. The preparation method effectively solves the problems of the bran fermentation process in the prior art, such as excessive foreign bacteria, uneven ventilation and heat dissipation, non-constant temperature and humidity and the like, and greatly increases the viable count of the bacilli subtilis; in a test, the viable count per gram of the lactic acid-producing bacillus subtilis microbial preparation prepared by the method is 20 to 25 billion,the spore rate is larger than 80 percent, and the foreign bacteria rate is 0.5 to 2 percent.