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Culture device and method for activity detection of soil hydrocarbon oxidation bacteria

A technology for cultivating equipment and hydrocarbon-oxidizing bacteria, which is applied in the direction of measuring equipment, biochemical cleaning equipment, biochemical equipment and methods, etc., can solve problems affecting the accuracy of hydrocarbon-oxidizing microorganisms, and achieve the solution of soil permeability and soil moisture Contradictions, the effect of ensuring reliability and improving efficiency

Active Publication Date: 2014-03-26
CHINA PETROLEUM & CHEM CORP +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This directly affects the accuracy of the determination of the activity of hydrocarbon oxidizing microorganisms

Method used

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  • Culture device and method for activity detection of soil hydrocarbon oxidation bacteria
  • Culture device and method for activity detection of soil hydrocarbon oxidation bacteria
  • Culture device and method for activity detection of soil hydrocarbon oxidation bacteria

Examples

Experimental program
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Embodiment 1

[0046] Such as figure 1 As shown, the culture device for detecting the activity of soil hydrocarbon oxidizing bacteria includes a detection bottle 1 and an injection needle that can be inserted into the detection bottle 1. The test bottle here is a serum bottle with a rubber stopper and a perforated cover. The materials used can be sterilized by high temperature.

[0047] Implementation steps:

[0048] (1) Test bottle Take a 50ml serum bottle and its corresponding rubber stopper and perforated outer cover. The bracket is made of high-temperature cooking plastic film. The production method is: cut a piece of plastic film with a width of 2cm and a length of 40cm and seal it with plastic. The machine is folded and welded every 4 cm along the length, and each welding section is separated by 1-2 cm. After welding, it is crimped along the length, clamped with nickel, and placed in the serum bottle. After it is loosened inside, adjust the Its flat. The carrying net can be made of degre...

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Abstract

The invention discloses a culture device and method for activity detection of soil hydrocarbon oxidation bacteria. The culture device comprises a detection bottle (1) and an injection needle capable of being inserted into the detection bottle (1), wherein the detection bottle (1) is a container or a device capable of forming an airtight space; a bracket (5) is arranged inside the detection bottle; water is formed below the bracket; a loading net (4) is paved and covered on the bracket; sealing of the detection bottle comprises sealing of a rubber plug (3) and a perforated cover (2). When the device is used, a gas is injected into or sucked from the detection bottle by the injection needle penetrating through the rubber plug. The culture device is improved on the basis of activity detection of the conventional soil hydrocarbon oxidation bacteria, the humidity inside the culture device can be increased, the gas permeability of a soil sample can be ensured, the methane utilization rate of methane-oxidizing bacteria and the hydrocarbon consumption rate of hydrocarbon oxidation bacteria are improved, and the reliability of activity detection is ensured. Thus, reliable technical means is supplied for application of a microbiological exploration technology.

Description

Technical field [0001] The present invention relates to a device for detecting the activity of soil hydrocarbon oxidizing bacteria in the field of soil microbial activity detection, and further, relates to a culture device and method for detecting the activity of soil hydrocarbon oxidizing bacteria used in the activity detection of hydrocarbon oxidizing bacteria . Background technique [0002] In the past two decades, with the improvement of microbial detection technology and the increase in international and domestic energy demand, microbial oil and gas exploration technology has shown vigorous vitality. The activity of gaseous hydrocarbon oxidizing bacteria in the soil is an important exploration index as much as its quantity. How to improve the accuracy of gaseous hydrocarbon oxidizing bacteria activity detection is one of the keys to improving microbial oil and gas exploration technology. [0003] The current general method for determining the activity of soil hydrocarbon oxid...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12Q1/04G01N30/02
CPCC12M23/08C12M23/38C12M23/48C12M29/00C12M41/36C12Q1/04
Inventor 任春黄欣汤玉平许科伟高国平杨帆赵克斌
Owner CHINA PETROLEUM & CHEM CORP
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