The invention discloses a method for detecting the activity of
skin flap
endonuclease 1 based on double
cascade signal amplification. Belongs to the technical field of
enzyme activity detection. According to the invention, the specific
dumbbell probe is designed; when the
skin flap
endonuclease 1 exists in the sample to be detected, the
skin flap
endonuclease 1 can be detected; a flap endonuclease 1 specifically recognizes and cuts off a 5'overhanging flap in the
dumbbell probe to generate a single-stranded
DNA with a 3 '-OH terminal and a
dumbbell-shaped
DNA with a notch, two products generated by
cutting respectively trigger rolling circle amplification and strand displacement amplification reactions, specific signals are amplified, the purpose of sensitive detection is achieved, the substrate
utilization rate is 100%, no probe is wasted, and the detection cost is reduced. The method is carried out under isothermal and homogeneous conditions, does not need high-precision thermal circulation and separation steps, does not need to prepare a functional nano material, does not relate to a biological
coupling procedure, effectively reduces the analysis cost and complexity, and provides a
technical support for the detection of the activity of the
skin flap endonuclease 1.