13 results about "Pheretima" patented technology

The invention relates to a pheretima bombycis farm, and belongs to the technical field of earthworm breeding. The problem that earthworm suffocation death is caused by cross contamination of pathogenic microorganisms and unsmooth drainage due to mixing of functional areas of a traditional farm is solved. The layout of the farm is specifically as follows: a raw material pretreatment and storage area provided with a fermentation tank is arranged in the upwind direction of a site entrance; a core breeding production area is arranged in the center of the field, the interior of the core breeding production area is divided into an earthworm breeding area, a breeding area and a fattening area according to breeding stages, and mechanical channels are arranged in the areas and are of a ridge type structure; arranging an annular drainage ditch along the boundary of the field, and communicating the furrow with the annular drainage ditch by gradient; a finished product treatment and storage area containing an airing field is arranged in the downwind direction of the field exit; an isolation belt is arranged on the periphery, a management and auxiliary area containing a tool room is arranged at an entrance, and a feces treatment area containing a sedimentation tank is arranged in the downwind direction; the hardened main road is connected with all the functional areas, and the branch is communicated with the breeding district. According to the scheme, pollution source isolation and efficient drainage are achieved, and the method is mainly used for improving the earthworm survival rate and breeding benefits.

The invention belongs to the field of breeding, and particularly relates to a feed for breeding lumbricus and a preparation method of the feed. The corn straw, the decomposed cow dung and the pseudo-ginseng dregs are used as core raw materials, and functional additives such as the oyster shell powder and the beer yeast paste are used as auxiliary materials, so that the carbon nitrogen ratio and trace elements (such as selenium) are optimized, and the nutritional and medicinal values are ensured. Wherein after the pseudo-ginseng decoction dregs are mixed with the honey water for fermentation, other components in the feed are supplemented, so that the growth of the pheretima can be effectively promoted, and the medicinal value of the pheretima can be improved. The main raw materials are pretreated and then mixed with the auxiliary materials to build a pile for fermentation, temperature-controlled pile turning is performed, pH is adjusted, efficient degradation and sterilization are achieved, and the feed capable of promoting growth of the lumbricus and improving the medicinal value of the lumbricus is obtained.

The invention discloses a traditional Chinese medicine formula (Shenhuang decoction) for treating postoperative wounds of high-order perianal abscess, belongs to the field of traditional Chinese medicines, and aims to solve the problems of wound infection, slow healing and obvious pain and edema after the high-order perianal abscess. The formula is based on 30 g of radix sophorae flavescentis, 20 g of cortex phellodendri, 15 g of radix angelicae, 6 g of radix arnebiae seu lithospermi, 15 g of frankincense, 15 g of radix angelicae sinensis, 10 g of rhizoma bletillae, 10 g of resina draconis and 3 g of borneol, and increase and decrease are carried out according to symptoms: when swelling is obvious, peach kernels and spina gleditsiae (leeches and earthworms are added, when secretion is more, astragalus membranaceus is reinforced, when bleeding occurs, pseudo-ginseng and hairyvein agrimony are added, honeysuckle stems are added when heat is excessive, and when the mouth is not closed for a long time, codonopsis pilosula, rhodiola rosea, gynostemma pentaphyllum and gallnut are added. The preparation adopts an ultrasonic atomization liquid form, is prepared by decocting with water and taking 400ml of juice (at 38 DEG C), and is atomized, fumigated and washed for 15 minutes once in the morning and once in the evening every day, and is matched with conventional dressing change. The traditional Chinese medicine composition can clear away heat and toxic materials, promote blood circulation to arrest pain, remove slough and promote granulation, reduce the level of TNF-alpha and IL-6, shorten the wound healing time and relieve pain and edema, and is suitable for wound recovery after high perianal abscess operation.

The invention relates to the technical field of earthworm breeding, and discloses an anti-escape device for earthworm breeding, which comprises a breeding tank, a transmission structure and a guide rail channel, the breeding groove is of a groove box structure with the top open, after rotating shafts are fixed to the two end walls of the breeding groove respectively, the rotating shafts on the two sides are movably matched in the same guide rail groove channel respectively, the rotating shafts are located at the upper position of the gravity center of the breeding groove, and a guide rail track formed by the guide rail groove channel is rectangular, and the two ends of the guide rail track form fillets respectively. The rotating shafts on the same side are further rotationally connected to the same transmission structure in a sleeving mode. Compared with the prior art, the breeding tank has the advantages that the inner wall of the breeding tank is smooth, earthworms are difficult to climb, the tank body can stably move in the guide rails, and earthworm escape caused by carrying shaking is avoided. According to the invention, synchronous and stable movement of a plurality of culture tanks is realized, and concentrated feeding, observation or harvesting is facilitated; the tank body can be quickly fixed at any station to adapt to different operation requirements; the overall movable base design facilitates overall carrying, cleaning or recombination layout of the equipment.

The invention belongs to the technical field of earthworm breeding, and discloses a breeding method for improving fresh weight of earthworms and enzyme yield of earthworms, which comprises the following steps: constructing a breeding foundation bed; domesticating the breeding earthworms: selecting the breeding earthworms, uniformly scattering the breeding earthworms on the breeding foundation bed, and continuously observing; segmented feeding is conducted, and in the young earthworm stage, a low-concentration diluted nutrient solution is sprayed to the breeding foundation bed; in the adult earthworm stage, the nutrient solution stock solution is used as one of feed and is matched with high-protein feed to feed earthworms; in the quality improvement period, only a high-concentration diluted nutrient solution is sprayed, and the high-concentration diluted nutrient solution is 50 times diluted liquid of the nutrient solution stock solution; and controlling the environment. According to the invention, the growth speed of lumbricus is obviously promoted, and the individual and unit area yield (fresh weight) of lumbricus is effectively improved; the synthesis and accumulation of medicinal active substances such as earthworm enzyme and earthworm kinase in the lumbricus are specifically stimulated, so that the medicinal quality of the lumbricus is improved; the palatability and the conversion rate of the feed are improved, ingestion of pheretima is promoted, feed residues are reduced, and the breeding environment is optimized.

The alpha-glucosidase inhibitory peptide is prepared by the following steps: with earthworm (eisenia fetida) as a raw material, carrying out autolysis enzymolysis pretreatment, then carrying out secondary enzymolysis by adopting flavourzyme, and carrying out ultrafiltration separation (selecting 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t); the polypeptide is obtained by carrying out LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) peptide sequence identification and screening in combination with PeptideRanker, AutodockVina and molecular docking software, and the amino acid sequences of the polypeptide are Arg-Gly-Phe (RGF) and Phe-Leu-Pro (FLP). The inhibitory activity of alpha-glucosidase is taken as an index, and the IC50 values of RGF and FLP are respectively 0.599 mg / mL and 1.344 mg / mL. The alpha-glucosidase inhibitory peptide provided by the invention can be used as an auxiliary hypoglycemic active component, and can be applied to functional products such as food, special medical food and medicines.

The invention provides an under-forest ecological breeding method for lumbricus, and the method specifically comprises the steps of base selection and preparation, seedling screening and breeding, feed preparation and base management in the breeding process, the ground surface fertilizer and soil humidity are regulated and controlled by controlling the shading, environment, soil quality, pH, environment humidity and temperature of a base, and the yield of the lumbricus is improved. According to the method, the breeding process temperature is controlled, and special feed is selected, so that the lumbricus can be continuously and stably bred, the influence of environmental conditions is avoided, the lumbricus medicinal material resource with stable quality can be continuously provided, and the problem that the lumbricus resource is threatened due to the fact that a large number of wild lumbricus is captured can be solved to a certain extent.

The present application belongs to the technical field of biological medicine, and particularly relates to an earthworm anti-platelet oligopeptide and application thereof. The present application is an anti-platelet oligopeptide discovered by combining a Pheretima aspergillum (E. Perrier) transcriptome database with active-oriented separation. The oligopeptide can not only inhibit the activation and aggregation of platelets, but also effectively inhibit the adhesion and expansion of platelets, and can effectively inhibit various functions of platelets at an in-vitro level. In summary, the oligopeptide of the present application can significantly inhibit platelets, and is suitable for anti-thrombus drug research and development.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a pharmaceutical composition containing a high-molecular complex for external use on skin and a preparation method of the pharmaceutical composition, and the pharmaceutical composition comprises the following components in percentage by weight: 0.1-0.3% of sodium humate; 0.8 to 1.4% of a bacteriostatic composition; 0.8 to 1.4% of a soothing composition; 0.12 to 0.14 percent of chlorhexidine acetate; 6-20% of a humectant; 1-3% of an emulsifier; 0.1%-0.5% of a functional auxiliary material; the balance of pure water; the antibacterial composition comprises a glabrous sarcandra herb extract, a radix sophorae flavescentis extract and a fructus cnidii extract; the soothing composition comprises an earthworm extract and a fructus kochiae extract. The sodium humate is compounded with other components, so that the inhibition effect of the composition on microorganisms such as candida albicans, staphylococcus aureus and escherichia coli can be improved. Meanwhile, the sodium humate can effectively repair and relieve skin mucosa, so that the skin is quickly recovered to a healthy state.

The utility model relates to an indoor lumbricus raising device based on temperature regulation and control of circulating water, which belongs to the technical field of biological medicine raw material cultivation and comprises a first circulating water outer container and a second circulating water outer container which are both in a rectangular block shape and are internally and fixedly provided with a raising box. A layer of soil is laid at the bottom of the feeding box to simulate the natural living environment of pheretima. The first circulating water outer container and the second circulating water outer container are connected through a communicating pipe and are both connected with the circulating water temperature regulation and control mechanism, so that the indoor artificial temperature regulation and control feeding effect of pheretima is achieved. The feeding quality and yield can be improved, and the limitation of earthworm feeding is broken through.

This invention discloses a method for promoting the growth and nutrient metabolism of radish tubers using earthworms in conjunction with nano-lanthanum oxide, belonging to the field of nano-agricultural regulation technology. The method of this invention, by simultaneously adding specific amounts of La2O3NMs and a specific density of *Pheretima spp.* in the soil, significantly promotes the tuber enlargement of cherry radishes, increases the net photosynthetic rate and sucrose content of cherry radish leaves, upregulates the expression of IAA biosynthetic genes in the radish epidermis, and ultimately promotes tuber epidermal growth. Furthermore, it increases the content of functional nutrients (D-tryptophan and sinapic acid) in the cherry radish tuber pulp and antioxidants (kaempferol, trifolin, calciferol 3-O-arabinoside, phlorizin) in the tuber epidermis, and increases the abundance of IAA-producing bacteria (flavobacterium) in the rhizosphere, but decreases the abundance of nitrogen cycle-related denitrifying microorganisms (Massilia), thereby increasing the rhizosphere nitrate nitrogen content and promoting nutrient absorption, growth, and physiological metabolism of cherry radish tubers.

The invention belongs to the technical field of biological medicine, and particularly relates to earthworm anticoagulant oligopeptide and application thereof. The invention relates to an anticoagulant oligopeptide, which is found by combining the activity-directed separation of pheretima asper (E. Perriver) with the screening of an AI (artificial intelligence) prediction model. The amino acid sequence of the oligopeptide is LMWFSDRNK; the oligopeptide regulates thrombin in an allosteric manner, enhances the combination of thrombin and antithrombin, and can effectively inhibit blood coagulation and thrombus formation at in-vivo and in-vitro levels; the oligopeptide has a remarkable anticoagulant effect and is suitable for research and development of antithrombotic drugs.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition and a preparation and application thereof. The traditional Chinese medicine composition is composed of a first component, a second component and a third component according to the mass ratio of 1: (1-2): (0.2-0.8), wherein the first component contains oldenlandia diffusa, sculellaria barbata and gynostemma pentaphylla, is a monarch drug and directly attacks damp toxin and blood stasis; the second component contains radix astragali, rhizoma atractylodis macrocephalae, semen coicis, polyporus umbellatus, poria cocos and taxus chinensis, is a ministerial drug and an adjuvant drug, and is used for invigorating spleen to eliminate dampness and inducing diuresis to And the third component comprises curcuma zedoary, pseudo-ginseng and lumbricus which are used as conductant drugs and can promote blood circulation to remove meridian obstruction and harmonize the drugs. Experiments and clinical verifications show that the traditional Chinese medicine composition provided by the invention can significantly improve proteinuria, regulate lipid metabolism, protect kidney structure and delay fibrosis, is suitable for patients with different conditions, is scientific in compatibility and convenient to prepare, and can be used for preparing medicines for treating membranous nephropathy with syndrome of damp toxin and blood stasis.