128 results about "Pheretima" patented technology

The invention discloses a method for identifying a DNA bar code molecule of an earthworm and a COI sequence of the DNA bar code molecule of the earthworm. The method comprises the steps of amplifying the gene of a sample COI through polymerase chain reaction (PCR), verifying the PCR product by agarose gel, then sending to a biological sequencing company for sequencing, according to the sequencing result, sequence splicing through manual correcting, comparing with a public sequence, then confirming the tissue to be tested as the source of pheretima aspergillum or pheretima vulgaris if the homology of the spliced sequence and the gene sequence SEQ ID NO.1-NO.4 is over 99%. By adopting the reliable DNA molecular identification technology, the method can rapidly and accurately identify the earthworm varieties specified by the 'pharmacopoeia of China' and non-medicinal material earthworm varieties without conducting morphological identification on the earthworm varieties, can further distinguish the pheretima aspergillum varieties from pheretima vulgaris varieties, improves the accuracy and reliability, and guarantees the safety of the earthworm as the medicinal material.

The invention discloses a loop-mediated isothermal amplification detection primer of Pheretima cantonensis and a method for identifying Pheretima cantonensis based on an LAMP technology. The inventiondesigns and screens a set of specific detection primers, a detection kit containing the detection primers and an identification method for determining whether a sample to be tested is Cantonese earthworm through loop-mediated isothermal amplification by using the detection kit for determining whether the sample is Cantonese earthworm or not. The invention is simple in operation, the result detection only needs naked eye observation, does not need expensive detection instrument, and the sensitivity is 1000 times higher than that of conventional PCR. The invention has lower cost and can be applied to authenticity identification of earthworm commodities.

The invention relates to the field of traditional Chinese medicines, in particular to a cough relieving medicine containing honey-fried ephedra herb and baical skullcap roots and a preparation methodand a detection method thereof. The cough relieving medicine containing honeyed ephedra herb and baical skullcap root is prepared from the following medicinal raw materials in parts by weight: 110-125g of honeyed ephedra herb, 110-125g of mulberry bark, 225-240g of honeysuckle flower, 110-125g of baical skullcap root, 205-220g of thesium chinensis herb, 205-220g of gypsum, 110-125g of thunberbg fritillary bulb, 110-125g of armeniacae semen amarum, 110-125g of purple perilla fruit, 110-125g of common coltsfoot flower, 110-125g of semen lepidii, 205-220g of pheretima, 75-90g of ginger processedpinellia tuber, 110-125g of stir-fried ginkgo seed and 75-90g of honey-fried licorice root. The invention provides a method for simultaneously measuring the contents of active components of the coughrelieving medicine containing honey-fried ephedra herb and baical skullcap root by adopting a high performance liquid chromatography. The invention also provides new application of the cough relievingmedicine containing honey-fried ephedra herb and baical skullcap root in treatment of chronic obstructive pulmonary diseases.

The invention provides a herb incense stick, which is prepared from the following raw materials in parts by weight including: 15-30 parts of radix angelicae sinensis, 15-30 parts of radix paeoniae alba, 15-30 parts of fructus lycii, 15-25 parts of radix achyranthis bidentatae, 15-25 parts of jujube, 15-25 parts of common floweringquince fruit, 15-25 parts of caulis spatholobi, 15-25 parts of fructus corni, 10-20 parts of fructus toosendan, 10-20 parts of tall GasTCMLIBodia tuber, 10-20 parts of ramulus uncariae cum uncis, 10-30 parts of concha haliotidis, 10-20 parts of concha margaritifera, 1-3 parts of borneolum syntheticum, 1-5 parts of pheretima, 1-5 parts of apple, 5-15 parts of snow pear, 5-15 parts of lemon, 3-12 parts of rhizoma zingiberis recens, 1-5 parts of garlic, 1-5 parts ofgreen onion, and 1-5 parts of white radish. The invention also provides a preparation process of the herb incense stick. The preparation process of the herb incense stick in the invention is simple, and the cost is low. The prepared herb incense stick is convenient to use, non-toxic and harmless, and may effectively purify blood and cauterize cells alive, thus achieving the effect of making humanblood clean and cells energetic.

The invention discloses a method for breeding pheretima seedlings, which includes the selection of suitable culturing zone, the selection and arrangement of the culturing field, the feed and delivery, the earthworm sowing, the field management, the prevention and control of diseases, insects, pests, weeds and damages, and the harvesting of earthworms. The method for breeding pheretima seedlings solves the breeding problem of pheretima seedlings, provides the high-quality seedlings of earthworms for pheretima, provides the guarantee for the pheretima aquaculture industry, solves the shortage of medicinal materials, and also provides the quality guarantee for the Chinese medicinal materials of pheretima. The method for breeding pheretima seedlings can also be used for breeding seedlings ofother southern medicinal earthworms.

The invention discloses a traditional Chinese medicine composition capable of treating gastritis, gastric ulcer and duodenal ulcer and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 7 to 15 parts of caulis dendrobii, 4 to 9 parts of fructus citri sarcodactylis, 5 to 6 parts of rhizoma cyperi, 10 to 15 parts of endothelium corneum gigeriae galli, 4 to 6 parts of Chinese angelica, 4 to 10 parts of herba taraxaci, 3 to 5 parts of bighead atractylodes rhizome, 4 to 6 parts of radix aucklandiae, 3 to 7 parts of fructus aurantii immaturus, 5 to 12 parts of cuttlefish bone, 4 to 6 parts of liquorice root, 5 to 8 parts of rhizoma bletillae, 4 to 9 parts of fructus jujubae and 8 to 12 parts of pheretima. The traditional Chinese medicine composition has a remarkable curative effect on the gastritis, the gastric ulcer and the duodenal ulcer and the healing effect is obvious and is better than various treatment methods and medicines at present; the traditional Chinese medicine composition is high in safety and has no toxic side effect; the medicine can be used for adjusting chief cells, parietal cells and mucous cells and the cells are maintained in a normal level range and kept balanced; the medicine can be used for treating both principal and secondary aspect of disease; all the drugs are scientifically matched and the mutual coordination effect is sufficiently expressed; the traditional Chinese medicine composition has the effects of converging and engendering flesh, nourishing yin and clearing away heat, enhancing the appetite, treating, nourishing and protecting stomach and has the characteristics of low cost, rapid effect and lasting curative effect.

The invention discloses a Chinese medicine composition for treating hypertension and improving hyperlipidemia and a preparation method of the Chinese medicine composition. The Chinese medicine composition comprises the following components in parts by weight: 10 to 20 parts of semen cassia, 8 to 16 parts of fleece-flower root, 10 to 20 pars of pheretima, 10 to 20 parts of raw haws, 10 to 20 partsof wild tea, 10 to 20 parts of kudzuvine root powder, 8 to 16 parts of rhizoma alismatis, 8 to 16 parts of white mulberry root bark and 10 to 20 parts of young uncaria. The preparation method comprises the following steps of: a, weighing the semen cassia, the fleece-flower root, the pheretima, the raw haws and the kudzuvine root powder according to the parts in weight; after pulverizing, screening with a sieve of 300 meshes to form medicinal powder; b, weighing the wild tea, the rhizoma alismatis, the white mulberry root bark and the young uncaria according to the parts in weight; adding water; decocting to form thick juice; and c, mixing and uniformly stirring the medicinal powder and the medicinal juice; drying; and preparing into a desired medicinal dosage form. The Chinese medicine composition provided by the invention has a smoother hypotensive effect in comparison with western medicines, does not have any toxic or side effect, and has efficacy of enhancing organism immunity and senescence resistance.

The invention discloses a formula composition capable of reducing blood pressure and a preparation method thereof which relates to the technical field of pharmacy. The formula composition capable of reducing the blood pressure is prepared from the following components in parts by weight: 20 parts of male flower of eucommia ulmoides, 35 parts of pheretima protein powder, 20 parts of chrysanthemum morifolium flower, 20 parts of radix puerariae lobatae, 20 parts of folium ilicis latifoliae, 20 parts of radix arctii, 20 parts of semen cassiae, 20 parts of flos sophorae immaturus, 15 parts of fructus lycii, 20 parts of fructus crataegi, 20 parts of folium ginkgo, 20 parts of semen coicis, 30 parts of tartary buckwheat, 10 parts of celery seeds, 10 parts of corn stigma and an appropriate amount of nattokinase extract. The formula composition disclosed by the invention can be prepared into tablets, pills, granules or capsules according to a conventional method after being prepared according to the components, has the advantages of good effect, convenience in taking and convenience in carrying, is capable of avoiding a situation of curing the symptoms but not the disease and cannot damage other positions of a body.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a pheretima detection kit and a preparation method thereof. The kit comprises a genome extraction system, a PCR system and a detection system. The method comprises the steps of sample pretreatment, genome extraction, PCR, result identification and the like. Earthworm sample pretreatment involves dry samples and wet samples. According to an earthworm DNA detection method includes the steps that earthworm genome specificity oligonucleotide primers are designed, the primers are artificially synthesized, a reaction program is determined, and results are judged. Whether earthworms exist in medicinal earthworm raw materials or not is accurately judged. The identification method is simple, convenient and quick to use and reliable in identification result.

The invention discloses a traditional Chinese medicinal composition for treating the angina pectoris of coronary heart disease and a preparation method thereof. The traditional Chinese medicinal composition is prepared from the following bulk drugs in parts by weight: 15 to 30 parts of pollen typhae, 15 to 35 parts of herba lycopi, 10 to 30 parts of fructus crataegi, 20 to 30 parts of rhizoma corydalis, 15 to 45 parts of caulis spatholobi, 10 to 30 parts of semen persicae, 10 to 30 parts of flos carthami, 15 to 25 parts of hirudo, 15 to 30 parts of pheretima, 5 to 20 parts of sangusis draconis, 20 to 45 parts of radix salviae miltiorrhizae, 20 to 35 parts of fructus trichosanthis and 10 to 35 parts of radix cyathulae. The traditional Chinese medicinal composition provided by the inventionhas the functions of activating blood circulation, relieving pain, eliminating phlegm, removing blood stasis, clearing and activating channels and collaterals, invigorating the kidney and nourishing the heart, and is applicable to treating patients suffered from a coronary heart disease with the symptoms of blood stasis, phlegm obstruction and heart and kidney deficiencies at the same time and theangina pectoris.

The invention discloses a method for transporting pheretima asperfillm larvae. According to the method, transport carrier feed is loaded in a transport case, the pheretima asperfillm larvae are placedin the transport carrier feed, the relative humidity in the transport case is controlled in 45%-75%, the temperature is 15 DEG C-30 DEG C, and then the transport case is transported to the destination; the transport carrier feed is a mixture of peat soil and cow dung or a mixture of farmland soil and cow dung; venting holes are formed in the wall of the transport case. The method carries out an experiment aiming at the problems such as a transport carrier, feed, density, temperature, humidity control and the like in the transportation process, and the transportation is convenient by screeningcarriers and reducing weight; the feeding amount of earthworm larvae under different conditions is tested, and the problem of feed use amount in the transportation is solved; the carrier feed ratio is screened for optimizing to solve the transportation density problem; research and development are conducted on transportation equipment to solve the temperature and humidity control problem. The method can solve the transportation problem of the pheretima asperfillm larvae, and the survival rate during the transportation is 97% or above.

The invention discloses a traditional Chinese medicine composition treating facial neuritis, a preparation method and application thereof. The traditional Chinese medicine composition A comprises the following Chinese medicinal raw materials in parts by weight: 5-15 parts of pheretima, 12-18 parts of rhizoma et radix notopterygii, 12-18 parts of radix saposhnikoviae, 12-18 parts of radix angelicae sinensis, 12-18 parts of radix angelicae sinensis, 12-18 parts of herba lycopodii, 6-12 parts of rhizoma corydalis, 6-12 parts of ramulus cinnamomi, and 6-12 parts of herba ephedrae. The preparation method comprises the steps: uniformly mixing the Chinese medicinal raw materials, and preparing according to a routine method. The traditional Chinese medicine composition is simple in preparation, small in toxic and side effect, excellent in curative effect and obvious in effect, is capable of effectively shortening disease course, is usable in a facial neuritis acute phase, and is convenient for nursing and usage, and easy to store.

The invention discloses a recipe of a traditional Chinese medicine for treating meningitis. The recipe comprises the following components according to a proportional ratio by weight: 3g to 10g of lamium amplexicaule, 5g to 50g of radix rehmanniae praeparata, 3g to 30g of rhizoma dioscoreae, 3g to 30g of cortex moutan radicis, 3g to 30g of rhizoma alismatis, 3g to 30g of poria cocos, 2g to 15g of fructus schisandrae chinensis, 3g to 30g of fructus corni, 3g to 50g of radix glehniae, 3g to 60g of radix astragali seu hedysari, 35g to 60g of fructus psoraleae, 5g to 30g of folium mori, 5g to 15g of flos chrysanthemi indici, 2g to 20g of semen persicae, 3g to 30g of radix paeoniae rubra, 3g to 20g of pheretima, 3g to 30g of rhizoma chuanxiong, 3g to 50g of radix ophiopogonis and 5g to 40g of radix salviae miltiorrhizae. The traditional Chinese medicine has the advantages that the adverse reaction and any toxic or side effect are avoided in application; the traditional Chinese medicine is effective and safe.

The invention relates to a liver softening beverage prepared through using Tribulus Terrestris, Pheretima aspegillum and Chinese Taxillus Twig. The beverage is characterized in that raw materials for preparing the effective components of the beverage comprise the Tribulus Terrestris, the Pheretima aspegillum and the Chinese Taxillus Twig. The beverage provided by the invention has the advantages of convenient carrying, low price, use convenience, and effective reaching of liver tonifying and protecting effects.

The invention relates to the field of medicine preparation, in particular to a traditional Chinese medicine composition for treating gout. The traditional Chinese medicine composition is characterized by comprising, by weight, 8-11% of pine nodular branch, 7-9% of Herba Sarcandrae, 7-9% of Herba Abri, 7-9% of Herba Commelinae, 2-5% of Herba Siegesbeckiae, 2-5% of Herba Lycopodii, 6-9% of Herba Glechomae, 2-4% of Radix Zanthor Nu lum Nitidum, 3-5% of Rhizoma Cibotii, 3-5% of Caulis Sargentodoxae, 3-5% of Rhizome Dioscorea Nipponica, 2-4% of Caulis Trachelospermi, 4-7% of Carapax et Plastrum Testudinis, 2-4% of Rhizome Panacis Majoris, 2-3% of Radix Gentianae Macrophyllae, 6-9% of Rhizoma Smilacis Glabrae, 2-4% of Pheretima, 1-2% of Valeriana jatamansi, 2-3% of Bulbus Allii Macrostemonis, 2-3% of Bombyx Batryticatus, 2-4% of Lignum Sappan and 2-4% of Caulis Piperis Kadsurae. A preparation method of the traditional Chinese medicine composition includes soaking the raw materials with water, decocting and then filtering residues to obtain filter liquor. The traditional Chinese medicine composition has the advantages of low cost, high efficacy in treating gout and no toxic and side effect and avoids diseases recurrence.

The invention discloses medicine for treating burns and scalds and a preparation method thereof .The medicine is prepared from, by weight, 40-60 parts of fresh pheretima, 5-15 parts of white sugar, 40-60 parts of fresh aloes and 1-3 parts of borneolum syntheticum .The medicine is prepared from pure natural Chinese herbal medicine, is free of toxic or side effect, quick in anti-inflammatory and pain-relieving effects, high in muscle growth function and short in wound healing time .The medicine is utilized to treat 10 cases of patients suffering from small-area shallow II-degree and III-degree scalds without purulent infection, as a result, the symptoms are controlled within 24 hours, and decrustation and recovery are achieved in 4-7 days.

The invention discloses a compound traditional Chinese medicinal preparation for treating ischemic cerebrovascular diseases. The compound traditional Chinese medicinal preparation is of granules prepared from the following raw materials in parts by weight: 30 parts of Bubalus bubalis horn, 15 parts of Salvia miltiorrhiza, 10 parts of radix paeoniae rubra, 10 parts of Ligusticum wallichii, 10 parts of radix Curcumae, 10 parts of wine-treated rhubarb, 10 parts of pheretima, 10 parts of rhizoma acori graminei, 30 parts of raw oyster shell, and 10 parts of concretio silicea bambusae. The compound traditional Chinese medicinal preparation disclosed by the invention is simple in preparation process and low in production cost, has the effects of clearing away heat and toxic materials, promoting blood circulation and dispelling blood stasis, has a good therapeutic action on ischemic cerebrovascular diseases, and has the effects of inhibiting platelet aggregation and inflammatory cell infiltration, resisting free radical damage, relieving hydrocephalus and clinical symptoms and improving microcirculation and achieving the protective action to ischemic brain tissues.

The invention discloses a traditional Chinese medicine preparation for treating asthma. The traditional Chinese medicine preparation is made of, by weight, 5-20 parts of poria, 6-30 parts of exocarpium citri rubrum, 5-35 parts of pheretima, 8-24 parts of ramulus cinnamomi, 5-25 parts of radix glycyrrhizae preparata, 6-15 parts of semen armeniacae amarum, 5-18 parts of fructus schisandrae chinensis, 3-14 parts of processed herba ephedrae, 6-16 parts of millet hulls and 2-10 parts of radix platycodonis. The traditional Chinese medicine preparation has the advantages that the traditional Chinese medicine preparation comprises reasonable components, is convenient to take and free of toxic and side effects, and as proved by clinical tests, the effective rate reaches 96% after the traditional Chinese medicine preparation is administered for three treatment courses.

The invention relates to an oral medicine for treating asthma, and a preparation method thereof, and aims to solve problems that traditional Chinese medicine takes effect slowly while the curative effect of western medicine can not meet patients' satisfaction. The oral medicine is prepared by raw drug materials according to the following weight ratio: 150-300 of pheretima, 2.5-5 of promethazine hydrochloride, 20-50 of radix asteris, 50-100 of white ammonium chloride, 20-50 of radix stemonae, 56-112 of extractum glycyrrhizae, 1-2 of camphor and 1-2 of star anise oil. The preparation method comprises the following steps of (1) smashing a specified amount of pheretima, promethazine hydrochloride, radix asteris, white ammonium chloride, radix stemonae, and camphor into fine powder respectively; and (2) mixing the smashed raw drug materials with a specified amount of extractum glycyrrhizae and star anise oil thoroughly, and making into tablets, pulvis and capsules or other oral preparations. The oral medicine has the functions of relieving asthma, tranquilization, moistening lung to relive cough and resisting allergy, can be used for treating bronchitis, emphysema and asthma, takes effect quickly, and has high cure rate.

The invention provides a patch for treating bone and joint diseases. The patch comprises the following formula: 10 to 30 parts of nidus vespae, 15 to 30 parts of prepared frankincense, 15 to 30 parts of prepared myrrh, 20 to 30 parts of cassia twig, 10 to 30 parts of safflower, 20 to 30 parts of radix aconiti preparata, 10 to 30 parts of radix clematidis, 25 to 30 parts of peach kernel, 25 to 30 parts of rhizoma homalomenae, 15 to 30 parts of Chinese angelica, 25 to 30 parts of radix et rhizoma salviae miltiorrhizae, 25 to 40 parts of caulis piperis futokadsurae, 25 to 30 parts of rhizoma atractylodis, 15 to 30 parts of liquorice root, 25 to 40 parts of scorpion, 10 to 40 parts of fried pheretima, 20 to 30 parts of red paeony root and 15 to 30 parts of radix angelicae pubescentis. The patch for treating the bone and joint diseases, provided by the invention, can be used for carrying out adjuvant therapy on bone fracture painful diseases including osteoarthritis and the like; the patch has a scientific and reasonable prescription and a medicine is absorbed in a transdermal manner; in a treatment process, a medicine effect can directly reach a wound and articular pain and swelling are rapidly alleviated; the patch has a rapid effect and no toxic and side effects and has a good long-term curative effect.

The invention discloses a traditional Chinese medicine preparation for curing eczema and a preparation method thereof. The traditional Chinese medicine preparation for curing eczema is prepared from the following materials in parts by weight: 70-130 parts of schizonepeta, 70-130 parts of radix saposhnikoviae, 170-230 parts of radix paeoniae rubra, 170-230 parts of gypsum fibrosum, 90-150 parts of fructus mume, 90-150 parts of periostracum cicada, 120-180 parts of zaocys dhumnade, 120-180 parts of radix sophorae flavescentis, 120-180 parts of pheretima, 200-400 parts of fructus kochiae, 200-400 parts of cortex dictamni, 200-400 parts of cortex lycii radices, 40-80 parts of liquorice, 120-180 parts of common bombax flower, 120-180 parts of folium mori, 120-180 parts of Hangzhou chrysanthemum, 120-180 parts of microcos paniculata, 0.001-0.002 part of acesulfame, 0.001-0.002 part of sodium benzoate, and 0.001-0.0016 part of essence. The traditional Chinese medicine preparation has the effects of dispelling wind and clearing heat and dispelling dampness and relieving itching, is obvious in curative effect, is high in effective rate, and is safe and non-toxic.