6 results about "Pheretima" patented technology

The invention relates to the technical field of earthworm breeding, and discloses an anti-escape device for earthworm breeding, which comprises a breeding tank, a transmission structure and a guide rail channel, the breeding groove is of a groove box structure with the top open, after rotating shafts are fixed to the two end walls of the breeding groove respectively, the rotating shafts on the two sides are movably matched in the same guide rail groove channel respectively, the rotating shafts are located at the upper position of the gravity center of the breeding groove, and a guide rail track formed by the guide rail groove channel is rectangular, and the two ends of the guide rail track form fillets respectively. The rotating shafts on the same side are further rotationally connected to the same transmission structure in a sleeving mode. Compared with the prior art, the breeding tank has the advantages that the inner wall of the breeding tank is smooth, earthworms are difficult to climb, the tank body can stably move in the guide rails, and earthworm escape caused by carrying shaking is avoided. According to the invention, synchronous and stable movement of a plurality of culture tanks is realized, and concentrated feeding, observation or harvesting is facilitated; the tank body can be quickly fixed at any station to adapt to different operation requirements; the overall movable base design facilitates overall carrying, cleaning or recombination layout of the equipment.

The invention belongs to the technical field of earthworm breeding, and discloses a breeding method for improving fresh weight of earthworms and enzyme yield of earthworms, which comprises the following steps: constructing a breeding foundation bed; domesticating the breeding earthworms: selecting the breeding earthworms, uniformly scattering the breeding earthworms on the breeding foundation bed, and continuously observing; segmented feeding is conducted, and in the young earthworm stage, a low-concentration diluted nutrient solution is sprayed to the breeding foundation bed; in the adult earthworm stage, the nutrient solution stock solution is used as one of feed and is matched with high-protein feed to feed earthworms; in the quality improvement period, only a high-concentration diluted nutrient solution is sprayed, and the high-concentration diluted nutrient solution is 50 times diluted liquid of the nutrient solution stock solution; and controlling the environment. According to the invention, the growth speed of lumbricus is obviously promoted, and the individual and unit area yield (fresh weight) of lumbricus is effectively improved; the synthesis and accumulation of medicinal active substances such as earthworm enzyme and earthworm kinase in the lumbricus are specifically stimulated, so that the medicinal quality of the lumbricus is improved; the palatability and the conversion rate of the feed are improved, ingestion of pheretima is promoted, feed residues are reduced, and the breeding environment is optimized.

The alpha-glucosidase inhibitory peptide is prepared by the following steps: with earthworm (eisenia fetida) as a raw material, carrying out autolysis enzymolysis pretreatment, then carrying out secondary enzymolysis by adopting flavourzyme, and carrying out ultrafiltration separation (selecting 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t, 1t); the polypeptide is obtained by carrying out LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) peptide sequence identification and screening in combination with PeptideRanker, AutodockVina and molecular docking software, and the amino acid sequences of the polypeptide are Arg-Gly-Phe (RGF) and Phe-Leu-Pro (FLP). The inhibitory activity of alpha-glucosidase is taken as an index, and the IC50 values of RGF and FLP are respectively 0.599 mg / mL and 1.344 mg / mL. The alpha-glucosidase inhibitory peptide provided by the invention can be used as an auxiliary hypoglycemic active component, and can be applied to functional products such as food, special medical food and medicines.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a pharmaceutical composition containing a high-molecular complex for external use on skin and a preparation method of the pharmaceutical composition, and the pharmaceutical composition comprises the following components in percentage by weight: 0.1-0.3% of sodium humate; 0.8 to 1.4% of a bacteriostatic composition; 0.8 to 1.4% of a soothing composition; 0.12 to 0.14 percent of chlorhexidine acetate; 6-20% of a humectant; 1-3% of an emulsifier; 0.1%-0.5% of a functional auxiliary material; the balance of pure water; the antibacterial composition comprises a glabrous sarcandra herb extract, a radix sophorae flavescentis extract and a fructus cnidii extract; the soothing composition comprises an earthworm extract and a fructus kochiae extract. The sodium humate is compounded with other components, so that the inhibition effect of the composition on microorganisms such as candida albicans, staphylococcus aureus and escherichia coli can be improved. Meanwhile, the sodium humate can effectively repair and relieve skin mucosa, so that the skin is quickly recovered to a healthy state.

The utility model relates to an indoor lumbricus raising device based on temperature regulation and control of circulating water, which belongs to the technical field of biological medicine raw material cultivation and comprises a first circulating water outer container and a second circulating water outer container which are both in a rectangular block shape and are internally and fixedly provided with a raising box. A layer of soil is laid at the bottom of the feeding box to simulate the natural living environment of pheretima. The first circulating water outer container and the second circulating water outer container are connected through a communicating pipe and are both connected with the circulating water temperature regulation and control mechanism, so that the indoor artificial temperature regulation and control feeding effect of pheretima is achieved. The feeding quality and yield can be improved, and the limitation of earthworm feeding is broken through.

The invention belongs to the technical field of biological medicine, and particularly relates to earthworm anticoagulant oligopeptide and application thereof. The invention relates to an anticoagulant oligopeptide, which is found by combining the activity-directed separation of pheretima asper (E. Perriver) with the screening of an AI (artificial intelligence) prediction model. The amino acid sequence of the oligopeptide is LMWFSDRNK; the oligopeptide regulates thrombin in an allosteric manner, enhances the combination of thrombin and antithrombin, and can effectively inhibit blood coagulation and thrombus formation at in-vivo and in-vitro levels; the oligopeptide has a remarkable anticoagulant effect and is suitable for research and development of antithrombotic drugs.