Feed for breeding lumbricus and preparation method thereof

Abstract

The invention belongs to the field of breeding, and particularly relates to a feed for breeding lumbricus and a preparation method of the feed. The corn straw, the decomposed cow dung and the pseudo-ginseng dregs are used as core raw materials, and functional additives such as the oyster shell powder and the beer yeast paste are used as auxiliary materials, so that the carbon nitrogen ratio and trace elements (such as selenium) are optimized, and the nutritional and medicinal values are ensured. Wherein after the pseudo-ginseng decoction dregs are mixed with the honey water for fermentation, other components in the feed are supplemented, so that the growth of the pheretima can be effectively promoted, and the medicinal value of the pheretima can be improved. The main raw materials are pretreated and then mixed with the auxiliary materials to build a pile for fermentation, temperature-controlled pile turning is performed, pH is adjusted, efficient degradation and sterilization are achieved, and the feed capable of promoting growth of the lumbricus and improving the medicinal value of the lumbricus is obtained.

Description

Technical Field

[0001] The invention belongs to the field of breeding, and in particular relates to a feed for breeding Lumbricus radiatus and a preparation method thereof. Background Art

[0002] As a core species in the traditional Chinese medicine market, Pheretima aspergillum holds an irreplaceable position within the Lingnan authentic medicinal system. Its pharmacological value stems primarily from its highly active lumbrokinase, clinically proven to increase cerebral thrombus dissolution by over 50%. However, its physiological characteristics make it significantly more challenging to cultivate artificially than other earthworm species. Its unique locomotion pattern (deep burrowing and surface feeding) creates a unique soil pore structure that maintains intestinal microbial diversity. Its intestinal enzyme system absorbs nutrients less efficiently than that of ordinary earthworms, necessitating meticulous feed composition, such as a strict carbon-to-nitrogen ratio. Imbalanced carbon-to-nitrogen ratios can lead to skin corrosion and reduce survival rates.

[0003] Due to the indiscriminate hunting and killing of wild Dilong, the ecological environment has been severely damaged, and artificial breeding has gradually become more important. However, compared with most farmed species, Dilong is difficult to breed, has a low survival rate, and is more feral. Research in this area is still in its early stages of exploration. There is no mature feed or technology for artificially cultivating Dilong seedlings. Not only is the survival rate low, but the medicinal properties of artificially cultivated Dilong are also slightly less than those of wild Dilong. Therefore, there is an urgent need for a new feed for cultivating Dilong that can improve the survival rate and medicinal properties of artificially cultivated Dilong.

[0004] CN 114009599 A discloses an artificial breeding feed for promoting the rapid growth of P. guangxiong. The feed uses livestock and poultry waste, straw, fruit and vegetable waste, and multidimensional amino acid glucose as components, and strictly controls the carbon-nitrogen ratio. It can promote the rapid growth of P. guangxiong seedlings and improve the soil microbial environment. However, there is no exploration into improving the survival rate and medicinal properties of artificially bred P. guangxiong. Summary of the Invention

[0005] In view of the deficiencies in the prior art, the present invention provides a feed for breeding Pteris lucidum and a preparation method thereof.

[0006] A feed for breeding earthworms, comprising the following components by mass percentage:

[0007] 30-40wt% pulverized corn stover;

[0008] 30-50wt% decomposed cow dung;

[0009] 5-15wt% fermented Panax notoginseng residue;

[0010] 6-10wt% oyster shell powder;

[0011] 3-8wt% beer yeast mud;

[0012] 0.001-0.005wt% sodium selenite;

[0013] 0.5-2wt% stachyose.

[0014] Preferably, the feed for breeding P. luxuriantii consists of the following components by mass percentage:

[0015] 33-38wt% pulverized corn stover;

[0016] 35-45wt% decomposed cow dung;

[0017] 8-12wt% fermented Panax notoginseng residue;

[0018] 7-9wt% oyster shell powder;

[0019] 4-6wt% beer yeast slurry;

[0020] 0.001-0.003wt% sodium selenite;

[0021] 0.5-1.5wt% stachyose.

[0022] Preferably, the feed for breeding P. guangxiong is composed of the following components by mass percentage:

[0023] 35.998wt% pulverized corn stover;

[0024] 40wt% decomposed cow dung;

[0025] 10wt% fermented Panax notoginseng residue;

[0026] 8wt% oyster shell powder;

[0027] 5 wt% beer yeast slurry;

[0028] 0.002wt% sodium selenite;

[0029] 1wt% stachyose.

[0030] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0031] (1) Raw material pretreatment: corn straw is processed into 1-2 cm fragments by a hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung is mixed with zeolite powder (wherein the amount of zeolite powder added is 4-6wt% of the weight of the cow dung), piled for 36-72 hours, and turned 1-3 times to obtain decomposed cow dung; Panax notoginseng residue is sprayed with molasses water at a concentration of 1.5-3wt% and a spraying amount of 6-10wt% of the weight of the Panax notoginseng residue, and sealed and fermented at 39-41°C for 60-84 hours to obtain fermented Panax notoginseng residue;

[0032] (2) According to the percentage by mass, 30-40wt% of crushed corn stalks, 30-50wt% of decomposed cow dung, 5-15wt% of fermented Panax notoginseng residue, 3-8wt% of brewer's yeast paste, and 0.5-2wt% of stachyose are put into a blender and mixed evenly to obtain raw materials. Water is added to 65wt% of the raw material mass, and EM bacterial liquid is inoculated at an inoculum amount of 4-6mL / kg. The stirring is continued for 10-20min, and then the mixture is poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2m and a height of 1m, and covered with linen to keep it moist. The pile is turned over on the 3rd, 5th, and 7th days, and maintained at 55-60℃ for 72-96h. When the temperature is higher than 65℃, it is immediately spread out to air. On the 4th day, oyster shell powder is sprinkled in, and the mass of oyster shell powder accounts for 6-10wt% of the total mass of the raw materials. The pH of the raw materials is adjusted to 6.8-7.2. The fermentation is completed after 7 days to obtain the fermentation raw materials.

[0033] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.001-0.005 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0034] The sodium selenite solution contains 0.1-0.2 g / L sodium selenite.

[0035] Beneficial effects of the present invention:

[0036] This invention aims to produce a feed for cultivating Lumbricus radiatus through balanced raw material selection, scientific proportioning, and phased process control. This feed achieves a high Lumbricus radiatus survival rate and enhances its medicinal properties. The formula primarily uses agricultural waste, maintains cost control, and ensures stable feed quality through three stages: pretreatment, primary fermentation, and post-processing.

[0037] The present invention uses corn straw, decomposed cow dung, and Panax notoginseng residue as core raw materials, supplemented with functional additives such as oyster shell powder and brewer's yeast paste, to optimize the carbon-nitrogen ratio and trace elements (such as selenium) to ensure nutritional and medicinal value. First, crushing corn straw is conducive to increasing the fermentation rate; second, the cow dung is mixed with zeolite powder for pre-piling to reduce the amount of ammonia released by the manure; finally, the present invention found that after the Panax notoginseng residue is mixed with honey water for fermentation, supplemented with other components in the feed, it can effectively promote the growth of Lu Lu and enhance its medicinal value. The present invention speculates that this is because the triterpenoid saponins remaining in the Panax notoginseng residue are hydrophobic substances, which are almost impossible to be absorbed by Lu Lu when fed directly. After mixed with honey water for fermentation, the saponin glycosides can be effectively hydrolyzed, the polarity of the aglycone is reduced, and it penetrates the epidermis of Lu Lu and improves the absorption efficiency. This hydrolyzed saponin glycoside can effectively promote the growth of Lu Lu and enhance its medicinal value. After pretreatment, the main raw materials are mixed with auxiliary materials to build a pile for fermentation, control the temperature and turn the pile, and adjust the pH to achieve efficient degradation and sterilization, resulting in a feed that can promote the growth of Lulong and enhance its medicinal value. DETAILED DESCRIPTION

[0038] The following examples further illustrate the specific implementation of the present invention. If there are any processes not specifically described in detail, they can be implemented or understood by those skilled in the art with reference to the prior art. Reagents or instruments used without manufacturer indication are considered to be commercially available conventional products.

[0039] EM bacterial solution, product number: Bj-sw, Shandong Beijia Biotechnology Co., Ltd.

[0040] Stachyose, CAS: 10094-58-3.

[0041] Example 1

[0042] A feed for breeding earthworms, comprising the following components by mass percentage:

[0043] 35.998wt% pulverized corn stover;

[0044] 40wt% decomposed cow dung;

[0045] 10wt% fermented Panax notoginseng residue;

[0046] 8wt% oyster shell powder;

[0047] 5 wt% beer yeast slurry;

[0048] 0.002wt% sodium selenite;

[0049] 1wt% stachyose.

[0050] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0051] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the weight of the cow dung), piled for 48 h, and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sprayed with 2 wt% molasses water at a spraying amount of 8 wt% of the weight of the Panax notoginseng residue, and fermented at 40 ° C for 72 h to obtain fermented Panax notoginseng residue;

[0052] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of fermented Panax notoginseng residue, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65 wt% of the raw material mass, and EM bacterial solution was inoculated at an inoculum rate of 5 mL / kg. The mixture was stirred for 15 min, and then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and maintained at 58 ° C for 72 h. When the temperature was higher than 65 ° C, it was immediately spread out to air. On the 4th day, oyster shell powder was sprinkled in, and the mass of oyster shell powder accounted for 8 wt% of the total mass of the raw material. The pH of the raw material was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0053] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0054] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0055] Example 2

[0056] A feed for breeding earthworms, comprising the following components by mass percentage:

[0057] 35.998wt% pulverized corn stover;

[0058] 40wt% decomposed cow dung;

[0059] 10wt% fermented Panax notoginseng residue;

[0060] 8wt% oyster shell powder;

[0061] 5 wt% beer yeast slurry;

[0062] 0.002wt% sodium selenite;

[0063] 1wt% stachyose.

[0064] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0065] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was piled for 48 h and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sprayed with 2 wt% molasses water at a spraying amount of 8 wt% of the mass of Panax notoginseng residue and fermented at 40 °C for 72 h to obtain fermented Panax notoginseng residue;

[0066] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of fermented Panax notoginseng residue, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65 wt% of the raw material mass, and EM bacterial solution was inoculated at an inoculum rate of 5 mL / kg. The mixture was stirred for 15 min, and then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and maintained at 58 ° C for 72 h. When the temperature was higher than 65 ° C, it was immediately spread out to air. On the 4th day, oyster shell powder was sprinkled in, and the mass of oyster shell powder accounted for 8 wt% of the total mass of the raw material. The pH of the raw material was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0067] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0068] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0069] Example 3

[0070] A feed for breeding earthworms, comprising the following components by mass percentage:

[0071] 35.998wt% pulverized corn stover;

[0072] 40wt% decomposed cow dung;

[0073] 10wt% fermented Panax notoginseng residue;

[0074] 8wt% oyster shell powder;

[0075] 5 wt% beer yeast slurry;

[0076] 0.002wt% sodium selenite;

[0077] 1wt% stachyose.

[0078] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0079] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the cow dung mass), piled for 48 h, and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sealed and fermented at 40°C for 72 h to obtain fermented Panax notoginseng residue;

[0080] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of fermented Panax notoginseng residue, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65 wt% of the raw material mass, and EM bacterial solution was inoculated at an inoculum rate of 5 mL / kg. The mixture was stirred for 15 min, and then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and maintained at 58 ° C for 72 h. When the temperature was higher than 65 ° C, it was immediately spread out to air. On the 4th day, oyster shell powder was sprinkled in, and the mass of oyster shell powder accounted for 8 wt% of the total mass of the raw material. The pH of the raw material was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0081] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0082] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0083] Example 4

[0084] A feed for breeding earthworms, comprising the following components by mass percentage:

[0085] 35.998wt% pulverized corn stover;

[0086] 40wt% decomposed cow dung;

[0087] 10wt% Panax notoginseng residue;

[0088] 8wt% oyster shell powder;

[0089] 5 wt% beer yeast slurry;

[0090] 0.002wt% sodium selenite;

[0091] 1wt% stachyose.

[0092] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0093] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the mass of cow dung), piled for 48 h, and turned over twice to obtain decomposed cow dung;

[0094] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of Panax notoginseng residue, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65 wt% of the raw material mass, and EM bacterial solution was inoculated at an inoculum rate of 5 mL / kg. The mixture was stirred for 15 min, and then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and maintained at 58 ° C for 72 h. When the temperature was higher than 65 ° C, it was immediately spread out to dry. On the 4th day, oyster shell powder was sprinkled in, and the mass of oyster shell powder accounted for 8 wt% of the total mass of the raw material. The pH of the raw material was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0095] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0096] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0097] Example 5

[0098] A feed for breeding earthworms, comprising the following components by mass percentage:

[0099] 35.998wt% pulverized corn stover;

[0100] 40wt% decomposed cow dung;

[0101] 10wt% fermented Panax notoginseng residue;

[0102] 8wt% oyster shell powder;

[0103] 5 wt% beer yeast slurry;

[0104] 0.002wt% sodium selenite;

[0105] 1wt% stachyose.

[0106] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0107] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the weight of the cow dung), piled for 48 h, and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sprayed with 2 wt% molasses water at a spraying amount of 8 wt% of the weight of the Panax notoginseng residue, and fermented at 40 ° C for 72 h to obtain fermented Panax notoginseng residue;

[0108] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of fermented Panax notoginseng residue, 8 wt% of oyster shell powder, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain the raw material. Water was added to 65 wt% of the raw material mass, and EM bacterial liquid was inoculated at an inoculum rate of 5 mL / kg. The mixture was stirred for 15 min, then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and kept at 58 °C for 72 h. When the temperature was higher than 65 °C, it was immediately spread out to air. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0109] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0110] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0111] Comparative Example 1

[0112] The difference from Example 1 is that: fermented Panax notoginseng residue is not added, and the mass ratios of other components remain unchanged;

[0113] The mass ratio of crushed corn straw, decomposed cow dung, oyster shell powder, brewer's yeast paste, sodium selenite and stachyose is 35.998:40:8:5:0.002:1.

[0114] Comparative Example 2

[0115] A feed for breeding earthworms, comprising the following components by mass percentage:

[0116] 35.998wt% pulverized corn stover;

[0117] 40wt% decomposed cow dung;

[0118] 10wt% fermented Panax notoginseng residue;

[0119] 8wt% oyster shell powder;

[0120] 5 wt% beer yeast slurry;

[0121] 0.002wt% sodium selenite;

[0122] 1wt% stachyose.

[0123] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0124] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the weight of the cow dung), piled for 48 h, and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sprayed with 2 wt% molasses water at a spraying amount of 8 wt% of the weight of the Panax notoginseng residue, and fermented at 40 ° C for 72 h to obtain fermented Panax notoginseng residue;

[0125] (2) According to the percentage by mass, 35.998 wt% of crushed corn stalks, 40 wt% of decomposed cow dung, 10 wt% of fermented Panax notoginseng residue, 5 wt% of brewer's yeast paste, and 1 wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65 wt% of the raw material mass and the mixture was stirred for 15 min. The mixture was then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2 m and a height of 1 m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days and kept at 58 °C for 72 h. When the temperature was higher than 65 °C, it was immediately spread out to air. On the 4th day, oyster shell powder was sprinkled in. The mass of oyster shell powder accounted for 8 wt% of the total mass of the raw materials. The pH of the raw materials was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw materials.

[0126] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0127] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0128] Comparative Example 3

[0129] A feed for breeding earthworms, comprising the following components by mass percentage:

[0130] 35.998wt% pulverized corn stover;

[0131] 40wt% decomposed cow dung;

[0132] 10wt% fermented Panax notoginseng residue;

[0133] 8wt% oyster shell powder;

[0134] 5 wt% beer yeast slurry;

[0135] 0.002wt% sodium selenite;

[0136] 1wt% stachyose.

[0137] The present invention also provides a method for preparing feed for breeding P. guangxiong, comprising the following steps:

[0138] (1) Raw material pretreatment: corn straw was processed into 1-2 cm fragments by hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung was mixed with zeolite powder (the amount of zeolite powder added was 5 wt% of the weight of the cow dung), piled for 48 h, and turned over twice to obtain decomposed cow dung; Panax notoginseng residue was sprayed with 2 wt% molasses water at a spraying amount of 8 wt% of the weight of the Panax notoginseng residue, and fermented at 40 ° C for 72 h to obtain fermented Panax notoginseng residue;

[0139] (2) According to the mass percentage, 35.998wt% of crushed corn straw, 40wt% of decomposed cow dung, 10wt% of fermented Panax notoginseng residue, 5wt% of brewer's yeast paste, and 1wt% of stachyose were put into a blender and mixed evenly to obtain raw materials. Water was added to 65wt% of the raw material mass, and EM bacterial liquid was inoculated at an inoculum rate of 5mL / kg. The mixture was stirred for 15min, then poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2m and a height of 1m. The pile was covered with linen to keep it moist. The pile was turned over on the 3rd, 5th, and 7th days, and maintained at 58℃ for 72h. When the temperature was higher than 65℃, it was immediately spread out to air. On the 7th day, oyster shell powder was sprinkled in, and the mass of oyster shell powder accounted for 8wt% of the total mass of the raw material. The pH of the raw material was adjusted to 7.0. The fermentation was completed after 7 days to obtain the fermentation raw material.

[0140] (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.002 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

[0141] The sodium selenite solution contains 0.1 g / L sodium selenite.

[0142] Test Example 1

[0143] Survival rate test of Dilong: Select 80m 2 Breeding beds, Examples 1-5 and Comparative Examples 1-3 were divided into 8 groups, each with 10 m 2 The breeding beds were prepared using the appropriate feed for Lumbricus spp., with a 5 cm thick layer of material, covered with wet straw to maintain moisture. 100 Lumbricus spp. seedlings were placed per square meter, and their 30-day survival rate was measured. The results are shown in Table 1.

[0144] Table 1: Survival rate test results of Dilong dasyphylla

[0145] 30-day survival rate / % Example 1 92.7 Example 2 81.3 Example 3 85.1 Example 4 78.6 Example 5 80.9 Comparative Example 1 73.8 Comparative Example 2 68.4 Comparative Example 3 75.2

[0146] Test Example 2

[0147] Detection of the medicinal value of Guangdilong: Select 80m 2 Breeding beds, Examples 1-5 and Comparative Examples 1-3 were divided into 8 groups, each with 10 m 2 The breeding bed was prepared with the feed corresponding to the breeding of Lumbricus spp., and the material was laid according to the area of ​​the breeding bed, with a thickness of 5 cm, and covered with wet straw to keep it moist. 100 Lumbricus spp. seedlings were cultured per square meter. The lumbrokinase activity was tested after 90 days of culture. Two seedlings were randomly selected per square meter for testing, and the average value was taken. The results are shown in Table 2.

[0148] Table 1: Test results of the medicinal value of Dilong guang

[0149] Lumbrokinase activity (U / g) Example 1 237.6 Example 2 206.5 Example 3 192.8 Example 4 178.3 Example 5 188.7 Comparative Example 1 158.4 Comparative Example 2 143.2 Comparative Example 3 166.9

[0150] As can be seen from Tables 1 and 2, the feed for breeding P. chinensis prepared in the embodiments of the present invention significantly improved the survival rate and medicinal value of P. chinensis compared to the comparative examples, with Example 1 having the most significant effect. The present invention believes that this is because Example 1 of the present invention uses corn stalks, decomposed cow dung, and Panax notoginseng residue as core raw materials, supplemented with functional additives such as oyster shell powder and brewer's yeast paste, optimizes the carbon-nitrogen ratio and trace elements (such as selenium), and ensures nutritional and medicinal value. First, corn stalk crushing is conducive to improving fermentation rate; Second, cow dung is mixed with zeolite powder in advance to fertilize the pile and reduce the amount of manure ammonia released; Finally, the present invention finds that after the mixed fermentation of Sanqi medicinal residues with honey water, supplemented by other components in the feed, it can effectively promote the growth of Lu Lu and improve its medicinal value. The present invention speculates that this is because the triterpenoid saponins remaining in the Sanqi medicinal residues are hydrophobic substances, and directly feeding Lu Lu can hardly absorb them. After mixed fermentation with honey water, the saponin glycosides can be effectively hydrolyzed to reduce the polarity of the aglycone, penetrate the Lu Lu epidermis, and improve absorption efficiency. The saponin glycosides after this hydrolysis can effectively promote the growth of Lu Lu and improve its medicinal value. The main raw materials are mixed with auxiliary materials after pretreatment to build a pile for fermentation, temperature control and turning, and pH is adjusted to achieve efficient degradation and sterilization, and a feed that can promote the growth of Lu Lu and improve its medicinal value is obtained. In addition, Example 1 also regulates pH by oyster powder, regulates the fermentation bacteria process, and optimizes the fermentation effect.

[0151] In Example 2, cow dung is not added with zeolite powder, resulting in an imbalance in carbon-nitrogen ratio, and ammonia nitrogen exceeds the standard and corrodes the epidermal respiratory organs of P. chinensis. At the same time, the lack of zeolite causes heavy metal dissolution, inhibits enzyme catalytic activity, and feed performance declines. In Example 3, honey water is not added, and the notoginseng slag is not activated, resulting in a decline in saponin bioavailability, affecting P. chinensis absorption. In Example 4, the notoginseng slag is not fermented, and directly mixed with other raw materials for fermentation. The survival rate of the P. chinensis cultivated and its medicinal value further decline also prove this point. Comparative Example 1 does not add notoginseng slag, and its effect further declines compared with Example 4. The comparison of Example 1, Example 3, Example 4 and Comparative Example 1 can clearly see that after the notoginseng slag is mixed with honey water for fermentation, it is supplemented with other components in the feed, and the growth of P. chinensis can be effectively promoted, and its medicinal value is improved, and the technological development of P. chinensis rapid and efficient artificial cultivation and cultivation has been promoted.

[0152] In summary, the present invention uses three core ingredients of crushed corn stalks, decomposed cow dung, and fermented Panax notoginseng residue, supplemented by oyster shell powder, yeast paste, and selenium, and innovatively ferments and activates the Panax notoginseng residue with honey water, thereby significantly improving the permeability of hydrophobic components in the epidermis of Dilong radix, promoting the growth of Dilong radix, and improving its medicinal value, and promoting the development of technology for rapid and efficient artificial cultivation and breeding of Dilong radix.

Claims

1. A feed for breeding Lumbricus spp., characterized in that: By mass percentage, it is composed of the following components: 30-40wt% pulverized corn stover; 30-50wt% decomposed cow dung; 5-15wt% fermented Panax notoginseng residue; 6-10wt% oyster shell powder; 3-8wt% beer yeast mud; 0.001-0.005wt% sodium selenite; 0.5-2wt% stachyose.

2. The feed for breeding Pterostilbene as claimed in claim 1, wherein: The method for treating decomposed cow dung comprises the following steps: mixing cow dung with zeolite powder, piling for 36-72 hours, and turning the pile 1-3 times to obtain decomposed cow dung, wherein the amount of zeolite powder added is 4-6 wt% of the mass of the cow dung.

3. The feed for breeding Pterostilbene as claimed in claim 1, wherein: The method for treating fermented Panax notoginseng residue comprises the following steps: spraying the Panax notoginseng residue with molasses water having a concentration of 1.5-3wt% and a spraying amount of 6-10wt% of the mass of the Panax notoginseng residue, and sealing and fermenting at 39-41°C for 60-84h to obtain the fermented Panax notoginseng residue.

4. A method for preparing feed for breeding Pterostilbene as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Raw material pretreatment: corn straw is processed into 1-2 cm fragments by a hammer mill and passed through a 10-mesh sieve to obtain crushed corn straw; cow dung is mixed with zeolite powder, piled for 36-72 hours, and turned 1-3 times to obtain decomposed cow dung, wherein the amount of zeolite powder added is 4-6wt% of the mass of the cow dung; Panax notoginseng residue is sprayed with molasses water at a concentration of 1.5-3wt% and a spraying amount of 6-10wt% of the mass of the Panax notoginseng residue, and sealed and fermented at 39-41℃ for 60-84 hours to obtain fermented Panax notoginseng residue; (2) According to the mass percentage, 30-40wt% of crushed corn straw, 30-50wt% of decomposed cow dung, 5-15wt% of fermented Panax notoginseng residue, 3-8wt% of brewer's yeast paste, and 0.5-2wt% of stachyose are put into a blender and mixed evenly to obtain raw materials. Water is added and EM bacterial liquid is inoculated. The stirring is continued for 10-20 minutes, and then the mixture is poured out of the blender and piled into a trapezoidal pile with a bottom width of 1.2m and a height of 1m. The pile is covered with linen to keep it moist. The pile is turned over on the 3rd, 5th, and 7th days, and maintained at 55-60℃ for 72-96h. When the temperature is higher than 65℃, it is immediately spread out to air. On the 4th day, oyster shell powder is sprinkled in to adjust the pH of the raw materials to 6.8-7.

2. The fermentation is completed after 7 days to obtain the fermentation raw materials. (3) Spraying a sodium selenite solution into the fermentation raw material obtained in step (2) at a rate of 0.001-0.005 wt% of the mass of the sodium selenite to the total mass of the raw material, stirring evenly, and obtaining the feed for breeding Lumbricus spp.

5. The method for preparing feed for breeding Pteris lucidum according to claim 4, wherein: The inoculation volume of the EM bacterial solution in step (2) is 4-6 mL / kg.

6. The method for preparing feed for breeding Pteris lucidum according to claim 4, wherein: The mass of the oyster shell powder in step (2) accounts for 6-10wt% of the total mass of the raw materials.

7. The method for preparing feed for breeding Pteris lucidum according to claim 4, wherein: The amount of water added in step (2) is 65wt% of the raw material mass.

8. The method for preparing feed for breeding Pteris lucidum according to claim 4, characterized in that: The sodium selenite solution in step (3) contains 0.1-0.2 g / L sodium selenite.