The invention discloses a method for detecting
platelet factor-4 without depending on
antibody, which belongs to the technical field of
biology. The method comprises the following steps: split-charging magnetic beads labeled with -NH2 or -COOH functional groups into a PCR tube, and then placing the PCR tube on a magnetic processor and removing liquid in the tube; taking the PCR tube out, then adding
buffer solution into the PCR tube, and mixing the mixture evenly; placing the PCR tube on the magnetic processor and removing the
buffer solution in the tube; taking the PCR tube out, adding serum or
blood plasma sample into the PCR tube, and mixing the mixture evenly and then standing the mixture; placing the PCR tube on the magnetic processor and removing the liquid in the tube; taking the PCR tube out, adding the
buffer solution into the PCR tube, and mixing the mixture evenly; placing the PCR tube on the magnetic processor and removing the buffer solution in the PCR tube; taking the PCR tube out, adding eluent into the PCR tube, and mixing the mixture evenly and then standing the mixture; placing the PCR tube on the magnetic processor and removing the liquid in the tube; and mixing the sucked liquid and saturated
sinapic acid solution evenly, sucking the
mixed solution to a target of matrix-assisted
laser resolution
ionization mass spectrometry, and putting the target into a
mass spectrometer to detect the target after the solution on the target is crystallized. The method has the advantages of low price and reliable detection result.