252 results about "Cerebral ischaemia" patented technology

The invention discloses an intelligent decision-making method for a cerebral ischemia drug administration time window, and relates to the technical field of intelligent decision-making. The method comprises the following steps: S1, acquiring parameters, and setting a cerebral ischemia drug configuration scheme; s2, extracting a medicine characteristic parameter set and a medicine taking influence parameter set; s3, calculating a drug combination risk parameter set; s4, performing hierarchical classification processing on the drug combination risk parameter set, and performing drug correlation analysis; s5, performing weighted fusion on a correlation analysis result in combination with a drug influence attenuation rule, generating a drug administration time window evaluation parameter, and adding a label; and S6, according to the parameters, the labels and the configuration scheme, generating a cerebral ischemia drug administration time window intelligent decision scheme, and performing storage and backup. According to the method, by combining directional extraction of the drug characteristic parameter set and the drug taking influence parameter set with Pearson correlation algorithm screening, decision errors caused by data deviation are avoided.

The invention belongs to the field of medicines, and discloses application of a stress-induced stem cell extract in treatment of cerebral apoplexy. The inventor finds that stem cells are cultured under proper stress conditions through screening, and the separated stem cell extract (also known as Alritoin) can increase the axon length and dendritic length of normal primary neurons and is beneficial to growth and conduction of nerve fibers; in a pdMCAO model, the volume of cerebral infarction in the acute stage of ischemia of a mouse can be remarkably reduced, the neurological function score can be improved, and a relatively good improvement trend is achieved in the aspect of cognition; in the acute stage of cerebral ischemia reperfusion, the pharmaceutical composition has remarkable effects on reducing the cerebral infarction volume of a mouse, reducing relative edema and improving the neurological function, and is relatively long in medication time window; in a cerebral ischemia reperfusion model, the trend of improving local cerebral blood flow is shown. The allitoin is expected to be developed into a promising drug for cerebral apoplexy, especially a drug for treating ischemic cerebral apoplexy.

The invention relates to a traditional Chinese medicine composition for treating ischemic stroke and a method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition for treating ischemic stroke. The traditional Chinese medicine composition is prepared from the following raw materials: ginseng, pseudo-ginseng, radix polygoni multiflori preparata, ligusticum wallichii, safflower carthamus, salvia miltiorrhiza, hawthorn, loranthus parasiticus, longspur epimedium, radix puerariae, cornu bubali, ginseng leaves, rhizoma acori graminei and borneol. According to the preparation method disclosed by the invention, the ligusticum wallichii and compound bacteria consisting of lactobacillus plantarum, bacillus subtilis and coriolus hirsutus are fermented, so that more laccase and active substances are generated in a fermented product, and then the fermented product is subjected to compound enzyme treatment, so that the processed ligusticum wallichii is better in medicine effect, and the curative effect of the ligusticum wallichii is improved. Therefore, the treatment effect of the traditional Chinese medicine composition for treating ischemic stroke is enhanced, neurological dysfunction caused by cerebral ischemia can be remarkably relieved, and the traditional Chinese medicine composition has a good protection effect on ischemic stroke nerve injury.

The invention discloses a treatment formula acting on dizziness and a preparation method thereof, the treatment formula comprises the following raw materials: 50-70 parts of Poria cocos, 60-80 parts of radix bupleuri, 40-50 parts of cassia twig, 10-20 parts of bighead atractylodes rhizome, 20-30 parts of gastrodia elata, 12-21 parts of dried orange peel, 12-25 parts of white peony root, 5-12 parts of licorice, 4-12 parts of dragon bone, 3-8 parts of red sage root, and 3-8 parts of pinellia ternate, and the invention relates to the technical field of medicine preparation. According to the treatment formula acting on dizziness and the preparation method thereof, the dizziness symptom can be relieved, for example, the dizziness symptom is relieved, the balance capacity is improved, the dizziness attack frequency and duration are reduced, cerebral ischemia and cerebral blood supply insufficiency are improved, neural function recovery is facilitated, the memory is enhanced, meanwhile, through cooperation of the medicinal materials, the physique of a patient can be improved, and the curative effect is good. The headache and dizziness symptoms are fundamentally treated, and the effect is good.

The invention relates to an astragaloside-loaded astragalus membranaceus exosome which is prepared by the following steps: crushing astragalus membranaceus, and filtering to obtain juice; performing multi-step differential centrifugation to obtain supernate; carrying out ultrafiltration and centrifugation, and collecting the precipitate; adding to the upper layer of a sucrose stepped gradient solution, centrifuging, and collecting part of strips; after dilution, centrifuging, discarding the supernatant, precipitating, and removing cane sugar to obtain the astragalus membranaceus exosome; the radix astragali exosome loaded with the astragaloside is obtained by mixing the radix astragali exosome with the astragaloside and carrying out ultrasonic treatment or co-incubation, and can be used for preparing medicines for specifically inhibiting cGAS / STING pathway excessive activation in cerebral ischemia reperfusion injury and downstream NLRP3 inflammasome mediated pyroptosis and preparing medicines for protecting and repairing mitochondria in the cerebral ischemia reperfusion injury. The homologous astragalus membranaceus exosome is used as a natural carrier, the blood-brain barrier is effectively overcome, and the enrichment concentration and bioavailability of astragaloside in a cerebral ischemia area are remarkably improved.

The invention relates to a bionic nano CO generator as well as preparation and application thereof, belongs to the technical field of medicines, and solves the problem that a CO delivery system which can synergistically promote neuroprotection and neurogenesis and has brain targeting and controlled release capabilities is urgently required to be developed at present. According to the technical scheme, the bionic nano CO generator comprises a drug-loaded particle inner core and a covering film, the drug-loaded particle inner core comprises hollow mesoporous CeO2 nano particles and MnCO loaded on the hollow mesoporous CeO2 nano particles, and the covering film is a macrophage membrane and wraps the drug-loaded particle inner core. The compound is used for apoplexy treatment administration by integrating neuroprotection and enhancing innovative dual channels of endogenous nerve growth, and is expected to significantly reduce brain injury after cerebral apoplexy and restore neurological functions so as to relieve the worry about high death rate and long-term disability after cerebral ischemia-reperfusion injury.

The invention belongs to the field of biological medicines, and provides a construction method of a photochemically induced mouse cerebral ischemia model, which comprises the following steps: S1, inducing anesthesia of a mouse, and ensuring that the mouse is continuously in an anesthesia state; s2, in the lateral position of the mouse, unhairing and disinfecting the face on one side, and cutting from the face of the mouse to expose the proximal end position of the M3 segment of the middle cerebral artery of the mouse or the cross point of reverse extension lines of branch blood vessels of the middle cerebral artery; s3, injecting a photosensitizer into the mouse, and after the photosensitizer circulates uniformly in the body of the mouse, irradiating the proximal end of the M3 segment of the middle cerebral artery or the cross point of the reverse extension line of the branch blood vessel of the middle cerebral artery after laser focusing; s4, after laser irradiation is finished, the skin is sutured, anesthesia is stopped, and the wound of the mouse is coated with an analgesic. The model obtained by the construction method of the photochemically induced mouse cerebral ischemia model provided by the invention has a larger infarct volume, and the infarct invades the subcortical brain region, so that the pathological characteristics of ischemic brain tissues are more obvious.

The invention discloses an image processing and simulation analysis method for a carotid artery image, which comprises the following steps: firstly, acquiring a CTA image, observing and selecting, then importing the CTA image, adjusting the image contrast, generating a three-dimensional blood vessel model, extracting a carotid artery focus, and carrying out overall and local surface optimization processing on the model. The method comprises the steps that firstly, a model is built, then local surface cutting naming and grid division are conducted on the model, finally parameters are set, CFD hemodynamic simulation is conducted, a simulation result is subjected to visualization processing, and preoperative and postoperative data are compared and analyzed. According to the method, multi-modal imaging data of brain CT perfusion imaging, brain CT angiography and the like of a patient suffering from carotid artery stenosis can be collected to be used for reconstructing a three-dimensional model of the carotid artery stenosis, the carotid artery hemodynamic characteristics of the patient and the individual characteristics of the patient are analyzed in an auxiliary mode based on computational fluid mechanics, operation mode selection is optimized, and the three-dimensional model of the carotid artery stenosis is reconstructed. Cerebral ischemia reperfusion injury is avoided, clinical prognosis of a patient is improved, and life quality of the patient is improved.

The invention discloses a traditional Chinese medicine composition for improving cerebral ischemia and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparation. The preparation method comprises the following steps: mixing, stirring and dispersing a polyurethane pressure-sensitive adhesive, a copper sulfide-polyacrylate glue solution, mixed traditional Chinese medicine powder, ethyl acetate and a penetration enhancer, and standing to obtain a drug-loaded composite pressure-sensitive adhesive; and coating a polyethylene backing layer with the drug-loaded composite pressure-sensitive adhesive, and then drying, evaporating and curing to obtain the traditional Chinese medicine composition. Ginseng is used as a monarch drug, astragalus membranaceus is used as a ministerial drug, fructus aurantii, caulis spatholobi, poria cocos, gastrodia elata and coptis chinensis are used as adjuvant drugs, and liquorice is used as a conductant drug, so that the whole traditional Chinese medicine composition can enhance qi and blood, improve microcirculation, protect neurons and reduce ischemia consequences, and can regulate the drug properties of all the drugs to play a synergistic effect. And then preparing the traditional Chinese medicine composition patch with photo-thermal penetration promotion to realize the treatment of cerebral ischemia.

The invention provides an application of combination of indobufen and butylphthalide in preparation of a drug for preventing and / or treating cerebrovascular diseases, indobufen and butylphthalide are combined for use, have a synergistic effect, remarkably improve the treatment effect, can be used for preparing the drug for preventing, secondarily preventing or treating the cerebrovascular diseases, and has a wide application prospect. The medicine is especially suitable for treating cerebral apoplexy and transient ischemic attack, and can be used for prophylactically improving cerebral infarction caused by cerebral arterial thrombosis.

The invention relates to the technical field of biological medicine, in particular to 7-dehydrocholesterol liposome and application thereof. According to the invention, a biological membrane modified liposome is constructed, and the biological membrane modified liposome comprises an ROS responsive liposome, a neutrophile granulocyte membrane and a renal tubular epithelial cell membrane; the biofilm modified liposome has an active oxygen species (ROS) responsive release function and a dual targeting capability, has the advantages of an integrated drug effect carrier, ROS triggered release, dual bionic targeting, strong target cell uptake and multi-mechanism ferroptosis resistance, and can be used for preparing the biofilm modified liposome. The compound can be used for treating ferroptosis related diseases such as acute kidney injury, myocardial ischemia-reperfusion injury and cerebral ischemia-reperfusion injury, and has wide clinical popularization value.

The utility model belongs to the field of experimental devices, and relates to a 360-degree rotating device capable of continuously transmitting bilateral electroencephalogram and myoelectricity of a freely moving rat for a long time. The device is mainly composed of a small conductive slip ring, a slip ring stator end wire protection cover, a slip ring rotor end wire fixing clamping box, a double-row pin header and an acrylic fixing shelf. A wire at a stator end of the small conductive slip ring is welded with the two double-row contact pins and is a bilateral electroencephalogram and electromyographic signal transmitting end; a wire at the rotor end of the slip ring is welded with the double-row pin header and is a bilateral electroencephalogram and electromyographic signal input end; and the main body of the slip ring is fixed on the acrylic shelf through a flange. The novel experimental device is small and light, stable in signal transmission, low in cost and capable of respectively recording electroencephalograms of brains on the left side and the right side of the rat for a long time and providing powerful help for basic experimental researches such as evaluation of brain function states and prognosis of injuries such as cerebral ischemia, evaluation of treatment effects and screening of new intervention measures.

This invention discloses a class of aminosalicylic acid derivative compounds, their pharmaceutical compositions, and uses. The aminosalicylic acid derivatives of this invention can significantly improve ischemic symptoms in rats with a cerebral ischemia-reperfusion model, and these compounds have broad application prospects in the preparation of drugs for the prevention and treatment of stroke.

The present invention recognizes that there is a need for the prophylaxis or treatment of Stroke Related Disorders, which includes but is not limited to stroke, ischemic stroke, hemorrhagic stroke, transient ischemic attack (TIA), and related cardiovascular diseases, or combinations thereof, or combinations thereof. A first aspect of the present invention generally relates to methods of prophylaxis or treatment of Stroke Related Disorders using various pharmaceutical compositions. A second aspect of the present invention generally relates to pharmaceutical compositions used for the prophylaxis or treatment of Stroke Related Disorders.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a ginkgo biloba extract, a preparation method and application of the ginkgo biloba extract in improvement of cerebral ischemia. The preparation method of the ginkgo biloba extract comprises the following steps: (1) crushing dried ginkgo biloba, and adding a compound enzyme aqueous solution for soaking to obtain a mixture 1; (2) adding a surfactant and absolute ethyl alcohol into the mixture 1, performing ultrasonic extraction, and filtering to obtain a ginkgo leaf extracting solution; and (3) filtering the ginkgo leaf extracting solution through a ceramic membrane, concentrating until the ginkgo leaf extracting solution has no ethanol smell, adding the ginkgo leaf extracting solution into a polyamide resin and macroporous resin combined resin column for elution, collecting eluent, concentrating and drying to obtain the ginkgo leaf extract. According to the ginkgo biloba extract, the specific compound enzyme, the surfactant and the purified resin are selected, and the ginkgo biloba extract obtained through extraction is high in effective component content and remarkable in cerebral ischemia improving effect.

A traditional Chinese medicine formula for treating blood vessel clogging belongs to the field of traditional Chinese medicine formulas, and comprises the following main raw materials: 29-31 parts of black pearl, 11-13 parts of codonopsis pilosula, 7-9 parts of saffron crocus, 8-10 parts of angelica sinensis, 190-210 parts of leek, 9-11 parts of pseudo-ginseng, 37-43 parts of hawthorn, 95-105 parts of astragalus membranaceus, 18-22 parts of kudzu vine root, 8-10 parts of peach kernel, 8-10 parts of prepared rehmannia root, 9-11 parts of radix rehmanniae, 11-13 parts of white paeony root, 3 parts of red ginseng, 9-11 parts of radix ophiopogonis, 14-16 parts of spina date seed, 11-13 parts of Chinese yam, 14-15 parts of schisandra chinensis, 9-11 parts of polygonum multiflorum, 7-9 parts of cinnamon, 18-21 , 13-15 parts of anise and 12-14 parts of rhizoma polygonati. The traditional Chinese medicine composition has an obvious improvement effect on coronary artery insufficiency, myocardial ischemia, mitral valve, tricuspid valve, aortic valve regurgitation, chronic heart failure, patients needing operation bypass stenting, angina pectoris, myocardial infarction sequelae, cerebral ischemia, cerebrovascular sequelae and other symptoms caused by cardiac insufficiency, the dosage is small, the effect is fast, the cure rate is high, relapse is not prone to occurring, and the traditional Chinese medicine composition is suitable for clinical application. The effects of tonifying qi and activating blood, dredging channels and relieving pain, eliminating phlegm and removing blood stasis, tonifying heart and soothing nerves and the like can be achieved.

The invention discloses application of miR-6340 in preparation of a medicine for treating cerebral ischemia. Metabolism of GM1 in MCAO / R rats and metabolism of ganglioside GM1 in microglial cells after OGD / R can be inhibited by down-regulating expression of Glb1. It is found that upstream miRNA (miR-6340) of a Glb1 protein coding gene and mir-6340 / Glb1 promote the autophagy process of microglial cells through metabolism of GM1. According to the application, a new view angle is provided for the neuroprotective effect of GM1 on OGD / R induction by reducing autophagy.

The invention provides a medicine for improving neurological dysfunction after cerebral ischemia, and relates to the technical field of biological medicine and molecular biology, and the medicine comprises the following steps: S1, preparing materials; s2, cell injury model and intervention test; s3, cell activity and oxidative stress index detection. The invention provides application of combination of baicalein and ferulic acid in preparation of a medicine for improving neurological dysfunction after cerebral ischemia, the embodiment of the invention proves that combination of baicalein and ferulic acid can improve neurological dysfunction after cerebral ischemia by reducing cerebral infarction area, resisting oxidation, inhibiting inflammatory response and the like, and test data shows that compared with a model group, the combination of baicalein and ferulic acid has the advantages that the combination of baicalein and ferulic acid has a good application prospect. White cerebral infarction areas of the rats in the administration group are reduced; after the palmitic acid treatment, the baicalein and ferulic acid combined administration group can promote the proliferation of SH-SY5Y nerve cells; after baicalein and ferulic acid are combined for treatment, the MDA level in damaged cells is remarkably reduced, and the SOD and GSH levels are remarkably increased, so that the baicalein and ferulic acid have the effects of resisting oxidation, inhibiting inflammation and protecting nerve cells.

The invention provides a traditional Chinese medicine preparation for cerebral arterial thrombosis, and belongs to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from gentiana macrophylla, divaricate saposhnikovia root, mulberry leaf, radix scrophulariae, ruddle, bulbus fritillariae cirrhosae, bamboo juice, selfheal, poria with hostwood and szechwan chinaberry fruit, and the whole formula has the effects of calming liver and suppressing yang, calming endogenous wind and relieving convulsion, eliminating phlegm and inducing resuscitation. The traditional Chinese medicine preparation has a remarkable improvement effect on the neurological function of a cerebral ischemia reperfusion injury model rat, can effectively reduce the water content of the brain tissue of the rat and the cerebral infarction volume, remarkably improves the levels of GSH and SOD in the brain tissue of the rat, reduces the MDA level, and has the effect of improving oxidative stress induced by cerebral ischemia reperfusion. The traditional Chinese medicine preparation can effectively relieve neurological function loss of a patient suffering from cerebral arterial thrombosis, help to recover life functions of the patient and relieve clinical symptoms of the patient, is definite and remarkable in curative effect and can be used as a new medicine for treating cerebral arterial thrombosis.

The invention discloses an aromatic alkylamine ferroptosis inhibitor based on a butylphthalide structure and a preparation method and application thereof. The chemical structural formula of the ferroptosis inhibitor is shown as a formula 1 or a formula 2. The ferroptosis inhibitor can inhibit ferroptosis caused by a ferroptosis inducer and can reduce the level of active oxygen in cells. Nerve injury caused by cerebral ischemia reperfusion can be relieved, and symptoms of neurological diseases such as Alzheimer's disease and Parkinson's disease can be relieved; compared with a ferroptosis inhibitor Ferrostatin-1, the aryl alkyl amine compound disclosed by the invention has better metabolic stability and is suitable for in-vivo drug effect evaluation. Therefore, the novel aryl alkyl amine compound provided by the invention has a very good application value in treatment of neurological diseases related to ferroptosis.

The present invention provides the use of a traditional Chinese medicine composition prepared from polygonum multiflorum, aloe, cassia seed, wolfberry fruit, donkey-hide gelatin, ginseng, atractylodes macrocephala and fructus aurantii immaturus as main components in the preparation of a drug for preventing or treating ischemic stroke. The research results show that Shouhui Laxative Capsule can reduce the neurological deficit score of cerebral ischemia-reperfusion injury, decrease the area of cerebral infarction, relieve cerebral pathological injury, and reduce the inflammatory level in serum, and has a significant brain protection effect on cerebral ischemia-reperfusion injury. The traditional Chinese medicine composition of the present invention has definite curative effect and safe components, and has broad application prospects and positive clinical significance.

The application discloses an indigo red derivative, a preparation method of the indigo red derivative, and application of the indigo red derivative in preparation of an anti-cerebral ischemia neuroprotective drug or a drug component. The indigo red derivative has good anti-cerebral ischemia neuroprotective activity, can overcome defects of existing active ingredients, such as easy bleeding, difficulty in penetrating a blood-brain barrier, poor oral bioavailability, low selectivity, and high neurobehavioral toxicity, and can be used as a cerebral neuroprotective active ingredient for treatment of ischemic cerebral stroke. According to an animal experiment of cerebral neuroprotective activity screening, it can be seen that after the compound is given preventively, the survival time of acute cerebral ischemia mice can be obviously prolonged, and the compound has good anti-cerebral ischemia neuroprotective effect.

The invention relates to a traditional Chinese medicine composition for treating sequelae caused by cerebral thrombosis or cerebral hemorrhage, the traditional Chinese medicine composition comprises 120-400 parts of Ligusticum wallichii, 200-450 parts of fried motherwort fruit, 100-450 parts of sandalwood and 2-10 parts of borneol, by mixing the raw materials according to a proper proportion, the traditional Chinese medicine composition can be used for treating sequelae caused by cerebral thrombosis and cerebral hemorrhage, and the traditional Chinese medicine composition can be used for treating sequelae caused by cerebral thrombosis and cerebral hemorrhage. The medicine has an outstanding treatment effect on diseases such as transient cerebral ischemia, central retinal vein occlusion and the like; the traditional Chinese medicine composition is simple in preparation method and suitable for large-scale production and popularization.

Disclosed are a hydroxypentyl benzoic acid diester compound, a method preparing the same, and an application of the same. This application uses a combination principle, on the one hand, the medicine concentration of butylphthalide in cerebral vessels is improved through a function of promoting the medicine to penetrate through a blood-brain barrier of dexborneol, and a multi-target multi-channel synergistic brain protection effect of the medicine is exerted; on the other hand, the treatment effect of dexborneol on cerebral ischemia is exerted. Anti-cerebral ischemia in-vivo pharmacodynamic activity evaluation is carried out on the synthesized compound, a high-activity candidate medicine is obtained, and industrial production can be achieved through the synthesis method.

The invention relates to the technical field of medicines, in particular to application of DP-b99 and a derivative thereof in preparation of a medicine for treating cerebral ischemia mechanical thrombus extraction reperfusion injury. On the basis of mechanical thrombus extraction and reperfusion of cerebral ischemia, DP-b99 is combined to reduce mechanical thrombus extraction and reperfusion injury of cerebral arterial thrombosis, so that the inflammation level of brain tissues can be reduced, the volume of cerebral infarction can be reduced, the injury of the brain tissues can be effectively reduced, and the treatment effect is remarkable.

The invention relates to the technical field of medicines, in particular to application of benzbromarone in preparation of antithrombotic drugs. The research finds that benzbromarone clinically used for treating hyperuricemia can be combined with HSP47 on platelets to inhibit the interaction between the HSP47 and collagen so as to reduce platelet activation and thrombosis, which prompts that benzbromarone is an HSP47 inhibitor with an anti-platelet effect; benzbromarone has the effects of inhibiting mouse common carotid artery thrombosis and relieving cerebral ischemia reperfusion injury. Experiments finally confirm that benzbromarone can inhibit formation of common carotid artery thrombosis, does not affect the blood coagulation function, is low in cytotoxicity, has the drug effect similar to that of aspirin and clopidogrel which are commonly used at present, and can become an effective and safe therapeutic drug for cerebral arterial thrombosis.

The invention provides a composition for relieving cerebral ischemia reperfusion injury as well as a preparation method and application thereof, and relates to the technical field of biological pharmacy. The composition comprises icariside I, squalene, an oil phase, an emulsifier and a co-emulsifier, the emulsifier is selected from one or a mixture of more of a nonionic surfactant, an anionic surfactant and an ampholytic surfactant; the particle size of the composition ranges from 50 nm to 500 nm. The icariside I and the squalene are mixed according to a proper proportion, and the oil phase, the emulsifying agent and the co-emulsifying agent are introduced, so that the solubility and the bioavailability of the icariside I are remarkably improved, and the drug effect is remarkably improved; meanwhile, the preparation method of the composition provided by the invention is simple in process and mild in condition, provides a universal method for preparing the hydrophobic drug microemulsion, is beneficial to large-scale production, and has wide application and market prospects.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a PSD-95 PDZ2 peptidomimetic inhibitor and application thereof. A series of compounds are designed and synthesized by taking a tetrapeptide ETAV as a template, a structure-activity relationship of the compounds with PSD-95 PDZ2 is systematically studied, and an optimal compound 32-2, i.e., the PSD-95 PDZ2 peptidomimetic inhibitor, is screened, a structural formula of which is shown as formula (I), the PSD-95 PDZ2 peptidomimetic inhibitor has very excellent plasma stability, and exhibits significant neuroprotective activity in in-vivo and in-vitro biological activity evaluation, significantly reduces cerebral infarction caused by cerebral ischemia-reperfusion injury, and can be used for preparing a drug for treating ischemic stroke. (I).

The invention belongs to the technical field of biological medicines, and particularly discloses a self-assembled nano preparation coated with a macrophage membrane as well as a preparation method and application of the self-assembled nano preparation. According to the nano preparation, nano particles formed by self-assembly of epigallocatechin gallate (EGCG), ferric chloride (FeCl3) and edaravone (EDV) serve as a core, and the outer layer of the nano preparation is coated with a macrophage membrane. The preparation method is a one-pot method and comprises the following steps: mixing and self-assembling EGCG, FeCl3 and EDV in a phosphate buffer solution, carrying out centrifugal purification, and carrying out ultrasonic fusion with a macrophage membrane. The nano preparation is uniform in particle size, has excellent free radical scavenging capacity and antioxidant activity, can effectively pass through a blood-brain barrier and target a cerebral ischemia reperfusion injury area, and shows a remarkable effect in preparation of drugs for treating cerebral arterial thrombosis.

The invention discloses a strain of lactobacillus acidophilus for treating chronic cerebral ischemia, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO: M 20251505. Animal experiments show that the strain can reduce the death rate of chronic cerebral ischemia model mice and improve neurological impairment, motion coordination and spatial learning and memory ability of the chronic cerebral ischemia model mice. Mechanism research shows that the strain can increase histone acetylation and BDNF secretion by inhibiting the activity of astrocyte HDAC1, and meanwhile, can inhibit an NF-kappa B signal channel and reduce inflammatory factor release by activating a microglial cell GPR120 receptor, so that a neuroprotective effect is achieved through an'intestine-brain axis'. The invention provides a new candidate strain for the development of microbial preparations for preventing and / or treating chronic cerebral ischemia and related cognitive impairment.