198 results about "Tetrapeptide" patented technology

The invention discloses tetrapeptide KA4 with an anti-fatigue function as well as a preparation method and application of the tetrapeptide KA4, and belongs to the technical field of biology. The tetrapeptide KA4 is screened from pacific salmon protamine enzymatic hydrolysate, the amino acid sequence is KYPA, and the tetrapeptide KA4 has strong binding capacity with KEAP1, AMPK and LDH, can significantly reduce the serum lactic acid content of a fatigue model mouse and significantly increase the load swimming time of the fatigue model mouse, has an anti-fatigue function, and can be applied to development of functional food for relieving fatigue.

The invention relates to the technical field of preparation methods of polypeptide drugs, and provides a solid-phase preparation method of tirpotide, which comprises the following steps: by taking amino resin as initial resin, sequentially coupling amino acid and small-fragment peptide according to an amino acid sequence of the tirpotide by using a solid-phase synthesis method to synthesize tirpotide resin; and cracking and purifying the tilpotide resin to obtain a pure tilpotide product. Wherein the small fragment peptide is 1-4 tetrapeptide fragment Boc-Tyr (tBu)-Aib-Glu (OtBu)-G1y-OH, 5-6 dipeptide fragment Fmoc-Thr (tBu)-Phe-OH, 7-8 dipeptide fragment Fmoc-Thr (tBu)-Ser (tBu)-OH, 10-11 dipeptide fragment Fmoc-Tyr (tBu)-Ser (tBu)-OH, 12-13 dipeptide fragment Fmoc-Ile-Aib-OH, 17-18 dipeptide fragment Fmoc-Lys (AEEA-AEA-gamma-Glu (alpha-OtBu)-Ecosanoic acid (mon-tBu), 20 side chain peptide Fmoc The coupling agent coupling the amino acid and the small fragment peptide is selected from the group consisting of Oxyma and DIC, TPTU and TMP or COMU and DIEA. The crude product of the tirpotide prepared by the method disclosed by the invention is high in yield and high in purity.

The invention provides an application of acetyl tetrapeptide-5 in preparation of an oil control product. According to the invention, the effect of acetyl tetrapeptide-5 on human sebaceous gland cells (SZ95) is researched through cell experiments. The compound reduces the lipid content.

The invention discloses a tetrapeptide LILP with uric acid reducing activity and application thereof, and belongs to the technical field of biological medicine. The amino acid sequence of the tetrapeptide LILP is Leuu-Ile-Leu-Pro, and the amino acid sequence of the tetrapeptide LILP is shown in the specification. The invention discloses that the tetrapeptide LILP has uric acid reducing activity for the first time, and uric acid generation can be reduced by reducing the activity of xanthine oxidase in the liver; uric acid discharge can be increased by reducing kidney uric acid reabsorption. The invention provides a multi-target active substance for reducing uric acid, which can be applied to preparation of medicines for preventing or treating hyperuricemia or preparation of health-care foods for assisting in reducing the level of uric acid, and has a good application prospect.

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

The invention provides an anti-aging repair composition containing PDRN, and lipid nanoparticles and application thereof. The composition provided by the invention is prepared from polydeoxyribonucleotide, micro-current tetrapeptide-1 and palmitoyl tripeptide-5. According to the application, the polydeoxyribonucleotide, the micro-current tetrapeptide-1 and the palmitoyl tripeptide-5 are compounded for use, so that the composition has a synergistic effect in the aspects of promoting cell proliferation, inhibiting an intracellular ROS (reactive oxygen species) level and the like, and has remarkable antioxidant, anti-inflammatory, anti-aging, soothing and repairing effects. The PDRN-containing composition is prepared into the lipid nanoparticles, so that the stability of the PDRN-containing composition is improved, the permeability of the PDRN-containing composition on the skin surface is improved, the bioavailability of the PDRN-containing composition is improved, the cell survival rate and cell uptake are promoted, the expression of adenosine A2A receptors, the synthesis of collagen, angiogenesis and the inhibition of anti-inflammatory factors are promoted, and a remarkable anti-aging effect is achieved.

The invention discloses a one-pot preparation method of semeglutide, and relates to the field of polypeptide synthesis. The preparation method comprises the following specific steps: 1) taking Arg34GLP-1 (9-37) as a starting material, controlling the pH value of a reaction solution, enabling activated dipeptide Fmoc-His-Aib-OH to react with the starting material to obtain an acylation reaction solution, and directly carrying out next-step reaction without aftertreatment after the reaction is finished; 2) adjusting the pH value of the acylation reaction liquid I, adding a compound 2-tetrapeptide into the acylation reaction liquid I, reacting to obtain acylation reaction liquid II, and directly carrying out next-step reaction without post-treatment after the reaction is finished; (3) adding an alkaline reagent to remove the Fmoc protecting group, settling, centrifuging and drying to obtain a crude product of the semeglutide; 4) separating and purifying the crude product to obtain a refined semeglutide product; the first three steps are carried out in the same reactor. Compared with a conventional liquid-phase synthesis method, the preparation method disclosed by the invention is simpler and more convenient, short in construction period, low in output of waste liquid and waste water, greatly reduced in cost, higher in yield and suitable for large-scale production.

The invention relates to a composite peptide with anti-inflammatory and soothing effects, a composition and application thereof, and belongs to the technical field of polypeptides. The invention relates to a composite peptide with anti-inflammatory and soothing effects. The composite peptide is prepared from the following components: tripeptide-1, acetyl dipeptide-1, palmitoyl tetrapeptide-7 and palmitoyl tripeptide-8, the mass ratio of the tripeptide-1 to the acetyl dipeptide-1 to the palmitoyl tetrapeptide-7 to the palmitoyl tripeptide-8 is (0.5 to 1.5) to (0.6 to 1.5) to (1 to 2) to (0.5 to 1.5). Experiments prove that the composite peptide has good anti-inflammatory, soothing and anti-wrinkle effects.

The invention provides a whole cortical action compound peptide, which comprises acetyl tetrapeptide-11, hexapeptide-9, palmitoyl hexapeptide-12, palmitoyl hexapeptide-14 and / or acetyl hexapeptide-1, and is characterized in that the whole cortical action compound peptide comprises acetyl tetrapeptide-11, hexapeptide-9, palmitoyl hexapeptide-12, palmitoyl hexapeptide-14 and / or acetyl hexapeptide-1; the compound peptide acts on epidermal basal layer keratinocytes, true epidermal junction (DEJ) structural proteins and / or dermal layer fibroblasts, and synergistically exerts a whole-cortex skin anti-aging effect on epidermal layer, basement membrane, dermal layer and / or nerve fibers. The compound peptide has the advantages of full-cortex three-dimensional anti-aging, layered precise targeting, multi-peptide scientific compounding, matrix synergy, clear action mechanism, efficient growth promotion of seven key proteins, remodeling of a true epidermis structure, low-concentration effect, mildness, no stimulation and the like, and is an advanced anti-aging scheme under a precise skin care trend.

Provided are an antibacterial peptide and the use thereof. The antibacterial peptide has a structure represented by formula (I), wherein Z1 is selected from tetrapeptides, the tetrapeptides being optionally modified by -OH, -CHO, -COOH, -NH2, -SO3H or -C=O; S1 and S2 are each independently selected from amino acids having the structure of (I); Q1 and Q2 are each independently selected from dipeptides or tripeptides; and n is independently selected from any integer between 1 and 6. (Q2)4-(S2-Q1)2-S1-Z1

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

The invention relates to transdermal tetrapeptide and application thereof, and belongs to the technical field of active polypeptide. A novel transdermal tetrapeptide Ala-Arg-Cys-Gly is designed and synthesized, the transdermal tetrapeptide has obvious penetration enhancing capacity, the penetration enhancing effect of the transdermal tetrapeptide is similar to that of the transdermal peptide TD-1 serving as an existing penetration enhancer and is remarkably superior to that of acetyl hexapeptide-1 and menthol serving as existing penetration enhancers, and the transdermal tetrapeptide is simple in structure, small in molecular weight, high in biocompatibility, free of toxic effect on cells and suitable for being used as a transdermal drug. The method can be used for preparing penetration promoting products and has wide development prospects and application potentials. Meanwhile, transdermal tetrapeptides Arg-Arg-Cys-Gly, Ala-Lys-Cys-Gly and Ala-Arg-Met-Gly are further synthesized, and although the transdermal delivery effect of the transdermal tetrapeptides is slightly lower than that of the Ala-Arg-Cys-Gly, the transdermal tetrapeptides still have an obvious penetration promoting effect.

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

The invention relates to a cyclic tetrapeptide derivative as well as a preparation method and application thereof. The structural formula of the cyclic tetrapeptide derivative is shown as a formula (I), and R is selected from C7-C19 alkyl and C7-C19 unsaturated chain alkyl. The cyclic tetrapeptide derivative or the salt or the stereoisomer thereof provided by the invention can improve the contents of hyaluronic acid, silk protein and alpha-crystal protein of epidermal cells, so that the skin barrier can be effectively enhanced, the obvious repairing and improving effects on the skin are achieved, and compared with palmitic acid tetrapeptide-10, the cyclic tetrapeptide derivative or the salt or the stereoisomer thereof has better stability and safety, and can be used for preparing a medicine for treating skin diseases. And when being used in cosmetics, the composition can play a better role in repairing and enhancing the skin barrier, and has a better improvement effect on the skin. # imgabs0 # (I)

The invention discloses a long-acting anti-aging composition capable of quickly removing dark circles and application of the composition. The composition is prepared from the following components: beta-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom-like peptide, arginine / lysine polypeptide, tocopheryl acetate and ergothioneine. According to the present invention, multiple functions are adopted, such that the effects of dark circle removing, repair, elasticity recovery, aging resistance and the like are provided so as to block the generation of dark circle, and the normal function of skin is ensured. The composition disclosed by the invention has the effect of quickly removing dark circles, and when the eye cream contains the composition, the eye cream can effectively remove the dark circles and has an obvious and quick effect within 15 minutes; after being used for a long time, the eye mask can remove dark circles and play a good control role.

The invention belongs to the technical field of cosmetics, and discloses a composition for promoting metabolism of hair papilla cells as well as a preparation method and application of the composition. The composition disclosed by the invention is prepared from the following components: a cacumen biotae extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4. The components in the composition have a good synergistic effect, the mitochondrial membrane potential of the hair papilla cells can be obviously enhanced, the cell activity of the hair papilla cells is promoted, the cell metabolism function of the hair papilla cells is promoted, and therefore the health of hair follicles is effectively maintained.

The invention relates to the field of biological medicines and cosmetics, in particular to a composition which takes tetrapeptide and bisabolol as core components and has anti-inflammatory and anti-aging activity.

The invention provides a hair care composition with oil control, hair loss prevention and repair effects and a preparation method of the hair care composition, and belongs to the technical field of hair care. The hair care composition comprises functional peptides such as myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 and the like which are synergistically combined according to a specific proportion, and glycerin, water and a penetration enhancer are supplemented to form a basic system. By optimizing peptide structure modification, adding proportion and the steps of pH control, ultrasonic treatment and collaborative assembly in the preparation process, multi-path collaborative regulation and control of hair follicle activation, scalp inflammation inhibition and keratin synthesis enhancement are realized.

The invention relates to the technical field of freeze-dried powder, in particular to a freeze-dried powder composition for hair regeneration and a preparation method thereof. The freeze-dried powder composition comprises 5-15 parts of a vascular endothelial growth factor, 3-10 parts of a platelet-derived growth factor, 1-5 parts of copper peptide, 0.5-3 parts of acetyl tetrapeptide-3, 2-8 parts of baicalein, 1-5 parts of asiaticoside, 20-40 parts of a freeze-drying protective agent and 1-3 parts of nano silicon dioxide. The preparation method comprises the following steps: mixing the pretreated vascular endothelial growth factor, platelet-derived growth factor, copper peptide, acetyl tetrapeptide-3, baicalein and asiaticoside with a freeze-drying protective additive and nano silicon dioxide, and freeze-drying. The freeze-dried powder composition can promote hair growth, improve scalp microenvironment and provide lasting support for healthy growth of hair. According to the preparation method, the stability and biological activity of the active ingredients are improved, and the overall performance and safety of the product are improved.

The invention relates to glassine ester as well as a preparation method and application thereof. The structural formula of the glassine ester is shown as a formula (I), each R1 is independently selected from hydrogen and-C (= O) R, and at least one R1 is-C (= O) R; r is selected from C7-C21 alkyl groups and C7-C21 unsaturated chain alkyl groups. The glassine ester has a remarkable DPPH free radical scavenging effect, can remarkably improve expression of elastin, hyaluronic acid and I-type collagen in cells, and has excellent effects of oxidation resistance, moisturizing and / or tightening and wrinkle resistance when being added into cosmetics as a functional component. # imgabs0 #

The invention belongs to the technical field of mesenchymal stem cell exosome compositions, and discloses a preparation method and application of a mesenchymal stem cell exosome composition. The mesenchymal stem cell exosome composition is prepared from a mesenchymal stem cell exosome, a hyaluronic acid-arginine grafted copolymer, acetyl tetrapeptide-5, resveratrol, mannose, polyglycerol-8 octoate and a buffer solution. Arginine residues contained in the hyaluronic acid-arginine graft copolymer in the composition can be specifically combined with integrin receptors (such as alpha v beta 3) highly expressed by fibroblasts at the wrinkle part, so that the exosome can actively anchor the wrinkle part, can be accurately and directionally enriched at the wrinkle part, and is high in utilization rate and high in anti-wrinkle efficiency. The composition constructs a three-dimensional anti-wrinkle network of mesenchymal stem cell exosome, acetyl tetrapeptide-5 and resveratrol, skin collagen degradation and oxidative damage are effectively restrained, and generation of skin wrinkles is blocked through multiple channels.

The invention relates to the technical field of polypeptide biosynthesis, in particular to a method for biosynthesizing acetyl tetrapeptide-5, a composition containing the acetyl tetrapeptide-5 and application of the composition. According to the method, low-cost natural amino acid is used as a substrate, specific aminopeptidase or a mutant thereof is used as a biocatalyst, amino acid ligase and polyphosphatase are combined, and acetyl tetrapeptide-5 can be efficiently synthesized through a one-step enzymatic reaction. Compared with a traditional chemical synthesis method, the method has the advantages of low raw material cost, no use of an organic solvent, mild reaction conditions, high regioselectivity, environmental friendliness, low production cost and the like. The invention also provides a composition containing acetyl tetrapeptide-5 and dipeptide-15, the inhibition effect of the composition on grease secretion is obviously better than that of a single component, and the composition has a synergistic oil control effect under a specific ratio.

Compositions comprising specific tetrapeptides, for use in methods for treating, preventing, minimizing, diminishing or reversing various signs of aging of the skin are provided. The compositions are useful in improving the firmness or elasticity of skin, smoothing of fine-lines or wrinkles, reducing skin pores and hyperpigmentation, and increasing skin thickness, radiance and / or softness.

This invention belongs to the fields of biomedicine, beauty, and personal care health technology, and discloses a dual-target oil-controlling peptide co-amorphous assembly, its preparation method, and its application. The method involves adding capryloylglycine and acetyl tetrapeptide-5 to an alcohol solution, heating to dissolve, rotary evaporating, and drying to obtain a co-amorphous compound. This compound is then mixed with a cyclodextrin compound and water, rotary evaporating, and drying to obtain the dual-target oil-controlling peptide co-amorphous assembly. This invention employs a co-amorphous combination with cyclodextrin inclusion to assemble acetyl tetrapeptide-5 and capryloylglycine, solving the problem of their large solubility differences and difficulty in coexistence, and significantly improving the solubility of capryloylglycine. This assembly enables simultaneous transdermal delivery of the two active components through nano-assembly, helping to enhance the stability of acetyl tetrapeptide-5 and reduce its oxidative degradation. The two components synergistically control oil through a dual pathway of inhibiting 5α-reductase and targeting the MC5R receptor, respectively, exhibiting superior bioavailability and efficacy compared to a single component.

The invention discloses a polypeptide composition for promoting skin desquamation and a preparation method and application thereof, the polypeptide composition comprises polypeptides and a polyol preservative, the polypeptides comprise a first polypeptide, a second polypeptide and a third polypeptide, and the first polypeptide is acetyl tetrapeptide-2; the second polypeptide comprises one or more of hyaluronic acid polypeptide, red scorpion toxin and palmitoyl tripeptide-5; and the third polypeptide is prepared from one or more of acetyl octapeptide-3, palmitoyl dipeptide-7, acetyl tetrapeptide-9 and acetyl tetrapeptide-11. The polypeptide composition can significantly up-regulate the gene expression of kallikrein through a synergistic effect and promote old cutin exfoliation; meanwhile, gene expression of metalloproteinase is inhibited, and light aging is delayed. The polypeptide composition disclosed by the invention can be prepared into various cosmetics and has multiple effects of promoting skin desquamation, resisting wrinkles and repairing barriers.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

Compositions comprising specific tetrapeptides, for use in treating, preventing, minimizing, diminishing or reversing various signs of aging of the skin are provided. The compositions are useful in improving the firmness or elasticity of skin, smoothing of fine-lines or wrinkles, reducing skin pores and hyperpigmentation, and increasing skin thickness, radiance and / or softness.

The application discloses derivatives of pyroglutamic acid-tetrapeptide-7 and application thereof. The derivatives of pyroglutamic acid-tetrapeptide-7 or pharmaceutically acceptable salts thereof have the following structure: the substituent R is selected from the group consisting of. The derivatives of pyroglutamic acid-tetrapeptide-7 have excellent effects of anti-inflammation, promoting synthesis of collagen and / or elastin, reducing wrinkle area or wrinkle degree, and have a smaller effective concentration compared with tetrapeptide-7, and can be applied to medicines, cosmetics or skin care products as main active ingredients.

The application discloses a tetrapeptide IR4 with uric acid reducing activity, and a preparation method and application thereof, and belongs to the technical field of small molecular peptides. The amino acid sequence of the tetrapeptide IR4 is IDWR, and the tetrapeptide IR4 can be prepared by a solid-phase synthesis method and an enzymatic hydrolysis method. The tetrapeptide IR4 is identified from a porphyra haitanensis protease hydrolysate, has potential interaction with xanthine oxidase, and can reduce the uric acid content of zebrafish by 33.6% (to 5.93+ / -0.47 mu mol / g protein) at a concentration of 100 mu g / mL. Compared with an anserine (which can reduce the uric acid content of zebrafish by 25.7%), the tetrapeptide IR4 has better uric acid reducing activity. Compared with allopurinol (which can reduce the uric acid content of zebrafish to 4.61+ / -1.11 mu mol / g protein), the uric acid reducing ability of the tetrapeptide IR4 and the allopurinol has no significant difference. The tetrapeptide IR4 can be used for preparing uric acid reducing drugs and relieving hyperuricemia.

Pharmaceutical compositions containing specific tetrapeptides for use in treating, preventing, minimizing, diminishing or reversing degenerative, age-related, or trauma-induced diseases or disorders in a subject in need thereof. The disease may be a disease associated with ROS-related oxidative stress. The tetrapeptides may have an amino acid sequence of pGlu-Asn-Trp-Lys(Octanoyl)-OH as set forth in SEQ ID NO: 1 or pGlu-Asn-Trp-Thr-OH as set forth in SEQ ID NO: 2, preferably as a sodium salt thereof.