135 results about "Tetramethylpyrazine" patented technology

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

The invention relates to the technical field of traditional Chinese medicine carbon dots, in particular to ligusticum wallichii carbon dots, a medicine and application. The ligusticum wallichii carbon dots disclosed by the invention are obtained by carbonizing ligusticum wallichii by a hydrothermal method, wherein the hydrothermal method carbonization is a hydrothermal reaction at 175-195 DEG C for 8-12 hours; research finds that the ligusticum wallichii carbon dots have the characteristic of treating myocardial apoptosis caused by myocardial ischemia reperfusion injury, and compared with ligusticum wallichii carbon dots synthesized by ligustrazine and a pyrolysis method, the ligusticum wallichii carbon dots have the advantage that the heart function can be better recovered. The invention provides a new candidate drug for treating myocardial ischemia reperfusion injury.

The invention relates to the technical field of traditional Chinese medicine pharmacy, and discloses a traditional Chinese medicine micro-powder capsule based on functions of promoting blood circulation to remove blood stasis, dispelling wind and eliminating dampness and activating meridians to stop pain. 0.8%-1.2% of scorpion; 0.8%-1.2% of Chinese polyphaga; 0.8%-1.2% of safflower carthamus; 0.8%-1.2% of peach kernels; 0.8%-1.2% of zaocys dhumnade; 0.8% to 1.2% of frankincense; 0.8%-1.2% of ligusticum wallichii; 0.8%-1.2% of cortex moutan; 0.8% to 1.2% of myrrh; 0.8%-1.2% of notopterygium root; 0.8%-1.2% of radix angelicae pubescentis; 0.8%-1.2% of fructus lycii; 0.8%-1.2% of centipede; 15%-25% of a functional auxiliary material: 3%-5% of a nano dispersion carrier; and 3%-5% of an inclusion compound. By adopting a beta-cyclodextrin inclusion technology, volatile oil and a humid and hot environment can be effectively isolated, the stability of the volatile oil can be enhanced, by taking ligustrazine as an example, under an accelerated test condition, the retention rate of the ligustrazine in 6 months is up to 89.5%, and compared with a non-inclusion condition, the retention rate is improved, the degradation rate is reduced by 3.1 times, and the stability of the core component for promoting blood circulation to remove blood stasis in the storage and use process is ensured.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a new enterobacter rorrhizae strain as well as a separation and screening method and application thereof. In order to enrich microbial resources for producing pyrazine substances through metabolism in the white spirit industry, an enterobacter rorrhizae strain is separated and screened from a mixture of yeast for making hard liquor and fermented grains, and the preservation number is CGMCC No.33262. The strain has good tolerance to pH, salt and alcohol, and can adapt to the growth environment in the white spirit production process; and various pyrazine substances represented by tetramethylpyrazine can be produced. Besides, the strain has strong adaptability to fermented grains, the yield of tetramethylpyrazine is high in a culture medium taking bran, wheat and five grains as the fermented grains, and meanwhile, various flavor substances such as ethyl acetate, ethyl hexanoate, ethyl caprylate, 2, 3-butanediol, guaiacol and 4-vinyl guaiacol can be produced. Therefore, the enterobacter rorrhizae disclosed by the invention can be widely used for brewing white spirit.

The invention belongs to the technical field of medicinal chemistry, and provides an etodolac-tetramethylpyrazine eutectic and a preparation method thereof.The etodolac-tetramethylpyrazine eutectic is characterized in that a basic unit of the eutectic is composed of two molecules of etodolac and one molecule of tetramethylpyrazine, Cu-K alpha radiation is used, and the etodolac-tetramethylpyrazine eutectic is obtained. An X-ray diffraction spectrum represented by 2theta has characteristic peaks at least at 9.4 + / -0.2 degrees, 13.7 + / -0.2 degrees, 14.6 + / -0.2 degrees, 18.8 + / -0.2 degrees, 27.3 + / -0.2 degrees and 27.7 + / -0.2 degrees. The prepared etodolac-tetramethylpyrazine eutectic is high in purity, has better chemical stability and solubility, and is beneficial to storage and transportation of products and application in preparation of preparations.

This disclosure herein describes the use of cannabidiol (CBD) with tetramethylpyrazine (TMP) to improve the bioavailability and efficacy of CBD. Behavioral studies indicate that the oral administration of CBD with TMP has a strong anxiolytic effect, increases social behavior in stressed rats, and alleviates anhedonia-like symptoms in stressed rats. These effects were significantly greater than observed with CBD alone, most likely due to increased CBD exposure with TMP administration and / or a metabolism effect of the co-administration of CBD and TMP.

The invention discloses a traditional Chinese medicine composition for treating chronic obstructive pulmonary disease as well as a preparation and application thereof, and belongs to the technical field of chronic obstructive pulmonary disease medicines. The traditional Chinese medicine composition consists of a qi tonifying component, a blood activating component and a phlegm reducing component, or consists of the qi tonifying component and the blood activating component, or consists of the qi tonifying component and the phlegm reducing component, wherein the qi-tonifying component is selected from any one or a combination of more of ginsenoside Rg1, ginsenoside Rb1, astragaloside and schizandrin; the blood activating component is selected from any one or combination of more of ligustrazine and ferulic acid; the phlegm reducing component is selected from any one or combination of more of glucosinolate and quercetin. The compatibility and combination of the medicines have obvious effects in the aspects of improving lung inflammatory response and inhibiting pathological remodeling of pulmonary blood vessels.

The invention provides a preparation method and application of ligustrazine and salts thereof. According to the method disclosed by the invention, a mode of dropwise adding part of reaction materials is adopted, so that the phenomenon of rapid temperature rushing in the preparation of ligustrazine is avoided, the risks of material spraying and explosion of a reaction system in large-scale production are avoided, and the personal safety is effectively protected. In addition, the method provided by the invention has the advantages of simple and easily available raw materials, low price, short reaction time, high total yield, good product purity, reduction of three wastes, energy conservation and environmental protection, and is especially suitable for large-scale production.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an ambroxol hydrochloride-containing oral liquid and application thereof, and the ambroxol hydrochloride oral liquid is composed of ambroxol hydrochloride, ligustrazine, meglumine, dextran 40, xylitol, carboxymethylcellulose, L-tryptophan, stevioside and purified water. The ligustrazine and the ambroxol hydrochloride play a synergistic effect, and the meglumine and the dextran 40 are combined for use, so that the stability of the ambroxol hydrochloride is improved. Accelerated experiments show that the ambroxol hydrochloride is stable in property and suitable for further industrial mass production.

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a strain of Kroppenstedtii ivory color and application thereof. In order to provide more high-temperature-resistant and pyrazine-producing brewing microbial resources, a new strain of Kroppenstedtii ivory color MZ35 is isolated from Maotai-flavor Daqu, and the preservation number is CCTCC NO: M 20252598. The strain can adapt to the solid-state fermentation environment of higher temperature and the pile fermentation environment of Maotai-flavor liquor, and promote the formation of tetramethylpyrazine. After MZ35 is inoculated into the solid-state fermentation system of highland barley or the intensified fermentation system of wine lees, the content of tetramethylpyrazine can be significantly increased. In addition, the strain can also promote the accumulation of acetoin, trimethylpyrazine and other Maotai-flavor related substances or precursor substances. Therefore, the strain has important application value for the pile fermentation of liquor, the flavor regulation of wine lees and the preparation of solid-state fermentation products rich in tetramethylpyrazine.

The present invention belongs to the technical field of fermentation engineering, and specifically discloses a strain of Moutai slow-growing Bacillus (Lentibacillus maotaii) JN110522, which is stored in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a preservation date of June 29, 2022, and a preservation number of CGMCC No.25214. The strain can simultaneously metabolize and produce high levels of 2,3,5,6-tetramethylpyrazine and 2,3,5-trimethylpyrazine, and the output of 2,3,5,6-tetramethylpyrazine can reach 26.2±0.8mg / L, and the content of 2,3,5-trimethylpyrazine can reach 4.1±0.4mg / L. The present invention also discloses that the strain is applied to the compression stage of high-temperature Daqu fermentation process, which can greatly increase the content of 2,3,5,6-tetramethylpyrazine and 2,3,5-trimethylpyrazine in high-temperature Daqu, so that the sauce-flavored flavor of high-temperature Daqu is better prominent, and can be used to prepare high-performance Daqu. The total yield of pyrazine compounds, 2,3,5,6-tetramethylpyrazine and 2,3,5-trimethylpyrazine of the fortified qu fermented by this bacterium is 1 times higher than that of ordinary Maotai slow-growing Bacillus fortified qu, and the application value is very high.

The present invention relates to the use of ligustrazine nitrone derivatives and pharmaceutical composition thereof for the treatment and prevention of diseases of diabetic complications, which include renal anemia. The ligustrazine nitrone derivatives have the structure of the general formula (I).Experimental results indicated that the ligustrazine nitrone derivatives can significantly increase the levels of serum iron and erythropoietin in STZ induced SD rats and spontaneously hypertensive rats, and can be used for treating and preventing renal anemia caused by decrease in renal EPO production and / or concomitant iron deficiency. Therefore, the ligustrazine nitrone derivatives can be made into various dosage forms with a drug carrier. The derivatives can be prepared into various dose forms together with drug carriers.

This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The application discloses a NO donor type ligustrazine derivative, a preparation method, a composition and application, and provides a NO donor type ligustrazine derivative and a preparation method thereof, and further provides a composition of different dosage forms prepared by mixing the NO donor type ligustrazine derivative or a pharmaceutically acceptable salt thereof and a pharmaceutical excipient, and application of the composition in preparation of a medicine for preventing or treating thromboembolic cardiovascular and cerebrovascular diseases and diseases caused by platelet aggregation.

The invention relates to a preparation method of tetramethylpyrazine, which is characterized in that 3-hydroxy-2-butanone and ammonium acetate are used as raw materials, a potassium hydrogen persulfate compound (Oxone) is used as an oxidizing agent, and 2, 3, 5, 6-tetramethylpyrazine is prepared through reaction. The preparation method has the beneficial effects that the reaction is rapid, the condition is mild, an organic solvent is not needed, and industrial popularization is easy; the color of a reaction system solution in the reaction process is light, and the reaction solution can be used mechanically, so that the green, environment-friendly and safe production and purification processes of the product are realized; the prepared product can be directly obtained in a filtering mode, and the product is high in purity, good in appearance, high in yield and suitable for one-pot production.

The invention discloses ligustrazine ferulate and a preparation method and application thereof, and belongs to the technical field of pharmacy, the preparation method comprises the following steps: dissolving ligustrazine in methanol to obtain a methanol solution of the ligustrazine, weighing the ligustrazine, adding the ligustrazine into a bottle, magnetically stirring at 28 DEG C for 24 hours, controlling the water bath temperature at 50 DEG C, and performing vacuum concentration and suction filtration to obtain the ligustrazine ferulate. The ligustrazine ferulate provided by the invention is higher in solubility, and can play a better anti-pulmonary fibrosis drug effect.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The invention discloses bacillus subtilis for producing tetramethylpyrazine and application of the bacillus subtilis in baijiu, and relates to the field of baijiu brewing and food safety. The preservation number of the bacillus subtilis is CCTCC NO: M20241287, and the bacillus subtilis can be applied to preparation of tetramethylpyrazine and / or fermented products, such as Baijiu, and can remarkably improve the content of tetramethylpyrazine and the content of esters in the Baijiu and reduce the content of methyl mercaptan, so that the quality of the Baijiu is improved, the flavor of the Baijiu is optimized, and the quality of the Baijiu is improved. And a new way is provided for production of tetramethylpyrazine and preparation of high-quality white spirit.

The invention discloses a preparation method of composite roasted peanut essence, which belongs to the technical field of essence, and comprises the following steps: preheating peanut oil and caprylic / capric triglyceride; the preparation method comprises the following steps: dissolving ethyl maltol, vanillin, methyl cyclopentenolone, 2, 3, 5-trimethylpyrazine and 2-acetylpyrazine; vanillin propylene glycol acetal, isovaleraldehyde propylene glycol acetal, triethyl citrate, delta-decalactone, delta-dodecalactone and 5-hydroxyethyl-4-methylthiazole are fused to obtain a mixture; dispersing the roasted peanut extract; 2, 3-dimethylpyrazine, 2-methoxyl-5-methylpyrazine and 4-methylthiazole are fused, and then the mixture is subjected to a reaction; the preparation method comprises the following steps: fusing cocaal, dimethyl sulfide, 2-ethyl-3, 5, 6-dimethylpyrazine, 2, 3, 5, 6-tetramethylpyrazine, 2-methylpyrazine, 2-acetylpyridine, 2-methoxy-3. 5. 6-isopropyl pyrazine, 2-methylthio-3-methylpyrazine, isovaleric acid, 1-octylene-3-alcohol, 5-methylfurfural and 3, 4-dimethyl-1, 2-cyclopentanedione; standing is performed; filtering is performed; the fragrance of the roasted peanuts is closer to that of the roasted peanuts which are just taken out of a furnace, and the natural flavor of the roasted peanut essence is increased.

The present invention provides use of a ligustrazine nitrone derivative in the preparation of a drug for preventing or treating sarcopenia. The ligustrazine nitrone derivative of the present invention can effectively improve the muscle mass, muscle strength, and muscle functions of sarcopenia model animals, and has the potential to be developed into the drug for preventing or treating sarcopenia. The ligustrazine nitrone derivative has a structure represented by general formula (I) below.

The present application relates to the field of microbial fermentation and bioengineering technology, and particularly relates to bacillus licheniformis and application thereof in acetoin and tetramethylpyrazine production. A bacillus licheniformis with high acetoin yield is screened from a large number of strains. The acetoin yield of the strain can reach 98 g / L after 36 h fermentation, which is much higher than that of other natural screening strains, and the liquor yield is increased by 11% and the content of tetramethylpyrazine in the liquor is increased by 57% when the strain is used for liquor brewing, which obviously improves the quality of the liquor.

The application relates to the technical field of pesticides, and particularly discloses a tetramethylpyrazine suspension agent with slow-release performance and a preparation method thereof. The tetramethylpyrazine is prepared into a suspension, so that the particle size of the tetramethylpyrazine can be controlled, and tetramethylpyrazine particles with a suitable particle size as a capsule core can be obtained without dissolving and emulsifying the tetramethylpyrazine technical material. The application overcomes the defect that the tetramethylpyrazine is difficult to adopt a traditional microcapsule coating process, and a capsule membrane formed by a melamine modified urea-formaldehyde resin prepolymer can hinder the degradation of the tetramethylpyrazine, thereby prolonging the persistence period of the tetramethylpyrazine suspension agent and improving the insecticidal effect of the tetramethylpyrazine pesticide.

The invention relates to the technical field of scar repair medicines, in particular to compound scar traditional Chinese medicine microemulsion gel which is composed of traditional Chinese medicine functional components, microemulsion components, gel matrix components and auxiliary materials. The traditional Chinese medicine functional components comprise tanshinone IIA, asiaticoside, an angelica sinensis extract, dipotassium glycyrrhizinate and ligustrazine; the microemulsion component comprises an oil phase, an emulsifier, a co-emulsifier and a water phase, the emulsifier is a compound of polyoxyethylene hydrogenated castor oil EL-40 and Tween 80, and the co-emulsifier comprises 1, 2-propylene glycol; the gel matrix component is prepared from carbomer 940, hydroxypropyl methyl cellulose, triethanolamine and purified water; the auxiliary materials comprise phenoxyethanol and sodium hyaluronate. According to the invention, a scientific traditional Chinese medicine compound system is constructed, and a plurality of functional components have a synergistic effect and respectively play the roles of resisting inflammation, inhibiting fibroblast proliferation, promoting tissue repair, improving local blood circulation and the like.

The invention discloses a tobacco leaf fermentation and quality improvement method based on oxygen content regulation and application thereof, and the method comprises the following steps: preparing a tobacco culture medium, crushing tobacco leaves, sieving to obtain tobacco powder, mixing the tobacco powder with water in proportion, extracting, filtering and sterilizing to obtain the tobacco leaf fermentation and quality improvement method; respectively culturing Klebsiella HNYJ-1 single colonies and enterobacter HNYJ-4 single colonies to obtain seed solutions, and mixing the seed solutions in proportion to obtain a composite seed solution; and finally, after the tobacco leaves are moistened in a redrying stage and before redrying, spraying the composite seed solution, firstly carrying out aerobic fermentation in a closed temporary storage cabinet for 12 hours, then adjusting the oxygen to 5-20%, carrying out low-oxygen fermentation for 24 hours, and after fermentation, redrying at high temperature to inactivate the strain. Compared with unfermented and single aerobic / hypoxic fermentation, the method has the advantages that the content of linalool, 2, 3, 5, 6-tetramethylpyrazine and other key aroma components can be greatly increased, the aroma quality, the aroma amount and the fineness of the tobacco leaves are remarkably improved, offensive odor and irritation are reduced, the process is adaptive to an existing processing flow, and extra complex equipment is not needed.

The invention discloses a plaster for treating cervical spondylosis and a preparation method thereof, and relates to the technical field of traditional Chinese medicine external preparations. According to the present invention, with the application of the targeted transdermal system, the transdermal rate of the ligustrazine and other active components can be increased to 68-75% through the experimental verification by using the borneol + menthol + azone / propylene glycol composite permeation enhancing agent, and the traditional Chinese medicine such as radix aconiti, radix aconiti agrestis, garden balsam stem and the like is subjected to gradient extraction, and is combined with the borneol / menthol / azone composite permeation enhancing system and the medical gel matrix to prepare the target transdermal system; according to the plaster, the medicine is directionally conveyed to diseased tissues through the skin, the liver and kidney toxicity problem of traditional oral medicine is avoided, the transdermal absorption rate is increased to 70% or above, and the plaster has the triple effects of resisting inflammation, easing pain and improving microcirculation and is suitable for long-term treatment of nerve root type and neck type cervical spondylosis.

The invention belongs to the field of medicinal chemistry, and particularly relates to ligustrazine stilbene polyphenol aromatic acid ether compounds, pharmaceutically acceptable salts thereof, a preparation method thereof, a pharmaceutical composition and an application of the ligustrazine stilbene polyphenol aromatic acid ether compounds. The ligustrazine stilbene polyphenol aromatic acid ether compound and the pharmaceutically acceptable salt thereof have a remarkable anti-platelet aggregation effect, and can be used for preparing drugs for resisting platelet aggregation, preventing or treating cardiovascular diseases or treating or preventing thromboembolic diseases.

The invention belongs to the technical field of functional food, and particularly relates to a compound oral liquid beneficial to maintaining the healthy level of blood pressure and a preparation method thereof.The compound oral liquid is prepared from, by weight, 4-12 parts of Shanxi mature vinegar, 3-12 parts of folium cortex eucommiae and 3-12 parts of folium apocyni veneti. The traditional Chinese medicine composition contains various antihypertensive active substances such as melanoidins and ligustrazine, two traditional Chinese medicinal materials such as folium cortex eucommiae and folium apocyni veneti which have an antihypertensive effect are matched, the traditional Chinese medicinal materials are rich in antihypertensive components such as flavonoid and phenolic acid, the optimal proportion is obtained through multiple tests, and the efficacy of cooperatively promoting and maintaining the blood pressure health level is achieved. A series of complications caused by hypertension are relieved, the healthy level of blood pressure can be maintained and tends to be stable, and the three components have a remarkable synergistic interaction effect according to the prescription range and the preparation method disclosed by the patent.

The application provides a triazole compound for protecting brain tissue and a preparation method thereof, and belongs to the technical field of biological medicines, and provides a triazole compound as shown in formula (I) and chiral isomers, enantiomers, diastereoisomers, geometric isomers, free forms or pharmaceutically acceptable salts, hydrates, solvates or esters thereof; and also provides a preparation method thereof; compared with the prior art, the application combines the internal ester ring-opening product of butylphthalide and ligustrazine through triazole to synthesize a novel triazole compound which can be used for protecting brain tissue; the preparation method of the triazole compound is simple and the raw materials are easy to obtain; the triazole compound has better biological activity and brain tissue protection effect than butylphthalide / ligustrazine, and has significant protection activity on a SH-SY5Y cell damage model.

The invention discloses a tendon-dredging and bone-penetrating health-care liquid and a preparation method thereof. According to the preparation, erycibe obtusifolia benth and radix aconiti preparata are taken as cores, the compatibility design of detoxification of liquorice and synergism of cassia twig is combined with a beta-cyclodextrin inclusion technology, so that the content of aconitine is smaller than or equal to 0.02 mg / mL, and the transdermal rate of ligustrazine is larger than or equal to 65%. The preparation process comprises three modules of dynamic circulation extraction, membrane separation refining and sterile filling, so that the retention rate of volatile components is greater than 85%. Animal experiments prove that the onset time of the analgesic effect is shortened by 40% compared with that of similar products, and the traditional Chinese medicine composition is suitable for conditioning sub-health states such as joint flexion and extension disadvantage.