72 results about "Tetramethylpyrazine" patented technology

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a strain of Kroppenstedtii ivory color and application thereof. In order to provide more high-temperature-resistant and pyrazine-producing brewing microbial resources, a new strain of Kroppenstedtii ivory color MZ35 is isolated from Maotai-flavor Daqu, and the preservation number is CCTCC NO: M 20252598. The strain can adapt to the solid-state fermentation environment of higher temperature and the pile fermentation environment of Maotai-flavor liquor, and promote the formation of tetramethylpyrazine. After MZ35 is inoculated into the solid-state fermentation system of highland barley or the intensified fermentation system of wine lees, the content of tetramethylpyrazine can be significantly increased. In addition, the strain can also promote the accumulation of acetoin, trimethylpyrazine and other Maotai-flavor related substances or precursor substances. Therefore, the strain has important application value for the pile fermentation of liquor, the flavor regulation of wine lees and the preparation of solid-state fermentation products rich in tetramethylpyrazine.

This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The application discloses a NO donor type ligustrazine derivative, a preparation method, a composition and application, and provides a NO donor type ligustrazine derivative and a preparation method thereof, and further provides a composition of different dosage forms prepared by mixing the NO donor type ligustrazine derivative or a pharmaceutically acceptable salt thereof and a pharmaceutical excipient, and application of the composition in preparation of a medicine for preventing or treating thromboembolic cardiovascular and cerebrovascular diseases and diseases caused by platelet aggregation.

The invention relates to a preparation method of tetramethylpyrazine, which is characterized in that 3-hydroxy-2-butanone and ammonium acetate are used as raw materials, a potassium hydrogen persulfate compound (Oxone) is used as an oxidizing agent, and 2, 3, 5, 6-tetramethylpyrazine is prepared through reaction. The preparation method has the beneficial effects that the reaction is rapid, the condition is mild, an organic solvent is not needed, and industrial popularization is easy; the color of a reaction system solution in the reaction process is light, and the reaction solution can be used mechanically, so that the green, environment-friendly and safe production and purification processes of the product are realized; the prepared product can be directly obtained in a filtering mode, and the product is high in purity, good in appearance, high in yield and suitable for one-pot production.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The invention discloses a preparation method of composite roasted peanut essence, which belongs to the technical field of essence, and comprises the following steps: preheating peanut oil and caprylic / capric triglyceride; the preparation method comprises the following steps: dissolving ethyl maltol, vanillin, methyl cyclopentenolone, 2, 3, 5-trimethylpyrazine and 2-acetylpyrazine; vanillin propylene glycol acetal, isovaleraldehyde propylene glycol acetal, triethyl citrate, delta-decalactone, delta-dodecalactone and 5-hydroxyethyl-4-methylthiazole are fused to obtain a mixture; dispersing the roasted peanut extract; 2, 3-dimethylpyrazine, 2-methoxyl-5-methylpyrazine and 4-methylthiazole are fused, and then the mixture is subjected to a reaction; the preparation method comprises the following steps: fusing cocaal, dimethyl sulfide, 2-ethyl-3, 5, 6-dimethylpyrazine, 2, 3, 5, 6-tetramethylpyrazine, 2-methylpyrazine, 2-acetylpyridine, 2-methoxy-3. 5. 6-isopropyl pyrazine, 2-methylthio-3-methylpyrazine, isovaleric acid, 1-octylene-3-alcohol, 5-methylfurfural and 3, 4-dimethyl-1, 2-cyclopentanedione; standing is performed; filtering is performed; the fragrance of the roasted peanuts is closer to that of the roasted peanuts which are just taken out of a furnace, and the natural flavor of the roasted peanut essence is increased.

The present application relates to the field of microbial fermentation and bioengineering technology, and particularly relates to bacillus licheniformis and application thereof in acetoin and tetramethylpyrazine production. A bacillus licheniformis with high acetoin yield is screened from a large number of strains. The acetoin yield of the strain can reach 98 g / L after 36 h fermentation, which is much higher than that of other natural screening strains, and the liquor yield is increased by 11% and the content of tetramethylpyrazine in the liquor is increased by 57% when the strain is used for liquor brewing, which obviously improves the quality of the liquor.

The application relates to the technical field of pesticides, and particularly discloses a tetramethylpyrazine suspension agent with slow-release performance and a preparation method thereof. The tetramethylpyrazine is prepared into a suspension, so that the particle size of the tetramethylpyrazine can be controlled, and tetramethylpyrazine particles with a suitable particle size as a capsule core can be obtained without dissolving and emulsifying the tetramethylpyrazine technical material. The application overcomes the defect that the tetramethylpyrazine is difficult to adopt a traditional microcapsule coating process, and a capsule membrane formed by a melamine modified urea-formaldehyde resin prepolymer can hinder the degradation of the tetramethylpyrazine, thereby prolonging the persistence period of the tetramethylpyrazine suspension agent and improving the insecticidal effect of the tetramethylpyrazine pesticide.

The invention relates to the technical field of scar repair medicines, in particular to compound scar traditional Chinese medicine microemulsion gel which is composed of traditional Chinese medicine functional components, microemulsion components, gel matrix components and auxiliary materials. The traditional Chinese medicine functional components comprise tanshinone IIA, asiaticoside, an angelica sinensis extract, dipotassium glycyrrhizinate and ligustrazine; the microemulsion component comprises an oil phase, an emulsifier, a co-emulsifier and a water phase, the emulsifier is a compound of polyoxyethylene hydrogenated castor oil EL-40 and Tween 80, and the co-emulsifier comprises 1, 2-propylene glycol; the gel matrix component is prepared from carbomer 940, hydroxypropyl methyl cellulose, triethanolamine and purified water; the auxiliary materials comprise phenoxyethanol and sodium hyaluronate. According to the invention, a scientific traditional Chinese medicine compound system is constructed, and a plurality of functional components have a synergistic effect and respectively play the roles of resisting inflammation, inhibiting fibroblast proliferation, promoting tissue repair, improving local blood circulation and the like.

The invention discloses a tobacco leaf fermentation and quality improvement method based on oxygen content regulation and application thereof, and the method comprises the following steps: preparing a tobacco culture medium, crushing tobacco leaves, sieving to obtain tobacco powder, mixing the tobacco powder with water in proportion, extracting, filtering and sterilizing to obtain the tobacco leaf fermentation and quality improvement method; respectively culturing Klebsiella HNYJ-1 single colonies and enterobacter HNYJ-4 single colonies to obtain seed solutions, and mixing the seed solutions in proportion to obtain a composite seed solution; and finally, after the tobacco leaves are moistened in a redrying stage and before redrying, spraying the composite seed solution, firstly carrying out aerobic fermentation in a closed temporary storage cabinet for 12 hours, then adjusting the oxygen to 5-20%, carrying out low-oxygen fermentation for 24 hours, and after fermentation, redrying at high temperature to inactivate the strain. Compared with unfermented and single aerobic / hypoxic fermentation, the method has the advantages that the content of linalool, 2, 3, 5, 6-tetramethylpyrazine and other key aroma components can be greatly increased, the aroma quality, the aroma amount and the fineness of the tobacco leaves are remarkably improved, offensive odor and irritation are reduced, the process is adaptive to an existing processing flow, and extra complex equipment is not needed.

The invention belongs to the field of medicinal chemistry, and particularly relates to ligustrazine stilbene polyphenol aromatic acid ether compounds, pharmaceutically acceptable salts thereof, a preparation method thereof, a pharmaceutical composition and an application of the ligustrazine stilbene polyphenol aromatic acid ether compounds. The ligustrazine stilbene polyphenol aromatic acid ether compound and the pharmaceutically acceptable salt thereof have a remarkable anti-platelet aggregation effect, and can be used for preparing drugs for resisting platelet aggregation, preventing or treating cardiovascular diseases or treating or preventing thromboembolic diseases.

The invention belongs to the technical field of functional food, and particularly relates to a compound oral liquid beneficial to maintaining the healthy level of blood pressure and a preparation method thereof.The compound oral liquid is prepared from, by weight, 4-12 parts of Shanxi mature vinegar, 3-12 parts of folium cortex eucommiae and 3-12 parts of folium apocyni veneti. The traditional Chinese medicine composition contains various antihypertensive active substances such as melanoidins and ligustrazine, two traditional Chinese medicinal materials such as folium cortex eucommiae and folium apocyni veneti which have an antihypertensive effect are matched, the traditional Chinese medicinal materials are rich in antihypertensive components such as flavonoid and phenolic acid, the optimal proportion is obtained through multiple tests, and the efficacy of cooperatively promoting and maintaining the blood pressure health level is achieved. A series of complications caused by hypertension are relieved, the healthy level of blood pressure can be maintained and tends to be stable, and the three components have a remarkable synergistic interaction effect according to the prescription range and the preparation method disclosed by the patent.

The application provides a triazole compound for protecting brain tissue and a preparation method thereof, and belongs to the technical field of biological medicines, and provides a triazole compound as shown in formula (I) and chiral isomers, enantiomers, diastereoisomers, geometric isomers, free forms or pharmaceutically acceptable salts, hydrates, solvates or esters thereof; and also provides a preparation method thereof; compared with the prior art, the application combines the internal ester ring-opening product of butylphthalide and ligustrazine through triazole to synthesize a novel triazole compound which can be used for protecting brain tissue; the preparation method of the triazole compound is simple and the raw materials are easy to obtain; the triazole compound has better biological activity and brain tissue protection effect than butylphthalide / ligustrazine, and has significant protection activity on a SH-SY5Y cell damage model.

This invention discloses a method for increasing the tetramethylpyrazine content in a soy sauce-flavored baijiu (a type of starter culture). The method includes adding a plant additive composed of Eucommia ulmoides leaves, Malus spectabilis leaves, Polygonum hydropiper, Astragalus membranaceus, and Angelica dahurica to the raw materials used in preparing the baijiu. The plant additive is formulated in the following proportions by dry weight: Eucommia ulmoides leaves 2-3 parts by weight, Malus spectabilis leaves 1-2 parts by weight, Polygonum hydropiper 1-2 parts by weight, Astragalus membranaceus 1-1.5 parts by weight, and Angelica dahurica 0.5-1 parts by weight. This invention provides a simple, low-cost, and naturally safe method that effectively increases the tetramethylpyrazine content in the baijiu by adding a specific combination of plant materials, thereby improving the fermentation performance of the baijiu and the final flavor quality of the baijiu.

The invention provides a ligustrazine succinic acid eutectic crystal as well as a preparation method and application thereof, and belongs to the technical field of eutectic crystal manufacturing. The structural formula of the ligustrazine and succinic acid eutectic crystal is shown as formula I, the molecular formula of the ligustrazine and succinic acid eutectic crystal is C12H18N2O4, in the ligustrazine and succinic acid eutectic crystal, the molar ratio of ligustrazine to succinic acid is 1: 1, and the ligustrazine and succinic acid eutectic crystal has good lipid solubility, so that succinic acid in the ligustrazine and succinic acid eutectic crystal can well play a role in a deep target spot.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

The invention belongs to the technical field of medicines, and discloses a ligustrazine derivative modified based on a monosaccharide structural unit and a preparation method of the ligustrazine derivative. According to the invention, a monosaccharide structural unit is introduced to carry out glycosylation modification on ligustrazine molecules, so that the water solubility, the biological activity and the drug effect durability of ligustrazine are remarkably improved, and the pharmacological characteristics of ligustrazine are optimized; the glycosylation modification not only can improve the solubility and stability of ligustrazine, but also can improve the treatment effect of the ligustrazine in specific diseases (such as alcohol addiction and the like) by increasing the affinity of the ligustrazine to a specific target spot; through the technical scheme, the defects of the existing ligustrazine derivative in the aspects of bioavailability, drug effect persistence and the like can be overcome, and the treatment effect and clinical application value of the ligustrazine derivative are remarkably enhanced; the glycosylated and modified ligustrazine derivative has better targeting property and stability, is suitable for wider clinical treatment requirements, and particularly shows greater application potential in treatment of diseases such as alcohol dependence.

This invention, entitled "A Pharmaceutical Composition, Formulation, Preparation Method, and Application for Alleviating Cerebral Ischemia-Reperfusion Injury," belongs to the field of pharmaceutical technology. The technical problem to be solved is to improve the efficacy of drugs in preventing and / or treating cerebral ischemia-reperfusion injury. The key technical solution is a pharmaceutical composition for alleviating cerebral ischemia-reperfusion injury, comprising sinapic acid and a second therapeutic agent, wherein the second therapeutic agent comprises at least one of edaravone, citicoline, and tetramethylpyrazine.

The invention belongs to the technical field of surgical supplies, and particularly relates to a soluble microneedle and a ligustrazine transdermal patch. The soluble microneedle is creatively adopted as a physical penetration enhancing means, the transdermal patch taking the soluble microneedle and ligustrazine into account is successfully prepared, the preparation prescription of the transdermal patch is established and optimized, quality evaluation is carried out, and pharmacokinetic parameters in a rat body are determined. Through research, the medication compliance of patients is expected to be improved, the bioavailability of the medicine is expected to be improved, and a safer, more effective and more convenient administration way is provided for clinical application of ligustrazine.

The invention discloses an application of a piperlongumine / ligustrazine derivative in vascular restenosis diseases, the derivative is PL-Lig-11, and the structural formula of the derivative is shown as a formula I. The invention further discloses a preparation method of the piperlongumine / ligustrazine derivative. The derivative effectively blocks the transformation of VSMC from a contraction phenotype to a proliferation phenotype through targeted inhibition of a key signal protein RhoGDI3, and significantly inhibits the abnormal proliferation and migration ability of VSMC, thereby fundamentally inhibiting the pathological process of NIH. Compared with parent compounds of piperlongumine and ligustrazine, the PL-Lig-11 shows higher anti-proliferative activity and higher selectivity, and the expected cytotoxicity is lower than that of clinically common chemotherapy drugs such as rapamycin and paclitaxel. The medicine can be used for preventing and treating vascular stenosis diseases caused by restenosis after PCI (percutaneous coronary intervention), hypertensive vascular remodeling, atherosclerotic plaque progress and other operations or injuries, and a candidate medicine with a novel action mechanism and good safety is provided for clinic.

The invention relates to the technical field of compound detection, in particular to a detection method of tetramethylpyrazine. The detection method comprises the step of detecting the content of tetramethylpyrazine in a to-be-detected sample by using an ion mobility spectrometry technology, wherein the to-be-detected sample is white spirit or distiller's yeast leach liquor. The detection method is accurate in detection and simple to operate, harmful solvents such as acetonitrile and a chromatographic column do not need to be used, and detection equipment is simplified.

The present invention discloses a method for brewing health-care liquor. First, Chinese herbal medicines are crushed and added to the tail of the liquor for soaking. Then, sorghum is taken and prepared into a mature grain mash by moistening and steaming the grain. The tail of the Chinese herbal medicine liquor is sprinkled on the liquor. High-temperature Daqu is added to gather and accumulate the residue. The liquor is placed in a sealed pit for fermentation for 30-40 days. The first round of liquor is steamed and taken. The lees after the previous round of liquor steaming are added. The tail of the Chinese herbal medicine liquor is sprinkled on the liquor. High-temperature Daqu is added to gather and accumulate the residue. The liquor is placed in a sealed pit for fermentation for 30-40 days. The second round of liquor is steamed and taken. Repeat five rounds, and the third, fourth, fifth, sixth, and seventh rounds of liquor are taken respectively. The liquors of different rounds are subjected to large and small slurries to obtain health-care liquor. The health-care liquor prepared by the present invention is a sauce-flavored liquor. In addition to containing beneficial components of Chinese herbal medicines, it also contains more flavor substances such as tetramethylpyrazine and guaiacol, which makes the sauce-flavored liquor have a mellower taste.

The invention discloses a method for detecting ligustrazine by using a fluorescent probe, the probe is a Tb-EDTA complex, and is formed by coordination of Tb < 3 + > and ethylenediamine tetraacetic acid; based on a fluorescent quantitative analysis method, the probe is used as a recognition element, and the ligustrazine content in a liquor sample is detected. The probe is formed by coordination of Tb < 3 + > and EDTA (ethylene diamine tetraacetic acid), and a charge transfer (LMCT) process from a ligand to metal is regulated and controlled by utilizing a specific coordination effect between ligustrazine (TMP) and a Tb < 3 + > center, so that fluorescence quenching at 548 nm and new fluorescence peak enhancement at 650 nm of the probe are caused, and ratio type response is formed. The probe is simple to prepare and good in stability, and has a wide linear detection range (0.1-40 [mu] mol / L), a low detection limit (0.038 [mu] mol / L) and excellent selectivity on TMP. The probe is successfully applied to quantitative detection of TMP in complex actual samples such as soy sauce type white spirit, sake and urine, the recovery rate is high, repeatability is good, and a new powerful tool is provided for food flavor analysis and biological medicine monitoring.

The invention belongs to the technical field of natural medicines, and particularly relates to application of ligustrazine in preparation of medicines for promoting lymphatic neogenesis and in-vitro activation of a VEGFC / VEGFR3 signal channel. It is found for the first time that ligustrazine can remarkably enhance the proliferation and migration capacity of HLECs, the number of lymphatic vessels in an infarct marginal area can be increased after MI, inflammatory cell infiltration can be relieved, the content of VEGFC in serum can be increased, expression of lymphatic vessel neogenesis related protein and genes can be promoted, the heart function can be improved, and myocardial cell damage can be relieved. The invention also finds that ligustrazine can activate a VEGFC / VEGFR3 signal channel. The invention provides a new active ingredient for developing a lymphatic vessel regulator for promoting lymphatic vessel neogenesis, provides a new thought and method for cardiac tissue repair and function improvement after MI, and also provides a new modeling drug for constructing a lymphatic metastasis model by activating a VEGFC / VEGFR3 signal channel in vitro and evaluating the curative effect of the anti-lymphatic metastasis drug.

The application discloses a preparation method of adamantane nitroketone derivatives and application of the adamantane nitroketone derivatives in ischemic stroke drugs. The adamantane nitroketone derivatives are combined by 2,3,5,6-tetramethylpyrazine and adamantane compounds through nitroketone. The compound not only has strong free radical scavenging capacity, but also integrates the nerve protection function of the adamantane compound.

The application discloses a preparation method of honokiol-tetramethylpyrazine eutectic crystal, which comprises the following steps: mixing honokiol and tetramethylpyrazine, ball milling / stirring, and drying to obtain honokiol-tetramethylpyrazine eutectic crystal. The application further discloses honokiol-tetramethylpyrazine eutectic crystal prepared by the above method. The application further discloses a composition comprising the above honokiol-tetramethylpyrazine eutectic crystal and honey. The application further discloses the use of the above honokiol-tetramethylpyrazine eutectic crystal or the above composition in the preparation of drugs for treating cardiovascular and cerebrovascular diseases, resisting platelet aggregation and resisting tumors.

The invention provides a composition, a biostimulant and application of the biostimulant, and the composition comprises an acetoin aqueous solution, 2, 3-butanediol, tetramethylpyrazine and phenylalanine. According to the composition provided by the embodiment of the invention, efficient and stable targeted color change is realized through triple synergistic effects of signal transduction (acetoin and 2, 3-butanediol)-metabolic regulation (tetramethylpyrazine)-precursor supply (phenylalanine), and meanwhile, the color change effect of the composition is improved. The biostimulant containing the composition and the peptide amino acid liquid can be applied to a plurality of stages such as a post-transplanting stage, a pre-flowering stage, a fruit setting stage, a fruit swelling stage and a color changing stage, so that stable transition and synergistic promotion of crops from vegetative growth to reproductive growth are effectively realized, flower promotion, fruit retention, fruit swelling and color changing are comprehensively realized, furthermore, yield increase and advanced harvesting of the crops can be realized, and the economic benefit of the crops is increased. Meanwhile, the raw materials for improving the sweetness and the vitamin content of the fruits are easy to obtain, the preparation method is simple, industrialization is easy to achieve, and high use value is achieved.

The application discloses a preparation method of oil-tea camellia seed oil with nut aroma, and comprises the following steps: 1) pretreating oil-tea camellia seed shells and oil-tea camellia fruits to obtain a fermentation substrate; 2) preparing a fermentation medium containing the oil-tea camellia seed shells and the oil-tea camellia fruits; 3) yeast fermentation: inoculating yeast into the fermentation medium to perform fermentation, the fermentation time is 36-42 hours, and a fermentation product is obtained; 4) enzymatic fermentation: adding glucose oxidase into the fermentation product to perform enzymatic hydrolysis and deglucose treatment, the enzymatic hydrolysis and deglucose treatment is accompanied by continuous fermentation of the yeast, the enzymatic hydrolysis and deglucose treatment time is 24-30 hours, and a fermentation enzymolysis liquid is obtained; and 5) cold pressing: drying and steaming the fermentation enzymolysis liquid and then performing cold pressing to obtain the oil-tea camellia seed oil. The method of first adopting yeast fermentation and then adopting glucose oxidase enzymolysis makes the content of tetramethylpyrazine in the prepared oil-tea camellia seed oil reach 2120 ug / mL, gives the oil-tea camellia seed oil very strong nut aroma, and improves the flavor quality of the oil-tea camellia seed oil.