The present invention discloses a selenium-rich dietary fiber biscuit for preventing diabetes and a preparation method of the selenium-rich dietary fiber biscuit, and belongs to the technical field offood processing. The selenium-enriched dietary fiber biscuit is prepared by using apple pomace powder, oat flour, buckwheat flour, red bean flour and wheat flour as main raw materials, using mushroompowder, low-fat milk, eggs, olive oil, pumpkins, bitter gourds, celery, Ziyang selenium-enriched green tea and other foods as auxiliary materials, and also being supplemented with parts of vitamins,trace elements, a traditional Chinese medicine extract mainly containing medicinally and edibly homogenous substances, xylitol, a swelling agent, edible salt, etc. The selenium-enriched dietary fiberbiscuit is prepared by raw and auxiliary material preparing, mixing, dough preparing, baking and packaging. In the raw and auxiliary materials of the biscuit, the six substances of high dietary fiberapple pomace powder, food for reducing blood sugar, blood fat and blood pressure, high protein food, vitamins, trace elements and formula traditional Chinese medicines are selected, so that the selenium-rich dietary fiber biscuit plays a role in integrally regulating metabolism of a plurality of systems and various substances of human body, further reduces risk of diabetes, plays an effective regulation function on glucose metabolism of organism from multi-targets, and at the same time also plays functions of preventing diabetes, assisting in reducing glucose and assisting in preventing type 2diabetes complications. Aiming at 4 common constitutions of yin deficiency type, qi-yin deficiency type, yang deficiency type and mild type, 4 biscuits with different formulas are prepared, so that health-care effects of the biscuit is more accurate and effective. The prepared biscuit is rich in nutrition, can also effectively improve dietary nutritional structure of people, and is beneficial toprolonging life after being eaten for a long time.