11results about How to "Low specificity" patented technology

The invention belongs to the technical field of stem cell development and differentiation, and discloses application of polypeptide DGEA or a derivative thereof in promoting pluripotent stem cells to be differentiated into mesenchymal stem cells. The invention specifically discloses application of the polypeptide and the derivative thereof in promoting differentiation of pluripotent stem cells into pluripotent stem cells or preparing products for promoting differentiation of pluripotent stem cells into pluripotent stem cells. The amino acid sequence of the polypeptide is DGEA. The invention discloses the application of DGEA in promoting differentiation of pluripotent stem cells (embryonic stem cells, embryonic germ cells or induced pluripotent stem cells) into pluripotent stem cells (mesenchymal stem cells) for the first time, and the effect of DGEA is verified through corresponding experiments. The DGEA can obviously improve the efficiency of differentiating the embryonic stem cells, the embryonic germ cells or the induced pluripotent stem cells into the mesenchymal stem cells, and the obtained mesenchymal stem cells are high in safety and high in differentiation capacity.

The invention discloses application of a Cpeb3 gene inhibitor and a copper death inhibitor in preparation of a medicine for treating myocardial ischemia-reperfusion injury, and relates to the technical field of myocardial ischemia-reperfusion injury. By specifically reducing or knocking out Cpeb3 expression, myocardial repair can be remarkably promoted, action potential stability is improved, and the death rate is reduced. Therefore, Cpeb3 is a specific intervention target spot of copper death in Gpc6 + CMs. The small molecule inhibitor of Cpeb3 can remarkably reverse changes of copper death, aerobic respiration and / or glycolysis caused by Cpeb3 activation. In addition, the copper death inhibitor can improve the survival rate of myocardial cells, reverse the change of aerobic respiration and / or glycolysis induced by Cu, and reverse the change of glycolysis, pentose phosphate pathway and tricarboxylic acid cycle metabolites caused by Cu stimulation. Therefore, the invention provides a new solution for prevention and treatment of myocardial ischemia reperfusion injury, and has a good application prospect.

The invention relates to the technical field of biology, in particular to a polypeptide combination and application thereof. The invention provides a human giant cell IgM epitope polypeptide. The invention also provides a method for coupling the epitope polypeptide with horse radish peroxidase. The invention also provides a detection method of the epitope polypeptide coupled horseradish peroxidase applied to the human giant cell IgM detection kit. The method has the advantages that the two epitope polypeptides are identified for the first time to detect positive serum samples of human cytomegalovirus IgM, the human cytomegalovirus IgM detection kit adopting the polypeptides as specific antigens has excellent sensitivity and specificity, and the standard is easy to control.

The present invention relates to agents, compositions, and methods for treating or ameliorating symptoms of cancer. Exemplary synergistic therapies include the use of antisense oligonucleotide agents to suppress expression of TGF-β2, alone and in combination with interferon-gamma. One or more biomarkers can be used to select subjects for treatment.

The invention relates to a MuSK antibody detection method and a corresponding product. The polynucleotide adopted by the invention is the polynucleotide for coding the recombinant human Flag-MuSK-ECD protein, and the recombinant human Flag-MuSK-ECD protein has the advantages that the recombinant human Flag- The sequence of the recombinant human Flag-MuSK-ECD protein is as shown in SEQ ID NO. 2; the sequence of the polynucleotide is as shown in SEQ ID NO. 1. According to the MuSK antibody detection method, eukaryotic cells are transfected through artificially constructed target gene expression plasmids, secreting type Flag-MuSK-ECD protein in supernatant is collected, the secreting type Flag-MuSK-ECD protein is used as a detection antigen, and the MuSK antibody is detected through the detection antigen. Factors which influence antigen activity detection due to reasons such as detection of antigen species difference, expression of immature MuSK full-length protein by prokaryotic cells, low protein purification purity caused by partial anti-MuSK transmembrane segment and / or intracellular segment antibodies and a poor-specificity protein tag purification system are avoided, so that the antigen activity is relatively good; therefore, the specificity and the sensitivity of pathogenic antibody detection are improved, and a more valuable biomarker detection method is provided for clinical diagnosis and treatment schemes.

This invention discloses a method for delivering a VIGS vector targeting the flower buds inside the bulb of Lycoris radiata, a method for verifying gene function, and their applications, belonging to the field of biotechnology. The method includes: making 4-6 holes around the root plate of a dormant Lycoris radiata bulb, and 2-4 holes in the central area of ​​the bulb base, reaching the meristematic region of the flower bud; immersing the treated bulb in VIGS infection solution up to the wounds on the root plate; and performing vacuum treatment to ensure effective penetration of the infection solution into the internal flower bud tissue. This invention successfully delivered a silencing vector targeting the LrP5CS1 gene to the flower bud, specifically reducing its expression and obtaining a stable early-flowering phenotype in Lycoris radiata, thus verifying the gene function. Therefore, this invention establishes an efficient delivery platform that can be used for rapid screening and verification of other key genes regulating the flowering period and ornamental traits of Lycoris radiata, providing a novel technical means for the functional study of genes related to the flowering phenotype of Lycoris radiata.

The present invention discloses a non-antibody-dependent protein ubiquitination enrichment method, including whole protein extraction, complete blocking of free amino groups at the protein level, hydrolysis of ubiquitin chains on substrate proteins, labeling of free amino groups at ubiquitination sites with enrichable label reagents, protein enzymatic cleavage, and enrichment of labeled ubiquitinated peptides. The present invention also relates to a detection method, i.e., liquid phase-mass spectrometry detection of the enriched ubiquitinated peptides. The present invention does not require the use of antibodies to recognize the ubiquitin residual motifs produced after enzymatic cleavage, and can achieve indiscriminate enrichment and identification of protein ubiquitination.

The invention aims to provide a multiple fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method for detecting three pigeon diarrhea viruses, namely a fluorescent quantitative RT-PCR detection method capable of simultaneously detecting a pigeon Newcastle disease virus, a pigeon rotavirus and a pigeon adenovirus in one reaction so as to realize safe, specific, sensitive, rapid and convenient detection on pigeon diarrhea virus diseases. Through screening of the primer pair and the probe group, three common pigeon diarrhea diseases, namely the pigeon Newcastle disease virus, the pigeon rotavirus and the pigeon adenovirus, can be safely, specifically, quickly, sensitively, simply and quickly detected with high flux, so that the defects of the existing traditional detection technology are overcome.

The invention relates to the technical field of biosensing, in particular to a light addressing high-flux photoelectrochemical biosensor and a preparation method and application thereof, the sensor comprises a substrate, and a working electrode, a reference electrode, a counter electrode and a mobile light source which are arranged on the substrate; the substrate is provided with a plurality of detection areas which are distributed in an array, and the working electrodes comprise a plurality of array working electrodes which are distributed in different detection areas; the array working electrode is sequentially modified with a photoactive material, a DNA (Deoxyribose Nucleic Acid) concatemer sensing interface and circulating tumor cells; wherein the light active material is an alpha-Fe2O3 / Bi2S3 II type heterojunction, and the light active material is a Bi2S3 II type heterojunction. The reference electrode is a saturated Ag / AgCl electrode, the counter electrode is a platinum electrode, and the moving light source is an infrared laser pen. According to the light-addressing high-throughput photoelectrochemical biosensor provided by the invention, the defects that high-throughput CTC detection cannot be realized in the existing detection technology and the like are overcome by constructing a multi-detection-region detection array photoelectrochemical biosensing electrode.

The invention discloses a PJI diagnosis model based on serum and synovial fluid indexes and a construction method of the PJI diagnosis model, and aims to solve the problems that in existing PJI diagnosis, specificity of a single index is insufficient, and misjudgment is easily caused due to culture negative. According to the method, serum and joint fluid samples and clinical information of all-joint replacement postoperative revision patients are collected, multiple types of clinical indexes are extracted after data processing and queue division, and eight core indexes are screened out by adopting LASSO logistic regression in combination with single-factor and multi-factor logistic regression; and constructing a visual column graph model containing a scoring system and a probability transformation formula through an R language. The model fully considers index weight and interaction, has the characteristics of low cost, easy acquisition and accurate diagnosis, is reliable in performance through training and verification queue verification, can assist clinicians in making scientific judgment before invasive treatment and reduce unnecessary detection and medical burden, and is suitable for popularization and application of medical institutions at all levels.

The present invention provides a multiplex PCR primer and probe combination for detecting 9 types of human papillomavirus DNA, its application, and a kit using the combination. The present invention provides a primer and probe combination for detecting the above 9 types of HPV, namely HPV16, HPV 18, HPV 58, HPV 52, HPV 6, HPV 45, HPV 11, HPV 33 and HPV 31, targeted by HPV vaccines, and provides a corresponding nucleic acid detection kit on this basis. The kit of the present invention is based on the POCT detection method, and one sample is subjected to three reactions to detect 9 types of HPV. The reagent has the advantages of being able to be transported, stored and operated at room temperature, being convenient and fast, having low cost, having good detection specificity and high sensitivity. At the same time, the kit can be used to monitor the dynamic changes of infection after HPV vaccination, and long-term follow-up after vaccination can provide more accurate guidance for vaccination strategies, provide a scientific basis for the formulation and adjustment of vaccination plans and vaccine research and development, and facilitate the clinical timely adoption of accurate and effective detection and treatment methods. In addition, the present invention uses endogenous internal references, that is, by using internal reference β-globin, to effectively control sample collection, nucleic acid extraction and sample extraction-free direct amplification.