256results about How to "Specific detection" patented technology

The present invention relates to an automatable method for obtaining an improved diagnosis of cancer, and its precancerous stages in uterine cervical smears by simultaneously staining and detecting at least two different molecular markers, which exhibit a disease-associated change in gene expression, in a cell by means of using antibodies or nucleic acid probes.

The invention provides preparation and application of a quantitative and rapid detection biosensor of microcystic toxin-LR, and belongs to the biotechnology field. The detection sensor is an impedance type electrochemical sensor, and consists of a CHI760C type electrochemical work station and a three-electrode system in which an immunity electrode is taken as a working electrode, a saturated calomel electrode is taken as a reference electrode and a platinum wire electrode is taken as a counter electrode; L-cysteine, colloidal gold and microcystic toxin antibody compound are respectively enveloped on a surface of a bare mitallic electrode to be taken as the working electrode; a change of electric potential difference between the reference electrode and the working electrode takes place when the microcystic toxin antibody is combined with the antigen (MC-LR) in sample solution, and is subject to a quantitative relation with the concentration of the antigen in the sample, then the detection of the microcystic toxin is carried out; the biosensor for rapidly, specifically and sensitively detecting microcystic toxin antigens is successfully established; the detection linear range is 0.05-300ng / mL, and the detection limit is 1.82*10<-2>ng / mL. The biosensor can perform real-time monitoring on natural lake water, and can also perform real-time monitoring on preparation process of drinking water in water supply works.

The present invention relates to new methods for the specific detection, quantification and / or differential diagnosis of neurodegeneration in an individual making use of a combination assay detecting at least three neurological markers in one or more body fluids of said individual, the type and degree of neurodegeneration being reflected in the quantitative changes in the level of all of said neurological markers compared to the control sample. The present invention also relates to methods for the detection of Rab3a, SNAP25 and alpha-synuclein in cerebrospinal fluid and to the use of these methods in a combination assay for specific detection, quantification and / or differential diagnosis of neurodegeneration.

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I / D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

The invention discloses an FAdV-4 PCR detection kit and detection method. The kit comprises protease K, a lysing solution, sodium acetate, saturated phenol, chloroform, isoamylol, anhydrous ethanol and sterile distilled water, and is characterized by comprising 2*TaqPCR Mix, a sense primer and a reverse primer, wherein the sequence of the sense primer is shown as SEQ ID No.1, and the sequence of the reverse primer is shown as SEQ ID No.2. The kit can be used for detecting FAdV-4. The method for detecting FAdV-4 by adopting the kit is also provided, and FAdV-4 can be detected quickly and specifically.

There is presently provided a device for detecting an analyte particle in a sample. The device comprises a chamber having an interior surface upon which is located an electrode array. The electrode array comprises pairs of electrodes, each pair having an inner electrode and an outer electrode that substantially surrounds the inner electrode. Each pair of electrodes is coated with a capture molecule that recognises and binds the analyte particle that is to be identified and quantified. The device uses a combination of dielectrophoresis and impedance measurements to capture and measure analyte particles from a sample.

The invention provides a fungaltoxin duplex detection method based on Raman beacon molecular coding silver @ gold core-shell nanometer particles, and belongs to the field of material chemical application. The invention has the main content of providing a simple and controllable preparation method of the Raman beacon molecular coding silver @ gold core-shell nanometer particles. A novel multiplex Raman sensing detector is built; the simultaneous fast specificity detection of two kinds or even various kinds of fungaltoxin is realized. The Raman beacon molecules are modified on the surfaces of silver nanometer particles; then, the growth of a layer of gold shell is performed; different Raman beacon molecular coding silver @ gold core-shell nanometer particles are prepared through regulating and controlling the pH of a buffer system and the types of the Raman beacon molecules; the Raman beacon molecule Raman signal intensity change and the generating mechanism under the electromagnetic field coupling effect between silver cores and gold shells are studied; the fungaltoxin aptamer specificity recognition principle and the good magnetic response of magnetic nanometer particles are combined; the multiplex Raman sensing detector is built; the simultaneous fast and specific detection of the aflatoxin and the ochratoxin on the basis of the Raman signals is realized for the first time.

The invention discloses a method for detecting HBVDNA through combination of PCR and CRISPR. The method comprises the steps of (1) amplifying nucleic acid of samples to be detected through a pair of upstream and downstream specific primers, wherein a sequence which can be recognized and transcribed by T7RNA polymerase is arranged at a 5' end of the downstream primer; and (2) detecting whether a targeting sequence exists or not in amplification products of the nucleic acid of the samples to be detected in a detection system containing crRNA, T7RNA polymerase, Cas13a protein and report RNA for recognizing an HBVDNA target sequence, wherein the section of the HBVDNA target sequence is 803<rd>-829nt. The invention further discloses crRNA capable of targeting HBVDNA specific sites and a reagent kit containing the crRNA. The method provided by the invention is simple, convenient and quick during detection of the content of the HBVDNA, and has extreme high sensitivity and specificity, single-copy HBV DNA is differentiated within 15 minutes after PCR amplification, and higher positive detection rate can be shown for serum samples low in virus load.

The invention relates to a fluorescence bio-sensing method for detecting glutathione. According to the invention, a manganese dioxide nanometer material is used as an oxidizing agent and a recognition element; in the absence of glutathione (GSH), an oxidized o-diaminobenzene product is generated by catalytically oxidizing the manganese dioxide nanometer material for fluorescence detection; in the presence of the GSH, the manganese dioxide nanometer material is degraded by the GSH, the oxidizing capacity of manganese dioxide to o-diaminobenzene is inhibited and the fluorescence signal is weakened. The GSH detection is realized in the manner of comparing fluorescence values. Compared with the prior art, the method provided by the invention has the advantages of simple and convenient operation, high sensitivity, simplicity, low cost and specific detection for GSH. The detection method provided by the invention can be used for effectively detecting the GSH in a biological liquid sample.

There is provided a monitoring system which includes a plurality of monitoring cameras and a management device. The management device transmits feature information relevant to a person to one or more first monitoring cameras and receives check results, and transmits time information in which the person is captured to second monitoring cameras based on the check results. The management device specifies the person based on a check result acquired in such a way that the second monitoring cameras perform a check using the time information.

The invention relates to a gene analysis detecting product, in particular to a gene detecting chip and a reagent matched with the same, more particular to a gene detecting chip and a matched reagent used for detecting a series of gene mutations that are closely related to the reactiveness of medicines for treating malignancy as well as the application thereof. The gene chip can detect known gene mutations closely related to individual difference of anti-tumor medicine reaction in comprehensive, systemic and high-flux ways by selecting appropriate mutant sites and designing appropriate primers and probes and can work out corresponding adjusting schemes aiming at different medicines.

The invention discloses an electrochemical sensor for adapters and a method for detecting chlorpyrifos. The electrochemical sensor adopts a three-electrode system and comprises a first detection layer, a second detection layer and a fixed layer. A preparation method comprises the following steps of: preparing copper oxide nanoflower solution, preparing single-wall carbon nanotube solution, treating base electrodes, activating carboxyl groups, and fixing probes and the adapters; and then manufacturing a standard curve by utilizing a differential pulse voltammetry, and finally calculating out the concentration of the chlorpyrifos in samples according to the standard curve of the chlorpyrifos. The electrochemical sensor and the method disclosed by the invention have the advantages that the detection for the chlorpyrifos is specific, the sensitivity and the selectivity are high, the renewability is achieved, the materials are wide in source, the cost is low, and the preparation method is simple, so that the application prospect is good.

The invention relates to a gene detection chip, in particular to a gene detection chip used for detecting common major gene mutation of organic anion transferred polypeptide 1B1 (OATP1B1 in short), which is closely related to reactiveness of medicines used for curing high blood pressure, malignant tumor, 2 type diabetes mellitus, hypercholesterolemia. Through selecting 8 mutational sites and designing out corresponding probes, the invention realizes the detection on the mutational sites and is capable of providing information of the mutation sites, thereby providing foundation for medication guiding.

The invention belongs to the gene field of biotechnology, and relates to a single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and an application thereof. The invention provides a gene partial sequence (the sequence is shown by SEQ ID No.1) of the genetic area 6p21.3 and an SNP site rs9275319 (the sequence is shown by SEQ ID No.2). The invention also provides a method for detecting the SNP site, a specific nucleic acid primer with sequences shown by SEQ ID No.3 and SEQ ID No.4, and a UEP primer with a sequence shown by SEQ ID No.5. The invention also relates to a detection kit for the SNP site rs9275319 and an application thereof. In the invention, genotyping can be performed on the rs9275319 site, the method is simple and quick, the result is accurate and clear, and a new means is provided for the liver cancer susceptibility identification as well as prevention and treatment.

The invention provides an LAMP primer for detecting Brucella and a kit containing the same. The primer comprises 2 inner primers ( FIP, BIP ), 2 outer primers ( F3, B3 ) and 2 loop primers ( LB, LF ) (shown as Seq ID No.1-6). According to the invention, the LAMP primer isothermal amplification technology is applied to the rapid detection of Brucella, so as to fast, accurately and conveniently detect the Brucella in a serum specimen. The method has higher specificity and sensitivity than a conventional PCR method, and has vital significance to early prevention and pathogenic monitoring of Brucella; at the same time, the invention can avoid high equipment investment, and is more suitable for Brucella epidemic survey site or local health and epidemic prevention.

The invention relates to a detection chip for familiar resistant organisms and its preparation and application methods belonging to microorganism detection field. The invention employs DAN chip method including fixing the synthesized oligonucleotide probes for clinical detection of familiar resistant organisms on the slide surface to form a dot-plot, hybridizing the pending sample DNA with the chip to obtain a great deal of gene sequence information relative to the bacteria identification and drug tolerance, identifying the kinds of the familiar pathogenicbacterias existing in the clinical sample, thus to implement the identification of the clinical familiar pathogenicbacterias and detection of the main drug resistant spectrum. The invention is simple, convenient, quick, sensitive and specific, and can obviously shorten the disease diagnosis time.

The invention discloses preparation of a bionic molecular imprinting electrochemical sensor and based on click chemistry and a detection method of food allergen. The method includes selecting nitrine alkyl sulfhydryl fixed with the surface of an electrode to prepare a self-assembly single layer film of a nitrine end group, utilizing click chemistry reaction to conduct end group alkenyl functionalization on the self-assembly single layer film on the nitrine end group, selecting a function single body capable of reacting with the allergen to combine molecularly imprinted polymers (MIPs) of an allergen artificial antibody, utilizing the existing method to prepare a graphene material, mixing the graphene material to prepare the artificial antibody, evenly mixing template molecules of the food allergen, the function single body, the graphene, crosslinked agent, pore-foaming agent, initiator and organic solvent according to certain substance amount to prepare the bionic MIPs mingled with the graphene, connecting a prepared MIP modification work electrode to an electrochemical working station and detecting the food allergen in food extraction liquid.

The invention discloses a method for specifically detecting pentachlorophenol based on CdS quantum dots. The pentachlorophenol is an electron-rich body, and can be absorbed on the surface of CdS<*+> and is oxidized into tetrachlorobenzoquinone by hole h<+>, CdS<*+> quantum dots are consumed, and electroluminescent signals of the quantum dots are weakened, so that the pentachlorophenol is detected by detecting the change of the electroluminescent signals of the quantum dots. Compared with the traditional method, the invention has the advantages that: the method is high in sensitivity and specificity, and pentachlorophenol at the concentration of 0.008nmol / L can be detected by the method; meanwhile, the detection time is short, the treatment of a sample is simple, and the whole detection process spends not more than 30min; and the novel method can simply, conveniently, quickly and specifically detect the pentachlorophenol.

The invention discloses a reagent kit for the chemiluminescence enzyme-linked immunoassay of furazolidone, which comprises a kit body, an enzyme label plate arranged in the kit body and a reagent arranged in the kit body, wherein, holes of the enzyme label plate are coated by an envelope antigen which is manufactured through coupling between 3-azyl-2-oxazolone that is a metabolin of furazolidone and ovalbumin; and the reagent contains a polyclonal antibody of furazolidone, an enzyme label secondary antibody that is a goat anti-rabbit antibody marked by horseradish peroxidase, a furazolidone-series standard solution, a concentrated phosphate buffer solution, a concentrated cleaning solution and a luminescent liquid. The reagent kit has the characteristics of high sensitivity, good reproducibility, simplicity, convenience, speediness and accuracy; compared with the traditional color comparison ELISA method, the sensitivity can be improved by one order higher, and the reagent is expected to play an important role in furazolidone residue detection of water used in stock raising, feeds and animal-derived foods (such as milk samples, animal tissue samples and urine samples).

The invention provides a fluorescence chemical sensor and a preparation method and application thereof. The structural formula of the fluorescence chemical sensor is shown as the formula (I), wherein R1 refers to sulfur or oxygen; and R2 refers to sulphonate, iodate, fluorphosphate or fluoborate. The preparation method for the fluorescence chemical sensor comprises the following steps of: (1) performing oxidation reaction on 4'-(N,N-dimethyl pyridyl amido)benzene under the action of an oxidant to obtain 4'-(N,N-dimethyl pyridyl amido)benzaldehyde; (2) reacting the 4'-(N,N-dimethyl pyridyl amido)benzaldehyde with a compound in a formula (III) to obtain a compound in a formula (II); and (3) reacting the compound in the formula (II) with copper ion-containing inorganic salt to obtain the fluorescence chemical sensor, wherein in the formulas (II) and (III), R1 refers to sulfur or oxygen; and R2 refers to sulphonate, iodate, fluorphosphate or fluoborate. The fluorescence chemical sensor can specifically, efficiently, timely and easily detect cyanide ions in aqueous solution.

The invention discloses a chemical luminescence enzyme-linked immunoassay kit of gatifloxacin, comprising a kit body, an enzyme-marked plate and a reagent which are arranged in the kit body; wherein, all the holes of the enzyme-marked plate are coated with coating antigen; the coating antigen is prepared by the coupling of the gatifloxacin and ovalbumin; the reagent comprises a polyclonal antibody of the gatifloxacin, an enzyme-marked second antibody (namely, the antibody of horseradish peroxidase-marked goat anti-rabbit), a gatifloxacin series standard solution, a concentrated phosphate buffer, a concentrative cleaning solution and a luminescence solution. The kit has the advantages of high sensitiveness, good repeatability, simpleness, fastness and exactness; and compared with traditional colorimetric ELISA method, the sensitiveness can be improved by one magnitude and the method is expected to play an import role in the gatifloxacin residual detection of animal source foods (such as milk samples and animal tissue samples) and a urine sample.

The invention discloses a porcine epidemic diarrhea virus TaqMan fluorescent quantitative RT-PCR kit and a detection method thereof. According to an N gene sequence of a porcine epidemic diarrhea virus, a specific primer and a TanMan probe are designed and synthesized; and by optimizing a fluorescent quantitative reaction condition, a TaqMan real-time fluorescent quantitative RT-PCR method for detecting PEDV (Porcine Epidemic Diarrhea Virus) is established, has good linear relationship in a range from 10<8> copies / microlitres to 10<2> copies / microlitres, has the sensitivity capable of detecting 10<2> copies / microlitres to the minimum, is high in sensibility, can specifically and rapidly detect the porcine epidemic diarrhea virus and is good in repeatability with variation coefficient of intra-batch or inter-batch smaller than 1 percent. By applying the method to detect 185 pig manure samples collected in 2015-2017, and the positive detection rate is 38.4 percent; and when a conventional RT-PCR method for detection and a controlled trial is realized, the positive detection rate is 26.3 percent.

The invention discloses universal primers and a probe for on-site rapid detection of Brucella and a kit. The forward primer sequence is shown as SEQIDNo.1, the reverse primer sequence is shown as SEQIDNo.2, and the probe sequence is shown as SEQIDNo.3. The universal primers, the probe and the kit provided by the invention for detection of Brucella have high sensitivity and strong specificity, minimumly can detect 6.0*10<0>cfu Brucella, and have no cross reaction with Escherichia coli O157:H7, yersinia enterocolitica O:9, Salmonella, Staphylococcus aureus and other bacteria. The universal primers, the probe and the kit provided by the invention not only can be used for detection of Brucella strains, but also can be used for detection of clinical samples, which mainly include blood, milk samples, aerosol samples, and tissue samples, etc. The kit provided by the invention is convenient to use, has no need for special equipment, can carry out sensitive, specific and rapid detection of Brucella on the crude split product of a to-be-detected sample in 25min just at 38DEG C, and is suitable for field or grassroots brucellosis quarantine work.

The invention discloses a specific detection method of pentachlorophenol. Pentachlorophenol is an electron-rich body, can be adsorbed to the surface of C<*+>, is oxidized into tetrachloroquinone by cavity h<+>, consumes C<*+> quantum dots, and causes weakening of a quantum dot electroluminescence signal, thus detection of pentachlorophenol is realized by detecting the change of the quantum dot electroluminescence signal. Compared with the traditional method, the method has higher sensitivity and specificity; by adopting the method, as low as 0.001nmol / L of pentachlorophenol can be detected; at the same time, the detection time is short, the sample treatment is simple, and the whole detection process does not exceed 30min; and the method is a new method for simply, conveniently, rapidly and specifically detecting pentachlorophenol.

The invention relates to the technical field of biologics, and in particular to a porcine circovirus type-3 PCR (Polymerase Chain Reaction) detection kit and a detection method. The porcine circovirus type-3 PCR detection kit contains a DNA (Deoxyribonucleic Acid) extraction reagent, 2*TaqPCR Mix, an upstream primer and a downstream primer; the sequence of the upstream primer is as shown in SEQ ID No.1; and the sequence of the downstream primer is as shown in SEQ ID No.2. By adopting the PCR detection method of the kit, the porcine circovirus type-3 can be rapidly and specifically detected.

The invention discloses an antigenic polypeptide pool capable of detecting mycobacterium tuberculosis infection. The antigenic polypeptide pool specifically stimulates mycobacterium tuberculosis infected fresh whole blood to specifically secrete IFN-gama and increase the sensitivity. The invention provides a new detection reagent for detecting mycobacterium tuberculosis infection, experiments prove that peripheral blood can be directly utilized to conduct antigenic simulation without separation of peripheral blood mononuclear cell, the experimental data shows that the antigenic polypeptide pool has high sensitivity and specificity for detecting mycobacterium tuberculosis infection, and is simple and convenient to operate and low in cost, thus having high clinical application values.

The invention relates to a method and equipment for detecting tumor microsomes, and especially relates to and discloses a method and a detector for detecting tumor microsomes by using laser tweezers and micro fluidics. The detection method comprises the steps that: laser tweezers of different polarizations are generated; dual-beam double laser tweezers are used for focusing, and particles combined with tumor microsome antibodies in a channel of a micro fluidic chip are captured by the tweezers; a sample to be detected is mixed with the particles combined with tumour microsome antibodies; changes of physical parameters of the particles combined with tumor microsome antibodies before and after being mixed with the sample are collected by the detector, and qualification and accurate quantitation of the sample requiring detection can be carried out according to the parameters. The detector comprises a laser system, a nanometer positioning system, a camera system, optical parts, mechanical parts, a data collecting and transmitting system, and automatic controlling and image processing application software. According to the invention, single tumor microsome particle can be detected, and the method and the equipment are characterized in high specificity, simple operation, short detection period, high sensitivity, and good repeatability. Also, with the method and the equipment, blood samples can be re-detected.

The invention relates to the biological technical field and discloses a detection primer of plant pathogenic fungi and a detection method thereof. The invention adopts a special primer RF4 and optimizes action conditions to invent a PCR detection method for banana fusarium wilt physiological races (Race No. 4). The method can especially, sensitively and effectively detect banana fusarium wilt physiological races (Race No. 4) and mycelium even weighting 0.1 Mug can be detected and pathogen can even be detected from the root and the stem of bananas by adopting the method.

The invention discloses a primer combination, a probe combination, an application of the primer combination or the probe combination to detection of porcine viruses, a detection reagent, a kit and a detection method. According to the primer combination, the probe combination, the application of the probe combination to detection of porcine viruses, the detection reagent, the kit and the detectionmethod, a five-porcine-virus joint detection method is established, five major porcine disease single tubes of ASFV, FMDV, CSFV, HP-PRRSV and PRV wild viruses are rapidly detected, high-throughput screening of the five viruses can be realized, the detection efficiency can be improved, and the reagent consumption can be reduced. Therefore, the cost is saved, the detection time is shortened and theworkload is reduced. The invention provides the detection reagent capable of realizing joint detection of the five porcine viruses, and the optimized primer combination and the optimized probe combination are adopted, so that the reagent and the kit adopting the reagent can specifically and sensitively detect the five porcine viruses, namely the ASFV, FMDV, CSFV, HP-PRRSV and PRV wild viruses; andfive porcine virus infections of different types of nucleic acids can be simply, quickly and accurately detected in parallel in a single reaction tube, and DNA viruses and RNA viruses can be simultaneously detected by single-tube PCR.

The invention discloses an antigen polypeptide pool for detecting Mycobacterium tuberculosis infection. The specific antigen polypeptide pool can specifically stimulate Mycobacterium-tuberculosis-infected fresh whole blood and specifically secrete IFN-gamma, thereby increasing the detection sensitivity. The invention provides a novel detection reagent applicable to detection of Mycobacterium tuberculosis infection. The experiment proves that the peripheral blood can be directly utilized to perform antigenic stimulation without the need for separating peripheral blood mononuclear cells. The experimental data shows that when being used for detecting Mycobacterium tuberculosis infection, the antigen polypeptide pool has the advantages of higher sensitivity, higher specificity and lower cost, is simple to operate, and has higher clinical application value.