The invention discloses a primer combination, a probe combination, an application of the primer combination or the probe combination to detection of porcine viruses, a detection reagent, a kit and a detection method. According to the primer combination, the probe combination, the application of the probe combination to detection of porcine viruses, the detection reagent, the kit and the detectionmethod, a five-porcine-virus joint detection method is established, five major porcine disease single tubes of ASFV, FMDV, CSFV, HP-PRRSV and PRV wild viruses are rapidly detected, high-throughput screening of the five viruses can be realized, the detection efficiency can be improved, and the reagent consumption can be reduced. Therefore, the cost is saved, the detection time is shortened and theworkload is reduced. The invention provides the detection reagent capable of realizing joint detection of the five porcine viruses, and the optimized primer combination and the optimized probe combination are adopted, so that the reagent and the kit adopting the reagent can specifically and sensitively detect the five porcine viruses, namely the ASFV, FMDV, CSFV, HP-PRRSV and PRV wild viruses; andfive porcine virus infections of different types of nucleic acids can be simply, quickly and accurately detected in parallel in a single reaction tube, and DNA viruses and RNA viruses can be simultaneously detected by single-tube PCR.