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Gene chip used for detecting related gene mutations of malignancy individual medications and application thereof

A technology for gene chips and malignant tumors, applied in the field of gene analysis and detection products, can solve problems such as disclosure of gene chip technical solutions, detection of gene mutation sites related to non-anti-tumor drug response, etc.

Inactive Publication Date: 2010-01-06
湖南宏灏基因生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no gene chip and its technical solution for detecting the above-mentioned anti-tumor drug response-related gene mutation sites

Method used

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  • Gene chip used for detecting related gene mutations of malignancy individual medications and application thereof
  • Gene chip used for detecting related gene mutations of malignancy individual medications and application thereof
  • Gene chip used for detecting related gene mutations of malignancy individual medications and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Preparation of a chip detection system for gene mutations related to individualized medicine for malignant tumors

[0060] 1. The main raw and auxiliary materials and equipment used for preparation (as shown in Table 5)

[0061] table 5

[0062] Reagents and equipment

place of origin

Reagent purity / concentration

Blank Ultraflat

Shanghai Baiao Technology Co., Ltd.

/

Aminosilane reagent

Acros (United States)

99wt.%

glutaraldehyde

Acros (United States)

25wt.%

95% ethanol

Shanghai Chemical Plant

Analytical pure

Glacial acetic acid

Shanghai Chemical Plant

Analytical pure

NaBH 4

Shanghai Chemical Plant

Analytical pure

Na 2 HPO 4

Shanghai Chemical Plant

Analytical pure

K H 2 PO 4

Shanghai Chemical Plant

Analytical pure

Na 2 CO 3

Shanghai Chemical Plant

Analytical pure

NaHCO 3

Shanghai Che...

Embodiment 3

[0122] [Example 3] Comparison of hybridization effects of probes with different base numbers

[0123]In the present invention, when the probe of the sequence shown in SEQ ID NO.1-48 is actually used, a continuous 14-25 base sequence can be selected to synthesize the probe. The following experiments are designed to prove the difference in the number of bases and The sensitivity and specificity of the results are not affected.

[0124] The following mutant and normal probes of different lengths were synthesized for GSTP1*B:

[0125] Wild-type probe I (13th to 26th base sequence of SEQ ID NO.1, 14 bases in total)

[0126] 5'-gggaga gtatttg-3'

[0127] Mutant probe I (the 13th to 26th base sequence of SEQ ID NO.3, a total of 14 bases)

[0128] 5'-gggaga gtatttg-3'

[0129] Wild-type probe II (12th to 30th base sequence of SEQ ID NO.1, 19 bases in total)

[0130] 5'-tgagggaga gtatttgca-3'

[0131] Mutant probe II (12th to 30th base sequence of SEQ ID NO.3, 19 bases in t...

Embodiment 4

[0139] The above description of the present invention does not limit the present invention, and those skilled in the art can make various changes and adjustments according to the present invention, as long as they do not depart from the spirit of the present invention, all should belong to the scope of the appended claims of the present invention. [Example 4] Negative quality control probe and positive quality control probe design scheme

[0140] In the present invention, for the actual use of the probe of the sequence shown in SEQ ID NO.49-71, continuous 14-25 base sequences can be selected to synthesize negative quality control probes at each site, and SEQ ID NO. 81 were used as positive quality control probes. The following experiments are designed to prove that the selection of the above sequences as negative quality control probes and positive quality control probes can indeed play a role in controlling the hybridization process.

[0141] Design wild, mutant and negative...

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Abstract

The invention relates to a gene analysis detecting product, in particular to a gene detecting chip and a reagent matched with the same, more particular to a gene detecting chip and a matched reagent used for detecting a series of gene mutations that are closely related to the reactiveness of medicines for treating malignancy as well as the application thereof. The gene chip can detect known gene mutations closely related to individual difference of anti-tumor medicine reaction in comprehensive, systemic and high-flux ways by selecting appropriate mutant sites and designing appropriate primers and probes and can work out corresponding adjusting schemes aiming at different medicines.

Description

technical field [0001] The invention relates to gene analysis and detection products, in particular to a gene detection chip and its matching reagents, more specifically to a gene detection chip for detecting a series of gene mutations closely related to the reactivity of malignant tumor treatment drugs And supporting reagents and their applications. Background technique [0002] Malignant tumors, as a major disease that plagues human physical and mental health, have become a global public health problem: According to the 2007 report of the World Health Organization, 6.3 million people died of cancer worldwide in 2006, accounting for about 12% of the total number of deaths. The second leading cause of death after cardiovascular disease. Since 1996, more than 10.3 million cancer patients have been newly diagnosed in the world every year. By the end of 1999, the total number of cancer patients in the world has exceeded 40 million. The World Health Organization reported in 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 周宏灏
Owner 湖南宏灏基因生物科技有限公司
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