Detection primer for banana wilt germina number-four biological strain and method for detecting same

A technique for detecting primers for Fusarium wilt of bananas, which is applied in the field of detection primers for plant pathogenic fungi, and can solve the problems of large-area outbreak loss and long survival period

Inactive Publication Date: 2008-01-30
SOUTH CHINA AGRI UNIV
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long survival period and rapid spread of the bacteria, there is no specific drug to prevent the disease. Therefore, once it occurs in banana production areas, it is likely that a large-scale outbreak will cause heavy losses.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection primer for banana wilt germina number-four biological strain and method for detecting same
  • Detection primer for banana wilt germina number-four biological strain and method for detecting same
  • Detection primer for banana wilt germina number-four biological strain and method for detecting same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Establishment of PCR system

[0033] Design the primer concentration, template, dNTPs, TaqDNA polymerase and PCR annealing temperature gradient to detect the effect of each reaction condition on the amplification effect, as follows:

[0034] Template DNA 1ul

[0035] 10×PCR Buffer 2.5ul

[0036] Mg 2+ (25mmol / l) 2.5ul

[0037] dNTPs (25mM) 2ul

[0038] Primer RF4-1 1ul

[0039] Primer RF4-2 1ul

[0040] Taq enzyme (5U / μL) 0.2ul

[0041] Sterilized ddH 2 O 14.8ul

[0042] Total volume 25ul

[0043] Prepare the reaction system on ice, mix well, and perform amplification according to the following PCR program: 94°C for 4min; 94°C for 30s, 52°C for 30s, 72°C for 45s, repeating 35 cycles; 72°C for 10min. Store at 4°C.

[0044] After the PCR reaction, 5 μl of the PCR product was taken for 1% agarose gel electrophoresis detection, and then the specific fragment was recovered, cloned, and sequenced to obtain the nucleic acid sequence of the specific fragment of th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the biological technical field and discloses a detection primer of plant pathogenic fungi and a detection method thereof. The invention adopts a special primer RF4 and optimizes action conditions to invent a PCR detection method for banana fusarium wilt physiological races (Race No. 4). The method can especially, sensitively and effectively detect banana fusarium wilt physiological races (Race No. 4) and mycelium even weighting 0.1 Mug can be detected and pathogen can even be detected from the root and the stem of bananas by adopting the method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection primer for plant pathogenic fungi and a detection method thereof. Background technique [0002] Banana fusarium wilt, also known as banana Panama disease and yellow leaf disease, is a soil-borne disease that causes vascular necrosis caused by Fusarium infection. The pathogen is Fusarium oxysporum f.sp.cubense( E.F. Smith) Snyder et Hansen] (FOC). The pathogen invades from the injured rhizome, develops to the stem through the host vascular bundle, and further spreads to the false leaves. When the plant dies, the pathogenic bacteria remains in the soil with the remains, and then spreads naturally in the banana orchard along with the water flow or the infected agricultural tools, and its chlamydospores can survive in the soil for 8-10 years. Because the bacteria has a long survival period and spreads rapidly, there is no specific drug to prevent the disease. Therefore, onc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/26
Inventor 王振中廖林凤
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap