46results about How to "Less reagent consumption" patented technology

The invention relates to a microfluidic cell array chip for high-throughput medicament screening, a preparation method thereof and use thereof. The microfluidic cell array chip for high-throughput medicament screening is characterized by sequentially comprising a valve-controlled channel layer, a fluid channel layer and a glass layer suitable for the anchorage growth of cells from top down. The chip can be used in high-throughput medicament screening by the parallel action of different medicament concentrations on various cells. The chip is manufactured by a multixposure one-development Su-8 negative photoresist process and multilayer PDMS bonding and has a structure with a high depth-to-width ratio. The microfluidic cell array chip provided by the invention can realize low reagent consumption and high-throughput co-culture of various cells, parallel analysis of the stimulation effect of different medicament concentrations on different cells and the real-time observation and detection of a slide of the cells and provide a fire-new technical platform and a method for high-throughput medicament screening and cell-medicament researches.

The present invention relates to the preparation process of low molecular weight seaweed polysaccharide sulfate. The preparation process includes adding ascorbic acid and hydrogen peroxide into the solution of natural seaweed polysaccharide sulfate as material, with the added amount being 0.1-50 mmol/L and 0.1-100 mmol/L and the ratio being 1 to 0.1-10, constant degradation under controlled temperature for 0.5-3 hr, dialysis or ultrafiltering, decompression concentration and freeze drying to obtain low molecular weight seaweed polysaccharide sulfate product of molecular weight 4-100 KDa. The present invention has the advantages of fast degradation speed, low reagent consumption, simple process, concentrated molecular weight distribution and other advantages. The low molecular weight seaweed polysaccharide sulfate product has controllable molecular weight, greatly raised dissolubility and bioactivity.

The invention provides a process for extracting vanadium by acid leaching of stone coal. The process mainly comprises the following steps of: removing aluminum from crystal aluminum sulfate ammonium vanadium obtained from acid leaching solution of stone coal, performing selective oxidation and adjustment of a pH value of the solution obtained after the aluminum removal to remove iron, performing oxidizing transformation of vanadium in the solution obtained after the iron removal, adsorbing the vanadium in the transformed solution by anion exchange resin, desorbing and regenerating the vanadium-loaded resin, performing ammonium salt precipitation of the vanadium-desorbing solution, performing pyrolysis of ammonium metavanadate, transforming the aluminum sulfate ammonium vanadium and the like. The process has the advantages of short flow, little consumption of reagent, high vanadium recovery rate, low production cost, good product quality and the like.

The invention discloses a method for extracting vanadium from stone coal pickle liquor. The technical process mainly comprises the steps of extracting the stone coal pickle liquor subjected to aluminum removal through an acidic phosphate ester extracting agent solvent to remove ferric iron, enriching the vanadium through the acidic phosphate ester extracting agent solvent after the iron is removed and the pH of the liquor is adjusted, neutralizing precipitates with the vanadium-enriched liquor to obtain vanadium dioxide hydrate, refining the vanadium dioxide hydrate, calcining the refined vanadium dioxide hydrate to obtain a vanadium oxide product and the like. The method for extracting the vanadium from stone coal pickle liquor has the advantages of short technical flow, low consumption of reagents, low production cost, high product quality, environment friendliness and the like.

The present invention discloses an extraction process of sesamin. Said process includes: firstly, purification process of sesamin: making sesame oil pass through the cylindrical body container whose interior is filled up with chromatographic adsorbent, at the same time making fat-soluble eluent, pass through the cylindrical body container to elute oily impurity, eluting until the bottom layer colour band of the cylindrical body container is removed; them making extraction process of sesamin: collecting adsorbent color band layer positioned in middle portion of the cylindrical body container, and utilizing solvent capable of dissolving sesamin to extract extract of sesamin containing in the color band layer. Said invention has the advantages of high extraction rate and high purity.

The invention discloses a determination method for trace impurity elements such as sodium, magnesium, calcium, iron and lead in high-purity boric acid. The determination method comprises the following specific steps of: heating hydrofluoric acid and hydrochloric acid to remove boron; extracting soluble salts by using aqua regia; setting constant volume by using ultrapure water; determining by an atomic absorption spectrometer and an inductively coupled plasma emission spectrometer; and performing quantitative enriching and assay determination on the trace impurity elements in a high-purity boric acid sample. According to the determination method, the boron is removed by the hydrofluoric acid; the using amount of reagents is small; the sample blank rate is low; sample computer analysis is facilitated by removing boric acid matrixes; and a single element and multiple elements can be simultaneously determined by means of atomic spectrums comprising the atomic absorption spectrometer and the inductively coupled plasma emission spectrometer. The determination method is suitable for the assay determination of the trace impurity elements in the high-purity boric acid sample and has the advantages of low detection limit, high sensitivity, wide linear range and the like. The average processing time of a single high-purity boric acid sample is not more than 2 hours.

The invention relates to a determination method for chloride ions of foods, which belongs to the technical field of food analysis. The method takes a chloride ion selective electrode as an indicator electrode and a two-liquid junction saturated calomel electrode as a reference electrode which are inserted in a sample solution to form a working battery, and when the concentration of chloride ions is in a range of 1 to 10-5g / L, the battery electromotive force is linear with the logarithm of chloride ion activity, and the concentration of the chloride ions of the foods is determined by a digital ion meter. The method of the invention adopts ultrasonic extraction and direct reading concentration method coupling techniques to determine the chlorine content in samples, and the used ultrasonic cleaning device greatly improves extraction speed; and the method has the obvious advantages of difficult sample contamination, low reagent consumption, simpleness and rapidity, accurate and reliable result.

The invention provides a complex tracer agent for oil field high-temperature hypersalinity block interwell monitoring and the application thereof. The tracer agent comprises one or more of chlorinated hydroxylamine erbium, hydroxylamine gadolinium chloride, chlorinated hydroxylamine dysprosium, chlorinated hydroxylamine ytterbium and chlorinated hydroxylamine holmium, and the tracer agent is formed by a tracer element and a complexing agent in a complexing mode. The tracer element is selected from one or more of erbium, gadolinium, dysprosium, ytterbium and holmium, the complexing agent is hydroxylammonium chloride, and the molar ratio between the tracer element and the complexing agent is 1:8-1:9 and preferably is 1:8. The tracer agent can resist high temperature (250 DEGC-300 DEG C) and hypersalinity (5000mg/L-20000mg/L). The invention further provides a tracer method for conducting the oil field high-temperature hypersalinity block interwell monitoring by means of the tracer agent, and the method comprises the process of conducting separation and enrichment on the tracer agent in a taken sample.

The invention provides a thiosulfate gold extraction method taking Fe (III) cyanide salts as oxidants. The method comprises the following steps: crushing and wet milling golden placer or mineral; adding solutions of Fe (III) cyanide salts, thiosulfate, and thiocarbamide into the ore pulp so as to ensure that in the whole system, the concentration of the thiosulfate is 0.01 to 2 mol/dm<3>, the concentration of the Fe (III) cyanide salts is 0.1 to 1.0 mmol/dm<3>, and the concentration of the thiocarbamide is 0.001 to 10.0 mmol/dm<3>; adjusting the pH value of the system to be 8 to 13; stirring and leaching for 9 to 24 hours; and recycling gold in the leachate according to a conventional method. The method has the advantages that the leaching rate is high; the process operation is simple and easy to control; the consumption of thiosulfate is low; the ingredients of the golden leachate are simple, so as to facilitate the recycling of gold; the application range is wide; and the purpose of environmental protection is realized.

The invention discloses a method for determining nanomolar active phosphate in seawater, and relates to a method for determining active phosphate with the concentration as nanomolar grade in the seawater. The invention provides the method for determining the nanomolar active phosphate in the seawater which is sensitive, accurate and quick, and is easy for field analysis on ships. The seawater with phosphate is added with a standard solution and is mixed with a reagent; water and a pre-washing solution are used to wash an enriching column after extraction and enrichment; then an extracted substance is eluted; a detector is used to detect at a position between 650 and 850nm; a corresponding signal is recorded; a working curve of the concentration of the phosphate and the corresponding signal is worked out; an actual seawater sample is mixed with the reagent, the water and the pre-washing solution are used to wash the enriching column after the extraction and the enrichment; then the extracted substance is eluted; the detector is used to detect at the position between 650 and 850nm; the corresponding signal is recorded; the standard working curve is used to work out the concentration of the phosphate in the seawater; and the determined concentration of the phosphate is the concentration of the active phosphate according to a definition of the active phosphate.

The invention relates to a method for analyzing trace phosphorus in ferromanganese, in particular to a method for quickly measuring phosphorus content in steelmaking raw material ferromanganese, which belongs to the technical field of chemical analysis test. The technical scheme comprises the following steps: taking a standard sample closing to an analysis sample as a working curve; after the sample is completely dissolved, analyzing by directly using an inductively coupled plasma atomic emission spectrometer; and dissolving the ferromanganese sample by using hydrochloric acid, nitric acid and perchloric acid. The invention has the beneficial effects of effectively solving the problems of complicated operation and slow analysis, reducing the reagent amount, reducing the environmental pollution and improving the analysis accuracy due to the adoption of high-precision analysis instrument. As the standard sample closing to the analysis sample is taken as the working curve, the influence caused by a matrix effect is eliminated. The result indicates that the error and precision completely conform to the technical requirements of the state standard. The result of analyzing a standard substance by using the invention is consistent with the determined value, and the invention can be used for measuring the trace phosphorus in high, middle and low-carbon ferromanganese.

The invention relates to a method for isolating magnesium and lithium from solution containing magnesium and lithium; the method comprises: adjusting pH of the solution containing magnesium and lithium, adding soluble phytate into the solution containing magnesium and lithium, so that Mg<2+> in the solution containing magnesium and lithium forms water-insoluble complex precipitate while Li<+> remains in the solution; after solid-liquid separation, concentrating the solution containing Li<+>, precipitating to obtain lithium carbonate, dissolving the magnesium-containing precipitate with hydrochloric acid, adsorbing phytate ions therein through weak alkalinity anions in weak acidity environment, and using the obtained solution containing Mg<2+> to prepare a magnesium salt; using NaOH solution to desorb the resin that loads phytate ions for the purpose of regeneration, and using the desorbed solution to precipitate Mg<2+> in solution containing magnesium and lithium in next cycle. The method has a short process, is simple to perform, and is efficient for isolating magnesium and lithium from the solution containing magnesium and lithium, the phytate is recyclable, the production cost is low, and the method is easily industrially applicable.

A glass chip mobile micro electrolytic cell includes the electrode, micro-electrolytic cell channel, solution channel, solution inlet and outlet, cover piece and substrate, electrode channel, electrode positioning ridge, electrode doors. On the substrate or on the substrate and the cover are synchronously etched with a micro electrolytic cell channel and electrode channel, the connection part of the two channels forms the electrode positioning ridge. Use glass lithography method and the heat integration to make chip usually. Electrodes are lead in from the side of the chip and positioned in the electrode channel. The volume of the micro mobile cell is Nano litre grade so the reagent consumption is low. The electrodes are separated without film because of lamina flow action of the channel. Advantages: dismounting and changing electrode is convenient, chip can be repeatedly used and electrode configuration is agility.

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO/GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP/GO/GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.

The invention relates to a droplet chip nucleic acid analysis system. The droplet chip nucleic acid analysis system comprises a mounting platform as a base mounting plane, a sample carrying bench forcarrying a nucleic acid analysis sample, a reagent carrying bench for carrying a nucleic acid analysis reagent, chip carrier carrying benches for carrying an open chip, a sampling needle that translates between the chip carrying benches, a temperature control module that controls the temperature of a chip, a magnetic control module that performs magnetic bead transferring on the contents of the chip, an optical detection module for performing nucleic acid analysis on the contents in the chip, and a signal acquisition module for performing signal acquisition on the optical detection module, Theoptical detection module is used to detect optical signals in the droplets of the nucleic acid analysis chip. The invention also relates to an analysis method of the droplet chip nucleic acid analysis system. The system has the advantages of small volume, low reagent consumption, flexible operation and fast analysis speed, and belongs to a medical device for biological detection.

The invention discloses a rapid aflatoxin B1 detecting method based on a smartphone detecting system.The rapid aflatoxin B1 detecting method includes the steps that aflatoxin B1 microchannels are prepared, detection reagents are added into the microchannels to prepare silver-stained PC sheet, aflatoxin-B1-detection optical hardware is designed through 3DMax software and printed through a 3D printer, the silver-stained PC sheet and a mobile phone are arranged into an optical hardware device, an aflatoxin B1 reaction area is photographed through a camera of the mobile phone, and the detection result of aflatoxin B1 is processed and displayed through a smartphone detection program.According to the rapid aflatoxin B1 detecting method, aflatoxin B1 is rapidly detected based on a smartphone, and the rapid aflatoxin B1 detecting method has the advantages of being convenient to carry, easy and convenient to operate, low in cost, high in flexibility and the like, and is suitable for rapid site detection.

The invention relates to a method for preparing high-purity anhydrous scandium acetate and high-purity scandium oxide. The method comprises the following steps: (a) adding strong acid into rough scandium oxide, heating to dissolve, and filtering to obtain filtrate I; (2) adding ammonium bicarbonate or sodium bicarbonate into the filtrate I under a stirring condition at a temperature maintained at 0-100 DEG C, stopping the adding when the pH value is 5-8, washing precipitates, and filtering, so as to obtain scandium carbonate precipitates; (c) adding acetic acid into the precipitates for dissolving, and filtering, so as to obtain filtrate II; (d) heating the filtrate II to a constant temperature of 70-100 DEG C, adding glacial acetic acid after crystals are separated, carrying out constant temperature treatment for 5-30 minutes, immediately filtering the crystals, collecting the crystals, and washing with absolute ethanol; (e) drying, so as to obtain high-purity anhydrous scandium acetate, and firing to obtain high-purity scandium oxide; and (f) firing high-purity anhydrous scandium acetate, or carrying out self-propagating combustion in an oxygen introduction condition, so as to obtain high-purity scandium oxide. The method has the advantages that the operation is simple and convenient, the environmental pollution is low, and the like.

The invention discloses a method for preparing an electronic cigarette liquid through microwave-assisted extraction. An extraction liquid and mixed tobacco leaves are mixed and irradiated by microwaves, organic phases are merged after filtering and washing operation, an organic solvent is finally removed, and a tobacco leaf extract is obtained, wherein the extraction liquid and the mixed tobacco leaves are mixed in the weight ratio being (1:1)-(5:1); the adopted power is 400-800 W during microwave irradiation; the faint-scent tobacco leaf extract is prepared from Yunnan Yuxi faint-scent tobacco leaves, Yunnan Dali faint-scent tobacco leaves and Yunnan Qujing faint-scent tobacco leaves. By comparison with conventional extraction methods such as a room-temperature extraction method and a heating reflux method, the extraction time is greatly shortened, the use quantity of solvents is reduced, and the extraction efficiency is remarkably improved. Besides, the extract is prepared from mixed tobacco leaf extracts, and different tobacco leaf extracts complement each other in scent, so that the scent and the taste of the electronic cigarette liquid are more close to those of conventional cigarettes.

The invention discloses a method for purifying ursodeoxycholic acid. The method comprises: 1) dissolving a ursodeoxycholic acid-containing mixture in tetrahydrofuran, adding diisopropylethylamine, andcarrying out a reaction to prepare a ursodeoxycholic acid diisopropylethylammonium salt-containing solution; 2) cooling the ursodeoxycholic acid diisopropylethylammonium salt-containing solution at 0-5 DEG C to crystallize the solution, and then performing separation to obtain ursodeoxycholic acid diisopropylethyl ammonium salt; and 3) hydrolyzing the ursodeoxycholic acid diisopropylethyl ammonium salt. The method has the advantages of simplicity in operation, low reagent consumption, high selectivity, and high purity of the obtained ursodeoxycholic acid.

The invention discloses a residual chlorine analysis method. The method comprises the following steps: adding a buffer solution into the to-be-detected sample to adjust the pH value; adding a DPD acidic salt color developing agent; uniform mixing, obtaining a first absorbance signal value V1 after the residual chlorine in the sample and the DPD acidic salt color developing agent are completely developed; and adding a strong oxidant into the completely developed solution to decompose a complex generated by the residual chlorine and the DPD acidic salt color developing agent to completely fade,so as to obtain a second absorbance signal value V2, substituting the V1-V2 values into the standard working curve, and calculating to obtain the concentration of the residual chlorine in the sample.According to the method, the influence of interference such as turbidity and chromaticity on residual chlorine determination is deducted by adopting an in-situ background interference deducting mode,the method is simple and easy to operate, low in reagent consumption and toxicity and particularly suitable for determining residual chlorine in samples with large chromaticity and turbidity and highinterference, and the reliability and accuracy of residual chlorine determination results can be effectively improved.

The invention provides an electrochemical method for extracting rubidium and cesium from brine. According to the method, on the basis of the selectivity of Prussian blue molecular space to rubidium and cesium ions, the rubidium and cesium ions are embedded and separated through electrochemical reduction and oxidation, and a highly-condensed liquid containing the rubidium and the cesium can be obtained, so that selective extraction of the rubidium and the cesium from the brine is realized. The method has the advantages of easiness in operation, small reagent use amount, high selective separation utilization rate and avoidance of production of waste water and waste residues.

The invention discloses a water quality detection pipeline system and a water quality detection method. The water quality detection pipeline system comprises a common channel, a waste liquid pool, a power mechanism, a quantitative detection mechanism and a multi-channel mechanism, wherein the multi-channel mechanism, the quantitative detection mechanism, the power mechanism and the waste liquid pool are sequentially arranged on the common channel, and an air pump is connected to the common channel between the power mechanism and the quantitative detection mechanism. According to the water quality detection pipeline system, a branch pipe on a multi-channel valve is connected with the atmosphere, air enters a pipeline at specific time through flow control, two sections of liquid generating cross contamination can be separated under the condition that quantification is not affected, the reagent consumption in the testing process is greatly reduced, and the problem that measurement is affected by cross contamination of reagents is solved.

The invention discloses a kit for detecting seminal gamma-L-glutamyltranspetidase and a detection method. The kit for detecting the seminal gamma-L-glutamyltranspetidase comprises alpha naphthylamine standard liquid, gamma-glutamoyl-alpha-naphthylamine matrix liquid and diazonium reagent. The detection method comprises the following steps of: setting parameters of a detection instrument; adding the matrix liquid into a blank tube, adding the standard liquid and the matrix liquid into a standard tube, and adding semen and the matrix liquid into a sample tube; simultaneously incubating the blank tube, the standard tube and the sample tube for 15 minutes at the temperature of 37 DEG C; and adding the diazonium reagent, mixing the diazonium reagent and the liquid uniformly, then incubating the tubes again for 5 minutes at 37 DEG C, and comparing colors through the detection instrument to obtain results. The kit and the detection method can simplify the operation, save the time and save the reagent; and the synchronous comparison experiments can be performed so that the results are stable and reliable, and the kit and the detection method are suitable for male laboratories of various hospitals.

The invention discloses a separation method of nickel in a sulfate solution. The separation method includes the steps of: adjusting the pH value of a solution after cobalt extraction to 7-10 via a complexing neutralizer, and extracting and separating nickel with 260# solvent oil as a diluent and a nickel extraction agent as an organic phase to obtain an organic phase supporting nickel and a sulfate raffinate; and washing and reversal-extracting the organic phase supporting nickel to prepare a reversal-extract containing nickel and an organic phase. The method can effectively separate nickel ina high-concentration sulfate solution, is low in energy and reagent consumption, has short operation process, is low in equipment demand and can easily achieve continuous operation.

The invention discloses a combinative processing method of high-concentration acrylic acid and ester wastewater, and belongs to the technical field of processing of high-concentration organic wastewater. The method provided by the invention adopts a combinative process of ultrasonic-method preprocessing, an anaerobic method, a two-stage aerobic method and ultrasonic-method deep processing for processing high-concentration acrylic acid and ester wastewater with a COD (Chemical Oxygen Demand) value larger than 40,000mg/L, wherein the ultrasonic-method preprocessing is carried out by adopting two stages; the relatively-high-frequency processing is first adopted; afterwards, the low-frequency processing is adopted; processed outlet water has a COD smaller than 60mg/L and is up to the national grade-1 discharge standard in Integrated Wastewater Discharge Standard (GB8978-1996).

The invention relates to the technical field of oil field exploration and development, in particular to a gas chromatography rapid quantitative analysis method for saturated hydrocarbon in a crude oilsample. The method comprises the following steps: step 1, preparing a standard substance solution; 2, weighing a proper amount of crude oil sample; 3, adding a standard sample and a reagent into thecrude oil sample to obtain a crude oil detection solution; 4, putting the crude oil detection solution into a sample bottle, putting the sample bottle into an ultrasonic cleaner, oscillating and uniformly mixing; step 5, putting the sample bottle into a centrifugal machine for centrifugal separation; and 6, carrying out gas chromatographic analysis on the centrifugally separated crude oil detection solution, and carrying out quantitative analysis. According to the method disclosed by the invention, chromatographic analysis can be carried out on the crude oil sample without chromatographic column treatment, so that the operation steps are simplified, the reagent consumption is reduced, the analysis process is shortened, and analysis data can be quickly provided.

The invention discloses a fluorescence sensation method for detecting exonuclease I (Exo I). The fluorescence sensation method is characterized by comprising the following specific steps: (1) mixing a cation conjugated polymer (CCP) with an iridium complex, further adding an Exo I buffer solution so as to obtain a fluorescence sensor system for detecting Exo I and based on a fluorescence resonance energy transfer (FRET) effect of the CCP and the iridium complex, and detecting fluorescence intensity; (2) adding single-chain DNA (ssDNA) into the system of the step (1), and detecting variation of the fluorescence intensity; (3) adding Exo I of different concentrations into the step (2), and detecting activity of the Exo I in a sample. The fluorescence sensation method has the advantage that an analysis sensation method which is good in specificity, high in sensitivity, rapid in detection speed and accurate and reliable in result for Exo I detection is developed for a first time.

The invention provides a method for detecting the content of poa pratensis fatty acid. The method comprises the following steps of: (1) taking poa pratensis leaf tissues in a glass bottle; (2) adding sulfuric acid containing daturic acid; (3) discharging air in the glass bottle and sealing to perform water bath or metal bath; (4) cooling, and adding sodium chloride solution and normal hexane to whirl and centrifuge; (5) taking the centrifuged upper oil phase and putting into a gas chromatography-mass spectrometer (GC-MS) to analyze, and calculating the content of fatty acid according to the sample peak diagram. The method provided by the invention overcomes the problem of inconsistent extracting efficiency of lipids among the samples, optimizes the plant tissue sample amount of the sample to be detected and saves the dosage of a series of reagent in the process of methyl esterification; furthermore, the operation step of methyl esterification is simplified, the detecting and analyzing time of GC-MS after sampling is saved; the problem of serious carbonization in the process of the methyl esterification of the poa pratensis is overcome; therefore, the method provided by the invention is a poa pratensis fatty acid analyzing method with low cost, fast speed, simplicity and high efficiency.

The invention relates to a method of simultaneously evaluating the oxidation resistance and the contents of the active constituents of gallic acid and ellagic acid in a downy rosemyrtle root by usingDPPH-UPLC. The method comprises the following steps: a to-be-detected sample before and after reaction with the DPPH is subjected to ultra-high performance liquid chromatography detection; and based on gallic acid and ellagic acid peak areas of a test solution before and after DPPH reaction detected by UPLC, gallic acid and ellagic acid contents and clearance rates are calculated. The oxidation resistance and the contents of the active constituents in the downy rosemyrtle root can be detected at the same time, the operation is simple, quick and convenient, the consumption of a reagent is little, the sensitivity is high, the oxidation resistance active constituents and the contents in the downy rosemyrtle root can be evaluated, and a theoretical basis is provided for screening and further development of the downy rosemyrtle oxidation resistance active constituents.

The invention discloses a nano fluorescent probe as well as a preparation method and application thereof. The nano fluorescent probe is prepared by the following method of taking ascorbic acid as a precursor, taking water and ethanol as solvents, and adopting a hydrothermal method. The nano fluorescent probe is used for detecting uric acid. The invention provides a carbon quantum dot (carbon dot for short) for uric acid detection, and discloses a specific uric acid detection method, which combines a Fenton reaction catalyzed by ferrous ions to catalyze decomposition of hydrogen peroxide generated after uric acid oxidation to generate reactive oxygen species, so that the fluorescence intensity of the carbon quantum dot is quenched, and the effective detection of uric acid concentration is thus realized. The method has the advantages of simplicity and convenience in operation, low reagent dosage, high detection speed, high accuracy, good selectivity and the like; the carbon dots have the advantages of being simple in preparation method, good in water solubility, high in pH stability and the like, and large-scale production can be achieved.