67results about How to "Less reagent consumption" patented technology

The invention relates to a microfluidic cell array chip for high-throughput medicament screening, a preparation method thereof and use thereof. The microfluidic cell array chip for high-throughput medicament screening is characterized by sequentially comprising a valve-controlled channel layer, a fluid channel layer and a glass layer suitable for the anchorage growth of cells from top down. The chip can be used in high-throughput medicament screening by the parallel action of different medicament concentrations on various cells. The chip is manufactured by a multixposure one-development Su-8 negative photoresist process and multilayer PDMS bonding and has a structure with a high depth-to-width ratio. The microfluidic cell array chip provided by the invention can realize low reagent consumption and high-throughput co-culture of various cells, parallel analysis of the stimulation effect of different medicament concentrations on different cells and the real-time observation and detection of a slide of the cells and provide a fire-new technical platform and a method for high-throughput medicament screening and cell-medicament researches.

The present invention relates to the preparation process of low molecular weight seaweed polysaccharide sulfate. The preparation process includes adding ascorbic acid and hydrogen peroxide into the solution of natural seaweed polysaccharide sulfate as material, with the added amount being 0.1-50 mmol/L and 0.1-100 mmol/L and the ratio being 1 to 0.1-10, constant degradation under controlled temperature for 0.5-3 hr, dialysis or ultrafiltering, decompression concentration and freeze drying to obtain low molecular weight seaweed polysaccharide sulfate product of molecular weight 4-100 KDa. The present invention has the advantages of fast degradation speed, low reagent consumption, simple process, concentrated molecular weight distribution and other advantages. The low molecular weight seaweed polysaccharide sulfate product has controllable molecular weight, greatly raised dissolubility and bioactivity.

The invention provides a process for extracting vanadium by acid leaching of stone coal. The process mainly comprises the following steps of: removing aluminum from crystal aluminum sulfate ammonium vanadium obtained from acid leaching solution of stone coal, performing selective oxidation and adjustment of a pH value of the solution obtained after the aluminum removal to remove iron, performing oxidizing transformation of vanadium in the solution obtained after the iron removal, adsorbing the vanadium in the transformed solution by anion exchange resin, desorbing and regenerating the vanadium-loaded resin, performing ammonium salt precipitation of the vanadium-desorbing solution, performing pyrolysis of ammonium metavanadate, transforming the aluminum sulfate ammonium vanadium and the like. The process has the advantages of short flow, little consumption of reagent, high vanadium recovery rate, low production cost, good product quality and the like.

The invention discloses a method for extracting vanadium from stone coal pickle liquor. The technical process mainly comprises the steps of extracting the stone coal pickle liquor subjected to aluminum removal through an acidic phosphate ester extracting agent solvent to remove ferric iron, enriching the vanadium through the acidic phosphate ester extracting agent solvent after the iron is removed and the pH of the liquor is adjusted, neutralizing precipitates with the vanadium-enriched liquor to obtain vanadium dioxide hydrate, refining the vanadium dioxide hydrate, calcining the refined vanadium dioxide hydrate to obtain a vanadium oxide product and the like. The method for extracting the vanadium from stone coal pickle liquor has the advantages of short technical flow, low consumption of reagents, low production cost, high product quality, environment friendliness and the like.

The present invention discloses an extraction process of sesamin. Said process includes: firstly, purification process of sesamin: making sesame oil pass through the cylindrical body container whose interior is filled up with chromatographic adsorbent, at the same time making fat-soluble eluent, pass through the cylindrical body container to elute oily impurity, eluting until the bottom layer colour band of the cylindrical body container is removed; them making extraction process of sesamin: collecting adsorbent color band layer positioned in middle portion of the cylindrical body container, and utilizing solvent capable of dissolving sesamin to extract extract of sesamin containing in the color band layer. Said invention has the advantages of high extraction rate and high purity.

The invention discloses a determination method for trace impurity elements such as sodium, magnesium, calcium, iron and lead in high-purity boric acid. The determination method comprises the following specific steps of: heating hydrofluoric acid and hydrochloric acid to remove boron; extracting soluble salts by using aqua regia; setting constant volume by using ultrapure water; determining by an atomic absorption spectrometer and an inductively coupled plasma emission spectrometer; and performing quantitative enriching and assay determination on the trace impurity elements in a high-purity boric acid sample. According to the determination method, the boron is removed by the hydrofluoric acid; the using amount of reagents is small; the sample blank rate is low; sample computer analysis is facilitated by removing boric acid matrixes; and a single element and multiple elements can be simultaneously determined by means of atomic spectrums comprising the atomic absorption spectrometer and the inductively coupled plasma emission spectrometer. The determination method is suitable for the assay determination of the trace impurity elements in the high-purity boric acid sample and has the advantages of low detection limit, high sensitivity, wide linear range and the like. The average processing time of a single high-purity boric acid sample is not more than 2 hours.

Disclosed is a microfluidic chip used for cell co-culture and a cell culture method thereof. The microfluidic chip comprises successively from bottom to top: a basal layer, a cell culture chamber layer and an upper cover plate layer. A bolting cloth is arranged in the cell culture chamber layer to divide cell culture chambers into two layers, the upper layer is a culture medium exchanging layer, and the lower layer is a cell culture layer. A microfiltration membrane is arranged between the adjacent cell culture chambers. One or more upper cover plate through holes, corresponding to each cell culture chamber, are formed in the upper cover plate layer, and the through holes mutually correspond to bolting cloth through holes in position. Connectors are arranged in the positions corresponding to the upper cover plate through holes. A pipeline used for culture medium exchanging and cell inoculating or cell detecting is arranged in each connector. The microfluidic chip used for cell co-culture can intercept and retain suspension cells effectively, so that microfluidic cell co-culture of adherent cells and suspension cells or suspension cells and adherent cells is achieved.

The invention relates to a determination method for chloride ions of foods, which belongs to the technical field of food analysis. The method takes a chloride ion selective electrode as an indicator electrode and a two-liquid junction saturated calomel electrode as a reference electrode which are inserted in a sample solution to form a working battery, and when the concentration of chloride ions is in a range of 1 to 10-5g / L, the battery electromotive force is linear with the logarithm of chloride ion activity, and the concentration of the chloride ions of the foods is determined by a digital ion meter. The method of the invention adopts ultrasonic extraction and direct reading concentration method coupling techniques to determine the chlorine content in samples, and the used ultrasonic cleaning device greatly improves extraction speed; and the method has the obvious advantages of difficult sample contamination, low reagent consumption, simpleness and rapidity, accurate and reliable result.

The invention provides a complex tracer agent for oil field high-temperature hypersalinity block interwell monitoring and the application thereof. The tracer agent comprises one or more of chlorinated hydroxylamine erbium, hydroxylamine gadolinium chloride, chlorinated hydroxylamine dysprosium, chlorinated hydroxylamine ytterbium and chlorinated hydroxylamine holmium, and the tracer agent is formed by a tracer element and a complexing agent in a complexing mode. The tracer element is selected from one or more of erbium, gadolinium, dysprosium, ytterbium and holmium, the complexing agent is hydroxylammonium chloride, and the molar ratio between the tracer element and the complexing agent is 1:8-1:9 and preferably is 1:8. The tracer agent can resist high temperature (250 DEGC-300 DEG C) and hypersalinity (5000mg/L-20000mg/L). The invention further provides a tracer method for conducting the oil field high-temperature hypersalinity block interwell monitoring by means of the tracer agent, and the method comprises the process of conducting separation and enrichment on the tracer agent in a taken sample.

The invention provides a thiosulfate gold extraction method taking Fe (III) cyanide salts as oxidants. The method comprises the following steps: crushing and wet milling golden placer or mineral; adding solutions of Fe (III) cyanide salts, thiosulfate, and thiocarbamide into the ore pulp so as to ensure that in the whole system, the concentration of the thiosulfate is 0.01 to 2 mol/dm<3>, the concentration of the Fe (III) cyanide salts is 0.1 to 1.0 mmol/dm<3>, and the concentration of the thiocarbamide is 0.001 to 10.0 mmol/dm<3>; adjusting the pH value of the system to be 8 to 13; stirring and leaching for 9 to 24 hours; and recycling gold in the leachate according to a conventional method. The method has the advantages that the leaching rate is high; the process operation is simple and easy to control; the consumption of thiosulfate is low; the ingredients of the golden leachate are simple, so as to facilitate the recycling of gold; the application range is wide; and the purpose of environmental protection is realized.

The invention discloses a method for determining nanomolar active phosphate in seawater, and relates to a method for determining active phosphate with the concentration as nanomolar grade in the seawater. The invention provides the method for determining the nanomolar active phosphate in the seawater which is sensitive, accurate and quick, and is easy for field analysis on ships. The seawater with phosphate is added with a standard solution and is mixed with a reagent; water and a pre-washing solution are used to wash an enriching column after extraction and enrichment; then an extracted substance is eluted; a detector is used to detect at a position between 650 and 850nm; a corresponding signal is recorded; a working curve of the concentration of the phosphate and the corresponding signal is worked out; an actual seawater sample is mixed with the reagent, the water and the pre-washing solution are used to wash the enriching column after the extraction and the enrichment; then the extracted substance is eluted; the detector is used to detect at the position between 650 and 850nm; the corresponding signal is recorded; the standard working curve is used to work out the concentration of the phosphate in the seawater; and the determined concentration of the phosphate is the concentration of the active phosphate according to a definition of the active phosphate.

The invention discloses a method for detecting sulfonamide and beta-lactam antibiotics in livestock and poultry breeding wastewater. The method comprises the following steps: sequentially centrifugingand filtering a to-be-detected water sample; adding deionized water into the obtained filtrate for dilution; adding Na2EDTA, adjusting the pH, carrying out solid-phase extraction treatment on the obtained pre-treated water sample, carrying out negative-pressure leaching treatment and normal-pressure elution treatment on a solid-phase extraction column subjected to the solid-phase extraction treatment, collecting eluent, and then carrying out nitrogen blowing and constant volume treatment to obtain an antibiotic solution to be detected; and carrying out antibiotic content detection on the antibiotic solution to be detected by adopting a high performance liquid chromatography-tandem mass spectrometry method. According to the detection method disclosed by the invention, a solid phase extraction-liquid chromatography tandem mass spectrometry method is adopted, and various process conditions and parameters are selected and determined, so that the detection method can be used for rapidly and accurately detecting antibiotics in water and detecting sulfonamide and beta-lactam antibiotics in the livestock and poultry breeding wastewater at the same time.

The invention relates to a method for analyzing trace phosphorus in ferromanganese, in particular to a method for quickly measuring phosphorus content in steelmaking raw material ferromanganese, which belongs to the technical field of chemical analysis test. The technical scheme comprises the following steps: taking a standard sample closing to an analysis sample as a working curve; after the sample is completely dissolved, analyzing by directly using an inductively coupled plasma atomic emission spectrometer; and dissolving the ferromanganese sample by using hydrochloric acid, nitric acid and perchloric acid. The invention has the beneficial effects of effectively solving the problems of complicated operation and slow analysis, reducing the reagent amount, reducing the environmental pollution and improving the analysis accuracy due to the adoption of high-precision analysis instrument. As the standard sample closing to the analysis sample is taken as the working curve, the influence caused by a matrix effect is eliminated. The result indicates that the error and precision completely conform to the technical requirements of the state standard. The result of analyzing a standard substance by using the invention is consistent with the determined value, and the invention can be used for measuring the trace phosphorus in high, middle and low-carbon ferromanganese.

The invention discloses a method for detecting NO (nitric oxide) content in exhaled gas of a human body, wherein PTIO (3-oxo-2-phenyl-4,4,5,5-tetramethyl imidazoline-1-oxygen) is utilized as an oxidizing agent of the NO, and the content of the NO in the exhaled gas of the human body can be directly and quickly detected by adopting an electrospray ionization extraction mass spectrometry method. The method disclosed by the invention has strong specificity and high sensitivity without pre-treatment on a sample; the PTIO has better stability and reaction sensitivity, and can selectively react with the NO. In addition, the method is integrated with sample collection and treatment, so that the consumed dose is small, the operation is convenient and simple and the detection can be finished in a short time. The method has the advantages of high mass spectrum sensitivity and good stability, breaks through the disadvantage of a traditional method, and realizes fast and sensitive monitoring of the NO in the exhaled gas of the human body.

The invention relates to a method for isolating magnesium and lithium from solution containing magnesium and lithium; the method comprises: adjusting pH of the solution containing magnesium and lithium, adding soluble phytate into the solution containing magnesium and lithium, so that Mg<2+> in the solution containing magnesium and lithium forms water-insoluble complex precipitate while Li<+> remains in the solution; after solid-liquid separation, concentrating the solution containing Li<+>, precipitating to obtain lithium carbonate, dissolving the magnesium-containing precipitate with hydrochloric acid, adsorbing phytate ions therein through weak alkalinity anions in weak acidity environment, and using the obtained solution containing Mg<2+> to prepare a magnesium salt; using NaOH solution to desorb the resin that loads phytate ions for the purpose of regeneration, and using the desorbed solution to precipitate Mg<2+> in solution containing magnesium and lithium in next cycle. The method has a short process, is simple to perform, and is efficient for isolating magnesium and lithium from the solution containing magnesium and lithium, the phytate is recyclable, the production cost is low, and the method is easily industrially applicable.

A glass chip mobile micro electrolytic cell includes the electrode, micro-electrolytic cell channel, solution channel, solution inlet and outlet, cover piece and substrate, electrode channel, electrode positioning ridge, electrode doors. On the substrate or on the substrate and the cover are synchronously etched with a micro electrolytic cell channel and electrode channel, the connection part of the two channels forms the electrode positioning ridge. Use glass lithography method and the heat integration to make chip usually. Electrodes are lead in from the side of the chip and positioned in the electrode channel. The volume of the micro mobile cell is Nano litre grade so the reagent consumption is low. The electrodes are separated without film because of lamina flow action of the channel. Advantages: dismounting and changing electrode is convenient, chip can be repeatedly used and electrode configuration is agility.

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO/GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP/GO/GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.

The invention relates to a droplet chip nucleic acid analysis system. The droplet chip nucleic acid analysis system comprises a mounting platform as a base mounting plane, a sample carrying bench forcarrying a nucleic acid analysis sample, a reagent carrying bench for carrying a nucleic acid analysis reagent, chip carrier carrying benches for carrying an open chip, a sampling needle that translates between the chip carrying benches, a temperature control module that controls the temperature of a chip, a magnetic control module that performs magnetic bead transferring on the contents of the chip, an optical detection module for performing nucleic acid analysis on the contents in the chip, and a signal acquisition module for performing signal acquisition on the optical detection module, Theoptical detection module is used to detect optical signals in the droplets of the nucleic acid analysis chip. The invention also relates to an analysis method of the droplet chip nucleic acid analysis system. The system has the advantages of small volume, low reagent consumption, flexible operation and fast analysis speed, and belongs to a medical device for biological detection.

The invention discloses a rapid aflatoxin B1 detecting method based on a smartphone detecting system.The rapid aflatoxin B1 detecting method includes the steps that aflatoxin B1 microchannels are prepared, detection reagents are added into the microchannels to prepare silver-stained PC sheet, aflatoxin-B1-detection optical hardware is designed through 3DMax software and printed through a 3D printer, the silver-stained PC sheet and a mobile phone are arranged into an optical hardware device, an aflatoxin B1 reaction area is photographed through a camera of the mobile phone, and the detection result of aflatoxin B1 is processed and displayed through a smartphone detection program.According to the rapid aflatoxin B1 detecting method, aflatoxin B1 is rapidly detected based on a smartphone, and the rapid aflatoxin B1 detecting method has the advantages of being convenient to carry, easy and convenient to operate, low in cost, high in flexibility and the like, and is suitable for rapid site detection.

The invention relates to a method for preparing high-purity anhydrous scandium acetate and high-purity scandium oxide. The method comprises the following steps: (a) adding strong acid into rough scandium oxide, heating to dissolve, and filtering to obtain filtrate I; (2) adding ammonium bicarbonate or sodium bicarbonate into the filtrate I under a stirring condition at a temperature maintained at 0-100 DEG C, stopping the adding when the pH value is 5-8, washing precipitates, and filtering, so as to obtain scandium carbonate precipitates; (c) adding acetic acid into the precipitates for dissolving, and filtering, so as to obtain filtrate II; (d) heating the filtrate II to a constant temperature of 70-100 DEG C, adding glacial acetic acid after crystals are separated, carrying out constant temperature treatment for 5-30 minutes, immediately filtering the crystals, collecting the crystals, and washing with absolute ethanol; (e) drying, so as to obtain high-purity anhydrous scandium acetate, and firing to obtain high-purity scandium oxide; and (f) firing high-purity anhydrous scandium acetate, or carrying out self-propagating combustion in an oxygen introduction condition, so as to obtain high-purity scandium oxide. The method has the advantages that the operation is simple and convenient, the environmental pollution is low, and the like.

The invention discloses a method for preparing an electronic cigarette liquid through microwave-assisted extraction. An extraction liquid and mixed tobacco leaves are mixed and irradiated by microwaves, organic phases are merged after filtering and washing operation, an organic solvent is finally removed, and a tobacco leaf extract is obtained, wherein the extraction liquid and the mixed tobacco leaves are mixed in the weight ratio being (1:1)-(5:1); the adopted power is 400-800 W during microwave irradiation; the faint-scent tobacco leaf extract is prepared from Yunnan Yuxi faint-scent tobacco leaves, Yunnan Dali faint-scent tobacco leaves and Yunnan Qujing faint-scent tobacco leaves. By comparison with conventional extraction methods such as a room-temperature extraction method and a heating reflux method, the extraction time is greatly shortened, the use quantity of solvents is reduced, and the extraction efficiency is remarkably improved. Besides, the extract is prepared from mixed tobacco leaf extracts, and different tobacco leaf extracts complement each other in scent, so that the scent and the taste of the electronic cigarette liquid are more close to those of conventional cigarettes.

The invention discloses a method for purifying ursodeoxycholic acid. The method comprises: 1) dissolving a ursodeoxycholic acid-containing mixture in tetrahydrofuran, adding diisopropylethylamine, andcarrying out a reaction to prepare a ursodeoxycholic acid diisopropylethylammonium salt-containing solution; 2) cooling the ursodeoxycholic acid diisopropylethylammonium salt-containing solution at 0-5 DEG C to crystallize the solution, and then performing separation to obtain ursodeoxycholic acid diisopropylethyl ammonium salt; and 3) hydrolyzing the ursodeoxycholic acid diisopropylethyl ammonium salt. The method has the advantages of simplicity in operation, low reagent consumption, high selectivity, and high purity of the obtained ursodeoxycholic acid.

The invention discloses a residual chlorine analysis method. The method comprises the following steps: adding a buffer solution into the to-be-detected sample to adjust the pH value; adding a DPD acidic salt color developing agent; uniform mixing, obtaining a first absorbance signal value V1 after the residual chlorine in the sample and the DPD acidic salt color developing agent are completely developed; and adding a strong oxidant into the completely developed solution to decompose a complex generated by the residual chlorine and the DPD acidic salt color developing agent to completely fade,so as to obtain a second absorbance signal value V2, substituting the V1-V2 values into the standard working curve, and calculating to obtain the concentration of the residual chlorine in the sample.According to the method, the influence of interference such as turbidity and chromaticity on residual chlorine determination is deducted by adopting an in-situ background interference deducting mode,the method is simple and easy to operate, low in reagent consumption and toxicity and particularly suitable for determining residual chlorine in samples with large chromaticity and turbidity and highinterference, and the reliability and accuracy of residual chlorine determination results can be effectively improved.