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46results about How to "Less reagent consumption" patented technology

Determination method for trace impurity elements such as sodium, magnesium, calcium, iron and lead in high-purity boric acid

The invention discloses a determination method for trace impurity elements such as sodium, magnesium, calcium, iron and lead in high-purity boric acid. The determination method comprises the following specific steps of: heating hydrofluoric acid and hydrochloric acid to remove boron; extracting soluble salts by using aqua regia; setting constant volume by using ultrapure water; determining by an atomic absorption spectrometer and an inductively coupled plasma emission spectrometer; and performing quantitative enriching and assay determination on the trace impurity elements in a high-purity boric acid sample. According to the determination method, the boron is removed by the hydrofluoric acid; the using amount of reagents is small; the sample blank rate is low; sample computer analysis is facilitated by removing boric acid matrixes; and a single element and multiple elements can be simultaneously determined by means of atomic spectrums comprising the atomic absorption spectrometer and the inductively coupled plasma emission spectrometer. The determination method is suitable for the assay determination of the trace impurity elements in the high-purity boric acid sample and has the advantages of low detection limit, high sensitivity, wide linear range and the like. The average processing time of a single high-purity boric acid sample is not more than 2 hours.
Owner:INST OF MULTIPURPOSE UTILIZATION OF MINERAL RESOURCES CHINESE ACAD OF GEOLOGICAL SCI

Method for measuring nanomolar reactive phosphate in sea water

The invention discloses a method for determining nanomolar active phosphate in seawater, and relates to a method for determining active phosphate with the concentration as nanomolar grade in the seawater. The invention provides the method for determining the nanomolar active phosphate in the seawater which is sensitive, accurate and quick, and is easy for field analysis on ships. The seawater with phosphate is added with a standard solution and is mixed with a reagent; water and a pre-washing solution are used to wash an enriching column after extraction and enrichment; then an extracted substance is eluted; a detector is used to detect at a position between 650 and 850nm; a corresponding signal is recorded; a working curve of the concentration of the phosphate and the corresponding signal is worked out; an actual seawater sample is mixed with the reagent, the water and the pre-washing solution are used to wash the enriching column after the extraction and the enrichment; then the extracted substance is eluted; the detector is used to detect at the position between 650 and 850nm; the corresponding signal is recorded; the standard working curve is used to work out the concentration of the phosphate in the seawater; and the determined concentration of the phosphate is the concentration of the active phosphate according to a definition of the active phosphate.
Owner:XIAMEN UNIV

Method for analyzing trace phosphorus in ferromanganese

The invention relates to a method for analyzing trace phosphorus in ferromanganese, in particular to a method for quickly measuring phosphorus content in steelmaking raw material ferromanganese, which belongs to the technical field of chemical analysis test. The technical scheme comprises the following steps: taking a standard sample closing to an analysis sample as a working curve; after the sample is completely dissolved, analyzing by directly using an inductively coupled plasma atomic emission spectrometer; and dissolving the ferromanganese sample by using hydrochloric acid, nitric acid and perchloric acid. The invention has the beneficial effects of effectively solving the problems of complicated operation and slow analysis, reducing the reagent amount, reducing the environmental pollution and improving the analysis accuracy due to the adoption of high-precision analysis instrument. As the standard sample closing to the analysis sample is taken as the working curve, the influence caused by a matrix effect is eliminated. The result indicates that the error and precision completely conform to the technical requirements of the state standard. The result of analyzing a standard substance by using the invention is consistent with the determined value, and the invention can be used for measuring the trace phosphorus in high, middle and low-carbon ferromanganese.
Owner:TANGSHAN JIANLONG IND CO LTD

Electrochemical biosensor based on polypeptide analog with electrocatalytic activity for acetylcholin esterase detection

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO/GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP/GO/GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.
Owner:NINGBO UNIV

Method for preparing high-purity anhydrous scandium acetate and high-purity scandium oxide

The invention relates to a method for preparing high-purity anhydrous scandium acetate and high-purity scandium oxide. The method comprises the following steps: (a) adding strong acid into rough scandium oxide, heating to dissolve, and filtering to obtain filtrate I; (2) adding ammonium bicarbonate or sodium bicarbonate into the filtrate I under a stirring condition at a temperature maintained at 0-100 DEG C, stopping the adding when the pH value is 5-8, washing precipitates, and filtering, so as to obtain scandium carbonate precipitates; (c) adding acetic acid into the precipitates for dissolving, and filtering, so as to obtain filtrate II; (d) heating the filtrate II to a constant temperature of 70-100 DEG C, adding glacial acetic acid after crystals are separated, carrying out constant temperature treatment for 5-30 minutes, immediately filtering the crystals, collecting the crystals, and washing with absolute ethanol; (e) drying, so as to obtain high-purity anhydrous scandium acetate, and firing to obtain high-purity scandium oxide; and (f) firing high-purity anhydrous scandium acetate, or carrying out self-propagating combustion in an oxygen introduction condition, so as to obtain high-purity scandium oxide. The method has the advantages that the operation is simple and convenient, the environmental pollution is low, and the like.
Owner:YIYANG HONGYUAN RARE EARTH

Residual chlorine analysis method

The invention discloses a residual chlorine analysis method. The method comprises the following steps: adding a buffer solution into the to-be-detected sample to adjust the pH value; adding a DPD acidic salt color developing agent; uniform mixing, obtaining a first absorbance signal value V1 after the residual chlorine in the sample and the DPD acidic salt color developing agent are completely developed; and adding a strong oxidant into the completely developed solution to decompose a complex generated by the residual chlorine and the DPD acidic salt color developing agent to completely fade,so as to obtain a second absorbance signal value V2, substituting the V1-V2 values into the standard working curve, and calculating to obtain the concentration of the residual chlorine in the sample.According to the method, the influence of interference such as turbidity and chromaticity on residual chlorine determination is deducted by adopting an in-situ background interference deducting mode,the method is simple and easy to operate, low in reagent consumption and toxicity and particularly suitable for determining residual chlorine in samples with large chromaticity and turbidity and highinterference, and the reliability and accuracy of residual chlorine determination results can be effectively improved.
Owner:LIHE TECH (HUNAN) CO LTD

Kit for detecting seminal gamma-L-glutamyltranspetidase and detection method

The invention discloses a kit for detecting seminal gamma-L-glutamyltranspetidase and a detection method. The kit for detecting the seminal gamma-L-glutamyltranspetidase comprises alpha naphthylamine standard liquid, gamma-glutamoyl-alpha-naphthylamine matrix liquid and diazonium reagent. The detection method comprises the following steps of: setting parameters of a detection instrument; adding the matrix liquid into a blank tube, adding the standard liquid and the matrix liquid into a standard tube, and adding semen and the matrix liquid into a sample tube; simultaneously incubating the blank tube, the standard tube and the sample tube for 15 minutes at the temperature of 37 DEG C; and adding the diazonium reagent, mixing the diazonium reagent and the liquid uniformly, then incubating the tubes again for 5 minutes at 37 DEG C, and comparing colors through the detection instrument to obtain results. The kit and the detection method can simplify the operation, save the time and save the reagent; and the synchronous comparison experiments can be performed so that the results are stable and reliable, and the kit and the detection method are suitable for male laboratories of various hospitals.
Owner:南京欣迪生物药业工程有限责任公司

Method for detecting content of poa pratensis fatty acid

InactiveCN102706974AOptimizing Plant Tissue Sample VolumeLess reagent consumptionComponent separationChemistryLawn
The invention provides a method for detecting the content of poa pratensis fatty acid. The method comprises the following steps of: (1) taking poa pratensis leaf tissues in a glass bottle; (2) adding sulfuric acid containing daturic acid; (3) discharging air in the glass bottle and sealing to perform water bath or metal bath; (4) cooling, and adding sodium chloride solution and normal hexane to whirl and centrifuge; (5) taking the centrifuged upper oil phase and putting into a gas chromatography-mass spectrometer (GC-MS) to analyze, and calculating the content of fatty acid according to the sample peak diagram. The method provided by the invention overcomes the problem of inconsistent extracting efficiency of lipids among the samples, optimizes the plant tissue sample amount of the sample to be detected and saves the dosage of a series of reagent in the process of methyl esterification; furthermore, the operation step of methyl esterification is simplified, the detecting and analyzing time of GC-MS after sampling is saved; the problem of serious carbonization in the process of the methyl esterification of the poa pratensis is overcome; therefore, the method provided by the invention is a poa pratensis fatty acid analyzing method with low cost, fast speed, simplicity and high efficiency.
Owner:BEIJING FORESTRY UNIVERSITY
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