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Droplet chip nucleic acid analysis system and analysis method thereof

A nucleic acid analysis and chip technology, applied in the field of medical devices, can solve the problems of high processing cost, complex control system, and difficult to popularize, and achieve the effects of fast analysis, flexible application, and avoidance of cross-contamination.

Active Publication Date: 2018-11-16
广州市第一人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a series of chip micropump and microvalve technologies have been reported before, they are difficult to popularize due to high processing costs and complex control systems.

Method used

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  • Droplet chip nucleic acid analysis system and analysis method thereof
  • Droplet chip nucleic acid analysis system and analysis method thereof
  • Droplet chip nucleic acid analysis system and analysis method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A droplet chip nucleic acid analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying nucleic acid analysis samples, a reagent carrier platform for nucleic acid analysis reagents, a chip carrier platform for open chips, and a sample carrier platform , Reagent carrier, sampling needle for translation between chip carriers, temperature control module for temperature control of the chip, magnetic control module for magnetic bead transfer of the contents in the chip, nucleic acid analysis for the contents of the chip An optical detection module, a signal acquisition module for collecting signals from the optical detection module, a spatial translation mechanism, a linear translation mechanism, and a dark chamber for installing the optical detection module.

[0038] In this embodiment, the spatial translation of the sampling needle and the magnetic control module relative to the sample carrier, the reagent carr...

Embodiment 2

[0042] A droplet chip nucleic acid analysis system, comprising: an installation platform as a basic installation plane, a sample carrier platform for carrying nucleic acid analysis samples, a reagent carrier platform for nucleic acid analysis reagents, a chip carrier platform for open chips, and a sample carrier platform , Reagent carrier, sampling needle for translation between chip carriers, temperature control module for temperature control of the chip, magnetic control module for magnetic bead transfer of the contents in the chip, nucleic acid analysis for the contents of the chip An optical detection module, a signal acquisition module for collecting signals from the optical detection module, a plane translation mechanism, a rotating lifting arm, and a dark chamber for installing the optical detection module.

[0043] In this embodiment, the spatial position change of the sampling needle relative to the sample carrier, the reagent carrier, and the chip carrier is realized ...

Embodiment 3

[0047] This embodiment adopts the detection and analysis of hepatitis B virus DNA based on fluorescent quantitative PCR on the micro-pit array chip realized by the analysis system of the first embodiment.

[0048] The experiment uses a glass chip with a size of 10×10cm. The chip has a set of 4×12 microwell arrays, and the volume of each microwell is 50 μL. The surface of the chip is treated with a hydrophobic and oleophobic coating.

[0049] Specific steps are as follows:

[0050] (1) The sampling needle sequentially absorbs 10 μL of mineral oil, 5 μL of magnetic bead suspension and 10 μL of sample lysate, repeats the operation 12 times, and loads them in sequence in each microwell in the first column.

[0051] (2) The sampling needle absorbs 20 μL of mineral oil and 10 μL of washing solution in sequence, and repeats the operation 12 times, and loads them in sequence in each microwell in the second column.

[0052] (3) The sampling needle absorbs 20 μL of mineral oil and 10...

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Abstract

The invention relates to a droplet chip nucleic acid analysis system. The droplet chip nucleic acid analysis system comprises a mounting platform as a base mounting plane, a sample carrying bench forcarrying a nucleic acid analysis sample, a reagent carrying bench for carrying a nucleic acid analysis reagent, chip carrier carrying benches for carrying an open chip, a sampling needle that translates between the chip carrying benches, a temperature control module that controls the temperature of a chip, a magnetic control module that performs magnetic bead transferring on the contents of the chip, an optical detection module for performing nucleic acid analysis on the contents in the chip, and a signal acquisition module for performing signal acquisition on the optical detection module, Theoptical detection module is used to detect optical signals in the droplets of the nucleic acid analysis chip. The invention also relates to an analysis method of the droplet chip nucleic acid analysis system. The system has the advantages of small volume, low reagent consumption, flexible operation and fast analysis speed, and belongs to a medical device for biological detection.

Description

technical field [0001] The invention relates to a medical device for biological detection, in particular to a droplet chip nucleic acid analysis system and an analysis method thereof. Background technique [0002] Nucleic acid analysis can detect genetic material and has the advantages of fast, accurate and sensitive. Typical nucleic acid analysis steps include: sample lysis, nucleic acid extraction and purification, nucleic acid amplification and detection. With the promotion of fluorescent quantitative PCR, nucleic acid detection has been widely used. Nevertheless, the promotion of nucleic acid analysis technology is still restricted by some factors, such as: 1) The venue is strict, and reagent preparation, nucleic acid extraction and amplification need to be carried out in separate rooms, which greatly limits the use of this technology in conditions of limited medical resources. 2) Off-line analysis is used, which is complicated to operate and has a long analysis period...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/38C12M1/36C12M1/34C12Q1/6844C12Q1/6851C12Q1/70C12Q1/04
CPCC12Q1/6844C12Q1/6851C12Q2565/629C12Q2563/107C12Q2531/113C12Q2531/119
Inventor 刘大渔
Owner 广州市第一人民医院
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