The invention discloses a preparation method of carboxymethyl chitosan film. The method is conducted according to the following steps that 1, carboxymethyl chitosan is taken, and is dissolved in waterto form a 0.1-5wt% carboxymethyl chitosan solution, then appropriate amounts of plasticizer and surfactant are added to make the concentrations of the plasticizer and the surfactant equal to 1-10wt%and 0.1-1wt% respectively, and intense stirring is conducted for 0.5-2 h to obtain a casting solution; 2, an appropriate amount of the casting solution is poured in a film-forming container, at 25-50DEG C, vacuum decompression defoaming is conducted, and then in an oven, drying is conducted to form film; 3, in the film-forming container containing the dry film, excessive solidifying liquid is added for a solidifying bath for a certain time, so that wet carboxymethyl chitosan film is obtained, wherein the solidifying liquid is ethyl alcohol or methyl alcohol or acetone, and the purity of the solidifying liquid is larger than 90%; 4, the obtained wet film is dried to obtain the carboxymethyl chitosan film. The preparation method of the carboxymethyl chitosan film is simple, the raw materials are environmentally friendly, the thickness of the film is controllable, and the prepared carboxymethyl chitosan film is wide in application, and can be applied to the aspects of energy, fresh keeping, medicines and the like.