The invention relates to the field of health-care foods, and provides anti-fatigue instant dark green tea. The tea comprises the following raw materials in parts by weight: 50 to 60 parts of dark green tea, 8 to 10 parts of apricot kernels, 2 to 5 parts of honey, 2 to 5 parts of hawthorn, 5 to 8 parts of chrysanthemum, 5 to 8 parts of dateplum persimmon, 2 to 5 parts of acanthopanax, and 2 to 5 parts of royal jelly. A preparation method of the anti-fatigue instant dark green tea comprises the following steps of: A, weighing dark green tea, chrysanthemum and acanthopanax, and performing microwave treatment; B, selecting high-quality apricot kernels, hawthorn and dateplum persimmon; C, mixing the raw materials in step A and step B, and performing ultramicro treatment to obtain tea powder; and D, adding honey and royal jelly into the tea powder, uniformly stirring, frying the powder by using a food powder frying machine, frying for 50 to 60 minutes for each pot, standing and naturally cooling, and packaging to obtain a finished product of which the moisture content is not more than 5 percent. The dark green tea drink can be drunk directly, tastes fine and smooth, and has the health-care effects of resisting fatigue, reducing blood fat, blood pressure and blood sugar, preventing cardiovascular diseases and delaying senescence.