Preparation method of cordyceps militaris cantaloupe enzyme with beauty treatment effect
A technology of Cordyceps militaris and cantaloupe is applied in the directions of bacteria used in food preparation, yeast-containing food ingredients, functions of food ingredients, etc., and can solve the problems of inability to meet people's normal needs and low enzyme content.
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Embodiment 1
[0021] Step 1: Prepare the yeast seed solution:
[0022] Seed liquid tank expansion medium: YPD liquid medium, its mass percentage is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, the rest is water, put YPD liquid medium into 0.5M 3 In the seed tank, sterilize at 115°C for 30 minutes; under aseptic conditions, dissolve 1.5 kg of dry yeast powder in 3 times aseptic water to form a bacterial liquid, put the bacterial liquid into a serum bottle and Inoculate to 0.5M 3 The seed tank is fed with sterile compressed air at 28°C, the ventilation rate is 0.2 cubic meters per minute, and cultivated for 20 hours. When the bacteria grow to the end of the logarithmic phase, the cultivation is stopped. Repeat the above steps to obtain the required quality of yeast Seed liquid of bacterial liquid;
[0023] Step 2: Prepare lactic acid bacteria seed solution:
[0024] Seed liquid tank expansion medium: malt compound juice enrichment medium, its mass percentage is 10% wort, 0.5% soybean...
Embodiment 2
[0032] Step 1: Prepare the yeast seed solution:
[0033] Seed liquid tank expansion medium: YPD liquid medium, its mass percentage is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, the rest is water, put YPD liquid medium into 0.5M 3 In the seed tank, sterilize under high temperature at 115°C for 50 minutes; under aseptic conditions, dissolve 1.5 kg of dry yeast powder in 3 times aseptic water to form a bacterial liquid, put the bacterial liquid into a serum bottle and Inoculate to 0.5M 3 The seed tank is fed with sterile compressed air at 30°C, the ventilation rate is 0.3 cubic meters per minute, cultivated for 30 hours, and the culture is stopped when the bacteria grow to the end of the logarithmic phase, and the yeast of the required quality can be obtained by repeating the above steps Seed liquid of bacterial liquid;
[0034] Step 2: Prepare lactic acid bacteria seed solution:
[0035] Seed liquid tank expansion medium: malt compound juice enrichment medium, its mas...
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