385 results about "Yeast strain" patented technology

The invention belongs to the technical field of bioengineering, and discloses a recombinant yarrowia lipolytica capable of efficiently and rapidly producing sclareol, which is characterized in that optimized sclareol synthase related coding genes ScLPPS, ScSCS, GGPPS1, GGPPS2, ERG20 and tHMGR coding genes are inserted into a yarrowia lipolytica genome; inserting optimized pentadienol utilization pathway coding genes such as IPK, THIM and IDI coding genes; and inserting an optimized ATP: citrate lyase coding gene ACLY to obtain the ATP: citrate lyase coding gene ACLY. The recombinant yarrowia lipolytica is based on knockout of a yarrowia lipolytica strain responsible for coding a non-homologous recombination gene ku70, so that the homologous recombination capability of the yarrowia lipolytica strain is enhanced, gene integration is realized through the homologous recombination function of the yarrowia lipolytica, the genetic stability of the introduced gene can be greatly improved, and the recombinant yarrowia lipolytica strain has a good application prospect. The construction method of the recombinant yarrowia lipolytica strain is efficient and simple to operate.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention belongs to the technical field of protein engineering and genetic engineering, and particularly relates to recombinant IV-type humanized collagen as well as a preparation method and application thereof. A section of brand new recombinant collagen is designed and obtained through methods such as screening, replacement and splicing, the nucleotide sequence of the recombinant collagen is optimized through pichia pastoris codon preference, then a corresponding genetic engineering yeast strain is constructed and obtained, and the recombinant collagen is successfully prepared through fermentation. Experiments prove that the recombinant IV-type humanized collagen prepared by the invention is large in molecular weight and high in yield, and compared with commercially available recombinant collagen, the recombinant IV-type humanized collagen has better free radical scavenging performance and cell proliferation and migration promoting performance, and has good antioxidant and skin barrier repair functions, so that the recombinant IV-type humanized collagen has good practical application value.

The present invention relates to a genetically engineered strain of Saccharomyces cerevisiae for the synthesis of 7-DHC exocytosis, and a construction method and application thereof. The present invention constructs a recombinant S.cerevisiae strain sc13 that can efficiently transport 7-DHC to the extracellular space. When it is used for 500mL shake flask biphasic fermentation, the total production of 7-DHC reaches 28.189mg / g (secretion amount 11.701mg / g), compared with the control sc1 strain, the total production of 7-DHC increases by 14.54 times, and the total extracellular secretion increases by 13.77 times, wherein the secretion production of extracellular 7-DHC accounts for 41.51%. The recombinant strain constructed by the present invention has stronger exocrine ability and higher yield, provides a guiding idea for the synthesis of 7-DHC and the simplification of the separation and extraction of 7-DHC, and has broad application prospects.

The invention relates to the field of microbial fermentation, in particular to an ethyl carbamate-reducing Caribik Yarrowia spp. And application of the Caribik Yarrowia spp. The invention discloses Meyerozyma caribba P3, the enzyme activity of ethyl carbamate hydrolase of the Meyerozyma caribba P3 is high and can reach 980.40 U, and when the Meyerozyma caribba P3 is applied to yellow wine fermentation, 49.5% of yellow wine EC can be degraded. The yeast strain for degrading the ethyl carbamate in the fermented food has the advantages of being high in fermentation capacity, capable of tolerating low-acid, high-sugar and 12% alcohol environments and the like, and a valuable strain source is provided for degrading the ethyl carbamate in the fermented food through a microbiological method.

The invention belongs to the field of food microorganisms, and particularly relates to a saccharomycopsis fibuligera strain, a microbial inoculum, a fermented product and preparation and application thereof. The saccharomycopsis fibuligera strain provided by the invention is preserved in the China Center for Type Culture Collection on September 25, 2023, and the preservation number of the saccharomycopsis fibuligera strain is CCTCC NO: M 20231788. The saccharomycopsis fibuligera strain provided by the invention can produce amylase, protease, cellulase and other enzymes required by fermentation, the amylase has the activity of pullulanase and raw amylase and can well adapt to the special environmental conditions of fermentation, and the saccharomycopsis fibuligera strain has high environmental tolerance and high aroma-producing capacity; therefore, the quality of fermented products is improved, and the fermentation production investment cost is reduced.

The present invention discloses a yeast engineering bacterium containing a (-)-α-bisabolol synthase mutant and its application. By designing (-)-α-bisabolol synthase, the present invention obtains a mutant with high expression level, co-expresses the mutant with ERG20, highly expresses the key rate-limiting enzymes in the metabolic pathway, deletes the promoter to reduce the side reaction pathway, and adopts a multi-copy strategy to improve the metabolic flux in the (-)-α-bisabolol biosynthesis pathway. The shake-flask fermentation yield of the yeast engineering bacterium constructed by the present invention is close to 70 mg / L, which is about 35 times higher than that of the starting strain, and has broad application prospects in the biological fermentation production of (-)-α-bisabolol.

The present invention discloses a Kluyveromyces marxianus yeast strain, a method for fermenting rose-flavored coffee beans and the coffee beans. The Kluyveromyces marxianus yeast strain is selected as the fermentation strain. By obtaining and preserving microorganisms from sour meat, the green coffee beans are co-fermented with Damascus rose flowers, thereby endowing the coffee beans with a unique rose flavor. The green coffee beans are subjected to high-temperature sterilization and the rose petals are subjected to ultraviolet sterilization treatment; then inoculation is carried out using a fermentation strain with a specific concentration; then multiple fermentations are carried out in a constant temperature and humidity environment to fully integrate the rose aroma and coffee flavor. Through selective fermentation and precise control of the inoculation concentration, it is ensured that the flavor compounds generated during the fermentation process are consistent, achieving a stable flavor performance. The finally obtained rose-flavored coffee has a unique flavor, moderate acidity, good body, balanced sweetness, appropriate roasting degree and uniform particle size. Its moisture content and trace element content are precisely controlled, and the tasting score reaches 81.3 points.

The invention discloses a salt-resistant and acid-resistant yeast fungus capable of efficiently degrading benzene series and application of the yeast fungus, the yeast fungus is Barnettozyma hawaiiensis, and the preservation number is CCTCC (China Center for Type Culture Collection) M 20241391. The yeast strain can keep activity in a high-salinity and acidic environment, obviously reduces the content of organic matters in high-salinity wastewater, and shows efficient degradation capacity on benzene series, especially benzaldehyde, in the high-salinity acidic wastewater. The method is especially suitable for treating high-salt or acidic organic wastewater and refractory industrial organic wastewater containing benzene series such as shale gas flowback fluid. An effective bacterium source is provided for biochemical treatment of high-salt acidic refractory industrial organic wastewater containing benzene series and the like.

The invention relates to the technical field of microorganisms, in particular to saccharomyces cerevisiae CEC NRI16 and application thereof. The invention provides Saccharomyces cerevisiae CEC NRI16, which is used for wine brewing, can significantly improve the variety aroma and the content of ester substances and terpene substances in dry white wine, especially can promote the generation of ethyl acetate, isoamyl acetate, ethyl hexanoate, ethyl caprate and linalool, reduces the generation of isoamyl alcohol, and improves the yield of wine. The grape wine presents richer fragrance of green fruits such as pears and apples and fragrance of flower fragrance such as violet and roses, and the raw and green taste is effectively reduced. The yeast strain is strong in fermentation capacity and excellent in fermentation performance, and has great value in the aspects of maintaining the typicality of grape wine varieties and enhancing flavor characteristics.

The invention relates to the technical field of collagen, and discloses a preparation method of recombinant engineering bacteria for expressing III-type collagen COL17A1, which comprises the following steps: synthesizing an optimized sequence of human III-type collagen, and adding proper restriction enzyme cutting sites at two ends of the sequence. According to the preparation method of the recombinant engineering bacterium for expressing the III-type collagen COL17A1, codon optimization is carried out on a target sequence by adopting a yeast expression system, OptimGeneTM codon optimization is provided according to codon preference of yeast strains such as pichia pastoris, and the yield of recombinant protein is further improved. And a proper vector is selected to realize high-efficiency expression in pichia pastoris. For example, a pGAPZ A-alpha A vector stably expresses recombinant protein in pichia pastoris by using a GAP promoter, is a constitutive gene expression vector, and can maintain a stable expression rate in cells no matter how the environment changes. An inducer does not need to be added, the production process is simplified, the production cost is reduced, and the production efficiency is improved.

The invention relates to the technical field of industrial microorganisms, and particularly discloses a yeast strain and application thereof. The invention provides clavipora lusitaniae YJHT233, which is obtained through space mutation breeding, can tolerate 9% of alcohol, can tolerate an acid environment with the lowest pH value of 2.5, can tolerate a 12% high NaCl system, a 55% high glucose system and a 44 DEG C high temperature environment, and has excellent ester production capacity. The strain is used for brewing rice-flavor liquor or yellow wine, the total content of ester substances in the obtained product is greatly increased, the content of main volatile ester substances such as ethyl acetate, ethyl butyrate, isoamyl acetate, ethyl caprylate and ethyl lactate is remarkably increased, the whole flavor is coordinated and pleasant, and the fruit fragrance is rich and rich in layers.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention provides noodles with a low glycemic index (GI) and a preparation method thereof, and relates to the technical field of food processing. The low-GI noodles provided by the invention are prepared from the following raw materials in parts by mass: 5-10 parts of kale powder, 8-14 parts of bean dreg and wheat bran powder, 75-85 parts of flour and other auxiliary materials, and the bean dreg and wheat bran powder is prepared by mixing a yeast strain in a laboratory with dry bean dregs and dry wheat bran (in a mass ratio of 1: 1), fermenting and then carrying out superfine grinding. The noodles provided by the invention are low-glycemic-index food, have the advantages of low sugar, low fat, low salt, high fiber, moderate hardness, good elasticity and uniform tissue structure, are simple and convenient in processing method and high in product stability, are suitable for serving as staple food to be applied to diet management of diabetes and obesity, are beneficial to improving the blood sugar level of related people, and are suitable for being eaten by people with diabetes and obesity. The overall health condition of citizens is improved.

The invention belongs to the field of microorganisms, and particularly relates to a salt-resistant and acid-resistant rhodotorula sp. Capable of producing ethyl phenylacetate and application of the rhodotorula sp. In order to develop a new path for the source of ethyl phenylacetate, the invention screens, separates and purifies a strain of salt-resistant and acid-resistant Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from thick broad-bean sauce, and the preservation number is CCTCC NO: M 2025465. The invention also discloses a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce and a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce. The strain has the characteristics of high temperature resistance, acid resistance and salt resistance; and when the strain is used for fermentation culture of thick broad-bean sauce, the yield of ethyl phenylacetate can be increased from 104.86 mu g / Kg to 522.11 mu g / Kg. Therefore, when the rhodotorula sp. S9 is applied to the field of food fermentation, not only can the fermentation flavor of foods such as thick broad-bean sauce, soy sauce and bread be improved, but also a novel method for synthesizing ethyl phenylacetate simply, conveniently, environment-friendly and safely can be provided, and the rhodotorula sp. S9 has a wide prospect in the field of food application.

The invention relates to a preparation method of Parasin I protein or Parasin I fermentation liquor. The preparation method comprises the following steps: obtaining a yeast strain capable of secreting the Parasin I protein; inoculating the saccharomycetes strain into a specific liquid culture medium; fermenting the yeast strain and obtaining Parasin I fermentation liquor; the fermentation liquor comprises Parasin I protein; wherein the pH value of the specific culture medium is 6-9, the inoculum size of the saccharomycetes strain in the specific culture medium is 0.005-0.015 OD / mL, and the temperature of the fermentation saccharomycetes is 28-33 DEG C. The antibacterial peptide Parasin I protein or Parasin I fermentation liquor is simple in preparation method and high in yield, the immunity of fishes can be remarkably improved, and the survival rate of the fishes infected by pathogens is increased.

Provided herein, inter alia, are methods for treating, delaying and / or reducing the severity of conditions associated with age-related decline of various physiological pathways, including mitochondrial health and associated physical states, by administration of compositions that can degrade extracellular ATP (eATP), including NTPDase enzymes, nucleotidases such as apyrases and members of the GDA1_CD39 superfamily, phosphatases and ATP degrading enzyme producing yeast strains.

The invention relates to a saccharomycetes strain with high yield of esterifying enzyme and application thereof, the classification name of the saccharomycetes strain is Wickerhamomyces anomalus, the saccharomycetes strain is preserved in China General Microbiological Culture Collection Center on March 21, 2025, the preservation number is CGMCC 33925, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain screened by the invention has the ability of high yield of esterifying enzyme, and can synchronously catalyze synthesis of a plurality of key esters such as ethyl acetate, ethyl hexanoate, ethyl lactate and the like. Through the synergistic effect of polyesters, the flavor level of the liquor body is richer, and the liquor body is close to the composite aroma characteristic of the traditional solid fen-flavor liquor. The strain is naturally adapted to brewing of fen-flavor liquor and intensified application of medium and low temperature yeast, exogenous enzyme dependence is reduced, and the esterification capacity of yeast is improved.

The invention provides a saccharomycopsis fibuligera fermentation broth, a preparation method and application thereof, and belongs to the technical field of microbial fermentation, the saccharomycopsis fibuligera fermentation broth is prepared by fermentation of saccharomycopsis fibuligera, the saccharomycopsis fibuligera is one of saccharomycopsis fibuligera strains CICC 33377, CICC 1803, CCTCC CB 20081663 and CCTCC DY 20081403, a culture medium for fermentation contains rice bran powder, and the culture medium is a culture medium for fermentation of the saccharomycopsis fibuligera. The saccharomycopsis fibuligera fermentation liquor contains gamma-oryzanol. Through the metabolic activity of saccharomycopsis fibuligera, beneficial components, especially gamma-oryzanol, in the rice bran are extracted at a high level, and the economic value of the rice bran is remarkably improved.

The invention provides auxotroph hansenula polymorpha as well as a construction method and application thereof. The method comprises the following steps: constructing a CRISPR gene editing plasmid of single-plasmid double-point editing yeast, introducing the plasmid into a hansenula polymorpha strain, and constructing the auxotroph hansenula polymorpha by replacing an sgRNA sequence in the plasmid, so as to obtain the auxotroph hansenula polymorpha. The auxotroph hansenula polymorpha keeps a high growth rate of an original strain, genetically engineered bacteria capable of efficiently producing exogenous target proteins can be rapidly screened out through auxotroph screening, expression products can be selectively accumulated in cells and can also be selectively secreted into fermentation liquor, and the yield of the heterotroph hansenula polymorpha is improved. The expressed recombinant protein has high biological safety and can be applied to multiple fields of medicine, medical beauty, instruments, food, animal husbandry and the like.

Provided are a brewer's yeast having low ethanol synthesis and high acetyl-CoA synthesis, and a use thereof, belonging to the technical field of biology. In order to reduce the accumulation of ethanol, the main byproduct of brewer's yeast, and increase synthesis of acetyl-CoA, MTH1, MED2 and HXT2 transcription factors are modified, an optimal transcription factor combination for reducing ethanol synthesis is obtained by screening, and then heterologous acetyl-CoA synthesis pathways of different synthesis pathways integrated in the brewer's yeast genome are compared, and a recombinant brewer's yeast strain is provided. The strain can grow in a sterile culture medium using glucose as a carbon source, creating a platform for modifying brewer's yeast by metabolic engineering, to synthesize high-value compounds; in addition, the construction method is simple and easy to use, and has good application prospects.

Provided herein are methods and materials for using organisms (e.g., engineered yeast strain-derived) to generate lipid compositions which can modulate cellular aging, extend lifespan, and / or modulate gut homeostasis in a subject. The lipid compositions contain at least twelve of the lipids selected from a phosphatidylethanolamine (PE); a lysophosphatidylethanolamine (LPE); a diacylglycerol (DG); a monoglyceride (MG); an acyl carnitine (AcCa); a phosphatidic acid (PA); a phosphatidylglycerol (PG); a lysophosphatidylcholine (LPC); a triacylglycerol (TG); a cholesterol ester (ChE); a wax ester (WE); a phosphatidylserine (PS); a phosphatidylcholine (PC); a phosphatidylinositol (PI); a ceramide (Cer); and a lysophosphatidylserine (LPS).

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof in preparation of ethyl palmitate, propyl linoleate or ethyl oleate. According to the present invention, the Wickerhamomyces anomalus is provided, the Wickerhamomyces anomalus is the Wickerhamomyces anomalus Lz5, the classification name of the Wickerhamomyces anomalus is Wickerhamomyces anomalus, the Wickerhamomyces anomalus is preserved in the China General Microbiological Culture Collection Center on June 19, 2025, and the preservation number of the Wickerhamomyces anomalus is CGMCC No. 34954; the address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing; when the strain is used for fermenting sugarcane juice, ethyl palmitate, propyl linoleate and ethyl oleate can be prepared. In the culture medium after fermentation is finished, the content of ethyl palmitate can reach 13.68 mg / L. After subsequent extraction, the method can be used for industrial production.

The invention provides yeast HAU2501 and application thereof in preparation of fermented vegetables, and belongs to the technical field of fermentation microorganisms. The invention discloses saccharomycetes HAU2501, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 21, 2025, and the preservation number is CGMCC No.35320. The saccharomycetes HAU2501 has the advantages that the saccharomycetes HAU2501 can be used for preparing the saccharomycetes HAU2501; the saccharomycetes HAU2501 can be used as an excellent aroma-producing leavening agent, can effectively improve the flavor quality of the fermented vegetables through synergistic fermentation with lactic acid bacteria, and provides a theoretical basis and a technical reference for flavor regulation and control of traditional fermented vegetables and development of functional leavening agents.

This invention relates to a highly stress-tolerant cyst-forming yeast and its application in aged vinegar production, belonging to the field of vinegar brewing technology. To screen for ester-producing yeast strains with high stress tolerance and apply them to the aged vinegar brewing process, this invention isolated a yeast strain ZLCY-93 from the fermentation mash and vinegar mash of Shanxi aged vinegar. This strain exhibits high enzyme production, high ester production capacity, stronger adaptability to high temperature, high acidity, high osmotic pressure, and high concentration of ethanol during fermentation, good fermentation performance, and outstanding aroma production. Morphological and molecular biological analysis identified this yeast strain as a cyst-forming yeast. This invention combines yeast ZLCY-93 with practical experience in aged vinegar production to create bran koji, which, when applied to aged vinegar production, can shorten the fermentation cycle, improve raw material utilization, promote flavor compound formation, and enhance product quality.

The invention is applicable to the technical field of microorganisms, and provides an aroma-producing yeast and application thereof in beer fermentation, the aroma-producing yeast is Meijie yeast, and the preservation number of the aroma-producing yeast is CGMCC (China General Microbiological Culture Collection Center) NO.33897. The method comprises the following steps: separating an aroma-producing yeast strain from a natural environment, purifying the aroma-producing yeast strain, screening the purified strain, selecting dominant aroma-producing yeast with better tolerance, carrying out a next experiment, determining a growth curve of the dominant aroma-producing yeast, identifying the strain, observing the form of the strain, and carrying out fermentation performance evaluation. The bacterial strain is good in stress resistance and good in growth ability, and when the bacterial strain is applied to beer fermentation through mixed fermentation, the bacterial strain has advantages in multiple sensory dimensions such as foam, flavor coordination, various aromas and killing taste compared with commercial beer obtained through single-bacterium fermentation. And better drinking experience can be provided for consumers.

The invention relates to the technical field of biology, in particular to a recombinant strain for synthesizing vanillin and application of the recombinant strain. According to the invention, a vanillin non-degradable yeast strain is taken as a chassis, a new way for efficiently synthesizing vanillin by taking glucose as a precursor is designed and constructed, chorismic acid is taken as a key intermediate, chorismic acid lyase UbiC is introduced to convert the chorismic acid into p-hydroxybenzoic acid, and then vanillin is gradually synthesized through carboxylic acid reduction, hydroxylation and methylation; and more efficient de novo synthesis of vanillin and glucose vanillin is realized subsequently through strategies such as optimization of integration sites and removal of glycosylation in-situ products. The constructed engineering strain has the capacity of efficiently converting glucose to synthesize vanillin, the highest value reported at present can be obtained through fermentation in a shake flask, higher yield is obtained through further optimization of a bioreactor, and important reference value is provided for subsequent production and application.

The invention provides pure red beer and a malt compounding method thereof, and belongs to the technical field of beer brewing. The pure red beer provided by the invention consists of basic malt and special malt as raw materials, and is obtained by obtaining saccharified wort through a saccharification process, oxygenating and adding a yeast strain for fermentation, the color values of the obtained pure red wine liquid are L * 75-83, a * 32-39 and b * 52-57. The beer has typical wheat fragrance flavor, is pure in color and rich in wheat fragrance flavor, and can further enrich beer categories in the market.

The invention belongs to the technical field of bioengineering, and relates to industrial microorganism breeding, in particular to a high-yield ethyl acetate diploid / tetraploid saccharomyces cerevisiae strain as well as a construction method and application thereof. According to the research, a diploid / tetraploid saccharomyces cerevisiae strain is constructed on the basis of an existing high-yield ethyl acetate haploid yeast strain by utilizing a CRISPR / Cas9 mediated mating type conversion technology. And a series of diploid / tetraploid saccharomyces cerevisiae strains with the acetic acid and lactic acid tolerance and the ethyl acetate yield obviously improved compared with the starting strain are obtained. According to the series of strains, ethyl acetate is high in yield while the excellent alcoholic fermentation characteristic is kept in the fermentation process, white spirit fermented by the series of strains is more prominent in ester fragrance and grain fragrance, weaker in foreign flavor and better in taste on the basis of guaranteeing the original style, a new thought is provided for the breeding mode of yeast strains, and the method is worthy of popularization and application. And a reference is provided for production optimization of ethyl acetate in a Baijiu fermentation process.

The present invention provides an engineered strain of yeast having mitochondrion-positioned reductive TCA pathway and efficiently producing succinic acid, a construction method therefor and use thereof, and belongs to the technical field of microorganisms and fermentation engineering. According to the present invention, the enzymes related to the biosynthesis pathway of succinic acid in yeast cells are overexpressed and positioned to the mitochondrial matrix to facilitate the functional expression of the reductive TCA pathway in eukaryotic microorganisms. The strain can utilize common carbon and nitrogen sources, and after fed-batch fermentation culture, the succinic acid yield reaches 50.1 g / L, and the conversion rate reaches 0.75 g / g of glucose. As an excellent strain for efficiently producing succinic acid, the strain has good application prospects in the fields of food, chemical engineering, degradable materials, and the like.