330 results about "Yeast strain" patented technology

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The present invention relates to a genetically engineered strain of Saccharomyces cerevisiae for the synthesis of 7-DHC exocytosis, and a construction method and application thereof. The present invention constructs a recombinant S.cerevisiae strain sc13 that can efficiently transport 7-DHC to the extracellular space. When it is used for 500mL shake flask biphasic fermentation, the total production of 7-DHC reaches 28.189mg / g (secretion amount 11.701mg / g), compared with the control sc1 strain, the total production of 7-DHC increases by 14.54 times, and the total extracellular secretion increases by 13.77 times, wherein the secretion production of extracellular 7-DHC accounts for 41.51%. The recombinant strain constructed by the present invention has stronger exocrine ability and higher yield, provides a guiding idea for the synthesis of 7-DHC and the simplification of the separation and extraction of 7-DHC, and has broad application prospects.

The invention belongs to the field of food microorganisms, and particularly relates to a saccharomycopsis fibuligera strain, a microbial inoculum, a fermented product and preparation and application thereof. The saccharomycopsis fibuligera strain provided by the invention is preserved in the China Center for Type Culture Collection on September 25, 2023, and the preservation number of the saccharomycopsis fibuligera strain is CCTCC NO: M 20231788. The saccharomycopsis fibuligera strain provided by the invention can produce amylase, protease, cellulase and other enzymes required by fermentation, the amylase has the activity of pullulanase and raw amylase and can well adapt to the special environmental conditions of fermentation, and the saccharomycopsis fibuligera strain has high environmental tolerance and high aroma-producing capacity; therefore, the quality of fermented products is improved, and the fermentation production investment cost is reduced.

The present invention discloses a yeast engineering bacterium containing a (-)-α-bisabolol synthase mutant and its application. By designing (-)-α-bisabolol synthase, the present invention obtains a mutant with high expression level, co-expresses the mutant with ERG20, highly expresses the key rate-limiting enzymes in the metabolic pathway, deletes the promoter to reduce the side reaction pathway, and adopts a multi-copy strategy to improve the metabolic flux in the (-)-α-bisabolol biosynthesis pathway. The shake-flask fermentation yield of the yeast engineering bacterium constructed by the present invention is close to 70 mg / L, which is about 35 times higher than that of the starting strain, and has broad application prospects in the biological fermentation production of (-)-α-bisabolol.

The present invention discloses a Kluyveromyces marxianus yeast strain, a method for fermenting rose-flavored coffee beans and the coffee beans. The Kluyveromyces marxianus yeast strain is selected as the fermentation strain. By obtaining and preserving microorganisms from sour meat, the green coffee beans are co-fermented with Damascus rose flowers, thereby endowing the coffee beans with a unique rose flavor. The green coffee beans are subjected to high-temperature sterilization and the rose petals are subjected to ultraviolet sterilization treatment; then inoculation is carried out using a fermentation strain with a specific concentration; then multiple fermentations are carried out in a constant temperature and humidity environment to fully integrate the rose aroma and coffee flavor. Through selective fermentation and precise control of the inoculation concentration, it is ensured that the flavor compounds generated during the fermentation process are consistent, achieving a stable flavor performance. The finally obtained rose-flavored coffee has a unique flavor, moderate acidity, good body, balanced sweetness, appropriate roasting degree and uniform particle size. Its moisture content and trace element content are precisely controlled, and the tasting score reaches 81.3 points.

The invention relates to the technical field of microorganisms, in particular to saccharomyces cerevisiae CEC NRI16 and application thereof. The invention provides Saccharomyces cerevisiae CEC NRI16, which is used for wine brewing, can significantly improve the variety aroma and the content of ester substances and terpene substances in dry white wine, especially can promote the generation of ethyl acetate, isoamyl acetate, ethyl hexanoate, ethyl caprate and linalool, reduces the generation of isoamyl alcohol, and improves the yield of wine. The grape wine presents richer fragrance of green fruits such as pears and apples and fragrance of flower fragrance such as violet and roses, and the raw and green taste is effectively reduced. The yeast strain is strong in fermentation capacity and excellent in fermentation performance, and has great value in the aspects of maintaining the typicality of grape wine varieties and enhancing flavor characteristics.

The invention discloses saccharomyces cerevisiae recombinant bacteria for efficiently producing terpenoids as well as a construction method and application of the saccharomyces cerevisiae recombinant bacteria. According to the construction method, saccharomyces cerevisiae rich in terpenoid precursor farnesyl pyrophosphate is taken as a starting strain, the GAL80 protein level in a galactose induction system is regulated and controlled by introducing an N-Degron strategy, and meanwhile, a glucose concentration response type promoter HXT1 is introduced to effectively regulate and control the expression of GAL80 from the transcriptional level; the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids can be obtained. The strategy is applied to a saccharomyces cerevisiae engineering strain of beta-elemene and patchouli alcohol, and a remarkable effect is achieved. And in the high-density fermentation process of the saccharomyces cerevisiae, the yield of the beta-elemene is obviously increased and reaches 4818.6 mg / L. The invention provides a solid research basis for sustainable biosynthesis of terpenoids, and has a wide application prospect.

The invention provides noodles with a low glycemic index (GI) and a preparation method thereof, and relates to the technical field of food processing. The low-GI noodles provided by the invention are prepared from the following raw materials in parts by mass: 5-10 parts of kale powder, 8-14 parts of bean dreg and wheat bran powder, 75-85 parts of flour and other auxiliary materials, and the bean dreg and wheat bran powder is prepared by mixing a yeast strain in a laboratory with dry bean dregs and dry wheat bran (in a mass ratio of 1: 1), fermenting and then carrying out superfine grinding. The noodles provided by the invention are low-glycemic-index food, have the advantages of low sugar, low fat, low salt, high fiber, moderate hardness, good elasticity and uniform tissue structure, are simple and convenient in processing method and high in product stability, are suitable for serving as staple food to be applied to diet management of diabetes and obesity, are beneficial to improving the blood sugar level of related people, and are suitable for being eaten by people with diabetes and obesity. The overall health condition of citizens is improved.

The invention belongs to the field of microorganisms, and particularly relates to a salt-resistant and acid-resistant rhodotorula sp. Capable of producing ethyl phenylacetate and application of the rhodotorula sp. In order to develop a new path for the source of ethyl phenylacetate, the invention screens, separates and purifies a strain of salt-resistant and acid-resistant Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from thick broad-bean sauce, and the preservation number is CCTCC NO: M 2025465. The invention also discloses a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce and a preparation method of the Rhodotorula daliensis S9 capable of producing ethyl phenylacetate from the thick broad-bean sauce. The strain has the characteristics of high temperature resistance, acid resistance and salt resistance; and when the strain is used for fermentation culture of thick broad-bean sauce, the yield of ethyl phenylacetate can be increased from 104.86 mu g / Kg to 522.11 mu g / Kg. Therefore, when the rhodotorula sp. S9 is applied to the field of food fermentation, not only can the fermentation flavor of foods such as thick broad-bean sauce, soy sauce and bread be improved, but also a novel method for synthesizing ethyl phenylacetate simply, conveniently, environment-friendly and safely can be provided, and the rhodotorula sp. S9 has a wide prospect in the field of food application.

The invention relates to a preparation method of Parasin I protein or Parasin I fermentation liquor. The preparation method comprises the following steps: obtaining a yeast strain capable of secreting the Parasin I protein; inoculating the saccharomycetes strain into a specific liquid culture medium; fermenting the yeast strain and obtaining Parasin I fermentation liquor; the fermentation liquor comprises Parasin I protein; wherein the pH value of the specific culture medium is 6-9, the inoculum size of the saccharomycetes strain in the specific culture medium is 0.005-0.015 OD / mL, and the temperature of the fermentation saccharomycetes is 28-33 DEG C. The antibacterial peptide Parasin I protein or Parasin I fermentation liquor is simple in preparation method and high in yield, the immunity of fishes can be remarkably improved, and the survival rate of the fishes infected by pathogens is increased.

The invention relates to a saccharomycetes strain with high yield of esterifying enzyme and application thereof, the classification name of the saccharomycetes strain is Wickerhamomyces anomalus, the saccharomycetes strain is preserved in China General Microbiological Culture Collection Center on March 21, 2025, the preservation number is CGMCC 33925, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain screened by the invention has the ability of high yield of esterifying enzyme, and can synchronously catalyze synthesis of a plurality of key esters such as ethyl acetate, ethyl hexanoate, ethyl lactate and the like. Through the synergistic effect of polyesters, the flavor level of the liquor body is richer, and the liquor body is close to the composite aroma characteristic of the traditional solid fen-flavor liquor. The strain is naturally adapted to brewing of fen-flavor liquor and intensified application of medium and low temperature yeast, exogenous enzyme dependence is reduced, and the esterification capacity of yeast is improved.

The invention provides auxotroph hansenula polymorpha as well as a construction method and application thereof. The method comprises the following steps: constructing a CRISPR gene editing plasmid of single-plasmid double-point editing yeast, introducing the plasmid into a hansenula polymorpha strain, and constructing the auxotroph hansenula polymorpha by replacing an sgRNA sequence in the plasmid, so as to obtain the auxotroph hansenula polymorpha. The auxotroph hansenula polymorpha keeps a high growth rate of an original strain, genetically engineered bacteria capable of efficiently producing exogenous target proteins can be rapidly screened out through auxotroph screening, expression products can be selectively accumulated in cells and can also be selectively secreted into fermentation liquor, and the yield of the heterotroph hansenula polymorpha is improved. The expressed recombinant protein has high biological safety and can be applied to multiple fields of medicine, medical beauty, instruments, food, animal husbandry and the like.

Provided herein are methods and materials for using organisms (e.g., engineered yeast strain-derived) to generate lipid compositions which can modulate cellular aging, extend lifespan, and / or modulate gut homeostasis in a subject. The lipid compositions contain at least twelve of the lipids selected from a phosphatidylethanolamine (PE); a lysophosphatidylethanolamine (LPE); a diacylglycerol (DG); a monoglyceride (MG); an acyl carnitine (AcCa); a phosphatidic acid (PA); a phosphatidylglycerol (PG); a lysophosphatidylcholine (LPC); a triacylglycerol (TG); a cholesterol ester (ChE); a wax ester (WE); a phosphatidylserine (PS); a phosphatidylcholine (PC); a phosphatidylinositol (PI); a ceramide (Cer); and a lysophosphatidylserine (LPS).

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof in preparation of ethyl palmitate, propyl linoleate or ethyl oleate. According to the present invention, the Wickerhamomyces anomalus is provided, the Wickerhamomyces anomalus is the Wickerhamomyces anomalus Lz5, the classification name of the Wickerhamomyces anomalus is Wickerhamomyces anomalus, the Wickerhamomyces anomalus is preserved in the China General Microbiological Culture Collection Center on June 19, 2025, and the preservation number of the Wickerhamomyces anomalus is CGMCC No. 34954; the address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing; when the strain is used for fermenting sugarcane juice, ethyl palmitate, propyl linoleate and ethyl oleate can be prepared. In the culture medium after fermentation is finished, the content of ethyl palmitate can reach 13.68 mg / L. After subsequent extraction, the method can be used for industrial production.

The invention provides yeast HAU2501 and application thereof in preparation of fermented vegetables, and belongs to the technical field of fermentation microorganisms. The invention discloses saccharomycetes HAU2501, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 21, 2025, and the preservation number is CGMCC No.35320. The saccharomycetes HAU2501 has the advantages that the saccharomycetes HAU2501 can be used for preparing the saccharomycetes HAU2501; the saccharomycetes HAU2501 can be used as an excellent aroma-producing leavening agent, can effectively improve the flavor quality of the fermented vegetables through synergistic fermentation with lactic acid bacteria, and provides a theoretical basis and a technical reference for flavor regulation and control of traditional fermented vegetables and development of functional leavening agents.

This invention relates to a highly stress-tolerant cyst-forming yeast and its application in aged vinegar production, belonging to the field of vinegar brewing technology. To screen for ester-producing yeast strains with high stress tolerance and apply them to the aged vinegar brewing process, this invention isolated a yeast strain ZLCY-93 from the fermentation mash and vinegar mash of Shanxi aged vinegar. This strain exhibits high enzyme production, high ester production capacity, stronger adaptability to high temperature, high acidity, high osmotic pressure, and high concentration of ethanol during fermentation, good fermentation performance, and outstanding aroma production. Morphological and molecular biological analysis identified this yeast strain as a cyst-forming yeast. This invention combines yeast ZLCY-93 with practical experience in aged vinegar production to create bran koji, which, when applied to aged vinegar production, can shorten the fermentation cycle, improve raw material utilization, promote flavor compound formation, and enhance product quality.

The invention is applicable to the technical field of microorganisms, and provides an aroma-producing yeast and application thereof in beer fermentation, the aroma-producing yeast is Meijie yeast, and the preservation number of the aroma-producing yeast is CGMCC (China General Microbiological Culture Collection Center) NO.33897. The method comprises the following steps: separating an aroma-producing yeast strain from a natural environment, purifying the aroma-producing yeast strain, screening the purified strain, selecting dominant aroma-producing yeast with better tolerance, carrying out a next experiment, determining a growth curve of the dominant aroma-producing yeast, identifying the strain, observing the form of the strain, and carrying out fermentation performance evaluation. The bacterial strain is good in stress resistance and good in growth ability, and when the bacterial strain is applied to beer fermentation through mixed fermentation, the bacterial strain has advantages in multiple sensory dimensions such as foam, flavor coordination, various aromas and killing taste compared with commercial beer obtained through single-bacterium fermentation. And better drinking experience can be provided for consumers.

The invention belongs to the technical field of bioengineering, and relates to industrial microorganism breeding, in particular to a high-yield ethyl acetate diploid / tetraploid saccharomyces cerevisiae strain as well as a construction method and application thereof. According to the research, a diploid / tetraploid saccharomyces cerevisiae strain is constructed on the basis of an existing high-yield ethyl acetate haploid yeast strain by utilizing a CRISPR / Cas9 mediated mating type conversion technology. And a series of diploid / tetraploid saccharomyces cerevisiae strains with the acetic acid and lactic acid tolerance and the ethyl acetate yield obviously improved compared with the starting strain are obtained. According to the series of strains, ethyl acetate is high in yield while the excellent alcoholic fermentation characteristic is kept in the fermentation process, white spirit fermented by the series of strains is more prominent in ester fragrance and grain fragrance, weaker in foreign flavor and better in taste on the basis of guaranteeing the original style, a new thought is provided for the breeding mode of yeast strains, and the method is worthy of popularization and application. And a reference is provided for production optimization of ethyl acetate in a Baijiu fermentation process.

The present invention provides an engineered strain of yeast having mitochondrion-positioned reductive TCA pathway and efficiently producing succinic acid, a construction method therefor and use thereof, and belongs to the technical field of microorganisms and fermentation engineering. According to the present invention, the enzymes related to the biosynthesis pathway of succinic acid in yeast cells are overexpressed and positioned to the mitochondrial matrix to facilitate the functional expression of the reductive TCA pathway in eukaryotic microorganisms. The strain can utilize common carbon and nitrogen sources, and after fed-batch fermentation culture, the succinic acid yield reaches 50.1 g / L, and the conversion rate reaches 0.75 g / g of glucose. As an excellent strain for efficiently producing succinic acid, the strain has good application prospects in the fields of food, chemical engineering, degradable materials, and the like.

The invention relates to the technical field of biology, in particular to engineered yeast for high-yield citronellol as well as a preparation method and application of the engineered yeast. According to the invention, genetically engineered bacteria for high yield of citronellol are constructed by means of genetic engineering, SyBESc081300004 is taken as an original strain, Erg20ww, Erg8, tCrGES and CrIS are expressed in a peroxisome region in a room manner, and phosphopentose pathway genes TAL1 and TKL1 and endogenous transporter protein PDR1 are over-expressed, so that the genetically engineered bacteria are obtained; experimental results show that the shake flask fermentation yield of the genetically engineered bacterium reaches 3.38 g / L, the yield of a 100L fermentation tank reaches 10.556 g / L, and the yield of the genetically engineered bacterium is obviously higher than that of other existing citronellol production engineered bacterium.

The invention relates to the technical field of beer production, in particular to a wort oxygenating device, a wort oxygenating method, a saccharification automatic control system of the wort oxygenating device, a yeast strain liquid adding unit, a cold wort entering unit and a fermentation oxygenating unit. And the yeast strain liquid adding unit is communicated with the cold wort entering unit. The fermentation oxygenation unit is communicated with the cold wort inlet unit, and the fermentation oxygenation unit comprises an oxygen communicating pipeline, an oxygen adjusting mechanism and a detection mechanism. The detection mechanism can detect the flow speed of the wort and send a signal to the oxygen adjusting mechanism, and the oxygen adjusting mechanism can receive the signal of the detection mechanism and conduct oxygenation according to the flow speed of the wort of different boiler times. A wort oxygenating and yeast adding automatic control method is integrated into a saccharification automatic control system, so that a complete saccharification automatic control system is formed, and the wort oxygenating and yeast adding section can be automatically controlled. Oxygen filling and yeast adding amount are automatically adjusted in real time according to the wort tank entering flow speed, and it is ensured that the flavor index of the product is stable and meets the enterprise standard.

A yeast cultivation medium and method of yeast cultivation are provided. The yeast cultivation medium can be an aqueous solution including molasses, rice water, and at least one hydrolytic enzyme, which can be a hydrolase. The yeast cultivation method can include adding a yeast strain to the yeast cultivation medium, heating the yeast-containing cultivation medium, and extracting the yeast from the cultivation medium.

The invention relates to the technical field of biology, in particular to yeast hydrolysate and application thereof. According to the method, enzymes are reasonably screened, the obtained compound enzyme can conduct enzymolysis on yeast strains more effectively, so that hydrolysate rich in free-state lipid, amino acid, polysaccharide and other substances is prepared, the hydrolysate is more beneficial to growth and utilization of yeast, the health state of active yeast cell membranes is improved, and yeast fermentation is promoted.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V based on multi-compartment multi-metabolic pathways and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The preparation method comprises the following steps: anchoring an MVA pathway and a squalene synthesis pathway on the surface of a peroxisome membrane, and enabling mogrol synthetase and epoxy squalene cyclization enzyme ERG1 to form a linear polywheat complex through polypeptide interaction; then, through overexpression of MVA pathway key enzyme and introduction of an isopentenol utilization pathway, supply of precursor isopentenyl pyrophosphate and dimethyl allyl pyrophosphate in cytoplasm is increased, and squalene epoxidase ERG1 and mogrol synthetase are anchored in transmembrane ABC efflux protein PDR11 through polypeptide interaction; then anchoring the mogrol synthetase in the lipid droplet subcells; finally, the glucosyltransferase is displayed on the surface of the yeast cell wall through an alpha-lectin system, so that extracellular mogrol is converted into MG-V. The engineering bacterium improves the yield of mogroside V synthesized from the beginning in microorganisms, and has great industrial application value.

The invention discloses a Kluyveromyces marxianus strain and an application of the Kluyveromyces marxianus strain, and belongs to the field of synthetic biology and microorganism manufacturing. The strain has the characteristics of efficiently utilizing xylose and growing under a low-oxygen condition, and by taking the strain as a fermentation strain, the protein content of fermented straw powder is remarkably improved through the synergistic effect of the strain and cellulase under a straw powder high-solid fermentation condition. According to the yeast strain and the high-solid fermentation process thereof disclosed by the invention, the protein content of straw powder can be greatly increased, and an effective solution is provided for high-valued reutilization of straw agricultural wastes.

The application discloses a strain of yeast for efficiently transforming ginsenosides and polysaccharides and application thereof, and belongs to the technical field of functional component transformation. According to the total ginsenoside and polysaccharide content in ginseng, a strain of yeast for efficiently transforming ginsenosides and polysaccharides is screened by taking a comprehensive score as an index, and is identified as Zygosaccharomyces bisporus by morphology, physiology and biochemistry and molecular biology, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2020912. After transformation, the total ginsenoside content in American ginseng and ginseng is respectively increased by 67.81% and 107.03%, and the polysaccharide content is respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity of the total ginsenoside extract and polysaccharide extract of the American ginseng and ginseng after transformation are all improved. The yeast screened by the application has a wide application prospect.

This invention aims to create a spatially isolated local reaction microenvironment for Rubisco by introducing a complete β-carboxysome microcompartment system. This design goal avoids oxygen inhibition, laying the foundation for ultimately achieving efficient and specific CO2 fixation. It provides a novel approach to overcome the fundamental problem of low efficiency of carbon-fixing enzymes in cytosols due to intense oxygen competition. By introducing the complete carboxysome system as a module, engineered yeast strains with high carboxylase activity and significantly improved ethanol production were obtained, providing a solid foundation for building next-generation yeast cell factories that produce high-yield ethanol using CO2 as a raw material.

The present disclosure relates to the field of bioengineered yeast strains as therapeutic platforms. In particular, engineered strains of yeast brassicae comprising a nucleic acid encoding a modified 3-isopropylmalate dehydrogenase (Leu2) and a therapeutic protein, and methods for treating or preventing a disease or condition in a subject in need thereof.