151 results about "Yeast strain" patented technology

The invention provides yeast HAU2501 and application thereof in preparation of fermented vegetables, and belongs to the technical field of fermentation microorganisms. The invention discloses saccharomycetes HAU2501, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 21, 2025, and the preservation number is CGMCC No.35320. The saccharomycetes HAU2501 has the advantages that the saccharomycetes HAU2501 can be used for preparing the saccharomycetes HAU2501; the saccharomycetes HAU2501 can be used as an excellent aroma-producing leavening agent, can effectively improve the flavor quality of the fermented vegetables through synergistic fermentation with lactic acid bacteria, and provides a theoretical basis and a technical reference for flavor regulation and control of traditional fermented vegetables and development of functional leavening agents.

A yeast cultivation medium and method of yeast cultivation are provided. The yeast cultivation medium can be an aqueous solution including molasses, rice water, and at least one hydrolytic enzyme, which can be a hydrolase. The yeast cultivation method can include adding a yeast strain to the yeast cultivation medium, heating the yeast-containing cultivation medium, and extracting the yeast from the cultivation medium.

The invention relates to the technical field of biology, in particular to yeast hydrolysate and application thereof. According to the method, enzymes are reasonably screened, the obtained compound enzyme can conduct enzymolysis on yeast strains more effectively, so that hydrolysate rich in free-state lipid, amino acid, polysaccharide and other substances is prepared, the hydrolysate is more beneficial to growth and utilization of yeast, the health state of active yeast cell membranes is improved, and yeast fermentation is promoted.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention discloses a Kluyveromyces marxianus strain and an application of the Kluyveromyces marxianus strain, and belongs to the field of synthetic biology and microorganism manufacturing. The strain has the characteristics of efficiently utilizing xylose and growing under a low-oxygen condition, and by taking the strain as a fermentation strain, the protein content of fermented straw powder is remarkably improved through the synergistic effect of the strain and cellulase under a straw powder high-solid fermentation condition. According to the yeast strain and the high-solid fermentation process thereof disclosed by the invention, the protein content of straw powder can be greatly increased, and an effective solution is provided for high-valued reutilization of straw agricultural wastes.

The application discloses a strain of yeast for efficiently transforming ginsenosides and polysaccharides and application thereof, and belongs to the technical field of functional component transformation. According to the total ginsenoside and polysaccharide content in ginseng, a strain of yeast for efficiently transforming ginsenosides and polysaccharides is screened by taking a comprehensive score as an index, and is identified as Zygosaccharomyces bisporus by morphology, physiology and biochemistry and molecular biology, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2020912. After transformation, the total ginsenoside content in American ginseng and ginseng is respectively increased by 67.81% and 107.03%, and the polysaccharide content is respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity of the total ginsenoside extract and polysaccharide extract of the American ginseng and ginseng after transformation are all improved. The yeast screened by the application has a wide application prospect.

This invention aims to create a spatially isolated local reaction microenvironment for Rubisco by introducing a complete β-carboxysome microcompartment system. This design goal avoids oxygen inhibition, laying the foundation for ultimately achieving efficient and specific CO2 fixation. It provides a novel approach to overcome the fundamental problem of low efficiency of carbon-fixing enzymes in cytosols due to intense oxygen competition. By introducing the complete carboxysome system as a module, engineered yeast strains with high carboxylase activity and significantly improved ethanol production were obtained, providing a solid foundation for building next-generation yeast cell factories that produce high-yield ethanol using CO2 as a raw material.

The present disclosure relates to the field of bioengineered yeast strains as therapeutic platforms. In particular, engineered strains of yeast brassicae comprising a nucleic acid encoding a modified 3-isopropylmalate dehydrogenase (Leu2) and a therapeutic protein, and methods for treating or preventing a disease or condition in a subject in need thereof.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The invention relates to the technical field of microorganisms, in particular to a yeast metagen with an anti-allergic effect as well as a preparation method and application of the yeast metagen. According to the invention, the metabolite is an inactivated saccharomyces boulardii strain and a fermentation metabolite thereof, wherein the preservation number of the saccharomyces boulardii strain is CCTCC (China Center for Type Culture Collection) NO: M 20251803. The metagen can be used for remarkably reducing the total IgE (Immunoglobulin E) level of serum and relieving anaphylactic reaction by adjusting the balance of Th1 / Th2, Th17 and Treg immune cells. Therefore, the metabiotics disclosed by the invention have a wide application prospect in preparation of foods, medicines or health-care products for relieving allergic symptoms.

The invention discloses trichosporon arystachys capable of reducing ammonia nitrogen and nitrite and application of the trichosporon arystachys. The saccharomycetes are named as Trichosporonashii JM-1, and the preservation number of the saccharomycetes is GDMCC No: 64448. The invention further discloses a preparation method of the saccharomycetes. The Saccharomyces cerevisiae grows well in a high-concentration ammonia nitrogen and nitrite environment, is high in nitrogen degradation efficiency and good in environmental adaptability, can be used as a functional strain for improving water quality, deodorizing farms, deodorizing garbage yards and the like, is applied to multiple places, reduces nitrogen accumulation, protects the environment, has certain degradation capability on cellulose, and can be used as a functional strain for improving the water quality, deodorizing the farms, deodorizing the garbage yards and the like. The method has great application potential in livestock and poultry aquaculture and environmental protection.

Disclosed herein are yeast strains and derivatives thereof, as well as compositions comprising the yeast strains for use in ethanol production. The disclosure also relates to processes for producing ethanol from biomass using the yeast strains and compositions. In particular, the yeast strains produce less glycerol and more ethanol, and have higher temperature tolerance and faster fermentation rates than strains and products currently used in ethanol production processes.

A yeast biomass composition is formed by culturing a strain of yeast in a substrate such as whey permeate to product a yeast broth, dewatering the broth to produce yeast biomass, and hydrolysing the yeast biomass. The yeast biomass composition finds utility as a feed additive for broiler chickens to reduce the adherence of pathogenic bacteria like Salmonella and E.coli to the gut wall of the chickens, thereby reducing intestinal stress and improving life daily weight gain in the chickens.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

Disclosed are bacterial strain HSCY 2073, isolation and screening thereof, and application thereof in improvement on flavor quality of vinegar. Bacterial strain HSCY 2073 is identified as Lactobacillus sp. and is deposited in China General Microbiological Culture Collection Center, where deposit date is 21 November 2019, and deposit number is CGMCC No. 18982. Bacterial strain HSCY 2073 in the present disclosure is Lactobacillus sp. that has strong acetic acid and ethanol tolerance and excellent effect of producing non-volatile acid, and can produce high contents of 5 aroma substances including acetaldehyde, ethyl acetate, isobutyl acetate, isoamyl acetate and ethyl 3-methylvalerate that have fruit aromas. The lactobacillus can adapt to high-acid, high-alcohol and oligotrophic harsh environments, etc. of vinegar brewing, and has strong ability of high acid tolerance.

An oleaginous yeast strain of the species Trichosporon oleaginosus, deposited on May 28, 2020 at Leibniz-Institut DSMZ, deposit number DSM 33530 can be used in the production of lipids by fermentation and was obtained by mutagenesis and subsequent selection from the oleaginous yeast strain of the species Trichosporon oleaginosus DSM 32508. The strain DSM 33530 is characterized by high thermotolerance, such as to allow the fermentation temperature to be increased compared to conventional processes and still guarantee the vital functions of the microorganism during fermentation itself.

This invention relates to the field of feed, specifically disclosing a method for preparing selenium- and strontium-enriched amino acid chelated feed; including the preparation of selenium / strontium-enriched amino acid fermentation products, the preparation steps of which are as follows: S1: Inoculating activated microalgae and yeast strains into a culture medium supplemented with selenium and strontium compounds, the pH of the culture medium is 6.5-7.5, the culture temperature is 25-35℃, and the culture is carried out under light and aeration conditions for 5-7 days; S2: Collecting selenium / strontium-enriched algal biomass using a flocculant, and ultrasonically treating the selenium / strontium-enriched algal biomass for 10-20 minutes, then adding soybean meal and rapeseed cake to the selenium / strontium-enriched algal biomass and stirring; S3: Adding cellulase, papain, alkaline protease, and pectinase for enzymatic hydrolysis to obtain selenium / strontium-enriched amino acid fermentation products. The selenium- and strontium-enriched amino acid chelated feed and its preparation method of this invention can improve the intestinal health of animals, increase the absorption and utilization rate of nutrients, and enhance animal immunity.

The present application belongs to the field of environmental biotechnology and resource recycling technology, and provides a high-ammonia-nitrogen-tolerant yeast strain and its application in biogas slurry resource treatment. The high-ammonia-nitrogen-tolerant yeast strain is named Torulaspora delbrueckii L12345, which was preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the address is No. 1, Yitian West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 39123. The present application realizes efficient treatment of biogas slurry and simultaneous production of single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-value utilization of biogas slurry.

The invention provides functional saccharomyces cerevisiae and application thereof in fermentation of pueraria polysaccharide, and belongs to the technical field of microbial fermentation. The invention discloses a saccharomyces cerevisiae Y25, which has the physiological characteristics of high ethanol yield, low higher alcohol yield, high ester yield and good tolerance to ethanol, acid and high temperature, can improve the quality of white spirit and enrich and coordinate the flavor of the white spirit, and can be used for fermenting kudzuvine root polysaccharide, so that the obtained fermented polysaccharide is high in yield, strong in oxidation resistance and high in yield. The polysaccharide content is increased by 9.79% compared with commercial Angel yeast, and the DPPH free radical scavenging rate and the hydroxyl free radical scavenging rate are respectively increased by 10.32% and 18.78% compared with the commercial Angel yeast. The saccharomyces cerevisiae Y25 disclosed by the invention has far-reaching social and economic significance on enhanced utilization of functional brewing microbial resources and innovation and upgrading of a traditional brewing process.

Disclosed are genetically engineered Schizosaccharomyces pombe strains capable of fermenting glucose to produce malic acid. The S. pombe strains produce an exogenous malate dehydrogenase (MDH) enzyme and optionally produce an exogenous pyruvate carboxylase (PYC) enzyme or an exogenous phosphoenolpyruvate carboxylase (PPC) enzyme. Additionally, the S. pombe strains may be further engineered to produce a fumarase selectively located in mitochondria and / or have an inactivated mitochondrial citrate transporter, so that the resulting S. pombe strains are capable of producing malic acid as the predominant C4 dicarboxylic acid. Also disclosed are methods of producing malic acid with the S. pombe strains.

Disclosed herein are yeast strains and derivatives thereof, as well as compositions comprising the yeast strains for use in ethanol manufacture. The disclosure also relates to processes for producing ethanol from biomass using the yeast strains and compositions. In particular, one or more of the yeast strains produce higher ethanol, leave less residual fructose, and have a higher temperature tolerance and higher fermentation rate than strains and products currently used in ethanol production processes.

The invention discloses saccharomycetes capable of efficiently converting saponin and polysaccharide and application of the saccharomycetes. Belongs to the technical field of functional component conversion. According to the content of total saponins and polysaccharides in ginseng, a saccharomycete capable of efficiently converting saponins and polysaccharides is screened by taking a comprehensive score as an index, and the saccharomycete is identified as zygosaccharomycete dispora through morphology, physiology, biochemistry and molecular biology and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 2020912. The contents of total saponins in the transformed American ginseng and ginseng are respectively increased by 67.81% and 107.03%, and the contents of polysaccharides are respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, the ABTS antioxidant capacity, the blood sugar reducing capacity and the blood fat reducing capacity of the converted American ginseng and ginseng total saponin extracting solution and the polysaccharide extracting solution are all improved. The saccharomycetes obtained by screening has a wide application prospect.

A yeast strain of Saccharomyces cerevisiae var. Boulardii for the treatment and / or the prevention of infectious diseases of the oral cavity, such as dental caries and periodontal diseases. This strain can be used either alone or in combination with the inactive dry form of a yeast strain of Saccharomyces cerevisiae.

This invention discloses a genetically engineered bacterium for synthesizing carotenoids, its construction method, and its applications, belonging to the field of genetic engineering technology. The genetically engineered bacterium uses a carotenoid-producing yeast strain as the starting material. The gene ROX1, encoding a heme-dependent hypoxia gene repressor, is knocked out, while the expression of genes STB5 (encoding a transcription factor involved in NADPH regeneration), DID2 (encoding a class E protein in the vacuole protein sorting pathway), and VOA1 (encoding an endoplasmic reticulum protein playing a role in the assembly of the V0 region of V-ATPase) is upregulated. This invention promotes carotenoid synthesis and significantly increases carotenoid yield through combined regulation of gene expression outside the carotenoid synthesis pathway, demonstrating promising application prospects.

This invention discloses a strain of Rhodotorula glutinis I7Y2 that promotes cell proliferation in a serum-free environment and its applications. Rhodotorula glutinis ( Rhodotorula mucilaginosa The yeast strain I7Y2 was deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on March 13, 2026, with accession number GDMCC No: 67951. This invention has a good effect on promoting Vero cell proliferation, specifically manifested in: (1) the yeast involved in this invention can produce various active substances such as amino acids, polypeptides, sanshool, and inositol to meet the nutritional needs of cell growth; (2) it can effectively improve the viability and density of Vero cells in the basal culture medium; (3) it can effectively improve the proliferation rate and migration rate of Vero cells in the basal culture medium; and (4) it can effectively reduce the apoptosis rate of Vero cells and the expression of apoptosis-related genes in the basal culture medium. Therefore, Rhodotorula glutinis I7Y2 has great application potential in promoting cell proliferation, especially in serum-free culture products.

The present invention pertains to (Saccharomyces cerevisiae) SPC Y76LT (KCTC 14767BP). The yeast strain (SPC Y76LT) of the present invention exhibits excellent fermentation efficiency across various sugar contents and thus can be applied to a wide variety of breads with differing sugar levels. Furthermore, the strain of the present invention shows reduced fermentation activity at low temperatures, which leads to minimal quality changes during refrigerated distribution, and thus is suitable for refrigerated dough distribution. Additionally, the dough made using the strain of the present invention retains its superior fermentation capabilities even after being stored in a frozen state and then thawed, and thus is suitable for frozen dough distribution.

The invention discloses a preparation method of a cereal fermentation product and a cosmetic. The preparation method of the grain fermentation product comprises the steps of saccharomycetes cultivation, culture medium preparation, fermentation, purification, compounding, filtration and the like. Wherein high-activity strains are screened by adopting high and low temperature circulating treatment and volcanic rock-arid saline-alkali soil environment treatment stress simulation modes in the saccharomycetes culture; the culture medium is compounded with yeast powder, glucose and various cereals such as rice, barley seeds, rice seeds, buckwheat seeds, millet seeds and the like according to a specific proportion. The cereal leavening prepared by the preparation method disclosed by the invention is rich in natural components and high in safety, has excellent moisturizing, anti-oxidation and repairing effects, and can be used for preparing high-performance cosmetics.

The invention belongs to the technical field of resources and environmental microorganisms, and discloses Kluyveromyces marxianus, a method and application of the Kluyveromyces marxianus, the name is ART-27, the classification name is Kluyveromyces marxianus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.39119, and the preservation date is January 7, 2026. The Kluyveromyces marxianus can flocculate macromolecular protein in dairy product water and utilize the lactose, the protein and other components to improve the utilization rate of the lactose and the protein, and the degradation rate of the Kluyveromyces marxianus to the lactose is 70.20% and is improved by 20.20% compared with the degradation rate of 50% of original bacteria; the degradation rate of whey protein is 76.20%, and is increased by 22.2% compared with the degradation rate 54% of original bacteria. The method has a good application prospect in the aspects of dairy product water treatment and resource utilization.