244 results about "Yeast strain" patented technology

The invention belongs to the field of food microorganisms, and particularly relates to a saccharomycopsis fibuligera strain, a microbial inoculum, a fermented product and preparation and application thereof. The saccharomycopsis fibuligera strain provided by the invention is preserved in the China Center for Type Culture Collection on September 25, 2023, and the preservation number of the saccharomycopsis fibuligera strain is CCTCC NO: M 20231788. The saccharomycopsis fibuligera strain provided by the invention can produce amylase, protease, cellulase and other enzymes required by fermentation, the amylase has the activity of pullulanase and raw amylase and can well adapt to the special environmental conditions of fermentation, and the saccharomycopsis fibuligera strain has high environmental tolerance and high aroma-producing capacity; therefore, the quality of fermented products is improved, and the fermentation production investment cost is reduced.

The invention provides noodles with a low glycemic index (GI) and a preparation method thereof, and relates to the technical field of food processing. The low-GI noodles provided by the invention are prepared from the following raw materials in parts by mass: 5-10 parts of kale powder, 8-14 parts of bean dreg and wheat bran powder, 75-85 parts of flour and other auxiliary materials, and the bean dreg and wheat bran powder is prepared by mixing a yeast strain in a laboratory with dry bean dregs and dry wheat bran (in a mass ratio of 1: 1), fermenting and then carrying out superfine grinding. The noodles provided by the invention are low-glycemic-index food, have the advantages of low sugar, low fat, low salt, high fiber, moderate hardness, good elasticity and uniform tissue structure, are simple and convenient in processing method and high in product stability, are suitable for serving as staple food to be applied to diet management of diabetes and obesity, are beneficial to improving the blood sugar level of related people, and are suitable for being eaten by people with diabetes and obesity. The overall health condition of citizens is improved.

Provided herein are methods and materials for using organisms (e.g., engineered yeast strain-derived) to generate lipid compositions which can modulate cellular aging, extend lifespan, and / or modulate gut homeostasis in a subject. The lipid compositions contain at least twelve of the lipids selected from a phosphatidylethanolamine (PE); a lysophosphatidylethanolamine (LPE); a diacylglycerol (DG); a monoglyceride (MG); an acyl carnitine (AcCa); a phosphatidic acid (PA); a phosphatidylglycerol (PG); a lysophosphatidylcholine (LPC); a triacylglycerol (TG); a cholesterol ester (ChE); a wax ester (WE); a phosphatidylserine (PS); a phosphatidylcholine (PC); a phosphatidylinositol (PI); a ceramide (Cer); and a lysophosphatidylserine (LPS).

The invention belongs to the technical field of microbial fermentation, and particularly relates to Wickerhamomyces anomalus and application thereof in preparation of ethyl palmitate, propyl linoleate or ethyl oleate. According to the present invention, the Wickerhamomyces anomalus is provided, the Wickerhamomyces anomalus is the Wickerhamomyces anomalus Lz5, the classification name of the Wickerhamomyces anomalus is Wickerhamomyces anomalus, the Wickerhamomyces anomalus is preserved in the China General Microbiological Culture Collection Center on June 19, 2025, and the preservation number of the Wickerhamomyces anomalus is CGMCC No. 34954; the address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing; when the strain is used for fermenting sugarcane juice, ethyl palmitate, propyl linoleate and ethyl oleate can be prepared. In the culture medium after fermentation is finished, the content of ethyl palmitate can reach 13.68 mg / L. After subsequent extraction, the method can be used for industrial production.

The invention provides yeast HAU2501 and application thereof in preparation of fermented vegetables, and belongs to the technical field of fermentation microorganisms. The invention discloses saccharomycetes HAU2501, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 21, 2025, and the preservation number is CGMCC No.35320. The saccharomycetes HAU2501 has the advantages that the saccharomycetes HAU2501 can be used for preparing the saccharomycetes HAU2501; the saccharomycetes HAU2501 can be used as an excellent aroma-producing leavening agent, can effectively improve the flavor quality of the fermented vegetables through synergistic fermentation with lactic acid bacteria, and provides a theoretical basis and a technical reference for flavor regulation and control of traditional fermented vegetables and development of functional leavening agents.

This invention relates to a highly stress-tolerant cyst-forming yeast and its application in aged vinegar production, belonging to the field of vinegar brewing technology. To screen for ester-producing yeast strains with high stress tolerance and apply them to the aged vinegar brewing process, this invention isolated a yeast strain ZLCY-93 from the fermentation mash and vinegar mash of Shanxi aged vinegar. This strain exhibits high enzyme production, high ester production capacity, stronger adaptability to high temperature, high acidity, high osmotic pressure, and high concentration of ethanol during fermentation, good fermentation performance, and outstanding aroma production. Morphological and molecular biological analysis identified this yeast strain as a cyst-forming yeast. This invention combines yeast ZLCY-93 with practical experience in aged vinegar production to create bran koji, which, when applied to aged vinegar production, can shorten the fermentation cycle, improve raw material utilization, promote flavor compound formation, and enhance product quality.

The invention belongs to the technical field of bioengineering, and relates to industrial microorganism breeding, in particular to a high-yield ethyl acetate diploid / tetraploid saccharomyces cerevisiae strain as well as a construction method and application thereof. According to the research, a diploid / tetraploid saccharomyces cerevisiae strain is constructed on the basis of an existing high-yield ethyl acetate haploid yeast strain by utilizing a CRISPR / Cas9 mediated mating type conversion technology. And a series of diploid / tetraploid saccharomyces cerevisiae strains with the acetic acid and lactic acid tolerance and the ethyl acetate yield obviously improved compared with the starting strain are obtained. According to the series of strains, ethyl acetate is high in yield while the excellent alcoholic fermentation characteristic is kept in the fermentation process, white spirit fermented by the series of strains is more prominent in ester fragrance and grain fragrance, weaker in foreign flavor and better in taste on the basis of guaranteeing the original style, a new thought is provided for the breeding mode of yeast strains, and the method is worthy of popularization and application. And a reference is provided for production optimization of ethyl acetate in a Baijiu fermentation process.

The present invention provides an engineered strain of yeast having mitochondrion-positioned reductive TCA pathway and efficiently producing succinic acid, a construction method therefor and use thereof, and belongs to the technical field of microorganisms and fermentation engineering. According to the present invention, the enzymes related to the biosynthesis pathway of succinic acid in yeast cells are overexpressed and positioned to the mitochondrial matrix to facilitate the functional expression of the reductive TCA pathway in eukaryotic microorganisms. The strain can utilize common carbon and nitrogen sources, and after fed-batch fermentation culture, the succinic acid yield reaches 50.1 g / L, and the conversion rate reaches 0.75 g / g of glucose. As an excellent strain for efficiently producing succinic acid, the strain has good application prospects in the fields of food, chemical engineering, degradable materials, and the like.

A yeast cultivation medium and method of yeast cultivation are provided. The yeast cultivation medium can be an aqueous solution including molasses, rice water, and at least one hydrolytic enzyme, which can be a hydrolase. The yeast cultivation method can include adding a yeast strain to the yeast cultivation medium, heating the yeast-containing cultivation medium, and extracting the yeast from the cultivation medium.

The invention relates to the technical field of biology, in particular to yeast hydrolysate and application thereof. According to the method, enzymes are reasonably screened, the obtained compound enzyme can conduct enzymolysis on yeast strains more effectively, so that hydrolysate rich in free-state lipid, amino acid, polysaccharide and other substances is prepared, the hydrolysate is more beneficial to growth and utilization of yeast, the health state of active yeast cell membranes is improved, and yeast fermentation is promoted.

The invention belongs to the field of microbial fermentation and the technical field of daily chemicals, and particularly relates to deacetylated sophorolipid as well as a preparation method and application thereof. By using cheap culture medium components and screening out saccharomycetes with high yield of lactone type sophorolipid, the problem that the production cost of the sophorolipid is too high is effectively solved, and the application of the sophorolipid in various fields is promoted; through the step of carrying out enzymolysis on a fermentation substrate in advance, the problems of long single fermentation period, low thallus utilization rate, high energy consumption and high cost of traditional saccharomycetes are solved. A multi-round fermentation circulation system is designed, and bacteria liquid is settled and recycled and then inoculated and fermented, so that thalli are efficiently and repeatedly utilized, the unit yield is increased, the fermentation period is shortened, and the production cost is effectively controlled. The structure is adjusted through a resin modification technology, a final product mainly comprises acetylated sophorolipid, and compared with acetylated sophorolipid and deacetylated sophorolipid, the sophorolipid has better foam stability and sensitive skin repairing effect and is suitable for the application fields of cosmetics, washing care and the like.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V based on multi-compartment multi-metabolic pathways and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The preparation method comprises the following steps: anchoring an MVA pathway and a squalene synthesis pathway on the surface of a peroxisome membrane, and enabling mogrol synthetase and epoxy squalene cyclization enzyme ERG1 to form a linear polywheat complex through polypeptide interaction; then, through overexpression of MVA pathway key enzyme and introduction of an isopentenol utilization pathway, supply of precursor isopentenyl pyrophosphate and dimethyl allyl pyrophosphate in cytoplasm is increased, and squalene epoxidase ERG1 and mogrol synthetase are anchored in transmembrane ABC efflux protein PDR11 through polypeptide interaction; then anchoring the mogrol synthetase in the lipid droplet subcells; finally, the glucosyltransferase is displayed on the surface of the yeast cell wall through an alpha-lectin system, so that extracellular mogrol is converted into MG-V. The engineering bacterium improves the yield of mogroside V synthesized from the beginning in microorganisms, and has great industrial application value.

The invention discloses a Kluyveromyces marxianus strain and an application of the Kluyveromyces marxianus strain, and belongs to the field of synthetic biology and microorganism manufacturing. The strain has the characteristics of efficiently utilizing xylose and growing under a low-oxygen condition, and by taking the strain as a fermentation strain, the protein content of fermented straw powder is remarkably improved through the synergistic effect of the strain and cellulase under a straw powder high-solid fermentation condition. According to the yeast strain and the high-solid fermentation process thereof disclosed by the invention, the protein content of straw powder can be greatly increased, and an effective solution is provided for high-valued reutilization of straw agricultural wastes.

The application discloses a strain of yeast for efficiently transforming ginsenosides and polysaccharides and application thereof, and belongs to the technical field of functional component transformation. According to the total ginsenoside and polysaccharide content in ginseng, a strain of yeast for efficiently transforming ginsenosides and polysaccharides is screened by taking a comprehensive score as an index, and is identified as Zygosaccharomyces bisporus by morphology, physiology and biochemistry and molecular biology, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2020912. After transformation, the total ginsenoside content in American ginseng and ginseng is respectively increased by 67.81% and 107.03%, and the polysaccharide content is respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity of the total ginsenoside extract and polysaccharide extract of the American ginseng and ginseng after transformation are all improved. The yeast screened by the application has a wide application prospect.

This invention aims to create a spatially isolated local reaction microenvironment for Rubisco by introducing a complete β-carboxysome microcompartment system. This design goal avoids oxygen inhibition, laying the foundation for ultimately achieving efficient and specific CO2 fixation. It provides a novel approach to overcome the fundamental problem of low efficiency of carbon-fixing enzymes in cytosols due to intense oxygen competition. By introducing the complete carboxysome system as a module, engineered yeast strains with high carboxylase activity and significantly improved ethanol production were obtained, providing a solid foundation for building next-generation yeast cell factories that produce high-yield ethanol using CO2 as a raw material.

The present disclosure relates to the field of bioengineered yeast strains as therapeutic platforms. In particular, engineered strains of yeast brassicae comprising a nucleic acid encoding a modified 3-isopropylmalate dehydrogenase (Leu2) and a therapeutic protein, and methods for treating or preventing a disease or condition in a subject in need thereof.

The embodiment of the utility model provides a saccharomycetes strain constant-temperature preservation device for resisting oxidation, and relates to the technical field of saccharomycetes preservation. The yeast strain constant-temperature preservation device for oxidation resistance comprises a preservation box body, a box door is movably connected to the front side surface of the preservation box body, a plurality of heat dissipation holes are formed in the two side surfaces of the preservation box body, constant-temperature devices are fixedly installed on the left side and the right side of the preservation box body, a preservation mechanism is arranged in the preservation box body, and the heat dissipation holes are formed in the left side and the right side of the preservation box body. And a heating mechanism is fixedly mounted on the surface of the back side of the storage box body. According to the embodiment of the invention, the thermal insulation pipe is used for storing strains, the limiting clamping plate is pushed to move along the outer surface direction of the thermal insulation pipe by rotating the adjusting screw rod, the extension spring is stretched, and meanwhile, the anti-skid pad and the outer wall surface of the thermal insulation pipe are attached and clamped, so that the clamping effect on the thermal insulation pipe is improved by improving the anti-skid effect; therefore, the preservation effect on the strain is improved.

The invention relates to the technical field of microbial screening and fermentation, in particular to saccharomycetes for refrigerated dough and application of the saccharomycetes. The Saccharomyces cerevisiae strain is Saccharomyces cerevisiae AMCC31711, and the preservation number of the Saccharomyces cerevisiae strain is CCTCC (China Center for Type Culture Collection) NO: M 2024384. The yeast strain obtained by the invention has low-temperature sensitivity, the activity of the yeast at low temperature is greatly reduced compared with that of an original strain, the influence on dough in a refrigeration process is reduced, the state of the dough is favorably kept, and the problems of swelling, fragility and the like caused by the traditional yeast in the refrigeration process are avoided; meanwhile, after the temperature is recovered to 30 DEG C, the fermentation activity of the strain is rapidly improved, so that the dough can be rapidly fermented, and the potential in cold storage dough production is huge.

The invention relates to the technical field of gene screening, and discloses a method for screening a cadmium-resistant gene of spanishneedles herb by using a yeast strain ycf1, and the method comprises the following steps: S1, carrying out no-load plasmid transformation on recipient bacteria ycf1; s2, transforming recipient bacteria ycf1 in a spanishneedles herb library; s3, preparing a yeast working bacterial liquid; and S4, cadmium concentration screening. By adopting yeast screening, the interaction can be simulated in a cell environment close to a natural state, and the method has the following advantages that high-throughput screening capability is realized, and a large number of samples can be rapidly screened; convenience and operability are achieved, and the method is suitable for being widely applied to different laboratories; the stability is realized, the interaction research in yeast cells is more stable, and the influence of the environment on the experimental result is reduced.

The present invention relates to the use of an isolated Aureobasidium pullulans yeast strain YBCA5 as a biological control agent. Processes and compositions for the biological control of phytopathogenic bacteria and fungi using YBCA5 are also provided.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The invention belongs to the technical field of microorganisms, and particularly relates to a compound fermentation inoculant for increasing the relative content of ethyl decanoate in roxburgh rose fruit wine and application. According to the present invention, two saccharomycetes are separated from a wild rosa roxburghii fermentation broth, i.e., a Starmerella bacillus and a Saccharomyces cerevisiae, and are compounded to obtain the composite fermentation bacterial agent, and the bacterial agent can produce ethyl decanoate at high yield and is applied to rosa roxburghii fruit wine brewing, such that the relative content of ethyl decanoate in the rosa roxburghii fruit wine is increased, and the yield of the rosa roxburghii fruit wine is increased; the flavor quality of the roxburgh rose fruit wine is improved, and technical support is provided for industrial development of the roxburgh rose fruit wine. When the composite microbial agent is used for fermenting the rosa roxburghii tratt fruit wine, the flavor and taste of the rosa roxburghii tratt fruit wine can be remarkably improved, the relative content of ester substances in volatile flavor substances of the rosa roxburghii tratt fruit wine is increased by 68.527% in total, and the highest relative content of ethyl decanoate is 26.551%. In addition, the relative content of ethyl laurate and the relative content of ethyl caprylate are increased to 14.228% and 11.335% respectively.

The invention relates to the technical field of microorganisms, in particular to a yeast metagen with an anti-allergic effect as well as a preparation method and application of the yeast metagen. According to the invention, the metabolite is an inactivated saccharomyces boulardii strain and a fermentation metabolite thereof, wherein the preservation number of the saccharomyces boulardii strain is CCTCC (China Center for Type Culture Collection) NO: M 20251803. The metagen can be used for remarkably reducing the total IgE (Immunoglobulin E) level of serum and relieving anaphylactic reaction by adjusting the balance of Th1 / Th2, Th17 and Treg immune cells. Therefore, the metabiotics disclosed by the invention have a wide application prospect in preparation of foods, medicines or health-care products for relieving allergic symptoms.

The invention belongs to the technical field of microorganisms, and relates to schizosaccharomyces japonicus capable of efficiently metabolizing malic acid and an application of the schizosaccharomyces japonicus. The schizosaccharomyces japonicus strain FBKL2.9SZJ-37 capable of efficiently metabolizing malic acid is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20251245, the preservation time is June 4, 2025, the strain shows the efficient malic acid metabolizing ability during fermentation, 2.959 g / L of malic acid is consumed after the strain is cultured in an MDL screening culture medium for 7 days, and the highest degradation rate in all strains is 98.64%; 2.965 g / L of malic acid is consumed after fermentation of a simulated malic acid fermentation medium (SMF) for 16 days, and the degradation rate of malic acid is 98.83%; the malic acid consumption after fermentation of the cabernet sauvignon, the rosa sterilis and the rosa roxburghii tratt is 2.93 g / L, 28.73 g / L and 32.40 g / L respectively, and the malic acid degradation rates are 98.32%, 60.50% and 64.98% respectively. The strain is excellent in fermentation performance, stable in ethanol yield, high in tolerance, relatively high in glycerol yield and relatively low in volatile acid level.

The invention discloses trichosporon arystachys capable of reducing ammonia nitrogen and nitrite and application of the trichosporon arystachys. The saccharomycetes are named as Trichosporonashii JM-1, and the preservation number of the saccharomycetes is GDMCC No: 64448. The invention further discloses a preparation method of the saccharomycetes. The Saccharomyces cerevisiae grows well in a high-concentration ammonia nitrogen and nitrite environment, is high in nitrogen degradation efficiency and good in environmental adaptability, can be used as a functional strain for improving water quality, deodorizing farms, deodorizing garbage yards and the like, is applied to multiple places, reduces nitrogen accumulation, protects the environment, has certain degradation capability on cellulose, and can be used as a functional strain for improving the water quality, deodorizing the farms, deodorizing the garbage yards and the like. The method has great application potential in livestock and poultry aquaculture and environmental protection.

Disclosed herein are yeast strains and derivatives thereof, as well as compositions comprising the yeast strains for use in ethanol production. The disclosure also relates to processes for producing ethanol from biomass using the yeast strains and compositions. In particular, the yeast strains produce less glycerol and more ethanol, and have higher temperature tolerance and faster fermentation rates than strains and products currently used in ethanol production processes.

A yeast biomass composition is formed by culturing a strain of yeast in a substrate such as whey permeate to product a yeast broth, dewatering the broth to produce yeast biomass, and hydrolysing the yeast biomass. The yeast biomass composition finds utility as a feed additive for broiler chickens to reduce the adherence of pathogenic bacteria like Salmonella and E.coli to the gut wall of the chickens, thereby reducing intestinal stress and improving life daily weight gain in the chickens.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

Disclosed are bacterial strain HSCY 2073, isolation and screening thereof, and application thereof in improvement on flavor quality of vinegar. Bacterial strain HSCY 2073 is identified as Lactobacillus sp. and is deposited in China General Microbiological Culture Collection Center, where deposit date is 21 November 2019, and deposit number is CGMCC No. 18982. Bacterial strain HSCY 2073 in the present disclosure is Lactobacillus sp. that has strong acetic acid and ethanol tolerance and excellent effect of producing non-volatile acid, and can produce high contents of 5 aroma substances including acetaldehyde, ethyl acetate, isobutyl acetate, isoamyl acetate and ethyl 3-methylvalerate that have fruit aromas. The lactobacillus can adapt to high-acid, high-alcohol and oligotrophic harsh environments, etc. of vinegar brewing, and has strong ability of high acid tolerance.