31 results about "Selenocysteine" patented technology

This invention discloses a selenylamide-based hydrogenation reaction of acetylacetamide compounds and its application in the modification of selenium-containing peptides and proteins, belonging to the fields of organic chemistry and chemical biology. The reaction involves the reaction of a selenium-containing compound with an acetylacetamide compound in a solvent with the addition of an additive. The applications of this reaction include: 1) selective modification and labeling of selenocysteine ​​in peptides and proteins; 2) cyclization reactions of selenium-containing peptides and selenoproteins; and 3) sequential modification of selenium-containing peptides in the presence of cysteine ​​by precise pH control combined with the hydrogen sulfide reaction of acetylacetamide. This reaction has advantages such as mild conditions, simple operation, fast reaction rate, high yield, good stability, and good selectivity. It provides a new and robust approach for the selective modification and labeling of selenium groups in bioconjugations, peptides, and / or proteins, and also provides a new method for the construction of cyclic peptides, offering a powerful tool for the development of selenium-containing peptide drugs.

The present application relates to the chemical medicine field, specifically, it relates to a kind of anti-tumor calophyllol derivative, preparation method and application, the calophyllol derivative has good inhibitory effect on thioredoxin reductase activity, and it is found that the molecule inhibits the activity of thioredoxin reductase by inhibiting the selenium cysteine of thioredoxin reductase carbon end, and then kills tumor cell, and then obtain the anti-tumor candidate drug with higher activity and better pharmacokinetic characteristics.

The application discloses a Limosilactobacillus reuteri strain capable of improving selenoenzyme activity and high in selenium selenocysteine, and belongs to the technical field of microorganisms.The Limosilactobacillus reuteri CCFM1303 screened by the application can efficiently enrich inorganic selenium and convert the inorganic selenium into organic selenium (selenium selenocysteine), which can be better absorbed and utilized by a body, and effectively improve selenoenzyme activity of the body.Animal experiments prove that the selenium-rich Limosilactobacillus reuteri can improve selenoenzyme activity in male mice, has higher biological activity than inorganic selenium, and meets physiological requirements of selenium supplement.The Limosilactobacillus reuteri CCFM1303 of the application can efficiently enrich inorganic selenium, produce selenium selenocysteine, and be used for preparing probiotic preparations for improving selenoenzyme activity of a body, and has great application prospects in the fields of food and medicine.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

This invention belongs to the field of microbial technology and provides a space-mutated *Bacillus paralichrysiformis* strain with high synthetic selenocysteine ​​and high sodium selenite tolerance, obtained through space mutagenesis, and its applications. The *Bacillus paralichrysiformis* G1-m strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36525, deposited on November 11, 2025. This strain has significant core advantages: firstly, it possesses extremely high sodium selenite tolerance, able to withstand sodium selenite stress up to 440 mM; secondly, its selenocysteine ​​and nano-selenium synthesis efficiency is significantly improved, not only significantly increasing the strain's resistance threshold to selenium stress but also directionally enhancing the activity of the selenocysteine ​​biosynthetic pathway. Its application potential covers multiple fields, such as selenium-enriched probiotic preparations, nano-drug carriers, functional food additives, and high-efficiency selenium nutritional supplements, laying the foundation for the high-value utilization of selenium resources and the industrial application of functional microorganisms.

The invention relates to the technical field of traditional Chinese medicine extract fermentation, and provides a microspore ganoderma lucidum immune protein and herba houttuyniae fermentation method for treating AIDS and application of microspore ganoderma lucidum immune protein and herba houttuyniae fermentation method.The microspore ganoderma lucidum immune protein and herba houttuyniae fermentation method comprises the steps that microspore ganoderma lucidum strains and herba houttuyniae fermentation liquor are co-fermented, beta-cyclodextrin-selenocystine of a specially-made additive is combined, and the microspore ganoderma lucidum immune protein is obtained; the problems that in traditional AIDS treatment, latent infection cells are difficult to remove, toxic and side effects of drugs are remarkable, and the reconstruction efficiency of an immune system is low are effectively solved, experiments prove that the fermentation product can activate the immune system, the cell number and the dendritic cell maturity are remarkably improved, and the curative effect is good. Meanwhile, the multi-target antiviral effect is achieved by blocking virus envelope combination, inhibiting reverse transcriptase activity and inducing latent infection cell apoptosis; the anti-oxidation and anti-inflammatory characteristics can reduce the risk of opportunistic infection, reduce the toxic damage of antiviral drugs to liver, kidney and intestinal tracts, and provide a new scheme for comprehensive treatment of AIDS and nutrition support of patients.

The present invention provides a compound having the structure:A-B-----Zwherein A is a biologically active structure of the compound;wherein Z is a protein component of the compound, which protein component comprises one or more polypeptides, wherein at least one of the one or more polypeptides comprises consecutive amino acids which (i) are identical to a stretch of consecutive amino acids present in a chain of an Fc domain of an antibody; (ii) bind to an Fc receptor; and (iii) have at their N-terminus a sequence selected from the group consisting of a cysteine or selenocysteine; wherein the dashed line between B and Z represents a peptidyl linkage; and wherein the solid line between A and B represents a nonpeptidyl linkage, as well as intermediates dimers thereof, and processes of producing the compounds of the invention.

The invention discloses a degradable magnesium alloy intravascular stent based on bionic hydrogel and nitric oxide double-path release and a preparation method and application thereof. The method comprises the steps that a magnesium alloy base material is subjected to alkali heat treatment; immersing the treated magnesium alloy into a silane coupling agent for reaction; the photopolymerization reaction liquid is treated to the surface of the silanization modified magnesium alloy, and polymerization is conducted through ultraviolet irradiation; immersing the magnesium alloy with the hydrogel coating into a polyarginine solution, and grafting polyarginine onto the surface of the hydrogel; a sample is activated through EDC / NHS and then immersed in a selenocystamine solution for a reaction, and the bionic composite coating, with the exogenous / endogenous dual-path nitric oxide release function, of the degradable magnesium alloy intravascular stent is obtained. The invention successfully constructs the bionic coating integrating cell membrane-imitating anti-fouling and exogenous / endogenous dual-path NO release functions, and the coating effectively solves the key problems of corrosion control, thrombus formation resistance, functional re-endothelialization coordinated regulation and the like faced by the degradable magnesium alloy intravascular stent.

The application discloses a kind of pectinatocarpus selenium protein peptide and albumin peptide formula and preparation method, the core active ingredient of the formula is the selenium protein peptide of pectinatocarpus source and egg white albumin peptide, wherein the number average molecular weight of selenium protein peptide is 461Da, total selenium is 412.67mg / kg and selenocysteine and pectinatocarpus plant selenium peptide ratio is more than 90%, and the biological potency of albumin peptide is 94, and the molecular weight is 200-800Da;The application promotes active transport in intestinal tract by albumin peptide as carrier, and is prepared by combining dry granulation and tabletting process, the selenium absorption rate of the application is greatly improved, realizes the synergistic effect of hypoglycemic, hypolipidemic and antioxidant, and provides a new scheme for selenium supplement functional food.

The invention discloses a ratio type near-infrared fluorescent probe Dn-NIR-Sec of selenocysteine as well as a preparation method and application of the ratio type near-infrared fluorescent probe Dn-NIR-Sec. The chemical structural formula of the ratio type near-infrared fluorescent probe Dn-NIR-Sec is shown as a formula (I). The preparation method comprises the following steps: condensing a compound 1 shown as a structural formula (1) with cyclohexanone under an acidic condition to obtain a compound 2 shown as a structural formula (2); the preparation method comprises the following steps: firstly, preparing a compound 2, then condensing the compound 2 and a compound 3 with a structural formula as shown in a formula (3) to obtain a ratio-type near-infrared dye platform Dn-NIR-OH, and finally reacting with 2, 4-dinitrobenzenesulfonyl chloride to obtain the ratio-type near-infrared dye platform Dn-NIR-OH. Experimental results show that the ratio-type near-infrared fluorescent probe Dn-NIR-Sec can complete rapid fluorescent response to Sec within 5 minutes, real-time dynamic detection is facilitated, selective recognition performance on selenocysteine is remarkable, light stability is good, and meanwhile the ratio-type near-infrared fluorescent probe Dn-NIR-Sec has good cell membrane permeability and low cytotoxicity. Therefore, the probe can be widely applied to the fields of biological detection and the like as a fluorescence detection tool.

The present invention relates to a method for the enzymatic synthesis of (oligo)peptides comprising coupling (a) a (oligo)peptide C-terminal ester or thioester and (b) a (oligo)peptide nucleophile having an N-terminal unprotected amine, wherein the coupling is performed in a fluid comprising water, and wherein the coupling is catalyzed by a subtilisin BPN' variant or a homologue thereof comprising the following mutations compared to subtilisin BPN' as set forth in SEQUENCE ID NO: 2: deletion of the amino acids corresponding to positions 75-83; a mutation at the amino acid position corresponding to S221 which is S221C or S221 selenocysteine; preferably a mutation at the amino acid position corresponding to P225, wherein the amino acid positions are defined according to the sequence of subtilisin BPN' as set forth in SEQUENCE ID NO: 2. In addition, the present invention relates to enzymes suitable for use as catalysts in the method of the present invention.

A selenium-enriched biotin scalp care essence spray and its preparation method belong to the field of scalp care technology. The spray components are: selenium yeast extract, pearl active peptides, hair follicle repair core enzyme complex, biotin, plant extract micropowder, and the remainder being excipients and deionized water, with a pH of 5.0-5.5. The selenium yeast extract contains ≥60% selenocysteine; the pearl active peptides have a molecular weight <1kDa and an amino acid content ≥90mg / mL; the hair follicle repair core enzyme complex contains ≥2000U / mg superoxide dismutase and ≥50U / mg transglutaminase; and the plant extract micropowder is extracted using supercritical CO2. The use of ultrasonic high-pressure homogenization, iontophoresis encapsulation, and nitrogen-filled aluminum bottle packaging technology solves the problems of low transdermal penetration rate of active ingredients (traditional formulations <8%, this invention increases it to 28%) and poor stability.

A general-purpose selenium-enriched pearl-based basic skincare mother liquor, by weight, is composed of pearl active peptides, selenium yeast extract, and an enzyme complex in a weight ratio of 1:(0.00625~0.1):(0.0375~0.25). The pearl active peptides have a molecular weight <1kDa, an amino acid content ≥90mg / mL, and are colorless and transparent. The selenium yeast extract has a particle size <80nm, selenocysteine ​​≥60%, and a Zeta potential of +18 to +23mV, and is a colorless and transparent dispersion. The enzyme complex contains SOD ≥2000U / mg and CAT ≥1500U / mg, and is encapsulated in a chitosan-sodium alginate bilayer, making it colorless and semi-transparent. This basic skincare mother liquor addresses the problems of low dissolution and delivery rates of pearl's effective components, poor stability of selenium components, and lack of multi-effect synergistic mechanisms. It can be used to rapidly prepare various types of cosmetics, such as serums (for external or oral use) and masks.

The present application relates to the technical field of agricultural product quality and safety detection and element form analysis, and particularly relates to an LC-ICP-MS / MS method for detecting selenocysteine. The present application provides an LC-ICP-MS / MS method for detecting selenocysteine, wherein the sample to be detected is mixed with iodine acetamide solution and buffer extraction solution for enzymolysis to obtain an enzymolysis solution; the final concentration of iodine acetamide in the mixed solution is 0.02-0.10 mmol / L; the purified solution is injected into a liquid chromatography system for high performance liquid chromatography separation, a solution without ion pair reagent, with pH buffering capacity and excellent ICP-MS compatibility is used as a mobile phase, and an anion exchange chromatographic column is used as a chromatographic column; the chromatographic effluent is introduced into ICP-MS / MS for detection, CH4 is used as a reaction gas, a detection method with a recovery rate of greater than or equal to 90% and no residual interference is constructed, and a new paradigm is provided for the accurate analysis of unstable selenium forms.

The invention provides a rhodamine derivative fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of fluorescence detection. The preparation method comprises the following steps: mixing rhodamine 110 conjugated PEG azide, triethylamine and anhydrous dichloromethane to obtain a mixed solution; and dropwise adding a 2, 4-dinitrobenzenesulfonyl chloride solution into the mixed solution, reacting at low temperature, and reacting at normal temperature in a dark place to obtain the rhodamine derivative fluorescent probe. The rhodamine derivative fluorescent probe shows good stability when being used for detecting the selenocysteine, has low detection limit on the selenocysteine, has high sensitivity and good selectivity, and is suitable for detecting the selenocysteine in livestock and poultry products.

The present invention provides a compound having the structure: wherein A is a biologically active structure of the compound; wherein Z is a protein component of the compound, which protein component comprises one or more polypeptides, wherein at least one of the one or more polypeptides comprises consecutive amino acids which (i) are identical to a stretch of consecutive amino acids present in a chain of an Fc domain of an antibody; (ii) bind to an Fc receptor; and (iii) have at their N-terminus a sequence selected from the group consisting of a cysteine or selenocysteine; wherein the dashed line between B and Z represents a peptidyl linkage; and wherein the solid line between A and B represents a nonpeptidyl linkage, as well as intermediates dimers thereof, and processes of producing the compounds of the invention.

A conditioner for improving a soil environment belongs to the field of soil environment improvement and comprises the following substances in parts by weight: 6-8 parts of activated calcium silicate, 12-15 parts of red mud, 3-5 parts of selenocysteine, 4-6 parts of a composite decomposition microbial agent, 3-5 parts of boric acid, 4-6 parts of ferrous sulfate, 6-8 parts of aminated alginic acid, 15-20 parts of monopotassium phosphate, 6-10 parts of plant ash and 3-5 parts of chitosan. Through the synergistic effect of all the raw materials, heavy metals in soil can be efficiently fixed and removed from multiple dimensions, meanwhile, rich nutrient elements and beneficial microorganisms are supplemented for the soil, on the basis of reducing the bioavailability of the heavy metals and relieving the harm of the heavy metals to the ecological environment, the physicochemical properties and the micro-ecological structure of the soil are effectively improved, and the soil quality is improved. The soil remediation method has the advantages of being easy and convenient to operate, lasting in remediation effect, free of secondary pollution and the like, and is suitable for improvement of soil polluted by multiple types of heavy metals.

The invention discloses an injectable porous selenium-loaded antibacterial bone cement material and a preparation method thereof, and relates to the field of biomedical materials. The bone cement material is prepared from solid-phase powder and curing liquid, the solid-phase powder comprises beta-calcium phosphate, monocalcium phosphate monohydrate, selenocystine, bioactive glass, calcium carbonate, magnesium powder, stearic acid and carbon powder; the curing liquid is a sodium hyaluronate solution; the preparation method comprises the following steps: grinding magnesium powder and stearic acid, adding carbon powder, and continuously grinding to obtain composite pore-forming agent powder; the preparation method comprises the following steps: grinding and mixing beta-calcium phosphate, monocalcium phosphate monohydrate and selenocystine to obtain antibacterial matrix powder; grinding and mixing the composite pore-forming agent powder, bioactive glass and calcium carbonate to obtain auxiliary functional powder; and mixing and grinding the two powders, adding a sodium hyaluronate curing liquid, uniformly mixing, and curing to obtain the product. The material obtained by the invention has excellent broad-spectrum antibacterial ability, can construct an alkalescent microenvironment to synergistically promote bone repair, and has a good clinical application prospect.

The application discloses a nano drug based on nitrogen-doped selenized carbon dots and a preparation method and application thereof, and belongs to the technical field of biological medicines, wherein the preparation method of the nano drug comprises the following steps: placing an amino acid compound and a small molecule drug in a solvent to mix, adjusting the pH value to be alkaline, then performing a heating reaction to obtain a reaction solution; the amino acid compound is one or more of L-selenocystine, L-selenocysteine and L-selenomethionine; the small molecule drug is metformin hydrochloride or phenformin; and the nano drug is obtained by separating and purifying from the reaction solution. The nano drug can significantly relieve cell oxidative damage induced by hydrogen peroxide, can effectively remove ROS excessively accumulated in a damage site, and can inhibit the progress of secondary damage. In addition, the nano drug can regulate four key signal paths of antioxidation, anti-inflammation, anti-apoptosis and axon regeneration and myelination, and can synergistically promote the nerve repair process after spinal cord injury.

The application discloses a gel factor and a preparation method and application thereof. A structural formula of the gel factor is shown in the following formula: wherein R1 is a side chain group except for an amino group, a carboxyl group and one hydrogen atom connected to a central carbon atom of an amino acid, and the amino acid is selected from one of glycine, alanine, valine, leucine, isoleucine, methionine, proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine and selenocysteine; R2 is an alkyl group with 16-19 carbon atoms; and R3 is a 9-fluorenylmethyloxy carbonyl group or a tert-butyloxy carbonyl group. The gel factor has a hydrophobic long alkyl tail chain, can spontaneously aggregate and assemble into a three-dimensional network structure through hydrogen bonds, pi-pi interaction and hydrophobic force, and can effectively fix oil solvents or oil phases in oil-water mixtures to form a high-strength gel.

The invention relates to the technical field of biological medicines, in particular to an L-selenocysteine selenium nanoparticle as well as preparation and application thereof. The L-selenocysteine selenium nano particle has excellent biocompatibility and an anti-tumor effect, and especially aims at ovarian cancer. Experimental exploration finds that the L-selenocysteine selenium nanoparticles L-Sec SeNPs have an obvious inhibition effect on proliferation of cancer cells, and apoptosis of the cancer cells can be induced by activating a mitochondrial apoptosis line. The L-selenocysteine selenium nano particle disclosed by the invention provides a new thought for treating cancers.

The invention belongs to the technical field of biology, and particularly relates to selenium-containing yolk immunoglobulin with neuroprotection activity and a preparation method and application thereof, and the selenium-containing yolk immunoglobulin contains organic selenium selenocysteine and / or organic selenium selenomethionine. The invention provides selenium-containing egg yolk immunoglobulin and a preparation method thereof, the selenium-containing egg yolk immunoglobulin is screened from selenium-rich egg yolk, the selenium-containing egg yolk immunoglobulin contains selenium, contains organic selenium selenocysteine and selenomethionine, does not contain inorganic selenium and has neuroprotective activity, a secondary structure of the selenium-containing egg yolk immunoglobulin mainly adopts beta folding, and the secondary structure of the selenium-containing egg yolk immunoglobulin mainly adopts beta folding. The protein extract plays a protection role by reducing the NO content and ROS content of BV-2 cells and increasing the GPx content, the SOD content and the CAT content. The selenium-containing yolk immunoglobulin provided by the invention is from food sources, is safe and low-sensitive, and has good neuroprotective activity, a high dose of peptide is suitable for patients with moderate and severe diabetes, and a low dose of peptide is suitable for daily diet supplement of the patients with diabetes in an early stage or a stable stage.

This invention discloses a TaSMT-5B gene for enhancing wheat resistance to Fusarium head blight and its applications. The TaSMT-5B gene belongs to the selenocysteine ​​methyltransferase family, catalyzing the methylation of SeCys to MeSeCys. It is stably and highly expressed in the high-MeSeCys-rich and Fusarium head blight-resistant variety Zheng 9023, and a "GTCGCCG" insertion site exists in the promoter region of this gene in the Zheng 9023 variety. The TaSMT-5B gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in five stable transgenic lines. After basal application of inorganic selenium, the severity of Fusarium head blight in the transgenic plants was alleviated. This invention has significant production implications for achieving selenium biofortification in wheat and enhancing its resistance to Fusarium head blight, providing a new gene resource for breeding stable-yielding and high-quality wheat varieties.

The application provides a synthesis method and application of a selenocysteine-mediated cyclic peptide. A substrate polypeptide is synthesized by a solid-phase polypeptide synthesis method, the C terminal of the substrate polypeptide exists in a hydrazine form, and the N terminal of the substrate polypeptide is selenocysteine. A cyclization method is used to add ascorbate, high-concentration tris(2-chloroethyl) phosphate (TCEP) and acetylacetone to the substrate polypeptide, and the reaction is carried out at room temperature by oscillation to obtain a corresponding target cyclic peptide. The application helps to expand the synthesis method of the cyclic peptide with a ring structure at the head and tail, and realizes efficient synthesis of the cyclic peptide.

The invention provides a Senotrophomonas maltophilia BOF 17 strain and an application of the Senotrophomonas maltophilia BOF 17 strain, and relates to the technical field of microbial growth promotion. The preservation number of the Senotrophomonas maltophilia BOF 17 strain is GDMCC No: 66776, and the preservation number of the Senotrophomonas maltophilia BOF 17 strain is GDMCC No: 66776. The strain not only has a better effect of improving the selenium enrichment of plants, but also can effectively decompose sodium selenite and selenocysteine as selenium sources to promote the growth of the plants, meanwhile further improves the accumulation of minerals such as iron and manganese in plant fruits, effectively improves the quality of the fruits, and comprehensively promotes the economic benefits of plant cultivation.

The application discloses polyglucuronate-selenocystamine phenylboronic pinacol ester or a salt thereof, a preparation method and application. The preparation method comprises the following steps: (1) polyglucuronate or a salt thereof is reacted with selenocystamine to synthesize polyglucuronate-selenocystamine or a salt thereof; (2) polyglucuronate-selenocystamine or the salt thereof is reacted with 4-(hydroxymethyl) phenylboronic pinacol ester through an amide reaction to synthesize polyglucuronate-selenocystamine phenylboronic pinacol ester or a salt thereof; and (3) a protein thrombolytic drug is loaded by an ultrasonic homogenization method to prepare active oxygen-responsive nanogel. The active oxygen-responsive nanogel is combined with P-selectin, so that the active oxygen-responsive nanogel has targeting property for activated platelets at a thrombus site, the purpose of targeted drug delivery and reduced side effects is achieved, the drug is effectively enriched at the thrombus site, the active oxygen microenvironment at an ischemic cerebral stroke site is adjusted, the occurrence of ischemic cerebral stroke infarction is reduced, and a new strategy is provided for clinical treatment of ischemic cerebral stroke.

This invention discloses a method for preparing an infrared-transmitting material. By preparing a doped metal selenium sulfide and organically combining it with graphene, a material is obtained that is opaque in the visible light band (360-750nm) and translucent in the infrared band (750-1100nm), with a transmittance reaching 90%. The main steps include: (1) uniformly dispersing a stabilizer, zinc salt, copper salt, selenium oxide, and L-cysteine ​​in water sequentially; (2) adding a reducing agent solution dropwise under stirring to prepare a pre-product using a hydrothermal method; (3) sintering the pre-product at 900℃ to obtain a doped zinc-copper-selenium sulfide; (4) ultrasonically dispersing the pre-product with graphene oxide in ethanol, and adding vinyltriethoxysilane under acidic conditions to form an infrared-transmitting material. This composite material is simple to prepare, environmentally friendly, has high infrared transmittance, is stable and durable, and has excellent application prospects in information communication, infrared detection, and other fields.

The invention discloses a mitochondrial targeted anti-cancer drug based on cyanine and lonidamine and a preparation method and application thereof, and belongs to the technical field of drugs for treating tumors. Cyanine molecules and 3-mercaptobenzoic acid are subjected to a nucleophilic substitution reaction to prepare a cyanine derivative; the preparation method comprises the following steps: carrying out a coupling reaction on a cyanine derivative and a selenocystamine compound to prepare a cyanine-connexon conjugate with an amino terminal; the cyanine-connexon conjugate and lonidamine are subjected to a coupling reaction, and the targeted anti-cancer drug is obtained. According to the mitochondrial targeting anti-cancer drug based on cyanine and lonidamine and the preparation method and application thereof, after the drug targets tumor cell mitochondria, cyanine molecules generate a photodynamic effect and a photothermal effect at the same time under the irradiation of near-infrared light, ROS and high temperature are generated in situ, the mitochondrial structure and function are directly damaged, and the drug can be used for preparing the mitochondrial targeting anti-cancer drug based on cyanine and lonidamine. The ROS also synchronously triggers diselenide bond breakage, lonidamine is accurately released, and mitochondrial dysfunction and metabolic crisis are further aggravated.

The invention discloses a polypeptide self-assembled nickel-selenium artificial hydrogenase as well as a preparation method and application thereof. The artificial hydrogenase is a polypeptide-nickel compound with a binuclear coordination center, wherein the polypeptide-nickel compound is formed by a self-assembly reaction of polypeptide and nickel ions in a buffer solution; a main chain of the polypeptide provides two strongly coordinated main chain amide groups, polypeptide cysteine and selenocysteine residues provide two side chain mercaptan and / or selenol, and the two side chain mercaptan and / or selenol are coordinated with Ni < 2 + > together to form a binuclear coordination center. The stability of the artificial hydrogenase metal coordination structure is improved, a binuclear metal center can be stably constructed, disordered polypeptide aggregation is reduced, the structural repeatability is improved, the electron transfer path of the metal center is more controllable, and an active structure can be kept under conventional solution and air conditions. An artificial biomimetic catalytic system with a clear structure, good repeatability and higher applicability can be obtained.

The application belongs to the field of biological medicine and nanomaterials, and discloses selenocystine liposome nanomaterial based on cell membrane fusion and its anti-tumor application under cysteine deficiency conditions. The nanomaterial comprises selenocystine liposomes and tumor cell membrane proteins wrapped on the surface of the selenocystine liposomes. The selenocystine liposomes comprise selenocystine, phospholipids, cholesterol and DSPE-MPEG. The application fuses tumor cell membrane proteins with selenocystine-loaded liposomes to construct nanomaterials with tumor targeting and metabolic regulation functions, which can selectively kill tumor cells under cysteine deficiency conditions. The application proves that the selenocystine liposome nanomaterial wrapped with HeLa cell membranes has excellent anti-tumor effect and significantly reduced systemic toxicity in in vitro and in vivo experiments. The application has great clinical application potential.