90 results about "Chemical biology" patented technology

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of human exons. The invention provides the sequences in such a way as to make them available for a variety of analyses including analysis of alternative splicing events. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of at least 5,000 alternatively spliced human genes. As such, the invention relates to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, pharmacology and medical diagnostics.

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of E. coli genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of at least 20,000 E. coli genes and at least 500 intragenic regions. As such, the invention relates to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, and medical diagnostics.

A transfer unit for transferring members such as reaction vessels having an upper end opening or dispensing tips or the like includes at least one arm pivotally movable about a shaft and a holder mounted on the arm for detachably supporting the member to be transferred. The holder is provided with a rod portion having a distal end adapted to be fitted in the upper end opening of the member to be transferred, a rod holding portion slidably and elastically supporting the rod portion and a guide portion surrounding the rod portion thereabout and sliding along the axis of the rod portion to release the fitting of the member from the distal end of the rod portion. The guide portion is formed with at least two elongated apertures extending in parallel with the axis of the rod portion, and the rod portion is provided with protrusions fixed thereto and extending through the elongated apertures to regulate the movement of the guide portion. By applying such a transfer unit to a chemicobiological or immunity analyzing apparatus, the analyzing operation can be performed at a higher speed with a higher efficiency and a particularly compact analyzing apparatus is possible.

Described herein is technology for determining the 2-D or 3-D atomic resolution structure of a polynucleotide bound to and / or interacting with another molecule, for example a small molecule. In some aspects of the technology, NMR and isotopic labeling strategies are used. The technology described herein is useful for a plurality of applications including but not limited to drug discovery and chemical biology probe discovery.

This invention has provided one kind of agricultural chemical biology decompound and its preparation method, it may be medicinal powder, pellet, tablet, liquid, young paste, it is mainly prepared by biological enzyme, stabilizer, adhesive, tax medicinal preparation, chelating agent, active agent through certain craft preparation, it may effective dissolve and decompose fruit vegetables surface and agricultural chemicals in the watery solution in a shorter time, it is one kind of home used and industrial used biological health preparation, which can be applied conveniently and security, and it is green environmental protection.

The invention relates to a process for preparing allocate nanometer modification mini-electric chemical biology sensor used as pesticide residue measurement, which can provide a stable reference electric potential to measure pesticide residue in the vegetables, and an orgyanophosphorus pesticide detecting instrument for pesticide residue measurement.

The invention discloses a method of comprehensive exploitation and utilization on atmospheric carbon resources and CO2 equivalent substance, which is a comprehensive science and technology method by applying physics, chemistry, physical chemistry, biology, biochemistry and the like. Substance such as CO, CO2, hydrocarbons, oxygenated hydrocarbons, hydrofluorocarbon, perfluorocarbon, sulfur hexafluoride and NOx in the atmosphere is captured, sealed, comprehensively developed, deeply processed and recycled, thereby benefiting the mankind, regulating the greenhouse effects, eliminating the haze, mastering the initiative of climate changes, maintaining water-air-ice coexistence climate balance and biodiversity on the earth, creating huge environmental benefits, ecological benefits, social benefits and economic benefits, realizing win-win between economic and social development, environmental protection and climate change handling, and providing inexhaustible new resources and precious wealth for human survival and development.

The invention is a system and method used for teaching and learning computer programming. This system incorporates visualization, plan abstraction and its integration, and language constructs into one teaching and learning environment known as VPCL. There are three phases of VPCL, Plan Observation or Rehearsal, Plan Integration or Composition, and Plan Creation or Innovation. The observation Phase teaches how a program (plan) is broken down into smaller components known as a plan with their relationship. Each plan may further break down into smaller plans. At the last level plans are illustrated by language constructs. The integration phase concentrates on how plans are related to each other to build a program using the four methods of integration. The creation phase concentrates on how a new plan is created using the existing plans along with system resources. VPCL can be applied to other problem solving tasks such as mathematics, science, physics, chemistry, biology, or linguistics.

The present invention is based on the seminal concept of combining genomics and chemical biology to identify new agents useful for induced pluripotent stem cell (iPSC) generation. The invention provides a method of generating an iPSC utilizing agents that antagonize a cell specific gene or upregulate expression or activity of a nuclear reprogramming gene, as well as a method of screening for such agents.

The invention provides a EphB4 acceptor targeting polypeptide and applications thereof, and belongs to the technical fields of radioactive drug labeling and nuclear medicine. A polypeptide (TNYLFSPNGPIARAW, TNYL-RAW for short), which is affinity to EphB4, is taken as the matrix; then the polypeptide is modified by a chemical method and a biological method to obtain a EphB4 targeting molecular probe precursor NOTA-C-(TNYL-RAW); and then the molecular probe precursor is labeled by nuclides for diagnosis or nuclides for treatment to obtain nuclide labeled molecular probe NOTA-c-(TNYL-RAW). The polypeptide can be used to prepare a kit that can be applied to early diagnosis and precise positioning of tumors with high expression of EphB4 and drugs for biological target treatment of tumors with high expression of EphB4.

The invention relates to a preparation method of a disinfecting material namely methyl guanidine chitosan hydrochloride, and belongs to the fields of renewable sources and chemical biology. According to the preparation method, the renewable resources namely chitosan and polyhexamethylene guanidine are used to prepare the disinfecting material. The preparation method comprises the following steps: preparing polyhexamethylene guanidine hydrochloride at first, reacting polyhexamethylene guanidine hydrochloride with glycidyl methacrylate to obtain a polyhexamethylene guanidine hydrochloride-glycidyl methacrylate polymer, and finally reacting the polyhexamethylene guanidine hydrochloride-glycidyl methacrylate polymer with chitosan to obtain methyl guanidine chitosan hydrochloride. The prepared methyl guanidine chitosan hydrochloride can be used as a disinfecting material, which can be used in hospitals, schools, hotels, vehicles, farms, and the like to disinfect the object surfaces and air.

The invention discloses application of kasugamycin and derivatives thereof as a chitinase inhibitor, and a structural formula of the inhibitor is shown in I. Inhibitory effect, selectivity and insecticidal activity of selected compounds are evaluated in an inhibitory activity research, and the results show that the compound Kasugamycin has an inhibitory effect on OfChtI. When final concentration of usage is not less than 50 [mu]M, the inhibitory rate is 86.4% and the half maximal inhibitory concentration (IC50) value is 14 [mu]M. The kasugamycin and derivatives thereof described in the invention have wide application prospects in the fields of biology and chemical biology, especially in delaying the development of Asian corn borers.

The invention discloses a chitinase inhibitor and application thereof. The chitinase inhibitor is first-bit and third-bit derivatives of 2-amino-pyridino-piperidine. The structural formula of the chitinase inhibitor is formula I as shown in the specification. On the basis of inhibition effects and selectivity, the inhibition effect is evaluated and studied for the inhibition activity of compounds,and in all 75 compounds after secondary screening, the first-bit and third-bit derivatives of 2-amino-pyridino-piperidine have certain inhibition activity upon chitinase of human beings, onchocerca volvulus, caenorhabditis elegans, asiatic corn borer, aspergillus fumigates and serratia marcescens. In a word, the first-bit and third-bit derivatives of 2-amino-pyridino-piperidine, which are disclosed by the invention, have wide application prospects in fields such as biology and chemicobiology.

The invention discloses application of berberine and a derivative of the berberine as a hexosaminidase inhibitor. The general structure formula of the hexosaminidase inhibitor is shown as I or II; in all screened 35 compounds, the berberine and the derivative of the berberine show certain inhibition activity on hexosaminidase OfHex1; particularly, the suppression rate of Sysu-00021 and Sysu-00679 on the OfHex1 enzyme is respectively 76 percent and 79 percent, and the inhibition constant Ki is respectively 2mu M and 1.8mu M. Compound applicable object determination shows that under the final concentration of 10mu M, the compound shows higher inhibition activity on the hexosaminidase OfHex1; no inhibition activity is shown on the hexosaminidase HsHexB derived from the human. In a word, the berberine and the derivative of the berberine have wide application prospects in the fields of biology, chemicobiology and the like.

The invention relates the isosulfocyanate compounds (ISOTHIOCYANATES), in other word, JC-5411 compounds, which can adjust the expression of androgen acceptor gene in cell by suppressing the expression of gene transcription factor SP1, and suppress the abnormal breeding function of prostate gland cell. With the little compound, it can suppress hamster benign hypertrophy of the prostate (BPH), which is induced by suppressed male sex hormone, and the growth of prostate gland cancer cell. The invention provides the new method of effectively preventing and treating the hypertrophy of the prostate and prostate gland cancer. The compound possesses the low side effect, steady chemical biology nature, low cost of manufacture, and broad adaptability.

The invention relates to preparation and application of a near-infrared and two-photon dual-mode imaging fluorescence probe, and belongs to the technical field of chemicobiology. The near-infrared and two-photon dual-mode imaging fluorescence probe is synthesized by a nile red derivative and biotin which serve as raw materials, and the fluorescence probe is a brown product. The fluorescence probe disclosed by the invention is high in spectral quality, and respectively takes 540 nm and 590 nm as excitation wavelengths; at 660 nm, the maximum emission wavelength appears, and the fluorescence intensity is relatively high. The fluorescence probe further has a targeting effect on tumor cells, and is a targeting infrared fluorescence probe; on one hand, on the premise of not destroying a tissue sample, an in-vivo signal can be detected in real time; on the other hand, enrichment of the signal in a tumor can be enhanced, and the signal-to-noise ratio is increased. The fluorescence probe disclosed by the invention can carry out near infrared imaging and two-photon imaging; by mutual verification of the two modes, the accuracy of a result can be improved.

This invention relates to a process for the production of carboxymethyl cellulase and xylanase which comprises inoculating and growing Termitomyces clypeatus which has been deposited in the Indian Institute of Chemical Biology, Calcutta, one of the constituent laboratories of the applicant having accession no IICB-411, and at the Microbial Type Culture Collection (MTCC) at Chandigarh, India, an International Depository under the Budapest Treaty, under the accession no. MTCC 5091, in a sterilized medium containing assimilable carbon source, assimilable nitrogen source and micronutrients at a pH between 3 to 7, incubating the Termitomyces clypeatus at a temperature in the range of 25-30° C., separating the filtrate.

The invention discloses a DNA aptamer sequence of a small cell lung cancer marker gastrin releasing peptide precursor polypeptide fragment Pro-GRP31-98, and relates to the technical field of chemicobiology. The DNA aptamer sequence is obtained by screening from a single-chain DNA library through an SELEX (Systematic Evolution of Ligands by Exponential Enrichment) screening method based on an affinity magnetic bead separation method, then carrying out PCR amplification on the screened DNA aptamer library, then separating a DNA aptamer contained in the DNA aptamer library through a gene clone technology by taking escherichia coli as a recipient cell and finally sequencing various DNA aptamers through a sequencing technology. An experimental result indicates that the DNA aptamer sequence disclosed by the invention is combined with a gastrin releasing peptide precursor fragment at high affinity and high specificity.

The invention provides a method for constructing a chemical biological screening system. The method comprises the following steps: cultivating caenorhabditis elegans by using a 24-pore plate; settingdifferent experimental groups; wrapping the 24-pore plate with tin foil paper to keep away from light and prevent irradiation; putting into a shaking table and performing cultivation; after 48 or 72 hours, putting the cultivated caenorhabditis elegans into a glass slide, putting under a microscope and observing the change of the stable state of a lysosomal membrane in macrophage in the body cavityof the caenorhabditis elegans. The invention further provides a natural micromolecular compound as well as application thereof to preparation of a medicine for promoting lysosome mediated necrocytosis disease, wherein the natural micromolecular compound can promote lysosome mediated necrocytosis and is screened out according to the method for constructing the chemical biological screening system.

The invention belongs to the field of chemical biology technology, and discloses a method used for screening polypeptide in vitro. In order to break the limitation of existing polypeptide intracellular screening technology, the method comprises following steps: a random dsDNA library is constructed, and is transcribed to form mRNA; the mRNA and an oligonucleotide chain are subjected to anneal for complementation, and then in vitro expression is performed by taking the mRNA as a template, wherein the end of the oligonucleotide chain is connected with puromycin; when the expression is about to be completed, puromycin is delivered into a ribosome, a newly generated polypeptide chain is captured by puromycin and a covalent structure is formed; a random library is produced by inverse transcription, wherein in the random library, polypeptide and encoding information cDNA of the polypeptide are combined correspondingly; after screening, a primer is designed so as to subject the obtained cDNA to PCR amplification; and then a next circle of screening is performed so as to obtain the target polypeptide and encoding information of the target polypeptide after a plurality of screening cycles. The screening technologies employed in the method are in vitro, library capacity can reach 1013 to 1015, the system is stable, operation is simple, and screening efficiency is high.

The invention belongs to the field of chemicobiology, and provides a novel fluorescent dye capable of multifunctionalization, and a preparation method and application thereof. The novel fluorescent dye capable of multifunctionalization is based on a BODIPY parent, and is prepared by carrying out Knoevenagel reaction on a raw material pyrrole aldehyde for synthesizing BODIPY and propyl dicyan. The modification of the Knoevenagel reaction is utilized to achieve the goal of regulating the spectrum property of the fluorescent dye. Cl-BODIPY-2CN active chlorine can quickly perform nucleophilic substitution reaction with multiple mercapto or amino compounds, and the optical properties of the fluorescent dye obviously change in the substitution process. The Cl-BODIPY-2CN is used for detecting ONOO-. The fluorescent dye provided by the invention has the advantages of high light stability and high reaction sensitivity, and can be subjected to functionalized application in many aspects. The preparation method has the advantages of simple and accessible raw materials, low cost, higher yield and high light stability.

The present invention relates to methods for detecting sulfenylation within thiol groups in proteins, metabolites, or materials. Protein sulfenylation (Cys-SOH) describes the reversible post-translational modification of protein thiols by hydrogen peroxide, and plays a central role in oxidative signaling (see, e.g., Paulsen, C. E. & Carroll, K. S. 2013 Chemical Reviews 113, 4633-679). Growth factor stimulation activates NADPH oxidase enzymes, releasing a local burst of hydrogen peroxide, which transiently oxidizes the nuclcophilic cysteine of protein phosphatases and other proximal redox active thiols (see, e.g., Paulsen, C. E. et al., 2012 Nature Chemical Biology 8, 57-64). In addition to masking functional cysteine's, sulfenylation is also a aitical intermediate towards irreversible cysteine oxidation.

The invention belongs to the field of chemical biology, and particularly relates to a polyethyleneimine modified quantum dot particle, a preparation method thereof and an application of the polyethyleneimine modified quantum dot particle as a nano-drug carrier. The polyethyleneimine modified quantum dot nanoparticle drug carrier has a structure as shown in the following formula, in the formula, QDs refer to quantum dots CdSe or CdSe / ZnS. According to polyethyleneimine modified quantum dot nanoparticles, the particle size is small, the water solubility is, uniform dispersion is achieved, the stability is good, low cytotoxicity to various cells is achieved, so the polyethyleneimine modified quantum dot nanoparticles can be used as a good nano-drug carrier to be applied to the field of the chemical biology, and a foundation can be laid for better design of the nano-drug carrier.

The invention belongs to the field of chemical biology, and particularly relates to a nano-drug carrier of gold nanoparticles based on hyaluronic acid modification, and a preparation method and application thereof. The nano-drug carrier of the gold nanoparticles based on hyaluronic acid modification has the following general formula shown in the specification, wherein Au is the gold nanoparticlesmodified by mercaptopropionic acid. The nano-drug carrier of the gold nanoparticles based on hyaluronic acid modification in the invention has the advantages of small particle size, good water solubility, uniform dispersion, good stability and relatively low cytotoxicity to various cells at the same time, so that the nano-drug carrier can be used as a good nano-drug carrier to be applied to the field of chemical biology; and a foundation can be laid for better designing the nano-drug carrier.

The invention discloses a non-natural peptide IDH1 inhibitor synthesized based on UGI reaction as well as a preparation method and application thereof, and belongs to the technical field of chemical biology. The non-natural peptide IDH1 inhibitor synthesized based on the UGI reaction, shown in a general formula I, or pharmaceutically acceptable salt, hydrate or prodrug of the non-natural peptide IDH1 inhibitor are provided. The invention also discloses a pharmaceutical composition containing the non-natural peptide IDH1 inhibitor synthesized based on the UGI reaction. The non-natural peptide IDH1 inhibitor synthesized based on the UGI reaction can be combined with IDH1 mutant protein, inhibit the activity of IDH1 mutant enzyme and reduce the generation of 2-HG in cells, and is used for treating tumors and / or other diseases. R1, R2, R3 and R4 in the general formula I are described in the claims and the specifications.

The invention relates to a specific photochemical demethylation method for N6-methyladenine and application of N6-methyladenine. According to the method, light is used as an initiator; and under the existence of a solvent, N6-methyladenine as shown in the formula I or a derivative of N6-methyladenine reacts with a photosensitizer and an oxidant under an illumination condition, thus obtaining a demethylated product as shown in the formula II and realizing demethylation. By the use of the photochemical demethylation method for the N6-methyladenine and relevant compounds, chemical adjustment of nucleic acid under a non-enzymic condition can be adjusted. The invention provides an effective research method on nucleic acid demethylation for the field of researches on epigenetics and chemicobiology.

The invention provides a penicillamine-based polypeptide disulfide bond synthesis method. The method adopts a route 1 or 2, wherein the route 1 comprises the following steps: dissolving iodine in N,N-dimethylformamide, adding the mixture into resin A with synthesized related sequences, performing a nitrogen blowing reaction, cutting off polypeptide by using shearing liquid, and separating and purifying the mixture with high performance liquid chromatography to obtain a target product; and the route 2 comprises the following steps: cutting off the polypeptide with the synthesized related sequence from the resin A by utilizing the shearing liquid, then separating out non-oxidized and ring-closed polypeptide by using the high performance liquid chromatography, dissolving the polypeptide in a phosphate buffer solution with the pH value of 7.4-8.0, adding an oxidant into the mixture for oxidation, and carrying out separation and purification by using the high performance liquid chromatography to obtain the target product. The invention provides the novel stable disulfide bond designing and synthesizing strategy, and the disulfide bond serving as the bullet of an effective covalent reaction is applied to the field of chemical biology.