Gold nanoparticles based on hyaluronic acid modification, preparation method of gold nanoparticles, and application of gold nanoparticles as nano-drug carrier

A technology of hyaluronic acid modification and gold nanoparticles, which is applied in the field of chemical biology, can solve the problems of easy aggregation and settling together, and does not last for a long time, achieves good water solubility, improves water solubility and biological properties, and has a simple preparation method. easy effect

Inactive Publication Date: 2021-02-23
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And because the commonly synthesized gold nanoparticles can be well dispersed in aqueous ...

Method used

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  • Gold nanoparticles based on hyaluronic acid modification, preparation method of gold nanoparticles, and application of gold nanoparticles as nano-drug carrier
  • Gold nanoparticles based on hyaluronic acid modification, preparation method of gold nanoparticles, and application of gold nanoparticles as nano-drug carrier
  • Gold nanoparticles based on hyaluronic acid modification, preparation method of gold nanoparticles, and application of gold nanoparticles as nano-drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] When the ligand is 4 (PEG-HA-ADH), the synthesis steps for preparing the nano-drug carrier based on hyaluronic acid-modified gold nanoparticles are as follows:

[0037]

[0038] 1) Preparation of Compound 4: In a 250 mL round bottom flask, 1.0 g of hyaluronic acid 1 (HA, Mw=403.31, molecule weight, common commercial product) was dissolved in 30 mL of PBS (phosphate buffered saline, pH=5.0) , then add 1.742g of adipic acid dihydrazide 2 (ADH), 0.192g of 1,2-dichloroethane (EDC) and 0.119g of N-hydroxysuccinimide (NHS), nitrogen protection at room temperature Under reaction 24h. After the reaction was completed, 40 mg of polyethylene glycol 3 (PEG, Mw=2000, a common commercial product) was added to continue the reaction for 24 h under the protection of nitrogen. After the reaction, put each 6mL in a dialysis bag, take a 500mL beaker and add 500mL three-distilled water, drop concentrated hydrochloric acid into the three-distilled water to adjust the pH to 6-7, then pla...

Embodiment 2

[0046] When the ligand is 7 (PEG-HA), the synthesis steps for preparing the nano-drug carrier based on hyaluronic acid-modified gold nanoparticles are as follows:

[0047]

[0048] 1) Preparation of compound 7: In a 250 mL round bottom flask, hyaluronic acid 1 (HA, 1.0 g) was dissolved in 30 mL of PBS (pH=5.0), then PEG 3 (22 mg), EDC (7 mg) and NHS ( 10.5mg), reacted at room temperature for 24h under the protection of nitrogen. After the reaction, put each 6mL in a dialysis bag, take a 500mL beaker and add 500mL three-distilled water, drop concentrated hydrochloric acid into the three-distilled water to adjust the pH to 6-7, then place the dialysis bag (molecular weight cut-off 2000) in Put magnets in it, stir and dialyze for 48 hours with a magnet stirrer (change the dialysate every 6 hours). After dialysis, use a rotary evaporator to carry out vacuum distillation to obtain compound 7 (PEG-HA);

[0049] 2) Preparation of compound 6: refer to Example 1;

[0050] 3) Prep...

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PUM

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Abstract

The invention belongs to the field of chemical biology, and particularly relates to a nano-drug carrier of gold nanoparticles based on hyaluronic acid modification, and a preparation method and application thereof. The nano-drug carrier of the gold nanoparticles based on hyaluronic acid modification has the following general formula shown in the specification, wherein Au is the gold nanoparticlesmodified by mercaptopropionic acid. The nano-drug carrier of the gold nanoparticles based on hyaluronic acid modification in the invention has the advantages of small particle size, good water solubility, uniform dispersion, good stability and relatively low cytotoxicity to various cells at the same time, so that the nano-drug carrier can be used as a good nano-drug carrier to be applied to the field of chemical biology; and a foundation can be laid for better designing the nano-drug carrier.

Description

technical field [0001] The invention belongs to the technical field of chemical biology, and in particular relates to a hyaluronic acid-modified gold nanoparticle, a preparation method thereof, and an application as a nanomedicine carrier. Background technique [0002] Gold nanoparticles have a large surface area and a spherical shape. Nanoparticles can be used as a carrier for encapsulating many dye molecules. They have been mainly used for signal recording in fluorescence immunoassays to improve detection sensitivity. As a new type of nanomaterial, gold nanoparticles have recently turned the attention of scientists to the possibility of using gold nanoparticles for medical treatment. In view of the characteristics that the photoelectric properties of gold nanoparticles are easy to be modified, it can be used in cell detection, gene regulation drug synthesis, drug delivery, photochemical therapy, etc. Gold nanoparticles can make some special substances into cells, such as ...

Claims

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Application Information

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IPC IPC(8): A61K47/10A61K47/36A61K47/02A61K31/704A61P35/00B82Y40/00
CPCA61K47/10A61K47/36A61K47/02A61K31/704A61P35/00B82Y40/00
Inventor 赵美霞任斌甘莹李景华张亚宏蔡中超朱冰洁
Owner HENAN UNIVERSITY
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