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Process for making nano-modification minisize electrochemical biosensor for pesticide detection

A biosensor and pesticide residue detection technology, applied in the field of handheld organophosphorus pesticide detectors, can solve the problems of difficult constant measurement of potential, low detection sensitivity, small size, etc., and achieve easy on-site testing, high sensitivity, and low design cost. Effect

Inactive Publication Date: 2005-11-23
中国科学院研究生院应用化学研究所
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Problems solved by technology

[0010] In order to overcome the shortcomings of the existing two-electrode system that it is difficult to maintain a constant measurement potential, it is difficult to compare between one piece, and the error is large and the detection sensitivity is low. The present invention proposes a disposable nano-modified microelectrode for pesticide residue detection. A method for preparing a chemical biosensor. The three electrodes prepared by the method are not only small in size, easy to carry, and can provide a stable reference potential. Moreover, the nanocomposite enzyme working electrode (1), micro silver / silver chloride (Ag / AgCl) The reference electrode (2) and the counter electrode (3) are integrated to provide a simple, easy-to-use, non-destructive and easy-to-batch-produced disposable nano-modified miniature electrochemical biosensor

Method used

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  • Process for making nano-modification minisize electrochemical biosensor for pesticide detection
  • Process for making nano-modification minisize electrochemical biosensor for pesticide detection
  • Process for making nano-modification minisize electrochemical biosensor for pesticide detection

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Embodiment Construction

[0026] The printing process can be divided into several steps according to the complexity of the printed electrodes. Take the simplest three-electrode printed by us as an example. After each step of printing, it must be heated and dried in an oven for more than 24 hours before proceeding. What to do next. After all printing is completed, dry in the oven for 48 hours, and the electrodes can be used after cutting

[0027] 1. The first step is to print conductive silver paste on the substrate (6) to make the connection part (4) and wire (5) with the instrument;

[0028] 2. The second step is to print a silver-silver chloride (Ag / AgCl) layer to make a reference electrode (3).

[0029] 3. The third step is to print the conductive carbon paste to make the working electrode (1) and the counter electrode (2)

[0030] 4. The fourth step is to prepare the nanomaterial enzyme electrode (7)

[0031] 5. After the instrument is turned on and stabilized for 10 minutes, it will be tested; ...

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Abstract

The invention relates to a process for preparing allocate nanometer modification mini-electric chemical biology sensor used as pesticide residue measurement, which can provide a stable reference electric potential to measure pesticide residue in the vegetables, and an orgyanophosphorus pesticide detecting instrument for pesticide residue measurement.

Description

Technical field [0001] The invention relates to a preparation method of a disposable nano-modified micro-electrochemical biosensor for pesticide residue detection. A preparation method of an electrochemical biosensor and a handheld organophosphorus pesticide detector for pesticide residue detection. Background technique [0002] Organophosphorus and carbamate pesticides are currently the most used pesticides in my country, and most of them are used in limited quantities on vegetable and fruit crops. Some highly toxic pesticides are prohibited from being used on vegetables and fruits. But no matter how strict the registration system and management regulations are, there are always people who violate the regulations and abuse pesticides. Since such pesticides are likely to remain in large quantities on the surface of fruits and vegetables, they can cause nerve conduction blockage, nerve paralysis and death in humans. Therefore, it is of great significance to quickly measure...

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Application Information

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IPC IPC(8): G01N27/403
Inventor 张君黄鸿年袁倬斌
Owner 中国科学院研究生院应用化学研究所
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