33 results about "Hexosaminidase" patented technology

The invention discloses a development culture medium for separating and identifying pathogens in a urogenital tract, which at least comprises three development substrates, namely a hexosaminidase substrate, a beta-D-galactosidase substrate, a beta-D-glucuroide substrate. The development culture medium added with the three development substrates is prepared into a microbial culture medium agar plate, a sample or a bacterial colony subjected to separate culture is inoculated into the development plate and is incubated, and a result can be directly observed. In the development culture medium, various pathogens including bacteria and fungi in a genital tract can be simultaneously cultured and identified on the same development plate, namely bacterial pathogens such as Escherichia coli, Klebsiella pneumonia, enterococcus, pseudomonas aeruginosa, proteus mirabilis, staphylococcus aureus and the like and fungal pathogens such as candida albicans, candida tropicalis and the like can be simultaneously identified, and judgment can be performed through visual inspection; and the development culture medium is quick and convenient to use, and easy to operate.

Disclosed are compositions and methods related to delivering a nucleic acid to the central nervous system.

The invention discloses application of berberine and a derivative of the berberine as a hexosaminidase inhibitor. The general structure formula of the hexosaminidase inhibitor is shown as I or II; in all screened 35 compounds, the berberine and the derivative of the berberine show certain inhibition activity on hexosaminidase OfHex1; particularly, the suppression rate of Sysu-00021 and Sysu-00679 on the OfHex1 enzyme is respectively 76 percent and 79 percent, and the inhibition constant Ki is respectively 2mu M and 1.8mu M. Compound applicable object determination shows that under the final concentration of 10mu M, the compound shows higher inhibition activity on the hexosaminidase OfHex1; no inhibition activity is shown on the hexosaminidase HsHexB derived from the human. In a word, the berberine and the derivative of the berberine have wide application prospects in the fields of biology, chemicobiology and the like.

A method, and associated kit, for assaying the activity of lysosomal enzymes present in dried bodily fluids and cell tissue samples, such as α-L-iduronidase, β-D-galactosidase, β-D-glucosidase, chitotriosidase, total α-D-galactosidase and α-D-galactosidase A, hexosaminidase A and B, α-D-mannosidase, β-D-mannosidase, α-L-fucosidase, N-acetyl-α-galactosaminidase, arylsulfatases, sphingomyelinase, β-galactocerebrosidase, iduronate-2-sulfatase and β-D-glucuronidase. The method includes: (a) combining with a dried bodily fluid or cell tissue sample containing at least one type of lysosomal enzyme: (1) an eluent, (2) an incubation buffer and (3) a substrate or substrates capable of reacting with the assayed lysosomal enzymes and producing their corresponding enzyme product or products, (b) allowing the dried bodily fluid or cell tissue sample to react with the eluent, incubation buffer and substrate or substrates for an adequate time and temperature, and (c) applying measuring means to the enzyme product to determine the activities of the lysosomal enzymes present.

The present invention discloses two hexosaminidase gene sequences derived from strawberries (Fragaria x ananassa qing xia) and a preparation method and an application thereof, and a technical method using genetic engineering. The hexosaminidase gene is cloned into a pichia pastoris expression vector to obtain a pichia pastoris recombinant strain heterologously expressing hexosaminidase, abd the hexosaminidase prepared by the heterologous expression of the strain can uses p-nitrophenyl-N-acetyl-beta-D-amino glucosamine or p-nitrophenyl-N-acetyl-beta-D-aminogalactosamine as a substrate for reaction. The present invention also provides the application of the hexosaminidase in controlling postharvest fruit softening and preservative activity screening, and belongs to the field of fruit preservation.

The present invention provides glucoimidazole derivatives and methods of making them. The compounds can be used to inhibit the activity of O-GlcNAcase enzymes, including both bacterial OGA (bOGA) and human OGA (hOGA) and can be selective, showing low inhibition of hexosaminidases. The compounds can be used to study the role of the O-GlcNAcase modification in human or animal cells. Furthermore the compounds can have therapeutic uses in the treatment of diseases mediated by the activity of O-GlcNAcase enzymes including type II diabetes, Alzheimers Disease, and cancer.

The composition according to the present invention, which comprises Lactococcus chungangensis as an active ingredient, has excellent effects of preventing or treating inflammatory diseases, inhibiting the secretion of nitric oxide and prostaglandin E2, which are major inflammatory factors, and inhibiting the secretion of β-hexosaminidase and histamine, which are major factors related to allergies, and also significantly suppressing the production of skin disease-related cytokines and chemokines. Such effects are at the same level as those of conventional skin disease therapeutic agents (tacrolimus), and thus the composition can be used as a preventive or therapeutic agent against inflammatory diseases. In addition, the composition according to the present invention exhibits an antibacterial activity against Staphylococcus aureus, which is a microorganism inducing a secondary infection of atopic dermatitis and the like, and thus can be used in preventing or treating a bacterial infection.

The invention relates to candida chromogenic medium, a detection kit and a detection method, belonging to the technical field of microbial diagnosis, in particular to the cultivation and identification of yeast in medical microbiology specimens, drugs and medical equipment sanitary inspection samples, public health surveillance samples and food (including cosmetics) sanitary inspection samples. The chromogenic medium of the invention is composed of basic medium, mixed chromogenic substrate and bacteriostat, wherein the mixed chromogenic substrate consists of aminocaproic glucosidase and alkaline phosphatase substrate, and candida specific enzyme is added into the mixed chromogenic substrate. The detection kit of the invention consists of the chromogenic medium, identification paper A containing enzyme substrate 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-aminogalactose and identification paper B containing enzyme substrate 5-bromo-4-chloro-3-indolyl-beta-D- glycopyranoside. In the invention, the candida chromogenic medium and the detection kit have the advantages of low cost and simple configuration, and the method can be applied to the separation and identification of candida rapidly, simply and accurately.

The invention discloses a vaginal secretion dry chemical analysis liquid quality control material and a preparation method thereof. A corresponding buffer system is prepared first, and then, with the buffer system as a solvent, a plurality of raw materials of a negative quality control material and a positive quality control material are added and dissolved; when different components are added, the next component can be added after the previous component is completely dissolved; in order to ensure the enzyme activity, a liquid negative quality control material and a liquid positive quality control material are prepared in an environment of 4-15 DEG C, and the effect is better; after the preparation is completed, subpackaging and storage are carried out within 24 hours, and quality control can be carried out on 10 items such as coagulase, hydrogen peroxide, lactic acid, oxidase, glucuronidase, N-acetyl hexosaminidase, sialidase, leukocyte esterase, proline aminopeptidase and pH value at the same time, so that the stability is good, the period is long, the use is convenient and fast, complex step of redissolving before the freeze-dried product is used is omitted, and errors caused by redissolving are reduced; and the liquid quality control material has a good practical application value.

The composition according to the present invention, which comprises Lactococcus chungangensis as an active ingredient, has excellent effects of preventing or treating inflammatory diseases, inhibiting the secretion of nitric oxide and prostaglandin E2, which are major inflammatory factors, and inhibiting the secretion of β-hexosaminidase and histamine, which are major factors related to allergies, and also significantly suppressing the production of skin disease-related cytokines and chemokines. Such effects are at the same level as those of conventional skin disease therapeutic agents (tacrolimus), and thus the composition can be used as a preventive or therapeutic agent against inflammatory diseases. In addition, the composition according to the present invention exhibits an antibacterial activity against Staphylococcus aureus, which is a microorganism inducing a secondary infection of atopic dermatitis and the like, and thus can be used in preventing or treating a bacterial infection.

The invention discloses application of a compound for efficient inhibition of chitinase OfChi-h and hexosaminidase OfHex1 activity, and relates to application of a compound phlegmacin B1 and its derivatives in inhibiting chitinase OfChi-h and hexosaminidase OfHex1 activity. The used final concentration is not less than 13ppm, under the concentration, the measured inhibition rates are 90.4% and 71.4% respectively, and inhibition constants Ki are 5.5 microM and 26 microM respectively. The invention proves that the compound phlegmacin B1 has good application prospect in the prevention and control of agricultural pests, especially in prevention and control of lepidoptera insects.

Certain attenuated mutants of Brucella, especially B. melitensis, B. abortus, B. suis and B. ovis, when administered to a human or animal trigger a protective immune response such that subsequent challenge with virulent Brucella of the same species does not result in disease or results in much less severe symptoms. Functional inactivation of galE, a virB gene or the operon (ORFs 1087-1090) comprising the gene encoding β-hexosaminidase (BMEI1087) and a lytic murein transglycosylase gene (BMEI1088). A specific example of the attenuated galE mutant which produces a protective immune response is B. melitensis GR024. The specific example of an inactivated ORF1087-1090 operon is B. melitensis GR026; it has an insertion mutation in the promoter region upstream of ORF 1090. Vaccination with live cells of either or both of these mutants results in a T cell response which protects the human or animal against challenge with virulent B. melitensis. Similar strategies for protective immunity using live attenuated mutants are useful for B. abortus, B. suis and B. ovis as well.

The invention provides a quinazolinone-containing diaryl urea compound and a preparation method and application thereof. The quinazolinone-containing diaryl urea compound can obviously inhibit LAD2 cells from releasing beta-hexosaminidase, shows obvious dosage association, can be used for preparing an antiallergic medicament, and particularly can be used for preparing a medicament for antagonizingLAD2 cells from releasing beta-hexosaminidase so as to reduce pain and a load of a patient. The preparation method provided by the invention is easy in obtaining of raw material sources, mild in reaction condition, simple in operation in the reaction process and cheap in used reagent.

Aspects of the disclosure relate to bicistronic AAV nucleic acid constructs comprising a transgene encoding hexosaminidase A ( HEXA ) and hexosaminidase (HEXB) proteins. In some embodiments, the disclosure provides methods for treating or preventing lysosomal storage disorders, such as Tay-Sachs disease and Sandhoff disease, using bicistronic nucleic acid constructs described by the disclosure.

The invention discloses two hexosaminidase gene sequences derived from strawberry (Fragaria×ananassa qing xiang) and its preparation method and application, that is, using the technical method of genetic engineering to clone the hexosaminidase gene into Pichia On the yeast expression vector, a Pichia recombinant strain capable of heterologously expressing the enzyme is obtained, and the hexosaminidase prepared by heterologously expressing the strain can be p-nitrophenyl-N-acetyl-β-D-amino Glucosamine or P‑nitrophenyl‑N‑acetyl‑β‑D‑galactosamine as substrates for the reaction. The invention also provides the application of the hexosaminidase in controlling post-harvest fruit softening and preservative activity screening, which belongs to the field of fruit preservation.